CN110938672A - Method for testing microbial drug sensitivity test by using drug sensitivity indicator - Google Patents
Method for testing microbial drug sensitivity test by using drug sensitivity indicator Download PDFInfo
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2304/00—Chemical means of detecting microorganisms
- C12Q2304/20—Redox indicators
- C12Q2304/22—Resazurin; Resorufin
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Abstract
The invention relates to a method for testing microbial drug sensitivity by using a drug sensitivity indicator, which takes resazurin or soluble resazurin salts thereof as the drug sensitivity indicator and indicates the result of the microbial drug sensitivity test by color and/or fluorescent signals. After the drug sensitivity indicator is combined with a drug sensitivity test, the sensitivity of the drug sensitivity test can be increased, the detection time of the drug sensitivity test is shortened, and the drug sensitivity indicator has important clinical guidance significance and great application value.
Description
Technical Field
The invention relates to a microbial drug sensitivity test, in particular to a test method for performing the microbial drug sensitivity test by using a drug sensitivity indicator.
Background
In recent years, because a large number of antibacterial drugs are widely used clinically, the drug resistance of bacteria is more and more common, some bacteria can simultaneously resist 2-7 drugs, the wide spread of drug-resistant strains brings great difficulty to clinical treatment, and in addition, clinical diagnosis and treatment means are continuously updated, so that diagnosis and treatment effects are improved, but more cross-infection opportunities are brought, and the immune state of an organism tends to be low. Of particular interest is the large shift in infection types, yet changes, which places greater demands on the clinical detection of microbial drug sensitivity.
At present, the domestic methods for detecting the drug sensitivity of microorganisms mainly comprise a paper diffusion method and a dilution method. The paper diffusion method is to stick the filter paper containing quantitative antibacterial drugs on the surface of the agar inoculated with the test bacteria, and judge the MIC value of the drugs to the microorganisms according to the growth condition of the bacteria, and the method has the defects of one-time detection of few drugs and incapability of quantifying the MIC value in clinical tests. The dilution method is to dilute the concentration of the antibacterial drug by multiple ratios, wherein the lowest drug concentration capable of inhibiting the visible growth of the flesh and eyes of the bacteria to be detected is the Minimum Inhibitory Concentration (MIC). The dilution method is usually carried out through a microbial drug sensitive plate, but the products in China have certain defects: (1) the culture time of the drug sensitivity test is longer; (2) the growth condition of the microorganism is judged according to the turbidity of the drug sensitivity test result, and the judgment index is not obvious.
The drug sensitive test indicator is a substance which can be added into a drug sensitive test reaction to increase the sensitivity of the drug sensitive test and the convenience of interpretation, does not influence the growth of microorganisms and the drug effect of antibacterial drugs, and can convert turbidity signals generated by the growth of the microorganisms into more easily identified color signals and fluorescence signals, thereby increasing the sensitivity of the drug sensitive test and enabling the signal identification of a drug sensitive reading instrument to be easier.
Disclosure of Invention
The invention aims to solve the problems and provide a method for testing microbial drug sensitivity tests by using a drug sensitivity indicator, which improves the interpretation sensitivity of the drug sensitivity tests and increases the specificity of the interpretation results and can meet the detection requirements of various types of microbial drug sensitivity tests.
The purpose of the invention is realized by the following technical scheme:
a method for detecting drug sensitivity of microorganism by using drug sensitivity indicator comprises using resazurin or its soluble salt as drug sensitivity indicator, and indicating drug sensitivity result of microorganism by color and/or fluorescence signal.
Further, the result of indicating drug sensitivity by color is specifically that if the color of the indicator is red, pink or purple, the indicator represents the growth of microorganisms in the drug sensitivity test, and the antibacterial drug does not work;
if the indicator is blue, bluish purple or purple, it means that the microorganism does not grow in the drug sensitive test, and the antibacterial drug acts.
Further, the indication of the drug sensitivity result through the fluorescence signal is specifically that the indicator is irradiated by excitation light, if a strong fluorescence signal appears, the indicator represents that the microorganism grows in the drug sensitivity test, the antibacterial drug does not act, and if no strong fluorescence signal appears, the indicator represents that the microorganism does not grow in the drug sensitivity test, and the antibacterial drug acts.
Furthermore, the excitation light is the excitation light with the wavelength of 500-540 nm, and the fluorescence signal with the wavelength of 570-620 nm is collected.
Furthermore, the resazurin or the soluble resazurin salt thereof can be coated into a drug sensitive reagent in advance to form one of the components of the drug sensitive reagent, and can also be added in the drug sensitive test process as an independent reagent.
Furthermore, one specific method for coating the resazurin or the soluble resazurin salt thereof into the drug-sensitive reagent in advance to become the component of the drug-sensitive reagent is to directly add the resazurin or the soluble resazurin salt into the drug-sensitive reagent.
Furthermore, the specific method of the resazurin or the soluble resazurin salts thereof as the independent reagent is as follows,
when a drug susceptibility test is carried out, resazurin or soluble resazurin salts are prepared into a solution and added into a drug susceptibility inoculation culture solution;
or in the drug susceptibility test culture stage, preparing the resazurin or soluble resazurin salts into a solution, and adding the solution into a drug susceptibility test reaction hole;
or preparing solution of resazurin or soluble resazurin salt 15 min-2 h before the result of the drug sensitivity test is judged, and adding the solution into the reaction hole of the drug sensitivity test.
Further, the resazurin or the soluble resazurin salt is added in an amount to ensure that the concentration of the resazurin or the soluble resazurin salt is 5 to 100 mu g/mL in a drug sensitivity test.
Further, the microorganism includes, but is not limited to, bacteria and/or fungi.
The invention uses resazurin or its soluble resazurin salt as the indicator of drug sensitive test, which does not affect the growth of microbe and the drug effect of antibacterial drug, and can convert the turbidity signal of microbe growth into more recognizable color signal and fluorescence signal, thus increasing the sensitivity of drug sensitive test and making the signal recognition of drug sensitive reading instrument easier.
After the drug sensitivity indicator is combined with a drug sensitivity test, the sensitivity and the detection time of the drug sensitivity test can be increased, so that the clinical guidance significance is important, and the application value is greater.
The inventor improves the turbidity signal of the traditional turbidimetry, so that the turbidity signal is converted into a color source signal or a fluorescence signal, the detection of an automatic instrument is more convenient, and the detection sensitivity of the instrument is increased.
Compared with a trace broth dilution method provided by CLSI M100, the consistency of the results reaches 95 percent, and the international standard is reached.
Compared with the prior art, the method synchronously judges the survival condition of bacteria by utilizing the color/fluorescence signals, and when a drug sensitivity test is carried out, because the concentration of the antibacterial drug is subjected to gradient dilution, the concentration of partial antibacterial drug is not enough to completely kill microorganisms, so that the indicator presents intermediate purple.
Detailed Description
The antibiotics and reagents used in the examples are commercially available.
The antibiotic information used is as follows: all antibiotic specifications were 100 mg/count.
All antibiotics were purchased from the chinese institute for drug and biological products. The sodium salt of Resazurin was obtained from SIGMA. Enterobacteriaceae drug sensitive plate (ME2) was purchased from Shanghai Bai Biotech Co., Ltd, and the drug sensitive inoculation culture solution was purchased from Shanghai Bai Biotech Co., Ltd.
Example 1
The drug sensitive indicator is matched with a drug sensitive strip for use:
1) 5.0mg of the sodium celastrium salt is weighed, purified water is added to prepare a solution of 5mg/ml, and the solution is filtered by a sterile filter membrane of 0.22 mu m for later use.
2) 72 mu L of prepared 5mg/ml solution of celastrus angulatus sodium salt is added into the drug sensitive inoculation culture solution.
3) The test was carried out using the enterobacteriaceae drug sensitive plate (ME2) according to the instruction of the enterobacteriaceae drug sensitive plate (ME2) and using the drug sensitive inoculation culture solution containing the solution of Resazurin sodium salt added in step 2) instead of the original drug sensitive inoculation culture solution.
4) After 20 hours of incubation, the enterobacteriaceae drug-sensitive plate was removed for use as follows: 1) and (4) reading the result by visual inspection, wherein if the color of the indicator is red, pink or purple, the indicator represents the growth of microorganisms in the drug sensitivity test, and the antibacterial drug does not work. If the indicator is blue, bluish purple or purple, it means that the microorganism does not grow in the drug sensitive test, and the antibacterial drug acts. 2) And (3) judging the result of the fluorescence signal: exciting with 530nm exciting light, collecting 590nm fluorescence signal, comparing the collected signal with the negative control hole and the positive signal hole signal, if the test hole signal value is larger than (negative hole signal + positive hole signal)/2, the test hole is judged to be positive, otherwise, the test hole is judged to be negative.
Example 2
The drug sensitive indicator is matched with a drug sensitive strip for use:
1) 5.0mg of the sodium celastrium salt is weighed and added with purified water to prepare 0.5mg/ml solution which is filtered by a sterile filter membrane with the diameter of 0.22 mu m for standby.
2) The enterobacteriaceae drug-sensitive plate (ME2) was used according to the instructions for the use of the enterobacteriaceae drug-sensitive plate (ME 2).
3) After 20 hours of incubation, the enterobacteriaceae drug-sensitive plates were removed for separate use, and 10 μ L of the prepared 0.5mg/ml solution of Resazurin sodium was added to each test well and incubation continued for 60 min.
4) The enterobacteriaceae drug sensitive plate can be taken out for use respectively: 1) and (4) reading the result by visual inspection, wherein if the color of the indicator is red, pink or purple, the indicator represents the growth of microorganisms in the drug sensitivity test, and the antibacterial drug does not work. If the indicator is blue, bluish purple or purple, it means that the microorganism does not grow in the drug sensitive test, and the antibacterial drug acts. 2) And (3) judging the result of the fluorescence signal: exciting with 530nm exciting light, collecting 590nm fluorescence signal, comparing the collected signal with the negative control hole and the positive signal hole signal, if the test hole signal value is larger than (negative hole signal + positive hole signal)/2, the test hole is judged to be positive, otherwise, the test hole is judged to be negative.
Example 3
1) 5.0mg of the Resazurin sodium salt is weighed and added with purified water to prepare a solution of 5mg/ml, and the solution is filtered by a sterile filter membrane of 0.22 mu m for later use.
2) Penicillin and amikacin were weighed at 5mg each and made up into a 5mg/ml solution with sterile purified water.
3) Two groups of sterile test tubes are respectively numbered A, B, each group has 12 tubes, 4ml of sterile purified water is added into the first tube of each group, and 2ml of sterile purified water is added into each of the other tubes.
4) 5mg/ml of the solution of the Resazurin sodium salt was added to the tubes of groups A and B, wherein the 5mg/ml solution of the Resazurin sodium salt was added in 40. mu.L in 2ml tubes and 80. mu.L in 4ml tubes.
5) To the first tube of group A, 25.6. mu.L of the prepared 5mg/ml penicillin solution was added, and then diluted in duplicate into the next 9 tubes, numbered A1, A2, A3, A4, A5, A6, A7, A8, A9 and A10, respectively, and the remaining two tubes not diluted in duplicate were numbered A11 and A12, respectively.
6) 25.6 μ L of the prepared 5mg/ml amikacin solution was added to the first tube of group B tubes, and then diluted in multiple to the next 9 tubes, numbered B1, B2, B3, B4, B5, B6, B7, B8, B9, and B10, respectively. The remaining two tubes not diluted in multiple were numbered B11 and B12, respectively.
7) 2 pieces of 96-well plate were taken and group A reagents were dispensed among them, and 1 to 12 columns were dispensed with liquids in A1 to A12 test tubes, respectively. The slats are numbered A-1 and A-2.
8) Taking 2 pieces of 96-well plates, and subpackaging the group B reagents into the plates, wherein 1 to 12 columns are respectively subpackaged with the liquid in the test tubes B1 to B12. The slats are numbered B-1 and B-2.
9) Drying A-1, A-2, B-1 and B-2.
10) The dried drug-sensitive strip was removed and 100. mu.L of bacteria (ATCC29213 Staphylococcus aureus) in an amount of about 1X 10 was added to each of the 1-11 rows5The drug sensitive inoculation culture solution. Adding 100 μ L of drug sensitive inoculation culture solution containing no microorganism into column 12, wherein columns 1-10 are drug sensitive test hole sites, and column 11 is positive controlWells, 12 th column is negative control well.
11) The plates were incubated at 35 ℃ for 20 h.
12) The panels were removed and used separately: 1) and (4) reading the result by visual inspection, wherein if the color of the indicator is red, pink or purple, the indicator represents the growth of microorganisms in the drug sensitivity test, and the antibacterial drug does not work. If the indicator is blue, bluish purple or purple, it means that the microorganism does not grow in the drug sensitive test, and the antibacterial drug acts. 2) And (3) judging the result of the fluorescence signal: exciting with 530nm exciting light, collecting 590nm fluorescence signal, comparing the collected signal with the negative control hole and the positive signal hole signal, if the test hole signal value is larger than (negative hole signal + positive hole signal)/2, the test hole is judged to be positive, otherwise, the test hole is judged to be negative.
13) Each of the two antibacterial drugs contained 16 MIC value results (penicillin: the total number of the medicines on the A-1 and the A-2 is 16, each medicine can output an MIC value, and 16 MIC value results are obtained; and in the same way, amikacin also obtains 16 MIC value results), the 16 test results of each antibacterial drug are consistent, and are respectively shown in Table 1, and Table 2 shows the subpackaging condition of the A-1 plates.
TABLE 1 test results
| Antibacterial agent | Visual reading of MIC values | Reading MIC value of fluorescent signal |
| Penicillin | 1 | 1 |
| Amikacin | 2 | 2 |
TABLE 2A-1 plate racking conditions
The embodiments described above are described to facilitate an understanding and use of the invention by those skilled in the art. It will be readily apparent to those skilled in the art that various modifications to these embodiments may be made, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above embodiments, and those skilled in the art should make improvements and modifications within the scope of the present invention based on the disclosure of the present invention.
Claims (9)
1. A method for testing drug sensitivity of microorganisms by using a drug sensitivity indicator is characterized in that resazurin or soluble resazurin salts thereof are used as the drug sensitivity indicator, and the result of the drug sensitivity test of the microorganisms is indicated by color and/or fluorescence signals.
2. The method for testing the susceptibility test of microorganisms by using the drug susceptibility indicator according to claim 1, wherein the result of the drug susceptibility test is indicated by color, specifically, if the color of the indicator is red, pink or purple, it represents the growth of microorganisms in the drug susceptibility test, and the antibacterial agent does not work;
if the indicator is blue, bluish purple or purple, it means that the microorganism does not grow in the drug sensitive test, and the antibacterial drug acts.
3. The method of claim 1, wherein the fluorescence signal is used to indicate the result of the drug sensitivity test, and the indicator is irradiated with excitation light, and if a strong fluorescence signal appears, the indicator represents the growth of the microorganism in the drug sensitivity test, and the antibacterial agent does not act, and if no strong fluorescence signal appears, the indicator represents the growth of the microorganism in the drug sensitivity test, and the antibacterial agent acts.
4. The method for detecting the microbial susceptibility test by using the susceptibility indicator according to claim 3, wherein the excitation light has a wavelength of 500-540 nm, and the fluorescence signal having a wavelength of 570-620 nm is collected.
5. The method as claimed in claim 1, wherein the resazurin or its soluble salt is coated in advance as one of the components of the drug sensitive reagent or added as an independent reagent during the drug sensitive test.
6. The method for testing the microbial susceptibility test by using the drug susceptibility indicator according to claim 5, wherein the resazurin or the soluble resazurin salt thereof is coated into the drug susceptibility reagent in advance to become one of the components of the drug susceptibility reagent by directly adding the resazurin or the soluble resazurin salt into the drug susceptibility reagent to become one of the components of the drug susceptibility plate/card/disc.
7. The method for testing the microbial susceptibility test by using the susceptibility indicator of claim 5, wherein the resazurin or the soluble resazurin salt thereof is used as an independent reagent,
when a drug susceptibility test is carried out, resazurin or soluble resazurin salts are prepared into a solution and added into a drug susceptibility inoculation culture solution;
or in the drug susceptibility test culture stage, preparing the resazurin or soluble resazurin salts into a solution, and adding the solution into a drug susceptibility test reaction hole;
or preparing solution of resazurin or soluble resazurin salt 15 min-2 h before the result of the drug sensitivity test is judged, and adding the solution into the reaction hole of the drug sensitivity test.
8. A method according to claim 6 or 7, wherein the resazurin or the soluble salt thereof is added in an amount to ensure a concentration of 5 μ g/mL to 100 μ g/mL in the susceptibility test.
9. An assay method according to claim 1 for carrying out a susceptibility test on a microorganism, including but not limited to bacteria and/or fungi, using a susceptibility indicator.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102199653A (en) * | 2010-03-28 | 2011-09-28 | 海南大学 | Method for rapidly screening and evaluating antibiotic bioactivator |
| CN102559844A (en) * | 2012-01-09 | 2012-07-11 | 上海交通大学 | Preparation method of detecting system for minimal inhibitory concentration of mycobacteria |
| CN103013822A (en) * | 2012-12-24 | 2013-04-03 | 山东省海水养殖研究所 | Fast drug sensitivity detection kit for aquatic product vibrio |
| CN108866152A (en) * | 2018-06-06 | 2018-11-23 | 苏州艾可瑞动物检测技术服务有限公司 | A kind of high-throughput quick medicine-sensitive detection kit used for veterinary clinic |
-
2019
- 2019-12-29 CN CN201911385575.9A patent/CN110938672A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102199653A (en) * | 2010-03-28 | 2011-09-28 | 海南大学 | Method for rapidly screening and evaluating antibiotic bioactivator |
| CN102559844A (en) * | 2012-01-09 | 2012-07-11 | 上海交通大学 | Preparation method of detecting system for minimal inhibitory concentration of mycobacteria |
| CN103013822A (en) * | 2012-12-24 | 2013-04-03 | 山东省海水养殖研究所 | Fast drug sensitivity detection kit for aquatic product vibrio |
| CN108866152A (en) * | 2018-06-06 | 2018-11-23 | 苏州艾可瑞动物检测技术服务有限公司 | A kind of high-throughput quick medicine-sensitive detection kit used for veterinary clinic |
Non-Patent Citations (3)
| Title |
|---|
| 张均田等: "现代药理学实验方法（上、下）", vol. 2, pages: 1015 * |
| 王敏;付光宇;罗江卫;: "刃天青显色法检测结核分枝杆菌的耐药性", vol. 9, no. 13, pages 1588 * |
| 郭小玲;黄丽;李红;许瑞;: "活性菌检测方法研究进展", no. 29, pages 12 - 13 * |
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