KR101087858B1 - Water toxicity detecting apparatus and method using bio-luminescent bacteria - Google Patents

Abstract

The present invention relates to a method and apparatus for automatically monitoring aquatic toxicity using luminescent microorganisms, and more specifically, to a temperature control chamber (2) and air for adjusting an internal temperature to maintain sterilization. A medium storage tank 1 composed of an air outlet 12 for discharging; The medium storage tank (1) is connected to the medium supply pipe and continuously supplied to the continuous reaction tank, and the inside of the continuous reaction tank (3) and the microorganism constant flow into the luminescence measurement unit to constantly survive without changing the concentration of microorganisms with time. A tube 16 in the form; A J-shaped pipe 36 into which the river water taken by the water intake pump 40 flows in and quantitatively weighs the river water; A light emission measurement unit 4 connected to the two J-shaped pipes 16 and 36 to determine a toxicity by measuring a light emission state by moving a predetermined amount of light-emitting microorganisms and river water; The present invention relates to an automatic water-based toxicity monitoring system using a light-emitting microorganism consisting of a contaminated water storage tank 5 that separates and stores river water whose toxicity is determined in the luminescence measurement unit.

According to the present invention, unlike the prior art, the medium storage tank that maintains the luminescent microorganism at a constant concentration regardless of time and supplies the medium to the continuous reaction tank 3 always maintains 80 ° C. to 90 ° C. to contaminate the medium by other microorganisms. It is prevented and the continuous reaction tank (3) maintains a constant concentration without contamination for more than 30 days, so it is easy to grasp the toxicity. There is an advantage.

Luminescent Microorganism, River Water, Medium, Luminance, Continuous Reaction

Description

Water toxicity detecting apparatus and method using bio-luminescent bacteria

The present invention relates to an automatic remote monitoring method and apparatus for aquatic toxicity using luminescent microorganisms, and more specifically, to detect and automatically alarm toxic substances entering a river or a lake by using bioluminescent phenomena of luminescent microorganisms. It is closely related to water quality monitoring and management such as water, lake, groundwater, intake, water purification plant, sewage treatment plant, wastewater treatment plant and fish farm.

As the industry of modern society develops, pollution accidents occur frequently. For example, accidents such as factory wastewater, oil spills, livestock wastewater inflows, and overuse of pesticides such as phenol pollution in the Nakdong River occur. There is an urgent need to operate a system that alerts to the ingress of contaminants that can respond quickly to these incidents. There is also a need for technology to be used for water quality measures of water resources by using the early warning system of toxic substances and the results of regular monitoring in intake stations for safe tap water supply.

Many related technologies are being developed.

For example, Korean Patent Registration No. 0300445 relates to a continuous monitoring method for toxic substances in water based on light emitting microorganisms, and a continuous monitoring kit therefor, wherein the light emitting is continuously supplied and continuously supplied with light emitting microorganisms. Measuring the luminescence of the microorganism with a control and measuring the luminescence by mixing the sample collected in the luminescent microorganism whose luminescence was measured and analyzing the difference between the luminescence of the control and the luminescence of the luminescent microorganism including the sample. The continuous monitoring method of toxic substances using continuous cultured luminescent microorganisms and the cultivation tank sequentially cultivating the luminescent microorganisms with the medium supplied from the medium supply tank, the medium supply tank, and the luminescence of the culture solution supplied from the culture tank as control. Samples supplied from the control and measuring tanks to be measured and microorganisms supplied from the control and measurement tanks Consisting of a luminescence measuring tank, a microbial culture tank, a control measuring tank, a means for measuring the amount of light in the luminescence measuring tank and a means for analyzing the difference in the luminescence obtained from the control measuring tank and the luminescence measuring tank Consists of a continuous monitoring kit of toxic substances using continuous cultured luminescent microorganisms.

However, the present invention, unlike the prior art described above, sterilize the microbial medium and maintain the temperature of the medium at 80 ℃ ~ 90 ℃ to sterilize the microorganisms in the medium to prevent the introduction of microorganisms of other species and can be supplied for more than 30 days The amount is stored in the medium storage tank 1 so that the luminescent microorganisms can be used continuously without additional supply. In addition, unlike the prior art described above, it is possible to carry out the present invention with the number of the quantitative pumps 6 as one, thereby saving energy and maintaining luminescence at all times even when there is no toxic substance through aeration, and a reaction tank for a control. The toxicity is determined by comparing the degree of luminescence over time. As such, the present invention shows a remarkable difference in terms of prior art, configuration, and effect.

The present invention maintains a constant concentration of light-emitting microorganisms at all times regardless of time while improving the disadvantages of the prior art, and the continuous reaction tank (3) maintains a constant concentration without contamination for more than 30 days to facilitate the detection of toxicity quickly and accurately The present invention provides a method and apparatus for automatic remote monitoring of aquatic toxicity using luminescent microorganisms that detects and responds to toxic substances, and enables low production and maintenance costs and easy maintenance.

In order to solve the above technical problem, the present invention consists of a temperature control chamber (Temp. Control chamber) 2 for adjusting the temperature inside to maintain the sterilization state and the air outlet 12 for discharging air A medium storage tank 1; It is connected to the medium storage tank (1) and the medium supply pipe to be continuously supplied to the continuous reaction tank, and to introduce a constant amount of the continuous reaction tank (3) and microorganisms constant survival without changing the concentration of microorganisms over time inside the light emitting side government J-shaped tube 16; A J-shaped pipe 36 into which the river water taken by the water intake pump 40 is introduced and quantitatively measured to take river water; A light emission measurement unit 4 connected to the two J-shaped pipes 16 and 36 to determine a toxicity by measuring a light emission state by moving a predetermined amount of light-emitting microorganisms and river water; It can be solved by the automatic water-based toxicity monitoring system using a light-emitting microorganism consisting of a contaminated water storage (5) tank for separating and storing the river water is determined toxicity in the luminescence measurement unit.

In addition, the step of storing the medium to be supplied to the continuous reaction tank (3) through the medium storage tank (1) and continuously supplying the medium of the medium storage tank (1) from the medium supply pipe (14) to the continuous reaction tank (3) Maintaining the concentration and circulating the luminescent microorganisms with a metering pump to fill the J-shaped pipe 16 and supplying a part of the river water taken through the intake pump 4 to the J-shaped pipe 36. If the toxicity is determined after the step of filling the river water, transferring the microorganisms of the two J-shaped pipes (16, 36) and the river water to the luminescence measuring unit and the luminescence measurement according to the time in the luminescence measuring unit, the contaminated water is contaminated And storing the water in the storage tank (5), issuing an alarm after determining the toxicity and transmitting it to a person in charge through wired and wireless communication, and storing and quantitatively analyzing the raw water of the measured river water. Light microorganism can be solved by a water-based toxicity automatic remote monitoring method.

The present invention maintains the light-emitting microorganisms at a constant concentration regardless of time at all times, and always maintains 80 ℃ ~ 90 ℃ through a device for supplying a medium to the continuous reaction tank (3) to prevent contamination by other microorganisms, and the continuous reaction tank (3 ) Maintains a certain concentration without contamination for more than 30 days, so it is easy to grasp the toxic, and when measuring luminescence, it measures the luminescence by aeration of air at a constant speed and makes it easy to grasp the toxic substances. It can be maintained at low maintenance cost.

In order to solve the above problems, the present invention will be described in more detail with reference to the following drawings and examples.

The terms used in the present invention and the like are merely examples given to specifically illustrate the practice of the present invention, and thus the scope of the present invention is not limited thereto.

1 is a schematic diagram showing the configuration of the overall apparatus of the present invention. The present invention consists of a medium storage tank (1), a continuous reaction tank (3), and a luminescence measurement unit (4) as a whole.

In the present invention, the light emitting microorganism should be periodically cultivated in a laboratory and put in a monitoring device, and the light emitting microorganism is refrigerated and stored, but the light emission in the monitoring device is solved in order to solve the disadvantage that the activity of the microorganism varies over time and thus toxicity cannot be accurately measured. The reactor (3) that can be grown uniformly in a microorganism is placed to inject the microbial feed at a constant speed so that the microorganism can grow continuously and maintain a uniform concentration. The medium to be fed the microorganism is stored in the medium storage tank (1) to maintain about 80 ℃ ~ 90 ℃ to prevent decay and to store the amount of about 4L to supply nutrients that can grow microorganisms for 30 days. Make sure to maintain constant light emission at all times.

It is possible to check whether it works properly by testing heavy metals such as lead, cadmium, and arsenic in real river water.

At a constant rate, the medium is supplied to the continuous reactor 3 and aerated at a constant rate, so that the concentration of the luminescent microorganisms is kept constant and thus the emission amount is kept constant. Microorganisms of 0.5 to 3 ㏄ (adjustable by varying the size of the J-shaped tube) of the continuous reactor 3 enter the J-shaped tube 16 represented in FIG. 2 below, and are transferred to the luminescence measuring unit, and another J-type. The pipe 36 collects river water, enters the luminescence measurement unit, mixes with microorganisms, and measures luminescence at 5 minute intervals for 25 minutes. Air is always aerated at a constant rate during measurement. Conventional technology uses four emission measurement devices, but the emission measurement unit of the present invention uses a known emission measurement device, and the description thereof is omitted. Economic and spatial effects were achieved by installing and using only one luminescence measuring device.

2 is a view showing the structure of the light-emitting microbial continuous reactor of the present invention.

The medium is maintained at 80 ° C ~ 90 ° C so as not to rot from the medium storage tank (1) is supplied at a constant flow rate through the tube (14). The capacity of the continuous reactor 3 is maintained at 100-200cc and the medium is 10-18 hours in the present invention to allow the microorganisms to grow for 30 days.

A J-shaped tube 16 is placed on top of the continuous reactor 3. A J-shaped tube 16 is used to supply a certain amount of microorganisms. The tube is continuously filled with a certain amount and once discharged using an air-compressor (7) and then refilled, the microorganism is used. The microorganisms in the continuous reactor continue to circulate through the tube 16 and fall to the J-shaped tube 16 and overflow. By changing the capacity of the J-shaped tube 16, it is possible to change the amount of microorganisms supplied to 0.5 to 3 kPa. In the circulation process, air is supplied using an air-generator 8. Since the light emitting microorganisms require oxygen in order to emit light, light emission of the light emitting microorganisms is kept constant by supplying the air to aeration. In order to keep the capacity of the continuous reactor 3 constant at 100-200 cc, the diameter of the tube 11 should be larger than that of the tube 14 using the tube 11. If you want to increase the microbial capacity of the continuous reactor is possible to adjust the capacity by moving the end of the tube (14) up.

Conventional prior art does not present a ratio of river water (sample) and microbial amount, but in the present invention, the ratio of the microorganism and the river water (sample) ranges from 1: 1 to 1:10. In case of high toxicity such as factory waste water, keep 1: 1 and keep 1:10 in water containing less toxic substances.

In the present invention, the light emission measurement unit measures 25 minutes at 5 minute intervals and keeps constant for 25 minutes when only microorganisms are put. Determine the toxicity level by using the average amount of light emission of 15 minutes, 20 minutes, or 20 minutes and 25 minutes, and use the ratio of the measured mean value to the initial value. In general, the present invention can adjust the concentration and the retention time of the medium, and according to the concentration and retention time of the medium, the luminescence (RLU) is maintained at 200000 ~ 1500000.

 The medium enters at a constant flow rate. Maintain a flow rate that will allow you to operate for at least 30 days. The amount of inlet medium was set to 4 liters for 30 days of operation, but it could be increased further as needed.

In addition, the present invention uses six metering pumps in the prior art, while the present invention uses one metering pump 6 and uses a J-shaped tube for inputting a certain amount of microorganisms and samples. Inexpensive maintenance and management can be achieved.

3 is a view showing the structure of the water intake and storage tank of the river water of the present invention.

River water taken through the intake pump is moved through a separate river intake pipe. J-type pipe 36 is used to take in a certain amount of sample, and air-compressor 7 is used to transfer the sample to J-type pipe 36. Discard the filled river water (sample), refill and enter the luminescence measurement unit. In general, within the above ratio of river water, 1 to 5 cc of sample and 0.5 to 3 cc of microorganism are reacted. When toxicity is determined, valve 2 (10) is closed and valve 3 (10) is opened and stored in a separate contaminated water storage tank (5). If the toxicity is not normally determined, the valve 10 is managed in reverse to the above.

4 is a sectional view of the medium storage tank 1.

4 is configured to be suitable for feeding the luminescent microorganisms continuously for 30 days in a size of 4L as the medium storage tank (1). The circumference of the medium storage tank (1) is surrounded by a temperature control chamber (2) to maintain a temperature of about 80 ℃ ~ 90 ℃ to prevent corruption of the medium. In addition, in order to maintain a sterile state with maintaining a constant temperature, the filter 12 is installed to manage the medium storage tank (1).

5 is a system flow diagram for implementing the present invention.

The luminescent microorganism is continuously circulated in the continuous reactor (3) and filled in about 1 to 5 cc in the J-shaped tube and is transferred to the luminescence measuring unit, and the river water is collected in the same way as the sample or the same and transferred to the luminescence measuring unit. In the luminescence measurement unit, the luminescence of the mixed microorganism and the river water (sample) is measured every 5 minutes for 25 minutes. After the measurement, the emission reduction rate is calculated and the reduction amount is less than the reference value using a separate alarm device connected to the present invention to inform the person in charge and take emergency measures. If the reduction is at or above the baseline, the same measurement is carried out again every 30 minutes for river water and raw water. It is determined whether the alarm is made within 10 minutes after the above-mentioned sample water is introduced. When an alarm sounds, the person in charge takes emergency measures and keeps the number of measuring points separately to carry out measures through quantitative and qualitative analysis.

Hereinafter, the effect of the present invention through the embodiment based on the above technical configuration will be described.

[Example]

The nutrient medium of the luminescent microorganism is composed of the components shown in Table 1 below in a single dose.

TABLE 1

The composition of the badge density Peptone (g / ℓ) 10 Yeast Extract (g / ℓ) 3 Sodium chloride (g / ℓ) 30 Glycerol (ml / l) One

In the continuous reactor (3), the medium stayed for about 18 hours, and the luminescence (RLU) of the reactor was kept constant at 1200000 to 1300000 for 30 days, and the luminescent microorganism was kept constant in the absence of toxic substances as shown in FIG. Looking at the change in the luminescence according to the change in the lead concentration of the exhibits excellent effect enough to be able to take action according to the change in the luminescence within 10 minutes.

Through this, the light-emitting microorganisms of the continuous reaction tank (3) are always maintained at a constant concentration regardless of time, and through the medium storage tank for supplying the medium to the continuous reaction tank (3) is always maintained at 80 ℃ ~ 90 ℃ due to other microorganisms Contamination is prevented, and the continuous reaction tank (3) maintains a constant concentration without contamination for more than 30 days, so it is easy to identify toxicities, and microorganisms and river water are mixed so that air can be expelled at a certain speed during measurement of luminescence while toxic measurement is easy. It can be seen that the present invention capable of quickly and accurately detecting and responding to toxic substances and maintaining at low manufacturing and maintenance costs can sufficiently realize the effect to be implemented.

1 is a schematic view of the overall configuration of the present invention

2 is a configuration of a light emitting microbial continuous reactor of the present invention

3 is a river intake configuration of the present invention

Figure 4 is a block diagram of a medium storage tank of the present invention

5 is a system flow chart for implementing the present invention.

Figure 6 is a change in luminescence with time for the lead concentration change through the present invention

<Description of the symbols for the main parts of the drawings>

1: badge storage tank

2: temperature control chamber

3: continuous reactor

4: Luminescence measuring unit

5: contaminated water storage

6: metering pump

7: air compressor

8: air generator

9: air outlet

10: valve

11: water level control outlet

12: air filter

13: quantitative metering circulation piping

14 medium supply piping

15: compressed air supply pipe

16: J-shaped tube in a continuous reactor

17: river intake pipe

36: J type pipe in river intake part

40: intake pump

Claims (9)

delete delete A medium storage tank (1) comprising a temperature control chamber (2) for controlling an internal temperature to maintain a sterilization state, and an air discharge port (9) for discharging air; A continuous reaction tank (3) connected to the medium storage tank (1) and a medium supply pipe to allow the light emitting microorganism to survive; Located at the top of the continuous reactor (3) and the light-emitting microorganisms filled in the J-shaped tube 16 of the continuous reaction tank 3 and the water taken in another J-shaped tube 36 by the intake pump 40 is mixed A luminescence measurement unit 4 for determining the toxicity by measuring the luminescence state; Contaminated water storage tank (5) for separating and storing the river water is determined toxicity in the luminescence measurement unit (4); The continuous reaction tank (3) includes an air-generator (8) for supplying air to aeration and maintaining a constant concentration of luminescent microorganisms; An air discharge port 9 for discharging air supplied through the air-generator 8 and air; An air filter (12) installed at the air discharge port (9) to purify the air discharge; A valve 10 capable of controlling the air; Water-based toxicity automatic remote monitoring device using a light-emitting microorganism, characterized in that consisting of an outlet for controlling the water level of the continuous reaction tank (3). The method of claim 3, The continuous reaction tank (3) is a quantitative pump (6) for continuously circulating the light-emitting microorganisms through the circulation pipe to drop into the J-shaped pipe (16); An automatic water-based toxicity monitoring system using a light-emitting microorganism, characterized in that an air-compressor (7) used to empty the circulating light-emitting microorganisms is added. The method according to any one of claims 3 to 4, The medium storage tank (1) is maintained at 80 ℃ ~ 90 ℃ by a temperature control chamber (Temp. Control chamber) (2) in order to maintain the sterilization state and the luminescent microorganisms in the continuous reaction tank (3) for 30 days survival For 4L-8L of medium; The continuous reaction tank (3) stores the light emitting microorganism of 100 ~ 200cc and maintain the luminescence (RLU) 200000 ~ 1500000 and circulated through the circulation pipe 13 of 0.5cc ~ 3cc of the J-shaped pipe (16) Supplying luminescent microorganisms; In the luminescence measurement unit (4) by using the light-emitting microorganism to perform the function for reacting by moving the light-emitting microorganism 0.5cc ~ 3cc and 1cc ~ 5cc of the river water taken through the intake pump 40 and measuring the pollution degree of the river water Water-based toxicity automatic remote monitoring device Storing the medium to be supplied to the continuous reaction tank (3) through the medium storage tank (1); Supplying the medium of the medium storage tank (1) from the medium supply pipe (14) to grow luminescent microorganisms in the continuous reaction tank (3); Circulating the stored luminescent microorganisms in a metering pump (6) and supplying them to the J-shaped pipe (16); Supplying a part of the river water taken through the water intake pump 40 and passing through the river intake pipe 17 to the J-shaped pipe 36; Transferring the river water and the luminescent microorganisms to the luminescence measurement unit 4 to measure luminescence; Storing the collected contaminated water in a contaminated water storage tank (5) when toxicity is determined after the measurement in the luminescence measuring unit (4); Issuing an alarm after determining the toxicity and transmitting the alarm to a person in charge through wired or wireless communication; Storing and quantifying or qualitatively analyzing the raw water of the measured river water; Automatic remote monitoring method of aquatic toxicity using luminescent microorganisms. The method of claim 6, In order to maintain the sterile state of the culture medium storage tank (1) through the temperature control chamber (Temp. Control chamber) (2) it is added to maintain the water-based toxic automatic remote using a light-emitting microorganism, characterized in that it is added Monitoring method.  The method of claim 6, It is possible to supply to the continuous reaction tank (3) for 30 days with 4L ~ 8L of the medium supplied from the medium storage tank (1) and maintaining the luminescence (RLU) of the luminescent microorganisms 200000 ~ 1500000; Supplying 0.5 cc to 3 cc of the J-shaped tube 16 while circulating microorganisms in the continuous reaction tank 3 by a metering pump connected to the circulation pipe; Supplying air to the continuous reactor (3) through an air-generator (8) to aeration and maintaining a constant concentration of luminescent microorganisms; Filtering the gas and air generated during the circulation of the light-emitting microorganism and the aeration of the air-generator (8) through the air filter (12) and discharging it to the air outlet (9); When the water level of the continuous reaction tank (3) exceeds a certain level, the step of discharging through the outlet 11 for adjusting the water level, characterized in that the automatic remote monitoring method using water-based microorganisms. The method according to any one of claims 6 to 8, Transferring 1cc to 5cc of the river water taken through the water intake pump 40 to the light emitting microorganisms circulated through the J-shaped pipe 16 and supplied to the light emission measuring tank; Before the luminescent microorganisms and the river water are transported, the step of emptying the previous luminescent microorganisms remaining in the J-shaped pipe 16 and the river water through an air-compressor 7 is added. Automatic remote monitoring method using waterborne toxicity.

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