CN110928109A

CN110928109A - Culture dish shooting device and light source control method

Info

Publication number: CN110928109A
Application number: CN201911421980.1A
Authority: CN
Inventors: 厉刚; 刘科; 裴光辉; 王宇平; 谭军义; 石长林
Original assignee: Beijing Jun Kang Biotechnology Co Ltd
Current assignee: Beijing Jun Kang Biotechnology Co Ltd
Priority date: 2019-12-31
Filing date: 2019-12-31
Publication date: 2020-03-27

Abstract

The embodiment of the invention discloses a culture dish shooting device and a light source control method, wherein the culture dish shooting device comprises: the culture dish comprises a culture dish cover, a camera, an upper light source, a lower light source and an ultraviolet light source; the culture dish cover is disposable, is the tubbiness, and the back-off cover is established in the top of culture dish, forms relative closed environment with the culture dish, avoids the microbial contamination culture medium in the environment, and the culture medium is located the culture dish. The upper light source is arranged above the culture dish cover around the camera or arranged on the outer side of the culture dish cover; the lower light source is arranged below the culture dish; the ultraviolet light source is arranged on the outer side wall of the culture dish cover. The culture dish cover is covered above the culture dish, and the camera of the camera is arranged in the convex part at the bottom of the culture dish cover, so that the small culture dish shooting device is formed and is convenient to carry; meanwhile, the light sources at different positions are arranged, so that different light sources can be conveniently controlled to work according to different scenes, and bacterial colony images meeting different requirements can be acquired.

Description

Culture dish shooting device and light source control method

Technical Field

The invention relates to the technical field of microorganisms, in particular to a culture dish shooting device and a light source control method.

Background

In the microbiological examination of food, medicine, cosmetics, feed and the like, the items such as total bacterial colony number, coliform bacteria, escherichia coli, lactic acid bacteria and the like are involved, and a large amount of bacterial colony analysis and counting are needed in the statistical result. The colony is a single bacterium group which grows on the surface of a solid culture medium and can be seen by naked eyes after the bacterium is inoculated on the surface of the solid culture medium and cultured. Counting the number of colonies generated after sampling a target is a fundamental and important task in the related art. The colony image is the colony image formed by shooting the colony with a camera after the microorganism grows on the culture medium to form the colony, and the colony image has important significance for quantitative counting, type identification, microorganism growth and metabolism research of the microorganism.

At present, the colony image is mainly obtained through large-scale shooting equipment, and is inconvenient to carry; meanwhile, the accuracy of a colony counting result is influenced due to the fact that illumination is uneven, the obtained microbial colony image is not related to the growth environment condition, and the microbial colony form image in the uniform illumination environment cannot be formed through precise control over a light source.

Disclosure of Invention

Because the existing method has the problems, the embodiment of the invention provides a culture dish shooting device and a light source control method.

In a first aspect, an embodiment of the present invention provides a culture dish photographing device, including: the culture dish comprises a culture dish cover, a camera, an upper light source, a lower light source and an ultraviolet light source;

the culture dish cover is barrel-shaped, the upside-down cover is arranged above the culture dish, and the central part of the bottom surface of the culture dish cover is upwards protruded to form a protruded part;

the camera of the camera is arranged in the convex part and faces the culture dish;

the upper light source is arranged above the culture dish cover and is uniformly arranged around the camera, or is arranged outside the culture dish cover;

the lower light source is arranged at the lower position of the culture dish;

the ultraviolet light source is arranged on the outer side wall of the culture dish cover.

Optionally, the head of the camera is protruded from a protruded portion at the center of the bottom surface of the culture dish cover.

Optionally, the culture dish cover is hemispherical or barrel shaped.

Optionally, the culture dish photographing device further comprises: a communication unit and an information storage unit;

the communication unit is connected with the camera, receives the image shot by the camera and sends the image and the environment information when the image is shot;

the information storage unit is used for storing the image and the environment information when the image is shot.

Optionally, the camera of the camera is arranged above the culture dish for upright shooting, or the camera of the camera is arranged below the culture dish for inverted shooting.

Optionally, the culture dish photographing device further comprises a gyroscope;

the gyroscope is used for recording the upright culture state or the inverted culture state during shooting;

the upright culture state is a state in which the camera performs upright shooting, and the inverted culture state is a state in which the camera performs inverted shooting.

Optionally, the culture medium of the culture dish is arranged facing the camera, and the camera acquires a microorganism image in the culture medium after being irradiated by the upper light source;

or the like, or, alternatively,

the culture medium of culture dish sets up dorsad the camera, the camera passes through the little biological image in the culture medium is acquireed to the light source after shining.

Optionally, a plurality of the culture dish photographing devices are arranged in the same incubator.

Optionally, the upper light source and the lower light source are both formed into a uniform surface light source through a light homogenizing plate.

Optionally, the culture dish photographing device further comprises: a temperature and humidity sensor, a light intensity sensor and a gas partial pressure sensor;

temperature and humidity sensor, light intensity sensor and gaseous partial pressure sensor all locate the outside of culture dish cover is located in the shell of culture dish shooting device.

Optionally, the culture dish photographing device further comprises: a temperature and humidity alarm unit;

the temperature alarm unit is connected with the temperature and humidity sensor and alarms when the temperature and humidity are detected to deviate from a preset range.

In a second aspect, an embodiment of the present invention further provides a light source method for a culture dish photographing device, including:

receiving an image acquisition instruction sent by a terminal;

and controlling to turn on at least one of the upper light source, the lower light source and the ultraviolet light source according to the image acquisition instruction so as to acquire a colony image in the culture dish through the camera.

Optionally, the light source control method of the culture dish photographing device further includes:

receiving a fluorescence acquisition instruction sent by the terminal;

and controlling to turn on the upper light source and/or the ultraviolet light source according to the fluorescence acquisition instruction so as to acquire a fluorescence image of the bacterial colony in the culture dish through the camera.

Optionally, the controlling, according to the fluorescence collection instruction, to turn on the upper light source and/or the ultraviolet light source to collect a fluorescence image of a colony in the culture dish through the camera specifically includes:

and controlling to turn on the upper light source and/or the ultraviolet light source according to the fluorescence acquisition instruction, and carrying out continuous shooting or light source switching shooting so as to acquire the fluorescence image of the bacterial colony in the culture dish through the camera.

Optionally, the receiving the fluorescence acquisition instruction sent by the terminal specifically includes:

receiving a fluorescence acquisition instruction generated by a terminal according to the inherent color of the culture medium;

the control according to fluorescence acquisition instruction opens go up the light source and/or ultraviolet light source carries out continuous shooting or switches light source and shoots to gather through the camera the fluorescence image of bacterial colony in the culture dish specifically includes:

and controlling the upper light source and the ultraviolet light source to synthesize a light color most corresponding to the inherent color of the culture medium according to the fluorescence acquisition instruction.

According to the technical scheme, the culture dish cover is covered above the culture dish, and the camera of the camera is arranged in the convex part at the bottom of the culture dish cover, so that the small culture dish shooting device is formed and is convenient to carry; meanwhile, the light sources at different positions are arranged, so that different light sources can be conveniently controlled to work according to different scenes, and bacterial colony images meeting different requirements can be acquired.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Fig. 1 is a schematic structural diagram of a petri dish photographing device according to an embodiment of the invention.

Fig. 2 is a schematic structural diagram of a culture dish photographing device in an inverted state according to an embodiment of the invention.

Fig. 3 is a flowchart illustrating a light source control method of a culture dish photographing device according to an embodiment of the invention.

Detailed Description

The following further describes embodiments of the present invention with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and the protection scope of the present invention is not limited thereby.

Fig. 1 shows a schematic structural diagram of a culture dish photographing device provided in this embodiment, including: culture dish cover 1, camera 2, upper light source 3, lower light source 4 and ultraviolet light source 5.

The culture dish cover 1 is barrel-shaped, the upside-down cover is arranged above the culture dish 6, and the central part of the bottom surface 11 of the culture dish cover 1 is upwards protruded to form a convex part 111.

The camera of the camera 2 is arranged in the raised portion 111, which is arranged facing the culture dish 6.

The upper light source 3 is arranged above the culture dish cover 1 and is uniformly arranged around the camera, or is arranged outside the culture dish cover 1.

The lower light source 4 is arranged at the lower position of the culture dish.

The ultraviolet light source 5 is arranged on the outer side wall of the culture dish cover 1.

Specifically, the upper light source 3 and the lower light source 4 both adopt RGB composite light sources, and the brightness and color of the light sources can be adjusted through the RGB composite light sources, so that the characteristics of the light sources are changed, the influence of the ground color in the original environment is eliminated, and a clearer colony image is obtained.

By adopting the ultraviolet light source 5, the fluorescence phenomenon of specific microorganisms can be collected, and the colony images can be conveniently subjected to fluorescence analysis.

Specifically, through the reflected light of the upper light source 3, the colony image obtained by the camera is bright in the colony part and clear in the surface morphology of the colony; the transmitted light from the lower light source 4 causes the portion of the colony in the colony image captured by the camera to be dark, as opposed to the brightness of the colony image captured by the reflected light from the upper light source. By processing different light and shade in the reflected light image and the transmitted light image, a clearer colony image can be obtained.

Further, the irradiation of the ultraviolet light source 5 can generate a fluorescence phenomenon, and three different types of colony images can be obtained by combining the reflected light from the upper light source 3 and the transmitted light from the lower light source 4. When colony images are subsequently processed, the three colony images can be superposed to obtain multi-aspect colony information

When the upper light source 3 is provided on the bottom surface 11 inside the culture dish cover 1, a certain shadow is formed.

If the shadow does not affect the processing of the colony image, the upper light source 3 may be disposed on the bottom surface 11 inside the culture dish cover 1, otherwise, the upper light source 3 needs to be disposed on the inner sidewall of the culture dish cover 1 at a position close to the bottom surface 11, and the shadow does not exist after the colony image is captured.

The culture dish cover is disposable, is the tubbiness, and the back-off cover is established in the top of culture dish, forms relative closed environment with the culture dish, avoids the microbial contamination culture medium in the environment, and the culture medium is located the culture dish. The center of the barrel bottom is raised upwards; the camera of the camera is arranged in the convex part and faces the culture dish; the upper light source is arranged above the culture dish cover around the camera, or is arranged on the outer side of the culture dish cover, and a reflected light image on the surface of the culture medium can be obtained through irradiation of the upper light source; the lower light source is arranged below the culture dish, and a transmitted light image generated by light penetrating through the culture medium can be obtained through irradiation of the lower light source; the ultraviolet light source is arranged on the outer side wall of the culture dish cover, and an ultraviolet absorption image of the culture medium can be obtained when the ultraviolet light source irradiates.

In the embodiment, the culture dish cover is covered above the culture dish, and the camera of the camera is arranged in the convex part at the bottom of the culture dish cover, so that the small culture dish shooting device is formed and is convenient to carry; meanwhile, the light sources at different positions are arranged, so that different light sources can be conveniently controlled to work according to different scenes, and bacterial colony images meeting different requirements can be acquired.

Further, in the above embodiment, the head portion of the camera is protruded from a protruded portion provided at the center of the bottom surface of the culture dish cover.

Specifically, when the culture dish image capturing apparatus is placed upright as shown in fig. 1, water in the culture dish is easily evaporated, water vapor is formed inside the bottom surface 11 of the culture dish cover 1, and if the camera is placed on the same plane as the through hole 111, water vapor is easily formed on the camera, which greatly affects the image capturing effect. The head part of the camera is arranged in the culture dish cover in a protruding mode, namely the camera forms a protrusion in the culture dish cover 1, so that the camera is not beneficial to water vapor accumulation, and images with better shooting effects can be shot.

Further, on the basis of the above embodiment, the culture dish cover 1 has a hemispherical shape or a barrel shape.

In particular, the shape of the culture dish cover can be selected according to different requirements.

When the placing direction of the culture dish shooting device needs to be switched, a barrel-shaped culture dish cover as shown in fig. 1 and 2 can be adopted, and both sides can be placed.

When the culture dish replacing and shooting device is only arranged rightly, a hemispherical culture dish cover can be adopted, the through hole is arranged on a hemispherical spherical surface, and the culture dish cover in the shape can not be reversely arranged because the spherical surface can not be stably arranged on a horizontal plane.

Generally, a mask height of 10-12cm is preferred to facilitate the acquisition of more effective images.

Further, on the basis of the above embodiment, the culture dish photographing device further includes: communication means and information storage means.

When the terminal requests the colony image, the colony image may be transmitted to the terminal through the communication unit. Alternatively, the colony image is stored by the information storage unit.

Further, in the above embodiment, the camera of the camera is disposed above the culture dish for upright shooting, or the camera of the camera is disposed below the culture dish for inverted shooting.

Specifically, after the camera 2 is fixedly disposed in the culture dish cover, the culture dish may be placed in a right position, as shown in FIG. 1, or in a reverse position, as shown in FIG. 2.

When the culture dish is inverted, the culture cover 8 covers the underside of the culture dish as shown in FIG. 2.

Further, the culture dish shooting device further comprises a gyroscope;

Through the state of placing of gyroscope record culture dish shooting device, the state of placing of device is shot to convenient automatic acquisition culture dish.

Further, on the basis of the above embodiment, the culture medium of the culture dish is arranged facing the camera, and the camera acquires the microorganism image in the culture medium after being irradiated by the upper light source;

or the like, or, alternatively,

Specifically, the culture dish can be used for removing a culture dish cover and a culture cover to form a relatively closed system, placing the relatively closed system in equipment for shooting, wherein the surface of a culture medium faces a camera, and a growth image of microorganisms on the surface of the culture medium can be obtained through an upper light source; the culture medium can also be placed upside down below the culture hood, and the culture medium is positioned in the culture dish and faces to the bottom light source, and the transmission image of the growth of the microorganisms in the culture medium can be obtained through the irradiation of the bottom light source.

Further, on the basis of the above embodiment, a plurality of the culture dish photographing devices are arranged in the same incubator.

Specifically, the culture dish shooting device equipment that this embodiment provided is small in size, can arrange a plurality of culture dish shooting device in same incubator and cultivate to eliminate the influence of environmental factor difference to the microorganism growth phenomenon.

Further, on the basis of the above embodiment, the upper light source and the lower light source are both formed into a uniform surface light source by the light uniformizing plate.

Specifically, the upper light source and the lower light source form an even surface light source through the light homogenizing plate, and the influence of the formation of reflection light spots on the photographing effect is avoided.

Further, on the basis of the above embodiment, the culture dish photographing device further includes:

the temperature and humidity sensor, the light intensity sensor, the gas partial pressure sensor and the temperature and humidity alarm unit;

The temperature and humidity alarm unit is connected with the temperature and humidity sensor and alarms when the temperature and humidity are detected to deviate from a preset range.

Through setting up hygrothermograph and humiture alarm unit, make things convenient for real time monitoring culture dish to shoot the inside temperature of device, when temperature or humidity are unfavorable for the growth of bacterial colony, can in time report to the police.

Fig. 3 is a flowchart illustrating a light source control method of a culture dish photographing device according to the embodiment, and the method includes:

s301, receiving an image acquisition instruction sent by a terminal.

S302, controlling to turn on at least one of the upper light source, the lower light source and the ultraviolet light source according to the image acquisition instruction so as to acquire a colony image in the culture dish through the camera.

The image acquisition instruction is an instruction which is sent to the culture dish shooting device by the terminal and is used for acquiring a bacterial colony image.

Specifically, the ultraviolet light can collect the fluorescence phenomenon of specific microorganisms, so that the fluorescence analysis of a colony image is further facilitated.

This embodiment is through locating the top of culture dish cover in the culture dish to locate the camera of camera in the bulge of culture dish cover bottom, form miniature culture dish and shoot the device, through the light source that sets up different positions, conveniently according to the different light source work of different scene control, in order to obtain the bacterial colony image that satisfies different demands.

Further, on the basis of the above embodiment of the method, the method for controlling the light source of the culture dish photographing device further includes:

and receiving a fluorescence acquisition instruction sent by the terminal.

The fluorescence acquisition instruction is an instruction which is sent to the culture dish shooting device by the terminal and is used for acquiring a colony fluorescence image.

By controlling the work of different light sources, bacterial colony images meeting different requirements can be acquired.

Further, on the basis of the above method embodiment, the controlling, according to the fluorescence acquisition instruction, to turn on the upper light source and/or the ultraviolet light source to acquire a fluorescence image of a colony in the culture dish by the camera specifically includes:

Specifically, continuous shooting can be performed by a single or multiple light sources, for example, by turning on the upper light source and the ultraviolet light source, with both light sources; or switching the upper light source and the ultraviolet light source to carry out contact shooting.

Different shooting requirements can be met through different shooting modes.

In another embodiment, S301 specifically includes:

correspondingly, S302 specifically includes:

and controlling the upper light source and the ultraviolet light source to synthesize a light color corresponding to the inherent color of the culture medium according to the fluorescence acquisition instruction.

By controlling the upper light source and the ultraviolet light source to synthesize the light color corresponding to the inherent color of the culture medium, a better shooting effect can be obtained.

The above-described embodiments of the apparatus are merely illustrative, and the units described as separate parts may or may not be physically separate, and parts displayed as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of the present embodiment. One of ordinary skill in the art can understand and implement it without inventive effort.

Through the above description of the embodiments, those skilled in the art will clearly understand that each embodiment can be implemented by software plus a necessary general hardware platform, and certainly can also be implemented by hardware. With this understanding in mind, the above-described technical solutions may be embodied in the form of a software product, which can be stored in a computer-readable storage medium such as ROM/RAM, magnetic disk, optical disk, etc., and includes instructions for causing a computer device (which may be a personal computer, a server, or a network device, etc.) to execute the methods described in the embodiments or some parts of the embodiments.

It should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.

Claims

1. A culture dish photographing device, comprising: the culture dish comprises a culture dish cover, a camera, an upper light source, a lower light source and an ultraviolet light source;

the lower light source is arranged at the lower position of the culture dish;

2. The culture dish photographing device according to claim 1, wherein the head of the camera is protruded at a protruded portion of a center of a bottom surface of the culture dish cover.

3. The culture dish photographing device of claim 1, wherein the culture dish cover is hemispherical or barrel-shaped.

4. The culture dish imaging apparatus of claim 1, further comprising: a communication unit and an information storage unit;

5. The culture dish photographing device according to claim 1, wherein the camera is arranged above the culture dish for upright photographing or arranged below the culture dish for inverted photographing.

6. The culture dish imaging device of claim 5, further comprising a gyroscope;

7. The culture dish photographing device according to claim 1, wherein the culture medium of the culture dish is arranged to face the camera, and the camera acquires the microorganism image in the culture medium after being irradiated by the upper light source;

or the like, or, alternatively,

8. The culture dish imaging device of claim 1, wherein a plurality of the culture dish imaging devices are disposed in the same incubator.

9. The culture dish photographing device of claim 1, wherein the upper light source and the lower light source are both formed into a uniform surface light source by a light homogenizing plate.

10. The culture dish imaging apparatus of claim 1, further comprising: a temperature and humidity sensor, a light intensity sensor and a gas partial pressure sensor;

11. The culture dish imaging apparatus of claim 10, further comprising: a temperature and humidity alarm unit;

12. A light source control method of a culture dish photographing device according to any one of claims 1 to 11, comprising:

receiving an image acquisition instruction sent by a terminal;

13. The light source control method of the culture dish photographing device according to claim 12, further comprising:

receiving a fluorescence acquisition instruction sent by the terminal;

14. The light source control method of the culture dish photographing device according to claim 13, wherein the controlling of turning on the upper light source and/or the ultraviolet light source according to the fluorescence collection instruction to collect the fluorescence image of the colony in the culture dish by the camera includes:

15. The light source control method of the culture dish photographing device according to claim 13, wherein the receiving of the fluorescence acquisition command sent by the terminal specifically comprises:

correspondingly, the control according to fluorescence collection instruction opens go up the light source with ultraviolet light source carries out continuous shooting or switches the light source and shoots to gather through the camera the fluorescence image of the bacterial colony in the culture dish specifically includes: