CN110877048B - Method for repairing lead-chromium composite severely-polluted soil by utilizing humic acid and sudan grass - Google Patents
Method for repairing lead-chromium composite severely-polluted soil by utilizing humic acid and sudan grass Download PDFInfo
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- CN110877048B CN110877048B CN201911235453.1A CN201911235453A CN110877048B CN 110877048 B CN110877048 B CN 110877048B CN 201911235453 A CN201911235453 A CN 201911235453A CN 110877048 B CN110877048 B CN 110877048B
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- 239000002689 soil Substances 0.000 title claims abstract description 112
- 235000015503 Sorghum bicolor subsp. drummondii Nutrition 0.000 title claims abstract description 61
- 229910052804 chromium Inorganic materials 0.000 title claims abstract description 57
- 239000011651 chromium Substances 0.000 title claims abstract description 57
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 title claims abstract description 36
- 239000004021 humic acid Substances 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims abstract description 22
- 239000002131 composite material Substances 0.000 title claims abstract description 16
- 244000064817 Sorghum halepense var. sudanense Species 0.000 title claims abstract 23
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 claims abstract description 32
- 230000012010 growth Effects 0.000 claims abstract description 23
- 239000002344 surface layer Substances 0.000 claims abstract description 18
- 230000008636 plant growth process Effects 0.000 claims abstract description 9
- 238000005259 measurement Methods 0.000 claims abstract description 3
- 238000009331 sowing Methods 0.000 claims description 16
- 241000196324 Embryophyta Species 0.000 abstract description 33
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 23
- 230000008439 repair process Effects 0.000 abstract description 13
- 230000007613 environmental effect Effects 0.000 abstract description 8
- 230000035558 fertility Effects 0.000 abstract description 5
- 231100000419 toxicity Toxicity 0.000 abstract description 3
- 230000001988 toxicity Effects 0.000 abstract description 3
- 230000004083 survival effect Effects 0.000 abstract description 2
- 244000170625 Sudangrass Species 0.000 description 38
- 238000012216 screening Methods 0.000 description 11
- 238000012360 testing method Methods 0.000 description 8
- 230000000694 effects Effects 0.000 description 6
- 230000008901 benefit Effects 0.000 description 4
- 240000006394 Sorghum bicolor Species 0.000 description 3
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 230000035784 germination Effects 0.000 description 3
- 230000008635 plant growth Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000207894 Convolvulus arvensis Species 0.000 description 2
- 241000209046 Pennisetum Species 0.000 description 2
- 238000010306 acid treatment Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000003900 soil pollution Methods 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 244000148137 Patrinia villosa Species 0.000 description 1
- 235000019109 Patrinia villosa Nutrition 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 238000010668 complexation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000002905 metal composite material Substances 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000004460 silage Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000002336 sorption--desorption measurement Methods 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
Abstract
The invention relates to a method for repairing lead-chromium composite severely-polluted soil by utilizing humic acid and sudan grass, which effectively solves the problems that the humic acid reduces the biological effectiveness of heavy metal lead-chromium, relieves the toxicity of heavy metal lead-chromium to sudan grass, creates conditions for the growth of sudan grass in medium and severely-polluted areas, improves the repairing efficiency of sudan grass to lead-chromium composite-polluted soil, uniformly spreads humic acid on the surface layer of lead-chromium-polluted soil, ensures that the chromium on the surface layer of the soil is more than or equal to 275.4mg/kg, the lead is more than or equal to 989.7mg/kg, plows the soil, rakes, divides ridges, sows sudan grass seeds, performs watering once every 3-7 days in the plant growth process, manages according to the normal growth requirement of sudan grass, acquires soil samples for measurement, ensures that the lead content of the soil is less than or equal to 218mg/kg, and the chromium content is less than or equal to 95mg/kg, meets the national environmental standard requirement, and realizes the repair of lead-chromium-polluted soil. The method is simple, can effectively solve the problems of heavy metal combined pollution, plant survival difficulty and the like, improves soil, improves fertility, promotes crop growth, and is low in cost and environment-friendly.
Description
Technical Field
The invention relates to soil restoration, in particular to a method for restoring lead-chromium composite severely-polluted soil by utilizing humic acid and sudan grass.
Background
The unreasonable application of pesticides and fertilizers causes soil acidification and hardening, and can activate harmful heavy metal elements in soil, so that the content of heavy metals of crops, fruit trees and the like exceeds the standard, and the food safety is affected. Lead and chromium are heavy metals with extremely strong toxicity, are internationally recognized cancerogenic metal substances, and seriously harm ecological environment and human health. At present, established lead and chromium pollution restoration methods mainly comprise physical, chemical, biological and other methods. The common physical and chemical methods have high cost, complex operation and easy secondary pollution. Humic acid is a complex natural polymer organic matter, can improve soil structure, improve soil fertility and stimulate plant growth; in addition, humic acid contains a plurality of active functional groups such as carboxyl, hydroxyl, carbonyl and the like, can generate adsorption, complexation and redox actions with toxic active pollutants and the like in the environment, and changes the occurrence form of heavy metals in soil, thereby achieving the effects of relieving the toxicity of the heavy metals to plants and promoting the growth of the plants. Although many researches at present explore the adsorption-desorption characteristics of humic acid on heavy metals in soil under the influence of various factors, most research objects favor single heavy metals, however, in practice, most soil pollution is composite pollution of multiple heavy metals, and the single repair technology is difficult to solve scientific and technical requirements of complexity, concealment and persistence of heavy metal pollution in China. Although the phytoremediation heavy metal contaminated soil has the advantages of being green, environment-friendly, economical, small in soil disturbance and the like, the phytoremediation heavy metal contaminated soil also has the defects of long plant growth period, small growth amount, difficulty in growing in the soil with serious heavy metal pollution and the like, so that the phytoremediation heavy metal contaminated soil has great development and application potential by assisting the phytoremediation by a technical means.
Although the prior repair of the chromium-polluted soil by utilizing sudan grass is carried out, the actual situation is that most soil pollution is the combined pollution of various heavy metals, such as lead and chromium pollution, and plants which can resist various heavy metals simultaneously are rare, so that the repair rate and efficiency of the heavy metal-polluted soil can be improved, the limitation of a single repair technology can be overcome, and the repair of single or various heavy metal-polluted soil is realized by screening the plants which are resistant to lead and chromium and combining a humic acid and a combined repair system formed by the plants for lead-chromium combined pollution repair, but no disclosure is found up to the present.
Disclosure of Invention
Aiming at the situation, the invention aims to overcome the defects of the prior art, and provides a method for repairing the lead-chromium composite severely polluted soil by utilizing humic acid and sudan grass.
According to the method for repairing the lead-chromium composite severely-polluted soil by utilizing the combination of humic acid and sudan grass, the humic acid is uniformly scattered on the surface layer of the lead-chromium-polluted soil according to the total weight of 100-200 kg/mu, the chromium on the surface layer of the soil is more than or equal to 275.4mg/kg, the lead is more than or equal to 989.7mg/kg, the ploughing soil is 18-22cm deep, raking is flat, ridges are divided, the row spacing between the ridges is 30-40cm, sudan grass seeds are uniformly sown per ridge after 7 days, the sowing amount is 1.5-2 kg/mu, the sowing depth is 2.8-3.2cm, soil covering is carried out, watering is carried out once every 3-7 days in the plant growth process, the sudan grass is grown for 30d, the soil sample is collected for measurement, the lead content of the soil is less than or equal to 218mg/kg, and the chromium content is less than or equal to 95mg/kg, so as to reach the national environmental standard requirement, and the repair of the lead-chromium-polluted soil is realized.
The method is simple, can effectively solve the problems of heavy metal combined pollution, plant survival difficulty and the like, improves soil, improves fertility, promotes crop growth, and has low cost, environmental protection and remarkable economic and social benefits.
Detailed Description
The following describes the embodiments of the present invention in detail with reference to specific cases.
The invention, in its practice, can be illustrated by the following examples.
Example 1
The invention relates to a method for repairing lead-chromium composite severely-polluted soil by utilizing humic acid and sudan grass, which uniformly spreads the humic acid on the surface layer of the lead-chromium-polluted soil according to 150 kg/mu, wherein the surface layer of the soil contains 275.4mg/kg of chromium and 989.7mg/kg of lead, the ploughing depth of the soil is 20cm, the soil is raked and leveled, the ridge is divided, the row spacing between ridges is 35cm, the sudan grass seeds are uniformly sown in each ridge after 7 days, the sowing amount is 1.8 kg/mu, the sowing depth is 3cm, the soil is covered, watering is carried out once every 4-6 days in the plant growth process, the growth of sudan grass is managed according to the normal growth requirement of the sudan grass, the soil sample is collected and measured, the lead content of the soil is less than or equal to 218mg/kg, the chromium content is less than or equal to 95mg/kg, the national environmental standard requirement is met, and the repair of the lead-chromium-polluted soil is realized.
Example 2
The invention relates to a method for repairing lead-chromium composite severely-polluted soil by utilizing humic acid and Sudan grass, which uniformly spreads the humic acid on the surface layer of the lead-chromium-polluted soil according to 110 kg/mu, wherein chromium on the surface layer of the soil is more than or equal to 275.4mg/kg, lead is more than or equal to 989.7mg/kg, the depth of the ploughed soil is 21cm, raking is carried out, ridges are divided, the row spacing between the ridges is 38cm, sudan grass seeds are uniformly sown in each ridge after 7 days, the sowing amount is 1.6 kg/mu, the sowing depth is 2.8cm, soil is covered, watering is carried out once every 4-5 days in the plant growth process, management is carried out according to the normal growth requirement of the Sudan grass, the Sudan grass is grown for 30d, a soil sample is collected and measured, the lead content of the soil is less than or equal to 218mg/kg, the chromium content is less than or equal to 95mg/kg, the national environmental standard is met, and the repair of the lead-chromium-polluted soil is realized.
Example 3
The invention relates to a method for repairing lead-chromium composite severely-polluted soil by utilizing humic acid and sudan grass, which uniformly spreads the humic acid on the surface layer of the lead-chromium-polluted soil according to 180 kg/mu, wherein chromium on the surface layer of the soil is more than or equal to 275.4mg/kg, lead is more than or equal to 989.7mg/kg, the ploughing depth of the soil is 19cm, raking is carried out, ridges are divided, the row spacing between the ridges is 32cm, sudan grass seeds are uniformly sown in each ridge after 7 days, the sowing amount is 1.9 kg/mu, the sowing depth is 3.2cm, soil is covered, watering is carried out once every 6-7 days in the plant growth process, management is carried out according to the normal growth requirement of sudan grass, the sudan grass is grown for 30d, a soil sample is collected and measured, the lead content of the soil is less than or equal to 218mg/kg, the chromium content is less than or equal to 95mg/kg, the national environmental standard is met, and the repair of the lead-chromium-polluted soil is realized.
The invention has very good effect through field tests and tests, and the related data are as follows:
1. screening test of lead-chromium-resistant plants
Spreading the primary screening plant seeds with approximately the same plumpness in a culture dish with the diameter of 3.5cm, putting 3mL of prepared primary chromium solution (lead solution) into each dish to treat the primary screening plants, taking various plants treated by clear water as blank Control (CK), watering regularly, culturing for 5-7d under the constant temperature condition of 25 ℃, and observing the growth condition of the primary screening plants; and selecting plants with higher relative germination rate and better growth vigor to perform re-screening with slightly higher concentration, wherein the method is the same as above until plants with stronger lead-chromium resistance are respectively screened out. The screening concentration of the chromium solution is respectively 50mg/L, 100mg/L, 150mg/L, 200mg/L, 300 mg/L, 400mg/L and 600mg/L.
Collecting 16 plants at the initial stage as primary screening plants, treating the primary screening plants with 50mg/L chromium solution, culturing for 5-7d, selecting plants with better growth vigor for 100mg/L chromium solution treatment and re-screening according to the statistical result of relative germination rate, and finding that 4 plants with better growth vigor in 100mg/L chromium solution are respectively: pennisetum, hybrid sorghum, sudan grass and Convolvulus arvensis. Treating the four plants with 150mg/L and 200mg/L chromium solution respectively to finally obtain two plants with stronger chromium resistance, namely sudan grass and hybrid sorghum respectively; treating the primary screening plants with 200mg/L lead solution, culturing for 3-5d, selecting plants with better growth vigor, carrying out 400mg/L lead solution treatment and rescreening, and finding 5 plants with stronger 600mg/L lead tolerance according to the relative germination rate statistical result and growth vigor of various plants, wherein the 5 plants are respectively: pennisetum, hybrid sorghum, sudangrass, patrinia, convolvulus arvensis, preferably sudangrass. Therefore, sudan grass is selected as the lead-chromium resistant plant.
2. And (5) repairing the lead-chromium polluted soil.
Taking example 1 as an example, 3 repeated tests are carried out, and the addition of humic acid can improve the fertility of soil. Under the combined action of humic acid and sudan grass, the quick-acting phosphorus content in the lead-chromium composite polluted soil is increased by 20.72 to 23.20 percent compared with that of blank polluted soil (i.e. no humic acid is added and sudan grass is planted), and is increased by 3.78 to 6.23 percent compared with that of single sudan grass planting treatment; under the combined action of humic acid and sudan grass, the quick-acting potassium content in the lead-chromium polluted soil is increased by 7.75-13.31 percent compared with that of blank polluted soil, and is increased by 3.07-8.39 percent compared with the treatment of solely planting sudan grass.
The humic acid treatment can promote the growth of Sudan grass and improve the biomass thereof. Compared with the treatment of solely planting the sudan grass, the plant height of the sudan grass is increased by 3.33-3.67 cm under the humic acid treatment, the fresh weight of the overground part of the sudan grass is increased by 22.23-30.96%, and the fresh weight of the underground part is increased by 9.05-21.76%; the dry weight of the overground part of the Sudan grass is increased by 32.73-38.38 percent, and the dry weight of the underground part is increased by 4.53-10.08 percent.
After the humic acid and the sudan grass act together for 30d, the content of heavy metal chromium in the soil is reduced to 94.4mg/kg from the original chromium not less than 275.4mg/kg, the content of lead is reduced to 217.7mg/kg from the original 989.7mg/kg, the effect is very good, and the soil environment quality standard specified by the state is completely reached.
The test is carried out while the test is carried out, and 3 repeated tests are carried out on the embodiment 2 and the embodiment 3 respectively, and the same and similar results are obtained, so that the method is stable and reliable, the effect is good, after the test is carried out, the humic acid and the sudan grass act together for 30 days, the heavy metal chromium in the soil is reduced by 65.71-75.85%, the lead is reduced by 78.71-85.81%, and the soil quality can meet the national environmental standard requirement:
GB15618-1995
sudan grass is used as a repair plant, and is characterized by developed fibrous roots, high growth speed, good regeneration, strong leaf separation property and strong stress resistance, and is suitable for repairing lead-chromium polluted soil. The Sudan grass has wide adaptability, can be cultivated in a plurality of areas, has wide application soil range, can be cultivated in clay, sandy loam, slightly acidic and slightly alkaline soil, has low cost for repairing plants, is easy to develop and popularize, can be used as silage, saves resources, and has the characteristics of strong adaptability, rapid growth, good reproducibility, strong resistance, strong soil fixation, easier later recycling and the like, and is more and more favored by people as heavy metal repairing plants, thereby having good repairing effect. Compared with the prior art, the method has the following outstanding technical effects:
1. the problem that plants are difficult to survive under the condition of lead-chromium composite polluted soil can be solved;
2. humic acid can improve soil, improve soil fertility and increase plant growth, thereby improving the restoration efficiency of the plant to heavy metal composite polluted soil, and the restoration period is short and can be improved by more than 50%;
3. the method has the advantages of no pollution, no public hazard, environmental protection, convenient operation and low cost, which is only less than 1/3 of the cost of the prior art, and has remarkable economic and social benefits.
Claims (4)
1. A method for repairing lead-chromium composite severely-polluted soil by utilizing humic acid and sudan grass is characterized in that the humic acid is uniformly scattered on the surface layer of the lead-chromium-polluted soil according to the ratio of 100-200 kg/mu, the chromium on the surface layer of the soil is more than or equal to 275.4mg/kg, the lead is more than or equal to 989.7mg/kg, the ploughing depth of the soil is 18-22cm, the soil is raked and leveled, ridges are separated, the row spacing between the ridges is 30-40cm, sudan grass seeds are uniformly sown in each ridge after 7 days, the sowing amount is 1.5-2 kg/mu, the sowing depth is 2.8-3.2cm, the soil is covered, watering is carried out once every 3-7 days in the plant growth process, the sudan grass grows for 30d, the soil sample is collected for measurement, the lead content of the soil is less than or equal to 218mg/kg, and the chromium content is less than or equal to 95mg/kg.
2. The method for repairing the lead-chromium composite severely-polluted soil by utilizing humic acid and sudan grass in a combined way, which is disclosed in claim 1, is characterized in that the humic acid is uniformly scattered on the surface layer of the lead-chromium-polluted soil according to 150 kg/mu, the surface layer of the soil contains 275.4mg/kg of chromium and 989.7mg/kg of lead, the depth of the ploughed soil is 20cm, the soil is raked and leveled, ridges are divided, the row spacing between the ridges is 35cm, the sudan grass seeds are uniformly sown in each ridge after 7 days, the sowing amount is 1.8 kg/mu, the sowing depth is 3cm, the soil is covered, the soil is watered once every 4-6 days in the plant growth process, the growth of the sudan grass is managed according to the normal growth requirement of the sudan grass, the soil sample is collected and measured, the lead content of the soil is less than or equal to 218mg/kg, and the chromium content is less than or equal to 95mg/kg.
3. The method for repairing the lead-chromium composite severely-polluted soil by utilizing humic acid and sudan grass in a combined way, which is disclosed in claim 1, is characterized in that the humic acid is uniformly scattered on the surface layer of the lead-chromium-polluted soil according to the proportion of 110 kg/mu, the chromium on the surface layer of the soil is more than or equal to 275.4mg/kg, the lead is more than or equal to 989.7mg/kg, the depth of the ploughed soil is 21cm, the soil is raked and leveled, ridges are divided, the row spacing between the ridges is 38cm, the sudan grass seeds are uniformly sown in each ridge after 7 days, the sowing amount is 1.6 kg/mu, the sowing depth is 2.8cm, the soil is covered, the soil is watered once every 4-5 days in the plant growth process, the sudan grass is grown for 30d according to the normal growth requirement of the sudan grass, the lead content of the soil is less than or equal to 218mg/kg, and the chromium content is less than or equal to 95mg/kg.
4. The method for repairing the lead-chromium composite severely-polluted soil by utilizing humic acid and sudan grass in a combined way, which is disclosed in claim 1, is characterized in that the humic acid is uniformly scattered on the surface layer of the lead-chromium-polluted soil according to 180 kg/mu, the chromium on the surface layer of the soil is more than or equal to 275.4mg/kg, the lead is more than or equal to 989.7mg/kg, the depth of the ploughed soil is 19cm, the soil is raked and leveled, ridges are divided, the row spacing between the ridges is 32cm, the sudan grass seeds are uniformly sown in each ridge after 7 days, the sowing amount is 1.9 kg/mu, the sowing depth is 3.2cm, the soil is covered, the sudan grass is watered once every 6-7 days in the plant growth process, the sudan grass is grown for 30d according to the normal growth requirement of the sudan grass, the soil lead content is less than or equal to 218mg/kg, and the chromium content is less than or equal to 95mg/kg.
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JP2004130199A (en) * | 2002-10-09 | 2004-04-30 | Mitsui Mining & Smelting Co Ltd | Method and system for repairing heavy metal-polluted soil |
CN102319729A (en) * | 2011-07-30 | 2012-01-18 | 台州职业技术学院 | New method for combined remediation of compound contaminated soil |
CN105195507A (en) * | 2015-10-23 | 2015-12-30 | 河南省科学院生物研究所有限责任公司 | Method for performing combined remediation on chromium-contaminated soil by utilizing sudan grass and high effective microbes |
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