CN110898458A

CN110898458A - Two aqueous phase extraction centrifuging tube

Info

Publication number: CN110898458A
Application number: CN201911312721.5A
Authority: CN
Inventors: 谢琳娜; 张迅捷; 谢茹胜
Original assignee: Fujian Vocational College Of Bioengineering
Current assignee: Fujian Vocational College Of Bioengineering
Priority date: 2019-12-18
Filing date: 2019-12-18
Publication date: 2020-03-24

Abstract

The invention relates to a double aqueous phase extraction centrifuge tube, which comprises a test tube and a sampling tube; the tube body of the test tube is an ellipsoid; the sampling tube comprises a sampling cylinder, a sampling bottom plate and a bending rod; the sampling bottom plate comprises a permeation plate and a liquid sealing plate; the side edge of the permeation sheet is in contact with the inner wall of the sampling tube and can slide on the inner wall of the sampling tube along the axial direction of the sampling tube; the liquid sealing sheet is arranged at the bottom of the permeation sheet; the bending rod can be tightly attached to the outer wall of the test tube after being bent; still include the bottle lid, be provided with the screw thread on the top outer wall of test tube, the bottle lid pass through the screw thread with the test tube connects soon. The invention can conveniently take away the upper liquid and simultaneously can avoid taking away the lower liquid.

Description

Two aqueous phase extraction centrifuging tube

Technical Field

The invention relates to a double aqueous phase extraction centrifugal tube, and belongs to the field of extraction tools.

Background

An Aqueous two-phase extraction (ATPS) technique is also called an Aqueous two-phase separation technique, and is an attractive and promising novel separation technique in recent years. The ATPE technology starts in the 60's of the 20 th century, and the double-water phase extraction and separation technology is applied to biological product separation from the double-water phase system discovered by Albertsson university of sweden in 1956 to Kula of GBF in Germany in 1979. So far, the aqueous two-phase system has been widely applied in the aspects of organic matter separation and purification such as biochemistry, cell biology, biochemical engineering and the like. The traditional high polymer/high polymer or high polymer/inorganic salt aqueous two-phase system has been successfully used in the fields of separating and extracting various proteins (polypeptide and enzyme), separating and extracting various antibiotics, amino acids, metals and effective components of traditional Chinese medicines, etc.

When two aqueous phase extraction, need use the centrifuging tube, but among the prior art, reagent is by the centrifugation branch liquid back, generally takes away upper liquid through the straw, but the test tube gets liquid the case comparatively inconvenient, and gets lower liquid easily.

Disclosure of Invention

In order to solve the technical problems, the invention provides a double-aqueous-phase extraction centrifugal tube which can conveniently take away supernatant liquid and can avoid taking away subnatant liquid.

The technical scheme of the invention is as follows: a double aqueous phase extraction centrifuge tube comprises a test tube and a sampling tube; the tube body of the test tube is an ellipsoid;

the sampling tube comprises a sampling cylinder, a sampling bottom plate and a bending rod; the sampling tube is inserted into the test tube and is tightly attached to the inner wall of the test tube; a limiting groove is formed in the inner wall of the top part in the test tube, and a bent part capable of being hung on the limiting groove is formed in the top end of the sampling tube;

the sampling bottom plate comprises a permeation plate and a liquid sealing plate; the side edge of the permeation sheet is in contact with the inner wall of the sampling tube and can slide on the inner wall of the sampling tube along the axial direction of the sampling tube;

the liquid sealing sheet is arranged at the bottom of the permeation sheet; one end of the bending rod is vertically and fixedly arranged at the midpoint of the liquid sealing sheet, and the bending rod vertically penetrates through the midpoint of the permeation sheet;

a plurality of groups of through holes arranged in a fan shape are formed in the penetrating sheet, and the size and shape of the liquid sealing sheet are the same as the arrangement shape of the through holes; when the liquid sealing sheet is rotated through the bending rod, the liquid sealing sheet can block or open the through hole;

the bending rod can be tightly attached to the outer wall of the test tube after being bent, a limiting ring is further sleeved on the bending rod, and the limiting ring is in contact with the permeation piece, so that the permeation piece can be pushed to move downwards when the bending rod is pushed downwards;

still include the bottle lid, be provided with the screw thread on the top outer wall of test tube, the bottle lid pass through the screw thread with the test tube connects soon.

Furthermore, the bending rods are three-section type bending rods, and the multiple sections of bending rods are mutually pivoted.

Further, be provided with an accepting groove on the outer wall of test tube for accept the pole of buckling.

Further, be provided with the screw thread on the pole of buckling, when the pole of buckling set up in the accepting groove, the screw thread on the pole of buckling with screw thread on the test tube is continuous mutually.

Furthermore, the outer ring of the permeation piece is wrapped by a silica gel ring.

The invention has the beneficial effects that: the liquid can be taken at the first time after the working procedure is finished, the influence of the substances exposed to the air for too long time is avoided, and meanwhile, the liquid can be taken directly, so that the operation is convenient; through the setting of sample film, the liquid depth that needs to take away is fixed a position that can be accurate, realizes comparatively accurate liquid of getting.

Drawings

FIG. 1 is a cross-sectional view of the present invention;

FIG. 2 is a cross-sectional view of a sampling tube of the present invention

FIG. 3 is an enlarged view taken at A of FIG. 2 in accordance with the present invention;

FIG. 4 is a block diagram of a permeable sheet of the present invention;

FIG. 5 is a structural view of a sealing sheet according to the present invention;

the reference numbers in the figures denote:

1. a test tube; 11. an accommodating groove;

2. a sampling tube; 21. a sampling tube; 22. sampling a negative film; 221. a permeable sheet; 2211. a through hole; 222. sealing the liquid sheet; 23. A bending rod; 231. a limiting ring;

3. and (7) a bottle cap.

Detailed Description

The invention is described in detail below with reference to the figures and the specific embodiments.

Referring to fig. 1-5, an aqueous two-phase extraction centrifuge tube comprises a test tube 1 and a sampling tube 2; the tube body of the test tube 1 is an ellipsoid;

the sampling tube 2 comprises a sampling cylinder 21, a sampling bottom sheet 22 and a bending rod 23; the sampling tube 21 is inserted into the test tube 1 and is tightly attached to the inner wall of the test tube 1; a limiting groove is formed in the inner wall of the top of the test tube 1, and a bent part capable of being hung on the limiting groove is formed in the top end of the sampling tube 21;

the sampling bottom plate 22 comprises a permeation sheet 221 and a liquid sealing sheet 222; the side edge of the permeation sheet 221 contacts with the inner wall of the sampling tube 21 and can slide on the inner wall of the sampling tube 21 along the axial direction of the sampling tube 21;

the liquid sealing sheet 222 is disposed at the bottom of the permeable sheet 221; one end of the bending rod 23 is vertically and fixedly arranged at the midpoint of the liquid sealing sheet 222, and the bending rod 23 vertically passes through the midpoint of the permeation sheet 221;

a plurality of groups of through holes 2211 arranged in a fan shape are formed in the permeation sheet 221, and the size and shape of the liquid sealing sheet 222 are the same as the arrangement shape of the through holes 2211; when the liquid sealing sheet 222 is rotated by the bending rod 23, the liquid sealing sheet 222 can block or open the through hole 2211;

after the bending rod 23 is bent, the bending rod can be tightly attached to the outer wall of the test tube 1, a limiting ring 231 is further sleeved on the bending rod 23, and the limiting ring 231 is in contact with the permeation sheet 221, so that the permeation sheet 221 can be pushed to move downwards when the bending rod 23 is pushed downwards;

still include bottle lid 3, be provided with the screw thread on the top outer wall of test tube 1, bottle lid 3 through the screw thread with 1 meet soon of test tube.

The working process of the invention is as follows: firstly, adding a reagent to be extracted, then inserting a sampling tube 2, placing the sampling bottom sheet 22 on the top of a sampling tube 21, bending the bent tube to be tightly attached to the outer wall of the test tube, screwing a bottle cap 3, carrying out centrifugal extraction, straightening the bent tube according to the position of the top liquid level after the centrifugal extraction is finished, pushing the sampling bottom sheet 22 through the bent rod 23, and controlling the liquid sealing sheet 222 to rotate by rotating the bent rod 23 in the pushing process so that the through holes 2211 on the permeation sheet 221 are all in an open state; when the sampling base plate 22 is at the position where liquid separation is needed, the bending rod 23 is rotated to control the liquid sealing sheet 222 to rotate so that the through holes 2211 on the permeation sheet 221 are all in a closed state, and at this time, the sampling tube 2 is taken out, and the process is completed.

The invention has the beneficial effects that: the liquid can be taken at the first time after the working procedure is finished, the influence of the substances exposed to the air for too long time is avoided, and meanwhile, the liquid can be taken directly, so that the operation is convenient; through the arrangement of the sampling bottom plate 22, the depth of the liquid to be taken away can be accurately positioned, and more accurate liquid taking is realized.

Furthermore, the bending rod 23 is a three-section bending rod 23, and the multiple sections of bending rods 23 are pivotally connected to each other.

As can be seen from the above description, through the setting of the three-section type bending rod 23, the bending rod 23 can be better arranged outside the test tube 1, and the interference of the bending rod 23 to the centrifugal process is avoided.

Further, the outer wall of the test tube 1 is provided with a receiving groove 11 for receiving the bending rod 23.

As can be seen from the above description, the accommodating groove 11 is configured to enable the bending rod 23 to be better installed outside the test tube 1, thereby further avoiding the influence of the bending rod 23 on the centrifugation process.

Further, a thread is disposed on the bending rod 23, and when the bending rod 23 is disposed in the accommodating groove 11, the thread on the bending rod 23 is continuous with the thread on the test tube 1.

As can be seen from the above description, by providing the thread on the bending rod 23, the bending rod 23 can be fixed when the bottle cap 3 is screwed, and the screwing effect of the bottle cap 3 is better.

Further, the outer ring of the permeation sheet 221 is wrapped with a silica gel ring.

As can be seen from the above description, the arrangement of the silicone ring can make the tight adhesion between the permeation sheet 221 and the sampling tube 21 better.

Example one

An aqueous two-phase extraction centrifuge tube as shown in fig. 1-5 comprises a test tube 1 and a sampling tube 2; the tube body of the test tube 1 is an ellipsoid; the sampling tube 2 comprises a sampling cylinder 21, a sampling bottom sheet 22 and a bending rod 23; the sampling tube 21 is inserted into the test tube 1 and is tightly attached to the inner wall of the test tube 1; a limiting groove is formed in the inner wall of the top of the test tube 1, and a bent part capable of being hung on the limiting groove is formed in the top end of the sampling tube 21; the sampling bottom plate 22 comprises a permeation sheet 221 and a liquid sealing sheet 222; the side edge of the permeation sheet 221 contacts with the inner wall of the sampling tube 21 and can slide on the inner wall of the sampling tube 21 along the axial direction of the sampling tube 21; the liquid sealing sheet 222 is disposed at the bottom of the permeable sheet 221; one end of the bending rod 23 is vertically and fixedly arranged at the midpoint of the liquid sealing sheet 222, and the bending rod 23 vertically passes through the midpoint of the permeation sheet 221; a plurality of groups of through holes 2211 arranged in a fan shape are formed in the permeation sheet 221, and the size and shape of the liquid sealing sheet 222 are the same as the arrangement shape of the through holes 2211; when the liquid sealing sheet 222 is rotated by the bending rod 23, the liquid sealing sheet 222 can block or open the through hole 2211; after the bending rod 23 is bent, the bending rod can be tightly attached to the outer wall of the test tube 1, a limiting ring 231 is further sleeved on the bending rod 23, and the limiting ring 231 is in contact with the permeation sheet 221, so that the permeation sheet 221 can be pushed to move downwards when the bending rod 23 is pushed downwards; the test tube is characterized by further comprising a bottle cap 3, wherein threads are arranged on the outer wall of the top of the test tube 1, and the bottle cap 3 is screwed with the test tube 1 through the threads; the bending rods 23 are three-section type bending rods 23, and the multiple sections of bending rods 23 are mutually pivoted; an accommodating groove 11 is formed in the outer wall of the test tube 1 and used for accommodating the bending rod 23; the bending rod 23 is provided with a thread, and when the bending rod 23 is arranged in the accommodating groove 11, the thread on the bending rod 23 is continuous with the thread on the test tube 1; the bending rod 23 is provided with the threads, so that the bending rod 23 can be fixed when the bottle cap 3 is screwed, and the screwing effect of the bottle cap 3 is better; through the setting of silica gel circle, can make infiltration piece 221 with the tight effect of subsides between the sampling tube 21 is better.

The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes performed by the present specification and drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims

1. The utility model provides a two aqueous phase extraction centrifuging tube which characterized in that: comprises a test tube (1) and a sampling tube (2); the tube body of the test tube (1) is an ellipsoid;

the sampling tube (2) comprises a sampling cylinder (21), a sampling bottom sheet (22) and a bending rod (23); the sampling tube (21) is inserted into the test tube (1) and is tightly attached to the inner wall of the test tube (1); a limiting groove is formed in the inner wall of the top of the test tube (1), and a bent part capable of being hung on the limiting groove is arranged at the top end of the sampling tube (21);

the sampling bottom plate (22) comprises a permeable sheet (221) and a liquid sealing sheet (222); the side edge of the permeation sheet (221) is in contact with the inner wall of the sampling cylinder (21) and can slide on the inner wall of the sampling cylinder (21) along the axial direction of the sampling cylinder (21);

the liquid sealing sheet (222) is arranged at the bottom of the permeation sheet (221); one end of the bending rod (23) is vertically and fixedly arranged at the midpoint of the liquid sealing sheet (222), and the bending rod (23) vertically penetrates through the midpoint of the permeation sheet (221);

a plurality of groups of through holes (2211) arranged in a fan shape are formed in the permeation sheet (221), and the size and shape of the liquid sealing sheet (222) are the same as the arrangement shape of the through holes (2211); when the liquid sealing sheet (222) is rotated by the bending rod (23), the liquid sealing sheet (222) can block or open the through hole (2211);

the bending rod (23) can be tightly attached to the outer wall of the test tube (1) after being bent, a limiting ring (231) is further sleeved on the bending rod (23), and the limiting ring (231) is in contact with the permeation sheet (221), so that the permeation sheet (221) can be pushed to move downwards when the bending rod (23) is pushed downwards;

still include bottle lid (3), be provided with the screw thread on the top outer wall of test tube (1), bottle lid (3) through the screw thread with test tube (1) connect soon.

2. The aqueous two-phase extraction centrifuge tube of claim 1, wherein: the bending rods (23) are three-section type bending rods (23), and the multi-section type bending rods (23) are mutually pivoted.

3. The aqueous two-phase extraction centrifuge tube of claim 1, wherein: the outer wall of the test tube (1) is provided with an accommodating groove (11) for accommodating the bending rod (23).

4. The aqueous two-phase extraction centrifuge tube of claim 3, wherein: the bending rod (23) is provided with threads, and when the bending rod (23) is arranged in the accommodating groove (11), the threads on the bending rod (23) are continuous with the threads on the test tube (1).

5. The aqueous two-phase extraction centrifuge tube of claim 1, wherein: the outer ring of the permeation piece (221) is wrapped by a silica gel ring.