CN212504878U - TIP head type bacterium quantitative inoculating loop - Google Patents
TIP head type bacterium quantitative inoculating loop Download PDFInfo
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- CN212504878U CN212504878U CN202020825874.1U CN202020825874U CN212504878U CN 212504878 U CN212504878 U CN 212504878U CN 202020825874 U CN202020825874 U CN 202020825874U CN 212504878 U CN212504878 U CN 212504878U
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Abstract
The utility model discloses a TIP head type bacteria quantitative inoculating loop, which comprises a bacterial colony picking loop, a bacterial colony picking cavity and a bacterial colony inoculating loop, wherein the bacterial colony picking cavity is used for picking bacterial colonies to be inoculated which are contained in an agar plate; the upper part of the liquid-transfering gun connecting part is a cylindrical connecting sleeve which is in adaptive connection with the handheld liquid-transfering gun, and the lower part of the liquid-transfering gun connecting part is an inverted cone-shaped air-blowing channel which is in sealed communication with the lower end of the cylindrical connecting sleeve; wherein, the lower extreme of back taper gas blowing passageway links firmly with the outer wall of colony picking ring mutually, sets up the through-hole of intercommunication picking chamber and the inner chamber of back taper gas blowing passageway at its junction. The utility model has the advantages of simple structure, simple operation, small volume and low cost; the device can be adapted to the existing hand-held pipette or a full-automatic microorganism inoculation instrument, so that the inoculation operation is simpler, more convenient, quicker and more efficient, and the contact between the hands and microorganisms is avoided; the blowing, beating and uniformly mixing of bacterial colonies can be completed by means of a handheld pipette or a full-automatic microorganism inoculation instrument, so that the uniformity of uniform mixing is better.
Description
Technical Field
The utility model belongs to the technical field of the microbiological examination technique and specifically relates to a can with first type bacterium ration transfering loop of TIP of hand-held type pipetting gun looks adaptation.
Background
Micro-organisms, a large group of organisms including bacteria, viruses, fungi, and some small protists, microscopic algae, etc.; for human beings, some microorganisms which can cause infection and even death of human body under certain conditions are called pathogenic microorganisms, and are very closely related to human beings.
With the gradual and deep research on pathogenic microorganisms, people have more deep understanding on the pathogenicity, in vitro culture and drug sensitivity characteristics of the pathogenic microorganisms; in the current clinical testing of microorganisms, the tester needs to perform quantitative inoculation on the microorganisms in some cases, such as: when microorganism identification and microorganism in-vitro drug sensitivity monitoring tests are carried out, the using amount of microorganisms needs to be controlled so as to obtain more accurate identification or drug sensitivity results, and thus, the clinical diagnosis and treatment of microorganism infection diseases are more effectively carried out.
In the existing field of microbiological testing, the common means for inspectors to achieve bacterial load control are through optical instruments such as: and a turbidimeter for measuring the OD value of the bacterial liquid by ultraviolet light so as to adjust the concentration of the bacterial strain. In addition, there are some quantitative inoculating needles or quantitative inoculating loops for controlling bacterial amount in the market, such as: the application date is 17.4.2019, the novel quantitative bacterium maker with the patent number of 201920519663.2, or the application date is 25.4.2018, and the novel quantitative bacterium maker with the patent number of 201820647191.4 is a 'one-bacterium inoculating loop'. Although the novel quantitative bacteria maker can pick a certain number of bacterial colonies to meet the quantitative requirement, the novel quantitative bacteria maker has the disadvantages of large overall volume, high cost, inconvenience for flexible operation, and troublesome operation because the novel quantitative bacteria maker needs to perform mixing treatment by other equipment; and "a bacterium transfering loop" then need manual contact to use, and whole specification and size is great, can't realize automated operation, is not convenient for preserve in batches, and there is certain pollution probability transfering loop after unsealing to the design of this transfering loop is not quantified, can't predict the quantity of picking the bacterial colony, and the mixing process need rock with the help of the manual work, consumes artifical physical power great.
Disclosure of Invention
An object of the utility model is to provide a first type bacterium ration transfering loop of TIP, with hand-held type pipetting gun looks adaptation, can be convenient for dismouting, ration pick bacterial colony and mixing blow and beat.
In order to achieve the above purpose, the utility model can adopt the following technical proposal:
the TIP head type bacteria quantitative inoculating loop of the utility model comprises a bacterial colony picking loop which is provided with a picking cavity and is used for picking bacterial colonies to be inoculated which are contained in an agar plate; the upper part of the liquid-transfering gun connecting part is a cylindrical connecting sleeve which is in adaptive connection with the handheld liquid-transfering gun, and the lower part of the liquid-transfering gun connecting part is an inverted cone-shaped air-blowing channel which is in sealed communication with the lower end of the cylindrical connecting sleeve; the lower end of the inverted cone-shaped air blowing channel is fixedly connected with the outer wall of the bacterial colony picking ring, and a through hole communicated with the picking cavity and the inner cavity of the inverted cone-shaped air blowing channel is formed in the joint of the inverted cone-shaped air blowing channel and the bacterial colony picking ring.
The cylindrical connecting sleeve and the inverted cone-shaped air blowing channel are coaxially arranged, and the diameter of the lower end of the cylindrical connecting sleeve is consistent with that of the upper end of the inverted cone-shaped air blowing channel.
The axis of the colony picking ring is perpendicular to the axis of the inverted cone-shaped blowing channel.
The inner wall of the cylindrical connecting sleeve is a rough surface.
The utility model has the advantages of simple structure, easy and simple to handle, small, with low costs, it is convenient nimble and safe high-efficient to use more. The liquid-transferring gun connecting part is arranged on the colony picking ring to be matched with the conventional handheld liquid-transferring gun or full-automatic microorganism inoculation instrument, so that the inoculation operation is simpler, more convenient, quicker and more efficient, and after the bacterial colony picking ring is used, a gun head dismounting device matched with the handheld liquid-transferring gun or the full-automatic microorganism inoculation instrument can be adopted to automatically pick the colony picking ring, so that the contact between the hands and the microorganisms is effectively avoided, the biological safety is improved, and the foundation is laid for the automatic and streamlined operation of the microorganism inspection; the through hole formed at the joint of the inverted cone-shaped air blowing channel and the colony picking ring can be used for blowing, beating and uniformly mixing picked colonies to be inoculated by means of a handheld pipette or a full-automatic microorganism inoculation instrument, so that the colonies are uniformly mixed in a liquid medium; in addition, the inoculating loop can also be easily adapted to a carrying box for containing the TIP head, and batch sterilization treatment is easily realized.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a reference diagram of the usage state of the present invention.
Detailed Description
The embodiments of the present invention will be described in detail with reference to the accompanying drawings, which are implemented on the premise of the technical solution of the present invention, and the detailed embodiments and the specific operation procedures are given, but the scope of the present invention is not limited to the following embodiments.
As shown in fig. 1, the TIP type quantitative inoculating loop of the present invention comprises a colony picking loop 1 having a picking cavity 2 for picking a colony to be inoculated contained in an agar plate; the upper part of the liquid-transfering gun connecting part is a cylindrical connecting sleeve 3, and the lower part of the liquid-transfering gun connecting part is an inverted cone-shaped air-blowing channel 4 which is hermetically communicated with the lower end of the cylindrical connecting sleeve 3; wherein, the lower extreme of back taper air blowing passageway 4 links firmly with the outer wall that the ring 1 was chosen to the bacterial colony mutually, sets up the intercommunication in its junction and chooses the through-hole of the inner chamber of chamber 2 and back taper air blowing passageway 4, can easily realize through this through-hole that hand-held type liquid-transfering gun blows to choosing in the chamber 2.
In addition, the cylindrical connecting sleeve 3 and the inverted cone-shaped air blowing channel 4 are coaxially arranged, and the diameter of the lower end of the cylindrical connecting sleeve 3 is consistent with that of the upper end of the inverted cone-shaped air blowing channel 4, so that a structure which is the same as that of the existing TIP head is formed, and the connecting sleeve is adapted to an existing handheld pipette or a full-automatic microorganism inoculation instrument and is convenient to install; and can also adopt the rifle head dismounting device with hand-held type pipette or full-automatic microorganism inoculation instrument looks adaptation after finishing using to take off the bacterial colony automatically and choose ring 1, effectively avoid the contact of hand and microorganism, improve biological safety.
In order to conveniently pick bacterial colonies, the axis of the bacterial colony picking ring 1 is perpendicular to the axis of the inverted cone-shaped air blowing channel 4, namely the axis of the bacterial colony picking ring 1 is perpendicular to the axis of a handheld liquid transfer gun or a full-automatic microorganism inoculation instrument arranged on the cylindrical connecting sleeve 3, so that the operation is convenient, and the handheld liquid transfer gun or the full-automatic microorganism inoculation instrument can be guaranteed to blow air to the picking cavity 2; in order to further enhance the firmness of the connection with the handheld pipette or the full-automatic microorganism inoculation instrument, the inner wall of the cylindrical connecting sleeve 3 can be set to be a rough surface.
During actual processing, the colony picking ring 1 and the liquid transferring gun connecting part are of an integrated structure, the colony picking rings 1 with different sizes can be processed according to the selection of different thalli, for example, when staphylococcus aureus is selected, the colony picking ring 1 with a 1ul picking cavity can be adopted and inoculated into 2ml of normal saline for uniform mixing, and the average concentration of the obtained strain suspension is 1.12 x 108~8.53*108CFU/ml, total cell amount was measured, and the average was 2.24X 108~1.71*109CFU。
In practical use, as shown in fig. 2, the inoculating loop can be placed in a universal gun head carrying box for sterilization treatment before use in the same way as the TIP head, and then the front end of the hand-held liquid-transfering gun can be inserted into the cylindrical connecting sleeve 3; after the tight insertion is ensured, the handheld pipette gun can be lifted to carry out colony collection operation, so that the colony picking ring 1 is deep into a colony which is activated and well grown in an agar plate, and redundant colonies are scraped at the blank of the agar plate to reach a full ring; then, the colony picking ring 1 is deeply inserted into a bacteria liquid preparation medium, and a handheld liquid-transferring gun is operated to repeatedly blow air, so that the colonies are uniformly mixed in the liquid medium while the colonies in the colony picking ring 1 are blown out; after the preparation of the bacteria liquid is finished, the bacteria liquid can be taken down together with the bacterial colony picking ring 1 and the connecting part of the liquid transferring gun by using a gun head dismounting device matched with the handheld liquid transferring gun; when a plurality of strains are operated simultaneously, after the previous inoculating loop is taken down, another inoculating loop can be continuously loaded in the gun head carrying box for operation; the inoculating loop can also be matched with a full-automatic microorganism inoculating instrument for use, so that automation of inoculating operation of operation is realized, and a foundation is laid for realizing automation and streamline operation of microorganism detection.
Claims (4)
1. A TIP head type bacterium quantitative inoculating loop is characterized in that: comprises that
The colony picking ring is provided with a picking cavity and is used for picking the colony to be inoculated which is contained in the agar plate; and
the upper part of the liquid-transfering gun connecting part is a cylindrical connecting sleeve which is in adaptive connection with the handheld liquid-transfering gun, and the lower part of the liquid-transfering gun connecting part is an inverted cone-shaped air-blowing channel which is in sealed communication with the lower end of the cylindrical connecting sleeve;
the lower end of the inverted cone-shaped air blowing channel is fixedly connected with the outer wall of the bacterial colony picking ring, and a through hole communicated with the picking cavity and the inner cavity of the inverted cone-shaped air blowing channel is formed in the joint of the inverted cone-shaped air blowing channel and the bacterial colony picking ring.
2. The TIP head type bacterial quantitative inoculating loop of claim 1, which is characterized in that: the cylindrical connecting sleeve and the inverted cone-shaped air blowing channel are coaxially arranged, and the diameter of the lower end of the cylindrical connecting sleeve is consistent with that of the upper end of the inverted cone-shaped air blowing channel.
3. A TIP type bacteria quantification loop according to claim 1 or 2, characterized in that: the axis of the colony picking ring is perpendicular to the axis of the inverted cone-shaped blowing channel.
4. A TIP type bacteria quantification loop according to claim 1 or 2, characterized in that: the inner wall of the cylindrical connecting sleeve is a rough surface.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202020825874.1U CN212504878U (en) | 2020-05-18 | 2020-05-18 | TIP head type bacterium quantitative inoculating loop |
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| CN202020825874.1U CN212504878U (en) | 2020-05-18 | 2020-05-18 | TIP head type bacterium quantitative inoculating loop |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113234577A (en) * | 2021-04-14 | 2021-08-10 | 齐齐哈尔医学院 | Inoculating loop for microbial culture in microbial detection |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113234577A (en) * | 2021-04-14 | 2021-08-10 | 齐齐哈尔医学院 | Inoculating loop for microbial culture in microbial detection |
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