CN110915733A - Method for artificial induced spawning, natural mating fertilization and embryo floating running water incubation - Google Patents
Method for artificial induced spawning, natural mating fertilization and embryo floating running water incubation Download PDFInfo
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- CN110915733A CN110915733A CN201910984006.XA CN201910984006A CN110915733A CN 110915733 A CN110915733 A CN 110915733A CN 201910984006 A CN201910984006 A CN 201910984006A CN 110915733 A CN110915733 A CN 110915733A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a method for fish artificial induced spawning, natural mating fertilization and embryo floating running water incubation, which comprises the following steps: 1) injecting oxytocin into parent fish with well developed gonad for artificial spawning induction; 2) putting the parent fish injected with oxytocin into a net cage with attachments, waiting for the parent fish to estrus and chase tail, and naturally spawning and fertilizing on the attachments; 3) transferring the attached fertilized eggs to a hatching main tank for embryo floating running water hatching. Compared with the traditional artificial insemination and incubation, the method provided by the invention has the advantages that the incubation rate of the fish fertilized eggs and the survival rate of the fish fertilized eggs after the fish fertilized eggs are put into a pond are obviously improved, the loss of parent fish is reduced, the labor cost is reduced, the method is suitable for continuous incubation and culture of viscous fish eggs, and the production efficiency of artificial culture of fish is favorably improved. The invention provides a new mode for fish genetic breeding work and has important significance in the aspect of fish genetic breeding.
Description
Technical Field
The invention belongs to the technical field of fish reproduction, and particularly relates to a method for artificial spawning induction, natural mating fertilization and embryo floating running water incubation.
Background
There are 32000 kinds of fishes in nature, and the kinds of fishes are the most varied among vertebrates. The fish is rich in protein, vitamins and essential minerals of human body, and simultaneously contains a large amount of essential amino acids, thereby being an important protein source for human beings. Most of the proteins are derived from artificial breeding, and the artificial breeding work of fishes is a crucial ring in the artificial breeding work.
At present, most of fish artificial breeding is traditional artificial induced spawning, artificial dry method/wet method insemination and hatching. The estrus time of the parent fish after induced spawning is in important relation with the maturity of the sexual gland of the fish, the dose of oxytocin, the water temperature and the like, and the estrus time is uncertain; the situation is often checked by pulling the net for many times, and parent fishes are easy to be injured. Artificial insemination needs to extrude parent fish from abdomen to cloaca, multiple times of extrusion easily causes problems of parent fish gonad damage, rib fracture, scale falling and the like, and the injured fish cannot be produced or even die in the next year; the eggs which are not completely mature are easily extruded by artificial insemination, the eggs have no fertilization capability, and water mold easily grows during hatching, so that the hatching of normal embryos is influenced, the hatching rate is low, the survival rate is not high, and the fry activity is low; artificial insemination requires many times of checking the parent fish to be spawned one by one, and ovum squeezing insemination consumes a lot of manpower. These problems have long hindered the development of fish farming and are to be addressed by those skilled in the art.
The viscous roe refers to roe with mucus or thread on the surface of egg membrane, such as roe produced by carp, crucian carp, bream, catfish, and culter. Due to the viscosity of the viscous fish eggs, fertilized eggs are always randomly adhered to the inner wall of the water pool, are easily eaten by big fish, are difficult to collect and transfer into a special hatching tank for high-efficiency hatching, and influence the production efficiency of artificial breeding.
Disclosure of Invention
The technical problem to be solved by the invention is to overcome the defects and shortcomings in the background technology, and provide the method for artificial induced spawning, natural mating fertilization and embryo floating running water incubation, which avoids the damage of parent fish caused by extrusion, has high incubation rate and high survival rate, and is suitable for the continuous incubation of adhesive fish eggs.
In order to solve the technical problems, the technical scheme provided by the invention is as follows:
an artificial induced spawning-natural mating fertilization-embryo floating running water hatching method comprises the following steps:
(1) putting female parent fish and male parent fish subjected to artificial induced spawning into the same induced spawning net cage in a round pond, arranging fertilized egg attachments in the induced spawning net cage, arranging running water with the water depth lower than the upper opening of the induced spawning net cage in the round pond (the running water can stimulate the parent fish to spawn and produce sperms), finishing natural mating and fertilization when the parent fish oestrous and finishes natural mating and fertilization (the parent fish can be prevented from being damaged by extrusion of artificial insemination and the survival rate is improved), naturally attaching the obtained fertilized eggs to the fertilized egg attachments of the induced spawning net cage (the egg membrane surface of the adhesive eggs has viscosity and can be adhered to the attachments), taking out the fertilized egg attachments when the fertilized eggs adhered to the fertilized egg attachments reach a sufficient quantity, then putting new fertilized egg attachments (continuously adhering the fertilized eggs) into the induced spawning net cage, and replacing the new female parent fish and the male parent fish subjected to artificial induced spawning in the induced spawning net cage after the batch of the female parent fish and the male parent fish stop spawning and the sperms (continuously mating and producing sperms) ) (ii) a
(2) Transferring the fertilized egg attachments attached with the fertilized eggs obtained in the step (1) into an incubation tank, completely immersing the fertilized egg attachments into water, performing embryo floating running water incubation, taking out the attachments which are not attached with the fertilized eggs after the embryos grow out, transferring the incubated fish fries to a breeding pond after the incubated fish fries grow out of the waist point, feeding the fish fries in the incubation tank, putting the fertilized egg attachments attached with the fertilized eggs into the incubation tank again, and continuing the embryo floating running water incubation.
Preferably, in the method, the female parent fish and the male parent fish are female parent fish and male parent fish of carp, crucian carp, bream, catfish and culter.
Preferably, in the step (1), the specific operation method of artificial induced spawning includes the following steps: selecting female parent fish and male parent fish with mature gonad development, injecting mixed oxytocic of 8-10ug/kg lutein release hormone analogue and 400 IU/kg chorionic gonadotropin from the root of pectoral fin to induce spawning, enabling a needle head to face to abdomen, injecting abdominal cavity, wrapping the head and gill part of the parent fish with a wet towel during injection, injecting the male parent fish firstly, and injecting the female parent fish after 3-5 h. The abdomen of the female parent fish with good gonad development is obviously convex (the fecundity is large), the abdomen is soft, and the milky semen flows out when the male parent fish with good gonad development lightly presses the abdomen. The artificial induced spawning method can shorten the estrus, mating and fertilization periods and effectively improve the spawning and sperm production efficiency of the parent fish.
Preferably, in the step (1), the fertilized egg attachments comprise palm sheets and/or mesh sheets, the aperture of the induced spawning net cage is 1-3cm, and the water temperature in the round pond is 23-26 ℃. The palm sheets and the net sheets are suitable for the adhesion of fertilized eggs; the induced spawning net cage with the aperture of 1-3cm can not only ensure the water flowing effect, but also effectively limit the swimming range of the parent fish; the water temperature of 23-26 ℃ is suitable for spawning, sperm production, mating and fertilization of parent fishes.
Preferably, in the step (1), a round tank overflow port is arranged at the center of a perfect circle of a round tank, a plurality of water inlets are respectively arranged near the outer wall of the round tank, the water inlet directions of the plurality of water inlets are all clockwise or anticlockwise directions of the round tank, an oxygen-enriched airflow pipeline is arranged at the bottom of the round tank, and an oxygen-enriched water flow pipeline is arranged at the periphery of the outer wall of the round tank; and a plurality of induced spawning net cages are placed in the round pond simultaneously. This round pool can form annular rivers, can carry out the induced spawning of a plurality of fish varieties simultaneously, or same fish divides the box to induced spawning, for the process of induced spawning provides effectual flowing water stimulation, promotes parent fish spawning and produces the essence to can provide rich oxygen, can induced spawning more fishes simultaneously and not the oxygen deficiency, improved the utilization ratio of round pool and the efficiency of induced spawning greatly, be fit for the industrialization and breed production.
Preferably, in the step (1), the fertilized egg attachments are suspended above the induced spawning net cage through ropes, so that the fertilized egg attachments are vertically suspended in the induced spawning net cage; in the step (2), the fertilized egg attachments are tied on long rods through ropes, and the long rods are transversely lapped above the incubation groove, so that the fertilized egg attachments are completely vertically suspended in the water of the incubation groove. Fertilized egg attachments are vertically suspended in the induced spawning net cage to cover each water layer, so that the fertilized egg attachments are favorable for effectively adhering fertilized eggs; fertilized egg attachments can not completely cover the periphery of the induced spawning net cage, and the effect of flowing water stimulation can be influenced.
Preferably, in the step (2), the temperature of the embryo in the floating running water hatching is 23-26 ℃. The temperature is suitable for hatching the fertilized eggs, and the hatching rate and the survival rate are improved.
In the above method, preferably, the hatching tank includes a main hatching tank, an overflow tank is disposed on a side surface of the main hatching tank, the main hatching tank and the overflow tank are separated by a gauze, an overflow port is disposed on a bottom or an outer wall of the overflow tank, a water discharge port through which the fry can pass is disposed on the bottom of the main hatching tank, and a water inlet pipe is disposed at a bottom of the main hatching tank. The density of the gauze is based on avoiding the fry from escaping; the water inlet pipe which is paved at the bottom of the whole hatching main tank flushes the fertilized egg attachments, so that the fertilized eggs are fully contacted with the dissolved oxygen in the water, and the hatching rate can be improved. More preferably, the length × width × height of the hatching main tank is 4 × 2 × 1.2m, and the vertical depth from the overflow port to the bottom of the hatching main tank is about 1 m.
More preferably, the top of the overflow groove is flush with the top of the hatching main groove, and the depth of the overflow groove is half of that of the hatching main groove; be equipped with a plurality of evenly distributed's inlet opening on the pipe wall of inlet tube, make whole hatching main tank evenly intake, with the flowing water direction control for by supreme down, do benefit to the hatchability and the fry survival rate that improve the embryonated egg.
More preferably, the gap passes through the pipeline and is connected with the end of intaking of No. 1 cistern, the water inlet pipe passes through the pipeline and is connected with the play water end of No. 2 cisterns, No. 1 cistern passes through the well pump with No. 2 cisterns and links to each other, the top of No. 1 cistern is less than the gap, the bottom of No. 2 cisterns is higher than the top of hatching the main trough. The water overflowing from the overflow port flows into the No. 1 reservoir, is pumped into the No. 2 reservoir through a water pump, and then flows into the hatching main tank through the water inlet pipe to realize circulating water flow; the higher the reservoir 2 is, the better, the water inlet pipe is favorable for water inlet, or the booster pump is arranged in the water inlet direction, so that the water inlet pressure is convenient to control, and the flowing speed of flowing water incubation is favorable to control.
More preferably, the overflow tank is a separate overflow tank, and the outer wall of each overflow tank unit of the separate overflow tank is provided with a plurality of overflow ports with different heights and controllable switches, so as to adjust the water level in the hatching main tank according to requirements. Compared with the integrated overflow tank, the separated overflow tank can reduce the water pressure bearing capacity of a single gauze, can effectively prolong the service life of the gauze, only needs targeted replacement when the gauze is damaged, does not need to replace the gauze of the whole overflow tank, and saves the cost.
Compared with the prior art, the invention has the beneficial effects that:
1. the method provided by the invention has the advantages that natural mating fertilization is adopted, damage to parent fish caused by extrusion of artificial fertilization is avoided, the operation is simple, the production cost is low, the method is suitable for continuous hatching and breeding of viscous fish eggs, the hatching rate is high, the survival rate is high, and the improvement of the production efficiency of artificial breeding of fish is facilitated.
2. The circular pool adopted by the invention can form annular water flow, can simultaneously induce the spawning of a plurality of fish varieties or induce the spawning of the same fish in a net cage, provides effective running water stimulation for the induced spawning process, promotes the spawning and the sperm production of parent fish, can provide rich oxygen, and is suitable for industrial culture production.
3. The movable fertilized egg attachments are arranged in the induced spawning net cage, so that the induced spawning net cage is convenient to hang and transfer, and can effectively adhere and collect the fertilized eggs.
4. The hatching tank adopted by the invention has a simple structure, can be used for fish embryo floating running water hatching, is convenient for replacing fertilized egg attachments and collecting hatched fish fries, has large hatching amount and high hatching efficiency, effectively avoids the loss of the fertilized eggs and the fish fries, can realize circulating running water, improves the contact between the fertilized eggs and oxygen in water, saves water resources, is beneficial to reducing the production cost, and is suitable for continuous fish hatching and breeding.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic diagram of a round pool structure used in an embodiment of the present invention;
FIG. 2 is a schematic diagram of a square induced spawning net cage used in the embodiment of the invention;
FIG. 3 is a schematic diagram of a circular induced spawning cage used in an embodiment of the present invention;
FIG. 4 is a schematic diagram of a planar structure of a hatching tank used in an embodiment of the present invention;
FIG. 5 is a schematic perspective view of a hatching tank used in an embodiment of the present invention.
Illustration of the drawings:
1. a square induced spawning net cage; 2. a circular induced spawning net cage; 3. a round tank overflow port; 4. the outer wall of the round pool; 5. an oxygen-enriched airflow pipeline; 6. an oxygen-enriched airflow outlet; 7. an oxygen-enriched water flow pipeline; 8. an aeration water outlet; 9. a water inlet; 10. hatching the main tank; 11. an overflow tank; 12. a screen; 13. an overflow port; 14. a water discharge outlet; 15. a water inlet pipe; 16. fertilized egg attachments; 17. a long stick;
1-1, a square net cage top cover; 1-2, a square net cage base; 1-3, square net cage feet; 2-1, a circular net cage top cover; 2-2, a round net cage base; 2-3, round net cage feet.
Detailed Description
In order to facilitate understanding of the invention, the invention will be described more fully and in detail with reference to the accompanying drawings and preferred embodiments, but the scope of the invention is not limited to the specific embodiments below.
Unless otherwise defined, all terms of art used hereinafter have the same meaning as commonly understood by one of ordinary skill in the art. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of the present invention.
Unless otherwise specifically stated, various raw materials, reagents, instruments, equipment and the like used in the present invention are commercially available or can be prepared by existing methods.
Example (b):
the invention relates to an artificial induced spawning-natural mating fertilization-embryo floating running water hatching method, which comprises the following steps:
(1) artificial hastening parturition: in the breeding season of 2018, pulling a net to fish, selecting female parent fish with mature gonads (the abdomen is soft and expanded and the male parent fish can slightly extrude milky semen), carrying out induced spawning on the female parent fish, injecting a mixed induced spawning agent of lutein release hormone analogue (9ug/kg) and chorionic gonadotropin (500IU/kg) from the root of a pectoral fin to carry out induced spawning, enabling a needle head to face the abdomen to carry out intraperitoneal injection, wrapping the head and the gill part of the parent fish by using a wet towel during injection, injecting the male parent fish firstly, and then injecting the female parent fish after 3 hours;
(2) natural mating and fertilization: putting the female parent fish and the male parent fish after artificial spawning induction into the same spawning induction net cage in the round pond, wherein fertilized egg attachments 16 such as palm sheets or net sheets are arranged in the spawning induction net cage, and the fertilized egg attachments 16 are hung above the spawning induction net cage through ropes, so that the fertilized egg attachments 16 are vertically suspended in the spawning induction net cage; the round pond is internally provided with running water (the running water temperature is 23-26 ℃) the depth of which is lower than the upper opening of the induced spawning net box, the running water stimulates the parent fish to play a role of oxytocin; after the parent fishes are oestrous and tailed and complete natural mating and fertilization, naturally attaching the obtained fertilized eggs to fertilized egg attachments 16 of an induced spawning net cage, checking the spawning condition on the attachments after 8-12h, taking out the fertilized egg attachments 16 after the fertilized eggs attached to the fertilized egg attachments 16 reach a sufficient number, then putting new fertilized egg attachments 16 into the induced spawning net cage, and replacing new female parent fishes and male parent fishes subjected to artificial induced spawning in the induced spawning net cage after the mating of the female parent fishes and the male parent fishes in the batch is completed;
(3) embryo floating running water incubation: tying the fertilized egg attachments 16 attached with the fertilized eggs obtained in the step (2) on long rods 17 through ropes, enabling the long rods 17 to be transversely lapped above an incubation groove, enabling the fertilized egg attachments 16 to be completely vertically suspended in water in the incubation groove, feeding water from the bottom of the incubation groove to ensure that the fertilized egg attachments 16 are in a suspended state, overflowing the water from the upper part, and carrying out flowing water incubation (the flowing water incubation control temperature is 23-26 ℃) until fertilized egg embryos are incubated; the overflow port is sealed by dense gauze to prevent the fry from escaping; after the embryos are all hatched out of the fry, taking out the attachments, and simultaneously turning down a water inlet valve; after the hatched fry grow out of the waist point, the fry are discharged from a water outlet at the bottom of the hatching main tank and transferred to a pond for further culture; fertilized egg attachments 16 to which fertilized eggs are attached are newly placed in the hatching tank, and the floating running water hatching of the embryo is continued.
In the round pond (as shown in fig. 1) adopted in this embodiment, the diameter of the round pond is 10m, the water depth of the round pond is 1m, the center of a perfect circle is provided with an overflow port 3, a plurality of water inlets 9 are respectively arranged near the outer wall 4 of the round pond, the water inlet directions of the plurality of water inlets 9 are both clockwise or counterclockwise of the round pond, the bottom of the round pond is provided with an oxygen-enriched airflow pipeline 5, oxygen-enriched airflow outlets 6 are uniformly distributed on the oxygen-enriched airflow pipeline 5, oxygen-enriched water flow pipelines 7 are arranged on the periphery of the outer wall 4 of the round pond, and oxygen-enriched water flow outlets 8 are uniformly distributed on the oxygen; a plurality of square induced spawning net cages 1 (shown in figure 2) or round induced spawning net cages 2 (shown in figure 3) are simultaneously placed in the round pool. The square induced spawning net cage 1 is 3.2m multiplied by 1.4m multiplied by 1.3m in length, width and height and comprises a box body, a square net cage top cover 1-1, a square net cage base 1-2 and square net cage feet 1-3; the diameter of the circular induced spawning net cage 2 is 0.9-1.4 m, the height of the circular induced spawning net cage is 1.3m, and the circular induced spawning net cage comprises a box body, a circular net cage top cover 2-1, a circular net cage base 3-2 and circular net cage bottom feet 3-3.
The round pond can form annular running water, and can simultaneously induce the inbreeding of different types of fishes and distant hybridization among species; the round pond is provided with air flow oxygenation and water flow oxygenation pipelines, so that oxygen supply of parent fishes in the net cage is fully ensured; the net cages with the same size can induce spawning of more fishes without oxygen deficiency, so that the utilization rate of the round pond and the spawning inducing efficiency are greatly improved, and the method is suitable for industrial aquaculture production.
The hatching tank (as shown in fig. 4-5) adopted in this embodiment includes a main hatching tank 10, the main hatching tank 10 has a length × width × height of 4m × 2m × 1.2m, and a water depth of 1m, an overflow tank 11 (a plurality of independent separate overflow tanks) is provided on a side surface of the main hatching tank 10, the main hatching tank 10 is separated from the overflow tank 11 by a gauze 12 to prevent the fry from escaping and losing, an overflow port 13 is provided on a bottom or an outer wall of each overflow tank unit of the separate overflow tanks 11 (a plurality of overflow ports 13 capable of being controlled to be switched at different heights may be provided on an outer wall of the overflow tank 11 to adjust a water level in the main hatching tank according to needs), a top of the overflow tank 11 is flush with a top of the main hatching tank 10, and a depth of the overflow tank 11 is half of a depth of the main hatching tank 10; the bottom of the hatching main tank 10 is provided with a water outlet 14 for fish fry to pass through, and is also provided with a water inlet pipe 15 which is paved at the bottom of the whole hatching main tank 10, and the pipe wall of the water inlet pipe 15 is provided with a plurality of water inlet holes which are uniformly distributed. The overflow port 13 is connected with the water inlet end of the No. 1 water storage tank through a pipeline, the water inlet pipe 15 is connected with the water outlet end of the No. 2 water storage tank through a pipeline, and the bottom of the No. 2 water storage tank is higher than the top of the hatching main groove 10. The fertilized egg attachments 16 are tied on long rods 17 through ropes, and the long rods 17 are transversely lapped above the incubation groove, so that the fertilized egg attachments 16 are completely vertically suspended in the water of the incubation groove.
The main hatching tank 10 can be integrated, so that the same fish species can be conveniently cultured in a large scale; or a plurality of independent hatching chambers can be formed by the net, each hatching chamber is provided with an independent overflow trough 11, an overflow port 13, a water inlet pipe 15 and a water outlet 14, and a plurality of hatching chambers can hatch different fish species at the same time, thereby being convenient for hatching and breeding by species.
The hatching tank is convenient for replacing fertilized egg attachments and collecting hatched fries, has large hatching amount and high hatching efficiency, can realize circulating water, improves the contact between the fertilized eggs and oxygen in water, saves water resources and is beneficial to reducing production cost.
To further prove the effect of the present invention, when natural mating and fertilization are performed, the parent fish are fished out for artificial insemination, and the obtained fertilized eggs are incubated in regular running water as a control, and the corresponding data such as fertilization rate, hatching rate and the like are counted, as shown in table 1. 1000 seedlings are placed in the same environment, after 3 months of cultivation, the survival rate is checked by pulling a net, and the results are shown in table 1. The statistical result shows that compared with the artificial insemination method, the average fertility rate, the hatchability and the survival rate of the fry in the later culture period are greatly improved.
Table 1: fish fry fertility rate, hatchability and survival rate under different modes
The method successfully avoids the uncertainty of the estrus time of the fish, does not need net pulling inspection and artificial insemination, and saves the labor cost; the process of egg-extruding insemination is omitted, the damage of the parent fish is reduced, and the loss of the parent fish is reduced; compared with artificial insemination, natural spawning and hatching improve the hatchability of fish fertilized eggs and the survival rate after the fish fertilized eggs are put into a pond, hatched fry is more robust, and the survival rate in the later period is improved; meanwhile, the method also improves the utilization rate of the induced spawning circular pool and the induced spawning efficiency, provides a new mode for fish genetic breeding work, has important application value in the aspect of fish genetic breeding, and is beneficial to improving the production efficiency of artificial fish culture.
Claims (10)
1. An artificial induced spawning-natural mating fertilization-embryo floating running water hatching method is characterized by comprising the following steps:
(1) putting the female parent fish and the male parent fish subjected to artificial induced spawning into the same induced spawning net cage in a round pond, wherein fertilized egg attachments (16) are arranged in the induced spawning net cage, running water with the water depth lower than the opening on the induced spawning net cage is arranged in the round pond, after the parent fish has oestrous and tailgating and finishes natural mating and fertilization, the obtained fertilized eggs are naturally attached to the fertilized egg attachments (16) of the induced spawning net cage, after the fertilized eggs attached to the fertilized egg attachments (16) reach a sufficient amount, taking out the fertilized egg attachments (16), then putting new fertilized egg attachments (16) into the induced spawning net cage, and after the spawning and spermatogenesis of the batch of female parent fish and male parent fish stop, replacing new female parent fish and male parent fish subjected to artificial induced spawning in the induced spawning net cage;
(2) transferring the fertilized egg attachments (16) attached with the fertilized eggs obtained in the step (1) into an incubation tank, completely immersing the fertilized egg attachments (16) into water, incubating the embryos in floating running water, taking out the attachments which are not attached with the fertilized eggs after the embryos grow out, transferring the fish fries to a breeding pond after the hatched fish fries grow out of the waist point, breeding the fish fries in the incubation tank, putting the fertilized egg attachments (16) attached with the fertilized eggs into the incubation tank again, and continuing the incubation in floating running water.
2. The method according to claim 1, wherein in the step (1), the specific operation method of artificial induced spawning comprises the following steps: selecting female parent fish and male parent fish with mature gonad development, injecting mixed oxytocic of 8-10ug/kg lutein release hormone analogue and 400 IU/kg chorionic gonadotropin from the root of pectoral fin to induce spawning, enabling a needle head to face to abdomen, injecting abdominal cavity, wrapping the head and gill part of the parent fish with a wet towel during injection, injecting the male parent fish firstly, and injecting the female parent fish after 3-5 h.
3. The method according to claim 1, wherein in the step (1), the fertilized egg attachments (16) comprise palm sheets and/or mesh sheets, the diameter of the spawning cage is 1-3cm, and the water temperature in the round pond is 23-26 ℃.
4. The method according to claim 1, wherein in the step (1), a round tank overflow port (3) is arranged at the center of a perfect circle of a round tank, a plurality of water inlets (9) are respectively arranged near the outer wall (4) of the round tank, the water inlet directions of the plurality of water inlets (9) are all clockwise or anticlockwise directions of the round tank, an oxygen-enriched airflow pipeline (5) is arranged at the bottom of the round tank, and an oxygen-enriched water flow pipeline (7) is arranged at the periphery of the outer wall (4) of the round tank; and a plurality of induced spawning net cages are placed in the round pond simultaneously.
5. The method according to claim 1, wherein in the step (1), the fertilized egg attachment (16) is suspended above the induced spawning cage by a rope, so that the fertilized egg attachment (16) is vertically suspended in the induced spawning cage; in the step (2), the fertilized egg attachments (16) are tied on long rods (17) through ropes, and the long rods (17) are transversely lapped above the incubation tank, so that the fertilized egg attachments (16) are completely vertically suspended in the water of the incubation tank.
6. The method according to claim 1, wherein in the step (2), the incubation temperature of the embryos in floating running water is 23-26 ℃.
7. The method according to any one of claims 1 to 6, wherein the hatching tank comprises a main hatching tank (10), an overflow tank (11) is arranged on the side of the main hatching tank (10), the main hatching tank (10) is separated from the overflow tank (11) by a gauze (12), an overflow port (13) is arranged on the bottom or outer wall of the overflow tank (11), a water discharge port (14) for passing the fry is arranged on the bottom of the main hatching tank (10), and a water inlet pipe (15) is arranged on the bottom of the whole main hatching tank (10).
8. Method according to claim 7, characterized in that the top of said overflow tank (11) is flush with the top of the hatching main tank (10), said overflow tank (11) having a depth half that of the hatching main tank (10); the pipe wall of the water inlet pipe (15) is provided with a plurality of water inlet holes which are uniformly distributed.
9. The method according to claim 7, characterized in that the overflow port (13) is connected with the water inlet end of the No. 1 water reservoir through a pipeline, the water inlet pipe (15) is connected with the water outlet end of the No. 2 water reservoir through a pipeline, the No. 1 water reservoir is connected with the No. 2 water reservoir through a well pump, the top of the No. 1 water reservoir is lower than the overflow port (13), and the bottom of the No. 2 water reservoir is higher than the top of the hatching main tank (10).
10. A method according to claim 7, characterized in that the gutter (11) is a separate gutter with a number of on-off controllable weirs (13) of different heights on the outer wall of each gutter unit.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN114097697A (en) * | 2021-08-27 | 2022-03-01 | 华南农业大学 | Non-contact fish breeding system |
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| CN111657185A (en) * | 2020-05-28 | 2020-09-15 | 湖南师范大学 | Method for cultivating hybrid bream |
| CN112640834A (en) * | 2020-12-25 | 2021-04-13 | 嘉鱼县罗家桥养殖专业合作社 | Device and method for increasing yield of leiocassis longirostris fry |
| CN112913734A (en) * | 2021-02-02 | 2021-06-08 | 山东省海洋生物研究院 | Natural egg mass hatching method for viscous eggs |
| CN114097697A (en) * | 2021-08-27 | 2022-03-01 | 华南农业大学 | Non-contact fish breeding system |
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