CN110903975A - Adjustable cell culture tray placing frame and adjusting method - Google Patents

Adjustable cell culture tray placing frame and adjusting method Download PDF

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Publication number
CN110903975A
CN110903975A CN201911293034.3A CN201911293034A CN110903975A CN 110903975 A CN110903975 A CN 110903975A CN 201911293034 A CN201911293034 A CN 201911293034A CN 110903975 A CN110903975 A CN 110903975A
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cell culture
fixedly connected
frame
pipe
sliding sleeve
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陈志勇
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/46Means for fastening
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature

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Abstract

The invention discloses an adjustable cell culture tray placing frame and an adjusting method, wherein the adjustable cell culture tray placing frame comprises a bottom plate, fixing columns are fixedly connected to two sides of the top of the bottom plate, a sliding sleeve is sleeved on the surface of each fixing column, a threaded sleeve is embedded in one side opposite to the sliding sleeve, a threaded rod penetrates through the inner cavity of the threaded sleeve, and one side opposite to the threaded rod penetrates through the inner cavity of the sliding sleeve. According to the invention, through the matched use of the adjusting frame, the fixed column, the telescopic hose, the top plate, the collecting pipe, the air pump, the steam generator, the placing box, the air outlet pipe, the moving frame, the threaded sleeve, the sliding sleeve, the threaded rod, the telescopic spring and the clamping plate, the problems that the existing cell culture tray placing frame cannot be adjusted according to the size of a culture tray in the process of placing the culture tray, so that the cell culture tray placing frame cannot adapt to culture trays with different sizes, and meanwhile, the placing space of the cell culture tray placing frame cannot be adjusted, unnecessary space is occupied in the using process, and space waste is caused are solved.

Description

Adjustable cell culture tray placing frame and adjusting method
Technical Field
The invention relates to the technical field of cell culture, in particular to an adjustable cell culture tray placing frame and an adjusting method.
Background
Cell culture refers to a method for simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain main structure and function, and is also called cell cloning technology, the formal term in biology is cell culture technology, the cell culture is an indispensable process for the whole bioengineering technology and one of the biological cloning technologies, the cell culture is the large-scale cloning of the cells, the cell culture technology can be used for culturing a large amount of cells into simple single cells or minimally differentiated multiple cells, which is an indispensable link of the cloning technology, the cell culture is the cloning of the cells, the cell culture technology is an important and common technology in the research method of cell biology, and a large amount of cells can be obtained through cell culture, further, signal transduction of cells, anabolism of cells, growth and proliferation of cells, and the like can be studied.
Need use the cell culture dish at the in-process of cell culture, and need use the rack at the in-process of placing the cell culture dish, current cell culture dish place the rack at the in-process of placing the culture dish, can't adjust according to the size of cultivateing the dish to unable adaptation not equidimension culture dish, simultaneously, can't place the space to it and adjust, at the in-process of using, can occupy unnecessary space, caused the waste in space.
Disclosure of Invention
The invention aims to provide an adjustable cell culture tray placing frame which has the advantage of being adjustable and solves the problems that the existing cell culture tray placing frame cannot be adjusted according to the size of a culture tray in the process of placing the culture tray, so that the cell culture tray placing frame cannot adapt to culture trays with different sizes, and meanwhile, the placing space of the cell culture tray placing frame cannot be adjusted, and unnecessary space is occupied in the using process, so that space is wasted.
In order to achieve the purpose, the invention provides the following technical scheme: an adjustable cell culture tray placing frame comprises a bottom plate, wherein fixed columns are fixedly connected to two sides of the top of the bottom plate, a sliding sleeve is sleeved on the surface of each fixed column, a threaded sleeve is embedded on one side opposite to the sliding sleeve, a threaded rod penetrates through the inner cavity of the threaded sleeve, one side opposite to the threaded rod penetrates into the inner cavity of the sliding sleeve, an adjusting frame is fixedly connected to one side opposite to the sliding sleeve, a moving frame is arranged in the inner cavity of the adjusting frame, a temperature sensor is fixedly connected to the front side of the center of the top of the inner cavity of the moving frame, a bubble level meter is fixedly connected to the center of the bottom of the front surface of the moving frame, a placing box is fixedly connected to the bottom of the inner cavity of the moving frame, telescopic springs are fixedly connected to two sides of the inner cavity of the placing box, clamping plates are, the utility model discloses a steam generator, including roof, air extracting pump, intake pipe, frame, collecting pipe, manifold, the bottom intercommunication of lateral duct has the outlet duct, the left side fixedly connected with steam generator at roof top, the center department fixedly connected with aspiration pump at roof top, the inlet end intercommunication of aspiration pump has the intake pipe, the one end that the aspiration pump was kept away from in the intake pipe communicates in steam generator's the end of giving vent to anger, the end intercommunication of giving vent to anger of aspiration pump has the standpipe, the bottom of standpipe run through in proper order remove the frame and adjust the frame and with adjust frame fixed connection, the both sides of standpipe and be located the top of removing the frame and all communicate and have violently the pipe, the one end intercommunication that violently keeps away from the standpipe is violently managed.
Preferably, the four corners of the bottom plate are fixedly connected with supporting legs, the bottom of each supporting leg is fixedly connected with a supporting seat, and anti-skid grains are arranged at the bottom of each supporting seat.
Preferably, one side of the threaded rod, which is located in the inner cavity of the sliding sleeve, is fixedly connected with a first rubber pad, the inner cavity of the sliding sleeve is connected with the surface of the fixing column in a sliding manner, and the opposite side of the threaded rod penetrates through the outer side of the sliding sleeve and is fixedly connected with a fastening knob.
Preferably, the sliding grooves are formed in the two sides of the bottom of the inner cavity of the adjusting frame, the sliding blocks are fixedly connected to the two sides of the bottom of the moving frame and located right above the sliding grooves, and the bottoms of the sliding blocks extend to the inner cavity of the sliding grooves and are connected with the inner wall of the sliding blocks in a sliding mode.
Preferably, the clamping plate is of an arc-shaped structure, a second rubber pad is fixedly connected to the inner wall of the clamping plate, and the outer wall of the second rubber pad is fixedly connected with the inner wall of the clamping plate.
Preferably, the center of the top of the movable frame is provided with a groove, the number of the placing boxes is twelve, and six placing boxes are arranged on two sides of the inner cavity of the movable frame respectively.
Preferably, the number of the branch pipes is three, the branch pipes are evenly distributed on one side of the collecting pipe, one end, far away from the collecting pipe, of each branch pipe is fixedly connected with a fixing frame, and one end, far away from the branch pipes, of each fixing frame is fixedly connected to one side of the top of the adjusting frame.
Preferably, the number of adjusting the frame is three at least, and the specification is the same, the through-hole that uses with the standpipe cooperation is seted up in the center department at roof top, the center department fixedly connected with pull handle at removal frame positive surface top.
Preferably, the method for adjusting the adjustable cell culture dish placing rack comprises the following steps:
a: placing the cell culture disc between the clamping plates in the placing box, then compressing the expansion spring according to the size of the cell culture disc, then loosening the expansion spring after placing the expansion spring, and enabling the expansion spring to rebound, so that the clamping plates are driven to move, and the cell culture disc is clamped by the clamping plates, thereby achieving the fixing effect;
b: then put the moving frame into the regulating frame, make the slide block of its bottom draw into the concrete chute, then according to the required height, move the regulating frame up and down, make the sliding sleeve slide on the surface of the fixed column, after it regulates to the appropriate position, rotate the fastening knob, make the threaded rod rotate, thus make its rubber pad one of one side contact with surface of the fixed column closely, realize the fixation to the sliding sleeve, thus reach the fixed effect to the regulating frame, observe the bubble spirit level, judge whether its bubble is horizontal, if not horizontal, can finely tune the sliding sleeve of both sides up and down, make it in the horizontal state;
c: in the process of moving the adjusting frame, the vertical pipe is driven to move, so that the horizontal pipe, the collecting pipe, the branch pipe and the air outlet pipe are driven to move in sequence, when the vertical pipe moves up and down, the telescopic hose is also lengthened or shortened, and when the vertical pipe needs to be disinfected, the steam generator and the air pump are started to pump external air in through the steam generator, then generating steam, entering the air inlet pipe through the air outlet end, and spraying the steam in the air inlet pipe to the placing box through the vertical pipe, the telescopic hose, the transverse pipe, the collecting pipe, the branch pipe and the air outlet pipe in sequence by the air pump, thereby sterilizing and disinfecting the bacteria culture disc, avoiding pollution when culturing cells, presetting the detection value of the temperature sensor, detecting the temperature in the movable frame through the temperature sensor, when the temperature is higher than the detection temperature, the steam generator and the air pump stop working to reduce the temperature.
Compared with the prior art, the invention has the following beneficial effects:
1. according to the invention, through the matched use of the adjusting frame, the fixed column, the telescopic hose, the top plate, the collecting pipe, the air pump, the steam generator, the placing box, the air outlet pipe, the moving frame, the threaded sleeve, the sliding sleeve, the threaded rod, the telescopic spring and the clamping plate, the placing space of the culture tray can be adjusted, and meanwhile, the culture tray can be adjusted up and down, so that the space waste caused by the occupation of excessive space is avoided, and the problems that the existing cell culture tray placing frame cannot be adjusted according to the size of the culture tray in the placing process of the culture tray, so that the culture trays with different sizes cannot be adapted, and meanwhile, the placing space of the existing cell culture tray placing frame cannot be adjusted, and unnecessary space is occupied in the using process, so that the space waste is caused are solved.
2. The invention can stably support the support frame by using the support legs, can conveniently rotate the threaded rod by using the fastening knob, can conveniently fix the sliding sleeve by using the threaded rod and the threaded sleeve, can adjust the position of the adjusting frame by using the sliding sleeve, can conveniently take out and put in the adjusting frame by using the sliding chute and the sliding block, can support the branch pipe by using the fixing frame to avoid shaking in the moving process, can stretch along with the vertical pipe in the moving process by using the telescopic hose to ensure that the vertical pipe is always communicated with the vertical pipe, can observe whether the moving frame is in a horizontal state by using the bubble level gauge, avoids the condition that the culture tray shakes due to the inclination of the moving frame, and can conveniently take out the moving frame by using the drawing handle, through temperature sensor's use, can detect the temperature in the removal frame, avoid its high temperature and cause the injury to cultivateing the dish.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is an enlarged view of A of FIG. 1 according to the present invention;
FIG. 3 is an enlarged view of B of FIG. 1 according to the present invention;
FIG. 4 is a schematic view of a moving frame of the present invention;
FIG. 5 is a top view of the movable frame of the present invention;
FIG. 6 is a top view of the manifold of the present invention.
In the figure: the device comprises a base plate 1, an adjusting frame 2, a sliding chute 3, a fixing column 4, a fixing frame 5, an expansion hose 6, a top plate 7, a collecting pipe 8, an air pump 9, a vertical pipe 10, a horizontal pipe 11, an air inlet pipe 12, a steam generator 13, a box for placing 14, an air outlet pipe 15, a branch pipe 16, a fastening knob 17, a supporting leg 18, a sliding block 19, a movable frame 20, a threaded sleeve 21, a sliding sleeve 22, a threaded rod 23, an expansion spring 24, a clamping plate 25, a bubble level meter 26, a groove 27 and a temperature sensor 28.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front", "rear", "both ends", "one end", "the other end", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be configured in a specific orientation, and operate, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "connected," and the like are to be construed broadly, such as "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The bottom plate 1, the adjusting frame 2, the fixing column 4, the fixing frame 5, the telescopic hose 6, the top plate 7, the collecting pipe 8, the air pump 9, the vertical pipe 10, the horizontal pipe 11, the air inlet pipe 12, the steam generator 13, the placing box 14, the air outlet pipe 15, the branch pipe 16, the fastening knob 17, the supporting leg 18, the sliding block 19, the moving frame 20, the threaded sleeve 21, the sliding sleeve 22, the threaded rod 23, the telescopic spring 24, the clamping plate 25, the bubble level gauge 26 and the temperature sensor 28 are all universal standard parts or parts known by a person skilled in the art, and the structure and the principle of the device can be known by a technical manual or a conventional experimental method.
Referring to fig. 1-6, an adjustable cell culture tray placing frame comprises a bottom plate 1, wherein both sides of the top of the bottom plate 1 are fixedly connected with fixed columns 4, the surface of each fixed column 4 is sleeved with a sliding sleeve 22, the position of an adjusting frame 2 can be adjusted by using the sliding sleeve 22, one side of the sliding sleeve 22 opposite to the sliding sleeve 22 is embedded with a threaded sleeve 21, an inner cavity of the threaded sleeve 21 is provided with a threaded rod 23 in a penetrating manner, one side of the threaded rod 23 opposite to the inner cavity of the sliding sleeve 22 is provided with the adjusting frame 2, the inner cavity of the adjusting frame 2 is provided with a moving frame 20, the front side of the center of the top of the inner cavity of the moving frame 20 is fixedly connected with a temperature sensor 28, the temperature in the moving frame 20 can be detected by using the temperature sensor 28, the damage to a culture tray caused by overhigh temperature can be avoided, the center of the bottom of, through the use of the bubble level meter 26, whether the movable frame 20 is in a horizontal state can be observed, the situation that the culture tray shakes due to the inclination of the movable frame is avoided, the bottom of the inner cavity of the movable frame 20 is fixedly connected with the placing box 14, both sides of the inner cavity of the placing box 14 are fixedly connected with the telescopic springs 24, one side opposite to the telescopic springs 24 is fixedly connected with the clamping plate 25, the top of the fixed column 4 is fixedly connected with the top plate 7, the left side of the top plate 7 is fixedly connected with the steam generator 13, the center of the top plate 7 is fixedly connected with the air suction pump 9, the air inlet end of the air suction pump 9 is communicated with the air outlet end of the steam generator 13, the air outlet end of the air suction pump 9 is communicated with the vertical pipe 10, the bottom of the vertical pipe 10 sequentially penetrates through the movable frame 20 and the adjusting frame 2 and is fixedly connected with the adjusting frame 2, both sides, the end, far away from the vertical pipe 10, of the horizontal pipe 11 is communicated with a collecting pipe 8, the end, far away from the horizontal pipe 11, of the collecting pipe 8 is communicated with a branch pipe 16, the bottom of the branch pipe 16 is communicated with an air outlet pipe 15, an expansion hose 6 is communicated between the vertical pipes 10 and above the horizontal pipe 11, and the vertical pipes 10 can expand and contract along with the collecting pipe in the moving process through the expansion hose 6, so that the vertical pipes are always communicated with the vertical pipes 10;
supporting legs 18 are fixedly connected to four corners of the bottom plate 1, supporting seats are fixedly connected to the bottoms of the supporting legs 18, anti-skid grains are formed in the bottoms of the supporting seats, and the supporting frame can be stably supported through the supporting legs 18;
one side of the threaded rod 23, which is positioned in the inner cavity of the sliding sleeve 22, is fixedly connected with a rubber pad I, the inner cavity of the sliding sleeve 22 is in sliding connection with the surface of the fixed column 4, the side, opposite to the threaded rod 23, penetrates through the outer side of the sliding sleeve 22 and is fixedly connected with a fastening knob 17, the threaded rod 23 can be conveniently rotated through the use of the fastening knob 17, the sliding sleeve 22 can be conveniently fixed through the use of the threaded rod 23 and a threaded sleeve 21, and the inner cavity of the threaded sleeve 21 is in threaded connection with the surface of the;
the two sides of the bottom of the inner cavity of the adjusting frame 2 are both provided with sliding grooves 3, the two sides of the bottom of the moving frame 20 and the two sides of the bottom of the moving frame 20 are both fixedly connected with sliding blocks 19 which are positioned right above the sliding grooves 3, the bottoms of the sliding blocks 19 extend to the inner cavity of the sliding grooves 3 and are in sliding connection with the inner walls of the sliding grooves 3, and the moving frame 20 can be conveniently taken out of and put into the adjusting frame 2 through the use of the sliding grooves 3 and;
the clamping plate 25 is of an arc-shaped structure, a second rubber pad is fixedly connected to the inner wall of the clamping plate 25, and the outer wall of the second rubber pad is fixedly connected with the inner wall of the clamping plate 25;
a groove 27 is formed in the center of the top of the movable frame 20, twelve placing boxes 14 are provided, and six placing boxes are respectively arranged on two sides of the inner cavity of the movable frame 20;
the number of the branch pipes 16 is three, the branch pipes are uniformly distributed on one side of the collecting pipe 8, one ends, far away from the collecting pipe 8, of the branch pipes 16 are fixedly connected with the fixing frames 5, one ends, far away from the branch pipes 16, of the fixing frames 5 are fixedly connected to one side of the top of the adjusting frame 2, and the branch pipes 16 can be supported through the fixing frames 5, so that the branch pipes are prevented from shaking in the moving process;
the number of the adjusting frames 2 is at least three, the specifications are the same, a through hole matched with the vertical pipe 10 for use is formed in the center of the top plate 7, a drawing handle is fixedly connected to the center of the top of the front surface of the moving frame 20, and the moving frame 20 can be conveniently drawn out through the drawing handle;
through the matching use of the adjusting frame 2, the fixing column 4, the telescopic hose 6, the top plate 7, the collecting pipe 8, the air extracting pump 9, the steam generator 13, the placing box 14, the air outlet pipe 15, the moving frame 20, the thread bush 21, the sliding bush 22, the threaded rod 23, the telescopic spring 24 and the clamping plate 25, the placing space of the culture tray can be adjusted, and meanwhile the culture tray can be adjusted up and down, so that the space waste caused by the occupation of too much space is avoided, and the problems that the existing cell culture tray placing frame cannot be adjusted according to the size of the culture tray in the placing process of the culture tray, so that the culture trays with different sizes cannot be adapted, meanwhile, the placing space cannot be adjusted, unnecessary space is occupied in the using process, and the space waste is caused are solved;
an adjustable method for adjusting a cell culture tray placing rack comprises the following steps:
a: placing the cell culture disc between the clamping plates 25 in the placing box 14, then compressing the expansion spring 24 according to the size of the cell culture disc, then loosening the expansion spring 24 after placing the cell culture disc, and enabling the expansion spring 24 to rebound, so that the clamping plates 25 are driven to move, and the cell culture disc is clamped by the clamping plates to achieve a fixing effect;
b: then put the moving frame 20 into the adjusting frame 2, make the slide block 19 of its bottom draw into the concrete chute 3, then according to the required height, move the adjusting frame 2 up and down, make the sliding bush 22 slide on the surface of the fixed column 4, after it is adjusted to the appropriate position, rotate the fastening knob 17, make the threaded spindle 23 rotate, thus make the rubber pad one of its one side contact with surface of the fixed column 4 closely, realize the fixed to the sliding bush 22, thus achieve the fixed effect to the adjusting frame 2, observe the bubble spirit level 26, judge whether its bubble is horizontal, if not horizontal, can finely tune the sliding bush 22 of both sides up and down, make it in the horizontal state;
c: in the process of moving the adjusting frame 2, the vertical pipe 10 is driven to move, thereby the horizontal pipe 11, the collecting pipe 8, the branch pipe 16 and the air outlet pipe 15 are sequentially driven to move, in the process of moving the vertical pipe 10 up and down, the flexible hose 6 is also extended or shortened, when the disinfection is needed, the steam generator 13 and the air pump 9 are started, external air is pumped in through the steam generator 13, then steam is generated and enters the air inlet pipe 12 through the air outlet end, the steam in the air inlet pipe 12 passes through the vertical pipe 10, the flexible hose 6, the horizontal pipe 11, the collecting pipe 8, the branch pipe 16 and the air outlet pipe 15 in sequence through the air pump 9, thereby the bacteria culture tray is sterilized and disinfected, the pollution generated during the cell culture is avoided, the detection value of the temperature sensor 28 is preset, the temperature in the moving frame 20 is detected through the temperature sensor 28, when the temperature is higher than the detection temperature, the steam generator 13 and the air pump 9 stop working, and the temperature is reduced.
To sum up: this cell culture dish rack with adjustable, through the alignment jig 2, fixed column 4, bellows 6, roof 7, the manifold 8, aspiration pump 9, steam generator 13, place box 14, outlet duct 15, remove frame 20, thread bush 21, sliding sleeve 22, threaded rod 23, expanding spring 24 and grip block 25's cooperation is used, the in-process of placing culture dish has been solved to current cell culture dish rack, can't adjust according to the size of culture dish, thereby unable adaptation not equidimension culture dish, and simultaneously, can't place the space to it and adjust, at the in-process that uses, can occupy unnecessary space, the extravagant problem in space has been caused.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (9)

1. The utility model provides a cell culture dish rack with adjustable, includes bottom plate (1), its characterized in that: the improved structure is characterized in that fixed columns (4) are fixedly connected to two sides of the top of the bottom plate (1), a sliding sleeve (22) is sleeved on the surface of each fixed column (4), a threaded sleeve (21) is inlaid on one side opposite to the sliding sleeve (22), a threaded rod (23) penetrates through the inner cavity of the threaded sleeve (21), one side opposite to the threaded rod (23) penetrates through the inner cavity of the sliding sleeve (22), an adjusting frame (2) is fixedly connected to one side opposite to the sliding sleeve (22), a moving frame (20) is arranged in the inner cavity of the adjusting frame (2), a temperature sensor (28) is fixedly connected to the front side of the center of the top of the inner cavity of the moving frame (20), a bubble level meter (26) is fixedly connected to the center of the bottom of the front surface of the moving frame (20), a placing box (14) is fixedly connected to the bottom of the inner cavity of the moving frame (20), and extension, a clamping plate (25) is fixedly connected to one side opposite to the telescopic spring (24), a top plate (7) is fixedly connected to the top of the fixed column (4), a steam generator (13) is fixedly connected to the left side of the top plate (7), an air suction pump (9) is fixedly connected to the center of the top plate (7), an air inlet end of the air suction pump (9) is communicated with an air inlet pipe (12), one end, far away from the air suction pump (9), of the air inlet pipe (12) is communicated with an air outlet end of the steam generator (13), an air outlet end of the air suction pump (9) is communicated with a vertical pipe (10), the bottom of the vertical pipe (10) sequentially penetrates through the moving frame (20) and the adjusting frame (2) and is fixedly connected with the adjusting frame (2), transverse pipes (11) are communicated with two sides of the vertical pipe (10) and positioned above the moving frame (20), one end, far away from the vertical pipe (10), one end of the collecting pipe (8) far away from the transverse pipe (11) is communicated with a branch pipe (16), the bottom of the branch pipe (16) is communicated with an air outlet pipe (15), and a flexible hose (6) is communicated between the vertical pipes (10) and above the transverse pipe (11).
2. An adjustable cell culture dish holder according to claim 1, wherein: the anti-skidding bottom plate is characterized in that supporting legs (18) are fixedly connected to four corners of the bottom plate (1), supporting seats are fixedly connected to the bottoms of the supporting legs (18), and anti-skidding lines are formed in the bottoms of the supporting seats.
3. An adjustable cell culture dish holder according to claim 1, wherein: the threaded rod (23) is located one side of the inner cavity of the sliding sleeve (22) and is fixedly connected with a rubber pad I, the inner cavity of the sliding sleeve (22) is connected with the surface of the fixing column (4) in a sliding mode, and the opposite side of the threaded rod (23) penetrates through the outer side of the sliding sleeve (22) and is fixedly connected with a fastening knob (17).
4. An adjustable cell culture dish holder according to claim 1, wherein: spout (3) have all been seted up to the both sides of adjusting frame (2) inner chamber bottom, remove the both sides of frame (20) bottom and be located spout (3) equal fixedly connected with slider (19) directly over, the bottom of slider (19) extend to the inner chamber of spout (3) and rather than inner wall sliding connection.
5. An adjustable cell culture dish holder according to claim 1, wherein: the clamping plate (25) is of an arc-shaped structure, a second rubber pad is fixedly connected to the inner wall of the clamping plate (25), and the outer wall of the second rubber pad is fixedly connected with the inner wall of the clamping plate (25).
6. An adjustable cell culture dish holder according to claim 1, wherein: the center of the top of the movable frame (20) is provided with a groove (27), the number of the placing boxes (14) is twelve, and six placing boxes are positioned on two sides of the inner cavity of the movable frame (20).
7. An adjustable cell culture dish holder according to claim 1, wherein: the quantity of bleeder (16) is three, and evenly distributed in one side of collecting pipe (8), the one end fixedly connected with mount (5) of collecting pipe (8) are kept away from in bleeder (16), one end fixed connection in the one side at regulation frame (2) top that bleeder (16) were kept away from in mount (5).
8. An adjustable cell culture dish holder according to claim 1, wherein: the quantity of adjusting frame (2) is three at least, and the specification is the same, the through-hole that uses with standpipe (10) cooperation is seted up in the center department at roof (7) top, the center department fixedly connected with pull handle at removal frame (20) positive surface top.
9. The method for adjusting an adjustable cell culture dish rack according to claims 1-8, comprising the steps of:
a: placing the cell culture disc between clamping plates (25) in a placing box (14), compressing a telescopic spring (24) according to the size of the cell culture disc, then loosening the telescopic spring (24) after the cell culture disc is placed in the cell culture disc, and enabling the telescopic spring (24) to rebound, so that the clamping plates (25) are driven to move, the cell culture disc is clamped, and the fixing effect is achieved;
b: then the movable frame (20) is placed into the adjusting frame (2), a sliding block (19) at the bottom of the movable frame is made to slide into the sliding groove (3), then the adjusting frame (2) is moved up and down according to the required height, the sliding sleeve (22) is made to slide on the surface of the fixed column (4), after the sliding sleeve is adjusted to a proper position, the fastening knob (17) is rotated, the threaded rod (23) is made to rotate, the rubber pad I on one side of the threaded rod is made to be in close contact with the surface of the fixed column (4), the sliding sleeve (22) is fixed, the effect of fixing the adjusting frame (2) is achieved, the bubble level gauge (26) is observed, whether bubbles of the bubbles are horizontal or not is judged, and if the bubbles are not horizontal, the sliding sleeves (22) on the;
c: in the process of moving the adjusting frame (2), the vertical pipe (10) is driven to move, thereby the horizontal pipe (11), the collecting pipe (8), the branch pipe (16) and the air outlet pipe (15) are sequentially driven to move, in the process of moving the vertical pipe (10) up and down, the telescopic hose (6) is also extended or shortened, when the telescopic hose needs to be sterilized, the steam generator (13) and the air pump (9) are started, external air is pumped in through the steam generator (13), then steam is generated, the steam enters the air inlet pipe (12) through the air outlet end, the steam in the air inlet pipe (12) sequentially passes through the vertical pipe (10), the telescopic hose (6), the horizontal pipe (11), the collecting pipe (8), the branch pipe (16) and the air outlet pipe (15) and is sprayed into the placing box (14) through the air pump (9), thereby the bacteria culture disc is sterilized and sterilized, and the pollution is avoided when cells are, the detection value of the temperature sensor (28) is set in advance, the temperature in the moving frame (20) is detected by the temperature sensor (28), and when the temperature is higher than the detection temperature, the steam generator (13) and the air pump (9) stop working and are cooled.
CN201911293034.3A 2019-12-16 2019-12-16 Adjustable cell culture tray placing frame and adjusting method Withdrawn CN110903975A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111657059A (en) * 2020-06-01 2020-09-15 刘兆怀 Multifunctional culture dish placing frame for fungus planting and using method thereof
CN112430544A (en) * 2020-12-18 2021-03-02 河南茵特赛尔生物技术有限公司 Cell culture system
CN112608840A (en) * 2020-12-16 2021-04-06 李娄 Novel adjustable cell culture dish frame
CN116769595A (en) * 2023-06-19 2023-09-19 中国人民解放军空军军医大学 Low-pressure low-oxygen cell simulator

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111657059A (en) * 2020-06-01 2020-09-15 刘兆怀 Multifunctional culture dish placing frame for fungus planting and using method thereof
CN112608840A (en) * 2020-12-16 2021-04-06 李娄 Novel adjustable cell culture dish frame
CN112608840B (en) * 2020-12-16 2023-08-25 上海倍锦生物科技有限公司 Novel adjustable cell culture dish frame
CN112430544A (en) * 2020-12-18 2021-03-02 河南茵特赛尔生物技术有限公司 Cell culture system
CN116769595A (en) * 2023-06-19 2023-09-19 中国人民解放军空军军医大学 Low-pressure low-oxygen cell simulator

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