CN110878252B

CN110878252B - Culture cylinder, culture system and culture method for microorganism culture

Info

Publication number: CN110878252B
Application number: CN201911179553.7A
Authority: CN
Inventors: 肖英平; 吕文涛; 杨华; 徐娥; 王珍; 刘秀婷
Original assignee: Zhejiang Academy of Agricultural Sciences
Current assignee: Zhejiang Academy of Agricultural Sciences
Priority date: 2019-11-27
Filing date: 2019-11-27
Publication date: 2023-09-01
Anticipated expiration: 2039-11-27
Also published as: CN110878252A

Abstract

The application relates to the technical field of microorganism culture, in particular to a culture cylinder, a culture system and a culture method for microorganism culture, wherein the culture cylinder comprises a cylinder body with an open upper end and a cover body matched with the open upper end of the cylinder body, a culture medium cavity and a gas cavity are formed in the inner space of the cylinder body from bottom to top, the culture medium cavity is used for containing culture medium suitable for microorganism growth, the gas cavity is used for containing suitable gas suitable for microorganism growth, and a gas replacement device is further arranged in the cylinder body and is used for replacing the gas in the gas cavity with new suitable gas. The culture cylinder reduces the risk of death or variation or pollution in the growth and development processes of microorganisms, ensures good development speed of the microorganisms and saves culture time.

Description

Culture cylinder, culture system and culture method for microorganism culture

Technical Field

The application relates to the technical field of microorganism culture, in particular to a culture cylinder, a culture system and a culture method for microorganism culture.

Background

Microorganisms are a large group of organisms including bacteria, viruses, fungi, and some small protozoa, and are widely found in the biological kingdom, and are closely related to human life although the individual microorganisms are small. Is widely applied to various fields such as medicine and health, industry and agriculture, environmental protection and the like.

For many years, microbiologists develop scientific experiments from various aspects such as classification, physiology, genetics and the like, study the physiological and biochemical characteristics of microorganisms, and summarize a whole set of microorganism detection, screening and cultivation methods, so that microorganisms can be cultivated according to the needs of people to serve the needs of people.

At present, the following steps are generally adopted for microbial cultivation:

firstly, collecting a microbial specimen, then preprocessing an collected microbial specimen material, reducing the impurity content in the specimen material, then carrying out enrichment culture to obtain a large number of microorganisms, then selecting the microorganisms to obtain a primary selected strain, carrying out performance detection on the primary selected strain, judging whether each performance of the selected strain can meet the inoculation culture requirement, and if the strain meets the inoculation culture requirement, obtaining a qualified strain. After the qualified strain is obtained, the strain is preserved, so that the life activities of microorganisms are in a semi-permanent dormant state, and when the strain is needed to be used, the corresponding strain is resuscitated for use.

Although the above-described methods have been widely used in the field of microorganism application, the inventors have found that there are still drawbacks in the present methods, specifically as follows:

although the preservation of microorganisms can realize long-time preservation and ensure the convenience of use, the method still has the defects: on one hand, the strain preservation needs to consume a great deal of cost, and in the current microorganism application field, the strain preservation cost occupies a great cost proportion; on the other hand, during the strain preservation process, the preservation temperature, the sealing quality and the gas dryness affect the preservation quality, and when the conditions are changed, the strain is possibly not dead, mutated or polluted; on the other hand, the strain recovery also requires a great cost, and the strain death and pollution risks exist in the strain recovery process.

Therefore, there is a need to design a microbial culture system that avoids the high cost of the strain due to the preservation and recovery process, and that presents a risk of strain death and contamination.

Disclosure of Invention

The invention aims at: aiming at the defects that in the existing microorganism culture, the cost is high due to the preservation and recovery procedures of the strain and the risk of strain death and pollution exist, the microorganism culture system capable of avoiding the problem that the cost of the strain is high due to the preservation and recovery procedures and the risk of strain death and pollution exists is provided.

In order to achieve the above object, the present application provides the following technical solutions:

the utility model provides a cultivate section of thick bamboo for microorganism cultivation, includes the lower extreme closure upper end open barrel and with barrel upper end open end matched with lid the barrel inner space divide into cultivation matrix chamber and gas chamber from bottom to top, cultivation matrix chamber is used for holding the culture medium of suitable microorganism growth, gas chamber is used for holding the suitable gas of suitable microorganism growth still be provided with gas replacement device in the barrel, gas replacement device is used for will make gas in the gas chamber is replaced to new suitable gas.

In the scheme of the application, the culture medium suitable for culturing microorganisms is a conventional culture medium at present, can be a liquid culture medium or a solid culture medium or a colloid culture medium, and has different formulas and forms for different types of microorganisms, and also has different formulas and forms for gas suitable for propagation and growth of the microorganisms for different types of microorganisms, and the formulas and forms of the culture medium and the suitable gas for the microorganisms are conventional technology in the field, which is known by a person skilled in the art, so that the suitable culture medium and the gas suitable for the microorganisms can be selected or manufactured for different types of microorganisms, and are not repeated in the present specification.

In the current culture of microorganisms, the periodic gas components are critical factors influencing the propagation, growth and development of microorganisms, when the surrounding gas components are greatly changed, the propagation, growth and development of microorganisms are influenced, even the microorganisms die or are mutated or pollution is caused, and the like, which is also the critical aim of the current strain preservation, in actual work, the inventor finds that the microorganisms in the growth and propagation process, whether aerobic microorganisms or anaerobic microorganisms, mostly influence the surrounding air, the growth and development of certain microorganisms absorb part of the components in the surrounding gas, and certain microorganisms release certain gases while certain gases are exhausted, so that the growth of microorganisms is progressed along with the time, and the risks of death, mutation and pollution caused by the change of the gas components exist in the growth and development process of the microorganisms. Based on the above, in the scheme of the application, the microorganism is cultivated in the culture medium in the cylinder, the gas cavity contains proper gas, and when the gas in the gas cavity is not suitable for the growth and propagation of the microorganism any more over time, the gas in the gas cavity is replaced by new proper gas through the gas replacement device in the cylinder, so that the risk of death or variation or pollution in the growth and propagation process of the microorganism is reduced, the good development speed of the microorganism is ensured, and the cultivation time is saved.

The application also discloses a gas replacement device for the culture cylinder, which comprises a replacement component and a gas discharge component, wherein the gas discharge component is used for communicating the gas cavity with the external space of the cylinder, and the replacement component is used for pushing gas in the gas cavity so that the gas in the gas cavity is discharged out of the cylinder through the gas discharge component.

As a preferable technical scheme, the gas discharge assembly includes a communicating pipe, one end of the communicating pipe is communicated with the gas cavity, and the other end of the communicating pipe passes through the cylinder body to be communicated with an external space of the cylinder body.

As a preferable technical scheme, a stop valve is also arranged on the communicating pipe. And controlling the communication and blocking state of the communication pipe by opening and closing the shut-off valve.

As another preferable technical scheme, the communicating pipe is further provided with a unidirectional conduction device, so that the communicating pipe is unidirectional conducted in a direction from the inside of the cylinder to the outside of the cylinder.

As a preferable technical scheme, the unidirectional conduction device is arranged at one end of the communicating pipe, which is close to the gas cavity.

As an optimal technical scheme, the one-way conduction device is a one-way valve.

As a preferable technical scheme, the communicating pipe is vertically arranged along the inner wall of the gas cavity, the lower end of the communicating pipe is in clearance fit with the upper edge of the culture substrate cavity, and the upper end of the communicating pipe penetrates through the upper end part of the cylinder body to form an exhaust nozzle.

As another preferable technical scheme, the communicating pipe comprises a penetrating section and a vertical section, wherein the vertical section is vertically arranged along the outer wall of the cylinder body, one end of the penetrating section is positioned in the cylinder body and is in clearance fit with the upper edge of the culture substrate cavity, and the other end of the penetrating section penetrates out of the cylinder body and is connected with the lower end of the vertical section.

As a further preferred embodiment, the run-out section is inclined downward in a direction from inside to outside the cylinder. The penetrating section is inclined downwards, so that when the exhaust gas is condensed into liquid drop particles in the communicating pipe, the particles can be prevented from falling into the culture medium cavity, and good culture quality of microorganisms is ensured.

As a further preferable technical scheme, a downward bent U-shaped bent pipe is connected between the penetrating section and the vertical section.

As a further preferable technical scheme, a drain valve is further arranged at the bottom of the U-shaped bent pipe. When needed, the blow-down valve can be opened to discharge liquid and/or solid particles in the U-shaped bent pipe.

As a preferable technical scheme, the replacement assembly comprises a push plate and a push rod arranged on the upper side of the push plate, wherein the push rod is vertically arranged and penetrates through the cover body, the push rod is in sliding sealing fit with the cover body, and the push plate is in sliding sealing fit with the side wall of the gas cavity.

As an optimal technical scheme, when the communicating pipe is positioned in the cylinder body, a notch is arranged on the push plate corresponding to the communicating pipe, and the notch of the push plate is in sliding sealing fit with the communicating pipe.

As the preferable technical scheme, a through hole penetrating through the push rod from top to bottom is formed in the push rod, the through hole penetrates through the push plate, and a plugging cover for sealing the through hole is detachably arranged at the upper end of the push rod.

As a preferable technical scheme, the lower side surface of the push plate is in a conical shape with the middle part protruding upwards, and the through hole of the push rod is positioned at the top of the conical surface at the lower side of the push plate. The lower side of the push plate is set to be in a cone shape with the middle part protruding upwards, when the liquid culture medium enters the through hole and flows downwards to the lower side of the push plate, the liquid culture medium can flow downwards along the cone shape in a radial manner, and further the uniformity of the dispersion of the culture medium liquid is improved.

As a further preferable technical scheme, a plurality of diversion trenches are arranged on the lower side of the push plate along the direction of the bus, and the diversion trenches are uniformly distributed on the circumference. Through the setting of guiding gutter, further improvement culture medium liquid in the dispersion homogeneity of circumferencial direction.

As a further preferable technical scheme, a spray head connected with the through hole is further arranged on the lower side of the push plate corresponding to the through hole, and the spray head is used for spraying the liquid culture medium into mist. When the culture medium is liquid, the spray head is arranged to spray the supplemented culture medium liquid into mist, and the mist liquid particles gradually settle in the culture medium cavity to ensure the uniformity of dispersion, thereby further ensuring the culture quality of microorganisms.

As the preferable technical scheme, the side wall of the cylinder is also provided with an observation window, the observation window is arranged on the side wall of the cylinder corresponding to the gas cavity, the edge of the observation window is in sealing fit with the edge of the cylinder, and the observation window is made of transparent materials. The upper opening of the cylinder body is provided with the observation window, so that the microorganism condition and each component condition in the cylinder body can be observed intuitively, and the operation of operators is facilitated.

As an optimal technical scheme, the cylinder body is also provided with a barometer for monitoring the air pressure in the air cavity. Through the setting of barometer, the control to the internal air pressure of gas chamber is convenient, simultaneously, also convenient to the control to the air filling volume.

The application also discloses a microorganism culture system, which comprises the microorganism culture cylinder and also comprises a temperature control cabin, wherein a temperature control cavity capable of keeping the temperature in the cavity stable is arranged in the temperature control cabin, and all or the lower part of the microorganism culture cylinder is arranged in the temperature control cavity. In the cultivation of microorganisms, the temperature of the medium and the environment surrounding the microorganisms is also one of the important factors, whether the temperature is proper or not, determines the growth and development quality of the microorganisms, and in the actual cultivation of microorganisms, some microorganisms absorb heat, so that the temperature of a culture substrate part is reduced, some microorganisms emit heat, so that the temperature of the culture substrate part is increased, and the further growth and development of the microorganisms are often inhibited due to the change of the temperature, and even variation and death can occur in severe cases.

Therefore, in the scheme of the application, the temperature in the temperature control cavity can be controlled and regulated by arranging the temperature control cabin, and the temperature in the cavity can be kept stable, so that the temperature in the culture cylinder, particularly the temperature of the culture medium cavity, is at a temperature suitable for the growth, development and propagation of microorganisms, and further the good cultivation quality of the microorganisms is ensured.

As the preferable technical scheme, the temperature control cabin is provided with a closed cavity, a heat transfer object is filled in the closed cavity, the temperature control cabin is also provided with a temperature adjusting device for heating and/or refrigerating the heat transfer object, the upper side plate of the temperature control cabin is also provided with a concave cavity which is concave towards the cabin body, the culture cylinder is arranged in the concave cavity, and the side wall of the concave is matched with the outer wall of the culture cylinder. In this way, the temperature adjusting device is set to be at a proper temperature, and the heat transfer material is arranged in the closed cavity, wherein the heat transfer material can be water, oil or the like which can uniformly heat the concave cavity, and can also be air, so that the temperature in the concave cavity is controlled, and the temperature in the microorganism culture cylinder is controlled.

As a preferable technical scheme, the upper edge of the concave cavity is also provided with a flexible sealing gasket, and the sealing gasket is used for sealing a gap between the outer wall of the culture cylinder and the concave cavity. The arrangement of the sealing gasket ensures good heat transfer between the concave cavity and the cylinder on one hand, and improves the stability of the cylinder on the other hand.

As a preferable technical scheme, the sealing gasket is a rubber sealing gasket.

As an optimal technical scheme, the temperature control cabin is provided with a plurality of concave cavities, and each concave cavity is internally provided with one culture cylinder. Can greatly improve the culture efficiency, can greatly reduce the number of microorganisms in a single cylinder body when culturing the same number of microorganisms, can greatly reduce the risk of cross infection among the microorganisms while improving the culture quality, and also utilizes the control of mutation of the microorganisms.

As a preferable technical scheme, adjacent concave cavities are separated by a distance, so that after the culture cylinders are arranged in the concave cavities, passages for people to pass through are formed between the culture cylinders. The staff can conveniently monitor and operate each culture cylinder.

As a preferable technical scheme, the microorganism culture system further comprises a box body, wherein the temperature control cabin and the culture cylinder are arranged in the box body, and the temperature control cabin is detachably connected with the box body. Through setting up the box, improve culture system's wholeness, when reducing the contaminated of culture section of thick bamboo, still convenient transportation.

As an optimal technical scheme, the box body is a container.

As an optimal technical scheme, a gas chamber is further arranged in the box body and used for storing gas suitable for the cultured microorganisms, and an air-entrapping pipeline suitable for the push rod through hole of the culture cylinder is further arranged on the gas chamber. The gas chamber has a closed cavity therein in which fresh unused gas is stored. The air-filling pipe is provided with an air-filling nozzle matched with the pushing through hole, and the air-filling nozzle is in separable sealing fit with the pushing rod. After the air in the air cavity of the culture cylinder is exhausted, the air-entraining pipeline is matched with the through hole of the push rod, and new proper air suitable for the growth and development of microorganisms is supplemented into the air cavity.

As a preferable technical scheme, the gas chamber is arranged at the top of the box body.

As the preferable technical scheme, the gas chamber comprises a lower side plate, the edge of the lower side plate is in sliding sealing fit with the side wall of the box body, a gas storage space of the gas chamber is formed between the lower side plate and the top plate of the box body, the gas adding pipe is arranged on the lower side plate and communicated with the gas storage space of the gas chamber, and a driving device for driving the lower side plate to move vertically is further arranged in the box body. In the application, the driving device is a driving motor, and the lower side plate is driven to move upwards by the driving motor, so that the gas in the gas chamber is pressed into the gas chamber of the culture cylinder, and the filling of the proper gas is realized.

As an optimal technical scheme, an exhaust chamber is further arranged in the box body, and the exhaust chamber is communicated with the communicating pipes of the culture cylinders. In response to certain microbial cultures, the appropriate gases in the gas chamber may cause serious contamination or be toxic or harmful, and in response to such toxic or harmful gases, in the scheme of the application, the cartridge exhaust chamber is collected and then subjected to a centralized harmless treatment.

The application also discloses a microorganism culture method adopting the microorganism culture system, after the microorganism strain is obtained, the strain is cultured in the microorganism culture system, then the microorganism culture system cultured with the microorganism strain is transported to a use site, and then the microorganism in the culture system is taken out for use on site.

Compared with the traditional mode, the microbial culture method of the application adopts the microbial culture system, so that the traditional mode, namely the microbial strain preservation step and the recovery step, is directly avoided, and more importantly, the risk of death and mutation of microorganisms in the traditional preservation and recovery procedures is avoided.

As a preferable technical scheme, after the microbial strains are obtained, the gas replacement and culture medium feeding time node experiment of the microbial culture system is carried out before the strains are cultured in the microbial culture system,

the microorganism culture system gas replacement and culture medium feeding time node experiment: setting a temperature control cabin to be suitable for the growth, development and propagation of microorganisms, placing a culture medium and a strain into a culture medium cavity of a culture cylinder according to the filling amount of the culture medium and the placing amount of the microbial strain in the culture cylinder in the actual transportation process, filling proper gas into the gas cavity of the culture cylinder, starting timing, recording a time reading h0, selecting a microbial mutation rate threshold and a microbial death rate threshold according to the requirements of the actual microbial variety and the application field, and then performing the following steps:

step 1: monitoring microorganisms in the culture medium, recording time reading h1 when either the mutation rate or the death rate of the microorganisms in the culture cylinder reaches 90-95% of a threshold value,

step 2: detecting the components and the proportion of each component of the culture medium during h1, comparing the proportion of each component with the proportion of the original component of the culture medium, calculating the component d1 to be supplemented,

Step 3: the supplementing component d1 is supplemented into the culture medium cavity, then the gas in the gas cavity is replaced by new proper gas,

step 4: continuously monitoring the mutation rate and the death rate of the microorganisms, recording time reading h2 when any one of the mutation rate and the death rate of the microorganisms in the culture cylinder reaches 90-95% of a threshold value,

step 5: detecting the components and the proportion of each component of the culture medium during h2, comparing the proportion of each component with the proportion of the original component of the culture medium, calculating the component d2 to be supplemented,

step 6: the supplementing component d2 is supplemented into the culture medium cavity, then the gas in the gas cavity is replaced by new proper gas,

repeating the steps 3-6 to obtain data h3-hn and d3-dn, n is an integer greater than three,

when the strain is cultivated in the microorganism cultivation system, according to the time h1-hn, when the time reaches a certain hx, the corresponding component dx is supplemented into the cultivation matrix cavity of the cultivation cylinder, and meanwhile, the gas in the gas cavity of the cultivation cylinder is replaced by new suitable gas, wherein x is more than or equal to 1 and less than or equal to n, and x is an integer.

In the above mode of the application, the microorganism monitoring is carried out by adopting the conventional microorganism monitoring mode at present, the specific monitoring process can be definitely obtained by a person skilled in the art by adopting conventional experimental equipment and conventional technical means, experiments are not repeated in the application, in the actual culturing process, the inventor finds that, in the microorganism environment gas, not only gas exhausted in the microorganism growing process but also gas volatilized by a culture medium, but also the absorption of the microorganism to the culture medium component is usually less, the change of the gas component is larger in the same time, and the change of the gas component caused by the microorganism is more remarkable compared with the change of the culture medium component, so in the scheme, the microorganism mutation rate threshold and the death rate threshold are selected through corresponding standards and specifications of different microorganism varieties, through each time node, the whole culturing process is ensured by carrying out gas replacement and corresponding culture medium supplementing according to the time node in a culturing system, and the microorganism culturing quality is ensured under the proper environment.

As a preferable technical scheme, before the transportation of the microorganism culture system, the transportation time is estimated, the volume of the suitable gas and the amount of the supplementing component required before the transportation to the use site are calculated according to the transportation time and the number of culture cylinders in the microorganism culture system, and the required volume of the suitable gas and the amount of the supplementing component are arranged in a box body of the microorganism culture system.

As a preferable technical scheme, the culture medium supplementing component quantities required by the culture system are grouped according to the actual data h, each group of supplementing component quantities corresponds to the culture medium supplementing component quantities required to be supplemented by the whole microorganism culture system at different time points, and then each group of component quantities is equally divided according to the number of culture cylinders and is sub-packaged into supplementing component packets corresponding to the culture cylinders.

As a preferable technical scheme, after the culture system is transported to a use site, the microorganism in the culture cylinder is communicated with the culture medium and is mixed on a use product.

Compared with the prior art, the application has the beneficial effects that:

1. according to the culture cylinder, microorganisms are cultured in the culture medium in the cylinder body, the gas cavity contains proper gas, and when the gas in the gas cavity is not suitable for the growth, propagation and development of the microorganisms any more over time, the gas in the gas cavity is replaced by new proper gas through the gas replacement device in the cylinder body, so that the risk of death or variation or pollution in the growth, development and propagation process of the microorganisms is reduced, good development speed of the microorganisms is ensured, and the culture time is saved;

2. The unidirectional conduction device is arranged, so that the unidirectional conduction device only serves for exhausting, sediment during exhausting is prevented from being newly introduced into the cylinder body by proper gas to bring the sediment into the cylinder body again to cause adverse effect on microorganisms, and the culture quality of the microorganisms is further provided;

3. by arranging the U-shaped bent pipe, the aggregation of the condensed particles in the exhaust gas is facilitated, and the turbulent flow gas formed by the communicating pipe brings the particles back into the culture medium cavity in the exhaust process is reduced;

4. the through holes are arranged on the push rod, so that after the gas in the gas cavity is exhausted, new suitable gas can be supplemented into the gas cavity through the through holes of the push rod, and as the exhaust gas and the inlet gas are respectively in different channels, the condensed liquid and/or fixed particles in the exhaust gas can be prevented from entering the culture medium cavity as much as possible, and further the culture quality of microorganisms is ensured;

on the other hand, the component quality of the culture medium is also an important factor influencing the culture quality of microorganisms, and in the process of microorganism growth and development and propagation, on one hand, the absorption of microorganisms causes that part of components in the culture medium liquid are consumed, and on the other hand, because the components of the culture medium are isolated, especially the liquid culture medium, after long-time standing, part of the components of the culture medium are settled in the lower part of the liquid, the components of the culture medium change, the growth and development of microorganisms are slow, and even dormancy or variation occurs in serious cases, in the scheme of the application, corresponding components can be supplemented into the cavity of the culture medium through the through hole of the push rod, so that the components of the culture medium are in a component state suitable for microorganism growth and development, and the culture quality of microorganisms is reliably ensured;

5. In the microorganism culture system, in the microorganism culture, one of the important factors is the temperature of a culture medium and the environment surrounding the microorganism, whether the temperature is proper or not determines the growth and development quality of the microorganism, in the actual microorganism culture, some microorganisms absorb heat to reduce the temperature of a culture substrate part, some microorganisms emit heat to raise the temperature of the culture substrate part, and the change of the temperatures often inhibits the further growth and development of the microorganism, and even the situation of mutation and death possibly occurs in serious cases; therefore, in the scheme of the application, the temperature in the temperature control cavity can be controlled and regulated by arranging the temperature control cabin, and the temperature in the cavity can be kept stable, so that the temperature in the culture cylinder, particularly the temperature of the culture medium cavity, is at a temperature suitable for the growth, development and propagation of microorganisms, and further the good cultivation quality of the microorganisms is ensured;

6. compared with the traditional mode, the microorganism culture method of the application adopts the microorganism culture system of the application in the transportation link, so that the traditional mode, namely the microorganism strain preservation step and the recovery step, is directly avoided, thus, firstly, the preservation and recovery cost is avoided, and more importantly, the risk of death and variation of microorganisms in the traditional preservation and recovery procedures is avoided;

7. The method is characterized in that a microorganism mutation rate threshold and a death rate threshold are selected according to corresponding standards and specifications of different microorganism varieties, and gas replacement and corresponding culture medium supplement are carried out in a culture system according to time nodes through each time node, so that the whole culture process is ensured, microorganisms are in a proper environment, and the microorganism culture quality is ensured.

Drawings

FIG. 1 is a schematic view of a cylinder in an embodiment;

figure 2 is a schematic diagram of the structure of the temperature control cabin matched with the cylinder and the box in the specific embodiment,

the figures indicate: 1-cylinder, 2-cover, 3-culture medium cavity, 4-gas cavity, 5-communicating pipe, 6-one way conduction device, 7-U-shaped elbow, 8-push plate, 9-push rod, 10-through hole, 11-shutoff cover, 12-guiding groove, 13-shower nozzle, 14-observation window, 15-temperature control cabin, 16-temperature control cavity, 17-sealing gasket, 18-box, 19-gas chamber, 20-exhaust chamber.

Description of the embodiments

In order to make the objects, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. It will be apparent that the described embodiments are some, but not all, embodiments of the invention.

Thus, the following detailed description of the embodiments of the invention is not intended to limit the scope of the invention, as claimed, but is merely representative of some embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

It should be noted that, under the condition of no conflict, the embodiments of the present invention and the features and technical solutions in the embodiments may be combined with each other.

It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.

In the description of the present invention, it should be noted that, the terms "upper", "lower", and the like indicate an azimuth or a positional relationship based on the azimuth or the positional relationship shown in the drawings, or an azimuth or a positional relationship conventionally put in use of the inventive product, or an azimuth or a positional relationship conventionally understood by those skilled in the art, such terms are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the apparatus or element to be referred must have a specific azimuth, be constructed and operated in a specific azimuth, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like, are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance.

Example 1, as shown in fig. 1 and 2:

the utility model provides a be used for microorganism culture section of thick bamboo, includes lower extreme closure upper end open barrel 1 and with barrel 1 upper end open end matched with lid 2 the inside space of barrel 1 divide into culture substrate chamber 3 and gas chamber 4 from bottom to top, culture substrate chamber 3 is used for holding the culture medium that is fit for microorganism growth, gas chamber 4 is used for holding the fit gas that is fit for microorganism growth still be provided with gas replacement device in barrel 1, gas replacement device is used for will make gas in the gas chamber 4 is replaced to new fit gas.

In the current culture of microorganisms, the periodic gas components are critical factors influencing the propagation, growth and development of microorganisms, when the surrounding gas components are greatly changed, the propagation, growth and development of microorganisms are influenced, even the microorganisms die or are mutated or pollution is caused, and the like, which is also the critical aim of the current strain preservation, in actual work, the inventor finds that the microorganisms in the growth and propagation process, whether aerobic microorganisms or anaerobic microorganisms, mostly influence the surrounding air, the growth and development of certain microorganisms absorb part of the components in the surrounding gas, and certain microorganisms release certain gases while certain gases are exhausted, so that the growth of microorganisms is progressed along with the time, and the risks of death, mutation and pollution caused by the change of the gas components exist in the growth and development process of the microorganisms. Based on the above, in the scheme of the application, the microorganisms are cultured in the culture medium in the cylinder 1, the gas cavity 4 contains proper gas, and when the gas in the gas cavity 4 is no longer suitable for the growth, propagation and development of the microorganisms, the gas in the gas cavity 4 is replaced by new proper gas through the gas replacement device in the cylinder 1 over time, so that the risk of death or variation or pollution in the growth, development and propagation process of the microorganisms is reduced, the good development speed of the microorganisms is ensured, and the culture time is saved.

Example 2, as shown in fig. 1 and 2:

the gas displacement device for the culture cylinder comprises a displacement assembly and a gas discharge assembly, wherein the gas discharge assembly is used for communicating the gas cavity 4 with the external space of the cylinder body 1, and the displacement assembly is used for pushing gas in the gas cavity 4 so that the gas in the gas cavity 4 is discharged out of the cylinder body 1 through the gas discharge assembly.

As a preferred embodiment, the gas discharge assembly includes a communication pipe 5, one end of the communication pipe 5 communicates with the gas chamber 4, and the other end communicates with the external space of the cylinder 1 through the cylinder 1.

As a preferred embodiment, a shutoff valve is further provided in the communication pipe 5. The communication and blocking state of the communication pipe 5 is controlled by the opening and closing of the shut-off valve. By providing the shutoff valve, the shutoff valve is normally closed, and when the gas in the gas cavity 4 needs to be replaced, the shutoff valve is opened, the gas in the gas cavity 4 is pushed by the replacement assembly, the gas in the gas cavity 4 is discharged by the communicating pipe 5, and then new suitable gas is filled into the gas cavity 4 through the communicating pipe 5, so that the replacement of the gas is realized.

As another preferred embodiment, the communicating pipe 5 is further provided with a unidirectional conduction device 6 for unidirectional conduction of the communicating pipe 5 in a direction from the inside of the cylinder 1 to the outside of the cylinder 1. So that air outside the cartridge 1 cannot enter the gas chamber 4 from the closure assembly. In practical use, the inventor finds that, since the gas in the gas chamber 4 is generally wet, after the communicating pipe 5 is exhausted for many times, liquid drop particles are very likely to be accumulated in the communicating pipe 5, and even after a longer period of time, the liquid drop particles are dried and formed into solid or dust particles in the communicating pipe 5, if new suitable gas is blown into the gas chamber 4 by adopting the communicating pipe 5, the particles are possibly returned to the gas chamber 4 and fall onto the culture medium, and abnormal substances with extremely high concentration are formed on the particles and the culture medium around the particles, so that local microorganisms are extremely likely to be mutated and dead, and the culture effect of the whole microorganisms is affected.

As a preferred embodiment, the unidirectional flux device 6 is provided on the communication pipe 5 near one end of the gas chamber 4. In this way, it is further ensured that the contaminants of the communicating tube 5 do not enter the cylinder 1, and at the same time, it is also ensured that the gas in the gas chamber 4 is discharged as much as possible, and the residual gas in the pipeline is prevented from returning into the gas chamber 4.

As a preferred embodiment, the unidirectional conduction device 6 is a unidirectional valve.

As a preferred embodiment, the communicating tube 5 is vertically arranged along the inner wall of the gas chamber 4, the lower end of the communicating tube 5 is in clearance fit with the upper edge of the culture substrate chamber 3, and the upper end of the communicating tube 5 passes through the upper end portion of the cylinder 1 to form an exhaust nozzle. The communicating pipe 5 is vertically arranged on the side wall of the gas cavity 4, the exhaust nozzle is positioned at the upper end part of the cylinder body 1, so that a larger operation space is formed at the exhaust nozzle, and a plurality of cylinder bodies 1 are separated by a larger distance in order to reserve the operation space when the position of the exhaust nozzle is lower; moreover, the communicating tube 5 is arranged on the inner wall of the gas cavity 4, so that the temperature of the communicating tube 5 is consistent with the temperature in the gas cavity 4, the possibility of condensation into liquid drops when gas enters the communicating tube 5 is reduced, and the pollution risk of discharged gas to the communicating tube 5 is further reduced.

As another preferred embodiment, the communicating pipe 5 includes a penetrating section and a vertical section, the vertical section is vertically arranged along the outer wall of the cylinder 1, one end of the penetrating section is located in the cylinder 1 and is in clearance fit with the upper edge of the culture medium cavity 3, and the other end of the penetrating section penetrates out of the cylinder 1 and is connected with the lower end of the vertical section. In this scheme, with the vertical section setting of communicating pipe 5 outside barrel 1, can effectively avoid the occupation to barrel 1 inner space, also can ensure the inside smoothness of barrel 1, reduce the gap etc. that exists in the barrel 1, reduce culture substrate and assemble in gap department and lead to the problem that high concentration culture substrate appears in this local department, and then avoided these high concentration culture substrates to fall into the adverse effect that the culture substrate of below led to.

As a further preferred embodiment, the run-out section is inclined downwardly in a direction from inside the cylinder 1 to outside the cylinder 1. When the exhaust gas is condensed into liquid droplet particles in the communicating pipe 5 as described above, the particles can be prevented from falling into the culture medium chamber 3, thereby ensuring good culture quality of microorganisms.

As a further preferred embodiment, a downwardly curved U-bend 7 is connected between the run-out section and the vertical section. By arranging the U-shaped bent pipe 7, aggregation of condensed particles in the exhaust gas is facilitated, and turbulent flow gas formed by the communicating pipe 5 brings the particles back into the culture medium cavity 3 in the exhaust process is reduced.

As a further preferred embodiment, a drain valve is also arranged at the bottom of the U-shaped elbow 7. When needed, the blow-down valve can be opened to discharge liquid and/or solid particles in the U-shaped bent pipe 7.

As a preferred embodiment, the replacement assembly comprises a push plate and a push rod 9 arranged on the upper side of the push plate, wherein the push rod 9 is vertically arranged and penetrates through the cover body 2, the push rod 9 is in sliding sealing fit with the cover body 2, and the push plate is in sliding sealing fit with the side wall of the gas cavity 4. In this scheme, push rod 9 can be manpower drive also can set up to motor drive, through setting up push pedal and push rod 9, in initial position, the push pedal is located gas chamber 4 top, when needing to carry out the gas discharge, promotes the push pedal through push rod 9, makes the push pedal move down, pushes into the gaseous subassembly of discharging of gas with the gas in the gas chamber 4, and then realizes the discharge of gas, and in this kind of mode, new gas can follow communicating pipe 5 and mend gas chamber 4.

As a preferred embodiment, when the communicating pipe 5 is located in the cylinder 1, a notch is provided on the push plate corresponding to the communicating pipe 5, and the notch of the push plate is in sliding sealing fit with the communicating pipe 5. The notch matched with the communicating pipe 5 is arranged on the push plate, the part of the communicating pipe 5 positioned in the cylinder body 1 is also made to be a guide structure, the rotation of the push plate in the circumferential direction is avoided, the abrasion received by the edge of the push plate is reduced, and the sealing reliability of the push plate and the side wall of the gas cavity 4 is ensured.

As a preferred embodiment, a through hole 10 penetrating the push rod 9 from top to bottom is arranged in the push rod 9, the through hole 10 penetrates the push plate, and a plugging cover 11 for closing the through hole 10 is detachably arranged at the upper end of the push rod 9. The through hole 10 is arranged on the push rod 9 in such a way that after the gas in the gas cavity 4 is exhausted, new proper gas can be supplemented into the gas cavity 4 through the through hole 10 of the push rod 9, and as mentioned above, different channels are respectively adopted for the exhausted gas and the inlet gas, so that condensed liquid and/or fixed particles in the exhausted gas can be prevented from entering the culture medium cavity 3 as much as possible, and further the culture quality of microorganisms can be ensured;

on the other hand, the component quality of the culture medium is also an important factor influencing the culture quality of microorganisms, and during the growth, development and propagation of microorganisms, on the one hand, the absorption of microorganisms is realized, so that part of components in the culture medium liquid are consumed, and on the other hand, because the components of the culture medium are isolated, especially the liquid culture medium, after long-time standing, part of the components are settled in the lower part of the liquid, the components of the culture medium liquid change, so that the growth and the development of microorganisms are slow, and even dormancy or variation occurs in serious cases.

As a preferred embodiment, the underside of the push plate is in a shape of a cone with an upward convex middle part, and the through hole 10 of the push rod 9 is positioned at the top of the conical surface of the underside of the push plate. The lower side of the push plate is set to be in a cone shape with the middle part protruding upwards, when the liquid culture medium enters the through hole 10 and flows downwards to the lower side of the push plate, the liquid culture medium can flow downwards along the cone surface in a radial manner, and therefore the uniformity of the dispersion of the culture medium liquid is improved.

As a further preferable embodiment, a plurality of diversion trenches 12 along the bus direction are arranged at the lower side of the push plate, and the diversion trenches 12 are uniformly distributed on the circumference. By the arrangement of the diversion trenches 12, the dispersion uniformity of the culture medium liquid in the circumferential direction is further improved.

As a further preferred embodiment, a spray head 13 connected to the through hole 10 is further provided at the lower side of the push plate corresponding to the through hole 10, and the spray head 13 is used for spraying the liquid culture medium into a mist. When the culture medium is liquid, the spray head 13 is arranged to spray the culture medium liquid which is supplemented and entered into the spray, and the spray liquid particles gradually settle in the culture medium cavity 3, so that the uniformity of dispersion is ensured, and the culture quality of microorganisms is further ensured.

As a preferred embodiment, an observation window 14 is further provided on the side wall of the cylinder 1, the observation window 14 is provided on the side wall of the cylinder 1 corresponding to the gas cavity 4, the edge of the observation window 14 is in sealing fit with the edge of the cylinder 1, and the observation window 14 is made of a transparent material. The upper opening of the cylinder body 1 is provided with an observation window, so that the microorganism condition and each component condition in the cylinder body 1 can be observed intuitively, and the operation of operators is facilitated.

As a preferred embodiment, a barometer for monitoring the air pressure in the air chamber 4 is further provided on the cylinder 1. Through the setting of barometer, the control to the internal atmospheric pressure of gas chamber 4 is convenient, simultaneously, also convenient to the control to the air supply volume.

Example 3, as shown in fig. 1 and 2:

a microorganism culture system comprises the microorganism culture cylinder and further comprises a temperature control cabin 15, wherein a temperature control cavity 16 capable of keeping the temperature in the cavity stable is arranged in the temperature control cabin 15, and all or the lower part of the culture cylinder is arranged in the temperature control cavity 16. In the cultivation of microorganisms, the temperature of the medium and the environment surrounding the microorganisms is also one of the important factors, whether the temperature is proper or not, determines the growth and development quality of the microorganisms, and in the actual cultivation of microorganisms, some microorganisms absorb heat, so that the temperature of a culture substrate part is reduced, some microorganisms emit heat, so that the temperature of the culture substrate part is increased, and the further growth and development of the microorganisms are often inhibited due to the change of the temperature, and even variation and death can occur in severe cases.

Therefore, in the scheme of the application, the temperature in the temperature control chamber 16 can be controlled and regulated by arranging the temperature control chamber 15, and the temperature in the chamber can be kept stable, so that the temperature in the culture cylinder, particularly the temperature of the culture medium chamber 3, is at a temperature suitable for the growth, development and propagation of microorganisms, and further the good cultivation quality of the microorganisms is ensured.

As a preferred embodiment, the temperature control cabin 15 is a closed cavity with a closed heat transfer object filled therein, the temperature control cabin 15 is also provided with a temperature adjusting device for heating and/or cooling the heat transfer object, the upper side plate of the temperature control cabin 15 is also provided with a concave cavity recessed into the cabin body, the culture cylinder is arranged in the concave cavity, and the side wall of the concave cavity is matched with the outer wall of the culture cylinder. The concave cavity is the temperature control cavity 16, in this way, by setting the temperature regulating device to a proper temperature, by setting a heat transfer object in the closed cavity, the heat transfer object can be water, oil or other substances which can uniformly heat the concave cavity, and also can be air, so that the temperature in the concave cavity is controlled, and further the temperature in the microorganism culture cylinder is controlled.

As a preferred embodiment, a flexible sealing pad 17 is further arranged at the upper edge of the concave cavity, and the sealing pad 17 is used for sealing a gap between the outer wall of the culture cylinder and the concave cavity. The provision of the gasket 17 ensures, on the one hand, good heat transfer between the recess and the cylinder 1 and, on the other hand, also improves the stability of the cylinder 1.

As a preferred embodiment, the gasket 17 is a rubber gasket 17.

As a preferred embodiment, the temperature control cabin 15 is provided with a plurality of concave cavities, and each concave cavity is internally provided with one culture cylinder. The culture efficiency can be greatly improved, the number of microorganisms in a single cylinder body 1 can be greatly reduced when the same number of microorganisms are cultured, the culture quality is improved, the risk of cross infection among the microorganisms is also greatly reduced, and the control of mutation of the microorganisms is also utilized.

In a preferred embodiment, adjacent concave cavities are separated by a distance, so that after the culture cylinders are arranged in the concave cavities, passages for people to pass through are formed between the culture cylinders. The staff can conveniently monitor and operate each culture cylinder.

As a preferred embodiment, the microorganism culture system further comprises a box 18, the temperature control cabin 15 and the culture cylinder are arranged in the box 18, and the temperature control cabin 15 and the box 18 are detachably connected. By providing the box 18, the integrity of the culture system is improved, the pollution to the culture cylinder is reduced, and the transportation is convenient.

In a preferred embodiment, the container 18 is a container.

As a preferred embodiment, a gas chamber 19 is further provided in the case 18, the gas chamber 19 is used for storing gas suitable for the microorganism to be cultured, and an air-adding pipeline suitable for the through hole 10 of the push rod 9 of the culture cylinder is further provided on the gas chamber 19. The gas chamber 19 has a closed cavity therein in which fresh unused gas is stored. The air filling pipe is provided with an air filling nozzle which is matched with the pushing through hole 10, and the air filling nozzle is in separable sealing fit with the push rod 9. After the air in the gas cavity 4 in the culture cylinder is discharged, the gas cavity 4 is supplemented with new proper gas suitable for the growth and development of microorganisms through the cooperation of the gas filling pipeline and the through hole 10 of the push rod 9.

As a preferred embodiment, the gas chamber 19 is provided at the top of the tank 18.

As a preferred embodiment, the gas chamber 19 includes a lower side plate, a sliding seal fit is provided between an edge of the lower side plate and a side wall of the box 18, a gas storage space of the gas chamber 19 is formed between the lower side plate and a top plate of the box 18, the gas adding pipe is disposed on the lower side plate and is communicated with the gas storage space of the gas chamber 19, and a driving device for driving the lower side plate to move vertically is further disposed in the box 18. In the application, the driving device is a driving motor, and the lower side plate is driven to move upwards by the driving motor, so that the gas in the gas chamber 19 is pressed into the gas cavity 4 of the culture cylinder, and the filling of the proper gas is realized.

In a preferred embodiment, an exhaust gas chamber 20 is further provided in the case 18, and the exhaust gas chamber 20 communicates with the communication pipe 5 of each of the culture cylinders. Suitable gases in the gas chamber 4 may cause serious pollution or be toxic or harmful in response to certain microorganism cultures, and in response to such toxic or harmful gases, the cartridge waste gas chamber 20 is collected and then subjected to a concentration of harmless treatment in the scheme of the present application.

Example 4, as shown in fig. 1 and 2:

after the microbial strain is obtained, the strain is cultivated in the microbial culture system, the microbial culture system cultivated with the microbial strain is transported to a use site, and then the microorganisms in the culture system are taken out for use on site.

As a preferred embodiment, after the microbial species is obtained, the microbial culture system gas displacement and culture substrate feeding time node experiment is performed before the species is cultured in the microbial culture system,

The gas replacement and culture medium feeding time node experiment of the microorganism culture system comprises the following steps: setting a temperature control cabin 15 to be suitable for the growth, development and propagation of microorganisms, placing a culture medium and a strain into a culture medium cavity 3 of a culture cylinder according to the filling amount of the culture medium and the strain placing amount of the microorganisms in the culture cylinder in the actual transportation process, filling proper gas into a gas cavity 4 of the culture cylinder, starting timing, recording a time reading h0, selecting a microorganism mutation rate threshold and a microorganism death rate threshold according to the requirements of the actual microorganism variety and the application field, and then performing the following steps:

step 3: the supplementing component d1 is supplemented into the culture medium cavity 3, then the gas in the gas cavity 4 is replaced by new proper gas,

step 6: the supplementing component d2 is supplemented into the culture medium cavity 3, then the gas in the gas cavity 4 is replaced by new proper gas,

when the strain is cultivated in the microorganism cultivation system, according to the time h1-hn, when the time reaches a certain hx, the corresponding component dx is supplemented into the culture medium cavity 3 of the cultivation cylinder, and meanwhile, the gas in the gas cavity 4 of the cultivation cylinder is replaced by new suitable gas, wherein x is more than or equal to 1 and less than or equal to n, and x is an integer.

As a preferred embodiment, the transport time is estimated before the transport of the microorganism culture system, the amount of the appropriate gas and the amount of the replenishing component required before the transport to the site of use are estimated based on the transport time and the number of culture cylinders in the microorganism culture system, and the desired amount of the appropriate gas and the amount of the replenishing component are arranged in the case 18 of the microorganism culture system. In this scheme, the desired suitable gas volume and culture medium composition are estimated by estimating the incubation time of the microorganism in the microorganism incubation system and then combining the incubation time with the experimental data, and are not further described in the present application since they can be unambiguously derived by a person skilled in the art through conventional knowledge.

As a preferred embodiment, the culture medium supplementing component amounts required by the culture system are grouped according to the actual data h, each group of supplementing component amounts corresponds to the culture medium supplementing component amounts required to be supplemented by the whole microorganism culture system at different time points, and then each group of component amounts is equally divided according to the number of the culture cylinders and is sub-packaged into supplementing component sub-packages corresponding to the culture cylinders. In actual use, corresponding groups are selected at different time nodes, and then culture medium supplementing components of a certain group of components are supplemented into the culture medium cavity 3 of the culture cylinder, so that the operation process is convenient, the quantification is good, and the accuracy of supplementing the components is ensured.

As a preferred embodiment, the microorganism-communicating culture medium in the culture vessel is mixed together on the product to be used after the culture system is transported to the site of use. The method can fully utilize the culture medium, particularly can form good microbial environment buffer, and avoid excessive adverse effects on the growth, development and propagation of microorganisms due to overlarge environmental gap.

The above embodiments are only for illustrating the present invention and not for limiting the technical solutions described in the present invention, and although the present invention has been described in detail in the present specification with reference to the above embodiments, the present invention is not limited to the above specific embodiments, and thus any modifications or equivalent substitutions are made to the present invention; all technical solutions and modifications thereof that do not depart from the spirit and scope of the invention are intended to be included in the scope of the appended claims.

Claims

1. A method of culturing a microorganism, characterized by: the culture cylinder is adopted to culture the microorganism,

the culture cylinder comprises a cylinder body with an upper end being opened and a lower end being closed, and a cover body matched with the upper end opening end of the cylinder body, wherein the inner space of the cylinder body is divided into a culture medium cavity and a gas cavity from bottom to top, the culture medium cavity is used for containing culture medium suitable for microorganism growth, the gas cavity is used for containing suitable gas suitable for microorganism growth, and a gas replacement device is also arranged in the cylinder body and is used for replacing the gas in the gas cavity with new suitable gas;

the gas displacement device comprises a displacement assembly and a gas discharge assembly, wherein the gas discharge assembly is used for communicating the gas cavity with the outer space of the cylinder, and the displacement assembly is used for pushing gas in the gas cavity so that the gas in the gas cavity is discharged out of the cylinder through the gas discharge assembly;

The gas discharge assembly comprises a communicating pipe, one end of the communicating pipe is communicated with the gas cavity, and the other end of the communicating pipe penetrates through the cylinder body to be communicated with the external space of the cylinder body;

the communicating pipe is also provided with a one-way conduction device, so that the communicating pipe is conducted unidirectionally in the direction from the inside of the cylinder to the outside of the cylinder;

the one-way conduction device is arranged at one end, close to the gas cavity, of the communicating pipe;

the communicating pipe comprises a penetrating section and a vertical section, the vertical section is vertically arranged along the outer wall of the cylinder, one end of the penetrating section is positioned in the cylinder and is in clearance fit with the upper edge of the culture medium cavity, the other end of the penetrating section penetrates out of the cylinder and is connected with the lower end of the vertical section, and the penetrating section is inclined downwards in the direction from the inside of the cylinder to the outside of the cylinder;

a U-shaped bent pipe which is bent downwards is connected between the penetrating section and the vertical section;

a drain valve is further arranged at the bottom of the U-shaped bent pipe, and can be opened to discharge liquid and/or solid particles in the bent pipe when needed;

the replacement assembly comprises a push plate and a push rod arranged on the upper side of the push plate, the push rod is vertically arranged and penetrates through the cover body, sliding sealing fit is formed between the push rod and the cover body, and the push plate is in sliding sealing fit with the side wall of the gas cavity;

When the communicating pipe is positioned in the cylinder body, a notch is arranged on the push plate corresponding to the communicating pipe, and the notch of the push plate is in sliding sealing fit with the communicating pipe;

a through hole penetrating through the push rod from top to bottom is formed in the push rod, the through hole penetrates through the push plate, and a plugging cover for closing the through hole is detachably arranged at the upper end of the push rod;

the lower side of the push plate is in a conical shape with an upward convex middle part, a through hole of the push rod is positioned at the top of the conical surface at the lower side of the push plate, a plurality of diversion trenches along the bus direction are arranged at the lower side of the push plate, the circumference of each diversion trench is uniformly distributed, a spray head connected with the through hole is also arranged at the lower side of the push plate corresponding to the through hole, and the spray head is used for spraying the liquid culture medium into mist;

the microorganism culture method further comprises a temperature control cabin, wherein a temperature control cavity capable of keeping the temperature in the cavity stable is arranged in the temperature control cabin, all or the lower part of the culture cylinder is arranged in the temperature control cavity, the temperature control cabin is provided with a closed cavity filled with a heat transfer substance, the temperature control cabin is also provided with a temperature regulating device for heating and/or refrigerating the heat transfer substance, the upper side plate of the temperature control cabin is also provided with a concave cavity recessed into the cabin body, the culture cylinder is arranged in the concave cavity, the side wall of the concave cavity is matched with the outer wall of the culture cylinder, the concave cavity is the temperature control cavity,

The upper edge of the concave cavity is also provided with a flexible sealing gasket which is used for sealing the gap between the outer wall of the culture cylinder and the concave cavity,

after the microbial strain is obtained, culturing the strain in a microbial culture system, transporting the microbial culture system cultured with the microbial strain to a use site, and taking out the microorganism in the culture system for use on site;

after the microbial strains are obtained, the gas replacement and culture medium feeding time node experiment of the microbial culture system is performed before the strains are cultured in the microbial culture system.

2. The method of claim 1, wherein: and a stop valve is also arranged on the communicating pipe.

3. The method for culturing microorganisms according to claim 1 or 2, wherein: the communicating pipe is vertically arranged along the inner wall of the gas cavity, the lower end of the communicating pipe is in clearance fit with the upper edge of the culture substrate cavity, and the upper end of the communicating pipe penetrates through the upper end part of the cylinder body to form an exhaust nozzle.