CN110938531A

CN110938531A - Linked needle tube type micro-biological identification micro-biochemical tube

Info

Publication number: CN110938531A
Application number: CN201911364532.2A
Authority: CN
Inventors: 黄荔丰
Original assignee: Zhangzhou Health Vocational College
Current assignee: Zhangzhou Health Vocational College
Priority date: 2019-05-23
Filing date: 2019-12-26
Publication date: 2020-03-31
Also published as: CN110042049A

Abstract

The invention provides a row needle tube type micro-biological identification micro-biochemical tube, which is based on the structure of a multi-channel liquid-transferring gun and designs a multi-tube body-row type sampling structure. Specifically, the biochemical substrate is accommodated in the tube body made of plastic, the lower end of the tube body is connected with the needle head for absorbing bacteria liquid to be detected, and the side wall of the tube body is respectively provided with the label and the negative and positive color cards, so that the color development result can be compared more intuitively; the sampling structure saves the complex operation of repeated sampling of a single biochemical tube for many times, can ensure that the sampling amount of each tube is equal, and is convenient for transverse comparison of results. On the basis, the sealing sleeve and the sealing strip which are matched with each other are designed for the tube body, the tube body is accommodated in the groove body of the sealing sleeve, the lower end of the tube body and the needle head can be sealed relatively, and the outer wall adhered with bacteria liquid is prevented from being exposed in the air to cause pollution. The invention can obviously improve the efficiency of microorganism identification experiments and has good use effect.

Description

Linked needle tube type micro-biological identification micro-biochemical tube

Technical Field

The invention relates to the technical field of microorganism experimental equipment, in particular to a parallel needle tube type microorganism identification trace biochemical tube.

Background

The biochemical micro-tube is a test consumable used in the bacteria identification process, and has wide application in the microbial laboratory of the basic clinical laboratory and the microbial test course of medical institutions. Since different microorganisms have different enzyme systems, the ability to decompose substrates during metabolism varies, thereby producing different metabolites; based on this principle, a biochemical micro tube containing a biochemical substrate and a chromogenic component is used for culturing microorganisms, and microorganism identification can be achieved according to the chromogenic result of the culture.

The conventional trace biochemical tube is a single closed glass tube structure, and in a microorganism identification experiment, an inoculation link needs to strictly avoid mixed bacteria pollution, so that the closed glass tube is cut off by a sand wheel for standby in each inoculation, then an inoculation needle is used for flame sterilization, cooling and temperature testing, microorganisms are picked up, the tube orifice of the broken biochemical microtube is used for flame sterilization, then the inoculation needle for picking up the microorganisms is repeatedly ground on the inner wall of the trace biochemical tube, after the operation is finished, the tube orifice of the trace biochemical tube and the inoculation needle are used for flame sterilization again, and a group of experiment operation is finished after dozens of times of repetition, so that the efficiency is low. Under the condition, how to realize the simultaneous inoculation of a plurality of trace biochemical tubes on the basis of fully avoiding the mixed bacteria becomes a technical problem to be solved urgently; moreover, the contact chance between the sample and the air and between the sample and the operator should be minimized in the process, so as to avoid the invasion of the microorganism to the environment and the operator.

In addition, in the use process of the conventional trace biochemical tube, the inoculated bacterial quantity is random, the quantification is not easy, and the difference of experimental results is easy to cause. Moreover, each used tiny biochemical tube is in an open state, so that a biochemical substrate mixed with bacterial liquid can easily overflow to cause pollution, the tiny biochemical tubes which are randomly placed need to be arranged according to smooth arrangement again when recording test results, then the color development results of the tiny biochemical tubes are compared with the specification, the biochemical results are converted into numerical codes, and identification results are retrieved through codes.

Disclosure of Invention

The invention aims to provide a row needle tube type microbial identification micro biochemical tube aiming at the technical defects in the prior art, and aims to solve the technical problem that a plurality of micro biochemical tubes are difficult to realize simultaneous inoculation on the premise of ensuring sterile operation in the prior art.

The invention also aims to solve the technical problem that in the inoculation process, after the outer wall of the micro biochemical tube needle contacts with a sample, the micro biochemical tube needle is exposed in the air, so that the micro biochemical tube needle has higher damage risk to the environment and operators.

The invention also aims to solve the technical problem that the inoculated bacterial quantity is difficult to quantify in the using process of the conventional trace biochemical tube.

The invention also aims to solve the technical problem that the detection result of the conventional trace biochemical tube needs to be searched and determined by turning over the specification and is not intuitive.

In order to achieve the technical purpose, the invention adopts the following technical scheme:

a row needle tube type micro-biochemical tube for microorganism identification comprises a tube body, a lower conical surface, a needle head, an inclined surface, a label, a positive color card, a negative color card, a biochemical substrate, a sealing sleeve, a groove body, an upper conical surface, a needle channel, a sealing strip, a pipetting gun and a connecting piece, wherein the lower end of the tube body is provided with the lower conical surface, the lower end of the tube body is connected with the needle head, the lower end of the needle head is provided with the inclined surface, the upper end of the side wall of the tube body is attached with the label (used for marking a test item), the middle part of the side wall of the tube body is respectively attached with the positive color card and the negative; the upper end of seal cover has been seted up a plurality of cell bodies, has the last conical surface in the cell body, go up the conical surface with the conical surface cooperatees down, and the cell body cooperatees with the lower extreme of body (above-mentioned two kinds of cooperation relations play fixed action jointly), in the seal cover, lie in the position of cell body lower extreme and seted up the needle passageway, is connected with the sealing strip at the lower extreme of seal cover, the lower extreme of needle passageway is sealed to the sealing strip, and the port department at the liquid-transfering gun is pegged graft to the upper end of body, the body has a plurality of, a plurality of body is arranged.

Preferably, the device further comprises a bacteria liquid groove, bacteria liquid is contained in the bacteria liquid groove, and the lower end of the needle head is immersed in the bacteria liquid.

Preferably, the lower end of the tube body is inserted in the groove body, the needle head is inserted in the needle channel, and the inclined surface is wedged in the sealing strip.

Preferably, the material of the sealing strip is rubber or foam plastic.

Preferably, a sealing cap is sleeved at the upper port of the pipe body.

Preferably, the upper end of the pipe body is in a shape of a circle, a triangle, a hexagon, a square or a rectangle.

In the technical scheme, the tube body is made of plastic and is used as a mixing place of the bacterial liquid and the biochemical substrate, and the color development process is completed in the tube body; the lower conical surface of the pipe body is matched with the upper conical surface of the groove body of the sealing strip, so that the pipe body is stably inserted into the groove body, and meanwhile, the conical surface structure can play a role in guiding insertion; the needle head at the lower end of the tube body is used for sucking bacteria liquid to be detected; the inclined surface is more convenient for wedging the needle head into the sealing strip; the label is used for marking the test item; the positive color card and the negative color card are convenient for visually comparing the color development results; the biochemical substrate is a conventional reagent used in a strain identification experiment, the specific category and the dosage can be determined according to the general technical knowledge in the field of microorganisms, the biochemical substrate is generally solid, and the solid biochemical substrate cannot leak from a needle head because the diameter of the needle head is extremely small; the sealing sleeve is used for sealing the positions of the tube body and the lower part of the needle head, which are contacted with bacteria liquid, so that the environmental pollution and the human body invasion caused by the exposure of the tube body and the lower part of the needle head in the air are avoided as much as possible; in the specific structure of the sealing sleeve, the groove body is used for inserting and fixing the pipe body; the upper conical surface of the groove body of the sealing sleeve is matched with the lower conical surface of the pipe body, and the sealing sleeve plays a role in guiding when inserted; the needle passage is used for accommodating and sealing the needle head therein; the sealing strip is made of rubber or foamed plastic and used for sealing the needle channel and the needle head, and the sealing strip can be fixedly connected with the sealing sleeve or can be of a split structure; the liquid transferring gun is used for providing power for absorbing bacterial liquid; the bacterium liquid groove is used for containing bacterium liquid to be measured to a set of body is soaked among them side by side for absorbing the bacterium liquid. The sealing cap at the upper end of the tube body can be any structure which can play a sealing role, and comprises but is not limited to a plastic packaging film, a plastic end cover and the like.

When the device is used, the sealing cap at the upper end of the tube body is unsealed, the upper port of the tube body is connected to a liquid transfer gun, the needle head is immersed in the bacteria liquid tank, and the bacteria liquid is absorbed by the liquid transfer gun; after the bacteria liquid is sucked into the tube body, the tube body is inserted into the groove body of the sealing sleeve side by side, so that the needle head is accommodated in the needle channel, and the inclined surface is kept inserted into the sealing strip; separating the pipette from the tubes in the row, sealing the upper ends of the tubes by using the sealing caps again, and then standing for reaction; in the tube body, the bacterial liquid is mixed with a biochemical substrate, color development is carried out, and the identification result is obtained by comparing the color development condition with a color card.

The invention provides a row needle tube type micro-biological identification micro-biochemical tube, which is based on the structure of a multi-channel liquid-transferring gun and designs a multi-tube body-row type sampling structure. Specifically, the biochemical substrate is accommodated in the tube body made of plastic, the lower end of the tube body is connected with the needle head for absorbing bacteria liquid to be detected, and the side wall of the tube body is respectively provided with the label and the negative and positive color cards, so that the color development result can be compared more intuitively; the sampling structure saves the complex operation of repeated sampling of a single biochemical tube for many times, can ensure that the sampling amount of each tube is equal, and is convenient for transverse comparison of results. On the basis, the sealing sleeve and the sealing strip which are matched with each other are designed for the tube body, the tube body is accommodated in the groove body of the sealing sleeve, the lower end of the tube body and the needle head can be sealed relatively, and the outer wall of the needle head which is adhered with bacteria liquid is prevented from being exposed in the air to cause pollution. The invention can obviously improve the efficiency of microorganism identification experiments and has good use effect.

Drawings

FIG. 1 is a view showing a state in which a tube and a needle are inserted into a sealing sleeve according to the present invention;

figure 2 is a schematic view of the construction of the sealing sleeve according to the invention;

FIG. 3 is a schematic view of the tube and needle of the present invention;

FIG. 4 is a schematic view of the structure of a bacteria liquid tank according to the present invention;

FIG. 5 is a view showing the tube and needle of the present invention mounted to a pipette;

in the figure:

1. tube body 2, lower conical surface 3, needle 4 and inclined plane

5. Label 6, positive color card 7, negative color card 8, biochemical substrate

9. Sealing sleeve 10, groove body 11, upper conical surface 12 and needle channel

13. Sealing strip 14, pipette 15, fungus liquid groove 16, connection piece.

Detailed Description

Hereinafter, specific embodiments of the present invention will be described in detail. Well-known structures or functions may not be described in detail in the following embodiments in order to avoid unnecessarily obscuring the details. Approximating language, as used herein in the following examples, may be applied to identify quantitative representations that could permissibly vary in number without resulting in a change in the basic function. Unless defined otherwise, technical and scientific terms used in the following examples have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

A row of needle tube type micro-biochemical tube for microorganism identification is shown in figures 2, 3 and 5 and comprises a tube body 1, a lower conical surface 2, a needle 3, an inclined surface 4, a label 5, a positive color card 6, a negative color card 7, a biochemical substrate 8, a sealing sleeve 9, a groove body 10, an upper conical surface 11, a needle channel 12, a sealing strip 13, a liquid transferring gun 14 and a connecting sheet 16, wherein the lower conical surface 2 is arranged at the lower end of the tube body 1, the needle 3 is connected to the lower end of the tube body 1, the inclined surface 4 is arranged at the lower end of the needle 3, the label 5 is attached to the upper end of the side wall of the tube body 1, the positive color card 6 and the negative color card 7 are respectively attached to the middle part of the side wall of the tube body 1, and the biochemical substrate; a plurality of cell bodies 10 have been seted up to the upper end of seal cover 9, have in cell body 10 the conical surface 11, go up the conical surface 11 with the conical surface 2 cooperatees down, and cell body 10 cooperatees with the lower extreme of body 1, and in seal cover 9, the position that is located the cell body 10 lower extreme has seted up needle passageway 12, is connected with sealing strip 13 at the lower extreme of seal cover 9, the lower extreme of needle passageway 12 is sealed to sealing strip 13, and the port department at pipetting gun 14 is pegged graft to the upper end of body 1, body 1 has a plurality of, a plurality of body 1 arranges side by side, fixedly connected with connection piece 16 between the adjacent body.

Meanwhile, as shown in fig. 4, the device further comprises a bacteria liquid tank 15, wherein bacteria liquid is contained in the bacteria liquid tank 15, and the lower end of the needle 3 is immersed in the bacteria liquid. As shown in fig. 1, the lower end of the tube body 1 is inserted into the groove body 10, the needle head 3 is inserted into the needle passage 12, and the inclined surface 4 is wedged into the sealing strip 13. The sealing strip 13 is made of rubber or foam plastic. When the pipe body 1 is not used, a sealing cap is sleeved at the upper port of the pipe body 1; before use, the sealing cap is unsealed and then used. The shape of the upper port of the pipe body 1 can be selected from round, triangular, hexagonal, square or rectangular.

The working principle of the device is as follows: the tube body 1 is made of plastic materials, and the tube body 1 is used as a mixing place of bacterial liquid and biochemical substrates, and the color development process is completed in the tube body 1; the lower conical surface 2 of the pipe body 1 is matched with the upper conical surface 11 of the groove body 10 and is used for stably inserting the pipe body 1 into the groove body 10, and meanwhile, the conical surface structure can play a role in guiding insertion; the needle 3 at the lower end of the tube body 1 is used for sucking bacteria liquid to be detected; the bevel 4 further facilitates wedging of the needle 3 into the sealing strip 13; the label 5 is used for marking the test item number; the positive color card 6 and the negative color card 7 are convenient for visually comparing the color development results; the biochemical substrate 8 is a conventional reagent used in a strain identification experiment, the specific category and the dosage can be determined according to the general technical knowledge in the field of microorganisms, the biochemical substrate 8 is generally solid, and the solid biochemical substrate 8 cannot leak from the needle 3 because the diameter of the needle 3 is extremely small; the sealing sleeve 9 is used for sealing the positions of the lower parts of the tube body 1 and the needle head 3, which are contacted with bacteria liquid, so that the environmental pollution and the human body invasion caused by the exposure of the tube body and the needle head in the air are avoided as much as possible; in the specific structure of the sealing sleeve 9, the groove body 10 is used for inserting and fixing the pipe body 1; the upper conical surface 11 is matched with the lower conical surface 2 of the tube body 1 and plays a role in guiding when inserted; the needle passage 12 is used for accommodating and sealing the needle head 3 therein; the sealing strip 13 is made of rubber or foamed plastic and used for sealing the needle channel 12 and the needle head 3, and the sealing strip 13 can be fixedly connected with the sealing sleeve 9 or can be of a split structure; the liquid transferring gun 14 is used for providing power for absorbing bacteria liquid; the bacteria liquid tank 15 is used for containing bacteria liquid to be measured so that a group of tube bodies 1 can be immersed in the bacteria liquid side by side to absorb the bacteria liquid.

When in use, the sealing cap at the upper end of the tube body 1 is unsealed, the upper port of the tube body 1 is connected to the pipette gun 14, the needle 3 is immersed in the bacteria liquid groove 15, and the bacteria liquid is sucked by the pipette gun 14; after the bacteria liquid is sucked into the tube body 1, the tube body 1 is inserted into the groove body 10 of the sealing sleeve 9 side by side, so that the needle head 3 is accommodated in the needle channel 12, and the inclined plane 4 is kept inserted into the sealing strip 13; separating the pipette 14 from the tubes 1 in the row, sealing the upper ends of the tubes 1 by using the sealing caps again, and then standing for reaction; in the tube body 1, the bacterial liquid is mixed with a biochemical substrate 8, color development is carried out, and comparison with a color card is carried out according to the color development condition to obtain an identification result.

The beneficial effects of the technical scheme are reflected in the following aspects:

1. the method is characterized in that 0.5 McLeod suspension is prepared in advance, prepared bacteria liquid is guided into a bacteria liquid groove which is matched with the bacteria liquid groove, the linked needle tube type microorganism identification biochemical micro-tube is connected with a micro multi-channel pipette, and inoculation of various biochemical micro-tubes can be completed at one time by pressing a liquid suction switch of the micro multi-channel pipette, so that the process of repeatedly performing flame disinfection and sterilization on a test tube opening and an inoculation needle of the biochemical micro-tube is omitted, and the operation efficiency is improved.

2. The operation mode of inoculating 0.5 McLeeb turbidimetric bacteria suspension to the row needle tube type microorganism identification biochemical microtubes is completed by pressing the liquid suction switch of the miniature multi-channel pipette, so that the same bacterial liquid amount and relatively uniform bacterial amount can be ensured to be inoculated in each biochemical microtube.

3. The complicated step of arranging different series of biochemical micro tubes according to the specified sequence is omitted when the biochemical reaction result is observed, and the efficiency of observing and recording the result is improved.

4. Each tube of the row needle tube type microorganism identification biochemical microtube is stuck or printed with a positive color block and a negative color block corresponding to each biochemical test and a numerical code corresponding to the positive biochemical test of each tube, thereby avoiding the inconvenience of searching a specification when observing and recording test results.

5. When the row needle tube type microorganism identification biochemical micro-tube is used, the biochemical micro-tube does not need to be turned frequently to search a certain needed biochemical micro-tube, biochemical reaction is carried out in a relatively sealed space, bacterial liquid is not easy to leak, environment pollution is not easy to cause, harm is caused to operators, and biological safety protection is facilitated.

Before inoculation, the configuration method of the gang needle tube type microorganism identification biochemical micro-tube is that under the aseptic environment, sterile biochemical dry powder required by different types of bacteria identification is placed in different tubes of the gang needle tube type microorganism identification biochemical micro-tube, then the needle head end is sealed by a sterile needle head sealing sleeve, the rear end is bonded and sealed by a sterile plastic film or sealed by a sealing cover, the biochemical dry powder placed in the gang needle tube type microorganism identification biochemical micro-tube is prevented from being affected with damp, the gang needle tube type microorganism identification biochemical micro-tube is disposable, and the gang needle tube type microorganism identification biochemical micro-tube can be discarded after being used up.

When the combined type needle liquid tank is used, pure bacterial colonies to be identified are firstly mixed into 0.5 McLeod ratio turbid bacterial suspension and placed in a matched bacterial liquid tank for standby, the volume of bacterial liquid capable of being placed in the bacterial liquid tank must meet the total bacterial liquid quantity required by the combined type needle tube type microorganism identification biochemical micro-tube, and the length of the bacterial liquid tank must ensure that all needles of the combined type needle tube type microorganism identification biochemical micro-tube can be placed.

When inoculating, tearing off a sealing strip at the rear end, or taking off a sealing cover, connecting the row needle tube type microorganism identification biochemical micro-tube with the micro multi-channel pipette, then pressing a liquid suction switch of the micro multi-channel pipette, carefully sucking bacteria liquid with a certain scale to finish the operation, in order to avoid overlarge bacteria liquid suction amount, setting a volume which can be sucked by the micro multi-channel pipette in advance, namely avoiding that the result observation is influenced by insufficient liquid suction amount and incapability of filling all micro-tubes; and also prevent the pollution caused by the fact that the bacteria liquid is sucked into the miniature multi-channel liquid-transferring gun due to too large liquid-sucking amount.

Because the specification of each tube of the linked needle tube type microorganism identification biochemical microtube is the same, the volume of the bacterial liquid sucked by each tube when the liquid suction switch of the miniature multi-channel pipette is pressed is also the same, and the concentration of the sucked bacterial liquid is 0.5 McLeeb turbidimetric prepared in advance, the relatively uniform number of the bacteria inoculated in each tube is ensured. After inoculation, the micro multi-channel pipette can be directly pulled out and then connected with other lined needle tube type microorganism identification biochemical micro tubes, and the micro multi-channel pipette can be reused under the condition of no pollution.

The needle head end of the interlinked row needle tube type microorganism identification biochemical micro-tube inoculated with the bacterial liquid needs to be sleeved with the needle head sealing sleeve again, and the rear end of the interlinked row needle tube type microorganism identification biochemical micro-tube needs to be adhered with a sealing strip again or covered with a sealing cover again. Then, the cells were incubated at 37 ℃.

When the aligned needle tube type microorganism identification biochemical microtubes cultured in an incubator at 37 ℃ for 24 hours were taken out, it was found that each tube exhibited various colors.

And then recording the experimental result of each micro tube according to the color provided by the positive color block and the negative color block pasted and printed on each tube as a reference, expressing the positive and negative results by corresponding numerical values, generally listing three experiments as a group, and adding the numerical values to obtain a string of numerical codes.

And searching the numerical code to obtain a corresponding bacteria identification result.

The embodiments of the present invention have been described in detail, but the description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention. Any modification, equivalent replacement, and improvement made within the scope of the application of the present invention should be included in the protection scope of the present invention.

Claims

1. A row-needle-tube type micro biochemical tube for microorganism identification is characterized by comprising a tube body (1), a lower conical surface (2), a needle (3), an inclined surface (4), a label (5), a positive color card (6), a negative color card (7), a biochemical substrate (8), a sealing sleeve (9), a groove body (10), an upper conical surface (11), a needle channel (12), a sealing strip (13), a liquid transferring gun (14) and a connecting sheet (16), wherein, the lower end of the tube body (1) is provided with a lower conical surface (2), the lower end of the tube body (1) is connected with a needle head (3), the lower end of the needle head (3) is provided with an inclined surface (4), a label (5) is attached to the upper end of the side wall of the tube body (1), a positive color card (6) and a negative color card (7) are respectively attached to the middle part of the side wall of the tube body (1), and a biochemical substrate (8) is contained in the tube body (1); a plurality of groove bodies (10) have been seted up to the upper end of seal cover (9), have in groove body (10) and go up conical surface (11), go up conical surface (11) with lower conical surface (2) cooperate, and groove body (10) cooperate with the lower extreme of body (1), in seal cover (9), the position that is located groove body (10) lower extreme has seted up needle passageway (12), is connected with sealing strip (13) at the lower extreme of seal cover (9), the lower extreme of needle passageway (12) is sealed in sealing strip (13), and the port department at pipetting gun (14) is pegged graft to the upper end of body (1), body (1) has a plurality of, a plurality of body (1) are arranged side by side, fixedly connected with connection piece (16) between adjacent body (1).

2. The inline needle type microbiology identification micro biochemical tube according to claim 1, further comprising a bacteria solution tank (15), wherein bacteria solution is contained in the bacteria solution tank (15), and the lower end of the needle (3) is immersed in the bacteria solution.

3. The aligned needle tube type micro biochemical tube for microorganism identification according to claim 1, wherein the lower end of the tube body (1) is inserted into the groove body (10), the needle (3) is inserted into the needle channel (12), and the inclined surface (4) is wedged into the sealing strip (13).

4. The aligned needle type micro biochemical tube for microorganism identification according to claim 1, wherein the material of the sealing strip (13) is rubber or foam plastic.

5. The aligned needle type micro biochemical tube for microorganism identification according to claim 1, wherein a sealing cap is sleeved at the upper port of the tube body (1).

6. The aligned needle type micro biochemical tube for microorganism identification according to claim 1, wherein the shape of the upper port of the tube body (1) is circular, triangular, hexagonal, square or rectangular.