CN110894465A - KM mouse embryo body is cultivateed outward and is used viewing device - Google Patents

KM mouse embryo body is cultivateed outward and is used viewing device Download PDF

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Publication number
CN110894465A
CN110894465A CN201911312388.8A CN201911312388A CN110894465A CN 110894465 A CN110894465 A CN 110894465A CN 201911312388 A CN201911312388 A CN 201911312388A CN 110894465 A CN110894465 A CN 110894465A
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observation
welded
box
observation box
top end
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张晋平
丁艳平
王俊龙
杨宁
张继
孔维宝
朱学泰
武国凡
傅龙飞
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Northwest Normal University
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/22Transparent or translucent parts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/24Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48Automatic or computerized control

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Abstract

The invention relates to the technical field of KM mouse embryo culture, in particular to an observation device for KM mouse embryo body external culture, which comprises an observation box, wherein an observation mirror is installed at the top end of the observation box, first support rods are welded at four corners of the bottom end of the observation box, a fixed block is clamped at the top end of each first support rod, a first clamping groove matched with each first support rod is formed in the bottom end of each fixed block, a placing plate is welded at the top end of each fixed block, a second clamping groove is uniformly formed in the top end of each placing plate, a first clamping block is clamped in each second clamping groove, a sterilization protection pad is bonded at the top end of each first clamping block, switch doors are hinged to the wall bodies on the periphery of the observation box above the placing plates, and an observation window is installed in. The KM mouse holder can be conveniently detached, so that the KM mouse holder can be conveniently cleaned, and due to the arrangement of the observation windows and the observation mirrors, a KM mouse can be observed comprehensively.

Description

KM mouse embryo body is cultivateed outward and is used viewing device
Technical Field
The invention relates to the technical field of KM mouse embryo culture, in particular to an observation device for KM mouse embryo body in-vitro culture.
Background
The KM mice are outcrossing group mice with the largest output and use amount in China. The gene bank is large, the gene heterozygosis rate is high, and Chinese scholars have successively cultivated a plurality of inbred line mice from KM mouse inbred groups. After years of breeding, the KM mice have extremely low spontaneous tumor rate. There is a certain difference in the growth development and reproductive performance of the enclosed population of Kunming mice bred differently. But has the common characteristics of strong disease resistance and adaptability, high reproduction rate and high survival rate.
The KM mouse embryo among the current observation device is placed to KM mouse embryo body external culture all welds in the observation box to inconvenient dismantlement gets off to clean, and current observation device only has a side observation window, observation KM mouse that can not the full aspect.
Disclosure of Invention
The invention aims to solve the defects that a placing plate is inconvenient to detach for cleaning and a KM mouse cannot be observed comprehensively in the prior art, and provides an observation device for culturing KM mouse embryo bodies.
In order to achieve the purpose, the invention adopts the following technical scheme:
the observation device for KM mouse embryo body external culture comprises an observation box, wherein an observation mirror is installed at the top end of the observation box, first supporting rods are welded at four corners of the bottom end of the observation box, a fixed block is clamped at the top end of each first supporting rod, a first clamping groove matched with the first supporting rods is formed in the bottom end of each fixed block, a placing plate is welded at the top end of each fixed block, second clamping grooves are uniformly formed in the top end of the placing plate, first clamping blocks are clamped in the second clamping grooves, sterilization protection pads are bonded at the top ends of the first clamping blocks, switch doors are hinged to the peripheral wall bodies of the observation box above the placing plate, an observation window is installed in the middle of each switch door, shading cloth is bonded to the outer side of each switch door above the observation window, placing rods matched with the shading cloth are welded to the outer side of the observation box above the switch doors, the outside below of switch door is provided with block mechanism, temperature sensor is installed on the inside top of observation box, single chip microcomputer controller is installed on the outside top of observation box, temperature sensor, single chip microcomputer controller all are connected with external power source through the wire, temperature sensor's control output end is connected with single chip microcomputer controller's control input end, temperature regulation mechanism is installed to the bottom of observation box below that is located the board of placing, supporting mechanism is installed to the bottom of observation box, the bottom mid-mounting of observation box has fixed establishment.
Preferably, temperature regulation mechanism installs the hot plate including installing bottom one side at the observation box, even installation has the heating pipe in the hot plate, the box has been placed to the opposite side of observation box bottom, install the refrigerator in the box, the aspirator pump is installed to one side of box, the pipeline is installed to the end of giving vent to anger of aspirator pump, the even shower nozzle of installing in top of pipeline, heating pipe, refrigerator, aspirator pump all are connected with external power source through the wire, and the control input of heating pipe, refrigerator, aspirator pump is connected with single chip microcomputer controller's control output.
Preferably, the clamping mechanism comprises a connecting plate welded on the lower portion of the outer side of the switch door, a third clamping groove is formed in one side of the connecting plate, a second clamping block is clamped in the third clamping groove, and one side of the second clamping block is welded on the wall body of the observation box.
Preferably, the supporting mechanism includes the first supporting leg of welding in observation case bottom end four corners, damping spring has been welded to the bottom of first supporting leg, damping spring's one end welding has the second supporting leg, the bottom welding of second supporting leg has the universal wheel, install coupling mechanism between first supporting leg and the second supporting leg.
Preferably, coupling mechanism includes that the slidable cup joints the loop bar in first supporting leg below, the spout with first supporting leg matched with is seted up at the middle part of loop bar, the top sliding connection of second supporting leg is in the spout at loop bar middle part, damping spring between first supporting leg and the second supporting leg is located in the spout at loop bar middle part.
Preferably, the diameters of the first supporting leg and the second supporting leg are equal, and the first supporting leg, the second supporting leg and the loop bar are concentric.
Preferably, the fixing mechanism comprises fixing rods symmetrically welded in the middle of the bottom end of the observation box, a second supporting rod is connected below the fixing rods through a rotating mechanism, a friction plate is welded at the bottom end of the second supporting rod, and an anti-slip pad is bonded at the bottom end of the friction plate.
Preferably, the rotating mechanism comprises a rotating groove formed below the fixing rod, a connecting shaft is rotatably connected in the rotating groove, and a second supporting rod is welded at one end of the connecting shaft.
The observation device for the in-vitro culture of the KM mouse embryo body has the beneficial effects that:
1. the observation box, place board, fixed block, the cooperation setting between first bracing piece and the first draw-in groove, can conveniently dismantle and place the board to the board is placed to convenient regular cleaning, gives the clean sanitary living environment of KM mouse embryo, and the setting of the protection pad that disinfects can protect KM mouse embryo, and the bacterium is exterminateed to help KM mouse embryo, and the cooperation setting between second draw-in groove, first fixture block and the protection pad that disinfects can conveniently be changed the protection pad that disinfects.
2. The cooperation setting between observation box, sight glass, switch door and the observation window, observation KM mouse that can the full aspect to can make things convenient for the growth condition of staff record KM mouse embryo more, the shading cloth with place the cooperation between the pole, can shelter from when not needing light, can make things convenient for the fixed switch door in the cooperation setting between connecting plate, third draw-in groove and the second fixture block.
3. The heating plate, the heating pipe, the box body, the refrigerator, the air suction pump, the pipeline, the spray head, the temperature sensor and the single chip microcomputer controller are matched, so that the temperature in the observation box can be adjusted, and the production temperature of the KM mouse embryos can be guaranteed.
Drawings
FIG. 1 is a schematic front view of an observation apparatus for in vitro culture of KM mouse embryo according to the present invention;
FIG. 2 is a schematic sectional structure view of a KM mouse embryo in-vitro culture observation device according to the present invention;
fig. 3 is a schematic left sectional structure view of an observation apparatus for in vitro culture of KM mouse embryo according to the present invention.
In the figure: the observation box 1, the observation mirror 2, place board 3, fixed block 4, first bracing piece 5, first draw-in groove 6, second draw-in groove 7, first fixture block 8, the protection pad 9 that disinfects, switch door 10, observation window 11, shading cloth 12, place pole 13, connecting plate 14, third draw-in groove 15, second fixture block 16, hot plate 17, heating pipe 18, box 19, refrigerator 20, aspirator pump 21, pipeline 22, shower nozzle 23, temperature sensor 24, singlechip controller 25, first supporting leg 26, damping spring 27, loop bar 28, second supporting leg 29, universal wheel 30, dead lever 31, rotation groove 32, connecting axle 33, second bracing piece 34, friction plate 35, slipmat 36.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Example 1
Referring to fig. 1-3, an observation device for KM mouse embryo body external culture comprises an observation box 1, an observation mirror 2 is installed at the top end of the observation box 1, first support rods 5 are welded at four corners of the bottom end of the observation box 1, a fixed block 4 is clamped at the top end of each first support rod 5, a first clamping groove 6 matched with each first support rod 5 is formed at the bottom end of each fixed block 4, a placing plate 3 is welded at the top end of each fixed block 4, a second clamping groove 7 is uniformly formed at the top end of each placing plate 3, a first clamping block 8 is clamped in each second clamping groove 7, a sterilization protection pad 9 is bonded at the top end of each first clamping block 8, the observation box 1, the placing plate 3, the fixed blocks 4, the first support rods 5 and the first clamping grooves 6 are matched, the placing plate 3 can be conveniently detached regularly, the placing plate 3 is convenient to clean, a clean and sanitary living environment is provided for KM mouse embryos, and the sterilization protection pad 9 is, can protect KM mouse embryo, help KM mouse embryo exterminate the bacterium, the protection pad 9 that disinfects can conveniently be changed to cooperation setting between second draw-in groove 7, first fixture block 8 and the protection pad 9 that disinfects.
The utility model discloses a KM mouse, including observation box 1, observation box 3, switch door 10, observation window 11, shading cloth 12, placing rod 13, clamping mechanism, connecting plate 14, third clamping groove 15, second clamping block 16, observing box 1, observation mirror 2, cooperation setting between switch door 10 and observation window 11, can be used for observing KM mouse in all aspects, thereby can be more convenient for the staff to record the growth condition of KM mouse embryo, the cooperation between shading cloth 12 and placing rod 13, can shelter from light when not needing light, the cooperation setting between connecting plate 14, third draw-in groove 15 and the second fixture block 16 can make things convenient for the fixed switch door.
A temperature sensor 24 is arranged at the top end inside the observation box 1, a single chip microcomputer controller 25 is arranged at the top end outside the observation box 1, the temperature sensor 24 and the single chip microcomputer controller 25 are both connected with an external power supply through leads, the control output end of the temperature sensor 24 is connected with the control input end of the single chip microcomputer controller 25, a temperature adjusting mechanism is arranged at the bottom end of the observation box 1 below the placing plate 3, the temperature adjusting mechanism comprises a heating plate 17 arranged at one side of the bottom end of the observation box 1, heating pipes 18 are uniformly arranged in the heating plate 17, a box body 19 is arranged at the other side of the bottom end of the observation box 1, a refrigerator 20 is arranged in the box body 19, an air suction pump 21 is arranged at one side of the box body 19, a pipeline 22 is arranged at the air outlet end of the air suction pump 21, spray heads 23 are uniformly arranged at the top end, and the control input end of the heating pipe 18, the refrigerator 20 and the air suction pump 21 is connected with the control output end of the single chip microcomputer controller 25, the bottom end of the observation box 1 is provided with a supporting mechanism, the middle part of the bottom end of the observation box 1 is provided with a fixing mechanism, the heating plate 17, the heating pipe 18, the box body 19, the refrigerator 20, the air suction pump 21, the pipeline 22, the spray head 23, the temperature sensor 24 and the single chip microcomputer controller 25 are arranged in a matching way, the temperature in the observation box 1 can be adjusted, so that the production temperature of the KM mouse embryo can be ensured, when the temperature sensor 24 detects that the temperature is too low, a signal is transmitted to the single chip microcomputer controller 25, the single chip microcomputer controller 25 controls the heating pipe 18 to carry out heating work, when the temperature rises to a certain value, the heating is stopped, when the temperature sensor 24 detects that the temperature is too high, the signal is, the cold air generated by refrigerator 20 is pumped out of box 19 by controlling suction pump 21, and then is discharged into observation box 1 through head 23.
Example 2
Referring to fig. 1-3, as another preferred embodiment of the present invention, the difference from embodiment 1 is that the supporting mechanism includes first supporting legs 26 welded at four corners of the bottom end of the observation box 1, a damping spring 27 is welded at the bottom end of the first supporting leg 26, a second supporting leg 29 is welded at one end of the damping spring 27, a universal wheel 30 is welded at the bottom end of the second supporting leg 29, a connecting mechanism is installed between the first supporting leg 26 and the second supporting leg 29, the connecting mechanism includes a loop bar 28 slidably sleeved under the first supporting leg 26, a sliding slot matched with the first supporting leg 26 is formed in the middle of the loop bar 28, the top end of the second supporting leg 29 is slidably connected in the sliding slot in the middle of the loop bar 28, the damping spring 27 between the first supporting leg 26 and the second supporting leg 29 is located in the sliding slot in the middle of the loop bar 28, the diameters of the first supporting leg 26 and the second supporting leg 29 are equal, and first supporting leg 26, second supporting leg 29 and loop bar 28 center line altogether, the cooperation setting between first supporting leg 26, damping spring 27, loop bar 28 and the second supporting leg 29 can conveniently support observation box 1, and can also the shock attenuation, and observation box 1 is conveniently removed in the setting of universal wheel 30.
Example 3
Referring to fig. 1-3, as another preferred embodiment of the present invention, the difference from embodiment 1 is that the fixing mechanism includes a fixing rod 31 symmetrically welded at the middle of the bottom end of the observation box 1, a second support rod 34 is connected below the fixing rod 31 through a rotating mechanism, the rotating mechanism includes a rotating groove 32 arranged below the fixing rod 31, a connecting shaft 33 is rotatably connected in the rotating groove 32, one end of the connecting shaft 33 is welded with the second support rod 34, the bottom end of the second support rod 34 is welded with a friction plate 35, the bottom end of the friction plate 35 is bonded with an anti-slip pad 36, and the fixing rod 31, the rotating groove 32, the connecting shaft 33, the second support rod 34 and the friction plate 35 are cooperatively arranged, so that the observation box 1 can be conveniently fixed, the observation box 1 is prevented from moving, the anti-slip pad 36 is arranged, and friction.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (8)

1. An observation device for KM mouse embryoid body external culture comprises an observation box (1) and is characterized in that an observation mirror (2) is installed at the top end of the observation box (1), first supporting rods (5) are welded at four corners of the bottom end of the observation box (1), fixed blocks (4) are clamped at the top ends of the first supporting rods (5), first clamping grooves (6) matched with the first supporting rods (5) are formed in the bottom ends of the fixed blocks (4), a placing plate (3) is welded at the top end of the fixed blocks (4), second clamping grooves (7) are uniformly formed in the top end of the placing plate (3), first clamping blocks (8) are clamped in the second clamping grooves (7), a sterilization protection pad (9) is bonded at the top end of each first clamping block (8), and hinge switch doors (10) are connected to the peripheral wall bodies of the observation box (1) above the placing plate (3), the switch door is characterized in that an observation window (11) is installed in the middle of the switch door (10), shading cloth (12) is bonded above the observation window (11) on the outer side of the switch door (10), a placing rod (13) matched with the shading cloth (12) is welded on the upper side of the switch door (10) on the outer side of the observation box (1), a clamping mechanism is arranged below the outer side of the switch door (10), a temperature sensor (24) is installed at the top end of the inner part of the observation box (1), a single chip microcomputer controller (25) is installed at the top end of the outer side of the observation box (1), the temperature sensor (24) and the single chip microcomputer controller (25) are connected with an external power supply through wires, the control output end of the temperature sensor (24) is connected with the control input end of the single chip microcomputer controller (25), and a temperature adjusting mechanism is installed below the placing plate (3) on the bottom end of the observation box, supporting mechanism is installed to the bottom of observation box (1), the bottom mid-mounting of observation box (1) has fixed establishment.
2. The observation apparatus for in vitro culture of KM mouse embryos according to claim 1, the temperature adjusting mechanism comprises a heating plate (17) arranged at one side of the bottom end of the observation box (1), heating pipes (18) are uniformly arranged in the heating plate (17), a box body (19) is arranged on the other side of the bottom end of the observation box (1), a refrigerator (20) is arranged in the box body (19), a suction pump (21) is arranged on one side of the box body (19), a pipeline (22) is arranged at the air outlet end of the air suction pump (21), spray heads (23) are uniformly arranged at the top end of the pipeline (22), the heating pipe (18), the refrigerator (20) and the air suction pump (21) are all connected with an external power supply through leads, and the control input ends of the heating pipe (18), the refrigerator (20) and the air suction pump (21) are connected with the control output end of the singlechip controller (25).
3. The observation device for KM mouse embryo in-vitro culture according to claim 1, wherein the engaging mechanism comprises a connecting plate (14) welded below the outer side of the switch door (10), a third engaging groove (15) is formed on one side of the connecting plate (14), a second engaging block (16) is engaged in the third engaging groove (15), and one side of the second engaging block (16) is welded on the wall body of the observation box (1).
4. The observation device for KM mouse embryo body external culture according to claim 1, wherein the supporting mechanism comprises a first supporting leg (26) welded at four corners of the bottom end of the observation box (1), a damping spring (27) is welded at the bottom end of the first supporting leg (26), a second supporting leg (29) is welded at one end of the damping spring (27), a universal wheel (30) is welded at the bottom end of the second supporting leg (29), and a connecting mechanism is installed between the first supporting leg (26) and the second supporting leg (29).
5. The observation device for KM mouse embryo body in-vitro culture according to claim 4, wherein the connection mechanism comprises a loop bar (28) slidably sleeved under the first support leg (26), the middle part of the loop bar (28) is provided with a sliding groove matched with the first support leg (26), the top end of the second support leg (29) is slidably connected in the sliding groove at the middle part of the loop bar (28), and the damping spring (27) between the first support leg (26) and the second support leg (29) is positioned in the sliding groove at the middle part of the loop bar (28).
6. The observation apparatus for culturing a KM mouse embryo in vitro, according to claim 5, wherein the first support leg (26) and the second support leg (29) have the same diameter, and the first support leg (26), the second support leg (29) and the loop bar (28) are concentric.
7. The observation device for KM mouse embryo in-vitro culture according to claim 1, wherein the fixing mechanism comprises a fixing rod (31) symmetrically welded at the middle part of the bottom end of the observation box (1), a second support rod (34) is connected below the fixing rod (31) through a rotating mechanism, a friction plate (35) is welded at the bottom end of the second support rod (34), and a non-slip pad (36) is bonded at the bottom end of the friction plate (35).
8. The observation device for the in-vitro culture of the KM mouse embryo body as claimed in claim 7, wherein the rotation mechanism comprises a rotation groove (32) formed below the fixing rod (31), a connecting shaft (33) is rotatably connected in the rotation groove (32), and a second support rod (34) is welded at one end of the connecting shaft (33).
CN201911312388.8A 2019-12-18 2019-12-18 KM mouse embryo body is cultivateed outward and is used viewing device Pending CN110894465A (en)

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CN208055365U (en) * 2018-03-16 2018-11-06 广东医科大学附属医院 A kind of zebrafish embryo incubator rapidly and efficiently
CN108799779A (en) * 2018-07-02 2018-11-13 苏州驰夫电子科技有限公司 A kind of computer fittings lifting type support
CN208768625U (en) * 2018-08-06 2019-04-23 江苏紫山生物股份有限公司 A kind of edible fungi inoculation box
CN209236549U (en) * 2018-10-10 2019-08-13 西安交通大学医学院第一附属医院 With clammy deposited and applying hot soaks function limbs special for treating device
CN209065913U (en) * 2018-10-15 2019-07-05 广州贝特生物科技有限公司 A kind of variable ratio frequency changer insulating box
CN209745817U (en) * 2018-11-28 2019-12-06 安徽省农业科学院园艺研究所 Seedling substrate pH value real-time detection device
CN110317730A (en) * 2019-06-19 2019-10-11 禹州市龙跃牧业有限公司 One kind is for the outer culture apparatus of bovine embryo
CN210765336U (en) * 2019-09-10 2020-06-16 深圳天溯计量检测股份有限公司 Incubator convenient to observe for microbiological experiment

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Application publication date: 20200320