CN110946071A - Rice breeding method and breeding germination equipment - Google Patents

Rice breeding method and breeding germination equipment Download PDF

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Publication number
CN110946071A
CN110946071A CN201911285527.2A CN201911285527A CN110946071A CN 110946071 A CN110946071 A CN 110946071A CN 201911285527 A CN201911285527 A CN 201911285527A CN 110946071 A CN110946071 A CN 110946071A
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China
Prior art keywords
rice
box
germination
breeding
generation
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CN201911285527.2A
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Chinese (zh)
Inventor
阳标仁
王子平
黄志才
刘欢欢
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HUNAN RICE RESEARCH INSTITUTE
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HUNAN RICE RESEARCH INSTITUTE
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Priority to CN201911285527.2A priority Critical patent/CN110946071A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/02Germinating apparatus; Determining germination capacity of seeds or the like
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physiology (AREA)
  • Soil Sciences (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention belongs to the technical field of rice breeding, and particularly relates to a rice breeding method and breeding germination equipment. The top end of the germination box is provided with a sealing top cover, and the lower end of the sealing top cover is fixed with a light supplementing and heat dissipating lamp; the left side wall and the right side wall of the germination box are provided with air-permeable grids; the lower end of the inner side of the cultivation germination box is provided with a convex edge rail, the convex edge rail is movably connected with a cultivation box through the matching of a clamping groove, and the right end of the cultivation box is provided with a rubber sealing end cover; the rubber sealing end cover is in sealing connection with the side wall of the germination box, and a circulating hole groove is formed in the outer side of the rubber sealing end cover. The method carries out selfing after repeated hybridization of a high-quality variety after hybridization and a characteristic male parent, and transfers the characteristics of the parent to a new variety of rice, so that the new variety of rice can be more suitable for growth in a complex environment; in addition, the survival rate of hybridization and selfing is improved by matching with special germination equipment.

Description

Rice breeding method and breeding germination equipment
Technical Field
The invention belongs to the technical field of rice breeding, and particularly relates to a rice breeding method and breeding germination equipment.
Background
The grain of China mainly comprises rice and wheat, wherein the rice occupies a large part, and the research on the rice technology needs to be strengthened when the rice yield is improved. The rice technology in China is obviously improved in recent years, but some problems which need to be further solved still exist, and the problems seriously influence the development of the rice technology in China.
The conditions of long seed dormancy period, low germination rate, irregular germination, low germination quality and the like of rice seeds caused by external environment or self growth factors seriously influence the growth of later-stage crops, and lead to a series of problems of long production period, poor product quality and the like. Because the seeds carry bacteria and plant pathogenic bacteria exist commonly in soil, diseases such as bakanae disease and damping off occur in the rice seedling raising process, so that the diseases occur commonly in the seedling stage. In northern areas of China, particularly in cold areas, rice seeds are sensitive to temperature and have long germination time, so that the situation that the growth vigor of the rice seedlings is inconsistent often occurs. The change of weather conditions such as late spring and cold also can cause adverse effect on the establishment of the seedlings.
Therefore, how to increase the emergence rate of rice seeds and promote the growth of rice becomes a technical problem to be solved urgently in the field.
Disclosure of Invention
Aiming at solving the problems of the defects and the shortcomings of the prior art; the invention aims to provide a rice breeding method and breeding and sprouting equipment which are simple in structure, reasonable in design and convenient to use; the method carries out selfing after repeated hybridization of a high-quality variety after hybridization and a characteristic male parent, and transfers the characteristics of the parent to a new variety of rice, so that the new variety of rice can be more suitable for growth in a complex environment; in addition, the survival rate of hybridization and selfing is improved by matching with special germination equipment.
In order to achieve the purpose, the invention adopts the technical scheme that: the cultivation method comprises the following steps:
s1: selecting a characteristic rice variety as a male parent A, and selecting a restorer line rice variety as a female parent B; and breeding excellent single plant in the segregation population;
s2: to two selected single rice plantsCarrying out breeding, hybridization and matching to obtain first-generation hybrid F1 generation seed A1B1F1, screening the hybrid seeds of the F1 generation, cultivating and germinating in a cultivating and germinating box, selecting 500-1000 plants for planting and selfing to form the selfing F2 generation A1B1F2 and selfing F3 generation A1B1F3;
S3: selecting the three generations of inbred F3A in S21B1F3, the plants with excellent characteristics are hybridized with the male parent A to form two F4 generations A2B2F4, selfing the F4 generation for two generations to obtain a selfed F6 generation A2B2F6;
S4: selecting inbred F6 generation A in S32B2F6 is only required to be a plant seed with excellent characteristics.
Preferably, the crosses and selfings of S2 and S3 are both bred in relatively independent environments.
The rice breeding and sprouting equipment comprises a sprouting box, a sealing top cover, a light supplementing and heat dissipating lamp, a ventilating grille, a convex edge rail, a clamping groove, a sprouting box and a rubber sealing end cover; the top end of the germination box is provided with a sealing top cover, and the lower end of the sealing top cover is fixed with a light supplementing and heat dissipating lamp; the left side wall and the right side wall of the germination box are provided with air-permeable grids; the lower end of the inner side of the cultivation germination box is provided with a convex edge rail, the convex edge rail is movably connected with a cultivation box through the matching of a clamping groove, and the right end of the cultivation box is provided with a rubber sealing end cover; the rubber sealing end cover is in sealing connection with the side wall of the germination box, and a circulating hole groove is formed in the outer side of the rubber sealing end cover.
Preferably, the inside of the ventilating grille is provided with a rotary fan, and one side of the ventilating grille at two sides of the cultivating and germinating box is provided with air inlet and the other side of the ventilating grille is provided with air exhaust, so that air circulation is realized.
Preferably, the inside of the incubation box is filled with a seed soaking gel.
Preferably, the seed soaking gel consists of a gel carrier, 25% of prochloraz, a plant growth regulator, an antifoaming agent and a proper amount of water.
Preferably, the surface of the seed soaking gel is grooved by a cutter, and rice seeds are inlaid in the grooves.
Preferably, the inner aqueous solution of the said cultivating box is kept flush with the upper surface of the seed soaking gel, and it is circulated with running water through the circulation hole groove.
After adopting the structure, the invention has the beneficial effects that: the method carries out selfing after repeated hybridization of a high-quality variety after hybridization and a characteristic male parent, and transfers the characteristics of the parent to a new variety of rice, so that the new variety of rice can be more suitable for growth in a complex environment; in addition, the survival rate of hybridization and selfing is improved by matching with special germination equipment.
Drawings
To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the present invention is described in detail by the following specific embodiments and the accompanying drawings.
FIG. 1 is a schematic view of the structure of the germination box of the present invention;
FIG. 2 is a schematic view of the surface cutting grooves of the seed soaking gel of the present invention.
Description of reference numerals: cultivate case 1 that sprouts, sealing top cap 2, light filling heat dissipation lamp 3, ventilative grid 4, bead track 5, draw-in groove 6, cultivate box 7, rubber seal end cover 8, soak kind of gel 9, circulation hole groove 10.
Detailed Description
In order that the objects, aspects and advantages of the invention will become more apparent, the invention will be described by way of example only, and in connection with the accompanying drawings. It is to be understood that such description is merely illustrative and not intended to limit the scope of the present invention. Moreover, in the following description, descriptions of well-known structures and techniques are omitted so as to not unnecessarily obscure the concepts of the present invention.
It should be noted that, in order to avoid obscuring the present invention with unnecessary details, only the structures and/or processing steps closely related to the scheme according to the present invention are shown in the drawings, and other details not so relevant to the present invention are omitted.
The specific implementation mode adopts the following technical scheme:
the first embodiment is as follows:
the cultivation method comprises the following steps:
s1: selecting a characteristic rice variety as a male parent A, and selecting a restorer line rice variety as a female parent B; and breeding excellent single plant in the segregation population;
s2: breeding, hybridizing and matching two selected single rice plants to obtain first-generation hybrid F1-generation seeds A1B1F1, screening the hybrid seeds of the F1 generation, cultivating and germinating in a cultivating and germinating box, selecting 500-1000 plants for planting and selfing to form the selfing F2 generation A1B1F2 and selfing F3 generation A1B1F3;
S3: selecting the three generations of inbred F3A in S21B1F3, the plants with excellent characteristics are hybridized with the male parent A to form two F4 generations A2B2F4, selfing the F4 generation for two generations to obtain a selfed F6 generation A2B2F6;
S4: selecting inbred F6 generation A in S32B2F6 is only required to be a plant seed with excellent characteristics.
Preferably, the crosses and selfings of S2 and S3 are both bred in relatively independent environments.
Referring to fig. 1 and 2, the rice breeding and sprouting equipment comprises a sprouting box 1, a sealing top cover 2, a light supplementing and heat dissipating lamp 3, a ventilating grille 4, a rib rail 5, a clamping groove 6, a sprouting box 7 and a rubber sealing end cover 8; the top end of the germination box 1 is provided with a sealing top cover 2, and the lower end of the sealing top cover 2 is fixed with a light supplementing and heat dissipating lamp 3; the left side wall and the right side wall of the germination box 1 are provided with air-permeable grids 4; the lower end of the inner side of the cultivating and germinating box 1 is provided with a convex edge rail 5, the convex edge rail 5 is movably connected with a cultivating box 7 through the matching of a clamping groove 6, and the right end of the cultivating box 7 is provided with a rubber sealing end cover 8; the rubber sealing end cover 8 is in sealing connection with the side wall of the germination box 1, and a circulating hole groove 10 is arranged on the outer side of the rubber sealing end cover 8.
Wherein, a rotary fan is arranged in the ventilating grille 4, and one side of the ventilating grille 4 at two sides of the cultivating and germinating box 1 is provided with air inlet and the other side is provided with air outlet, so that air circulation is realized; the inside of the cultivating box 7 is filled with seed soaking gel 9; the seed soaking gel 9 consists of a gel carrier, 25 percent of prochloraz, a plant growth regulator, a defoaming agent and a proper amount of water; the surface of the seed soaking gel 9 is grooved by a cutter, and rice seeds are inlaid in the grooves; the inner aqueous solution of the said cultivating box 7 is kept flush with the upper surface of the seed soaking gel 9, which is circulated with running water through the circulation hole groove 10.
In addition, the light supplementing and heat dissipating lamp 3 is a low temperature heating lamp tube, the highest heating temperature is 30 ℃, and a distance of 80-100cm is kept between the light supplementing and heat dissipating lamp 3 and the cultivation box 7.
After adopting the structure, the invention has the beneficial effects that: the method carries out selfing after repeated hybridization of a high-quality variety after hybridization and a characteristic male parent, and transfers the characteristics of the parent to a new variety of rice, so that the new variety of rice can be more suitable for growth in a complex environment; in addition, the survival rate of hybridization and selfing is improved by matching with special germination equipment.
The second embodiment is as follows:
the cultivation method comprises the following steps:
s1: selecting a characteristic rice variety as a male parent A, and selecting a restorer line rice variety as a female parent B; and breeding excellent single plant in the segregation population;
s2: breeding, hybridizing and matching two selected single rice plants to obtain first-generation hybrid F1-generation seeds A1B1F1, screening the hybrid seeds of the F1 generation, cultivating and germinating in a cultivating and germinating box, selecting 500-1000 plants for planting and selfing to form the selfing F2 generation A1B1F2 and selfing F3 generation A1B1F3;
S3: selecting the three generations of inbred F3A in S21B1F3 plant with excellent characteristics and male parent A and F1 generation hybrid seed A1B1The mixed breeding of the three F1 generations to form a new F41 generation A2B2F41, selfing the F41 generation for two generations to obtain a selfed F61 generation A2B2F61;
S4: selecting inbred F61 generation A in S32B2Plant with excellent F61 characteristicAnd (4) seed treatment.
The third concrete implementation mode:
the difference of the present embodiment lies in; the seed soaking gel 9 in the rice breeding germination equipment can be replaced by a cotton bed and a seed soaking hydrosolvent, so that the seed soaking solution can be circularly purified conveniently, and the cotton bed and the seeds are laid layer by layer, so that the large-batch germination requirements can be met.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims (8)

1. A rice breeding method is characterized in that: the cultivation method comprises the following steps:
s1: selecting a characteristic rice variety as a male parent A, and selecting a restorer line rice variety as a female parent B; breeding excellent single plants in the segregating population for breeding and seed selection;
s2: breeding, hybridizing and matching two selected single rice plants to obtain first-generation hybrid F1-generation seeds A1B1F1, screening the hybrid seeds of the F1 generation, cultivating and germinating in a cultivating and germinating box, selecting 500-1000 plants for planting and selfing to form the selfing F2 generation A1B1F2 and selfing F3 generation A1B1F3;
S3: selectingInbred three generations of F3 generation A in S21B1F3, the plants with excellent characteristics are hybridized with the male parent A to form two F4 generations A2B2F4, selfing the F4 generation for two generations to obtain a selfed F6 generation A2B2F6;
S4: selecting inbred F6 generation A in S32B2F6 is only required to be a plant seed with excellent characteristics.
2. A method of breeding rice as claimed in claim 1, wherein: the hybridization and selfing of the S2 and S3 are carried out in relatively independent environments.
3. A rice breeding equipment that sprouts which characterized in that: the cultivation box comprises a cultivation germination box (1), a sealing top cover (2), a light supplementing and heat dissipating lamp (3), a ventilating grille (4), a rib rail (5), a clamping groove (6), a cultivation box (7) and a rubber sealing end cover (8); a sealing top cover (2) is arranged at the top end of the germination box (1), and a light supplementing and heat dissipating lamp (3) is fixed at the lower end of the sealing top cover (2); the left side wall and the right side wall of the germination box (1) are provided with ventilating grilles (4); the lower end of the inner side of the cultivation germination box (1) is provided with a convex edge rail (5), the convex edge rail (5) is movably connected with a cultivation box (7) through the matching of a clamping groove (6), and the right end of the cultivation box (7) is provided with a rubber sealing end cover (8); the rubber sealing end cover (8) is in sealing connection with the side wall of the germination box (1), and a circulating hole groove (10) is formed in the outer side of the rubber sealing end cover (8).
4. The rice breeding germination apparatus of claim 3, wherein: the inside rotary fan that is provided with of ventilative grid (4), and cultivate ventilative grid (4) one side of sprouting box (1) both sides and admit air the opposite side and exhaust, realize air cycle.
5. The rice breeding germination apparatus of claim 3, wherein: the interior of the culture box (7) is filled with seed soaking gel (9).
6. A rice breeding germination apparatus as claimed in claim 5, wherein: the seed soaking gel (9) consists of a gel carrier, 25% of prochloraz, a plant growth regulator, a defoaming agent and a proper amount of water.
7. A rice breeding germination apparatus as claimed in claim 5, wherein: the surface of the seed soaking gel (9) is grooved through a cutter, and rice seeds are inlaid in the grooves.
8. The rice breeding germination apparatus of claim 3, wherein: the inner water solution of the culture box (7) is kept flush with the upper surface of the seed soaking gel (9), and the inner water solution is circulated by the circulating hole groove (10).
CN201911285527.2A 2019-12-13 2019-12-13 Rice breeding method and breeding germination equipment Pending CN110946071A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112616652A (en) * 2020-12-23 2021-04-09 广西壮族自治区农业科学院 Method for quickly creating rice breeding material and breeding equipment

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6240674B1 (en) * 1996-03-01 2001-06-05 Honda Giken Kogyo Kabushiki Kaisha Seedling raising sheet, method of manufacturing same, seedling raising method, culturing method, and transplanter
CN102948361A (en) * 2011-08-24 2013-03-06 湖南杂交水稻研究中心 Hybrid rice breeding method suitable for mechanical harvesting of seeds
JP2013070693A (en) * 2011-09-26 2013-04-22 Masayoshi Tsumura Sprout cultivator
CN204499019U (en) * 2015-03-18 2015-07-29 刘晓冰 Agricultural seed breeding arrangement
CN206564795U (en) * 2017-03-13 2017-10-20 连云港市农业科学院 A kind of rice seed germination machine
CN108966746A (en) * 2018-08-03 2018-12-11 马率峰 A kind of Biology Breeding seed cultivation device
CN209299838U (en) * 2018-12-27 2019-08-27 乐山师范学院 A kind of rice seed germination device for raising seedlings

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6240674B1 (en) * 1996-03-01 2001-06-05 Honda Giken Kogyo Kabushiki Kaisha Seedling raising sheet, method of manufacturing same, seedling raising method, culturing method, and transplanter
CN102948361A (en) * 2011-08-24 2013-03-06 湖南杂交水稻研究中心 Hybrid rice breeding method suitable for mechanical harvesting of seeds
JP2013070693A (en) * 2011-09-26 2013-04-22 Masayoshi Tsumura Sprout cultivator
CN204499019U (en) * 2015-03-18 2015-07-29 刘晓冰 Agricultural seed breeding arrangement
CN206564795U (en) * 2017-03-13 2017-10-20 连云港市农业科学院 A kind of rice seed germination machine
CN108966746A (en) * 2018-08-03 2018-12-11 马率峰 A kind of Biology Breeding seed cultivation device
CN209299838U (en) * 2018-12-27 2019-08-27 乐山师范学院 A kind of rice seed germination device for raising seedlings

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112616652A (en) * 2020-12-23 2021-04-09 广西壮族自治区农业科学院 Method for quickly creating rice breeding material and breeding equipment

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Application publication date: 20200403