CN110903963A - Novel domestic multiple pathogenic bacteria typing detect reagent box - Google Patents

Novel domestic multiple pathogenic bacteria typing detect reagent box Download PDF

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Publication number
CN110903963A
CN110903963A CN201911379312.7A CN201911379312A CN110903963A CN 110903963 A CN110903963 A CN 110903963A CN 201911379312 A CN201911379312 A CN 201911379312A CN 110903963 A CN110903963 A CN 110903963A
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swab
culture medium
cutting plate
blade
detection kit
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CN201911379312.7A
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Chinese (zh)
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熊尚岷
朱云
李仕铭
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Suzhou Wei Shan Biotechnology Co Ltd
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Suzhou Wei Shan Biotechnology Co Ltd
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Priority to CN201911379312.7A priority Critical patent/CN110903963A/en
Publication of CN110903963A publication Critical patent/CN110903963A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/10Enterobacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/14Streptococcus; Staphylococcus
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/24Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • G01N2333/25Shigella (G)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/24Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • G01N2333/255Salmonella (G)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/305Assays involving biological materials from specific organisms or of a specific nature from bacteria from Micrococcaceae (F)
    • G01N2333/31Assays involving biological materials from specific organisms or of a specific nature from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/315Assays involving biological materials from specific organisms or of a specific nature from bacteria from Streptococcus (G), e.g. Enterococci

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  • Proteomics, Peptides & Aminoacids (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Toxicology (AREA)
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Abstract

The invention provides a novel household multiple pathogenic bacteria typing detection kit which comprises a sealed box body, wherein the box body comprises a typing indication panel, a swab cutting plate, a liquid culture medium chamber and a plurality of solid culture medium subchambers; the solid culture medium chamber is mainly used for selective culture of pathogenic bacteria; whether the pathogenic bacteria are infected or not can be judged according to the indication of the typing indication panel. The kit is practical at home, convenient and quick to sample, can quickly identify various common pathogenic bacteria in life, can be used as a reference for guiding medication, and avoids indiscriminate use and abuse of antibiotic medicines.

Description

Novel domestic multiple pathogenic bacteria typing detect reagent box
Technical Field
The invention relates to a disposable kit for typing detection of multiple domestic pathogenic bacteria, and belongs to the field of biomedical devices.
Background
The food-borne pathogenic bacteria are visible everywhere in life and are one of the main microbial indexes for controlling the safety of food and medicines. For pathogenic reactions caused by ingestion of food or drugs containing food-borne pathogenic bacteria and common pathogenic bacteria in life, the phenomenon that a patient cannot take corresponding antibacterial drugs to effectively treat due to possible indiscriminate use and abuse of antibiotic drugs and incapability of taking corresponding antibacterial drugs in time is caused because the pathogenic bacteria are not known. Therefore, a detection kit capable of rapidly identifying common pathogenic bacteria in life needs to be designed so that a patient can take corresponding antibacterial drugs. At present, no multi-pathogen typing detection kit which can be used at home is available on the market.
Disclosure of Invention
The invention aims to provide a novel household multiple pathogenic bacteria typing detection kit to solve the technical problem that in the prior art, a patient cannot timely take antibiotic medicines aiming at pathogenic bacteria to cause that diseases cannot be effectively treated due to the fact that the pathogenic reaction is not known to be caused by which pathogenic bacteria.
In order to achieve the purpose, the invention adopts the following technical scheme:
a novel household multiple pathogenic bacteria typing detection kit is characterized by comprising a sealed box body, wherein a transverse separation baffle and a plurality of longitudinal separation baffles are arranged in the box body, the transverse separation baffle separates the interior of the box body into a liquid culture medium chamber and a solid culture medium chamber, the longitudinal separation baffles separate the solid culture medium chamber into a plurality of solid culture medium subchambers, baffle openings are arranged at the middle upper part of the transverse separation baffle, and the baffle openings are arranged to enable the liquid culture medium chamber to be communicated with each solid culture medium subchamber; the front side wall of each solid medium subchamber is provided with a typing indication panel for detecting pathogenic bacteria; the upper side wall of the liquid culture medium chamber is provided with an opening, the opening is covered with a reciprocating swab cutting plate, a swab inlet corresponding to the opening is formed in the swab cutting plate, and the swab inlet can be communicated or not communicated with the opening by moving the swab cutting plate; the swab inlet is provided with a blade, and the cutting direction of the blade is the same as the moving direction of the swab cutting plate; the swab cutting plate is also provided with a poking plate used for moving the swab cutting plate.
Further, still be provided with on the swab cutting board with the bar mouth of swab entry intercommunication, the width of bar mouth is less than the width of swab entry, the blade comprises two first blade portions and second blade portion, and two first blade portions symmetry set up the both sides of bar mouth, second blade portion be located the bar mouth with one side that the swab entry is relative, two first blade portions all are connected with second blade portion.
Further, the distance between the front end edges of the two first blade parts close to the swab inlet is smaller than the distance between the rear end edges thereof.
Further, the knife edge of the second blade part is V-shaped.
Further, the blade and paddle are disposed on opposite sides of the swab inlet.
Further, the swab cutting plate is in sliding connection with the upper side wall of the liquid medium chamber.
Further, the upper side wall of the liquid culture medium chamber is provided with two parallel concave slide rails, and the swab cutting plate is provided with two convex slide bars which are respectively in sliding fit with the two concave slide rails.
Furthermore, a plurality of baffle openings are arranged, the number of the baffle openings is the same as that of the solid culture medium subchambers, and the baffle openings and the solid culture medium subchambers are arranged in a one-to-one correspondence manner; alternatively, the baffle opening is provided in one piece, and the length of the baffle opening is set to be enough to cover all the solid medium subchambers.
Further, the pathogenic bacteria comprise any one or a combination of salmonella, staphylococcus aureus, hemolytic streptococcus and shigella.
Further, the liquid medium chamber is provided with a liquid medium for enrichment culture of pathogenic bacteria, and the liquid medium comprises the following components: each 1L of distilled water contains 10-30 g of peptone, 20-30 g of tryptone, 5-115 g of sodium chloride, 0.5-3.0 g of glucose, 1.5-5.5 g of monopotassium phosphate, 5-10 g of sodium chloride, 3.0-6.0 g of beef extract, 800.8-3.5 mL of tween, 1.0-10.0 g of disodium hydrogen phosphate dodecahydrate and 1000mL of distilled water.
Further, each solid medium subchamber is provided with a solid medium for selective cultivation of pathogenic bacteria, and the solid medium comprises but is not limited to any one of the following groups: (1) 0.2-0.8% of L-lysine, 0.01-0.10% of ferric ammonium citrate, 0.068-0.07% of sodium thiosulfate and 0.001-0.0016% of phenol red; (2) neutral red 0.001% -0.009%, crystal violet 0.0001% -0.00015%; (3) 1-20% of rabbit blood and soybean meal agar; (4) 0.1% -0.6% of mixed pigment.
Further, each typing indicator panel comprises a control zone and a test zone, the control zone may be displayed as any one of, but not limited to, the following strips: (1) pink or yellow; (2) colorless to light pink; (3) golden yellow (sometimes white); (4) a combination of red and green-blue-green.
Further, the kit is a cuboid, the length of the kit is 5-15 cm, the width of the kit is 3-6 cm, and the height of the kit is 2-6 cm.
The novel household multiple pathogenic bacteria typing detection kit is high in practicability and convenient and rapid to sample, can be used for rapidly identifying multiple common pathogenic bacteria in life, can be used as a reference for guiding medication, and avoids indiscriminate use and abuse of antibiotic medicines.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a novel domestic multi-pathogen typing detection kit according to an embodiment of the present invention;
FIG. 2 is a schematic view of the swab cutting plate of FIG. 1;
FIG. 3 is a schematic view of the swab configuration;
FIG. 4 is a schematic view of the typing indicator panel for detecting multiple pathogens of FIG. 1;
FIG. 5 is a schematic view showing test results of the typing indication panels in FIG. 1;
FIG. 6 is a flow chart showing the use of the novel home-use multiple pathogenic typing detection kit shown in FIG. 1;
FIG. 7 is a schematic view of the inactivation process of the novel household multi-pathogenic typing detection kit shown in FIG. 1.
Wherein, 1 type indicator panel; 2, a swab cutting plate; 3 a liquid medium chamber; 4 solid medium subchamber; 5 transverse separating baffles; 6, opening a baffle; 7 a swab inlet; 8 a first blade portion; 9 a second blade portion; 10, a poking plate; 11, a swab; 12 strip-shaped openings; 13 sterilizing and exhausting holes; 14 a cover; 15 blades; 16 longitudinal dividing baffles; and 17, packaging the box body.
Detailed Description
The invention is further described with reference to specific examples. The following examples are only for illustrating the technical solutions of the present invention more clearly, and the protection scope of the present invention is not limited thereby.
As shown in figure 1, a novel domestic multiple pathogenic bacteria typing detect reagent box, including a sealed box body, the box body is the cuboid (including but not only being limited to this shape), and length 5~15cm, wide 3~6cm are high for 2~6cm, and the total volume is less, and is small and exquisite at home, conveniently carries.
The box body is inside to be provided with horizontal separation baffle 5 and a plurality of vertical partition baffle 16, horizontal separation baffle 5 is liquid medium room 3 and solid medium room with box body internal separation, vertical partition baffle 16 separates the solid medium room for a plurality of solid medium subchambers 4, liquid medium room 3 is used for increasing the fungus of pathogenic bacterium to cultivate, solid medium subchamber 4 is used for the selective culture of pathogenic bacterium, liquid medium room 3 and solid medium subchambers 4 are mutually independent, the fungus and the mutual noninterference of selective culture of the fungus of increasing of pathogenic bacterium have been guaranteed.
Baffle opening 6 is preset in the middle upper part of transverse separation baffle 5, and the length of baffle opening 6 is set to make liquid medium chamber 3 communicate with each solid medium subchamber 4.
In another embodiment, the baffle openings may be provided in a plurality, the number being the same as the number of solid media subchambers 4, and the plurality of baffle openings are provided in one-to-one correspondence with the plurality of solid media subchambers 4.
The upper side wall of the liquid medium chamber 3 is provided with an opening on which a movable swab cutting plate 2 is arranged. Preferably, the swab cutting plate 2 is slidably connected to the upper side wall of the liquid medium chamber 3. The sliding connection mechanism can adopt the prior art, for example, two parallel concave sliding rails are arranged on the upper side wall of the liquid culture medium chamber 3, two convex sliding bars which are respectively in sliding fit with the two concave sliding rails are arranged on the swab cutting board, and the reciprocating movement of the swab cutting board 2 is realized through the sliding fit of the concave sliding rails and the convex sliding bars.
As shown in fig. 2, the swab cutting board 2 is provided with a swab inlet 7, the swab inlet 7 corresponds to an opening on the upper side wall of the liquid medium chamber 3, the swab inlet 7 can be communicated or not communicated with the opening by moving the swab cutting board 2 back and forth, when the swab inlet 7 is not communicated with the opening, the opening is shielded by the swab cutting board 2, the opening is closed, and the box body is in a sealed state; when the swab inlet 7 is in communication with the aperture, the aperture is opened and a swab 11 (shown in fig. 3) may be inserted into the aperture from the swab inlet 7.
The swab cutting plate 2 is further provided with a blade 15 having a blade edge facing the swab inlet 7 for cutting the swab 11 inserted at the swab inlet 7. The swab 11 is generally made of, but not limited to, sterile absorbent cotton and a wooden stick, is easy to cut, is easy to operate, does not need to go to a hospital, and can be used at home to sample saliva, oral mucosa and the like. The swab cutting plate 2 is also provided with a poking plate 10 for moving the swab cutting plate 2, and the swab cutting plate 2 can be moved back and forth by poking the poking plate 10. Preferably, the blade 15 and the toggle plate 10 are disposed on either side of the swab inlet 7.
In a preferred embodiment, the swab cutting plate 2 is further provided with a strip-shaped opening 12 communicating with said swab inlet 7, the strip-shaped opening 12 having a width smaller than the width of the swab inlet 7. The blade 15 is formed by two first blade portions 8 and a second blade portion 9, the two first blade portions 8 being symmetrically arranged on both sides of the strip-shaped opening 12, the second blade portion 9 being located on the opposite side of the strip-shaped opening 12 from the swab entrance 7, both first blade portions 8 being connected to the second blade portion 9. The blade is arranged in such a way that the swab 11 is enclosed between the two first blade parts 8 and the second blade part 9, which further facilitates the severing of the swab 11 when the swab cutting plate 2 is pushed in movement by the striking plate 10.
In a preferred embodiment, the distance between the front end edges of the two first blade portions 8 close to the swab inlet 7 is smaller than the distance between the rear end edges thereof. In this way, the swab 11 can be cut first by the knife edge front end, making it easier to cut the swab 11 when the third blade part 9 is slid into contact with the swab 11.
In a preferred embodiment, the knife edge of the second blade part 9 is V-shaped, and this structure can, on one hand, clamp the swab 11 in the middle of the knife edge to avoid the movement of the swab during cutting, and on the other hand, can reduce the contact area between the knife edge and the swab 11 and increase the pressure, so that the swab 11 can be cut off more easily by the second blade part 9.
The front side wall of each solid medium subchamber 4 is provided with a typing indication panel 1. As shown in fig. 4, the typing indication panel 1 includes a test area (test) and a control area (control), and according to the control strip indication, it can be quickly distinguished whether the sample contains pathogenic bacteria (including but not limited to any combination of common pathogenic bacteria such as salmonella, staphylococcus aureus, streptococcus hemolyticus, shigella, etc.) detected by the kit. By providing a plurality of solid medium subchambers 4 and a plurality of typing indication panels 1, multiple pathogenic bacteria can be simultaneously detected. There are four cases of the detection result of the typing indication panel 1: positive, negative, weak positive and invalid, as shown in fig. 6, the one with the signal of both Control and Test is positive, and can be used as a medication reference, and if no indication appears, the detection fails, and the re-detection is recommended. Wherein, the indication area control may be displayed as any one of the following bands (but not limited to the following bands): (1) pink or yellow; (2) colorless to light pink; (3) golden yellow (sometimes white); (4) a combination of red and green-blue-green.
The liquid medium chamber 3 is a main area for culturing pathogenic bacteria, and aims to enable pathogenic bacteria flora to reach detectable concentration. The liquid culture medium chamber 3 is internally provided with a liquid culture medium, and the liquid culture medium mainly comprises but is not limited to: each 1L of distilled water contains 10-30 g of peptone, 20-30 g of tryptone, 5-115 g of sodium chloride, 0.5-3.0 g of glucose, 1.5-5.5 g of monopotassium phosphate, 5-10 g of sodium chloride, 3.0-6.0 g of beef extract, 0.8-3.5 mL of Tween 80 (Tween 80) and 1.0-10.0 g of disodium hydrogen phosphate (containing 12 crystal water), and culturing for 18-48 hours.
The solid medium sub-chamber 4 is a selective culture chamber, and different dyes can be added according to different requirements to display different colors (including but not limited to colors) so as to achieve the effect of prejudgment. The solid culture medium subchamber 4 mainly comprises agar (including but not limited to agar coagulants) culture medium, and is composed of basic culture medium and specific reagents, including but not limited to any one of the following groups: (1) 0.2-0.8% of L-lysine, 0.01-0.10% of ferric ammonium citrate, 0.068-0.07% of sodium thiosulfate and 0.001-0.0016% of phenol red; (2) neutral red 0.001% -0.009%, crystal violet 0.0001% -0.00015%; (3) 1-20% of rabbit blood and soybean meal agar; (4) 0.1-0.6% of mixed pigment, and culturing for 18-72 h.
The use method of the novel household multiple pathogenic bacteria typing detection kit comprises the following steps:
as shown in fig. 6, saliva, oral mucosa and the like are sampled by using a sterile swab 11, after sampling, the swab cutting plate 2 is slid by pulling the poking plate 10 to enable the swab inlet 7 to be communicated with an opening on the upper side wall of the liquid culture medium chamber 3, the swab 11 is inserted into the swab inlet 7, the swab cutting plate 2 is slid to enable the blade 15 to move towards the swab 11 to cut off the swab 11, the swab 11 falls into the liquid culture medium chamber 3, the kit is slightly shaken to enable the swab 11 and the liquid culture medium to be fully mixed, after enrichment culture is carried out for a certain time (left figure), the kit is turned over to a parting indication panel 1 downwards (right figure), the liquid culture medium is enabled to flow into each solid culture medium subchamber 4 through a reserved baffle opening 6 to carry out selective culture, the parting indication panel 1 of each solid culture medium subchamber 4 reacts with a pathogenic bacterium which is selectively cultured, and a detection result is displayed, the detection result is checked through the typing indication panel 1, and whether the pathogenic bacteria are infected or not can be judged according to the indication of the typing indication panel 1.
The invention also provides an external packaging box matched with the household multiple pathogenic bacteria typing detection kit, and the external packaging box not only can be used as a package of the kit, but also can be used as a container for sterilizing the used kit.
As shown in fig. 7, the outer package box includes an outer package box body 17 and a lid 14 provided on the outer package box body 17. The lid 14 is detachably or turnably attached to the outer package box. The lid 14 is provided with a sterile vent 13.
The kit of the present invention is further provided with a self-heating pack containing self-heating materials, the main components including but not limited to: CaO, Na2CO3Iron powder, aluminum powder, coke powder, activated carbon, salt and the like. The temperature of a common self-heating bag sold in the market can be maintained at 100-200 ℃, the heating time is 3-5 minutes, 4-5 self-heating bags can be provided for the kit, so that the continuous and stable boiling heating time is maintained for 20-40 minutes, and the effect of full sterilization is achieved.
After the kit is used, it is sterilized using an external pack and a self-heating pack. The lid 14 is opened, water is added to the outer package case 17, the self-heating pack is put into the outer package case 17, and then the reagent cartridge is put into the outer package case 17, and the lid 14 is closed. The water is added to automatically boil the tea, so that the tea has a sterilization effect, and is convenient and quick. The lid 14 needs to be covered during the high temperature heating process, which is cautious to prevent scalding. The sterilization vent hole 13 can prevent explosion caused by excessive pressure in the outer packing box due to heating. And pouring out the liquid in the box after the outer packing box is cooled, and discarding the box.
The present invention has been disclosed in terms of the preferred embodiment, but is not intended to be limited to the embodiment, and all technical solutions obtained by substituting or converting equivalents thereof fall within the scope of the present invention.

Claims (9)

1. A novel household multiple pathogenic bacteria typing detection kit is characterized by comprising a sealed box body, wherein a transverse separation baffle and a plurality of longitudinal separation baffles are arranged in the box body, the transverse separation baffle separates the interior of the box body into a liquid culture medium chamber and a solid culture medium chamber, the longitudinal separation baffles separate the solid culture medium chamber into a plurality of solid culture medium subchambers, baffle openings are arranged at the middle upper part of the transverse separation baffle, and the baffle openings are arranged to enable the liquid culture medium chamber to be communicated with each solid culture medium subchamber; the front side wall of each solid medium subchamber is provided with a typing indication panel for detecting pathogenic bacteria; the upper side wall of the liquid culture medium chamber is provided with an opening, the opening is covered with a reciprocating swab cutting plate, a swab inlet corresponding to the opening is formed in the swab cutting plate, and the swab inlet can be communicated or not communicated with the opening by moving the swab cutting plate; the swab inlet is provided with a blade, and the cutting direction of the blade is the same as the moving direction of the swab cutting plate; the swab cutting plate is also provided with a poking plate used for moving the swab cutting plate.
2. The domestic multi-pathogen typing detection kit according to claim 1, wherein a strip-shaped opening communicating with the swab inlet is further provided on the swab cutting plate, the width of the strip-shaped opening is smaller than the width of the swab inlet, the blade is composed of two first blade portions and a second blade portion, the two first blade portions are symmetrically provided on two sides of the strip-shaped opening, the second blade portion is provided on the opposite side of the strip-shaped opening from the swab inlet, and both first blade portions are connected with the second blade portion.
3. The novel domestic multiple pathogen typing detection kit according to claim 2, wherein the distance between the front end edges of the two first blade portions near the swab inlet is smaller than the distance between the rear end edges thereof.
4. The novel domestic multiple pathogen typing detection kit according to claim 3, wherein the edge of the second blade portion is V-shaped.
5. The novel domestic multiple pathogen typing detection kit according to claim 1, wherein said blade and said poke plate are separately provided at opposite sides of said swab inlet.
6. The novel home multiple pathogen typing detection kit according to claim 1, wherein the swab cutting plate is slidingly connected with the upper sidewall of the liquid medium chamber.
7. The novel domestic multiple pathogen typing detection kit according to claim 6, wherein the upper side wall of the liquid medium chamber is provided with two parallel concave slide rails, and the swab cutting plate is provided with two convex slide bars which are respectively in sliding fit with the two concave slide rails.
8. The novel household multiple pathogen typing detection kit according to claim 1, wherein the baffle openings are provided in a plurality, the number of the baffle openings is the same as the number of the solid culture medium subchambers, and the baffle openings and the solid culture medium subchambers are arranged in one-to-one correspondence; alternatively, the baffle opening is provided in one piece, and the length of the baffle opening is set to be enough to cover all the solid medium subchambers.
9. A novel domestic multi-pathogen typing detection kit according to any one of claims 1 to 8, wherein the kit is a cuboid having a length of 5 to 15cm, a width of 3 to 6cm and a height of 2 to 6 cm.
CN201911379312.7A 2019-12-27 2019-12-27 Novel domestic multiple pathogenic bacteria typing detect reagent box Pending CN110903963A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111358501A (en) * 2020-04-14 2020-07-03 青岛大学附属医院 Novel collector for nasopharynx swabs of infectious disease patients and using method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111358501A (en) * 2020-04-14 2020-07-03 青岛大学附属医院 Novel collector for nasopharynx swabs of infectious disease patients and using method thereof

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