CN110896840A - Method for improving polyphenol content in germinated barley by adjusting wavelength of irradiation light source - Google Patents
Method for improving polyphenol content in germinated barley by adjusting wavelength of irradiation light source Download PDFInfo
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- CN110896840A CN110896840A CN201911244506.6A CN201911244506A CN110896840A CN 110896840 A CN110896840 A CN 110896840A CN 201911244506 A CN201911244506 A CN 201911244506A CN 110896840 A CN110896840 A CN 110896840A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
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Abstract
The invention discloses a method for improving polyphenol content in malted barley by adjusting the wavelength of an irradiation light source, which comprises the steps of soaking barley to enable the barley to absorb water; and germinating under the irradiation of composite light of blue light with the intensity of 6000lx and the wavelength of 430-480 nm and red light with the wavelength of 620-720 nm. The method is simple, rapid and efficient, the germination rate is improved by 25 percent after 72 hours of germination compared with the traditional method, the germination time of the barley is shortened, the germination cost is reduced, the polyphenol with biological activity in the germinated barley is rapidly accumulated, the content of the polyphenol is improved, and the quality of the germinated barley is improved.
Description
Technical Field
The invention belongs to the technical field of barley germination, and particularly relates to a method for improving polyphenol content in germinated barley by adjusting the wavelength of an irradiation light source.
Background
Barley (Hordeum vulgare), belonging to the genus Hordeum (Hordeum) family of Poaceae and the genus Triticum family of Gramineae, is one of ancient crops cultivated by human beings, and has high comprehensive utilization rate. Barley is also one of grains with a large variety and content of phenolic substances, and the barley product is the best functional food for preventing and treating human chronic diseases. The phenolic compounds in barley mainly include benzoic acid and styrenate derivatives, procyanidin, quinones, flavonols, chalcones, flavones, flavanones, aminophenol compounds, etc. The polyphenol is a ubiquitous secondary metabolite in plants, and has a certain unique physiological effect in the aspects of oxidation resistance, cancer resistance, aging resistance, blood sugar reduction, blood fat reduction, cardiovascular disease prevention and the like.
The germination is a processing mode for improving the texture and the nutritional value of grains, the germination obviously influences the polyphenol content and the biological activity of barley, and the germinated barley can be used for brewing beer, medicine and digestion promotion. However, the conventional germination method has a long period and cannot achieve a good effect. According to the invention, the barley is treated by optimizing irradiation with different wavelengths to germinate, so that the polyphenol with biological activity in the germinated barley is rapidly accumulated, and the nutritional quality of the germinated barley is improved.
Disclosure of Invention
This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. In this section, as well as in the abstract and the title of the invention of this application, simplifications or omissions may be made to avoid obscuring the purpose of the section, the abstract and the title, and such simplifications or omissions are not intended to limit the scope of the invention.
In one aspect of the invention, the invention provides a method for increasing the polyphenol content of malted barley by adjusting the wavelength of the irradiation light source.
In order to solve the technical problems, the invention provides the following technical scheme: a method for increasing the polyphenol content of malted barley by adjusting the wavelength of an irradiation light source, which comprises,
soaking barley to make barley absorb water;
and germinating under the irradiation of composite light of blue light with the intensity of 6000lx and the wavelength of 430-480 nm and red light with the wavelength of 620-720 nm.
As a preferred embodiment of the method for increasing the polyphenol content in malted barley according to the present invention by adjusting the wavelength of the irradiation light source: the illumination intensity of the blue light and the red light is 1: 1.
as a preferred embodiment of the method for increasing the polyphenol content in malted barley according to the present invention by adjusting the wavelength of the irradiation light source: also comprises selecting full and intact barley, and soaking in sodium hypochlorite solution for sterilization before soaking barley to make barley absorb water.
As a preferred embodiment of the method for increasing the polyphenol content in malted barley according to the present invention by adjusting the wavelength of the irradiation light source: the mass concentration of the sodium hypochlorite solution is 0.05%.
As a preferred embodiment of the method for increasing the polyphenol content in malted barley according to the present invention by adjusting the wavelength of the irradiation light source: and (4) sterilizing for 20-40 min.
As a preferred embodiment of the method for increasing the polyphenol content in malted barley according to the present invention by adjusting the wavelength of the irradiation light source: also comprises washing the sterilized barley with water.
As a preferred embodiment of the method for increasing the polyphenol content in malted barley according to the present invention by adjusting the wavelength of the irradiation light source: the barley is soaked to make the barley absorb water, and the soaking temperature is room temperature.
As a preferred embodiment of the method for increasing the polyphenol content in malted barley according to the present invention by adjusting the wavelength of the irradiation light source: and the germination is realized by flatly paving the barley on a container filled with gauze for germination, wherein the germination time is 72-96 hours.
As a preferred embodiment of the method for increasing the polyphenol content in malted barley according to the present invention by adjusting the wavelength of the irradiation light source: and also comprises sprinkling water every 24h in the germination process, and treating the box body with ozone.
As a preferred embodiment of the method for increasing the polyphenol content in malted barley according to the present invention by adjusting the wavelength of the irradiation light source: and during germination, the germination temperature is controlled to be 25 ℃, and the relative humidity is controlled to be 90%.
The invention has the beneficial effects that: the method is simple, rapid and efficient, the germination rate is improved by 25 percent after 72 hours of germination compared with the traditional method, the germination time of the barley is shortened, the germination cost is reduced, the polyphenol with biological activity in the germinated barley is rapidly accumulated, the content of the polyphenol is improved, and the quality of the germinated barley is improved.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with examples are described in detail below.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.
Furthermore, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one implementation of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.
Example 1:
selecting 100g of full and complete barley, and soaking the barley in a 0.05% sodium hypochlorite solution for disinfection treatment for 30 minutes:
washing the barley treated by the sodium hypochlorite solution with deionized water to remove the sodium hypochlorite solution attached to the surface of the barley;
soaking the washed barley for 24 hours in deionized water at 25 ℃ to ensure that the barley absorbs water fully;
the barley is spread on a container with gauze and germinated in the dark in an environment with a temperature of 25 ℃ and a humidity of 90%, for 96 hours, sprayed with water every 24 hours, washed with gauze, and treated with ozone for 20 minutes to prevent mildew. After germination for 24h, 48h, 72h and 96h, the polyphenol content is 0.71, 0.88, 1.06 and 1.06(mgGAE/g), and the oxidation resistance is 0.19, 0.2, 0.21 and 0.24 (mu mol/g).
The method for testing the polyphenol content comprises the following steps: crushing germinated barley, adding an organic reagent to extract polyphenol, taking 0.2mL of barley phenol extracting solution, adding 3mL of deionized water and 0.2mL of 1.0M Folin-Ciocalteu reagent, uniformly mixing, standing for 8min, adding 0.6mL of 7.5% (M/V) sodium carbonate solution, reacting in a dark place for 1h, and measuring a light absorption value at 760 nm.
The oxidation resistance test method comprises the following steps: DHPP is taken as a free radical to measure the antioxidant activity of polyphenol, a water-soluble vitamin E (Trolox) solution is taken as a standard substance, 20mg of DPPH reagent is accurately weighed, absolute ethyl alcohol is used for fixing the volume to a 500mL volumetric flask, and the solution is shaken up and stored in a brown flask for later use. Mixing 0.2mL of sample solution to be detected and 3.8mL of DPPH solution, shaking uniformly, reacting at room temperature for 1h, and measuring absorbance A at wavelength of 517nms,Absorbance A of DPPH solution and an equal volume (0.2mL) of distilled water was measured simultaneously0And the absorbance Ar of the solvent (absolute ethanol) of 0.2mL of the solution to be tested and 3.8mL of the DPPH solution, expressed as the amount of Trolox substance (μmol) per gram of barley.
Example 2:
selecting 100g of full and complete barley, and soaking the barley in a 0.05% sodium hypochlorite solution for disinfection treatment for 30 minutes:
washing the barley treated by the sodium hypochlorite solution with deionized water to remove the sodium hypochlorite solution attached to the surface of the barley;
soaking the washed barley for 24 hours in deionized water at 25 ℃ to ensure that the barley absorbs water fully;
the water-absorbed barley was spread on a container with gauze, germinated for 96 hours under irradiation of red light (700nm) with an illumination intensity of 6000lx in an environment of 25 ℃ and 90% humidity, sprayed with water every 24 hours, and the gauze was washed and treated with ozone for 20 minutes to prevent mold. After germination for 24h, 48h, 72h and 96h, the polyphenol content is respectively 0.74, 0.9, 1.1 and 1.07(mgGAE/g), and the oxidation resistance is respectively 0.21, 0.2, 0.23 and 0.24 (mu mol/g).
Example 3:
selecting 100g of full and complete barley, and soaking the barley in a 0.05% sodium hypochlorite solution for disinfection treatment for 30 minutes:
washing the quinoa treated by the sodium hypochlorite solution with deionized water, and washing away the sodium hypochlorite solution attached to the surface of the barley;
soaking the washed barley for 24 hours in deionized water at 25 ℃ to ensure that the barley absorbs water fully;
the water-absorbed barley was spread on a container with gauze, germinated for 96 hours under irradiation of yellow light (580nm) with a light intensity of 6000lx in an environment of 25 ℃ and a humidity of 90%, sprayed with water every 24 hours, and the gauze was washed and treated with ozone for 20 minutes to prevent mold. After germination for 24h, 48h, 72h and 96h, the polyphenol content is respectively 0.68, 0.8, 0.97 and 1.03(mgGAE/g), and the oxidation resistance is respectively 0.18, 0.19, 0.2 and 0.22 (mu mol/g).
Example 4:
selecting 100g of full and complete barley, and soaking the barley in a 0.05% sodium hypochlorite solution for disinfection treatment for 30 minutes:
washing the quinoa treated by the sodium hypochlorite solution with deionized water, and washing away the sodium hypochlorite solution attached to the surface of the barley;
soaking the washed barley for 24 hours in deionized water at 25 ℃ to ensure that the barley absorbs water fully;
the water-absorbed barley was spread on a container with gauze, germinated for 96 hours under the irradiation of green light (530nm) with an illumination intensity of 6000lx in an environment of 25 ℃ and 90% humidity, sprayed with water once every 24 hours, and the gauze was washed and treated with ozone for 20 minutes to prevent mold. After germination for 24h, 48h, 72h and 96h, the polyphenol content is respectively 0.7, 0.89, 0.99 and 1.01(mgGAE/g), and the oxidation resistance is respectively 0.2, 0.19, 0.21 and 0.23 (mu mol/g).
Example 5:
selecting 100g of full and complete barley, and soaking the barley in a 0.05% sodium hypochlorite solution for disinfection treatment for 30 minutes:
washing the barley treated by the sodium hypochlorite solution with deionized water to remove the sodium hypochlorite solution attached to the surface of the barley;
soaking the washed barley for 24 hours in deionized water at 25 ℃ to ensure that the barley absorbs water fully; the water-absorbed barley was spread on a container with gauze, germinated for 96 hours under irradiation of blue light (460nm) with a light intensity of 6000lx in an environment of 25 ℃ and 90% humidity, sprayed with water every 24 hours, and the gauze was washed and treated with ozone for 20 minutes to prevent mold. After germination for 24h, 48h, 72h and 96h, the polyphenol content is respectively 0.72, 0.87, 1.03 and 0.99(mgGAE/g), and the oxidation resistance is respectively 0.17, 0.19, 0.24 and 0.23 (mu mol/g).
Example 6:
selecting 100g of full and complete barley, and soaking the barley in a 0.05% sodium hypochlorite solution for disinfection treatment for 30 minutes:
washing the barley treated by the sodium hypochlorite solution with deionized water to remove the sodium hypochlorite solution attached to the surface of the barley;
soaking the washed barley for 24 hours in deionized water at 25 ℃ to ensure that the barley absorbs water fully;
the water-absorbed barley was spread on a gauze-padded container in an environment of 25 ℃ and 90% humidity under blue light (460nm) with an illumination intensity of 6000 lx: red (700nm) illumination intensity of 1: 1, sprouting for 96 hours, spraying water every 24 hours, cleaning gauze, and treating with ozone for 20 minutes to prevent mildew. After germination for 24h, 48h, 72h and 96h, the polyphenol content is respectively 0.89, 1.14, 1.37 and 1.35(mgGAE/g), and the oxidation resistance is respectively 0.29, 0.31, 0.37 and 0.37 (mu mol/g). The polyphenol content obtained by the method is increased to a great extent compared with other methods. The combination of the red light and the blue light can promote the elongation growth of plants, is beneficial to the accumulation of soluble sugar and simultaneously is beneficial to the extension of leaves and the inhibition of the elongation of stems, leads to the change of phytochrome, and then promotes the germination of seeds.
It should be noted that the above-mentioned embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the claims of the present invention.
Claims (10)
1. A method for increasing the polyphenol content in malted barley by adjusting the wavelength of an irradiation light source is characterized in that: comprises the steps of (a) preparing a mixture of a plurality of raw materials,
soaking barley to make barley absorb water;
and germinating under the irradiation of composite light of blue light with the intensity of 6000lx and the wavelength of 430-480 nm and red light with the wavelength of 620-720 nm.
2. The method of increasing the polyphenol content of malted barley as claimed in claim 1 wherein the irradiation light source wavelength is adjusted to increase the polyphenol content of malted barley by the irradiation light source wavelength of the malted barley further comprising: the illumination intensity of the blue light and the red light is 1: 1.
3. the method for increasing the polyphenol content in malted barley according to claim 1 or 2, wherein the irradiation light source wavelength is adjusted to increase the polyphenol content in malted barley, and the method comprises the steps of: also comprises selecting full and intact barley, and soaking in sodium hypochlorite solution for sterilization before soaking barley to make barley absorb water.
4. The method of increasing the polyphenol content of malted barley as claimed in claim 3 wherein the irradiation light source wavelength is adjusted to increase the polyphenol content of malted barley as follows: the mass concentration of the sodium hypochlorite solution is 0.05%.
5. The method of increasing the polyphenol content of malted barley as claimed in claim 3 wherein the irradiation light source wavelength is adjusted to increase the polyphenol content of malted barley as follows: and (4) sterilizing for 20-40 min.
6. The method of increasing the polyphenol content of malted barley as claimed in claim 3 wherein the irradiation light source wavelength is adjusted to increase the polyphenol content of malted barley as follows: also comprises washing the sterilized barley with water.
7. The method of increasing the polyphenol content of malted barley as claimed in claim 6 wherein the irradiation light source wavelength is adjusted to increase the polyphenol content of malted barley by the irradiation light source wavelength of the malted barley further comprising: the barley is soaked to make the barley absorb water, and the soaking temperature is room temperature.
8. The method for increasing the polyphenol content in malted barley according to claim 1 or 2, wherein the irradiation light source wavelength is adjusted to increase the polyphenol content in malted barley, and the method comprises the steps of: and the germination is realized by flatly paving the barley on a container filled with gauze for germination, wherein the germination time is 72-96 hours.
9. The method of increasing the polyphenol content of malted barley as claimed in claim 8 wherein the irradiation light source wavelength is adjusted to increase the polyphenol content of malted barley by: and also comprises sprinkling water every 24h in the germination process, and treating the box body with ozone.
10. The method of increasing the polyphenol content of malted barley as claimed in claim 9 wherein the irradiation light source wavelength is adjusted to increase the polyphenol content of malted barley by: and during germination, the germination temperature is controlled to be 25 ℃, and the relative humidity is controlled to be 90%.
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2019
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| CN101828527B (en) * | 2010-05-21 | 2011-12-07 | 南京农业大学 | Method for controlling quick propagation light source for upland cotton |
| CN105766338A (en) * | 2016-03-30 | 2016-07-20 | 安徽科技学院 | Seedling culture method of Abelmoschus esculentus |
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| CN107535118A (en) * | 2017-10-26 | 2018-01-05 | 上海交通大学 | A kind of preparation technology for improving black barley germination efficiency, active component content and antioxidation activity |
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