CN110876691A

CN110876691A - Coenzyme Q10Sleep-aiding skin-beautifying massage emulsion

Info

Publication number: CN110876691A
Application number: CN201911038985.6A
Authority: CN
Inventors: 吴铁; 唐林志; 于琼; 吕思敏; 丁鸿燕; 丁喜生
Original assignee: GUANGDONG RUNHE BIOTECHNOLOGY Co Ltd; Guangdong Medical University
Current assignee: GUANGDONG RUNHE BIOTECHNOLOGY Co Ltd; Guangdong Medical University
Priority date: 2019-10-29
Filing date: 2019-10-29
Publication date: 2020-03-13

Abstract

The invention discloses coenzyme Q₁₀And melatonin in preparing external massage preparation for improving sleep and caring skin. The research finds that the coenzyme Q₁₀The melatonin is added into massage cream, massage cream and massage oil according to a certain proportion, so that the skin-beautifying effect and the sleep-aiding effect are achieved, and the addition amount of the two is every 100 g of cream or every 100 g of creamCoenzyme Q in 100 ml of oil solution₁₀And melatonin are preferably added in an amount of 0.1 to 5.0g, with the optimal ratio of the two being 1: 6. The invention also discloses the formulation and preparation process of 5 external preparations of massage cream, massage cream and massage oil containing the two components. When the external massage preparation is applied, a small amount of the external massage preparation is smeared on the skin, and then the skin is slightly massaged or is massaged in cooperation with acupuncture points, so that the sleep can be quickly induced, the sleep time is shortened, the sleep quality is improved, and the external massage preparation has good skin moistening, repairing and whitening effects on the skin. Animal experiment results also prove that the external massage preparation has the function of helping sleep.

Description

Coenzyme Q10Sleep-aiding skin-beautifying massage emulsion

Technical Field

The invention relates to a group of coenzyme Q₁₀The external massage preparation with the functions of helping sleep and beautifying skin comprises massage milk, massage cream and massage oil, and has better sleep-helping function for people with mild sleep disorder and insomnia. When the skin care product is applied, a small amount of the skin care product is smeared on the skin, and then the skin care product is slightly massaged or matched with acupuncture point massage, so that the sleep can be quickly induced, the sleep time is shortened, the sleep quality is improved, and the skin care product has good skin moistening, repairing and whitening effects on the skin, and belongs to the technical field of external pharmaceutical preparations and skin care products.

Background

Insomnia is common in daily life, and almost every adult experiences insomnia, and the common manifestations are difficulty in falling asleep, sleep quality reduction, sleep time reduction, memory and attention reduction, and the like. Modern medicine has limitations on the understanding of insomnia, but clinical physicians have begun to define insomnia according to clinical studies, and the neurological society sleep disorder group of the Chinese medical society in 2012 made "Chinese adult insomnia diagnosis and treatment guidelines" according to the existing evidence of evidence, wherein insomnia refers to a subjective experience of a patient that the sleep time and/or quality are not satisfied and the daily social function is affected. Insomnia can be classified into primary and secondary types according to its etiology. (1) Primary insomnia: mainly comprises 3 types of psychophysiological insomnia, idiopathic insomnia and subjective insomnia. The primary insomnia usually lacks a definite cause or still remains insomnia symptoms after eliminating causes which may cause insomnia, and the diagnosis lacks a specific index, which is mainly an exclusive diagnosis. Primary insomnia is considered to be caused when insomnia symptoms remain after the etiology that may cause insomnia is eliminated or cured. According to the psychological and physiological insomnia, the etiology can be traced to the influence of a certain or long-term event on the functional stability of the peripheral system of the brain of a patient, and the imbalance of the functional stability of the peripheral system finally causes the disorder of the brain sleep function and the insomnia. (2) Secondary insomnia: including insomnia due to physical diseases, mental disorders, drug abuse, etc., and insomnia associated with sleep disordered breathing, sleep movement disorders, etc. Insomnia frequently occurs simultaneously with other diseases, and sometimes it is difficult to determine the causal relationship between these diseases and insomnia, so in recent years the concept of comorbidinsomnia has been proposed to describe those insomnia that are accompanied simultaneously with other diseases.

Methods of treatment for insomnia include drug therapy and non-drug therapy. The clinical medicine mainly takes drug therapy as the main part, and the drugs for clinically treating insomnia at present mainly comprise benzodiazepine receptor agonists, melatonin receptor agonists and antidepressant drugs with hypnotic effect. Although antihistamine (such as diphenhydramine), melatonin and valerian extract have hypnotic effect, the existing clinical research evidence is limited, and the extract is not suitable for conventional insomnia medication. The drugs for treating insomnia are complex and numerous and include estazolam, flurazepam, quazepam, temazepam, triazolam, alprazolam, chlordiazepoxide, diazepam, lorazepam, midazolam, zolpidem controlled release agents, zopiclone, eszopiclone and zaleplon, ramelten, agomelatine, tricyclic antidepressants, selective 5-tryptamine reuptake inhibitors (SSRIs), 5-hydroxytryptamine and noradrenaline reuptake inhibitors (SNRIs), small doses of mirtazapine, small doses of trazodone, and the like. Because of the possibility of some drugs being dependent, long-term administration is generally not advocated.

Melatonin is a hormone substance capable of lightening skin pigment, which is synthesized by mammals and humans in pineal gland with tryptophan as matrix and has chemical name of N-acetyl, 5-methoxytryptamine. The physiological effects includeRegulating biological clock, inducing sleep, regulating endocrine system, delaying aging, and enhancing immunity, is called "Physiological catalysis Sleep improving agent". The exogenous administration of melatonin can shorten the wake-up time before sleep and the sleep-in time, improve the sleep quality, obviously reduce the wake-up times in sleep, shorten the light sleep stage, prolong the deep sleep stage and reduce the wake-up threshold in the next morning. Has stronger function of adjusting time difference. The biological functions of melatonin, especially the health care function as a dietary supplement, are widely researched at home and abroad, and the melatonin has multiple physiological functions of promoting sleep, regulating jet lag, resisting aging, regulating immunity, resisting tumors and the like. The health care function of melatonin at home and abroad relates to the regulation of endocrine (ovulation inhibition), the protection of encephalitis virus infection, the reduction of death rate after infection, the treatment of AIDS, the protection of cardiovascular and the like. The oral melatonin is proved to improve the sleep quality and have the treatment effect on insomnia, particularly can regulate the rhythm of sleep, has no phenomena of dizziness and somnolence on the next day, and has no obvious side effect even if being taken in large dose for a long time. Because the half-life period of melatonin is too short and the blood concentration fluctuation is large, although the melatonin can effectively induce sleep before taking, the effect on prolonging the sleep time is unstable, so the melatonin is mainly clinically used for treating sleep disorder in the form of a health-care product at present, and meanwhile, the sustained-release preparation is actively developed. Melatonin can resist melanin in skin, so that the melatonin is approved to be used in skin care products for whitening and moisturizing, and researches show that the melatonin has good bioavailability when being applied through skin, and the medicament can generate good sustained and controlled release effects when being applied through skin and can solve the problem of short half-life period of the melatonin. Our research finds that melatonin can be absorbed through skin, and when the melatonin is externally applied to reach a certain amount, a sleep-aiding effect can also occur. The sleep-aiding massage cream with various concentrations is prepared, an in-vitro transdermal experiment is carried out on the skin of a mouse, and an overall transdermal experiment research of a depilated mouse is also carried out, so that the melatonin can play a transdermal role only under a relatively large dosage. When the dosage is converted into cream, the cream can play a good sleep-aiding role until more than 3-5 g of the cream is contained in every 100 g of the cream. Further research shows that melatonin is mixed with coenzyme Q₁₀The combined application can also exert better sleep-aiding effect even if a small dosage of melatonin is applied.

Coenzyme Q₁₀Is a coenzyme, coenzyme Q, which can be synthesized and updated by human body₁₀Coenzyme Q has been widely used in clinic as a fat-soluble antioxidant₁₀Can activate human cells and nutrition of cell energy, has the functions of improving human immunity, enhancing oxidation resistance, delaying senility, enhancing human vitality and the like, is proved in the aspects of prevention and treatment of diseases such as cardiovascular system and the like, and is widely used as a nutritional health product and a food additive at home and abroad. Coenzyme Q₁₀The beneficial effects on the skin have also been confirmed, with the skin collagen having a reduced ability to withstand damage from oxidative stimuli such as ultraviolet light with age, and coenzyme Q has been used for a long period of time₁₀Can effectively prevent skin photoaging and reduce wrinkles around eyes due to coenzyme Q₁₀The skin growth layer can weaken the oxidation reaction of photons, can start specific phosphorylated tyrosine kinase with the help of tocopherol, prevent the oxidative damage of DNA, inhibit the expression of human skin fibroblast collagenase under the irradiation of ultraviolet light, and protect the skin from being damaged. Extensive research suggests that coenzyme Q₁₀Inhibiting lipid peroxidation, reducing free radical generation, protecting SOD active center and its structure from free radical oxidation damage, improving in vivo SOD enzyme activity, inhibiting oxidative stress induced apoptosis, and resisting oxidation and delaying aging. Our observations of prolonged administration of coenzyme Q₁₀The people have better sleep and have less insomnia, which is combined with coenzyme Q₁₀In connection with a beneficial effect on the human nervous system, we have also observed that coenzyme Q₁₀When taken together with melatonin, the medicine has better sleep effect and certain curative effect on general insomnia. Further, we found that coenzyme Q₁₀The melatonin and the melatonin are combined to form a massage cream, people with poor sleep are massaged before sleeping, the massage cream also has a good sleep promoting effect, the two components are subjected to composition research through animal experiments, and the best massage cream is found through combination of the two components in different proportionsThe best coenzyme Q group is finally found₁₀The invention discloses a formula and a production process of melatonin massage cream, and accordingly, the invention is reported.

Disclosure of Invention

Our research found that coenzyme Q₁₀Coenzyme Q, a beneficial effect on the human sleep-related nervous system₁₀When taken together with melatonin, the medicine has better sleep effect and certain curative effect on insomnia. Further research finds that the coenzyme Q₁₀The melatonin is added into the externally applied massage cream together, people with poor sleep are massaged before sleeping, and the massage cream also has a good sleep promoting effect, so that the invention provides a group of coenzyme Q₁₀And melatonin, and can be used as massage cream for skin care and sleep, including cream, emulsion and oil. Adding coenzyme Q into the base of externally applied ointment, cream, emulsion and oil preparation prepared conventionally₁₀And melatonin are prepared into various preparations for clinical external use, and the preparations can be prepared into products with the effect of inducing sleep. The formulation is characterized by containing coenzyme Q₁₀Melatonin component in 100 ml cream or 100 ml oil solution and coenzyme Q₁₀The content of (A) is 0.1-5.0 g, the content of melatonin is 1.0-5.0 g, and the optimal ratio of the two is 1: 6.

The specific implementation mode is as follows:

the following are specific embodiments of the present invention and further description of the technical solutions of the present invention, but the scope of the present invention is not limited to these embodiments, and all changes or equivalent substitutions that do not depart from the spirit of the present invention are included in the scope of the present invention.

Example one

Coenzyme Q₁₀The formula of the sleep-aiding milk comprises (100 g): coenzyme Q₁₀0.1 to 5.0g, melatonin 1.0 to 5.0g, tyrosine 1.0 to 5.0g, dipotassium glycyrrhizinate 0.1 to 1.0 g, menthol 0.1 to 1.0 g, almond oil 10 to 20g, vitamin B60.1 to 1.0 g, glycerin 2.0 to 5.0g, azone 0.5 to E3.0g of lavender essential oil, 0.1-1.0 g of lavender essential oil, 5.0-10 g of emulsifier, 1.0-5.0 g of ethanol, and the balance of distilled water to make up to 100 g.

Dissolving melatonin with glycerol, adding ethanol, Mentholum, azone, and lavender essential oil, and mixing to obtain liquid A; dissolving tyrosine, dipotassium glycyrrhizinate, vitamin B6 and phenoxyethanol in distilled water to obtain a liquid B; fully and uniformly mixing the liquid A and the liquid B to obtain liquid C; adding coenzyme Q₁₀Mixing with oleum Armeniacae amarum emulsifier, heating to dissolve to obtain liquid D; and fully and uniformly mixing the liquid C and the liquid D, emulsifying and uniformly mixing to obtain a finished product.

Coenzyme Q₁₀The optimal formula proportion of the sleep-aiding milk is as follows: coenzyme Q₁₀0.5 g, 3.0g of melatonin, 3.0g of tyrosine, 0.3 g of dipotassium glycyrrhizinate, 0.5 g of menthol, 17 g of almond oil, 60.3 g of vitamin B and 10g of glycerol; 3.0g of azone, 0.5 g of lavender essential oil, 8.0 g of emulsifier, 2.0 g of ethanol and the balance of distilled water to make up to 100 g.

Example two

Coenzyme Q₁₀The formula of the sleep-aiding cream comprises (100 g): coenzyme Q₁₀0.1-5.0 g of melatonin, 1.0-5.0 g of white oil, 20-25 g of white oil, 8.0-12.0 g of beeswax, 20-25 g of isopropyl myristate, 0.1-0.2 g of borax, 5-10 g of liquid paraffin, 2.0-3.0 g of P0E (25) sorbitan monoester, 12-15 g of vaseline, 2.0-3.0 g of glyceryl monostearate, 60.1-1.0 g of vitamin B, 1.0-5.0 g of tyrosine, 0.1-1.0 g of dipotassium glycyrrhizinate and 2-3 g of glycerol; 2-3 g of propylene glycol; 0.5-1 g of azone, a proper amount of essence and the balance of distilled water, wherein the balance is up to 100 g.

The preparation method comprises the following steps: taking coenzyme Q₁₀Adding melatonin into the oil phase component, mixing and heating to 75 deg.C; mixing dipotassium glycyrrhizinate, tyrosine and water phase components, and heating to 75 deg.C; slowly adding the oil phase component into the water phase component, emulsifying, cooling to 40 deg.C, adding vitamin B6 and essence, and stirring to obtain the final product.

EXAMPLE III

Coenzyme Q₁₀The formula of the sleep-aiding paste comprises (100 g): coenzyme Q₁₀0.1-5.0 g, fading black1.0-5.0 g of vitamin, 1.0-5.0 g of tyrosine, 0.1-1.0 g of dipotassium glycyrrhizinate, 60.1-1.0 g of vitamin B, 0.01-0.05 g of lipoic acid and 6-10 g of liquid paraffin; 2-4 g of polyoxyethylene (2) stearyl alcohol ether; 2-4 g of isopropyl myristate; 1-3 g of lanolin; 1-3 g of vaseline; 1-3 g of monoglyceride; 1-3 g of hexadecanol; 1-3 g of almond oil; 0.02-0.1 g of candelilla wax; 0.1-0.1 g of ethylparaben, 3-4 g of polyoxyethylene (21) stearyl ether; 2-3 g of glycerol; 2-3 g of propylene glycol; 0.5-1 g of azone; proper amount of essence and the balance of distilled water are added to make up to 100 g.

The preparation method comprises the following steps: taking coenzyme Q₁₀Adding melatonin into the oil phase component, mixing and heating to 75 deg.C; mixing dipotassium glycyrrhizinate, tyrosine and lipoic acid with water phase components, and heating to 75 deg.C; slowly adding the oil phase component into the water phase component, emulsifying, cooling to 40 deg.C, adding vitamin B6 and essence, and stirring to obtain the final product.

Example four

Coenzyme Q₁₀The formula of the sleep-aiding massage oil (I) comprises the following components (100 ml): coenzyme Q₁₀0.1-8.0 g of melatonin, 1.0-5.0 g of tyrosine, 0.1-1.0 g of dipotassium glycyrrhizinate, 60.1-1.0 g of vitamin B, 0.01-0.05 g of lipoic acid, 30-35 g of almond oil, 40-50 g of liquid paraffin and 2-3 g of glycerol; 2-3 g of propylene glycol; 0.5-1 g of azone and a proper amount of essence.

The preparation method comprises the following steps: slowly heating all the materials except essence under stirring until all the materials are dissolved, and adding essence when the temperature of the materials is reduced to 45 ℃.

EXAMPLE five

Coenzyme Q₁₀The formula of the sleep-aiding skin-beautifying massage oil (II) comprises the following components (100 milliliters): coenzyme Q₁₀0.1-8.0 g of melatonin, 1.0-5.0 g of tyrosine, 0.1-1.0 g of dipotassium glycyrrhizinate, 60.1-1.0 g of vitamin B, 0.01-0.05 g of lipoic acid, 40-45 g of isopropyl myristate, 40-45 g of caprylic/capric triglyceride, 8-10 g of silicone oil and 2-3 g of glycerol; 2-3 g of propylene glycol; 0.5-1 g of azone and a proper amount of essence.

Evaluation of animal experiments

To observe coenzyme Q₁₀The curative effect of the sleep-aiding milk is that animal experiments are carried out on products prepared by the formulas according to the pharmacodynamic evaluation method, and preliminary research results are obtained, which are briefly described as follows.

Experiment 1 coenzyme Q₁₀Observation of the sleep-aid milk on the autonomous movement of the mouse: taking 18-20 g of normal mice, removing hair from the center of the back of the mice, randomly dividing into 3 groups, each group containing 10 mice, and the 1 st group as sleep-promoting emulsion blank control group, i.e. the emulsion does not contain coenzyme Q₁₀And melatonin, the other components are the same, group 2 is melatonin milk-aid group, and the group does not contain coenzyme Q₁₀. Group 3 is coenzyme Q₁₀And melatonin sleep-aid milk group, namely the formula group of the first embodiment; in the experiment, 30 drops of corresponding emulsion are smeared on the dead center of the back of each group of mice according to groups, and the mice are massaged and absorbed within 5 minutes. After the massage is finished, the mouse is placed into an observation cage, the subsequent mouse activity condition within 65 minutes is recorded, the exercise frequency of each mouse within 50-60 minutes is counted (the two forelimbs of the mouse are counted off the ground simultaneously), and the result shows that the group 3 contains coenzyme Q (shown in table 1)₁₀The composition has obvious inhibition effect on the autonomous movement of mice with the melatonin milk-aid group, and the group 2 only contains the melatonin milk-aid group also has a certain reduction effect, but the effect is not obvious.

TABLE 1 coenzyme Q₁₀Observations of the sleep-aid milk on the voluntary movements of the mice are as follows.

Experiment 2 coenzyme Q₁₀Observation of sleep-aid milk on mouse suprathreshold pentobarbital sodium sleep test: taking 18-20 g of normal mice, feeding for two weeks, fasting for 8 hours at 7 am during experiment, randomly dividing the mice into 3 groups, each group containing 10 mice, using the same medicine as experiment 1, and taking group 1 as sleep-aid milkBlank control group, group 2 is melatonin milk for improving sleep, and the group does not contain coenzyme Q₁₀. Group 3 is coenzyme Q₁₀And melatonin sleep-promoting milk group; the experiment was started by slowly dropping 15 drops of cream on the skin depilated on the back and massaging for absorption within 3 min. 50min after smearing administration, each group of mice is 45 mg/kg^-1Pentobarbital sodium (0.1 mL/10g by weight) is intraperitoneally injected with BW dosage, the injection time (T.R) is recorded, when the inversion positive reflex disappears for 1min within 15min, the recording time indicates that the mouse falls asleep (T.S), when the inversion positive reflex recovers, the recording time indicates that the mouse sleeps and wakes up (T.W). The ① sleep latency (T.S-T.R), the time indicating that the mouse disappears from the administration to the inversion positive reflex, the ② sleep duration (T.W-T.S), the time indicating that the mouse disappears from the inversion positive reflex to the inversion positive reflex recovery are respectively calculated according to the indexes, the experimental results can be seen (see table 2), the 3 rd group contains coenzyme Q₁₀The sleep-aid milk with the melatonin can effectively prolong the sleep time of the pentobarbital and shorten the latency time, and the effect of independently applying the melatonin is not obvious.

TABLE 2 coenzyme Q₁₀The observation of the sleep-aid milk on the mouse suprathreshold sodium pentobarbital sleep test is as follows.

Note: p <0.05 compared to the first group; p <0.01 compared to the first group.

Volunteer evaluations were as follows.

Case 1: li in Li, 57 years old, is difficult to fall asleep, easy to wake up after sleeping, and difficult to fall asleep again after waking up. The product is applied to the skin of ears, forehead, etc., to make the skin feel cool and comfortable, make it easy to fall asleep, prolong sleep time, improve wakefulness, and keep good in state the next day.

Case 2: in 37 years old, sleep is usually adequate at ordinary times, but after 1 o' clock, the mouth is dry and bitter, and it is difficult to fall asleep. The product is applied to the skin of the back of the ear, forehead and nape, and the skin is cool and comfortable, people feel relaxed, the mouth is dry, the tongue is bitter, the product can be improved, the product can sleep in about 20min, and no phenomena of dizziness, difficulty in sleeping and the like exist in the next day.

Case 3: qiu Zhi, 65 years old, insomnia, poor sensation and dreaminess, a proper amount of the product is taken and smeared on the skin, the acupuncture points are slightly massaged, and the user can sleep and sense at night basically.

Claims

1. Coenzyme Q₁₀The application of the melatonin composition in preparing an external massage preparation with the functions of helping sleep and beautifying skin.

2. The coenzyme Q according to claim 1₁₀The application of the melatonin composition in preparing an external massage preparation with the functions of helping sleep and beautifying skin is characterized in that the preparation is massage cream, massage cream and massage oil.

3. The coenzyme Q according to claim 1₁₀The application of the melatonin composition in preparing an external massage preparation with the functions of helping sleep and beautifying skin is characterized in that the preparation method of the massage emulsion comprises the following steps:

(1) the raw materials comprise: coenzyme Q₁₀0.1-5.0 g of melatonin, 1.0-5.0 g of tyrosine, 1.0-5.0 g of dipotassium glycyrrhizinate, 0.1-1.0 g of menthol, 10-20 g of almond oil, 60.1-1.0 g of vitamin B, 2.0-5.0 g of glycerol, 0.5-3.0 g of azone, 0.1-1.0 g of lavender essential oil, 5.0-10 g of emulsifier, 1.0-5.0 g of ethanol, and the balance of distilled water which is supplemented to 100 g;

(2) the preparation method comprises the following steps: dissolving melatonin with glycerol, adding ethanol, Mentholum, azone, and lavender essential oil, and mixing to obtain liquid A; dissolving tyrosine, dipotassium glycyrrhizinate, vitamin B6 and phenoxyethanol in distilled water to obtain a liquid B; fully and uniformly mixing the liquid A and the liquid B to obtain liquid C; then mixing coenzyme Q10 and almond oil emulsifier, heating and dissolving to obtain liquid D; and fully and uniformly mixing the liquid C and the liquid D, emulsifying and uniformly mixing to obtain a finished product.

4. The coenzyme Q according to claim 1₁₀The application of the composition and the melatonin in preparing the external massage preparation with the functions of helping sleep and beautifying skin is characterized in that the massage creamThe preparation method comprises the following steps:

(1) the raw materials comprise: coenzyme Q₁₀0.1-5.0 g of melatonin, 1.0-5.0 g of white oil, 20-25 g of white oil, 8.0-12.0 g of beeswax, 20-25 g of isopropyl myristate, 0.1-0.2 g of borax, 5-10 g of liquid paraffin, 2.0-3.0 g of P0E (25) sorbitan monoester, 12-15 g of vaseline, 2.0-3.0 g of glyceryl monostearate, 60.1-1.0 g of vitamin B, 1.0-5.0 g of tyrosine, 0.1-1.0 g of dipotassium glycyrrhizinate and 2-3 g of glycerol; 2-3 g of propylene glycol; 0.5-1 g of azone, a proper amount of essence and the balance of distilled water, wherein the balance is the balance, and the weight is 100 g;

(2) the preparation method comprises the following steps: taking coenzyme Q₁₀Adding melatonin into the oil phase component, mixing and heating to 75 deg.C; mixing dipotassium glycyrrhizinate, tyrosine and water phase components, and heating to 75 deg.C; slowly adding the oil phase component into the water phase component, emulsifying, cooling to 40 deg.C, adding vitamin B6 and essence, and stirring to obtain the final product.

5. The coenzyme Q according to claim 1₁₀The application of the melatonin composition in preparing an external massage preparation with the functions of helping sleep and beautifying skin is characterized in that the preparation method of the massage cream is as follows:

(1) the raw materials comprise: coenzyme Q₁₀0.1-5.0 g of melatonin, 1.0-5.0 g of tyrosine, 0.1-1.0 g of dipotassium glycyrrhizinate, 60.1-1.0 g of vitamin B, 0.01-0.05 g of lipoic acid and 6-10 g of liquid paraffin; 2-4 g of polyoxyethylene (2) stearyl alcohol ether; 2-4 g of isopropyl myristate; 1-3 g of lanolin; 1-3 g of vaseline; 1-3 g of monoglyceride; 1-3 g of hexadecanol; 1-3 g of almond oil; 0.02-0.1 g of candelilla wax; 0.1-0.1 g of ethylparaben, 3-4 g of polyoxyethylene (21) stearyl ether; 2-3 g of glycerol; 2-3 g of propylene glycol; 0.5-1 g of azone; proper amount of essence, and the balance of distilled water to 100 g;

(2) the preparation method comprises the following steps: taking coenzyme Q₁₀Adding melatonin into the oil phase component, mixing and heating to 75 deg.C; mixing dipotassium glycyrrhizinate, tyrosine and lipoic acid with water phase components, and heating to 75 deg.C; mixing oilSlowly adding the phase components into the water phase components, emulsifying, cooling to 40 deg.C, adding vitamin B6 and essence, and stirring to obtain the final product.

6. The coenzyme Q according to claim 1₁₀The application of the composition and melatonin in preparing an external massage preparation with the effects of helping sleep and beautifying skin is characterized in that the preparation method of the sleep-helping massage oil comprises the following steps:

(1) the raw materials comprise: coenzyme Q₁₀0.1-8.0 g of melatonin, 1.0-5.0 g of tyrosine, 0.1-1.0 g of dipotassium glycyrrhizinate, 60.1-1.0 g of vitamin B, 0.01-0.05 g of lipoic acid, 30-35 g of almond oil, 40-50 g of liquid paraffin and 2-3 g of glycerol; 2-3 g of propylene glycol; 0.5-1 g of azone and a proper amount of essence;

(2) the preparation method comprises the following steps: slowly heating all the materials except essence under stirring until all the materials are dissolved, and adding essence when the temperature of the materials is reduced to 45 ℃.

7. The coenzyme Q according to claim 1₁₀The application of the melatonin composition in preparing an external massage preparation with the functions of helping sleep and beautifying skin is characterized in that the preparation method of the massage oil for helping sleep and beautifying skin comprises the following steps:

(1) the raw materials comprise: coenzyme Q₁₀0.1-8.0 g of melatonin, 1.0-5.0 g of tyrosine, 0.1-1.0 g of dipotassium glycyrrhizinate, 60.1-1.0 g of vitamin B, 0.01-0.05 g of lipoic acid, 40-45 g of isopropyl myristate, 40-45 g of caprylic/capric triglyceride, 8-10 g of silicone oil and 2-3 g of glycerol; 2-3 g of propylene glycol; 0.5-1 g of azone and a proper amount of essence;

8. The coenzyme Q according to claim 1₁₀Application of melatonin composition in preparing external massage preparation with effects of improving sleep and beautifying skinCharacterized in that the optimal formula of the massage milk is as follows: coenzyme Q₁₀0.5 g, 3.0g of melatonin, 3.0g of tyrosine, 0.3 g of dipotassium glycyrrhizinate, 0.5 g of menthol, 12 g of almond oil, 60.3 g of vitamin B and 10g of glycerol; 3.0g of azone, 0.5 g of lavender essential oil, 8.0 g of emulsifier, 2.0 g of ethanol and the balance of distilled water to make up to 100 g.