CN110876310A - Agilawood preparation method and agilawood - Google Patents
Agilawood preparation method and agilawood Download PDFInfo
- Publication number
- CN110876310A CN110876310A CN201910918996.7A CN201910918996A CN110876310A CN 110876310 A CN110876310 A CN 110876310A CN 201910918996 A CN201910918996 A CN 201910918996A CN 110876310 A CN110876310 A CN 110876310A
- Authority
- CN
- China
- Prior art keywords
- agilawood
- mass
- parts
- culture medium
- yeast
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/83—Thymelaeaceae (Mezereum family), e.g. leatherwood or false ohelo
- A61K36/835—Aquilaria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Mycology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Forests & Forestry (AREA)
- Ecology (AREA)
- Biomedical Technology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Environmental Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Alternative & Traditional Medicine (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Medical Informatics (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to the field of agilawood manufacturing, and provides a preparation method of agilawood and agilawood for solving the problem of less effective ingredients in agilawood. The invention provides a preparation method of agilawood, which comprises the following steps: s11, mixing the amplified agarwood primary flora with yeast liquid and a solvent to obtain mixed bacterial liquid; s12, implanting the mixed bacterial liquid into an agilawood tree body; s13, after culturing for a period of time, harvesting agilawood. The primary flora, the yeast and the solvent are mixed in the aquilaria sinensis tree body, so that a large amount of natural agilawood with higher quality can be obtained in a short time.
Description
Technical Field
The invention relates to the field of agilawood manufacturing, and particularly relates to an agilawood and a preparation method thereof.
Background
The agilawood is a traditional and rare medicinal material in China, the Aquilaria plants (Aquilaria) can be induced to generate the agilawood by external stimulation, and the healthy Aquilaria sinensis cannot generate the agilawood by self, so that the natural agilawood resource is rare. The chemical components of the agilawood mainly comprise sesquiterpene, aromatic compounds and 2-phenethyl chromone compounds, wherein the sesquiterpene is the main drug effect component of the compounds, and pharmacological experiments prove that the compounds have obvious physiological activity on the nervous system. Although hundreds of thousands of mu of aquilaria sinensis trees which can produce the aquilaria sinensis are planted in China, and hundreds of thousands of mu are planted in countries in southeast Asia, the yield of the aquilaria sinensis is very low under natural conditions. Therefore, people summarize artificial methods for producing incense, such as a cutting injury method, a half-cutting method, a punching method, an iron nail method, an artificial inoculation method, a chemical method and the like, through scientific research and practical experience. However, the traditional manual agilawood formation method is complex to operate, the agilawood formation rate is low, the cost is high, and a great amount of waste is caused.
In the prior art, the method for inoculating the agilawood needs to be boiled, dissolved and sterilized, the above treatment mode is very complicated, and the agilawood production process is manufactured by using various working procedures. The aquilaria sinensis is produced by comprehensively infecting aquilaria sinensis with various floras after drilling, and the drilling treatment mode can cause certain damage to aquilaria sinensis trees, so that the service life of the aquilaria sinensis trees is shortened, the yield is low, the method is not in line with the long-term development strategy, and the green development policy of enterprises is violated.
How to develop a preparation method of agilawood with higher effective component and mild means is a technical problem to be solved urgently.
Disclosure of Invention
The invention solves the technical problem of less effective components in agilawood and provides a preparation method of agilawood.
In order to solve the technical problems, the technical scheme provided by the invention is as follows:
a method for preparing lignum Aquilariae Resinatum comprises:
s11, mixing the amplified agarwood primary flora with yeast liquid and a solvent to obtain mixed bacterial liquid;
s12, implanting the mixed bacterial liquid into an agilawood tree body;
s13, after culturing for a period of time, harvesting agilawood.
The native flora of lignum Aquilariae Resinatum is used for preparing lignum Aquilariae Resinatum, and the obtained lignum Aquilariae Resinatum has components closer to natural lignum Aquilariae Resinatum, and no other chemical substances are introduced.
The native flora, the yeast and the solvent are mixed in the aquilaria sinensis tree body, so that a large amount of natural agilawood can be obtained in a short time, and meanwhile, the agilawood has high quality.
Meanwhile, the tree body for producing the agilawood is not withered and rotten, the oil content of the produced agilawood is several times higher than that of the traditional agilawood formation mode, and the tree body can grow again and is continuously used for circularly producing the agilawood.
Preferably, the preparation method of the yeast bacterial liquid comprises the following steps: the yeast is cultured by adopting a Chinese herbal medicine culture medium, wherein the Chinese herbal medicine culture medium comprises one or more of eucommia ulmoides, rhizoma smilacis glabrae, medlar and cedar. The bacterial liquid prepared by the Chinese herbal medicine culture medium is used for promoting the production of the agilawood, so that the production efficiency can be remarkably improved, and the yield of the agilawood is improved.
Preferably, the Chinese herbal medicine culture medium comprises 10-20 parts by mass of eucommia ulmoides, 12-30 parts by mass of rhizoma smilacis glabrae, 10-25 parts by mass of medlar, 1-5 parts by mass of cedar wood, 10-20 parts by mass of brown sugar and 60-70 parts by mass of water. The Chinese herbal medicine culture medium is optimized, and the content of effective components in the agilawood product can be increased.
Preferably, the eucommia ulmoides is extracted and used as a raw material of a Chinese herbal medicine culture medium, and the preparation method of the eucommia ulmoides extract comprises the following steps: oven drying Eucommiae cortex, grinding into powder, adding 10 times of 60% ethanol, soaking at room temperature for 10min, swelling, heating to 60 deg.C, extracting for 30min, filtering, extracting the residue with 60% ethanol at 60 deg.C for 30min, and mixing the extractive solutions; passing the combined extractive solution through neutral alumina chromatographic column, eluting with 95% ethanol, and concentrating to obtain eluate; extracting the eluate with diethyl ether for 3 times, mixing extractive solutions, and evaporating to remove diethyl ether to obtain Eucommiae cortex extract.
Preferably, the medlar is used as a raw material of a Chinese herbal medicine culture medium after being extracted, and the preparation method of the medlar extract comprises the following steps: drying fructus Lycii at 80 deg.C, and grinding to obtain fructus Lycii powder; taking half of the fructus Lycii powder, adding 10 times of 70% ethanol, adjusting pH to 5 with citric acid, performing ultrasonic treatment at 40 deg.C for 10min, filtering, collecting filtrate, and removing solvent to obtain first powder; taking the other half of the medlar powder, adding petroleum ether with the amount of 10 times of the medlar powder, performing ultrasonic treatment at 30 ℃ for 5min, filtering, taking filtrate, and removing the solvent to obtain second powder; mixing the first powder and the second powder, dissolving with 10 times of ethanol, stirring in water bath at 50 deg.C for 20min, and filtering to obtain residue, i.e. fructus Lycii extract.
The bacterial liquid prepared by the culture medium prepared by the eucommia ulmoides extract and the medlar extract prepared by a certain method can effectively promote the formation of agilawood and improve the quality and the yield of agilawood.
Preferably, the Chinese herbal medicine culture medium comprises 15 parts by mass of eucommia ulmoides, 20 parts by mass of rhizoma smilacis glabrae, 15 parts by mass of medlar, 2 parts by mass of cedar, 13 parts by mass of brown sugar and 65 parts by mass of water.
Preferably, the method for implanting the mixed bacterial liquid into the aquilaria sinensis tree body comprises the following steps: injecting the bacterial liquid into the agilawood tree body in a pressurizing mode, wherein the pressurizing pressure is 4-10 MPa. The agilawood injection is injected into the aquilaria sinensis tree body in a pressurizing mode, so that the activity of the tree body can be preserved as much as possible, and the damage to the agilawood is reduced.
Preferably, the solvent is an aqueous solvent, and the aqueous solvent is mixed with the yeast liquid and the primary flora after being sterilized. The sterilized aqueous solvent can avoid introducing mixed bacteria.
Preferably, in the step S13, the pH inside the agarwood body is controlled to be 4 to 5 during the cultivation. The control of the pH value of the agilawood is a key means for ensuring the yield and the quality of the agilawood.
Preferably, in the step S13, the culture time is 8-12 months. A large amount of agilawood can be effectively obtained after 8-12 months of culture.
Preferably, the preparation method of the agilawood primary flora comprises the following steps: culturing an original ecological strain of natural agilawood in an agilawood culture medium, wherein the culture medium comprises 20-30 parts by mass of brown sugar and 70-80 parts by mass of water; inoculating the original strain into a culture medium, and culturing until the colony number is 1500000-.
The agilawood is prepared from 1-2% of agarol, 0.1-0.25% of benzyl acetone, 6-8% of aquilaria sinensis aldehyde, 10-12% of villous lactarius aldehyde, 10-13% of alkene and 8-10% of oleic acid.
Compared with the prior art, the invention has the beneficial effects that: the primary flora, the yeast and the solvent are mixed in the aquilaria sinensis tree body, so that a large amount of natural agilawood with higher quality can be obtained in a short time.
Meanwhile, the method utilizes natural agilawood (without any chemical and heavy metals); the effective component is higher than natural agilawood; the incense can be rapidly produced, and the incense production rate can reach more than 70 percent; the quantitative production of the agilawood becomes possible, and the uniform quality standard is favorably generated.
Detailed Description
The following examples are further illustrative of the present invention and are not intended to be limiting thereof.
Example 1
A method for preparing lignum Aquilariae Resinatum comprises:
s11, mixing the amplified agarwood primary flora with yeast liquid and a solvent to obtain mixed bacterial liquid; the preparation method of the yeast bacterial liquid comprises the following steps: culturing yeast by adopting a Chinese herbal medicine culture medium, wherein the Chinese herbal medicine culture medium comprises 15 parts by mass of eucommia ulmoides, 20 parts by mass of rhizoma smilacis glabrae, 15 parts by mass of medlar, 2 parts by mass of cedar wood, 13 parts by mass of brown sugar and 65 parts by mass of water; the solvent is an aqueous solvent, and the aqueous solvent is mixed with yeast liquid and a primary flora after being sterilized; the preparation method of the agilawood primary flora comprises the following steps: culturing an original ecological strain of natural agilawood in an agilawood culture medium, wherein the culture medium comprises 25 parts by mass of brown sugar and 75 parts by mass of water; inoculating the original ecological strain into a culture medium, and culturing until the colony number is 1500000-;
s12, implanting the mixed bacterial liquid into an agilawood tree body, and injecting the bacterial liquid into the agilawood tree body in a pressurizing mode, wherein the pressurizing pressure is 6 MPa. Pressurization can enable the bacterial liquid to be more effectively diffused to promote rapid fusion.
S13, controlling the pH value inside the agilawood tree body to be 4-4.2, and harvesting agilawood after culturing for 10 months.
The native flora of lignum Aquilariae Resinatum is used for preparing lignum Aquilariae Resinatum, and the obtained lignum Aquilariae Resinatum has components closer to natural lignum Aquilariae Resinatum, and no other substances are introduced. The primary flora, the yeast and the solvent are mixed in the aquilaria sinensis tree body, so that a large amount of natural agilawood with higher quality can be obtained in a short time. The bacterial liquid prepared by the Chinese herbal medicine culture medium is used for promoting the production of the agilawood, so that the production efficiency can be remarkably improved, and the yield of the agilawood is improved. The Chinese herbal medicine culture medium is optimized, and the content of effective components in the agilawood product can be increased. The agilawood injection is injected into the aquilaria sinensis tree body in a pressurizing mode, so that the activity of the tree body can be preserved as much as possible, and the damage to the agilawood is reduced. The sterilized aqueous solvent can avoid introducing mixed bacteria. The control of the pH value of the agilawood is a key means for ensuring the yield and the quality of the agilawood. A large amount of agilawood can be effectively obtained after 8-12 months of culture.
Meanwhile, the tree body for producing the agilawood is not withered and rotten, the oil content of the produced agilawood is several times higher than that of the traditional agilawood formation mode, and the tree body can grow again and is continuously used for circularly producing the agilawood.
Example 2
A method for preparing lignum Aquilariae Resinatum comprises:
s11, mixing the amplified agarwood primary flora with yeast liquid and a solvent to obtain mixed bacterial liquid; the preparation method of the yeast bacterial liquid comprises the following steps: culturing yeast by adopting a Chinese herbal medicine culture medium, wherein the Chinese herbal medicine culture medium comprises 10 parts by mass of eucommia ulmoides, 12 parts by mass of rhizoma smilacis glabrae, 10 parts by mass of medlar, 1 part by mass of cedar, 10 parts by mass of brown sugar and 65 parts by mass of water; the solvent is an aqueous solvent, and the aqueous solvent is mixed with yeast liquid and a primary flora after being sterilized; the preparation method of the agilawood primary flora comprises the following steps: culturing an original ecological strain of natural agilawood in an agilawood culture medium, wherein the culture medium comprises 20 parts by mass of brown sugar and 80 parts by mass of water; inoculating the original ecological strain to a culture medium, and culturing until the colony number is 1500000-2000000 to obtain an agilawood original flora;
s12, implanting the mixed bacterial liquid into an agilawood tree body, and injecting the bacterial liquid into the agilawood tree body in a pressurizing mode, wherein the pressurizing pressure is 4 MPa;
and S13, controlling the pH inside the agilawood tree body to be 4-5, and harvesting agilawood after culturing for 12 months.
Example 3
A method for preparing lignum Aquilariae Resinatum comprises:
s11, mixing the amplified agarwood primary flora with yeast liquid and a solvent to obtain mixed bacterial liquid; the preparation method of the yeast bacterial liquid comprises the following steps: culturing yeast by adopting a Chinese herbal medicine culture medium, wherein the Chinese herbal medicine culture medium comprises 20 parts by mass of eucommia ulmoides, 30 parts by mass of rhizoma smilacis glabrae, 25 parts by mass of Chinese wolfberry, 5 parts by mass of cedar wood, 20 parts by mass of brown sugar and 70 parts by mass of water; the solvent is an aqueous solvent, and the aqueous solvent is mixed with yeast liquid and a primary flora after being sterilized; the preparation method of the agilawood primary flora comprises the following steps: culturing an original ecological strain of natural agilawood in an agilawood culture medium, wherein the culture medium comprises 30 parts by mass of brown sugar and 70 parts by mass of water; inoculating the original ecological strain to a culture medium, and culturing until the colony number is 1500000-2000000 to obtain an agilawood original flora;
s12, implanting the mixed bacterial liquid into an agilawood tree body, and injecting the bacterial liquid into the agilawood tree body in a pressurizing mode, wherein the pressurizing pressure is 8 MPa;
and S13, controlling the pH inside the agilawood tree body to be 4-5, and harvesting agilawood after culturing for 10 months.
Example 4
A method for preparing lignum Aquilariae Resinatum comprises:
s11, mixing the amplified agarwood primary flora with yeast liquid and a solvent to obtain mixed bacterial liquid; the preparation method of the yeast bacterial liquid comprises the following steps: culturing yeast by adopting a Chinese herbal medicine culture medium, wherein the Chinese herbal medicine culture medium comprises 15 parts by mass of eucommia ulmoides, 20 parts by mass of rhizoma smilacis glabrae, 15 parts by mass of medlar, 2 parts by mass of cedar wood, 13 parts by mass of brown sugar and 65 parts by mass of water; the solvent is an aqueous solvent, and the aqueous solvent is mixed with yeast liquid and a primary flora after being sterilized; the preparation method of the agilawood primary flora comprises the following steps: culturing an original ecological strain of natural agilawood in an agilawood culture medium, wherein the culture medium comprises 25 parts by mass of brown sugar and 75 parts by mass of water; inoculating the original ecological strain to a culture medium, and culturing until the colony number is 1500000-2000000 to obtain an agilawood original flora;
s12, implanting the mixed bacterial liquid into an agilawood tree body, and injecting the bacterial liquid into the agilawood tree body in a pressurizing mode, wherein the pressurizing pressure is 6 MPa;
and S13, controlling the pH inside the agilawood tree body to be 4-4.2, and harvesting agilawood after culturing for 10 months.
The eucommia ulmoides is extracted and used as a raw material of a Chinese herbal medicine culture medium, and the preparation method of the eucommia ulmoides extract comprises the following steps: oven drying Eucommiae cortex, grinding into powder, adding 10 times of 60% ethanol, soaking at room temperature for 10min, swelling, heating to 60 deg.C, extracting for 30min, filtering, extracting the residue with 60% ethanol at 60 deg.C for 30min, and mixing the extractive solutions; passing the combined extractive solution through neutral alumina chromatographic column, eluting with 95% ethanol, and concentrating to obtain eluate; extracting the eluate with diethyl ether for 3 times, mixing extractive solutions, and evaporating to remove diethyl ether to obtain Eucommiae cortex extract.
The medlar is extracted and then used as a raw material of a Chinese herbal medicine culture medium, and the preparation method of the medlar extract comprises the following steps: drying fructus Lycii at 80 deg.C, and grinding to obtain fructus Lycii powder; taking half of the fructus Lycii powder, adding 10 times of 70% ethanol, adjusting pH to 5 with citric acid, performing ultrasonic treatment at 40 deg.C for 10min, filtering, collecting filtrate, and removing solvent to obtain first powder; taking the other half of the medlar powder, adding petroleum ether with the amount of 10 times of the medlar powder, performing ultrasonic treatment at 30 ℃ for 5min, filtering, taking filtrate, and removing the solvent to obtain second powder; mixing the first powder and the second powder, dissolving with 10 times of ethanol, stirring in water bath at 50 deg.C for 20min, and filtering to obtain residue, i.e. fructus Lycii extract.
The bacterial liquid prepared by the culture medium prepared by the eucommia ulmoides extract and the medlar extract prepared by a certain method can effectively promote the formation of agilawood and improve the quality and the yield of agilawood.
Example 5
A method for preparing lignum Aquilariae Resinatum comprises:
s11, mixing the amplified agarwood primary flora with yeast liquid and a solvent to obtain mixed bacterial liquid; the preparation method of the yeast bacterial liquid comprises the following steps: culturing saccharomycetes by adopting a Chinese herbal medicine culture medium, wherein the Chinese herbal medicine culture medium comprises 20 parts by mass of glabrous greenbrier rhizome, 15 parts by mass of medlar, 2 parts by mass of cedar, 13 parts by mass of brown sugar and 80 parts by mass of water; the solvent is an aqueous solvent, and the aqueous solvent is mixed with yeast liquid and a primary flora after being sterilized; the preparation method of the agilawood primary flora comprises the following steps: culturing an original ecological strain of natural agilawood in an agilawood culture medium, wherein the culture medium comprises 25 parts by mass of brown sugar and 75 parts by mass of water; inoculating the original ecological strain to a culture medium, and culturing until the colony number is 1500000-2000000 to obtain an agilawood original flora;
s12, implanting the mixed bacterial liquid into an agilawood tree body, and injecting the bacterial liquid into the agilawood tree body in a pressurizing mode, wherein the pressurizing pressure is 6 MPa;
and S13, controlling the pH inside the agilawood tree body to be 4-4.2, and harvesting agilawood after culturing for 10 months.
Example 6
A method for preparing lignum Aquilariae Resinatum comprises:
s11, mixing the amplified agarwood primary flora with yeast liquid and a solvent to obtain mixed bacterial liquid; the preparation method of the yeast bacterial liquid comprises the following steps: culturing yeast by adopting a Chinese herbal medicine culture medium, wherein the Chinese herbal medicine culture medium comprises 15 parts by mass of eucommia ulmoides, 15 parts by mass of medlar, 2 parts by mass of cedar, 13 parts by mass of brown sugar and 85 parts by mass of water; the solvent is an aqueous solvent, and the aqueous solvent is mixed with yeast liquid and a primary flora after being sterilized; the preparation method of the agilawood primary flora comprises the following steps: culturing an original ecological strain of natural agilawood in an agilawood culture medium, wherein the culture medium comprises 25 parts by mass of brown sugar and 75 parts by mass of water; inoculating the original ecological strain to a culture medium, and culturing until the colony number is 500-3000 to obtain an agilawood original flora;
s12, implanting the mixed bacterial liquid into an agilawood tree body, and injecting the bacterial liquid into the agilawood tree body in a pressurizing mode, wherein the pressurizing pressure is 6 MPa;
and S13, controlling the pH inside the agilawood tree body to be 4-4.2, and harvesting agilawood after culturing for 10 months.
Example 7
A method for preparing lignum Aquilariae Resinatum comprises:
s11, mixing the amplified agarwood primary flora with yeast liquid and a solvent to obtain mixed bacterial liquid; the preparation method of the yeast bacterial liquid comprises the following steps: culturing yeast by adopting a Chinese herbal medicine culture medium, wherein the Chinese herbal medicine culture medium comprises 15 parts by mass of eucommia ulmoides, 20 parts by mass of rhizoma smilacis glabrae, 2 parts by mass of cedar, 13 parts by mass of brown sugar and 80 parts by mass of water; the solvent is an aqueous solvent, and the aqueous solvent is mixed with yeast liquid and a primary flora after being sterilized; the preparation method of the agilawood primary flora comprises the following steps: culturing an original ecological strain of natural agilawood in an agilawood culture medium, wherein the culture medium comprises 25 parts by mass of brown sugar and 75 parts by mass of water; inoculating the original ecological strain to a culture medium, and culturing until the colony number is 500-3000 to obtain an agilawood original flora;
s12, implanting the mixed bacterial liquid into an agilawood tree body, and injecting the bacterial liquid into the agilawood tree body in a pressurizing mode, wherein the pressurizing pressure is 6 MPa;
and S13, controlling the pH inside the agilawood tree body to be 4-4.2, and harvesting agilawood after culturing for 10 months.
Example 8
A method for preparing lignum Aquilariae Resinatum comprises:
s11, mixing the amplified agarwood primary flora with yeast liquid and a solvent to obtain mixed bacterial liquid; the preparation method of the yeast bacterial liquid comprises the following steps: culturing yeast by adopting a Chinese herbal medicine culture medium, wherein the Chinese herbal medicine culture medium comprises 15 parts by mass of eucommia ulmoides, 20 parts by mass of rhizoma smilacis glabrae, 15 parts by mass of medlar, 13 parts by mass of brown sugar and 67 parts by mass of water; the solvent is an aqueous solvent, and the aqueous solvent is mixed with yeast liquid and a primary flora after being sterilized; the preparation method of the agilawood primary flora comprises the following steps: culturing an original ecological strain of natural agilawood in an agilawood culture medium, wherein the culture medium comprises 25 parts by mass of brown sugar and 75 parts by mass of water; inoculating the original ecological strain to a culture medium, and culturing until the colony number is 1500000-2000000 to obtain an agilawood original flora;
s12, implanting the mixed bacterial liquid into an agilawood tree body, and injecting the bacterial liquid into the agilawood tree body in a pressurizing mode, wherein the pressurizing pressure is 6 MPa;
and S13, controlling the pH inside the agilawood tree body to be 4-4.2, and harvesting agilawood after culturing for 10 months.
Comparative example 1
A method for preparing lignum Aquilariae Resinatum comprises:
s11, mixing the amplified agarwood primary flora with yeast liquid and a solvent to obtain mixed bacterial liquid; the preparation method of the yeast bacterial liquid comprises the following steps: adopting a culture medium to culture the yeast, wherein the culture medium comprises 13 parts by mass of brown sugar and 65 parts by mass of water; the solvent is an aqueous solvent, and the aqueous solvent is mixed with yeast liquid and a primary flora after being sterilized; the preparation method of the agilawood primary flora comprises the following steps: culturing an original ecological strain of natural agilawood in an agilawood culture medium, wherein the culture medium comprises 25 parts by mass of brown sugar and 75 parts by mass of water; inoculating the original ecological strain to a culture medium, and culturing until the colony number is 1500000-2000000 to obtain an agilawood original flora;
s12, implanting the mixed bacterial liquid into an agilawood tree body, and injecting the bacterial liquid into the agilawood tree body in a pressurizing mode, wherein the pressurizing pressure is 6 MPa;
and S13, controlling the pH inside the agilawood tree body to be 4-4.2, and harvesting agilawood after culturing for 10 months.
Examples of the experiments
Agilawood is prepared according to the preparation methods of examples 1-8 and comparative example 1, and the content of active ingredients of the agilawood is detected by a GC/MS area normalization method, which is shown in the following table.
TABLE 1 effective substance contents of lignum Aquilariae Resinatum produced by the methods of examples 1-8 and comparative example 1
| Linalool | Benzyl acetone | Aquilaria sinensis aldehyde | Lactarius velutina aldehyde | Alkenes | Oleic acid | |
| Example 1 | 1.26% | 0.23% | 6.25% | 10.55% | 10.66% | 8.15% |
| Example 2 | 1.13% | 0.21% | 5.68% | 10.41% | 10.26% | 8.01% |
| Example 3 | 1.06% | 0.22% | 6.16% | 10.23% | 10.55% | 8.11% |
| Example 4 | 1.97% | 0.15% | 7.68% | 11.56% | 12.56% | 9.55% |
| Example 5 | 0.35% | 0.54% | 1.22% | 5.95% | 4.59% | 1.66% |
| Example 6 | 0.45% | 0.53% | 1.68% | 4.23% | 4.26% | 2.17% |
| Example 7 | 0.47% | 0.33% | 2.02% | 5.16% | 5.87% | 2.56% |
| Example 8 | 0.56% | 0.66% | 2.51% | 4.87% | 5.36% | 2.12% |
| Comparative example 1 | 0.33% | 0.77% | 0.96% | 1.52% | 3.77% | 0.89% |
As can be seen from table 1, the yield of agilawood can be increased only by using specific traditional Chinese medicines as raw materials of the yeast culture medium, the traditional Chinese medicine culture mediums in examples 5 to 8 are all combinations of 3 traditional Chinese medicines, but the content of active ingredients of the traditional Chinese medicines is obviously lower than that of the traditional Chinese medicines in examples 1 to 4, which indicates that none of the 4 traditional Chinese medicines is available. The comparative example 1 does not contain traditional Chinese medicines, the content of active ingredients is lower, and the content of active ingredients in agilawood is lower.
In the embodiment 4, the extraction method of eucommia ulmoides and medlar is optimized, and the content of the effective components of agilawood is further improved on the basis of the embodiments 1-3.
As is known, the lignum Aquilariae Resinatum tree can be damaged to produce incense, and all previous research units surround the research in the manner, so that the lignum Aquilariae Resinatum tree is damaged to an indefinite extent, and even a part of the lignum Aquilariae Resinatum tree is withered. The invention applies unique reverse thinking and adopts the technology of inducing fusion, and the bacteria liquid is pressed into the tree body without damaging the tree body, so that more components can be secreted out of the agilawood tree body.
The above detailed description is specific to possible embodiments of the present invention, and the above embodiments are not intended to limit the scope of the present invention, and all equivalent implementations or modifications that do not depart from the scope of the present invention should be included in the present claims.
Claims (10)
1. The preparation method of agilawood is characterized by comprising the following steps:
s11, mixing the amplified agarwood primary flora with yeast liquid and a solvent to obtain mixed bacterial liquid;
s12, implanting the mixed bacterial liquid into an agilawood tree body;
s13, after culturing for a period of time, harvesting agilawood.
2. The method for preparing agilawood according to claim 1, wherein the method for preparing the yeast bacterial liquid comprises the following steps: the yeast is cultured by adopting a Chinese herbal medicine culture medium, wherein the Chinese herbal medicine culture medium comprises one or more of eucommia ulmoides, rhizoma smilacis glabrae, medlar and cedar.
3. The method for preparing agilawood according to claim 2, wherein the Chinese herbal medicine culture medium comprises 10-20 parts by mass of eucommia ulmoides, 12-30 parts by mass of rhizoma smilacis glabrae, 10-25 parts by mass of medlar, 1-5 parts by mass of cedar wood, 10-20 parts by mass of brown sugar and 60-70 parts by mass of water.
4. The method for preparing agilawood according to claim 1, wherein the Chinese herbal medicine culture medium comprises 15 parts by mass of eucommia ulmoides, 20 parts by mass of rhizoma smilacis glabrae, 15 parts by mass of medlar, 2 parts by mass of cedar wood, 13 parts by mass of brown sugar and 65 parts by mass of water.
5. The method for preparing agilawood according to claim 1, wherein the method for implanting the mixed bacteria liquid into aquilaria sinensis trees comprises the following steps: injecting the bacterial liquid into the agilawood tree body in a pressurizing mode, wherein the pressurizing pressure is 4-10 MPa.
6. The method for preparing agilawood according to claim 1, wherein the solvent is an aqueous solvent, and the aqueous solvent is mixed with the yeast liquid and the primary flora after being sterilized.
7. The method for preparing agilawood according to claim 1, wherein in the step S13, the pH inside the agilawood tree body is controlled to be 4-5 in the culture process.
8. The method for preparing agilawood according to claim 1, wherein in the step of S13, the culture time is 8-12 months.
9. The method for preparing agilawood according to claim 1, wherein the method for preparing agilawood primary flora comprises the following steps: culturing an original ecological strain of natural agilawood in an agilawood culture medium, wherein the culture medium comprises 20-30 parts by mass of brown sugar and 70-80 parts by mass of water; inoculating the original ecological strain into a culture medium, and culturing until the colony number is 1500000-2000000 to obtain the agilawood original flora.
10. The preparation method of agilawood is characterized by comprising 1-2% of agarol, 0.1-0.25% of benzyl acetone, 6-8% of aquilaria aldehyde, 10-12% of villous lactarius aldehyde, 10-13% of alkenes and 8-10% of oleic acid according to any one of claims 1-9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910918996.7A CN110876310A (en) | 2019-09-26 | 2019-09-26 | Agilawood preparation method and agilawood |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910918996.7A CN110876310A (en) | 2019-09-26 | 2019-09-26 | Agilawood preparation method and agilawood |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110876310A true CN110876310A (en) | 2020-03-13 |
Family
ID=69727796
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910918996.7A Pending CN110876310A (en) | 2019-09-26 | 2019-09-26 | Agilawood preparation method and agilawood |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110876310A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111713328A (en) * | 2020-07-07 | 2020-09-29 | 刘志强 | Technology for planting agilawood fungi into daphneceae trees for forming incense |
| CN115779019A (en) * | 2022-06-20 | 2023-03-14 | 海南臻香农业技术开发有限公司 | Method for planting plant stem cells to generate agilawood |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105830765A (en) * | 2016-04-26 | 2016-08-10 | 唐文君 | Method for generating fragrance of agilawood through natural fungi |
| CN107624414A (en) * | 2017-09-15 | 2018-01-26 | 广东森呼吸生物科技有限公司 | A kind of natural agilawood Edgeworthia chrysantha method |
| CN108307849A (en) * | 2017-01-17 | 2018-07-24 | 昱达国际生化科技有限公司 | Promote the method for agallochum secretion agalloch eaglewood resin |
| TW201831076A (en) * | 2017-02-24 | 2018-09-01 | 高誌懇 | A method of forming the agilawood oil and tissue made by using the same |
-
2019
- 2019-09-26 CN CN201910918996.7A patent/CN110876310A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105830765A (en) * | 2016-04-26 | 2016-08-10 | 唐文君 | Method for generating fragrance of agilawood through natural fungi |
| CN108307849A (en) * | 2017-01-17 | 2018-07-24 | 昱达国际生化科技有限公司 | Promote the method for agallochum secretion agalloch eaglewood resin |
| TW201831076A (en) * | 2017-02-24 | 2018-09-01 | 高誌懇 | A method of forming the agilawood oil and tissue made by using the same |
| CN107624414A (en) * | 2017-09-15 | 2018-01-26 | 广东森呼吸生物科技有限公司 | A kind of natural agilawood Edgeworthia chrysantha method |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111713328A (en) * | 2020-07-07 | 2020-09-29 | 刘志强 | Technology for planting agilawood fungi into daphneceae trees for forming incense |
| CN115779019A (en) * | 2022-06-20 | 2023-03-14 | 海南臻香农业技术开发有限公司 | Method for planting plant stem cells to generate agilawood |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104784230B (en) | The preparation method of fermented red ginseng and fermented red ginseng | |
| CN104230405A (en) | Method for preparing active algae extract foliar fertilizer | |
| CN103989715B (en) | Series of products and method are prepared using pine needle | |
| CN110876310A (en) | Agilawood preparation method and agilawood | |
| CN110734809A (en) | extraction method of agilawood essential oil | |
| CN104650251A (en) | Extraction method of mythic fungus multi-sugar alcohol | |
| CN105399521A (en) | Organic fertilizer for capsicum and preparation method thereof | |
| CN111286406A (en) | Extraction method of eucalyptus essential oil, eucalyptus essential oil and application | |
| CN106614820A (en) | Composition for preventing and treating anthracnose of spinach | |
| CN109054992A (en) | A method of extracting Seabuckthorm Seed Oil | |
| CN105494461B (en) | A kind of reagent for preventing and treating apple tree canker | |
| CN104547286A (en) | Method for preparing Chinese Eaglewood leaf extract | |
| CN104593199B (en) | A kind of method utilizing Radix Salviae Miltiorrhizae decoction dregs brewing healthy yellow rice wine | |
| CN106316624A (en) | Management Method of controlling apple tree canker | |
| CN103360611A (en) | Method for extracting gutta-percha from folium cortex eucommiae, skin or fruit | |
| CN111996223A (en) | Method for obtaining biological polysaccharide of lucid ganoderma and isatis root by utilizing bidirectional fermentation technology | |
| CN104403798B (en) | Preparation method of eucommia ulmoides mixed essential oil | |
| CN104383111A (en) | Kudzu plant fermentation broth and preparation method thereof | |
| CN107027815A (en) | A kind of method that Phytolacca acinosa that ferments prepares insecticide | |
| CN109055085B (en) | Rhizoma polygonati wine and preparation method thereof | |
| CN108420058B (en) | Cactus and ginkgo enzyme and preparation method thereof | |
| CN111991479A (en) | Bidirectional solid fermentation method of radix isatidis and folium isatidis dregs | |
| CN104403797B (en) | Preparation method of eucommia ulmoides essential oil | |
| CN104403796B (en) | Method for extracting essential oil from eucommia ulmoides | |
| CN107467665A (en) | A kind of method for the extraction olive growing leaves bioactive substance that ferments |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200313 |
|
| RJ01 | Rejection of invention patent application after publication |