CN110863048A - Probe library, detection method and kit for detecting effectiveness of DNA homologous recombination repair pathway - Google Patents

Probe library, detection method and kit for detecting effectiveness of DNA homologous recombination repair pathway Download PDF

Info

Publication number
CN110863048A
CN110863048A CN201911239803.1A CN201911239803A CN110863048A CN 110863048 A CN110863048 A CN 110863048A CN 201911239803 A CN201911239803 A CN 201911239803A CN 110863048 A CN110863048 A CN 110863048A
Authority
CN
China
Prior art keywords
dna
gene
seq
sequence corresponding
probe sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201911239803.1A
Other languages
Chinese (zh)
Inventor
刘松柏
王晓晓
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Suzhou Vocational Health College
Original Assignee
Suzhou Vocational Health College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Suzhou Vocational Health College filed Critical Suzhou Vocational Health College
Priority to CN201911239803.1A priority Critical patent/CN110863048A/en
Publication of CN110863048A publication Critical patent/CN110863048A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/118—Prognosis of disease development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)

Abstract

The invention provides a probe library, a detection method and a kit for detecting the effectiveness of a DNA homologous recombination repair pathway, wherein the probe library consists of probes shown in SEQ ID NO. 1-579, and the probe library is prepared into the detection kit which can be used for detecting genes related to the DNA homologous recombination repair pathway. The probe library is used for enriching target DNA fragments to be analyzed, and then sequencing is carried out on the enriched DNA fragments through a sequencing means, so that the effectiveness of gene detection is realized. The gene related to the DNA homologous recombination repair pathway can be used for predicting the occurrence tendency of tumors, evaluating the malignancy degree and clinical prognosis of the tumors and guiding the screening and research and development of new drugs, so the invention has important guiding significance on the research direction of gene functionality.

Description

Probe library, detection method and kit for detecting effectiveness of DNA homologous recombination repair pathway
Technical Field
The invention belongs to the technical field of gene detection, and particularly relates to a probe library, a detection method and a kit for detecting the effectiveness of a DNA homologous recombination repair pathway.
Background
The abnormal DNA damage repair pathway is closely related to the tumor generation tendency and the sensitivity of the DNA damage inducing anti-tumor drugs to cells. The DNA homologous recombination repair pathway mainly repairs DNA damage of which single-strand damage repair fails when cells are in S phase and G2 phase, DNA damage caused by topoisomerase inhibitors, and DNA double-strand crosslinking damage caused by alkylating agents such as TMZ and the like, and is one of the most important ways in a DNA repair mechanism. In brief, when the DNA is damaged by double strand break at S phase and G2 phase of the cell, ATM/ATR is used as a damage sensing element to phosphorylate H2A, then MRE11/RAD50/NBS1 complex (MRN) MRE11 cuts the broken end of the DNA in the direction of 5 '-3' end by using nuclease activity, and the exposed single strand DNA is blocked and protected by RPA. The RAD51 protein complex then replaces the RPA and searches for homologous DNA templates, and invades the 3' end into the template strand with the help of proteins such as RAD54 and forms a D-loop structure. The process of DNA damage repair is then completed by the final formation of two complete DNA strands through the steps of DNA synthesis, shearing, and re-stitching with the aid of a template.
Synthetic Lethality (Synthetic Lethality) strategy has been widely used in recent years in the study of tumors. The concept of synthetic lethality in tumor studies is: when the function of two important genes that are not in the same repair pathway is suppressed from one of them, the cell is not lethal, but after the function of both genes is suppressed, the cell is dead. In clinic, if one of the mutations exists in the tumor cells, and the gene is normal in normal cells, the other gene can be used as a target point, and the function of the other gene is inhibited, so that the aims of specifically killing the tumor cells and individually treating the tumor cells are fulfilled.
Earlier researches find that the gene involved in the DNA homologous recombination repair pathway has embryonic genetic mutation and somatic mutation in specific tissues, and the genetic mutation and somatic mutation are highly related to the generation and treatment effects of tumors. Wherein, BRCA1/BRCA2 gene mutation will increase the probability of suffering from breast cancer, ovarian cancer, melanoma and hematologic tumor; XRCC2 gene mutation tumor cells are more sensitive to cross-linked chemotherapeutic drugs; the rate of frameshift mutations in the RAD50 gene is up to 30% in gastrointestinal cancers. The mutation of the genes causes the change (attenuation or deletion) of the effectiveness of the DNA homologous recombination repair pathway, and has important significance for the generation, prognosis and treatment strategy formulation of tumor diseases.
Disclosure of Invention
The invention aims to provide a probe library, a detection method and a kit for detecting the effectiveness of a DNA homologous recombination repair pathway.
In order to achieve the purpose, the invention provides the following technical scheme:
a kit for detecting the effectiveness of a DNA homologous recombination repair pathway comprises a probe library which is composed of probes shown in SEQ ID NO. 1-579 and aims at genes related to the DNA homologous recombination repair pathway.
Further, the genes related to the DNA homologous recombination repair pathway include LIG1, MRE11, NBN, PARG, PARP1, PARPBP, RAD50, TP53BP1, XRCC2, XRCC3, EXO1, PCNA, POLD1, POLD2, POLD3, POLD4, RFC1, RFC2, RFC3, RFC4, RFC5, RPA1, RPA2, RPA3, RPA RAD 4, BARD1, BLM, BRCA1, BRCA2, BRIP 2, DMC EI 2, DNA2, RFD 2, EME2, ERCC 2, FACCM 2, FEN 2, GEN 2, H2, HELQ, HFM 2, INO 2, KANST 2, MUT 2, SMNSS 2, PSNSS 2, PSNS7, PSNSRADR 2, PSRQRADR 2, PSRWD 2, PSR3672, PSRWD 2, PSR3672, PSRNS7, PSR3672, PSRNS3672, PSR36.
Further, probe sequences corresponding to the gene LIG1 are shown as SEQ ID NO. 1-11, probe sequences corresponding to the gene MRE11 are shown as SEQ ID NO. 12-17, probe sequences corresponding to the gene NBN are shown as SEQ ID NO. 18-23, probe sequences corresponding to the gene PARG are shown as SEQ ID NO. 24-29, probe sequences corresponding to the gene PARP1 are shown as SEQ ID NO. 30-40, probe sequences corresponding to the gene PARPBP are shown as SEQ ID NO. 41-45, probe sequences corresponding to the gene RAD50 are shown as SEQ ID NO. 46-57, probe sequences corresponding to the gene TP53BP1 are shown as SEQ ID NO. 58-71, probe sequences corresponding to the gene XRCC2 are shown as SEQ ID NO. 72-74, probe sequences corresponding to the gene XRCC3 are shown as SEQ ID NO. 75-78, probe sequences corresponding to the gene EXO1 are shown as SEQ ID NO. 84, and probe sequences corresponding to SEQ ID NO. 88-79, the probe sequence corresponding to the gene POLD1 is shown as SEQ ID NO. 89-101, the probe sequence corresponding to the gene POLD2 is shown as SEQ ID NO. 102-106, the probe sequence corresponding to the gene POLD3 is shown as SEQ ID NO. 107-110, the probe sequence corresponding to the gene POLD4 is shown as SEQ ID NO. 111-113, the probe sequence corresponding to the gene RFC1 is shown as SEQ ID NO. 114-123, the probe sequence corresponding to the gene RFC2 is shown as SEQ ID NO. 124-127, the probe sequence corresponding to the gene RFC3 is shown as SEQ ID NO. 128-131, the probe sequence corresponding to the gene RFC4 is shown as SEQ ID NO. 132-135, the probe sequence corresponding to the gene RFC5 is shown as SEQ ID NO. 136-138, the probe sequence corresponding to the gene RPA1 is shown as SEQ ID NO. 139-144, the probe sequence corresponding to the gene RPA2 is shown as SEQ ID NO. 145-148, and the probe sequence corresponding to SEQ ID NO. 151-3, the probe sequence corresponding to the gene RPA4 is shown as SEQ ID NO. 152-153, the probe sequence corresponding to the gene BARD1 is shown as SEQ ID NO. 154-158, the probe sequence corresponding to the gene BLM is shown as SEQ ID NO. 159-169, the probe sequence corresponding to the gene BRCA1 is shown as SEQ ID NO. 170-178, the probe sequence corresponding to the gene BRCA2 is shown as SEQ ID NO. 179-194, the probe sequence corresponding to the gene BRIP1 is shown as SEQ ID NO. 195-204, the probe sequence corresponding to the gene EME1 is shown as SEQ ID NO. 205-209, the probe sequence corresponding to the gene DNA2 is shown as SEQ ID NO. 210-219, the probe sequence corresponding to the gene EID3 is shown as SEQ ID NO. 220-221, the probe sequence corresponding to the gene EME1 is shown as SEQ ID NO. 222-225, the probe sequence corresponding to the gene EME2 is shown as SEQ ID NO. 229, and the probe sequence corresponding to ERCC NO. 230-1, the probe sequence corresponding to the gene FANCM is shown as SEQ ID NO. 233-245, the probe sequence corresponding to the gene FEN1 is shown as SEQ ID NO. 246-248, the probe sequence corresponding to the gene GEN1 is shown as SEQ ID NO. 249-254, the probe sequence corresponding to the gene H2AFX is shown as SEQ ID NO. 255-256, the probe sequence corresponding to the gene HELQ is shown as SEQ ID NO. 257-265, the probe sequence corresponding to the gene HFM1 is shown as SEQ ID NO. 266-280, the probe sequence corresponding to the gene INO80 is shown as SEQ ID NO. 281-294, the probe sequence corresponding to the gene KAT5 is shown as SEQ ID NO. 295-300, the probe sequence corresponding to the gene MUS81 is shown as SEQ ID NO. 301-307, the probe sequence corresponding to the gene MCNFATC 2IP is shown as SEQ ID NO. 308-310, the probe sequence corresponding to the gene NSE 1 is shown as SEQ ID NO. 318-315, the probe sequence corresponding to NSE NO.2 is shown as SEQ ID NO. 316-46316, the probe sequence corresponding to the gene NSMCE3 is shown as SEQ ID NO. 319-320, the probe sequence corresponding to the gene NSMCE4A is shown as SEQ ID NO. 321-324, the probe sequence corresponding to the gene PALB2 is shown as SEQ ID NO. 325-331, the probe sequence corresponding to the gene PARP2 is shown as SEQ ID NO. 332-337, the probe sequence corresponding to the gene PAXIP1 is shown as SEQ ID NO. 338-346, the probe sequence corresponding to the gene POLH is shown as SEQ ID NO. 347-351, the probe sequence corresponding to the gene POLQ is shown as SEQ ID NO. 352-369, the probe sequence corresponding to the gene PPP4C is shown as SEQ ID NO. 370-372, the probe sequence corresponding to the gene PPP4R1 is shown as SEQ ID NO. 373-380, the probe sequence corresponding to the gene PPP4R2 is shown as SEQ ID NO. 381-384, the probe sequence corresponding to the gene SEQ ID NO. 35385-398, the probe sequence corresponding to the gene PPP NO.373 is shown as SEQ ID NO. 26-395, the probe sequence corresponding to the gene RAD51B is shown as SEQ ID NO. 399-402, the probe sequence corresponding to the gene RAD51C is shown as SEQ ID NO. 403-405, the probe sequence corresponding to the gene RAD51D is shown as SEQ ID number 406-409, the probe sequence corresponding to the gene RAD52 is shown as SEQ ID number 410-413, the probe sequence corresponding to the gene RAD54B is shown as SEQ ID number 414-419, the probe sequence corresponding to the gene RAD54L is shown as SEQ ID NO. 420-426, the probe sequence corresponding to the gene RBBP8 is shown as SEQ ID NO. 427-434, the probe sequence corresponding to the gene RDM1 is shown as SEQ ID number 435-439, the probe sequence corresponding to the gene RECQL is shown as SEQ ID NO. 440-445, the probe sequence corresponding to the gene RECQL4 is shown as SEQ ID number 446-402, the probe sequence corresponding to gene RECQL5 is shown as SEQ ID NO. 457-464, the probe sequence corresponding to the gene RMI1 is shown as SEQ ID number 465-468, the probe sequence corresponding to the gene RMI2 is shown as SEQ ID number 469-470, the probe sequence corresponding to the gene RTEL1 is shown as SEQ ID No. 471-483, the probe sequence corresponding to the gene SHFM1 is shown as SEQ ID No.484, the probe sequence corresponding to the gene SLX1A is shown as SEQ ID No. 485-489, the probe sequence corresponding to the gene SLX1B is shown as SEQ ID number 490-494, the probe sequence corresponding to the gene SLX4 is shown as SEQ ID No. 495-503, the probe sequence corresponding to the gene SMARCAD1 is shown as SEQ ID number-513, the probe sequence corresponding to the gene SMC5 is shown as SEQ ID number 514-524, the probe sequence corresponding to the gene SMC6 is shown as SEQ ID number 536, and the probe sequence corresponding to the gene SPO11 is shown as SEQ ID No. 537-540, the probe sequence corresponding to the gene SWSAP1 is shown as SEQ ID number 541-542, the probe sequence corresponding to the gene TOP3A is shown as SEQ ID number 543-550, the probe sequence corresponding to the gene TOP3B is shown as SEQ ID number 551-557, the probe sequence corresponding to the gene UIMC1 is shown as SEQ ID number 558-562, the probe sequence corresponding to the gene WRN is shown as SEQ ID number 563-575, and the probe sequence corresponding to the gene ZWIM 7 is shown as SEQ ID number 576-579.
A method for detecting a gene associated with a DNA homologous recombination repair pathway comprising the steps of:
(1) obtaining a DNA sample library of a subject;
(2) hybridizing all probes shown as SEQ ID NO. 1-579 in the kit of claim 1 with the DNA sample library;
(3) and (3) separating the hybridization product in the step (2), releasing the gene segment hybridized with the probe in the kit, and sequencing the separated gene segment by adopting a sequencing technology so as to detect the mutation condition of the gene.
Further, the DNA sample library in the step (1) is composed of double-stranded DNA fragments;
the step (1) comprises the following steps: extracting whole genome DNA, and then fragmenting the whole genome DNA; or mRNA is extracted, fragmented, and then double-stranded cDNA is synthesized using the fragmented mRNA as a template.
Further, the probe in the step (2) is selectively labeled.
Further, the probe in the step (2) is labeled with biotin.
Has the advantages that: the invention discloses a probe library, a detection method and a kit for detecting the effectiveness of a DNA homologous recombination repair pathway, which are used for integrally detecting and analyzing the variation condition of the DNA homologous recombination repair pathway participating in genes and mainly have the following meanings:
1. by analyzing the variation condition of the DNA homologous recombination repair pathway participating in the gene, the effectiveness (attenuation or deletion) of the DNA homologous recombination repair pathway in human tissues or cells is evaluated, the occurrence tendency of tumor diseases is predicted, and patients are guided to take corresponding preventive measures.
2. The effectiveness (weakening or deletion) of the DNA homologous recombination repair pathway in the cells of the tumor patients is evaluated by analyzing the variation condition of the DNA homologous recombination repair pathway participating in the gene, and the method has important guiding significance for evaluating the malignancy degree and clinical prognosis of the tumor.
3. The effectiveness (attenuation or deletion) of the DNA homologous recombination repair pathway in the cells of the tumor patients is evaluated by analyzing the variation condition of the DNA homologous recombination repair pathway participating in the gene, and the method has important guiding significance for the selection of radiation and chemotherapy medicaments and the formulation of treatment strategies (such as synthetic lethal scheme and the like).
4. The effectiveness (weakening or deletion) of the DNA homologous recombination repair pathway in the cells of the tumor patients is evaluated by analyzing the variation condition of the DNA homologous recombination repair pathway participating in the gene, and the method has important guiding significance for the selection of new therapeutic targets, the research and development of targeted drugs and the like.
Drawings
FIG. 1 is a flow chart of the detection method of the present invention.
Detailed Description
The present invention is further described below with reference to specific examples, which are only exemplary and do not limit the scope of the present invention in any way. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.
The invention provides a method for repairing a gene related to a pathway by DNA base excision, which mainly comprises the following steps:
(1) obtaining a DNA sample library of a subject; extracting whole genome DNA, and then fragmenting the whole genome DNA; or extracting mRNA, fragmenting the mRNA, and synthesizing double-stranded cDNA by using the fragmented mRNA as a template;
(2) designing a DNA probe hybridized with the gene aiming at the gene segment to be detected, and screening a plurality of probes from the DNA probe library as a DNA probe library;
(3) hybridizing the DNA probe library with the DNA sample library, and enriching the DNA sample library to obtain a DNA fragment of the gene to be detected;
(4) and (4) separating the hybridization product in the step (3), releasing the gene segment hybridized with the probe in the kit, and sequencing the separated gene segment by adopting a sequencing technology so as to detect the mutation condition of the gene.
In the implementation process, each probe in the DNA probe library may be labeled with biotin, and then the magnetic beads labeled with streptavidin are used to adsorb the hybridization product after hybridization, releasing the enriched gene fragment to be detected from the magnetic beads, and then sequencing the gene fragment.
The specific experimental process is as follows:
first, prepare cDNA/DNA sample library
1. Preparation of DNA sample library
1.1 DNA extraction
Human genome DNA is extracted by a commercial DNA extraction kit, and the quality and the concentration of a DNA template are detected by a spectrophotometry and a gel electrophoresis system for the extracted DNA. The absorbance of the DNA template at 260nm is more than 0.05, and the ratio of the absorbance A260/A280 is between 1.8 and 2, which is qualified.
1.2 DNA fragmentation
Mu.g of qualified genomic DNA was diluted to 100. mu.L with TE buffer. And (3) fragmenting the DNA by using a tissue homogenizer, wherein the fragment length is 150-200 bases.
The DNA was purified using a commercial purification kit.
1.3 quality testing of DNA sample libraries
And (5) carrying out qualitative and quantitative analysis on the DNA by using a biological analyzer, and determining that the length peak value of the DNA fragment is reasonable.
2. Preparation of cDNA sample library
2.1 mRNA extraction
Human mRNA is extracted by adopting a commercial mRNA extraction kit, and the quality and the concentration of the extracted mRNA are detected by using a spectrophotometry and a gel electrophoresis system. The absorbance A260/A280 ratio is between 1.8 and 2.
2.2 fragmentation of mRNA
Fragmenting mRNA by using a commercial kit; the mRNA was purified by column chromatography using a commercial kit.
2.3 Synthesis of first and second Strand cDNA of mRNA Using a commercial reagent cDNA Synthesis kit
The cDNA was purified by column chromatography using a commercial kit.
3. cDNA/DNA end repair
The DNA fragment is end-repaired using Klenow fragment, T4 DNA polymerase and T4 polynucleotide kinase, since Klenow fragment has 5 '-3' polymerase activity and 3 '-5' polymerase activity, but lacks 5 '-3' exonuclease activity. Therefore, the DNA fragment can be conveniently and accurately subjected to end repair. And (4) carrying out column purification on the repaired DNA fragment.
The cDNA/DNA was filled in at the 5 'protruding sticky ends and blunt-ended at the 3' protruding sticky ends using T4 polymerase and Klenow E.coli polymerase fragments to generate blunt ends for subsequent blunt end ligation. The reaction was carried out in a PCR amplification apparatus at 20 ℃ for 30 min.
TABLE 1
Figure 349301DEST_PATH_IMAGE001
4. Adding base A to the 3' end of cDNA/DNA sample
A DNA fragment having a cohesive end A was obtained by adding a base A to the 3' -end of the end-repaired DNA fragment using Klenow having 3' -5 ' exonuclease activity, and the reaction was carried out in a PCR amplification apparatus at 37 ℃ for 30 min. The treated DNA fragment was subjected to column purification.
TABLE 2
Figure 567268DEST_PATH_IMAGE002
5. Adding linkers at both ends of cDNA/DNA
TABLE 3
Reactive materials Volume (μ L)
cDNA/DNA sample library 20
2 XT 4 DNA ligase buffer 5
DNA both ends linker 5
T4 DNA ligase 5
Ultrapure water The total volume is compensated to 50 mu L
6. Isolating cDNA fragments of appropriate length
The DNA fragments were separated using an electrophoresis gel, and 150-and 250-base cDNA fragments were excised on the gel in accordance with the DNA molecular weight.
Gel samples containing the cDNA library were column purified using a commercial kit.
7. Quality detection of cDNA fragment sample library
And (3) carrying out qualitative and quantitative analysis on the cDNA by using a bioanalyzer, and confirming that the length peak value of the separated cDNA fragment is reasonable.
PCR conditions were as follows: placing in a PCR amplification apparatus, pre-denaturing at 95 deg.C for 10 s, denaturing at 95 deg.C for 30 s, annealing at 65 deg.C for 30 s, extending at 72 deg.C for 1min, co-circulating 20 times (cDNA sample bank) or 5-8 times (DNA sample bank), and finally extending at 72 deg.C for 5 min.
And (3) purifying the PCR amplification product by a column by using a commercial kit.
8. Amplification of DNA templates
And (3) carrying out polymerase chain reaction on the treated DNA sample by using a PCR amplification instrument to increase the content of the DNA so as to meet the requirement of hybridization with the probe.
TABLE 4
Reactive materials Volume (μ L)
Post-adapter cDNA/DNA sample library 20
10 XDNA polymerase buffer 5
DNA polymerase 1
Joint positive primer 1
Linker reverse primer 1
Ultrapure water The total volume is compensated to 50 mu L
PCR conditions were as follows: placing in a PCR amplification apparatus, pre-denaturing at 95 deg.C for 10 s, denaturing at 95 deg.C for 30 s, annealing at 65 deg.C for 30 s, extending at 72 deg.C for 1min, co-circulating 20 times (cDNA sample bank) or 5-8 times (DNA sample bank), and finally extending at 72 deg.C for 5 min.
And (3) purifying the PCR amplification product by a column by using a commercial kit.
9. Quality detection of amplified cDNA/DNA sample library
And (3) carrying out cDNA/DNA qualitative and quantitative analysis by using a bioanalyzer, and confirming that the length peak value of the purified fragment is reasonable and about 200 bp.
Second, design principle of probe
According to a human reference genome HG19, the gene related to the DNA base excision repair pathway and all coding sequences thereof are obtained by combining Ensembl, CCDS, Gencode, VEGA, SNP and CytoBand databases.
The design of the probe has several major considerations:
(1) specificity of
Stringent specificity parameters may be effective in reducing probe binding in non-target regions, but also limit regions of a part of exons that have a lower degree of reproducibility, resulting in reduced coverage of the probe design. Too loose specificity parameters will directly reduce the amount of data on the target area, resulting in reduced capture efficiency and increased sequencing costs. Therefore, a reasonable specificity parameter is key to balancing coverage and capture efficiency. We consider a probe with a sequence similarity across the genome of no more than 3 as the optimal threshold for specificity parameters.
(2) Stability of binding
The indicators of the binding ability of the probe to the template are the minimum free energy (. DELTA.G) and the melting temperature (Tm), and since these two parameters are somewhat complicated to calculate, the binding ability of the probe to the template is generally indirectly expressed by a more direct and simple GC content. In general, the greater the GC content, the greater the binding stability. In fact, the closer the GC content is to 50% of the probe, the better the capture effect. The higher the GC content, the better the binding ability of the probe to the target DNA fragment is, however, the better the binding ability to the target DNA fragment is to its original complementary fragment (consistent length on the one hand, no mutation mismatch on the other hand), and therefore, the higher the concentration of the probe is needed to promote it to take some advantage in competition for binding to the DNA complementary fragment.
We will prefer to design probes with GC contents close to 50%, and for unavoidable GC extremes we will add modified bases to the probes and thus alter the GC extremes.
(3) Concentration of Probe
As can be seen from the above binding stability, the capture effect of the probes with different binding capacities is greatly different. Too high and too low binding capacity are detrimental to capture, and therefore, different concentrations of probes for different GC contents need to be set in order to maximize uniformity of capture efficiency. Through summarizing historical capture data, different probe concentration parameters are set for probes with different GC contents, the concentration base number of the probe with the GC content of 40-60% is set to be 1, then the concentration base numbers of the probes with the GC contents of 30-40% and 60-70% are 1 x 22, and the concentration base numbers of the probes with the GC contents of 20-30% and 70-80% are 1 x 24.
(4) Position of the probe
1. The optimal probe placement position is 100% coincident with the target region, but due to the effects of randomly interrupted libraries, sequence repeatability, GC content, mutation types, etc., the actual probe design strategy is as follows:
2. since libraries are typically generated by random disruption, to maximize capture of target libraries, probes are typically designed in a tiling fashion;
3. if the target area has a repeated sequence area, the probe design can be extended to two sides to search areas with better specificity under the condition of ensuring the coverage of the target area during design;
4. if the target area is an extreme GC area, in the design process, under the condition of ensuring the coverage of the target area, a probe is designed by extending and searching areas with more proper GC content to two sides;
different design considerations are also required for different mutation types:
1. for insertions or deletions exceeding 60bp, probes need to be designed on both sides of a mutation breakpoint besides the probes need to be covered, and the closer to the breakpoint, the better;
2. for CNV regions exceeding 10 kbp, the optimal probe design strategy is to place sparse probes at intervals, so that on one hand, CNV can be detected, and on the other hand, the sequencing cost can be reduced;
3. for intron regions where fusion occurs, if the fusion site is not defined, then full coverage of the entire intron is required; if the fusion site is defined, probes need to be designed on both sides of the fusion site.
4. For short-chain repeat regions such as MSI and STR, probes need to be designed to extend 60bp on both sides in addition to the probes covering them.
Hybridization of DNA capture probes
1. Hybridization of DNA sample libraries with biotinylated DNA Probe libraries
The cDNA/DNA sample pool was mixed with hybridization buffer at 95 ℃ for 5 minutes, and then maintained at 65 ℃. The reaction was performed in a PCR amplificator.
The mixture was then mixed with a pool of probes at 65 ℃ for 5 minutes. The hybridization reaction was placed in a PCR amplification apparatus and incubated at 65 ℃ for 24 hours.
Fourthly, obtaining the hybridized gene segment
1. Preparation of streptavidin magnetic beads
The separation of the hybridization products was performed using Dynabeads streptavidin magnetic beads. The beads were placed on a homogenizer and mixed, requiring 50. mu.L of beads per sample.
And (3) washing magnetic beads: mixing 50 μ L of magnetic beads and 200 μ L of binding buffer solution, separating and purifying the magnetic beads and the buffer solution by an external magnetic field, discarding the buffer solution, and repeating the steps for three times.
2. Isolation of the hybridization product
The hybridization product was mixed well with streptavidin magnetic beads and shaken for 30 minutes at room temperature. Separating and purifying the magnetic beads by an external magnetic field.
To the beads, 500. mu.L of washing buffer was added, incubated at 65 ℃ for 10 minutes, and the beads were isolated and purified by an external magnetic field. The above steps were repeated three times.
3. cDNA/DNA enrichment sample Release
Mixing the magnetic beads with 50 mu L of elution buffer solution, incubating for 10 minutes at room temperature, separating and discarding the magnetic beads through an external magnetic field, and collecting supernatant, wherein the supernatant is the enriched gene segments related to the DNA base excision repair pathway.
Fifth, PCR amplification and purification
The enriched gene fragments were further amplified in preparation for loading into a sequencing instrument.
TABLE 5
Figure DEST_PATH_IMAGE003
PCR conditions were as follows: placing in a PCR amplification apparatus, pre-denaturing at 95 deg.C for 10 s, denaturing at 95 deg.C for 30 s, annealing at 65 deg.C for 30 s, extending at 72 deg.C for 1min, co-circulating 20 times (cDNA sample bank) or 5-8 times (DNA sample bank), and finally extending at 72 deg.C for 5 min.
And (3) purifying the PCR amplification product by a column by using a commercial kit.
Sixthly, detecting gene mutation by adopting next generation sequencing technology
The next generation of commercial sequencing instruments were used for sequencing and the sequencing results were analyzed using the existing sequencing software analysis package.
Example 1 detection of mutations in MRE11, RAD50, NBN, DNA2, RAD51C, BRCA2 genes
Firstly, constructing a sample library
1. Extraction of DNA
The sample DNA was extracted using a commercial kit according to the conventional DNA extraction method for tissue samples.
2. DNA fragmentation
The DNA sample was fragmented using a DNA fragmenter to a fragment length of 150-200 bp.
3. DNA sample pool quality detection
And (5) carrying out qualitative and quantitative analysis on the DNA by using a biological analyzer, and determining that the length peak value of the DNA fragment is reasonable.
4. DNA end repair
The DNA was filled in at the 5 'protruding sticky ends and blunt-ended at the 3' protruding sticky ends using T4 polymerase and Klenow E.coli polymerase fragments to generate blunt ends for subsequent blunt end ligation. The reaction system is shown in Table 1, and the reaction is carried out in a PCR amplification instrument at 20 ℃ for 30 min.
5. Adding base A to the 3' end of the DNA sample
The reaction was carried out in a PCR amplification apparatus at 37 ℃ for 30 minutes, and the reaction system is shown in Table 2.
6. Adding linkers at both ends of DNA
The reaction system is shown in Table 3.
7. DNA fragment sample library obtained by amplification
The Polymerase Chain Reaction (PCR) was carried out in a PCR amplification apparatus, and the reaction system is shown in Table 4.
PCR conditions were as follows: placing in a PCR amplification apparatus, pre-denaturing at 95 deg.C for 10 s, denaturing at 95 deg.C for 30 s, annealing at 65 deg.C for 30 s, extending at 72 deg.C for 1min, co-circulating 20 times (cDNA sample bank) or 5-8 times (DNA sample bank), and finally extending at 72 deg.C for 5 min.
8. Quality detection of amplified DNA sample libraries
And (3) carrying out qualitative and quantitative analysis on the DNA by using a biological analyzer, and confirming that the length peak value of the purified fragment is reasonable and about 200 bp.
If the concentration of the obtained DNA sample library is low, the sample is dried at low temperature (below 45 ℃) by a vacuum concentrator and then dissolved with nuclease-free water to reach the required concentration.
Secondly, preparing a DNA probe library of MRE11, RAD50, NBN, DNA2, RAD51C and BRCA2 genes
According to the above-mentioned method and idea of designing a probe, a probe having Biotin (Biotin) at the 5' end was designed and synthesized for the test.
Thirdly, hybridizing the DNA sample library with a biotinylated DNA probe library
The DNA pool was mixed with a hybridization buffer (10 mM Tris-HCl, 2% bovine serum albumin, pH 8.0) (after mixing, the concentration of the DNA pool did not exceed 50 ng/. mu.L at most) under reaction conditions of 95 ℃ for 5 minutes, and then maintained at 65 ℃. The reaction was performed in a PCR amplificator.
Then, with a DNA sample library: the probe pool was added to the above mixture at a molar ratio of 1:100 under reaction conditions of 65 ℃ for 5 minutes. The hybridization reaction was placed in a PCR amplification apparatus and incubated at 65 ℃ for 24 hours.
Fourthly, obtaining the gene segments of MRE11, RAD50, NBN, DNA2, RAD51C and BRCA2 which are subjected to hybridization enrichment
1. Preparation of streptavidin magnetic beads
Dynabeads streptavidin magnetic beads are placed on a mixing instrument to be mixed uniformly, 50 mu L of magnetic beads and 200 mu L of binding buffer (10 mM Tris-HCl, 2% bovine serum albumin, pH8.0) are mixed uniformly on the mixing instrument, the magnetic beads and the buffer are separated and purified by using an external magnetic field, the buffer is discarded, and the steps are repeated three times, and 200 mu L of binding buffer is added each time.
2. Isolation of the hybridization product
The hybridization reaction mixture in step three was mixed with the treated streptavidin magnetic beads of step four and shaken for 30 minutes at room temperature. The magnetic beads were separated and purified from the buffer using an external magnetic field, and then 500. mu.l of a washing buffer (phosphate buffer, 0.1% Tween-20, 0.1% SDS, pH 7.4) was added to the magnetic beads, incubated at 65 ℃ for 10 minutes, and mixed uniformly every 5 minutes. And separating and purifying the magnetic beads and the buffer solution by using an external magnetic field, and repeating the steps for three times.
3. Release of enriched DNA samples
The beads were mixed with 50. mu.l of elution buffer (10 mM sodium hydroxide solution), incubated at room temperature for 10 minutes, and mixed uniformly every 5 minutes. Separating and purifying the magnetic beads and the buffer solution by using an external magnetic field, reserving supernatant, and discarding the magnetic beads, wherein the supernatant is a DNA sample library containing the enriched MRE11, RAD50, NBN, DNA2, RAD51C and BRCA2 gene fragments.
Fifth, PCR amplification and purification
The enriched DNA sample library was further amplified in preparation for loading into a sequencing instrument, and the reaction system is shown in table 5.
PCR conditions were as follows: placing in a PCR amplification apparatus, pre-denaturing at 95 deg.C for 10 s, denaturing at 95 deg.C for 30 s, annealing at 65 deg.C for 30 s, extending at 72 deg.C for 1min, co-circulating 20 times (cDNA sample bank) or 5-8 times (DNA sample bank), and finally extending at 72 deg.C for 5 min.
Sixthly, verification of detection sensitivity
Mutant and wild-type plasmids were constructed for MRE11 (c.2092a > G), RAD50 (c.572c > T), NBN (c.425a > G), DNA2 (c.2430c > G), RAD51C (c.186a > C), and BRCA2 (c.1114a > C) mutations in the pathway, samples of different abundances were mixed according to the copy number ratio of the mutant to the wild-type, sensitivity was examined by capturing and sequencing using the above-described probe library, and each sample was tested 3 times repeatedly, with the following results:
TABLE 6
Gene to be tested 5% plasmid 1% plasmid 0.5% plasmid
MRE11 5.12% 1.03% 0.55%
RAD50 5.04% 1.10% 0.61%
NBN 5.19% 0.98% 0.44%
DNA2 4.95% 0.97% 0.53%
RAD51C 4.97% 1.07% 0.45%
BRCA2 5.09% 1.14% 0.60%
As can be seen from the table, the detection probe library and the detection method provided by the invention have better detection sensitivity for low-abundance samples, and can reach the detection sensitivity level of about 0.5%.
The optimized probe library was used to detect 88 pathway-associated genes in 10 tumor samples of lung cancer patients, 8 of which were missense mutated in exon regions in at least 1 pathway-associated gene (80%), and 4 of which were missense mutated in exon regions in more than 5 pathway-associated genes (40%).
Sequence listing
<110> Suzhou academy of health and occupational technology
<120> probe library, detection method and kit for detecting effectiveness of DNA homologous recombination repair pathway
<141>2019-12-02
<160>579
<170>SIPOSequenceListing 1.0
<210>1
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>1
cgaataaacc gagggaagcg aagggagatg cccttgtcac tatccacctg cggaagcggg 60
atggagactc ctgcggtcca gccccagcga ccccctgctc 100
<210>2
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>2
cgatcaccac caggtccagg gtgtcaccca cgccatcaag gtagtccttc ttcagctggg 60
agaaggggag gcaagagatg agaaggggga gcgcccaagg 100
<210>3
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>3
ggaaaggggc tcacgtacca gggactgcag ggccggcagg gagaagagag atgagacatc 60
tttgtgagaa taaagacaat aagccctaaa tgtgaggcga 100
<210>4
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>4
cttccagggc gtggatctgt cacgatggga gaagggaggg gaaatcagct gagtcccctc 60
atgtggcctc agctttctct tctgacccca cctgcactgg 100
<210>5
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>5
cgggaacctc gctggggtgg cgggtgagaa caagataggg gaagcctttc tagaactcac 60
acagtttgga aagagagctc ggcctgctcc cagaagcctg 100
<210>6
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>6
gtccgctcag ggacctgggg agagagcagg ccagggaagg gggcttgtct gcacctcccc 60
aatcttgcct cctcccttct ctgatctcct cgaccttgat 100
<210>7
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>7
ctgaggtaga ggacagggag gaggtctgga ggcgacaggg ccaccacgga gcgcagcaag 60
ttgctcagcg tctccaccat ccggagcctg gaggagggga 100
<210>8
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>8
tccctccttt cctggagccc ctggctcctc ttccttcact tcttttttga ctgctggctt 60
ccggggggct aggaatgaag acagaaaaca gtgggtcttt 100
<210>9
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>9
ctttctttcg gggtctcctc ttctgacgat agacagaacg gtcagatggc aaaaaaatcc 60
cattgaacct acaagggcat tttgactctg gagacagtct 100
<210>10
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>10
gtccagggca ggcttctgga agaggaaaga acagttctag agtgagcggg ggaaggaggg 60
actccatttt ccaaaagttg agagtagagg ggactgaaac 100
<210>11
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>11
gtggaaaaat gacctagagg agcataaaag ggggtaaaaa aaggagaata acagcaccaa 60
taccctgcct atctatgctg tatcctataa cttccttttt 100
<210>12
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>12
actcataagg tcatcagcac taaaggcaga agcagactcc tctgactgag atctgagtgc 60
tctggccctg gtcatagcct aagagggaga agaaggagaa 100
<210>13
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>13
ttgttccttc tgaaggcttt gtgataagtt tcccaaagtt gatctcttct cctagaaaaa 60
aagaagtata tcaaaaaata gcttctatca taatgttaaa 100
<210>14
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>14
ggaattttat gcatattcat cttcctccct ttaatacgca gcaaaccaac atgtctgaag 60
tggagagaaa tgaacaccga gtcacagtgt aaatttcctc 100
<210>15
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>15
ctcaacattg ttactttttt attgacaaac attcgataga gcctttcatc tggaatggat 60
cctgaaatgg acattacatt atttttaaat tgttttctta 100
<210>16
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>16
ctgtgggatc gtcatgattg ccatgaatac taaacactgg aattgaaatg ttgaggttgc 60
catcttgata gttcacccat ggaaacctta aaaaaaaaaa 100
<210>17
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>17
tcatttcctc tgactgcatc tttctccata aatccaagat gaatatctgt tgcaactaat 60
attttaaatg tgttttcatc atcactatat taagaaagaa 100
<210>18
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>18
gtcaataaca gcttttttgg aagcatctca ctatcatcct gaagtttgtc attgttctta 60
aatggggtta agatggatag gtaagaaaga gaagaaataa 100
<210>19
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>19
ctttacagtg ggtgcatctt gtgaaagcat tctgaatttt tgttccattt tggagacttt 60
gatttctttt ggcctttcac tcaaatccct gtagaaaaag 100
<210>20
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>20
taggtctaag accttgccta ttagaataaa atagtttaag tatgataata tattaaaact 60
agcaactttt attcagataa tgtcaagata aagcaacctc 100
<210>21
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>21
atttttactt ccaatagatg gttcatcaag aggtgggtaa aaactgtaaa aataattaaa 60
gtatattcta attatatact actatgtttg ctattaaatt 100
<210>22
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>22
gagacatcta aacaagaaga gcatgcaacc aaaggctcat actctattct gtaaatgaga 60
ataagttaaa taaagtcata gtatcagagt tgcagagatg 100
<210>23
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>23
gatcattttc aatcagaatg gcacagtttt tccttccaac aacgtactca acgccagtca 60
aaagtctgta tggttctcct gagataaatt tttttttaaa 100
<210>24
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>24
aatatctgta ttaaggctga ggcaaagaga aaagacacgg ctatatcatg acattttcca 60
cctagatacc tttagctctt taccagctgg tgtgccacag 100
<210>25
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>25
gaagtgaaga gcatcgatgg caacgatctc agtgcagcgc cgctgccagt cgtccctggg 60
acaggaagga aaggggagaa aagataagga acaggtagtc 100
<210>26
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>26
gacattgtga tggaatgatt catcttctgt ttcaggagtg gtattggctg ataaaagaaa 60
caaaaaaata cagacgagaa gagcaataat tagtttagca 100
<210>27
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>27
cttttggaaa aaagaaaaac atatgaggaa gctattctaa gttgttttgc atcacagaat 60
caaaggtcaa acaagcaagt accaaaccat ttttcagaga 100
<210>28
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>28
tctggagaaa ggtgaggcgg aacgtattta gggatcttcc tttctgttct ttgatgtttg 60
gtttcccatt gttcttttct aaggtcaaga caaatgtatt 100
<210>29
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>29
tatctgtgtc actgtgatca tcattttgag gtgaccgatc aatgttagca ttactaaact 60
gctctggtac cagggttact gtttgaggtt cactttccaa 100
<210>30
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>30
tgcccaaacc tgaaaaacag aagtcacaag tgacatgaac tgtgagtaca gatgtggcaa 60
agcttccagg gagatgagct ctatttgatg ttgggtccat 100
<210>31
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>31
ggagaccatg tcagcgaaat agatcccttt accaaacatg tagcctgtct ggaaggtcgg 60
aaaagggaga gctgagaatc ttctgatggg aaattaggag 100
<210>32
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>32
gaatctctgt caaccacctg gataaacaga atcttgggat tagcacaaaa gagacccagg 60
agagctacag aggaggaatg ctcaggctca gtcaatgcat 100
<210>33
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>33
cctgagacac cgcctggaga ggaggggaca gaaggaatgc aagactgagg gagcagctcc 60
aagcccccgg ccaggctttc tcttttagca ggtgggtgct 100
<210>34
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>34
tcactgcctc ttcatccttc aggaaaaaag cacattgcta agagacccaa atcaacaact 60
caagcaaggt atctgcgtct gtggggctgg actgcagaca 100
<210>35
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>35
gcgcagagtg ttccagtcct gtcccagagg aaaagcacct acagttttct ctccccttga 60
ggtgctagca ctctcacgga gaagggatct gcaggcctga 100
<210>36
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>36
ttcatgttgg ataatggctt atctgggatg aaaggagaga atcattcagc aaggccagct 60
ctggtgctgc tccccagtaa ggggaggtgg aggagcaggt 100
<210>37
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>37
gcatcgctct tgaagaccag ctgacccgag cattcctcgc agggaaggag ggcaccgaac 60
accatgccat cagctactcg gtccaagatc tgcagccagt 100
<210>38
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>38
ttcatccact ccatccacct catcgccttt tctctttctg aaggagacac aggatatgag 60
agacagccag agccattaaa agtctgacac ccaatgactt 100
<210>39
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>39
gagtcttctc tgccttgcta ccaattccat cctggccttt gcctggagaa tcaaacagac 60
agcaatgctc atctcaacag ccccaaaatg cagctcagag 100
<210>40
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>40
ttggcgtact cgactcgata gagcttatcc gaagactccg ccatcctccc ctagctgccg 60
ccaaagctcc ggaagcccga cgccacgacc tagaaacacg 100
<210>41
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>41
tgtttatcaa aaatgtaggg ctttgacttc taattgtgaa aattataaca cagtatctcc 60
tgtaagtatt ttttaaacaa ttctatttta atcaaattaa 100
<210>42
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>42
caacaagtaa atacaaccgt gataatgaaa aggtactgat aaactgtgag taaattaaag 60
cagactataa tccctttcaa atcactgaat ttggtggtac 100
<210>43
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>43
acatgtaaag ggattgtcta attttattaa tttcattgac aaattagatg agattcttgg 60
agaaatacca aacccaaggt aatgactttt catatattac 100
<210>44
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>44
taatgctcct accaaaaaca aagcagagct tttatatgat gaggaaaaca caatccatca 60
tcatggaacg tctattctta cactttttag gtaagttatg 100
<210>45
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>45
ctcaacataa aaatgctaaa atacctaaga aatcaaatga ttcacagaat agattgtacg 60
gcaaactagc taaagtagca aaaagtaata aatgtactgc 100
<210>46
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>46
tatatttgta ctggagattt ccctcctgga accaaaggaa atacatttgt acacgatccc 60
aaggtaatgg tgctagtaca attttgtatt tttataatta 100
<210>47
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>47
caagaagatt ctaattggcc tttaagtgaa ggaaaggctt tgaagcaaaa gtttgatgag 60
attttttcag caacaaggtt tgtaaccctt aaatagactt 100
<210>48
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>48
agagaaaatg gaaaaggttt gtggtggtag aattttgttc tgcttcaaaa ttttgggatt 60
attgtaatga actttatttg aatccatttt gccatccaca 100
<210>49
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>49
agtgaaagac agattaaaaa ttttcacaaa cttgtgagag agagacaaga aggggaagca 60
aaaactgcca accaactgat ggcaagtatt ttgaaataca 100
<210>50
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>50
tacaacaaca cgtacccaaa tggagatgct gaccaaagac aaagtatgat ttttcttttt 60
gttctaatta tactgtctgg tacttaaaat agcctacctt 100
<210>51
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>51
tgaaagtgat ttagacaggc ttaaagagga aattgaaaaa tcatcaaaac agcgaggtaa 60
gttgtctact ttatattatc aggatacttt gacacctttg 100
<210>52
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>52
actcttgggt acaataatgc ctgaagaaga aagtgccaaa gtatgcctga cagatgttac 60
aattatggag aggttccagg taagtttatt gtagtttaag 100
<210>53
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>53
aatttgcaac gtcgtcagca actggaggag cagactgtgg aattatccac tgaagttcag 60
tctttgtaca gagagataaa ggtaagaata tccatacatg 100
<210>54
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>54
gagaaggtta aaaatattca tggctatatg aaagacattg agaattatat tcaagatggg 60
aaagacgact ataagaaggt aatttaaaac ttaaaattat 100
<210>55
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>55
acaacatttg aaggaaatgg gtcaaatgca ggttttgcaa atgaaaaggt atgcttttaa 60
aataatcttc agtttaaata aacgtcttta ttactggaat 100
<210>56
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>56
tcgtggacaa ggtgagtacc atggtgtatc acaaatgctc tttccaaagc cctctccgca 60
gctcttcccc ttatgacctc tcatcatgcc agcattacct 100
<210>57
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>57
aacaaatctt gaccgagaaa acattgaatc tcttgcacat gctctggttg agtaagtatc 60
tcttgcacat gctctggttg agtaagtatc tcacatttgg 100
<210>58
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>58
tgttggtctg ataccaagag taccttcaga ttctggaaag gattttcacg gggttgccta 60
tgaaggagac aggaaaggac cttagcatga caagtaatat 100
<210>59
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>59
tgaaatcttc aaggatatag ccagctcctg ctcgaagctg ggattctgta tactgcttgt 60
tgaaaggagg aatttccaaa aattctatat taaaaaaaaa 100
<210>60
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>60
ctgacgttac tgcgccgttt ccgcttccct tccaccaaat tgtctatggc aggataagcc 60
aaacaggttg gtcaaactct atggtatgag gccctgaact 100
<210>61
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>61
ccacttggct acaacacgga gccctacaaa gctatttcct ggagaggagg catctaagcc 60
atcagaaggg ccagcagcag cctggaaagg aatttctgga 100
<210>62
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>62
ccaggtcacc tgaggagccc ccagtctgtg aaggggaaac ttcagtttca cactcctgac 60
actctacaat tggctcttca gtctcctgca aggaaaaaat 100
<210>63
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>63
cctcaaggaa cactccatgg tttggattcc tatgttattt tggctgggcc tttcaatcaa 60
ggccttacta ggattttcct tattagtact ccgtccttct 100
<210>64
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>64
cccaagtatg ggatcatggg tctccaccat gctttcagac atgacatcac tggttgctat 60
ggtgctttct ggatagttgc taatacctga aagaagtgag 100
<210>65
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>65
tctgctctca ctaaaggtaa ggaaggctgt gaagaatctt gctctacagg ttctgtttct 60
gcagtccctg atttcagatc tccttcatat tctacactct 100
<210>66
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>66
tcccctgtca aagatagccc aagatcctca ggggaattct ttggttcaat ctctgaagtt 60
ttagaacact caactgtcaa agatgaacta tgcatatctc 100
<210>67
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>67
gtagatcgga aagcatcagg agaaggagca acaagatctg aagaattcct ttatataaag 60
agagaatcac aggttatttt accatagcca cagttttgca 100
<210>68
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>68
tccataagtt cttgtgcaga caactgttct tttgcttcca tagcagcaac agatgctttg 60
gggctaaaag gcatgtcctc tgacctagga aattcatatc 100
<210>69
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>69
atatggcaca gtattttcct caacatcctg gctctgggag agttccagaa ccccaaaacc 60
caactgtgat gaggcagtat ctaggaacaa aatgccaaga 100
<210>70
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>70
ctgatggaac cacatgtgtc caagttagaa gaatccacag ggtctgaaaa aaataactgg 60
atattagcat gaaagacatt taccaacaaa ccttaataca 100
<210>71
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>71
gaaatctgaa tccaactgac ttccagtagg gtccatctgc tcccctggaa tggaataaca 60
aaagatcagt tccgtgtaac ctactagcct tgctcacgca 100
<210>72
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>72
agtctatgtc cacatcacac agtcgtcgag aggcatgaga aggttcttct gatgagctcg 60
aggctttctg cattatagtt tgtgtcgttg caaaaagaac 100
<210>73
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>73
gcatttctgt ttttcctgtt ccttctgggc catgaaattc aagaatatca cctgtgtaaa 60
atttaaaaat ctcagtcaaa atgcagtagc tcaagggtag 100
<210>74
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>74
gcaaacagat ttggttctat ttctttcaag gaacttctac cttcaagtcg ggcaaggagc 60
ttataaaaga agagagaagg aaaactcatt atttaaaaat 100
<210>75
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>75
agcggcccgt gtgctgcgcc ctgctcctcc atggcctctg tcacctggaa gagcacagtc 60
caggtcagct gcaacggctg agggtcttct cgatggttag 100
<210>76
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>76
gcagccgcgg ctgctgggcc atgagctgct gcaggcgctt gtgcgggaag gcgtcttccg 60
tgcagatgta gacggctcct gggaagcaag agtgcctgat 100
<210>77
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>77
aggctcaggc gctggtgctg cgtggggaac cgctccttct gctggtgcag ctgcagtgct 60
aaagggcagg gatagtgtca ggcctgactc tcctgggcct 100
<210>78
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>78
cttcaagtct ggtccagaaa agtgtaaaac ctcctttacc gatttcagtt tggctgaaat 60
aacacagata aattacagga aaatgtcaga ctgtcacaac 100
<210>79
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>79
taaaaaggaa gtagagagat ctagaagaga gtaagttaat tctctactta atctctaact 60
tcattgcact aaggtcagac actgtagtct atatgatacc 100
<210>80
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>80
tgcaagccat aattacagag gactcggatc tcctagcttt tggctgtaaa aaggtactca 60
cctctgacta ctatatatta cttttctatg tagatagtag 100
<210>81
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>81
tcccatcaaa aggaaactta ttcctctgaa cgcctatgaa gatgatgttg atcctgaaac 60
actaagctac gctgggcagt atcctttctg aaacagaatg 100
<210>82
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>82
ggttaaatct cccaaggaaa tcatccattg tgaaaagacc aagaagtggt acgtatttca 60
gttttgcaaa atgctggtga atattggtca tatttaaaga 100
<210>83
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>83
ataatcatat tccaggtgat catattccag acaaggcaac agtgtttaca gatgaagagt 60
cctactcttt tgagagcagc aaatttacaa ggaccatttc 100
<210>84
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>84
gagaaagcat cataatgccg agaacaagcc ggggttacag atcaaactca atgagctctg 60
gaaaaacttt ggatttaaaa agtgagttgc cttgtttctt 100
<210>85
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>85
ctcagtgcaa aagttagttg aactggttca ttcatctcta tggtaaccta caaaacaaaa 60
gattcatcat tgaaaaacat caagaaagtt gcaatctatt 100
<210>86
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>86
atagtctgaa actttctcct ggtctaccaa aagaaagcag atgcttttga gaaatactga 60
cacagagttt tgattttctg tagcttcgtg actcggtaaa 100
<210>87
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>87
gtgtaatgat atcttcattg ccggcgcatt ttagtatttt ggacatacta gaagacagga 60
gacacatgct ttaaaatcaa cgccgtctgc tgaagggctg 100
<210>88
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>88
ggcctcgttg atgaggtcct tgagtgcctc caacaccttc ttgaggatgg agccctggac 60
caggcgcgcc tcgaacatgg tggcggagtg gcaacaacgc 100
<210>89
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>89
gcccctcatc ttccaacagt tggagattga ccattatgtg ggtgagttta ggggttatgg 60
gtgagtgctg gggccctgcg ctcctggggc agaggccggg 100
<210>90
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>90
gagtgagtgc tcccccagga tcagcgggtt ggagggtccc ctcgggaggc cattggctgg 60
tcccagcttc ttccatccac aggcatgttt gggtaccacg 100
<210>91
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>91
cggacatcgt cggctgcaac tggctggagc tcccagctgg gaaatacgcc ctgaggctga 60
aggagaaggt gcagggcttc ccagggcagg gctgggtggg 100
<210>92
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>92
cgcctggcgc tcaccctgcg gccctgtgcc cccatcctgg gtgccaaggt gcagagctac 60
gagaaggagg aggacctgct gcaggtagct ctcgctccac 100
<210>93
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>93
cgcgtgcaga tggacatgct gcaggtatgg gcgggaggtg gggtgtgtcc ctgtccttgg 60
aaggccactg cccaggcccg cagcccacca gcccacccac 100
<210>94
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>94
cgagggtcac tggcgtgccc ctcagctacc tgctcagtcg tggccagcag gtcaaggtcg 60
tatcccagct gttgcggcag gtcagtagcc gagacttgtc 100
<210>95
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>95
acctgtgtta caccacgctc cttcggcccg ggactgcaca gaaactgggg tatagtgccc 60
aattcagcat gtgtcccccg aggcccatct gggccttccc 100
<210>96
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>96
ggcgcccagg tgggcaagtt gccgtgcctg gagatctcac aggtgggcac tcgggcccct 60
ggaaggcaac tgggggcagg tgggccccct gtgtaggaga 100
<210>97
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>97
cggactgggt gtcaggtcac ttcccgtcgc ccatccggct ggagtttgag aaggtgcgtg 60
gctgggtcag gggctctgca tttaggtgcc ctcatcaggg 100
<210>98
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>98
atcaccaagg agctgacccg cgcggcctcc gactatgccg gcaagcaggc ccacgtggag 60
ctggccgaga ggtcctgcgc ggggcgggtg gcctggccag 100
<210>99
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>99
tgccgaggct gtgctactgc gtacgggggc accaggggac tgggggcacc ctgggggggc 60
agaggagatc accggcccac cacctgcctc ctctcctgca 100
<210>100
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>100
gaaggaggtg agagggccgg gaggtgagga ggggccaggt ggggaggcgg gggcgccctg 60
ctcagccgct gccgtcccca ggtatcccat ctgaatgccc 100
<210>101
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>101
cctggacctg aggcctggtg accttgcaag catcccatgg ggcgggggcg ggaccaggga 60
gaattaataa agttctggac ttttgctata tggtgctttg 100
<210>102
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>102
ttgtagaagg ggtaacaacc tggaggagaa ggggcagctc tgaggagggt tccgcctcag 60
cactagcact ctgcacagga tgtggggctg cagcccctcc 100
<210>103
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>103
ttggtgggat caaactcgcc tggcatcacg tccacgggca ctgaggcctg gaaggcacag 60
ggcagggaga gctcacaggg ccccgaagga gccccctaca 100
<210>104
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>104
gacaccagta gcacaaacct gcgggagaag gtggggtcct ccagggtgcc gagaagggag 60
gcagcccctc ctaggtgcaa ctcaaagatt ccactgacca 100
<210>105
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>105
ctgggtgtat gtatttactc cgaggaggct gggggagcag gttgtgctgg aatgcagaga 60
caggaggtat aatcttgggg ccagagccca ccaaagctct 100
<210>106
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>106
tgtgggccct ctgggcagcc tgctcagaaa acatggccac actcctgact tgcttggtcc 60
acacagcttc gcccaggcca aggaggttca ctgcgaaaac 100
<210>107
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>107
cttgcagaac tgcagcaagt aagcgtctta atgttccttg tgggcttctt tacgaattga 60
ttttgtaaga tgtttacaca gtggctgcct cttaagagtg 100
<210>108
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>108
atccagcaaa aaagcagagc ctgttaaggt gctgcagaag gaaaaaaaaa ggtaggaaaa 60
ttttgttgct tattggggaa gagtctttac tgggggtgag 100
<210>109
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>109
ccgtctccac ctcttgaacc agtgccaaag actgagcctg aacctccttc tgtcaaggta 60
aaattatact gggattcttg catgtccatg catcctttgt 100
<210>110
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>110
tgggcaaagc caacagacag gtgtccatta ctggcttctt ccagaggaaa taaactgcca 60
tctctggtag atcagagact tggagtggtc aagggagaag 100
<210>111
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>111
accagcgctg cagccgtgtg atccctgcca gggtgggaag aagactctgg agacttgtgg 60
ggggtggggt agagtctgag ctggagaatg tgtgtgttgg 100
<210>112
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>112
tggggctcct cccctgcaat gacagctgct cttcagtacg aggttggctt cagtctcctg 60
cccctcacct cccagccttc ctcaccaccg ctttgcctct 100
<210>113
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>113
tccctcctct tcacaaccgg gtaggaatca gtgatgagcc gcttccggcc catggcggcc 60
acccaggcag gcagagaagg aggcaactgc ggggaagagg 100
<210>114
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>114
ctgtgtctag atgcctttat agcttgaagt gagtatggag taaggtgggc ttccttattg 60
taagctcttg tgaaggctgc tttcacctat atgaaaggag 100
<210>115
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>115
aaactgggtc atgtaccccc tcatcaactc tccaggaaga acactggcat aaatggcctg 60
aaaaaaaatc aattgcagtc cagaatttat gttccgtaca 100
<210>116
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>116
atctgacttg tccacaagtg acatgtgagc agtctcctct ccagctgcaa acactttccg 60
ggcaacatca aatgggccct gaaaaaagag agggacacac 100
<210>117
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>117
cttctttaaa tgcaatagac atcatagcac cctgaaattg acaagggagg agtcctcaat 60
gatttttaac tcatataatc aggtatttta aaaatattta 100
<210>118
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>118
agaggctgct ccatctgacc caggttgagg agcaatggga tgagacaaaa acaaaataac 60
aattgctcaa aaactgctgc tctgtagaaa attatgtaac 100
<210>119
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>119
acgctgcttt aaaactagag ccatcatctt tgccggaaaa tttaccaaac tttgctgcta 60
ggaaaaaaga agttccagag tgtcaaccta tatcacctaa 100
<210>120
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>120
atcagattca acaggccatc ttcatcaata atttttgtcc ccaaggctgc ggcctgttga 60
ttaaaaatgt aaaccatcaa aactgcatcc tgcatcctac 100
<210>121
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>121
cagaatcttc aggacttaca gactttggga acagggaaag gaaaatgaac aaagtcatac 60
agaattcatg caaaatttaa aatacatatctagaaataaa 100
<210>122
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>122
gtcctttcga gcctaaacaa attttaaaag tgtcaaatgt gacaaataat agaaaaacta 60
ttaccataaa atctgttgtt aaaatccatc aaaattttaa 100
<210>123
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>123
ccaggtttag aagatactgg cagtttttct ggtggctttt tggcattttt tacctgcaac 60
gtctcctctg actctgaatc tgtatgtgag agaaaaataa 100
<210>124
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>124
tcttctggtg agtagcccag atgccacaag tgagcaagaa tctagacaaa ggagacagaa 60
aaggctgctt accactttaa actgtaagaa gacatgtgat 100
<210>125
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>125
ggtcagcttt gtgtaccgga ggactgcaca gcgggactga atgggctctg aacagagacg 60
ggacagtagt gaggcttccc tgcagaggcc ggcatcatcc 100
<210>126
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>126
cctcacaacg tcaatgcccc tgaaagaatg acaggttttt actggcacct tctgagacca 60
attcagttgc cttcacaagg acttttaaac aatcggtata 100
<210>127
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>127
tgttgggcac atttccttcc cttgcaaaga cctacggcga aaatgatcat taaaaccggt 60
taaaacttgc ttctggatga ctacataaaa aagatagaaa 100
<210>128
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>128
aactttatgg tgttggagtg gaaaaattga gaattgaaca tcagaccatc acagtaagca 60
tttcactttg aggccctgaa agtaatttat agcggggact 100
<210>129
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>129
tctacatcta aagtgatccc acctattcgt agtaggtgct tggcggttcg tgtgcctgct 60
cccagcattg aagatgtagg tcaagttaca cttttctgaa 100
<210>130
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>130
tatctgaggg agactgcaaa tgctattgtc agtcagcaaa ctccacaaag gtaccagtat 60
aaaatgatga cagtaaagct ttcattatca ggatatcata 100
<210>131
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>131
acttggaagc gtttgtggcc aaattcatgg cactttataa gaagttcatg gaggatggat 60
tggaaggcat gatgttctga cttctgtcag ttattcttgc 100
<210>132
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>132
aatcctataa taaaaaaaac ttttggtatg atgacttaat attcctttcc ccaaagttag 60
taagctgact ttaccttgac cacagcttct agtttgtcaa 100
<210>133
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>133
gcttttctta agtctccttc tgacacttta acaagataag ctattccctg aagagaaaag 60
agttgttttt aacctatgat ggccaattaa ggatttataa 100
<210>134
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>134
tcatagaatc tgcttcatcc agaatcacaa tcttaaaagg cggacacggc ttcccactga 60
ttcagagaaa cacataagag ttgaacatta atatgtatat 100
<210>135
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>135
ttaagaaatg cttgcatggt acttcaccct ggtcaggtgc aagacagaaa aaaaaagctt 60
ttattgaaac ttttattgcg aatcagcacc ataggaaagc 100
<210>136
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>136
tcctgaactc atggttcccc gcctggaaca tgtcgtggaa gaagagaagt gagtattttg 60
cgggcctttg gggatggagt gtagtagaaa caggcttgtg 100
<210>137
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>137
aacatcctgg actggatgtt gaatcaagat ttcaccacag cctacagaag tatcctttct 60
catgacctcc tggccaccga gacctgaagg tggccccagg 100
<210>138
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>138
cattgctgca tttcaagtca ccagagacct gattgttgca gaggcctaga tgctctgagg 60
gccattcaca attctcaggg ctcagcagtg atgggagaac 100
<210>139
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>139
aagcagttgg agtgaagatt ggcaatccag tgccctataa tgaaggtaaa atgctttggc 60
gtaggttgta gcactcaaat gaatacctct ttatatattc 100
<210>140
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>140
cactccttac cagtccaagt gagttgttgc atagagtaag ttcagagtgt acttatgaaa 60
tcggagaagc tattatggac tcttgagtta tttatgcgtg 100
<210>141
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>141
tcacggggat tgatgacctc gagaacaagt cgaaagactc acttgtaggt aagctcgtgt 60
atgagaagga agagcaggga tgactagctt tctttgcagt 100
<210>142
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>142
gagcctctcc gtgctgtctt caagcactat cattgcgaat cctgacatcc cagaggccta 60
taagcttcgt ggatggtagg ttttgtgggg ctaaacaaag 100
<210>143
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>143
agaaagtgat tgatcaacag aatggattgt accgctgtga gaagtgcgac accgaatttc 60
ccaatttcaa gtaccgcatg atcctgtcag taagtagcct 100
<210>144
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>144
gatctttcat attcagagtc agggtcaaag tggagaccta caacgtaagt aagggcctgg 60
gcagcagggt tggtggtggg gaggtgctgt ttgtcaccta 100
<210>145
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>145
aaagttcaac ccttcaggtc ttggacaagc cttaatcaaa ttcaacacct gaagattcaa 60
atcagaaaga ttttagcaat tattttggat cagcctcccc 100
<210>146
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>146
taggtgctct ccctgctgag ggctgaatta agaaataagt tagcaatatt agaaaatcca 60
tcaagttaaa cagtctttac tacttaaaat agctcgttaa 100
<210>147
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>147
cacaatgtgc tgggctcggg ctctctgaaa agaaaaaaca tgcaaaattc aattaagaaa 60
gtaataacta ccactcattc aacacctgcc atgtgccaag 100
<210>148
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>148
cctgttttga acaacaggat tagtgcctgt gccacgtccc acgcctccga gaaacccgca 60
ggctcccgga ggcttcgccc cttcaaacac tgcccgagtc 100
<210>149
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>149
gatctgaaag aaacatttaa gcaaacattt aatctacaat ggaaagttgt aacatcaatt 60
attatctaag atatttttac ctatttcctt tttctcaccc 100
<210>150
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>150
ccttggcggt tactcttcca accacttcca caattccaga gatttcttca tcaagctaag 60
acacagaaca agacatcgat ttggtgatat cacattttca 100
<210>151
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>151
agcatgccgg cgttgatgcg cgacctgggc aagtccatca tgtccaccat gattatggtc 60
caagactgcg gctggcggga aacccacgga cgactgaaac 100
<210>152
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>152
tgtttgaccc tgtgttcaag gttaggggaa ttatagtttc ccaggtctcc atcgtggggg 60
taatcagagg ggcagagaag gcttcaaatc acatttgtta 100
<210>153
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>153
gggctcagct ctgcgacctt agcgtcaagg ccatcaagga agcgattgat tatctgaccg 60
ttgagggcca catctatccc actgtggatc gggagcattt 100
<210>154
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>154
tagacatgct tttacccctg acaaaaacac aagaattaaa gcaaactaag tatcaagtga 60
gcactatatc tctctcatta aaatcaagca atttacctaa 100
<210>155
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>155
acgctgccca gtgttcatct gttaatataa aaggagatac cagtgttaaa aacattagac 60
gactagacaa agacattaaa cagactgtta cttgatataa 100
<210>156
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>156
attttgatac ccggtggtgt tcaccaatgc cttatgctgg agcaataatt ccactacctt 60
caggtgccca tgattgcaag cttcatgcta attaaatttt 100
<210>157
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>157
tgtttttcct ccctgatgta ccaccaactt tacgtttgca tgaaggtggt gaagaacatt 60
caggcaatgg tatattttct gagggcaccg tttgcttaac 100
<210>158
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>158
atttatcttc aagtcttgta tccaggccgg ggtgtaacac actggacatc cagttccaat 60
gcagtcactt acacaattac tttaaaataa ttaaaaaaaa 100
<210>159
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>159
cagcaggaca ggaaacacag agaggtggat caaaatcatt attgccagat ttcttgcaga 60
ctccgaagga agttgtatgc actacccaaa acacaccaac 100
<210>160
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>160
tccagaagaa attatttctg cttcttcttc ctcttcaaaa tgccttaggt aaactagcta 60
aataattagc attattattt gtttctggga tactttaaat 100
<210>161
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>161
attaattgat actattcctg atgataaact gaaacttttg gattgtggga acgaactgct 60
tcagcagcgg aacataaggt atcttaattt tcccccttct 100
<210>162
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>162
tgtcatctac tgctcaaaat ataaacttct cagagtcaat tcagaattat actggtaagt 60
ttaaaataaa ttgaatgctt atatgaaaac aaaactgtcc 100
<210>163
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>163
cttccttgga tgtaagttat aaaaatacta ataaaaacac gccttagaaa caattaaatt 60
tcagtcctct ggataacctt tttattaaat agttcgtgat 100
<210>164
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>164
ggaagctctt ggcacgtttt gttattgatg aagcacattg tgtcagtcag gtaaatactg 60
ttttttatat ccggaaatac cgataaatac atactaccaa 100
<210>165
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>165
agcctaaaaa ggtggcattt gattgcctag aatggatcag aaagcaccac ccatgtgagt 60
acagccatgt gattagctgt ctagaagtaa caaatgtctt 100
<210>166
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>166
gctggaagag atggggaaat atctcactgc ctgcttttct atacctatca tgatgtgacc 60
agactgaaaa gacttataat gagtaagctg ggctccattg 100
<210>167
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>167
ctggaagatt tactatgaat atgctggtcg acattttctt gggtaagtca tctgttttga 60
atgtttgagt tacttcaatt gaaattgaac atctaaagaa 100
<210>168
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>168
tccattactt caatattttt aataccgtca ctctcaagaa gcttgcaggt gggtacacat 60
gtatcctttg ttacgtggca cagattaata ggccgaaagt 100
<210>169
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>169
gaaatgaaag gaagaggaaa aagatgccag cctcccaaaggtctaagagg agaaaaactg 60
cttccagtgg ttccaaggca aaggggtatg ttttgtgaca 100
<210>170
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>170
ggtttcttcc attgaccaca tctcctctga cttcaaaatc atgctgaaag aaaccaaaca 60
caacccatca ggataagaga aagagaagct tccttcaatg 100
<210>171
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>171
aatgcagagg ttgaagatgg tatgttgcca acacgagctg actctggggc tctgtcttca 60
gaaggatcag attcagggtc atcagagaag aggctgattc 100
<210>172
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>172
gtgaagttaa tactgcttta aatggaatga gaaaacaaat ctactttact gctttgttct 60
gatagtgata attcaggtta gaatactgat ttttttcaaa 100
<210>173
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>173
cttcagagac gcttgtttca ctctcacacc cagatgctgc ttcaccttaa ataacaaaaa 60
cagaggttca gatgtaaaag cagactataa acgctgcaac 100
<210>174
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>174
atgactactt cccataggct gttctaagtt atctgaaatc agatatggag agaaatctgt 60
attaacagtc tgaactactt cttcatattc ttgctttttt 100
<210>175
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>175
ggggttttca aatgctgcac actgactcac acatttattt ggttctgttt ttgccttccc 60
tagagtgcta acttccagta acgagatact ttcctgagtg 100
<210>176
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>176
tagattttca gttacatggc ttaagttggg gaggcttgcc ttcttccgat aggttttccc 60
aaatattttg tcttcaatat tactctctac tgatttggag 100
<210>177
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>177
cttttttgca gaatccaaac tgatttcatc cctggttcct tgaggggtga tttgtaacaa 60
ttcttgatct cccacactat agggaaaaga cagagtccta 100
<210>178
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>178
ttagatgttc aggagagtta ttttcctttt ttgcaaaatt atagctgttt gcatctgtaa 60
aatacaaggg aaaacattat gtttgcagtt agagaaaaat 100
<210>179
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>179
aaactaaaat ggatcaagca gatgatgttt cctgtccact tctaaattct tgtcttagtg 60
aaaggtatga tgaagctatt atattaaaat atttaaatga 100
<210>180
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>180
tgctcatagg taataatagc aaatgtgtat ttacaagaaa gagcagatga ggttgataat 60
tgtcatctct aatacttctg ttaaaaggaa atatgaaaag 100
<210>181
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>181
aaatctacaa aaagtaagaa ctagcaagac taggaaaaaa attttccatg aagcaaacgc 60
tgatgaatgt gaaaaatcta aaaaccaagt gaaagaaaaa 100
<210>182
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>182
tttgaagcaa atgcttttga agcaccactt acatttgcaa atgctgattc aggtacctct 60
gtcttttttt ttttgtaaat agtacatata gttttataga 100
<210>183
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>183
atcaagaaga aactacttca atttcaaaaa taactgtcaa tccagactct gaagaacttt 60
tctcagacaa tgagaataat tttgtcttcc aagtagctaa 100
<210>184
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>184
tgctggcctg ttgaaaaatg actgtaacaa aagtgcttct ggttatttaa cagatgaaaa 60
tgaagtgggg tttaggggct tttattctgc tcatggcaca 100
<210>185
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>185
gggttttcat acagctagcg ggaaaaaagt taaaattgca aaggaatctt tggacaaagt 60
gaaaaacctt tttgatgaaa aagagcaagg tactagtgaa 100
<210>186
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>186
ataattttga ggtagggcca cctgcattta ggatagccag tggtaaaatc gtttgtgttt 60
cacatgaaac aattaaaaaa gtgaaagaca tatttacaga 100
<210>187
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>187
agacaaacaa cagttggtat taggaaccaa agtgtcactt gttgagaaca ttcatgtttt 60
gggaaaagaa caggcttcac ctaaaaacgt aaaaatggaa 100
<210>188
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>188
acatccactc tgcctcgaat ctctctgaaa gcagcagtag gaggccaagt tccctctgcg 60
tgttctcata aacaggtatg tgtttgtcta caatactgat 100
<210>189
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>189
tttgctaata gatgcctaag cccagaaagg gtgcttcttc aactaaaata caggcaagtt 60
taaagcatta cattacgtaa tcatatacgg cagtatggtt 100
<210>190
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>190
tgttggttgt gttgatgtaa ttattcaaag agcataccct atacaggtat gatgtattct 60
tgaaacttac catatatttc tttcttttga tacaattaat 100
<210>191
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>191
ctttatgaag cagtgaagaa tgcagcagac ccagcttacc ttgaggtgag agagtaagag 60
gacatataat gaggcttgat gattattcaa ggtgagaagc 100
<210>192
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>192
agtatcaaca actaccggta caaacctttc attgtaattt ttcagttttg ataagtgctt 60
gttagtttat ggaatctcca tatgttgaat ttttgttttg 100
<210>193
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>193
ttatttgctg gagatttttc tgtgttttct gctagtccaa aagagggcca ctttcaagag 60
acattcaaca aaatgaaaaa tactgttgag gtaaggttac 100
<210>194
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>194
ttacctccac ctgttagtcc catttgtaca tttgtttctc cggctgcaca gaaggcattt 60
cagccaccaa ggagttgtgg caccaaatac gaaacaccca 100
<210>195
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>195
atgacccgag ctcaggtgtt gccttcggta ttttaccagt aaaatactgt cccaaagaat 60
taaagcttga ccagctaact ctctttgttt gtttgttgaa 100
<210>196
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>196
actagaataa gagctcccca atcatttctg tgtctaatac atctagaaaa aatagggaaa 60
aagtcaaata attataacat cggaaataaa tccagttttc 100
<210>197
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>197
tcttaaacaa cagaaaaaag catatccaaa attctcagaa attgcttatt cttgtcattt 60
tgaaaatacc tgatttataa ttatagtaat tacagtagca 100
<210>198
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>198
cacagagatt ccgacccttg gggcctgacc caatggtacc aacccaaacc tagaatatga 60
atatgtcatt attagagtta tgcctgaaaa aggcatggaa 100
<210>199
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>199
tttgtccagg agtaagtctg ttgaatcgca attttataat catctgcaaa tctagatgca 60
aagaaagtgc taattaagtg gcaaaacttt taaaacctat 100
<210>200
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>200
tttcatttcc actccatatt ttacaagctg attcataatc tctttctaca agatattcag 60
cgtttgcttc taaccaactg aaataaaata aaacaattgt 100
<210>201
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>201
gatcactaat tttatgaact ccatgataaa aatagcagga ttttccctag aaacaaatat 60
gcataactga aatgtgaacc aatattagca tagaaggaat 100
<210>202
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>202
tccaaattgt gtacttctgt tccaaagcaa tgacgttttc taatctgtaa acacagaacc 60
aaaatgaagt ttaaggtgaa ctagaagttt aactggctag 100
<210>203
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>203
caacatgaca attgtacttc agctttttca cttacgccct catctgctgg tttccctaaa 60
aatgaaagaa catctattta taatatatct aattaaataa 100
<210>204
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>204
taaatcttca ccccaccaat tgtatattca gaccacattg aagacatagt gctttcctgt 60
ttatttcaga ttcctaacta caacagaaat gaaaatgtca 100
<210>205
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>205
tgcctgaaag ctgaattaat aaaatactct ttcaggtttc atcatatgga ctatatatgg 60
caatatctgc aattacaagg caatctttct gtggtggatg 100
<210>206
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>206
atcgataatc tacacaggat taatgtaaaa ataaacatgc atttggaaat tgaaggaaga 60
cgttatttga atagagatca atagaagaaa aatattatgc 100
<210>207
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>207
ggcgatctgg acggctggag tatgccaaga ttaaggatcc aataagtcaa ttctaaaaaa 60
gtttagacaa ccactacaaa acataatatc ttccgtaaat 100
<210>208
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>208
cctcttttca ctatactcaa atgcagtcaa gaatcctggt tcctacagaa agataaaagg 60
aaatgttcaa aaagagttta aagatcatgg ttgtattttc 100
<210>209
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>209
cttcttgttg tcatctgtat acctttgatg gtacagattc ctactgattt cagtttctta 60
atgtcagcca cgttctgtaa attaaagaat gggcacagca 100
<210>210
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>210
tggttatagc aacattaaga cgtcgccaat ctttcaagag ttcaccaacc tgtaagaaat 60
ataattttca attattcaag ttcaaaggaa tggttaaata 100
<210>211
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>211
ctggcgctgg aacctaagtg gaaaaatata cagcctgctg ataatacaat ctctaaagag 60
tactataagt acgcaactgg ccataaagac tatactaccg 100
<210>212
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>212
tctgtcaata aatcagattc atgtttatca gtgtactaaa tctgttcctt gaaaataatc 60
tagtttaaaa ttaacactta agtgtctgcc taccttgctt 100
<210>213
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>213
ttcaaaagaa tattgtcaac agcagagtgt gtatagctgg tcaacaaaac gctaaaacca 60
caggcgtaga gaattcttac ctaataatgg gtaagagaaa 100
<210>214
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>214
aataattaaa tctcgaagtt ttttgctgaa aagtgaaaaa gcacttttag taatattcct 60
tagaaaagtg gagtgaaatt caaatttgct cattgttaca 100
<210>215
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>215
aattatgtaa atattgccca tcacaaacta tctttacatg ttccattcta atcaggtttc 60
caatgcaact gccactcttc tccctatgaa aagaccaaag 100
<210>216
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>216
taataattct atgaaaaaaa aggtgaatgt ctttttaaga gttaaataaa gtttatcatg 60
tctttttcac tacatatgta aaacttgcag atttaaaaac 100
<210>217
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>217
aggggaccaa atgctttctt caatatccat tggtttcacg acttcaatgt tacatgttga 60
attatcctta ctattatcac ttggcctgaa aaaaaaaaaa 100
<210>218
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>218
aaacacctca tggagaaccg taccaattag catttggcgt gtggctggat cagagctcta 60
caaaagcaaa tcacacagtt tatttcacaa catattaaca 100
<210>219
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>219
cagaactgtt cttggaaagg aagctaccac tttcttctga aatctaaagc acacacatac 60
aaaactattt tagcacaacc atgaaataag aagccatcca 100
<210>220
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>220
gcgaacaact ccttaaccga ggctctggag gaagccaacg tcctctttga tggcgtgagc 60
cgaaccagag aagcagccct cgacgcccgg tttcttgtta 100
<210>221
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>221
ttgttgcaaa cctactttcg aaagtatcct gatactcctg tgtcctattt tgagtttgtg 60
attgatccaa actctttttc tcgtactgtg gagaatatat 100
<210>222
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>222
agccgagtca gaaggtccag ggaagaggct cacacggatg ccggcagcag agacaagcaa 60
ggcagaagga aagcaccctg agaagacagg aaagaaagaa 100
<210>223
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>223
gcaggtgggc agagccagag ggtgggagga cacggaacag agggctgact cttactagga 60
agcaagtatt aatgcagttt ctgccccttc atgcagggaa 100
<210>224
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>224
taaacagacc aagaagcagc agcagagaca accagaggcc agcatagggt ccatggtatc 60
cagggtagac gctgaagagg taagaacgtc ctgttgcctg 100
<210>225
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>225
tcagcagctg aaccgagtca gcctggaaat ggccagtgca gttgtgaatg cctatccctc 60
cccacagctc ctggtacagg tatgctgctc cagggctcag 100
<210>226
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>226
ccgacccctg cccccgcagc gtgagtggtc gcgggttccc gagggccagc cgcgagttgg 60
ctgttttgct tccatgattt cagccctgga gctgtccctg 100
<210>227
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>227
ggatgcctac ctgtggtacc gctcactctc atgcccacag cagggctggc tgggacgggg 60
gttcagggga ggtgggcaca cttctggggt tgctgtggca 100
<210>228
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>228
gttaccaagg ctctcgccca gtatcccctc aagtgcgtga tgccaaggct gaaggggggc 60
aggatcacct caaggtagtg gtgcctcccc ggccttttgg 100
<210>229
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>229
catctgcctc ttcctgacca cagccaaccc tgatctcctg ctggacctgg gctcctgacc 60
acacgtggga ccaccaggac agcatgcagc cttggggaca 100
<210>230
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>230
cgcccagctt cctcggggct gggataacag gatacagggc agccgggagg tgaggtatca 60
gattatagat ttgcttcatt atggtcagtc ataagaattg 100
<210>231
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>231
ttcagtacgg gattgcccct ctggggaggg acgaagggca gaagccatca atagggatga 60
cccttgataa ccacagggcc ctcctccacc tcttcttgca 100
<210>232
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>232
gctggcggcc ctgagggctg gggcaccccc tctttgtcct tcccagggtc catctggagc 60
ctgaaaggga aggtgccagg agcgagtgag ccactggcgt 100
<210>233
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>233
tctagaggag cttgtcccgc tgctgaaata aagtgtttag ttattgatga agctcataaa 60
gctctcggaa actatgctta ttgccaggta ataattttgt 100
<210>234
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>234
taattctggc aagagatcag tttaggaaaa acccatctcc gaatattgtg gtaggtattt 60
ttaaataaat tttgatgaca gattaaattt tcactgaaag 100
<210>235
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>235
acgtacacgt agtacttcag caaatggtat ttctgctatc caacaaggtc tggtttttct 60
tttaaaatta tgtattgtgt tgtgtttctt aatcacagtt 100
<210>236
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>236
gtaatgactt ttgtcggcca tgcctcaggg aaaagcacga agggttttac ccagaaggag 60
caactggagg taattatttt tggaattgat aaaaataaaa 100
<210>237
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>237
cttgcagcga aagtcatcta tcttttccta tagggatggt aaataaattt tgcatttgac 60
acatgcattt ttcccctgtt cttacttagc aggtcgtatc 100
<210>238
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>238
ttttataggc cttatgcaaa tgatagaggg aatgagacac gaagaggtgg ggttttattg 60
taactttctc ttgctggtat gatagtaaaa ctagagtact 100
<210>239
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>239
gcctctcagt ggactctcag acttggaata tgaaattgct aagggtactg cacttgagaa 60
tttgcttttc ttaccctgtg cagagcattt acgaagtgat 100
<210>240
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>240
tttaccagtg caaaaaaaag ttatgagtac accactctct aaatcaaaca cattgaactc 60
attttctaag ataagaaagg aaatacttaa gacaccagat 100
<210>241
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>241
agtgaagttg attctccact tcatgctgtc aaaaagcgca gatttcctat aaacagagta 60
agtaaatacc aggtaatgta tagtaatcca aattcctttg 100
<210>242
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>242
gatgagtcag aaaatgaaca agattcctca ttacttgact ttttaaatga tgaaactcaa 60
ctttcacagg ctataaatgg taaatgttat aatgatcctt 100
<210>243
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>243
ggaacaaaat tgtgcacatt caaaaaagaa attatccaga attattttac cagatgattc 60
aagtgaggag gagaacaatg taaatgataa aagagaatct 100
<210>244
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>244
ttgcaagtag aagtttgtcc tcttaatggc tgtgattaca tcgtgagtaa tcgcatggtg 60
gtggaaagga ggtctcaatc tgagatgtta aatagtgtca 100
<210>245
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>245
agactgaaat ctgatatata atcaagctgc tcaagatggg gttttcaaag acctctcaca 60
atattaaatg cacttcaata atcattgctg ttttatgttt 100
<210>246
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>246
acctgatggg catgttctac cgcaccattc gcatgatgga gaacggcatc aagcccgtgt 60
atgtctttga tggcaagccg ccacagctca agtcaggcga 100
<210>247
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>247
tcctgctagg cagtgactac tgtgagagta tccggggtat tgggcccaag cgggctgtgg 60
acctcatcca gaagcacaag agcatcgagg agatcgtgcg 100
<210>248
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>248
ccaagggatc cactaagaag aaggcaaaga ctggggcagc agggaagttt aaaaggggaa 60
aataaatgtg tttccccatt atacctcctt caccccagaa 100
<210>249
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>249
tctcagaaaa cagggagatc acattttaaa tcagtcttaa gagaggtgag cattcagatt 60
tgattcagta attccgattt gaattaaagg gtgcatttta 100
<210>250
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>250
ttggctgtga ttatctccca aaggtaagct gaaattgtca tatttatttg ctattcataa 60
agtctttttc gtactttatg tgctgtataa atatgaatat 100
<210>251
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>251
aatactgctg tccttgtgag tggcaccgta cagaacatga taggcaactc agtgaagtag 60
agaacaatat taagaagtaa gtttttttaa aaactcatga 100
<210>252
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>252
caactacagc caattcggta atgtaaagaa ctgtatggtg aaggtggtgt ttttacttga 60
gtattttata attaaaatat tcctaatatc tttaagctcg 100
<210>253
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>253
ttgcttttac ctaaaaatac tccatgtttg aatgcacaag aacagttcat gtcttctcta 60
agacctttgg ctatacagca aattaaagct gtcagtaagt 100
<210>254
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>254
aacagctgaa aatgaagaaa gctgtttccc agattcaaca aaaagttctc tgagttctct 60
acaatgtcat aagaaagaaa acaactctgg tacttgtttg 100
<210>255
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>255
tactcctggg aggcctgggt ggccttcttg ccgcccgagg gcgccttcgg ccccacggtg 60
gcgctggtct tcttgggcag cagcacggcc tggatgttgg 100
<210>256
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>256
ccttggcgcg ggccttgccg ccagtcttgc cgcggcccga catgctagcg aggtagaccg 60
gtgaagcacg acggctcaaa cactagaaca gacgcccgcc 100
<210>257
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>257
gacttttgta acctgcactg tataactgtt ttgctcgacc ctgaaaagtg ttacaaaaat 60
cagtaggata atagtttggg attagaaaaa agaaaattaa 100
<210>258
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>258
aggtataaag aacaaaagac agatatagcc tgttgacaac gttcttgtcc accttctaga 60
aagacacaat caaataaatt cgtttactag acctgctaaa 100
<210>259
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>259
cttcaagtct ctgtacagaa tgtcacaata agctaaatct atagttccta aaagaaagat 60
acaaatattc aatctttcac aattaccaga ggggcaaaga 100
<210>260
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>260
tgaacaagat ggctgtaaca gttttccaat ggtttagtta ttaactccaa tacctataaa 60
ggttaaagca aaaccatgcc ttgtgttatt attaaaaata 100
<210>261
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>261
cttcaagttc ttaatcacct cacatttttc tttctcctta tgtttcagat attccctatg 60
aaaaagatct gttagaaata tgagcccttg atcttttagt 100
<210>262
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>262
tcaacttcat atattgtatc atttattttc agatattctt ttaactcaac cttgaattaa 60
aaggacattt gcaattaata cgacatataa attcatcttt 100
<210>263
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>263
agagtgattt cttttccctt cttttagttg gaggaaatct tcctttgctt ccagcatatt 60
cttcaacaaa gaaaccgagt tctataccaa aacttgacaa 100
<210>264
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>264
agagtgtctt tgagctgttt acttctagaa aatattggtg tctgggcctt cgcatttcca 60
gtcatggcat tttttagatg atctttaata ctttttctcc 100
<210>265
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>265
ctcatctccg ggcacgagct cggccgcggt gggagcgcca aaaatacacc ccaagcttgg 60
acggttcctt ttggggagag acacccgccg gcggatgcgg 100
<210>266
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>266
aattttgtca tggaggatgc actgaacttg ggcatagcag aatttgataa agaaatatcc 60
gacatctcat gtgatgaaac aaaactaaaa attaaaatta 100
<210>267
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>267
atcatcaaaa tcatcccaaa cttcatttcc caattcaaag ttcacattca aaacttctga 60
aaatagagaa ttgcttttat ctgcatctgt caaatgttgg 100
<210>268
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>268
ttgactcttt aatttctgac ttctgtgcaa ctccaatttt acctatgaaa agaaacttca 60
gattctttag gttaaacttc agatcttatt cttataaagt 100
<210>269
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>269
actggaaaat gaaaaaaaag tacacaaatg tttatttgtc atataaatcc acatatgaga 60
acacttattt ctttttatct ctcttacatt tttataactt 100
<210>270
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>270
ataacagtca ctaatatttc tgccgtcgta tcactatatc ttgtaatctt taaaaaagga 60
caagtataaa aagtgttaaa aataacctta cttctcatac 100
<210>271
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>271
aaccagcatt tacgattgca tttgacaatg ttatacctgt ggaaaattaa tcaaacaaca 60
acttaaaaat aatgcacttt aaataaaaca tatatttaaa 100
<210>272
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>272
ccatgtctga aaatctttgc ggtatcttgt gtcaaagcaa aatcttgtat gggaatgcat 60
cctagttgag cctgaataag actgggggaa agaaaaaaaa 100
<210>273
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>273
caatcttcct gcttctgtaa gaaaagacag aaaaatgaac ggtcccctca tcagctgaac 60
caactgtttt tttatgttta acaaacattt cctgagggga 100
<210>274
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>274
gtatgcagta ctatctctgc atttaaatgt tcaataagat gtctgtgcaa actaatgaaa 60
aaaaactttg cattatgaat atgcagttca atgccataac 100
<210>275
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>275
ttataactac taggtgagca ggcaaattta ctcccatagc taaagtactg gtagtaactg 60
catcagatta agacatagta attttgagcc atttaacttt 100
<210>276
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>276
tctcagtttt gaatctctta cggaatatgc atacttctgt aacctattta aaaataccat 60
aaaattattg agttactccc agtaacatta gagaactctg 100
<210>277
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>277
ggtctatggc tctcatccat tttcagacac acagctggtc tttcaccatc tgaaagccat 60
tctgcaatct ttgaaacaca aaaatatgtg cattaaaatt 100
<210>278
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>278
ccagtcatca aaacgctgac tgcacaaggc ttttattggt gccactgtaa caatataaaa 60
tatttacttt gaacatcagt tcaattttaa aattcttttc 100
<210>279
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>279
tttcactgag ccaatgtgag agtccaattc attgtcatta gaataagcac tcccatgtat 60
attgtcagat attttaaata atctgcaaac aaaaaaaaga 100
<210>280
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>280
gaaatcaatg gagcaggagg gagaaaccaa tccaatgact tttcattgtc tggatggcta 60
tataaaatat aaaaatgtga gtatatatgt taataaagat 100
<210>281
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>281
cggcctcggc ttcgggctga gtgaccacgt cctgcagctg gcatttcctg aatggaaatt 60
cctgtgggaa caaatgggcc aaaagggaaa gaaaaaaaaa 100
<210>282
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>282
caccctcttt tctccttttc ccatccaatt catcttcttt tttcttctgg gaacacacgg 60
ataaatatgg aaaacgtgtg aggatgcacc ccaaaagaaa 100
<210>283
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>283
gtttcaaggt atctggtttg aagttcccac ctgaaatcac catccgctga atctacacca 60
aagcagatca aaatgtcacc tccaggcatc cattccctta 100
<210>284
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>284
tgagccatca agcctcatgt aggtatgctt cctgtaaacc atgtattcct gccaaccagg 60
ataaaaggac acagtaaatc tgatgcaaat tactgtattc 100
<210>285
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>285
ttctgcactt cggtcattgc agtaagaatc caatggcact gcggtaacct gcagttaaag 60
attcacatga gatggctctt tcacgttgtc catacacact 100
<210>286
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>286
cgtagctgat ggagcctgta ggaggctttc agagacagga aaagagctaa ccatctgaaa 60
caaaccacag cagacacgct tattcagtgc tccatggagg 100
<210>287
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>287
aaatggagac caagtttctt gccgttcaaa taactccggg tgattacaca cctaaaagga 60
agggaaatgg taagacaata ttaccacatg cagacatcat 100
<210>288
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>288
atggcttcaa aatcatgtgt aagcgagaaa gttgatctga aatgccggga ggcccccaaa 60
taatacatta tagtgatttc acaaaaacaa cagaaacaaa 100
<210>289
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>289
ccaacgaaca ctgtttgata aaataagtta caaattcatg ttagcaataa attgttcttt 60
aattatccta ctgagacccc atattgacaa aaatgccttc 100
<210>290
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>290
cccaaatgtt ctctctctgc aacagaaaaa cccagtcagc caactacagg aaaagggaag 60
gtatttaatt caaaattata attaatgaca agaacaatct 100
<210>291
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>291
taactctccc caaacccagt gccagacttg tttgctgccc gtagggcagc tgctcgactt 60
tcttttgcat cttcatcaaa tgaccttgtc tggaaagtaa 100
<210>292
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>292
cggcgggcac gaggcaaggt ttccttacag ttcttctggg cctgcaaggc agctcgacgc 60
acctccttca tgcactggtg agcaagctga aaacaacaac 100
<210>293
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>293
atattgctgc aactgaagca ggcaaagacc atgatgggcc tgttttctat agtacagctt 60
tcaaattacc tttactacag aaaactaagc cagaggaaat 100
<210>294
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>294
actggggcaa taatggatta ctgtcatcca gtccatcttc actgtcatca ctataaattg 60
aagaatgagt ccacaattac agctgaccca ataaaatata 100
<210>295
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>295
tttctacgtc cattacattg actgtgagtt ctgggcctga ggtggggcat cagggtaggg 60
ttgggggaca gtctttggca ttctgtcctg agccatcccc 100
<210>296
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>296
ctcaacggta ccctcagcaa ttccagggac cttgctttct tctcaggtcc caccttctct 60
accttctgac ccacctttgt tggtttctct ctgcagaaac 100
<210>297
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>297
agtggcagcc cagccaggac ggaagcgaaa atcgaattgt ttgggcactg atgaggtggg 60
tctggggagc agcagaaggg aactgggtga aaaggaaggg 100
<210>298
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>298
aagggcacca tctccttctt tgagattgat ggacgtaaga acaaggttag tgcttgagag 60
gcagtgaggc gctggggtga atcaaatgac agcttcttct 100
<210>299
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>299
tccctaccag cgccggggct acggcaagct gctgatcgag ttcagtgagt atgtgtgctg 60
cggccagggg gtagtggacc cactatcggt gcctcacagg 100
<210>300
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>300
caatctcatc aactactaca aggtagggag gcaggcaggg gagacaggtg tgtgggatgc 60
agagtgcagt cctctgtggg ctgaccacct gctgaaccca 100
<210>301
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>301
ggctctgccg gatgctggac gagcggctgc agcggcaccg aacatcgggc ggtgagcgcc 60
tcggaaaggg gtcggtgatc ccccacctcc cccaggtgga 100
<210>302
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>302
ggcactcagg agcccgagtg atactgctgg tgctctaccg ggagcacctg gtgagcactg 60
ggctacaccg ggaggcggaa ccatggcagt ggggtgggag 100
<210>303
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>303
caggagcagc ttcagcagag ctgtgaggag gagggcagag gagtggggaa aacagggagg 60
aggggatggg aaatgaggcc aaagccccgc cccaacccat 100
<210>304
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>304
aggtgaaggg ccgtggacag gctggcacca ggggcagggc ctggtgggta ggggatcgca 60
agctaacggc tggcttgtca gcagcaaacc ctggggagtt 100
<210>305
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>305
gcatggttcc gtccacaacc tcagccttcc tgagagcaca ctgctgcagg ctgtcaccaa 60
cactcaggtg agctgggagg gcagggccag gcaggcaggc 100
<210>306
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>306
caagaataag gtactgtctc tgcctagctt ctcagacatg gcctggccca gacccccact 60
gatccagccc tttcccgaac ccaggcccag tcggtgcgag 100
<210>307
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>307
gagcaccatt aagtgtgggc gtctacagag gtgagggcaa gagacggaac ctggagggag 60
tggcagggac tggggctgcc ctaggcccag ggcgtgaccc 100
<210>308
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>308
tgaggactaa ggataaagaa gagaagaaaa agacagagtt tctgtgagtg aggccagggc 60
ccttgggccc tgggagcagg aagtgagttg gaggggtaag 100
<210>309
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>309
cactcaaaat ccgttgccgg gctgacctgg tcagattgcc cctcaggatg gtgagtgcct 60
gaggcccatg ggagaaggac ccaggagctg cttctggaga 100
<210>310
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>310
cactggaagt ctcactgtct cgagtgagtg ggagagatgg ctctccacgc ccctcaccct 60
gtcctctgct gcctcttgtc tctcttgtca gttaatggag 100
<210>311
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>311
gcagctttga ccctgaaaca ggaaaggcca atgacggcgg agctggtgca cacctccatg 60
ttctgcaaac ctccggggaa gccacaaggg aaatgggcag 100
<210>312
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>312
ccccttcctt ctgcagggac acacaggagg tggccaccct cagccaggcc ctctccccat 60
tcaccacctc tcttccctct gtgatcccac agcccagtgc 100
<210>313
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>313
ttaagttgat caaccaggtt caatatgttt gtggaagacg caaagccggt ttctgagtca 60
ataatcagtt ccagctggaa gaaagagcag gtatcatgct 100
<210>314
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>314
ttatctcaat atacaaggac tccaagacac tgttaatgtt gttgatgaag tcctccaact 60
tatctacggt ggcattgcct ggaaataaac agaccaaaaa 100
<210>315
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>315
agcgccggtg gacatcagtc atgacgccca ttctccttgt gctgccctgc atgtgggaac 60
gaactgaaaa ggaagtaaat gacactagtc aaccccaagc 100
<210>316
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>316
ttggatcggc aactaaacca ttatgtaaag gctgttcaat ctacaataaa tcatgtaagt 60
ttataccact ccctggttat atatttggtt acaactcact 100
<210>317
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>317
gatataattg tgacccaaag tcagaccaac ttcacctgcc ccattacaaa ggtaccgctt 60
cctcctactt cccctgaaag aaacacgatt cacttcacag 100
<210>318
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>318
aattgagaac cataacaaga aaagacatcg tcattccgag taggaaaagc cacctgcctg 60
cagggacacc agcagcctac ctcctacccc agctgtctgt 100
<210>319
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>319
attcgtagtc gacggggtcg gtgtggggta tccgccggta ttccaggtaa cgctgtcgca 60
caaagtcctc agtaatgagt ttctttggat ctccgaaaat 100
<210>320
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>320
tctggctcct gggccccacg gcgggggcgg cctgggcccg gcgggcgccc tgaggcgagg 60
ggccctgcga cccctgcgag ccgcccggcc cgcgggacgt 100
<210>321
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>321
tgtgtgttat gttcaaatcc ctcattttct tcattaatac taacaggctc tgtttgaaag 60
acaaattttt caaatttatc aaaggttatt aaatcggaac 100
<210>322
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>322
cttctttctc tgttgcttct tgatgagatt cttccattct tcttaactga aaaaagtcat 60
tatcaagctg acagacctac ttactggcct attaacgttt 100
<210>323
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>323
tcaggactat cttcatcacg gatgagttct tcagcttcta gcggatttac acccatatgt 60
gtgagctaca aaaatgaagg aaaacaaaaa acccagttaa 100
<210>324
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>324
ccgcgcagag ccgcggcggg acctgggcga caaaggggac cgcgagcggg agcgggagcg 60
ggtgcgatcc cgatgcgggt cgcggccccg gccccggccc 100
<210>325
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>325
gagctgaaac acagggcttc gcaacgactc actctctttg gcacagggat gactcaggac 60
aataaagaga agcccctaat ttcggagaaa aataaatatc 100
<210>326
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>326
ttctttgcct ctgtaattaa aacagtatga aaagtcagta ctttgcacta aagcagtctc 60
taggtagccc ttctccgcat tgttgaactg cataatataa 100
<210>327
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>327
cgagattata cacatcaggc actggaacta tctgtaatac tggaacctaa ataaaacaaa 60
gcagccaaaa attatgcttg gttgtttcat ttttgtttaa 100
<210>328
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>328
actgtcgaat tgtttagtat cactggcaag acagactgag tctttcaaat gagcaagttg 60
gggtgtgcag caagttcgtc cagcaacttc tgtagatgct 100
<210>329
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>329
gacaagtcat tatcttcagt gggcccagcg ggagagctga ctttagttaa tgagagaagt 60
ttctgagagg ttcttgaact tggttgtcct gtgcatgtgc 100
<210>330
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>330
tgttccttta gtcttttccc agacaatctg agtgaatcag tgccaaagac acagtctctc 60
tcctgtgaaa taaatgtcct cttctgctgc ttctttcttc 100
<210>331
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>331
tgaaggcggg ctagtgtctt gctgtattcc cttttcaaga atgctaattt ctcctttaac 60
tggaagaaga aaaacaccaa caatactggg caagtggaaa 100
<210>332
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>332
gaagatgatg cccagaggaa cttcagtgtt tggatgagat ggggccgagg taatgatttt 60
tattgagatt ttatgagttg gcattcagaa agtcagaagc 100
<210>333
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>333
ttaataaagt tgatctgtaa tgttcaggcc atggaagaaa tgatgatgga aatgaagtat 60
aataccaaga aagccccact tggtaggact tcacattttc 100
<210>334
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>334
tactagaggt gagatatgta tgatttgaga agtattatat cccttatacc agtgtcctcc 60
cacattgatt ctatatctca tcttctcagg ctttgggaga 100
<210>335
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>335
ggagaaggat ggtgagaaag aagccttcag agaggacctt cataacaggt ctgagtctag 60
ctttgcgttt ggaaagacac tccttgcccg aaagtacagc 100
<210>336
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>336
actgctttgc ctctcgccta aagaatacag gactgctgct cttatcagag gtgagacagg 60
agtatgtctg tgatctctag tttattaatt ccagtttttt 100
<210>337
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>337
gtccgtatgc ggtacctttt aaaggttcag tttaatttcc ttcagctgtg gtgaatgttg 60
atattaaata aaccagagat ctgatcttca agcaagaaaa 100
<210>338
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>338
aattatttcc gacaaactct aaggaaaaga aaatgagttt ctcagtgacc gaacgctgaa 60
tgtgatacag ggcaatgacg gactcgctgg cggactccac 100
<210>339
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>339
aagagaaaag tacttctgcc tcagcatctc ggagaatgta gttctgctca tctggaaaac 60
agaacagcac atactctgaa aagtgtggac acgtgaagtg 100
<210>340
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>340
aggggtcaat tctggagtta gctttttagt gggaggtggt acgtcttcaa ttctgtggaa 60
gaaatacaca taaaatagta gtgatttcat atttacttac 100
<210>341
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>341
cgtatatttg gcacctgcca aataagccat taattttagg tcatctctgt cactatcaac 60
aaatccagtc acagaaataa tctaagaaaa aaagagaaaa 100
<210>342
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>342
catgtgcctg gattatctac acacaaagac ccgaccaatg cagtcatcaa tcactcagaa 60
tagaggagaa ttccaatttc aaataaggca gctattaaaa 100
<210>343
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>343
gctgaatctg ctgttgctgc tgctgctgga gcaggctgtg ctgctgctgg agctgggcaa 60
gctgctgctg ctggagctgg tgctgctgca gctgatggaa 100
<210>344
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>344
tgggacattg gcacacaagt taatcaaccc aggctccttt ccctgaggca gcctgcgttt 60
tgctgcagct aactgtggga cttcggccgg ggtccagttt 100
<210>345
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>345
gtaaccaaag cccacagggc acttctgtct tcagatgaca cctgacagaa agttagaaag 60
aaggctttta ccatacattt caatctgtgc atggctatat 100
<210>346
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>346
tgacctccct gaacatctcc tcaggaactt tgggcgcctg gtccgacatg atcgcggcgg 60
cccgggaggc tccgcggcgg cgcccggccc cgcccacccc 100
<210>347
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>347
ggaaagctaa cctcaccaag taagaaaaaa acattattta aggagacata aaggagtcga 60
aaataatact ccatgaggct aggaactggt gttttgaatt 100
<210>348
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>348
ccatagagag ggagactggt tttcagtgtt cagctggaat ttcacacaat aaggtgaagg 60
ctaatagtag atttcaaaga gaaacattga tatccttagt 100
<210>349
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>349
ttcactgaat cccagctcca gagtcatttt ggggagaaga atgggtaagt gattcatttt 60
ttttaaaatc ataaccttta tggagatgct atattcaaat 100
<210>350
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>350
gatgcattta ctgtcatcaa gaactgtaat acttctggaa tccagacaga atggtgagtt 60
cttttctagc tcatcttctc agaatagatg atgattctta 100
<210>351
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>351
ctggtaccgg tatgggatat gccagaacac atggactatc attttgcatt ggagttgcag 60
aaatcctttt tgcagcccca ctcttcaaac ccccaggttg 100
<210>352
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>352
ttgggcagaa catcccttgc agttttctct ttcgtgacaa tcctacttca tgaaaaagga 60
gcaaaactga tcagcatgtc gctaagtcat aggagaatgt 100
<210>353
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>353
gaattgatta atccctacaa agaaaataca gaaataatta attagaacca aagtctatcc 60
ttcacttcat tcactggctt aaatatggca aaatacacca 100
<210>354
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>354
cctcagttca agctgagagt agtcagcagc cagtattgaa ccacctgaag tagaagtgat 60
ttcagaaaag ctagtacaaa gtttctatat cacacaatcc 100
<210>355
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>355
tgatttcaaa atctcttggc acattctgaa tatttggttc tgtaaaggtt attcgtccta 60
aaatcaagca gaataaagac ataaaatcag gaaaaaaaat 100
<210>356
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>356
ccttggtttt tcatctctct atttgggggc aacttcaatt ccaaaaataa aacctgcaag 60
aaattaatgt ctctttagtc aagaatgaaa cttgcaagga 100
<210>357
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>357
ggagtggaag ctcatgagga agaaaactgg taactatgct atgaagagtc ggctcctgag 60
aatctggatc tagtaaccag catgccacct gaatgggata 100
<210>358
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>358
acaaccttta atgggaaatc catcatctct aataggaatt tcctgaggtg agctagctaa 60
gtaaaacaaa agtgaaacag ttaaaaatct ctaaaaataa 100
<210>359
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>359
gatactttat ctaaaatcct ttgcagtcct ggacttagat caaatgatgc ccctgaccat 60
atgaatgaat gattttgcct ggtcccagtt aattttgatt 100
<210>360
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>360
tcttcaaaat cacagaggac taaacctaag tcagaaacat gattattttt agttttgttt 60
gttgtataag tacctgtttt ttcttgtagt ctagaaatat 100
<210>361
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>361
tttcttgctc tgaaaatgga acatgtctga tgttcttgat tcccattctg gaaattacaa 60
ttaaaggaac ttgtatgctc tctactttta tttaagtctt 100
<210>362
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>362
aaattgggaa agtagtagtt ccatgttgtg ccagcccaga cggttggaaa atactgtaat 60
catccctaga acattcatag aatatttgtt gaattcaatt 100
<210>363
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>363
cattgaagtt ggcaacttct cccataaaca gaaaaatcga taccaatcaa tagtagtcca 60
atcctcaaac ataggtgtaa cctaaaagga agaagtatta 100
<210>364
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>364
ggcagcataa gtatgcatat cttgtgatgt acttgccact ccaccaacta ttatctgaaa 60
gaagatattt ggaaatttct gtagcttttt aagaaaacat 100
<210>365
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>365
acccgaatga gaccttgacg aaaggctcct tcaatgatat ccctctcctc aaaagtaaga 60
cctaaaaaaa ggaggttttt atatatttat tgatatataa 100
<210>366
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>366
aatcgtctca taacataaac taacaacatg gtcctcatct ccctaaaaca gaaagataaa 60
taaccacttt agtggtcctt tcaaaagatt ctcacatcag 100
<210>367
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>367
agataccctc ggtgagagtc tcccagcata tgtaattcat ccacaaccac cattcctaaa 60
aagattttcc agatactagg tttttacaga atgtcacatc 100
<210>368
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>368
cccagcactt gtaggagcta aaacatgatt attccacaag attataagaa aaaagtaata 60
ttcggtacaa ttcattaata aatgttttaa gccatgtgta 100
<210>369
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>369
tgctgtcacc gccgcttccc gagaacgaat ctgagcctga ttctgaacgc cgccgtttcc 60
cactccgacg cagaagattc atggcaaact cttctcggcc 100
<210>370
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>370
cctcctgctg ctggcactta aggtggcagt ccccggcttc tgcaccccca acctgggcga 60
cctcgggccg ggcctgtctt agtccgttcc gccctcatct 100
<210>371
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>371
gcctcatgat gggcccatgt gtgacctcct ctggtctgac ccagaaggtg agggcatgtg 60
ggcaggggca ggcagggaca gccaggaggg gttgggaaag 100
<210>372
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>372
agagacacgg ggcatcccct ccaagaagcc cgtggccgac tacttcctgt gaccccgccc 60
ggcccctgcc ccctccaacc cttctggccc tcgcaccact 100
<210>373
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>373
gcatgagatg cacagcaaac tggtccatgg gaaggcagtc atcctcaatg acagtctggg 60
gaagagaaac agctgctcta ggcttctctg tcaatgtagc 100
<210>374
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>374
tgggacgtaa atagtcataa acatctctgg gactatataa ctctagaagt aaaatcagct 60
gtctacaaaa aggaattaca aaaagacaat gtttaaaaat 100
<210>375
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>375
taactgcaag ctcgtggatg gagaatgcta gagttcgtcg aactttccac tgcagaaatg 60
aaaacacatt agtaagctaa ccacaaagta cgcagtactt 100
<210>376
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>376
gagtggagtc catattctgt taggaaaaaa taactactta aagagctgtc ctgtctgtac 60
atcttcattt aagctatctt actaagttac ttacctttca 100
<210>377
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>377
tcaggaagag gagtcctcca gaaatggaag gagttataca attcctgatc taagagagct 60
gaatcttgtg aagtggtgcc aacctctggt cgtaacatag 100
<210>378
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>378
gaacaaagct ggaaaaatct gggcagctat gtgaaaagga aagagtcttg ttagttggat 60
aaagatagca gatttcatca gttacttcgc tttcattttc 100
<210>379
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>379
gtctccacat caaatcgttc aatgagctcc tgctccaaca gagccagcaa agctgcctga 60
cttgttttcc tcacctagga gaaaaataac aacacaaaga 100
<210>380
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>380
tcatcatcgc agacttccct caaggtatcg agcaaactcc gggccaccat ttgtctacac 60
aaaaaaaaca aaattatgta tgttcaatgg catcaatagc 100
<210>381
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>381
agccaagact ggagaaacaa tgtgagttga aaacatgcat ttgtcgttat agaccagtgc 60
attattaaga attgctcagg aaagggaaaa aagtatattt 100
<210>382
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>382
tatattccct ttgatgaaat gaaggaaaga atactgaaaa ttgtcactgg atttaatggg 60
tatgcactta atactgtttt aaagatttaa tttttagcag 100
<210>383
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>383
caaatttgca gcaaaatgag gagaaaaatc acaggtttgt atgttttata tttaaaaaca 60
gtgtttttat taaaagttaa agttaacatt taatcagtac 100
<210>384
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>384
tgtaagtagt agttcttctg actgccgtga aacagaagaa ttagtaggat ccaattccag 60
taaaactgga gagattcttt cagaatcatc catggaaaat 100
<210>385
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>385
gatgcgacag aatcccactg agacgcttcg gagagtgttg ccaaaagtca gagtaagttg 60
gtatgaaata agattggagt ttcccatttt ttcccagtag 100
<210>386
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>386
cccagatgtt gaagtcagca caacaagaga aaaacccaaa tcctgataag acttgaggat 60
ttttcgaaaa ttaaatgcaa aatagttggg gcaaatgttg 100
<210>387
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>387
tttagttagt tgtaaaattt taggaaaatt gaccaacaaa tttgatgccc acacgtgagt 60
atttgtatgt aattttcggt ctactttatt acattcgttt 100
<210>388
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>388
cagcttttga aactttggtt aatctgcttg atatatttga tacaggtaaa tcatgtggct 60
acatctttgc ttctgaaaac agtgtttttc tacatgtttt 100
<210>389
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>389
cagattccac cccaaatcct agagcaggag aagaaatata tttcagtacg gaagaactgt 60
gcttataact ttccggtaat aaatatgtat ttatatttac 100
<210>390
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>390
agaaaataag gtaaacttca tctttcagaa acattctgag ttgtgtaaat attatcctac 60
tctacagggt gttttgaggc atcaatgaaa gaatgtttgg 100
<210>391
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>391
tcattcaaaa attaattgaa cgtaagtaat cattgtctac tattttgaaa aaggaaagta 60
aacaaactag ttggctttag ttatgtctaa cttatgtatc 100
<210>392
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>392
gatgttctgg ctattgtaaa aagagtaagt atcactcttg ccacaatgtt gcattttttg 60
cattaaggca gtaagaatct tagcttcact atttcagctt 100
<210>393
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>393
ccccatttct gttcctggac cctcttctgt caccccatcg acaagtaaga aataacttct 60
ttttatatct ttttgtaatt tttcttcctt gtatacaaat 100
<210>394
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>394
gagcagtgga agtaaagata cacaaccacg gaaggctacc ttgtaagtaa tcaagtgatg 60
tcagactgag tgtattatcc tcctcttttg cttaacacac 100
<210>395
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>395
aggagctaat aaatattaag ggaattagtg aagccaaagc tgataaaatt ctggtaagta 60
ctgcttactt aacctaggga ggcattagtg ggaatagtac 100
<210>396
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>396
ctgtcacctg ccaggtgagc tgttggggct atagctaatc aaataagcaa gcattacttc 60
attcctgcta tttgcaagca tctgttagga tggccataaa 100
<210>397
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>397
cagctccttt atcaagcatc agccatgatg gtagaatcta ggtatgtgtt cagtataaga 60
caccaaatat gttcttaaga gtccttccct gaatcttgta 100
<210>398
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>398
aaaacctatt ggaggaaata tcatcgccca tgcatcaaca accaggtaag gtgttgatgg 60
gatcagttct tcttttcgga atgtcatatt aactgtgaag 100
<210>399
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>399
tatgtatggt cagcagggcc tgtgccccaa agatgcaaac ggtatattta tattttatta 60
tgatttgatt atgaatgatt cctcatctca ttaaacatgt 100
<210>400
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>400
tctgcattta gtgctgaaag gtatgagatt ttattttcta ttataatgtt ttacttttgt 60
aacttatata cagcatgaaa catttacaca taggttaaca 100
<210>401
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>401
aatctcaaag aaagaaacaa gttcttggca agagaggcat cctccttgaa gtatttggct 60
gaggagtttt caatcccagt aagtttttct ttttttctct 100
<210>402
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>402
ttgattcaga gagaagacag gtgggtgctt tgacagtatt ctctgactat gaaggtcggg 60
gaatgagata taccaagcaa tctgagcgtc ttctgaaaat 100
<210>403
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>403
atattcttgg gggtggagtg cccttaatga aaacaacaga aatttgtggt gcaccaggtg 60
ttggaaaaac acaattatgg taaaataaag tgttctcctt 100
<210>404
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>404
ttactggcac aagtttatct tcttccagat ttcctttcag aacactcaaa ggtatgagtc 60
agactactga aatgtaacta accaagtatt ttttgaggtg 100
<210>405
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>405
aaggttcctt gagcacccgg aaacggtcac gagacccaga ggaagaatta taacccagaa 60
acaaatctca aagtgtacaa atttattgat gttgtgaaat 100
<210>406
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>406
gtgctgggca caaagctcca ggagcgtccg agggcaggtt tgagcctccc gctgtccctg 60
tctcgagtta tgtggttggt cacctgcagc agaaacagac 100
<210>407
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>407
tccggagagc ttctgcctga agcggtggaa aagaaaagca aggactttgg ataagaggga 60
gtaggggggt caaggtaagg ctgagaagga agaggcacct 100
<210>408
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>408
ttgccgctac ctgggcctcc tacaatttca gtcacttctc cagtatagag accagcatca 60
agcagtttat caagactgat ggcagaagag aagaaaatca 100
<210>409
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>409
gcaaccaggg cctgccaaag ggccccagac tgctcagcaa caaattgccc gtagaagctg 60
gcatcccagg gtgtcattca ccctacttct caatatctga 100
<210>410
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>410
ggaggggccg gaggcgctgc tacggttcac agaggagaga aaggacaagt catcatcaca 60
tcactgcatg ggatttcaga gcgtgggtcc actcccctct 100
<210>411
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>411
tccacagacg gttcaagatc ttgtctcttc gctttagtta aatccacttc aagaggcaac 60
tagaaggaaa gaagaaaaac aaattccttc aactggctct 100
<210>412
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>412
tatgaaccat cctgggggca gaaaaggagt ttgaactcaa acacgagagc ggcaggcgtg 60
ccacaactcc cctgttcctc aagcagcagg gttcaaagcc 100
<210>413
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>413
tcattggcca gattaattac ccgatgaccc tcaatgtagc acacctagaa aaacaaatgt 60
ttttaaggaa aagctgtgtt aattccttac cacacttaaa 100
<210>414
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>414
caaagacctt gcttactgat ctgcctttga tagatctttt cttctattgt acctaaagag 60
aaaacattaa ttagatttcc ttcagatctt tatgttcatt 100
<210>415
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>415
cctttgtata gactcttttc ttcattttta tcacaagttg agctacattc cttttcctat 60
tcaaaatgat gattgttaat gctggctcaa gtttgttttt 100
<210>416
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>416
ttacaaaatc aattaatgca aaaaattctt gcagatcattctgaattgga gtacctaaag 60
agagacaaaa atgagtaaaa ggtcaagttt gtgttttaca 100
<210>417
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>417
cctaaaccca tttcatcagc aagaatagct ccacatctgc cattcattct gttagaaata 60
attttacttc ctttaatcac aatagaaaaa tattttatgc 100
<210>418
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>418
ctgaagtcat ctggagagat tacacccatg acttctattt cttttccaca aatcatcagt 60
gtttggccct cttcaatctt ttcaagctct ttgaatttat 100
<210>419
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>419
tagtactttc ttcactaggc agatttaaac tgcatattct aagatcattt tgtgacggga 60
gaaaggtgtt attaattgca acaccctaaa agaaacaaat 100
<210>420
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>420
gtcagctaac caatcaacca ccttgtctgg acagcagtca gcatgtaagc cagaactgca 60
acctgcatgt gtatgtctgt gcccagtcac tcagcctagg 100
<210>421
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>421
ggcctcatca gagagaggta aatgagggtg aggggaacga ggtatgggct atgggctgag 60
cctgggagac taccatccct gggacagcag cagcgtaggt 100
<210>422
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>422
tcatatgtga cgaggtactt gactctcagc agtctgggtg gtaggagaaa atctgtcaaa 60
atctcttcac tgagtattgc cttccaaggg ctgtgctagt 100
<210>423
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>423
cagcattgtg aataggtaat gaccttaagc gaagtcatta gaattgcctc ccaaaccatc 60
catggccaat cctttggggg ctttgcctct ctagagccct 100
<210>424
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>424
cttggacctc ttccctcctg gttacagctc taaggccctg gagccccagc tgtcaggtga 60
cccttttcct accagtattt gggcttctct aggaggaggg 100
<210>425
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>425
ggcccgggtc tggcgagatg gtcaaaagaa gacttgctat atctaccgcc tgctgtctgt 60
aaggatggtg atagtagtca tagtaggggt gggtcatgga 100
<210>426
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>426
atcaccttcg tcttccacca gcgttctcat gaggagcagc ggggcctccg ctgataacca 60
gctggtctgg gtgtagctct tagaggaagg agatagggaa 100
<210>427
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>427
aagttttaga agatcggtga gtctggcact taggtcttga gtaagaagtg atttcctcca 60
taataagaag tacattgact ttcatttgta attagttttg 100
<210>428
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>428
tgtccgatac atagaacaaa cacatactaa attggagcac tctgtgtgtg caaatggtaa 60
gagttggagt tgtattttag ttctctggtt ttgtaaacca 100
<210>429
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>429
gtgttcaaga agaatctgta agtaattgtt tagtttggca ataacatgaa ttaattttat 60
gtctttttag gtcagttctt agagaattaa gcaagagaat 100
<210>430
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>430
gtatatctag attagaaaaa actagatcaa aatctgaaga tagtgccctt ttcacacatc 60
acagtcttgg gtctgaagtg aacaagatca ttatccagtc 100
<210>431
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>431
cagttcttca gttaaatcca tgtagaactg gtaaaataaa gtctctacaa aacaaccaag 60
gtgtgtacac cataaacagg atctccactt ttttaatgac 100
<210>432
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>432
aaagttcaag taagatctgt ttttgttttg ttattttttt taataacgtt gtgtgaagtt 60
tgtagttgta ccattgcact gattagctca agtgacctct 100
<210>433
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>433
ggagagaaga aaactgcttg ggcacacgtg taaggaatgt gaaattgtaa gtactaatgt 60
agatactaat ttttttttaa gtacggcttt atagataata 100
<210>434
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>434
ttacaacgca atattttctc caaaaggcaa ggagcagaag acatagacgt tgaaacagaa 60
acagaaggat gaaggacagt tttttccttc ttagttattt 100
<210>435
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>435
tccagggaga gcaagggacc tgcaaaatgt gcgctagtta gggtggcagg cagaacgcac 60
attacggtca ttcattccaa aatgttgccg tttttgctaa 100
<210>436
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>436
ccttttcata aggaatgata atggtcctaa atccaaccaa aacaaatgga tttaaggtgc 60
ctatttgaat gataattgct atatgtttaa aaaataaaaa 100
<210>437
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>437
ctttcttcaa ggtcagaaag ctcctgaagc tgtaaggata tttaatgtaa ggtccttcct 60
ggggtcttaa tgtaggcttc aaaatgtttt gttattaaaa 100
<210>438
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>438
actgagaaaa tgctgtgaac agagaatgct gtgggggtgg gacaagtaaa tgcatgaaat 60
ctcacgccca gaaaacaaaa ccgcacatgc cctcatattc 100
<210>439
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>439
gtaccaactc cgccatcctc ccttcaccgc acctgcgcgg ctaaccctcg ccccagcatt 60
gcgcctgcgc aaggcacgcc cgcgcttttt taaaaagagg 100
<210>440
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>440
tattttcaaa tacgaaatgg tagcataagc tgtaaaactg tagtcttctc tgcagaaaat 60
aaaggccaac aataagaaag cttttgaagg aatcacggaa 100
<210>441
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>441
acacacgacg acatctgcaa acacatttaa agatacaaat tattaaagga tataataaag 60
ttttaagaat ctctatttca gcttacatag ttatatactg 100
<210>442
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>442
ctactacctg aaatatttta acattttatc agttaattaa atcaagttta aatattttaa 60
aggtaaatta ggcttctatg gaagatatag acctaacctg 100
<210>443
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>443
ctgccgaacc taaaaaaaac ttaacttatt aaaaagtaaa tgaatgagta ccatccaagt 60
ggctgtctat gtacaacttt caggatgcag ttctttcatg 100
<210>444
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>444
tttctctgga gtcacataaa tcagctttaa ctcggagttt ttatttacca tttcagcatg 60
aacccattta acatgctcct attaaaagaa aaaaatagac 100
<210>445
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>445
ttttccagtt taaagacatt ttgcagaata tctttaactt taccagacca tggaaaatct 60
aggaaaagaa agttaagaat cagacaaaca tgtaagacta 100
<210>446
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>446
tcagggacag gaactggcgg atgtcgcagc ggacctggtc ctcccaatcc tggagctgtg 60
tggacaggca catcaggctt cctctgagct cccgtggcac 100
<210>447
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>447
ctgccggaaa gcatgtcaga tgcaggcagg cagcgtccag ggcggtgtgg ggtggggaga 60
ggcgcacctg tcctgggctc gtggtcccac tgcagctggc 100
<210>448
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>448
acagcaaagt ctcgatggct gggggcagag cagggctcag cggacgcggg gacagcccct 60
ccacacccct gtggcttacc ccaggttcct caccctcctc 100
<210>449
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>449
acccggcaac tggccctgca tgaaggctcg ctgtacccgc cgccgttccc ggctgcacat 60
gcccgcgtgg taggcctcgg ctgtggtttt gggggcacga 100
<210>450
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>450
ccggttgcag taaatgataa tggaatcgag gttttgaaaa cgtttgcctt gcagcagcgt 60
caacagtgcc tgatgaggag cggttggcgt gggcagtggg 100
<210>451
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>451
acgtgtacct gggctgcccg aatctgaagg cagcaagatc agaggcacag cccaggtgcc 60
cgcccgctgc ctccctcacc cctaggccca tgaggccccc 100
<210>452
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>452
taggcagcac cagcagcgtg gagatgcctg gatggggcgg gagtcagcag cagggttctg 60
cagcctggcc tcagcccagc ctcagccctg gcagccacgc 100
<210>453
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>453
gaaggaacca gtggctcagg cccaacagca tctgtgtctt cctcacttgc tggggcaggc 60
aggagagggt agaatgggag ctccaggtcg ggcaagacaa 100
<210>454
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>454
tgggctgctg cagggctgag gtggctgtgc ctgtacaggt tcccctggag ggtcttcctc 60
aactgctaca gccccagccc cctccgatgg gggtccagct 100
<210>455
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>455
cccagcagcg gggctctggc gcctgcagga gacaacaggg gcacaggcca gaaaaggctg 60
ttgtggcggc agaagcgctc ccagccaccc ctcccgcacc 100
<210>456
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>456
tcgtcctgta aagggaacgc gtcagccgcg ggccgcgccc tcagcccctc ggcccctggg 60
cagcccgcac ctggctcggt cgccgcccgc gctgccgtcg 100
<210>457
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>457
ggtcttctga gtcagcaagt gtgagaggtg gcgggcaaag cctttaaaca actcctggaa 60
gggagaggga gaagagaagc agagagcagg acccttggtg 100
<210>458
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>458
caggtgctgg tagagggaag agcaggcatc acagcactgg gtcctggtgg ctccctcatc 60
ccaggaggcc taggagagcc catctcaggg gtgagctctg 100
<210>459
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>459
ggctctgtgg atatcggcca cctgggcagg gataggcagg gaggctgtgg cacccgccag 60
ctcctcctga catgccccac ctagccctcc ttttctacta 100
<210>460
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>460
cctccagaag ccgcaggcag tgctcccgtg cctggcacag gacagtgaga ctggtcgcat 60
ggcctcgcgg ctgggggagg gccccggcgg gtggctgagg 100
<210>461
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>461
tctggcagtg gtcgcagcct ttggcgcagg caggcagcgc atccccgaag tacttggcaa 60
tggcggcatg gcggcacctg gagcaggcag caccgcagag 100
<210>462
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>462
aggcttccca tccctgccag cccggccaga ctcctggtag tacccagcca tagacttggc 60
aatattccaa tgggcgacaa acctgtagga tccaaaggga 100
<210>463
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>463
gctgttcaca agcctctcta gtcctgcagt acacaatgcc gcagccagat aactgaatgg 60
ggagatgcag gaagaaaata agcatagcaa gaacagaaca 100
<210>464
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>464
attttcctgc ccctgtgggc atgcacacaa agacgtcctt gttacctgaa aaaatacaag 60
acaacaatat ctcagtgctt cctgcctttt cattgaagtt 100
<210>465
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>465
atttggagca tcctctttta cccgatggca ttttagaaat tccaaaagga gaattaaatg 60
gattttatgc tctgcagatt aattccttgg ttgatgtaag 100
<210>466
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>466
acattcccag agttacagat gttctagatc ctgcattagg tccttctgat gaagaactct 60
tggcaagtct tgatgaaaat gatgagctta cagcaaataa 100
<210>467
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>467
gacaaatcat ttggttgtcc atctgttaga gaccaaaaca ggagtatttt ttcagttcat 60
tgtaatgtac ccttagccca tgattttaca aataaagaaa 100
<210>468
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>468
atgaaatact tactagcttg atagggttct cagtaccaga aatgaaacag tcaaaaaagg 60
atcctcttca ataccaaaag ttcctggaag ggttgcagaa 100
<210>469
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>469
gcagggcagg gtagtgatgg cggaccgcgg cgaggctcgg ctgagggacc cgagcgggga 60
cttctcggtc cgcggcctgg agcgggtgcc gcgcgggcgg 100
<210>470
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>470
gacctttctg ataatcccat ccatgaaagt atgtgggaac tggaggtaga agatttacac 60
aggaatattc cttagagtat gttggaactg tcgttaaaaa 100
<210>471
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>471
gggccttgtc atcctggggc aacgctgctg ctgctgctgg agaccccata ggtgacccta 60
gttcccaggc ctctcctggc ctcctgtggg gatggttggc 100
<210>472
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>472
cagctgtgca tccatcctga ggtgaagaaa caagagagta accatctaca ggtaggctcc 60
tgggctcccg ctccggctca gtgtccgaca ggcgagtgct 100
<210>473
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>473
cgacatcata ttcatgccgt acaattactt gttggatgcc aaggtggggg ctcagtcctg 60
tagctgacga ctcctgatgt ccaggggtgt ccctgggctt 100
<210>474
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>474
aagacattgc aaagctgaag agtaagtgtt gccctccccg cctccttgca gctgggtggg 60
gcctcctcct tgcgaggagg tgggtgacac ctcctcgacc 100
<210>475
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>475
gaaagcgagg tacagacctg ggcccacacg ctccccgccc gcccgggtgc agtgcccggc 60
accaccatgc cacaggctag gcacatgccc agccgtggat 100
<210>476
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>476
gtgggggtcg tccccagagg ccccgatgga gcccagttga gctccgcgtt tgacagacgg 60
tgagggcctg tccctgggcc ctgctggggt gggaggtggg 100
<210>477
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>477
gtgagttaca gcagggtggg gctggggtaa ggcggtctgg tgactgagcc cccgccccgt 60
ggccaaggga gcccccgtga ccgagccgcc tcgccccaca 100
<210>478
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>478
ttgccgagcg aactgtgagt tcctgcccag ggaggggatg agggtgttgt ccccagagga 60
gccagaaatg ggtccaccca cccccatggt tctgcagatg 100
<210>479
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>479
cggggagccc tggcgaggag caggtacagt tccagggcct tgggatggac acagaccctc 60
tgtctcctga ggccaacccg accccgccca tctggcctca 100
<210>480
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>480
gtctcggccc cctctttgct gaggacccca agaagcacaa cctgctccaa ggtgccctgg 60
cttgcagagg ccacccaccc tgagggcagt gctgccgccg 100
<210>481
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>481
agagcacctg aaccagggca ggccccacct gtcgcccagg ccacccccaa caggtagctg 60
actcctgaac cgtgtgcagc ctacgacttg gtgggtccct 100
<210>482
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>482
ctgtgcttac ccacagggct ccccaaccag gtagggcacc tgcctggctg ctcctggcag 60
cgccccaacc gcacgcagcc ctgggagtga gcagcaaagc 100
<210>483
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>483
agcagagcgt catgcaggtc ttctggccag agccccagtg agtgcccacg gaggccccca 60
gcacacccaa cgtggcttga tcacctgcct gtccagctct 100
<210>484
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>484
ctaagccagc ccagtctaaa aatagaaaca gatgctgtct aaatatttct catacattat 60
ttccacaatt acaaaaactt tgaaaagctt gctactgaac 100
<210>485
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>485
gtaaggaagg agaccgggca gcggcggccg ggtgagggct tgggttccgc ccctcgctcc 60
gagccgccct gatgcccctg tacgccgccc gcagtttgag 100
<210>486
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>486
agaggggccc ttgtgttgcc cccaccctgg ctgcctgcta agggcccatg tgatctgcct 60
ggcagaggag tttcttcagg aagaaccagg gcagcttctg 100
<210>487
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>487
cactgctcgt cgggtccagc agcacaatgg gggccgcaaa aaaggcgggg cctggcggac 60
cagcgggcga gggccctggt gagaggggga ggccttctgt 100
<210>488
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>488
ccgccgcacg tgcctctggc cttcgggcct ccaccgcccc aggccccggc cccaaggcgc 60
cgcgcaggtc cctttgatga cgcggagcct gagccagacc 100
<210>489
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>489
agaagaggtg agtgggcttt ggtggcgggc tccctacccc actccctgcc ctgggctgcc 60
tgtgaccaca ctgcttgcct ctgcaggcac actggacaga 100
<210>490
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>490
gtaaggaagg agaccgggca gcggcggccg ggtgagggct tgggttccgc ccctcgctcc 60
gagccgccct gatgcccctg tacgccgccc gcagtttgag 100
<210>491
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>491
agaggggccc ttgtgttgcc cccaccctgg ctgcctgcta agggcccatg tgatctgcct 60
ggcagaggag tttcttcagg aagaaccagg gcagcttctg 100
<210>492
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>492
cactgctcgt cgggtccagc agcacaatgg gggccgcaaa aaaggcgggg cctggcggac 60
cagcgggcga gggccctggt gagaggggga ggccttctgt 100
<210>493
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>493
ccgccgcacg tgcctctggc cttcgggcct ccaccgcccc aggccccggc cccaaggcgc 60
cgcgcaggtc cctttgatga cgcggagcct gagccagacc 100
<210>494
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>494
agaagaggtg agtgggcttt ggtggcgggc tccctacccc actccctgcc ctgggctgcc 60
tgtgaccaca ctgcttgcct ctgcaggcac actggacaga 100
<210>495
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>495
ggtcccttgg gcctatgggc cccaggtcct gtggtggcct cctgctgggc atggacccct 60
gcccttgaag gcttgtaggt ctgggaggcg agggtctggc 100
<210>496
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>496
tagtgcagct tcctcggatg ggggtggtgt ccaggagtcc cggggagcga cagtcacggc 60
tgtcggcggc ctcgttcatc ctgcggctgg ggctgctggt 100
<210>497
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>497
agaggatgac ctcatcttct tcgttcagtt tggatgaaga tttctgagat ctggagctcg 60
aatggtcagg atttgactgg gttaggtcaa tagacggaga 100
<210>498
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>498
cccacattga cctcaagagt tcctggaaat tctcggccct gctttcgcaa ttctctgctt 60
ccttctcctc ccatggtttg ccctctgagt cagtggcaat 100
<210>499
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>499
accatggcgc caaagtcagc aaccagcagc ccgagggaga gctgaagcag gaggagagga 60
agagccgtca cctccccacc tctgctctgc taactgggca 100
<210>500
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>500
gggtgccgtg gagagcgggt gaccttcgct cgctcagctc tgagtgctca ggtggcacca 60
gaggcggcac gggctcctgc ataaggccct gaaagaagcc 100
<210>501
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>501
cttgctggtg ggtcctctcc gtttcagacc tctactgtga tcactgaagc tagaaaacag 60
ccaaagagaa aagttactgg ggctagagga gtgcacgctt 100
<210>502
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>502
cctacccgtg caaactcctg ctgcagggtc aaggccaccg cagcgtcgct ctctggggca 60
gggggcccaa gcccatacac tgtggagaag caccaaagat 100
<210>503
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>503
gggcctggct gccagacgga ggtttcttct ctgcagggcc ttgaagggtt ttggtcttgg 60
tagcagtttg tttggtcctt ttcaatttgc ttcttatctg 100
<210>504
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>504
aaattgctcc aatacagttc aagagaaaac attcaacaaa gatacagtga ttatagtgta 60
agctgattaa tagatatatt gtatttgcat gtgtgtgaga 100
<210>505
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>505
gaagatgaat ttaatgatga tcaatctata aaaaagacaa gactggatca tgtaagttta 60
catttgaatt acagtatcaa aattggctgc ttaaggttag 100
<210>506
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>506
aagaccaagg taattattct gggtcataga aaatacatac ttctcattat agtctgtatt 60
ttagtatata agaaaatggt atataagaaa agctattagc 100
<210>507
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>507
aaataaattg accaaacaag ttaccatgct tactggaaat ggaggtggat ggaacataga 60
acaaccttcc attctaaacc aaaggtaatc tttgttgaat 100
<210>508
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>508
ttgttccagc ttcaactata ggtttgtaat actggagaca actgtattta actttatcat 60
gttttatata ggtagaagag agtgtgtgta agggtgtgtg 100
<210>509
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>509
gatttaacat tcatagtaga tatgaagatt acaatgtaat tgtgaccacg taagtattga 60
gaaatttggg gaggatggat atttatgcag cccccaaatt 100
<210>510
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>510
acatgtttag tagtagcacc agtgaaatac gaagaatgtt ttcctctaag acagtaagca 60
taaatgcata ttttctccca aatatgttat ttgtgtttta 100
<210>511
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>511
caggagcaac tctatttggg tcttttcaac agattgaaaa aatctatcaa taacttgggt 60
ataatctgat ttttactctt ttatgtgaaa aagaatcttg 100
<210>512
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>512
aaaatttcga gttttaggat gcatcttgtc tgaattgaaa cagaaggtat taaaaaagaa 60
tggcgtttct tttgtatttt ctcaattact ttaacttctt 100
<210>513
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>513
acaaacaagc agaagataga tgccatagag taggccagac taagtaagtg tttttagttg 60
gaatgtattt ttaattcagt gccactttaa tttgtgtaga 100
<210>514
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>514
tgccatttgc cttggtttag ctggaaaacc tgctttcatg ggacgagcag ataaggtgag 60
ttaatataat tactttttaa gaaatttcaa acctattaat 100
<210>515
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>515
gaaagaaaaa cagctcgagg tactttaaat agacaactca tttgtattgt ttcttattga 60
tttctgtatc tcagaacatg ggagagagag tagtagtatt 100
<210>516
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>516
cgaattgaag aaatggaaaa cgagcgtcac aatttggagg ctcgaatcaa agaaaaggta 60
ctttttggtt tcaattttgg attatctgaa ttttatttta 100
<210>517
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>517
acgtatgatg ctgttttatg gctaagaaat aacagagaca aatttaaaca aagagtctgt 60
gagcccataa tgctcacggt aagaaacttt gttcatcttg 100
<210>518
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>518
gagttcatat gcagacaaag caccttcaag atctttgaat gaacttaagt aagtcttgaa 60
aatactaaga tttatttaaa tattgaatta ttcaacattt 100
<210>519
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>519
ggttggttaa tttgatctga atgttagaaa agaatattaa ctggatttct aatagtttca 60
ctggaacagt tttttaaata tgttaacaat ttttaaatat 100
<210>520
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>520
tgttcaaaaa gcgaaacttg ttaccgaatt aacaaaccta ataaaggtaa ggtattgttt 60
taattttagt cattttttaa aggaaatagc gggcagataa 100
<210>521
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>521
tatgtaacct gggtgcagag cagactcttc ctcaagaata ccagacagta agtataaaaa 60
gtatataatg ctacaattgc catatttaac atttttaaga 100
<210>522
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>522
aactaaaggg aaagaaagtt gaactagatc aatacaggga aaacatttca caggtaattt 60
tttagttttt aatcttttta tcatgtgatt attaatgaaa 100
<210>523
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>523
caggtatggt gattgttctg ttacttggat cttcttatat cctgtaacag gaataaatgt 60
aatcattatt gttgccattg tttagggaat ggacccaatc 100
<210>524
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>524
tacattcact caaccttctt aataaaagta aagagaggga acttgggaat tttttttgtt 60
aaattctgtt tataagtatg gctcaactga ataaaaggag 100
<210>525
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>525
ctcctattaa ccatatcctg aaaaacaaag gcaaaagtta ggattgctat tgtgtaagtc 60
acatttctgt aaaagcagca ttctgtgggc tgatacaagc 100
<210>526
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>526
aaaattcatt tttccacaat aggcccgctg agatagtaag ttgtcaaagt ataatttgca 60
tcgtaaagtc aaacaccttg aaatttaaaa attaggcaca 100
<210>527
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>527
agatttttct acttctatac gctctgggca gatttgtctt gcttgtgaca ttttctccta 60
agaaagtaga tgtttatatc tttaaaactc ttctttgata 100
<210>528
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>528
ttaagatgct ccatattttc tttttgttgc tccatatgtt cctcaaccat tttcattttg 60
cttttatttt cctgagcttc atcttcctga taaaatttaa 100
<210>529
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>529
ctgaagattt aatatctggg ccgtcttatt ttcaacctca ttctccaagt cacttgatag 60
gaaaggagaa gatagaaaat actttaaatc tttttctcaa 100
<210>530
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>530
atctatttct aaagctgtca gaactgttgg aaagtctgga tgataagcag ctctaggaga 60
caaaaataca gaaggattaa cttagcaaat tttaattata 100
<210>531
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>531
cttttgagtc gatcagtttt actatctttc aattctttca gttgcctctg attgtagctc 60
agtgcatgct tcacatctaa ttcttctctc ctaaaaaaca 100
<210>532
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>532
gttatataaa acctaaccaa aaaaattaga cacactttac aaaaatgaaa acacaaatat 60
cccattcacg tccacttttg tcaaatcttc agaggctgaa 100
<210>533
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>533
ctccaatttt gatattatct ctgatggcat tcaattgttt ttcaatttca ttgacctaag 60
aaagaaatta tatagcaaat tgatcagatt aacaataata 100
<210>534
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>534
tcaagttgcg ttgctttcat gaagaactat tatcaataac aaaaaaaaac gcaattagga 60
gtttgtaaac ttccatagaa ctgttaaaaa gaaaaagaaa 100
<210>535
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>535
ttccaccaag accgactatg agagctgtga gtactgcact cttcccacct aaagaaacag 60
atgttgaaga aatcacattc attaaaagaa aaaggaaaaa 100
<210>536
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>536
aaattttctt cctttctttt ggccatcagg tctgaacaaa tatttatagt aacaatgagg 60
taaatcaagt tccatatata ttaaactcaa caaaataaat 100
<210>537
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>537
ataatcacaa gcttggcaag aaatgaagca cctgcattca cgatagacaa cagatcaagc 60
tgggaaaaca taaagtgggc attttgcatt tttatttttt 100
<210>538
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>538
tcgtcgacaa tattatcaat gacatttctt gcatgttaaa agtgtcaagg aggagtctac 60
atatagtaag tagtacctaa tacaaaacat tttattttaa 100
<210>539
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>539
tgtctccttg catcatgatt acggtatatt atctaacttt actacaattc caagactaat 60
gtatactgtt atggtgattg gtaagaggat ttacaatatt 100
<210>540
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>540
tgccaaccat tttggagaaa agaagtatgt ttccttttac aactcaagtg ttgcatgttt 60
aattgtcttt tactttagta gtggctattc acatcgtatt 100
<210>541
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>541
tcctcttcct gacacgaagg cctcttcaaa gcatgccccg cgggaccgga acgactctag 60
acccaatgcg actccaggta accgtggggg tgggagcaga 100
<210>542
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>542
acttcagcca ccggcttggg cctggccggg attgtgggct catggtggcc ctccagaccc 60
aggaggaagc aggtagtggg gacgtcctgc acctggcact 100
<210>543
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>543
ccaaacccac ctaggtggat ccctgggcct ggtgggcatc ccagggaggc gcccaggggt 60
ctatatgcca aggcaggagg ccctcctgct cccggattgg 100
<210>544
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>544
gctgaggtag aacctgggga caaagtgcca tgtcagatga taggagtgca ggagatgctc 60
tccgctgttg gccatcagct aaggcagcct ttcctcagct 100
<210>545
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>545
ggaatcataa cctgcaggga gagagtcgag ctcagtgagg gcccacctga gaccctcagg 60
gacttgggcg acaccaccca ctacccactg caagctctgt 100
<210>546
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>546
gggctggtct ccccgtccac catctccacg gtgctgggct gaaagtggga tccttgctca 60
tagacaggga ggatctacag ggagcggcag gtgcaacagt 100
<210>547
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>547
tggatcgggg gtctgctgtt ccaccaacac cgtcaggttt aagtctctgg gaaaaatgtt 60
tgtttctgtt cggggatagc tgatgtacct aaaaccaagg 100
<210>548
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>548
gatgcctttt ccagttgaat tctacgatac catctttgtg gtcatgagtt actatattaa 60
ggagagacaa acagaaaggc tgtgtctcag agacatgtca 100
<210>549
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>549
ggcatggggt gtgatctcag agaatcgggc tcgcaacacc tgcagattgg gctttactgc 60
agaacacaag gtgttaccaa ggtcaaagtc aaatcacact 100
<210>550
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>550
tgaagtcatt accatggtaa cattctgaat gatgacaaaa ttcaaaattc agttagtctt 60
tttgcagtta ttattctgcc aaatcattaa aatgagaggg 100
<210>551
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>551
gctcgaagtc atccagaggg cagcggagct ccttgtagag cttgatggtg ccgttctggg 60
ggagcgtgta ggtctcatcg cagtgggagc agtgcaggcg 100
<210>552
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>552
ttctccactg cactgcggat ggtggggagc accagctctg catctgcaag tgggcagggg 60
gcagatattg ccctgagcac tcgcacccag ggccatgcca 100
<210>553
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>553
caagtgggca ggctctcctc caggggcacg ctctgccagg gcatgacctc cgtgaagcct 60
ggagagatat gcgccgccac tcagggtccc cagcctgggt 100
<210>554
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>554
ttctgctaac aaccgcttca cctgagggag agaagacaga gcagagtctg tggctgggct 60
cggctgcctg cctgggtttg gctgacgtac cagacctggt 100
<210>555
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>555
ccaggggcct ctgcttggcc ttttctttcc tgcttgtggc ctccacctgg aagacaggac 60
agtcaatgat ttggggaagg aggaagacac caccatagag 100
<210>556
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>556
tggagtctga cacggcccaa aggagatgag agagctgtct aaatcaccgt atttcccctg 60
gaaatattta gtctgaaacc tggagggagt ggaaagatat 100
<210>557
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>557
gagcttggct gaacagttct gcggggtcca ctttgtccca tttgttgtat tttcctacaa 60
accagtcaca gtgacattga gtcacaggag ctcagcctcc 100
<210>558
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>558
ggagacagga gtccacatga cactgatact ctctaaatgg gaccagggat ttacagaggt 60
aacacttctc attcctatcc aataagaaaa aaggaagaaa 100
<210>559
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>559
tggagagact tctctttcaa ggtcaaaagt ttcagaggaa cttagctggg tctctgtaat 60
ttaaaaaagt taaaatccca ttaattttca ttttgtgttt 100
<210>560
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>560
tttccctgac tgatatgtga gcctttaaac agtgatggag gtaccagctg ccatatgcct 60
gtaggtattt gagaaaaagt agggctaagg acaaactttt 100
<210>561
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>561
gttcacctcc ctagcttcct gctcactcat tttgagagcc agagcaaact gttcttcttc 60
tgtcatctct gtaagaggat agggaaaaga gaaggaggaa 100
<210>562
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>562
gtagtttcca catccttctt ctccaggttc cgagattcgg agacttcttt aacttttttc 60
tttctccgtg gcatcctttt gtctagaata aaaggacaat 100
<210>563
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>563
atccattgtg tatagttacg atgctagtga ttgctctttc ctgtcagaag atattaggta 60
agtgatttga atttcctgat tttatttgaa tttggaccct 100
<210>564
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>564
gagatcagtg gaaacttcta cgtgactttg atatcaaatt gaagaatttt gtggagttga 60
cagatgttgc caataaaaag gtaaaagcaa tatatatata 100
<210>565
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>565
ttcaatctct gaggaagtga tggatctggc taagcatctt cctcatgctt tcagtaaatt 60
ggaaaaccca cggaggttaa atattacctt tttttttttt 100
<210>566
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>566
taattgagag tgatgaagat ttagaaatgg agatgcttaa ggtatgttta caattataaa 60
aatattactt caagttcttt ccaaaggaca tttaattaag 100
<210>567
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>567
agaagaagaa gatgatgaaa atgaagctaa tgaaggggaa gaagatgatg ataagggtaa 60
gcactgaagt atgtttgaaa tgactcacct gtgataccta 100
<210>568
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>568
caggtaacat gggcctgctc cagcaacttg aggctgatat tggtaagtga taaagaaaga 60
tctctgtaaa tacttactga gttaatattt aaagttaaac 100
<210>569
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>569
cagggaatat ccttcaggat ctgcagccat ttcttgtcaa aacaaggtaa ggatttaatg 60
gttgatgaat tttggtaatg atttcctttt ttttttttta 100
<210>570
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>570
gattggtaga gctggtcgtg atggacttca aagttcttgt cacgtcctct gggctcctgc 60
agacattaac ttaaataggt aaaaaaaatt tattgttttt 100
<210>571
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>571
ttgggaatta tgggaactga aaaatgctgt gataattgca ggtccaggta aagatttctt 60
attatagatg gacattctaa aagtctttct ttctcttcct 100
<210>572
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>572
cgcccttacg aaaaaggtaa acggtgtagg agtctgcctg tttgacttaa ttttgtttcc 60
cactccacat taaaagatcc tttttgcttt taatagggta 100
<210>573
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>573
tgttccccca gctattctgg caacaaacaa gatactggtg gatatggcca aaatgaggta 60
aactatcttt tgcatgtgtt ctatttattt ccttctaaca 100
<210>574
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>574
agtctatggc catcacatac tctttattcc aagaaaagaa gatgcctttg gtaagtgtga 60
ctttcatgtt acagggaatt tttttagttt acttaaactt 100
<210>575
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>575
aagagatctg ttcaagttct aagagaagca aggaagaagt aggcatcaat actgaggtat 60
taattatata tagaattttc ataaagtgtc agtttgttca 100
<210>576
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>576
gatgcttgca ctggaatata aaacacacac acctattaga aacaggcagg gccagggctg 60
gccagtctaa ccaagatttc tcattaccat cttatttggc 100
<210>577
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>577
cagtaatgac aagaagccaa acatgtgtat gttttactgg aacttccaag gacctacaaa 60
gaaaggatag atgggatcag acctcagatg tgctggaaaa 100
<210>578
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>578
atcaactagg tccaaggcct gggtggctga tgagccaaag agaaacttca gcctgtgaaa 60
taaaaacact tcagattaga aggcctgatt ctcaaagtca 100
<210>579
<211>100
<212>DNA
<213> Artificial sequence (chemical Synthesis)
<400>579
aacactacgg ccatcgcgcc gcaggacacg ccctccacga ccggcggacc gccgcgacgc 60
tccagctgac tgcgcctacc tgtggaggat cctgaccccc 100

Claims (7)

1. A kit for detecting the effectiveness of a DNA homologous recombination repair pathway is characterized by comprising a probe library which is composed of probes shown in SEQ ID NO. 1-579 and aims at genes related to the DNA homologous recombination repair pathway.
2. The kit of claim 1, wherein the genes related to the homologous recombination repair pathway include LIG, MRE, NBN, PARG, PARP, PARPBP, RAD, TP53BP, XRCC, EXO, PCNA, POLD, RFC, RPA, BARD, BLM, BRCA, BRIP, DNA, EID, EME, ERCC, FANCM, FEN, GEN, H2AFX, HELQ, HFM, INO, KAT, MUS, NFF 2, NSMCE, NSMCATCE, NSMCLH 4, PALB, PARP, PAXILP, POQ, PPP4R, PPP4R, SLR 4, SLR 51, SLSC, SLR 51, SLR 3, SLR 51, SWR 51, SAD, EME, RFE, RECE, REC, RFN, RFL.
3. The kit for detecting the effectiveness of the DNA homologous recombination repair pathway of claim 1, wherein the probe sequence corresponding to gene LIG1 is shown in SEQ ID NO. 1-11, the probe sequence corresponding to gene MRE11 is shown in SEQ ID NO. 12-17, the probe sequence corresponding to gene NBN is shown in SEQ ID NO. 18-23, the probe sequence corresponding to gene PARG is shown in SEQ ID NO. 24-29, the probe sequence corresponding to gene PARP1 is shown in SEQ ID NO. 30-40, the probe sequence corresponding to gene PARPBP is shown in SEQ ID NO. 41-45, the probe sequence corresponding to gene RAD50 is shown in SEQ ID NO. 46-57, the probe sequence corresponding to gene TP53BP1 is shown in SEQ ID NO. 58-71, the probe sequence corresponding to gene XRCC2 is shown in SEQ ID NO. 72-74, and the probe sequence corresponding to XRCC3 is shown in SEQ ID NO.75, the probe sequence corresponding to the gene EXO1 is shown as SEQ ID No. 79-84, the probe sequence corresponding to the gene PCNA is shown as SEQ ID No. 85-88, the probe sequence corresponding to the gene POLD1 is shown as SEQ ID No. 89-101, the probe sequence corresponding to the gene POLD2 is shown as SEQ ID No. 102-106, the probe sequence corresponding to the gene POLD3 is shown as SEQ ID No. 107-110, the probe sequence corresponding to the gene POLD4 is shown as SEQ ID No. 111-113, the probe sequence corresponding to the gene RFC1 is shown as SEQ ID No. 114-123, the probe sequence corresponding to the gene RFC2 is shown as SEQ ID No. 124-127, the probe sequence corresponding to the gene RFC3 is shown as SEQ ID No.128 RFC 131, the probe sequence corresponding to the gene RFC4 is shown as SEQ ID No. 132-135, the probe sequence corresponding to the gene 5 is shown as SEQ ID No. 138, and the probe sequence corresponding to the gene RPA 829144, the probe sequence corresponding to the gene RPA2 is shown as SEQ ID NO. 145-148, the probe sequence corresponding to the gene RPA3 is shown as SEQ ID NO. 149-151, the probe sequence corresponding to the gene RPA4 is shown as SEQ ID NO. 152-153, the probe sequence corresponding to the gene BARD1 is shown as SEQ ID NO. 154-158, the probe sequence corresponding to the gene BLM is shown as SEQ ID NO. 159-169, the probe sequence corresponding to the gene BRCA1 is shown as SEQ ID NO. 170-178, the probe sequence corresponding to the gene BRCA2 is shown as SEQ ID NO. 179-194, the probe sequence corresponding to the gene BRIP1 is shown as SEQ ID NO. 195-204, the probe sequence corresponding to the gene DMC1 is shown as SEQ ID NO. 205-209, the probe sequence corresponding to the gene DNA 352 is shown as SEQ ID NO. 210-219, the probe sequence corresponding to the gene EID3 is shown as SEQ ID NO. 220-1, and the probe sequence corresponding to EMID NO. 222-2 25, the probe sequence corresponding to the gene EME2 is shown as SEQ ID NO. 226-229, the probe sequence corresponding to the gene ERCC1 is shown as SEQ ID NO. 230-232, the probe sequence corresponding to the gene FANCM is shown as SEQ ID NO. 233-245, the probe sequence corresponding to the gene FEN1 is shown as SEQ ID NO. 246-248, the probe sequence corresponding to the gene GEN1 is shown as SEQ ID NO. 249-254, the probe sequence corresponding to the gene H2AFX is shown as SEQ ID NO. 255-256, the probe sequence corresponding to the gene HELQ is shown as SEQ ID NO. 257-265, the probe sequence corresponding to the gene HFM1 is shown as SEQ ID NO. 266-280, the probe sequence corresponding to the gene INO80 is shown as SEQ ID NO. 281-294, the probe sequence corresponding to the gene KAT5 is shown as SEQ ID NO. 295-300, the probe sequence corresponding to the gene MUS81 is shown as SEQ ID NO. 307, and the probe sequence corresponding to NFID NO.32 is shown as SEQ ID NO. 308-2 IP, the probe sequence corresponding to the gene NSMCE1 is shown as SEQ ID NO. 311-315, the probe sequence corresponding to the gene NSMCE2 is shown as SEQ ID NO. 316-318, the probe sequence corresponding to the gene NSMCE3 is shown as SEQ ID NO. 319-320, the probe sequence corresponding to the gene NSMCE4A is shown as SEQ ID NO. 321-324, the probe sequence corresponding to the gene PALB2 is shown as SEQ ID NO. 325-331, the probe sequence corresponding to the gene PARP2 is shown as SEQ ID NO. 332-337, the probe sequence corresponding to the gene PAXIP1 is shown as SEQ ID NO. 338-346, the probe sequence corresponding to the gene POLH is shown as SEQ ID NO. 347-351, the probe sequence corresponding to the gene POLQ is shown as SEQ ID NO. 352-369, the probe sequence corresponding to the gene PPP4C is shown as SEQ ID NO. 370-372, the probe sequence corresponding to the gene PPP4R1 is shown as SEQ ID NO. 384-381, and the probe sequence corresponding to the gene 381-380 is shown as SEQ ID NO. 381-380, the probe sequence corresponding to the gene PPP4R4 is shown as SEQ ID NO. 385-394, the probe sequence corresponding to the gene RAD51 is shown as SEQ ID NO. 395-398, the probe sequence corresponding to the gene RAD51B is shown as SEQ ID NO. 399-402, the probe sequence corresponding to the gene RAD51C is shown as SEQ ID number 403-405, the probe sequence corresponding to the gene RAD51D is shown as SEQ ID number 406-409, the probe sequence corresponding to the gene RAD52 is shown as SEQ ID NO. 410-413, the probe sequence corresponding to the gene RAD54B is shown as SEQ ID number 414-419, the probe sequence corresponding to the gene RAD54L is shown as SEQ ID NO. 420-426, the probe sequence corresponding to the gene RBBP8 is shown as SEQ ID NO. 427-434, the probe sequence corresponding to the gene RDM1 is shown as SEQ ID number 435-439, and the probe sequence corresponding to the gene QL 445 is shown as SEQ ID NO.440, the probe sequence corresponding to the gene RECQL4 is shown as SEQ ID number 446-456, the probe sequence corresponding to the gene RECQL5 is shown as SEQ ID number 457-464, the probe sequence corresponding to the gene RMI1 is shown as SEQ ID number 465-468, the probe sequence corresponding to the gene RMI2 is shown as SEQ ID number 469-470, the probe sequence corresponding to the gene RTEL1 is shown as SEQ ID No. 471-483, the probe sequence corresponding to the gene SHFM1 is shown as SEQ ID number 484, the probe sequence corresponding to the gene SLX1A is shown as SEQ ID No. 485-489, the probe sequence corresponding to the gene SLX1B is shown as SEQ ID number 490-494, the probe sequence corresponding to the gene SLX4 is shown as SEQ ID No. 495-503, the probe sequence corresponding to the gene SMARCAD1 is shown as SEQ ID number 504-513, and the probe sequence corresponding to the gene SEQ ID No.5 is shown as SEQ ID No. 524-5, the probe sequence corresponding to the gene SMC6 is shown as SEQ ID number 525-536, the probe sequence corresponding to the gene SPO11 is shown as SEQ ID No. 537-540, the probe sequence corresponding to the gene SWSAP1 is shown as SEQ ID number 541-542, the probe sequence corresponding to the gene TOP3A is shown as SEQ ID number 543-550, the probe sequence corresponding to the gene TOP3B is shown as SEQ ID number 551-557, the probe sequence corresponding to the gene UIMC1 is shown as SEQ ID number 558-562, the probe sequence corresponding to the gene WRN is shown as SEQ ID number 563-575, and the probe sequence corresponding to the gene ZSQIM 7 is shown as SEQ ID number 576-579.
4. A method for detecting a gene associated with a DNA homologous recombination repair pathway, comprising the steps of:
(1) obtaining a DNA sample library of a subject;
(2) hybridizing all probes shown as SEQ ID NO. 1-579 in the kit of claim 1 with the DNA sample library;
(3) and (3) separating the hybridization product in the step (2), releasing the gene segment hybridized with the probe in the kit, and sequencing the separated gene segment by adopting a sequencing technology so as to detect the mutation condition of the gene.
5. The method according to claim 4, wherein the DNA sample library in the step (1) is composed of double-stranded DNA fragments;
the step (1) comprises the following steps: extracting whole genome DNA, and then fragmenting the whole genome DNA; or mRNA is extracted, fragmented, and then double-stranded cDNA is synthesized using the fragmented mRNA as a template.
6. The method according to claim 4, wherein the probe in the step (2) is selectively labeled.
7. The method according to claim 6, wherein the probe in the step (2) is labeled with biotin.
CN201911239803.1A 2019-12-06 2019-12-06 Probe library, detection method and kit for detecting effectiveness of DNA homologous recombination repair pathway Pending CN110863048A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201911239803.1A CN110863048A (en) 2019-12-06 2019-12-06 Probe library, detection method and kit for detecting effectiveness of DNA homologous recombination repair pathway

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201911239803.1A CN110863048A (en) 2019-12-06 2019-12-06 Probe library, detection method and kit for detecting effectiveness of DNA homologous recombination repair pathway

Publications (1)

Publication Number Publication Date
CN110863048A true CN110863048A (en) 2020-03-06

Family

ID=69657800

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201911239803.1A Pending CN110863048A (en) 2019-12-06 2019-12-06 Probe library, detection method and kit for detecting effectiveness of DNA homologous recombination repair pathway

Country Status (1)

Country Link
CN (1) CN110863048A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112280861A (en) * 2020-11-02 2021-01-29 魏尔啸实验室科技(杭州)有限公司 Detection system for detecting homologous recombination gene mutation HRRm
CN113862335A (en) * 2021-10-23 2021-12-31 南京世和基因生物技术股份有限公司 Probe library, detection method and kit for detecting back mutation
CN114014924A (en) * 2021-11-17 2022-02-08 安可来(重庆)生物医药科技有限公司 Method for improving homologous recombination efficiency in gene editing process through BRCA1 and BARD1 proteins
CN115982644A (en) * 2023-01-19 2023-04-18 中国医学科学院肿瘤医院 Esophageal squamous cell carcinoma classification model construction and data processing method

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103667254A (en) * 2012-09-18 2014-03-26 邵阳 Enrichment and detection method of target gene fragment
CN106834515A (en) * 2017-02-22 2017-06-13 南京世和基因生物技术有限公司 A kind of probe library of the exons mutation of detection MET genes 14, detection method and kit
CN108148891A (en) * 2018-03-05 2018-06-12 重庆市肿瘤研究所 The probe preparation method of DNA of tumor cell injury repair related gene capture sequencing

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103667254A (en) * 2012-09-18 2014-03-26 邵阳 Enrichment and detection method of target gene fragment
CN106834515A (en) * 2017-02-22 2017-06-13 南京世和基因生物技术有限公司 A kind of probe library of the exons mutation of detection MET genes 14, detection method and kit
CN108148891A (en) * 2018-03-05 2018-06-12 重庆市肿瘤研究所 The probe preparation method of DNA of tumor cell injury repair related gene capture sequencing

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MARK R. KELLEY等主编: "《DNA Repair in Cancer Therapy Molecular Targets and Clinical Applications》", 7 June 2016, MICA HALEY, pages: 8 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112280861A (en) * 2020-11-02 2021-01-29 魏尔啸实验室科技(杭州)有限公司 Detection system for detecting homologous recombination gene mutation HRRm
CN113862335A (en) * 2021-10-23 2021-12-31 南京世和基因生物技术股份有限公司 Probe library, detection method and kit for detecting back mutation
CN114014924A (en) * 2021-11-17 2022-02-08 安可来(重庆)生物医药科技有限公司 Method for improving homologous recombination efficiency in gene editing process through BRCA1 and BARD1 proteins
CN115982644A (en) * 2023-01-19 2023-04-18 中国医学科学院肿瘤医院 Esophageal squamous cell carcinoma classification model construction and data processing method
CN115982644B (en) * 2023-01-19 2024-04-30 中国医学科学院肿瘤医院 Esophageal squamous cell carcinoma classification model construction and data processing method

Similar Documents

Publication Publication Date Title
CN110863048A (en) Probe library, detection method and kit for detecting effectiveness of DNA homologous recombination repair pathway
AU2019250224B2 (en) Enhanced transgene expression and processing
AU2020223686B2 (en) Plant regulatory elements and uses thereof
KR102114412B1 (en) PREDICTING GASTROENTEROPANCREATIC NEUROENDOCRINE NEOPLASMS (GEP-NENs)
KR102555447B1 (en) Methods and products for quantifying rna transcript variants
KR20170099939A (en) Sequencing control
WO1998045435A2 (en) SECRETED EXPRESSED SEQUENCE TAGS (sESTs)
EP0593580A4 (en) Sequences characteristic of human gene transcription product
KR20210107057A (en) Targeted Integration of Nucleic Acids
JPH05236965A (en) Trkc protein
CA2497597A1 (en) Methods for identifying subjects at risk of melanoma and treatments
CN111410695B (en) Chimeric molecule based on autophagy mechanism mediated Tau protein degradation and application thereof
JP2009531032A (en) Use of protein S fusion to solubilize proteins
KR20200004977A (en) Probes for detecting gene mutations for cancer, method for producing the same and method for detecting cancer using thereof
JP2001501809A (en) Novel TFIIB transcription factor from Candida albicans, nucleic acid sequence encoding the same, and screening method for inhibiting Candida albicans growth
CN110863043A (en) Probe library, detection method and kit for detecting validity of DNA repair pathway of Vanconi anemia
CN113151447A (en) Capture probe and kit for primary atopic disease related gene and application thereof
KR20210095859A (en) Nucleic Acids for Cell Recognition and Integration
CN110863049A (en) Probe library, detection method and kit for detecting effectiveness of DNA mismatch repair pathway
WO2020081588A1 (en) Swi/snf family chromatin remodeling complexes and uses thereof
CN114807209B (en) Method for improving conversion efficiency of saccharomyces cerevisiae DNA fragments
KR20070055860A (en) Methods of screening anticancer agent by using dnp63 promoter
CN109295163B (en) Universal long-fragment chromosome walking method
CN112359096A (en) Kit for detecting key mutant gene of DNA base excision repair pathway
CN110846416A (en) Probe library, detection method and kit for detecting effectiveness of DNA non-homologous end connection repair pathway

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20200306

RJ01 Rejection of invention patent application after publication