CN114807209B

CN114807209B - Method for improving conversion efficiency of saccharomyces cerevisiae DNA fragments

Info

Publication number: CN114807209B
Application number: CN202210322772.1A
Authority: CN
Inventors: 杜军; 谢天; 卢修亮; 李涛; 岳方正; 王文朋; 王早霞
Original assignee: Biological Disaster Prevention And Control Center Of State Forestry And Grassland Administration; Tsingke Biotechnology Co Ltd
Current assignee: Biological Disaster Prevention And Control Center Of State Forestry And Grassland Administration; Tsingke Biotechnology Co Ltd
Priority date: 2022-03-30
Filing date: 2022-03-30
Publication date: 2023-11-17
Anticipated expiration: 2042-03-30
Also published as: CN114807209A

Abstract

The invention belongs to the field of synthetic biology, and particularly relates to a method for improving the conversion efficiency of DNA fragments in a host so as to achieve the purpose of industrialized batch assembly of large DNA fragments. The method provided by the invention has the advantages that after the target fragment is subjected to methylation modification, the conversion efficiency is improved, the same amount of DNA is used, the number of transformants growing on a flat plate after conversion coating is obviously improved, the possibility of screening positive clones is greatly improved, the amount of DNA to be assembled is less, the material cost, the labor cost and the time cost of production are reduced, and the method is favorable for industrial production of large-fragment DNA synthesis.

Description

Method for improving conversion efficiency of saccharomyces cerevisiae DNA fragments

Technical field:

the invention belongs to the field of synthetic biology, and particularly relates to a method for improving the conversion efficiency of DNA fragments in a host so as to achieve the purpose of industrialized batch assembly of large DNA fragments.

The background technology is as follows:

synthetic biology is an emerging discipline developed in recent years, and the main objective is to design and construct diverse new devices, networks and biological pathways through DNA-encoded biological component modules, wherein the most critical is how to synthesize or modify genes, and thus, DNA synthesis or modification becomes a key point of synthetic biology. Microorganisms are capable of synthesizing various metabolites, and the synthetase genes of many metabolites tend to exist in clusters and tend to be large, usually several tens or even hundreds of kb, so that it is difficult to synthesize them by chemical synthesis or PCR. Currently, researchers have developed methods for preparing large DNA fragments based on Saccharomyces cerevisiae or E.coli.

Saccharomyces cerevisiae not only has wide application in industry and food, but also has wide application in molecular biology research due to the advantages of clear genetic background, high homologous recombination efficiency and the like. As a first fully sequenced eukaryotic microorganism, saccharomyces cerevisiae is of great importance in molecular biology and biosynthesis of valuable products. The large fragment assembly technology in the synthetic biology is mostly based on the homologous recombination capability of saccharomyces cerevisiae cells, and particularly, a target large fragment is designed into a plurality of small fragments of 5-8kb in advance, the 5-8kb fragments are constructed by using traditional methods of enzyme digestion, enzyme ligation, in vitro homologous recombination and the like, the homologous arms of 70-100bp are contained between each fragment, all fragments and linearization vectors are transformed into the saccharomyces cerevisiae cells, the cells can recognize the homologous arms between the fragments, and recombination is carried out by utilizing a DNA fragmentation repair system in the living cells of the saccharomyces to form an annular complete plasmid.

For example, chinese patent No. CN201310389392.0 discloses a method for rapidly assembling yeast with multi-fragment DNA, which comprises the following steps: (1) Co-transforming a plurality of DNA molecules comprising homology arms with a linearized yeast shuttle vector to yeast; (2) Eluting all transformed yeast colonies on the whole screening culture plate, centrifuging, and discarding the supernatant to obtain transformed yeast cells; (3) Extracting plasmid DNA of the saccharomycete cells obtained in the step (2), and converting competent cells of the escherichia coli; (4) screening the escherichia coli clone to obtain large fragment DNA. The method of the invention has the advantages of high speed, simplicity, feasibility, high success rate, low cost, high efficiency and easy operation, can assemble a plurality of small-segment DNA molecules into one large-segment DNA molecule, is favorable for industrial scale expansion and has wide application.

It will be appreciated that only all small fragments are transformed into the same cell, and that the yeast can assemble the fragments in sequence. It is well known that Saccharomyces cerevisiae has a very low conversion efficiency, typically only up to 10 ³ -10 ⁴ CFU/. Mu.gDNA. In our experiments, it was found that one of the important reasons for the low transformation efficiency of Saccharomyces cerevisiae is that the fragment is excised by the defense system-nuclease system of the yeast cells after transformation into the yeast cells. Therefore, it is particularly important to solve this problem so as to greatly improve the conversion efficiency of large fragments in the Saccharomyces cerevisiae system.

The invention comprises the following steps:

in order to solve the technical problems, the invention provides a method for improving the conversion efficiency of a Saccharomyces cerevisiae DNA fragment, in particular to improving the conversion efficiency of a DNA large fragment in a Saccharomyces cerevisiae system.

The method has simple operation and high success rate, is suitable for large-scale production, and can convert the fragment with 0.1-1Mb from 10 in Saccharomyces cerevisiae ³ -10 ⁴ CFU/. Mu.g DNA was increased to 10 ⁴ -10 ⁵ CFU/. Mu.gDNA, the transformation efficiency is improved by about 10 times.

The invention provides a method for improving the conversion efficiency of saccharomyces cerevisiae DNA fragments, which comprises the following steps:

(1) Synthesizing target DNA fragment: dividing the DNA fragment of 0.1-100kb or the macromolecular fragment to be synthesized of 20kb-1Mb into a plurality of fragments of 0.1-100kb for synthesis;

the DNA fragment may be linearized DNA or circularized plasmid DNA;

(2) Carrying out methylation modification on the DNA fragment or the split fragment obtained in the step (1);

further, the methylation modification is obtained by methyltransferase treatment;

still further, the methyltransferase may be at least one of the following methyltransferases: enzymes having methylation-modified DNA such as CpG methyltransferase, gpC methyltransferase, dam methyltransferase, dcm methyltransferase, ecoRI methyltransferase, hpaII methyltransferase, mspI methyltransferase, hhaI methyltransferase, taq I methyltransferase, aluI methyltransferase, bamHI methyltransferase, haeIII methyltransferase, dpnM methyltransferase, human DNA methyltransferase, ecoGII methyltransferase and the like;

preferably, the methyltransferase is a methyltransferase having a recognition sequence of only 2 or 4 bases, such as CpG methyltransferase, gpC methyltransferase, taq I methyltransferase, dpnM methyltransferase, etc.;

more preferably, the methyltransferase is selected from the group consisting of 2 enzyme combinations or 3 enzyme combinations or 4 enzyme combinations of CpG methyltransferase, gpC methyltransferase, taq I methyltransferase, dpnM methyltransferase;

more preferably, the methyltransferase is combined with CpG methyltransferase, gpC methyltransferase, taq I methyltransferase, dpnM methyltransferase in an enzyme activity ratio of 4:3:1:2;

further, the methylation modification system is as follows (50 μl reaction): adding 2-5. Mu.g of DNA fragment and 1-10U of methyltransferase, adding S-adenosylmethionine with a final concentration of 80-160. Mu.M, 5. Mu.L of 10 Xreaction buffer, ddH ₂ O is added to 50 mu L, and the mixture is reacted in a water bath at 37-65 ℃ for 1-5h;

furthermore, the methylation modification system is a 50 mu L reaction system, and can be amplified or reduced in a same ratio in practical application; further, the reaction buffer consists of: 200mM Tris-Ac (pH 7.9), 500mM KAC,100mM MgAc2,100mM DTT,1000. Mu.g/ml Albumin (Albumin), the remainder being water;

(3) The DNA fragments after methylation modification or the DNA fragments after resolution after methylation modification and the linearized plasmid are transformed into yeast cells together for assembly or transformation;

further, the DNA fragment is designed with a homologous arm connected with a plasmid, and homologous arms capable of being sequentially connected into full-length fragments are arranged between the split fragments;

further, the length of the homology arm is 25-150bp, the length of the preferable homology arm is 50-100bp, and the length of the more preferable homology arm is 100bp;

further, the plasmid can be any one of yeast vector or yeast and escherichia coli shuttle vector, and can be a public general plasmid or an engineered plasmid: such as pGADT7, pGBKT7, pRS415, pPICZC, pYX212 and the like;

the transformation method can be various, such as PEG-LiAc method, electrotransformation method, etc., and it is understood that the method of the invention modifies the DNA to be transformed so that the DNA is not excised by nuclease system in living cells, thereby improving the transformation efficiency of DNA fragments, and the invention can be applied to any transformation method.

The beneficial effects are that:

1. according to the technical scheme provided by the invention, the quantity of the transformants growing on the plate after the transformation plating is obviously increased due to the improvement of the transformation efficiency and the same DNA consumption, so that the possibility of screening positive clones is greatly improved (under the condition that too few transformants exist, all transformants are always screened and positive clones cannot be found);

2. according to the technical scheme provided by the invention, as methylation modification protects the transformed nucleic acid from degradation, in a single cell, the nucleic acid molecule exists for a longer time, which is more beneficial for the living cells to assemble the fragments, namely the positive rate after transformation is greatly improved;

3. according to the technical scheme provided by the invention, the conversion efficiency is improved, so that the amount of DNA to be assembled is less, the material cost, the labor cost and the time cost of production are reduced, and the industrial production of large-segment DNA synthesis is facilitated.

Description of the drawings:

FIG. 1 unmodified sequence;

FIG. 2 shows the sequence modified by DpnM methyltransferase.

The specific embodiment is as follows:

the invention is described below by means of specific embodiments. The technical means used in the present invention are methods well known to those skilled in the art unless specifically stated. Further, the embodiments should be construed as illustrative, and not limiting the scope of the invention, which is defined solely by the claims. Various changes or modifications to the materials ingredients and amounts used in these embodiments will be apparent to those skilled in the art without departing from the spirit and scope of the invention.

When a DNA sequence is transformed into a yeast cell, the yeast cell nuclease system recognizes several bases and cleaves it. After modification of DNA, the site is protected and not recognized, thus playing a role in protection.

The sites recognized by one nuclease in the yeast nuclease system are assumed to be: CTGATCTC (grey mark in fig. 1) and treatment of this DNA with DpnM methyltransferase (recognizes GATC sequence) (fig. 2) will modify the GATC sequence into grey parts of fig. 2, and the modified sequence cannot be recognized by the yeast nuclease system, i.e. cannot be excised, i.e. protection is achieved.

It is noted that yeast nuclease systems contain a variety of nucleases, the recognition sites of which have not been reported so far, and therefore the relationship between the manner of methyltransferase modification and the protection against yeast nuclease systems is not clear.

The invention provides a method for improving the conversion efficiency of a DNA fragment in a host cell, which comprises the steps of introducing the DNA fragment to be converted into the host cell for conversion after methylation modification, so that the conversion efficiency is improved, the number of transformants growing on a plate after conversion coating is obviously improved, and the possibility of screening positive clones is greatly improved;

the DNA fragment may be linearized DNA or circularized plasmid DNA; the length of the DNA fragment can be from 100bp-5000bp or 5000bp-20000bp or 20000bp-100000bp or more than 100000bp;

the method is suitable for the DNA fragments of 0.1-100kb, and is also suitable for the DNA large fragments of more than 20kb or 100kb which need to be further assembled; for large DNA fragments larger than 20kb or 100kb, the large DNA fragments can be split into a plurality of DNA fragments of 0.1-100kb, and after methylation modification, transformation and assembly are completed in host cells;

in the present invention, 50. Mu.L of methylation modification system was used as follows: adding 2-5 muThe gDNA fragment was mixed with 1-10U methyltransferase, S-adenosylmethionine was added at a final concentration of 80-160. Mu.M, 5. Mu.L of 10 Xreaction buffer, ddH ₂ O is added to 50 mu L, and the mixture is reacted in a water bath at 37-65 ℃ for 1-5h;

furthermore, the methylation modification system is a 50 mu L reaction system, and can be amplified or reduced in a same ratio in practical application;

in the invention, the types of DNA fragments in a methylation system can be one or more, if the large DNA fragment to be assembled is 160kb, the large DNA fragment can be split into about 32 fragments of 5000bp, and the 32 fragments are simultaneously added into the methylation system, and then introduced into a host cell for transformation and assembly after the methylation reaction is completed;

in the present invention, the 10 x reaction buffer composition used for methylation modification is: 200mM Tris-Ac (pH 7.9), 500mM KAC,100mM MgAc2,100mM DTT,1000. Mu.g/ml Albumin (Albumin);

in the present invention, methyltransferases used for methylation modification include, but are not limited to: enzymes having methylation-modified DNA such as CpG methyltransferase, gpC methyltransferase, dam methyltransferase, dcm methyltransferase, ecoRI methyltransferase, hpaII methyltransferase, mspI methyltransferase, hhaI methyltransferase, taq I methyltransferase, aluI methyltransferase, bamHI methyltransferase, haeIII methyltransferase, dpnM methyltransferase, human DNA methyltransferase, ecoGII methyltransferase and the like;

in the present invention, the methyltransferase used for methylation modification may be a combination of one, two, three or four or more of the above methyltransferases; preferably, it is a combination of two or more of CpG methyltransferase, gpC methyltransferase, taq I methyltransferase and DpnM methyltransferase; more preferably, the CpG methyltransferase, the GpC methyltransferase, the Taq I methyltransferase and the DpnM methyltransferase are used in combination in a ratio of 4:3:1:2;

in the invention, for the target DNA fragment of 0.1-100kb, methylation modification can be directly carried out, and then the target DNA fragment and linearized plasmid are transformed into a yeast host cell together; the DNA fragment is designed with a homology arm for connecting plasmids;

in the invention, for the target DNA fragment of more than 20kb or 100kb, after methylation modification, a plurality of split DNA fragments of 0.1-100kb are transformed into yeast host cells together with linearized plasmids for transformation and assembly to obtain the target DNA fragment of more than 20kb or 100 kb; the DNA fragments are provided with homologous arms which can be sequentially connected to obtain full-length fragments, and the first and the last fragments are designed with homologous arms connected with plasmids;

the length of the adopted homology arm is 25-150bp, the length of the preferable homology arm is 50-100bp, and the length of the more preferable homology arm is 100bp;

in the invention, the adopted plasmid can be any one of yeast vector or yeast and escherichia coli shuttle vector, and can be a public general plasmid or an altered plasmid, such as pGADT7, pGBKT7, pRS415, pPICZC, pYX212 and the like;

the transformation method adopted by the invention can be various, such as PEG-LiAc method, electric transformation method and the like, and it is understood that the method of the invention modifies the DNA to be transformed so that the DNA is not excised by a nuclease system in living cells, and the transformation efficiency of the DNA fragments is improved, thus the invention can be applied to any transformation method.

The invention will be further explained by means of specific embodiments.

Example 1A method for improving the conversion efficiency of DNA fragment Yeast

The embodiment fits into a DNA fragment (shown as SEQ ID NO. 1) of about 6000bp, homologous arms 1 and 2 connected with plasmids are arranged at two ends of the fragment, and then the fragment is obtained by a chemical synthesis method;

homology arm 1:atgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaa aacgacggccag

homology arm 2:ttcctgtgtgaaattgttatccgctcgccggcggctgctaacaaagcccgaaaggaagct gagttggctgctgcca

after methylation modification of the synthetic fragment, the synthetic fragment was introduced into yeast cells together with linearized plasmid pRS415 for transformation as follows:

(1) 42 μg of the above synthetic fragment was distributed on average into 21 tubes 1.5ml centrifuge tubes, each tube being numbered: 1-21;

(2) To each of the above tubes, 10 Xreaction buffer (200 mM Tris-Ac (pH 7.9), 500mM KAC,100mM MgAc2,100mM DTT,1000. Mu.g/ml Albumin (Albumin)) 5. Mu.L, ademetionine 160. Mu.M, and ddH were added ₂ O is added to 47 mu L;

(3) Add 3. Mu.L ddH to tube 1 ₂ O；

Add 3. Mu.L of DpnM methyltransferase to tube 2;

add 3. Mu. LCpG I methyltransferase to tube 3;

add 3. Mu.L of GpC I methyltransferase to tube 4;

add 3. Mu.L of dam methyltransferase to tube 5;

3 μ LTaq I methyltransferase was added to tube 6;

1.5. Mu.L of DpnM methyltransferase, 1.5. Mu.L of CpG I methyltransferase were added to tube 7;

1.5. Mu.L CpG I methyltransferase, 1.5. Mu.L GpC I methyltransferase were added to tube 8;

1.5. Mu.L of DpnM methyltransferase, 1.5. Mu.L of dam methyltransferase were added to tube 9;

to tube 10, 1.5. Mu.L of DpnM methyltransferase, 1.5. Mu.L of Taq I methyltransferase were added;

to tube 11, 1. Mu.LCpG I methyltransferase, 1. Mu.L GpC I methyltransferase, 1. Mu.L DpnM methyltransferase were added;

to tube 12, 1. Mu.LCpG I methyltransferase, 1. Mu.L GpC I methyltransferase, 1. Mu.L Taq I methyltransferase were added;

to tube 13, 1. Mu.L of GpC I methyltransferase, 1. Mu.L of Taq I methyltransferase were added;

to tube 14, 1. Mu.L of DpnM methyltransferase, 1. Mu.L of Taq I methyltransferase were added;

to tube 15, cpGI methyltransferase 0.75. Mu.L, gpC I methyltransferase 0.75. Mu.L, taq I methyltransferase 0.75. Mu.L and DpnM methyltransferase 0.75. Mu.L were added;

to tube 16, cpGI methyltransferase 0.3. Mu.L, gpC I methyltransferase 0.6. Mu.L, taq I methyltransferase 0.9. Mu.L and DpnM methyltransferase 1.2. Mu.L were added;

to tube 17, cpGI methyltransferase 0.6. Mu.L, gpC I methyltransferase 0.3. Mu.L, taq I methyltransferase 1.2. Mu.L and DpnM methyltransferase 0.9. Mu.L were added;

to tube 18 was added CpGI methyltransferase 1.2. Mu.L, gpC I methyltransferase 0.9. Mu.L, taq I methyltransferase 0.3. Mu.L and DpnM methyltransferase 0.6. Mu.L;

to tube 19 was added CpGI methyltransferase 0.6. Mu.L, gpC I methyltransferase 0.6. Mu.L, taq I methyltransferase 0.6. Mu. L, dpnM methyltransferase 0.6. Mu. L, dam methyltransferase 0.6. Mu.L;

to tube 20 was added CpGI methyltransferase 0.5. Mu.L, gpC I methyltransferase 0.5. Mu.L, taq I methyltransferase 0.5. Mu. L, dpnM methyltransferase 0.5. Mu. L, dam methyltransferase 0.5. Mu. L, dcm methyltransferase 0.5. Mu.L;

to tube 21 was added CpGI methyltransferase 0.5. Mu.L, gpC I methyltransferase 0.5. Mu.L, taq I methyltransferase 0.5. Mu. L, dpnM methyltransferase 0.5. Mu. L, dam methyltransferase 0.5. Mu. L, bamHI methyltransferase 0.5. Mu.L;

the enzyme activity unit of each mu L of internal methyltransferase is 1U;

(4) Shaking and mixing uniformly, and placing in a 37 ℃ water bath for 1h; cleaning and recycling the modified DNA by using a PCR cleaning and recycling kit;

(5) 10ng of each recovered product is converted into Saccharomyces cerevisiae BY4741 BY using a PEG-LiAc method; the specific transformation method comprises the following steps: adding 50%PEG3350,1.0M LiAc,10mg/mL ssDNA derived from salmon monomer and 10ng (4) recovered product and 5ng linearized plasmid pRS415 into competent cells of Saccharomyces cerevisiae, mixing thoroughly, incubating at 30deg.C for 30min, adding DMSO with final concentration of 10%, blowing and mixing, heat-shock at 42deg.C for 25min, centrifuging to collect yeast cells, adding 5mM CaCl ₂ Incubating at 25deg.C for 10min, adding YPDA culture medium, culturing at 30deg.C for 90min, diluting with 10 times gradient for 5 gradients, applying SD-leu defect culture medium, and culturing in a 30 deg.C incubator; after 48 hours of cultivation, transformant statistics were carried out, and the results are shown in Table 1 below.

Total number of transformants = colony number x dilution x total volume of stock solution of conversion reaction/volume of plating bacteria solution;

conversion efficiency = total number of transformants/amount of DNA added (μg).

TABLE 1

Tube count	Total number of transformants	Conversion efficiency (CFU/. Mu.g)
			Tube 1	(4.3±0.42)×10 ²	(4.3±0.42)×10 ⁴
Tube 2	(3.2±0.26)×10 ²	(3.2±0.26)×10 ⁴
			Tube 3	(6.4±0.52)×10 ²	(6.4±0.52)×10 ⁴
Tube 4	(7.4±0.50)×10 ²	(7.4±0.50)×10 ⁴
			Tube 5	(4.2±0.34)×10 ²	(4.2±0.34)×10 ⁴
Tube 6	(3.1±0.47)×10 ²	(3.1±0.37)×10 ⁴
			Tube 7	(7.0±0.62)×10 ²	(7.0±0.62)×10 ⁴
Tube 8	(7.2±0.52)×10 ²	(7.2±0.52)×10 ⁴
			Tube 9	(1.9±0.14)×10 ²	(1.9±0.14)×10 ⁴
Tube 10	(4.2±0.54)×10 ²	(4.2±0.54)×10 ⁴
			Tube 11	(7.2±0.87)×10 ²	(7.2±0.87)×10 ⁴
Tube 12	(3.7±0.62)×10 ²	(3.7±0.62)×10 ⁴
			Tube 13	(4.9±0.38)×10 ²	(4.9±0.38)×10 ⁴
Tube 14	(4.3±0.45)×10 ²	(4.3±0.45)×10 ⁴
			Tube 15	(9.0±0.86)×10 ²	(9.0±0.86)×10 ⁴
Tube 16	(6.2±0.88)×10 ²	(6.2±0.88)×10 ⁴
			Tube 17	(4.3±0.24)×10 ²	(4.3±0.24)×10 ⁴
Tube 18	(3.4±0.46)×10 ³	(3.4±0.46)×10 ⁵
			Tube 19	(1.0±0.12)×10 ³	(1.0±0.12)×10 ⁵
Tube 20	(7.6±0.64)×10 ²	(7.6±0.64)×10 ⁴
			Tube 21	(8.1±0.42)×10 ²	(8.1±0.42)×10 ⁴

From the above experimental results, it was found that the transformation efficiency was improved to some extent as compared with the unmethylated control tube 1, but the transformation efficiency was changed irregularly, which was consistent with the current situation that the yeast nuclease system contained a plurality of nucleases, but the recognition sites were not clear. Among them, it was unexpectedly found that when CpGI methyltransferase, gpCI methyltransferase, taq I methyltransferase and DpnM methyltransferase were combined in a ratio of 4:3:1:2, the conversion efficiency was significantly higher than other experimental groups, and the conversion rate was improved by nearly ten times as compared to the blank group.

Example 2A method for improving the conversion efficiency of DNA Large fragment Yeast

The present example fits into a large 120Kb DNA fragment, the fragment is split into 11 fragments of 5-20Kb (respectively fragment 1, fragment 2, fragment 3, fragment 4, fragment 5, fragment 6 and fragment … … fragment 11, and homology arms are arranged on the fragments in a pairwise sequence, the sequences are shown in SEQ ID NO.1-11, homology arm 1 is connected to the 5 'end of fragment 1, homology arm 2 is connected to the 3' end of sequence 11, the homology arm is the same as in example 1), the 11 fragments are obtained by a chemical synthesis method, and after methylation modification is carried out on the 11 fragments, the 11 fragments and linearized plasmid pRS415 are introduced into yeast cells together for transformation and assembly, and the specific procedures are as follows: (1) 84 μg of the 11 fragment mixture was distributed on average into 21 tubes 1.5ml centrifuge tubes, each tube being numbered: 1-21; (2) To each of the above-mentioned tubes, 10 Xreaction buffer (same as in example 1) 5. Mu.L, ademetionine 160. Mu.M, and ddH were added ₂ O is added to 47 mu L;

(3) Add 3. Mu.L ddH2O to tube 1;

add 3. Mu.L of DpnM methyltransferase to tube 2;

add 3. Mu.L CpG I methyltransferase to tube 3;

add 3. Mu.L of GpC I methyltransferase to tube 4;

add 3. Mu.L of dam methyltransferase to tube 5;

3 μ LTaq I methyltransferase was added to tube 6;

add 0.75. Mu.L of CpG I methyltransferase, 0.75. Mu.L of GpC I methyltransferase, 0.75. Mu.L of Taq I methyltransferase and 0.75. Mu.L of DpnM methyltransferase to tube 15;

to tube 16, 0.3. Mu.L of CpG I methyltransferase, 0.6. Mu.L of GpC I methyltransferase, 0.9. Mu.L of Taq I methyltransferase and 1.2. Mu.L of DpnM methyltransferase were added;

to tube 17, 0.6. Mu.L of CpG I methyltransferase, 0.3. Mu.L of GpC I methyltransferase, 1.2. Mu.L of Taq I methyltransferase and 0.9. Mu.L of DpnM methyltransferase were added;

to tube 18, 1.2. Mu.L of CpG I methyltransferase, 0.9. Mu.L of GpC I methyltransferase, 0.3. Mu.L of Taq I methyltransferase and 0.6. Mu.L of DpnM methyltransferase were added;

to tube 19, 0.6. Mu.L of CpG I methyltransferase, 0.6. Mu.L of GpC I methyltransferase, 0.6. Mu. L, dpnM methyltransferase, 0.6. Mu. L, dam methyltransferase and 0.6. Mu.L of Taq I methyltransferase were added;

to the tube 20, 0.5. Mu.L of CpG I methyltransferase, 0.5. Mu.L of GpC I methyltransferase, 0.5. Mu. L, dpnM methyltransferase, 0.5. Mu. L, dam methyltransferase, 0.5. Mu. L, dcm methyltransferase, 0.5. Mu.L of Taq I methyltransferase were added;

to tube 21, 0.5. Mu.L of CpG I methyltransferase, 0.5. Mu.L of GpC I methyltransferase, 0.5. Mu. L, dpnM methyltransferase, 0.5. Mu. L, dam methyltransferase, 0.5. Mu. L, bamHI methyltransferase and 0.5. Mu.L of Taq I methyltransferase were added;

the enzyme activity unit of each mu L of internal methyltransferase is 3U;

(4) Shaking and mixing uniformly, and placing in a 37 ℃ water bath for 1h; cleaning and recovering the modified DNA by using a PCR cleaning and recovering kit;

(5) 10ng of each recovered product was transformed into Saccharomyces cerevisiae BY4741 BY the PEG-LiAc method; the transformation method is the same as in example 1, SD-leu defect culture medium is coated, and the substrate is placed in a 30 ℃ incubator for culture; after 48 hours of cultivation, transformant statistics were carried out, and the results are shown in Table 2 below.

TABLE 2

Tube count	Total number of transformants	Conversion efficiency (CFU/. Mu.g)
			Tube 1	(5.0±0.48)×10	(5.0±0.48)×10 ²
Tube 2	(3.3±0.42)×10	(3.3±0.42)×10 ²
			Tube 3	(7.2±0.59)×10	(7.2±0.59)×10 ²
Tube 4	(1.0±0.13)×10 ²	(1.0±0.13)×10 ⁴
			Tube 5	(1.2±0.15)×10 ²	(1.2±0.15)×10 ⁴
Tube 6	(2.4±0.33)×10 ²	(2.4±0.33)×10 ⁴
			Tube 7	(6.4±0.56)×10 ²	(6.4±0.56)×10 ⁴
Tube 8	(6.8±0.67)×10 ²	(6.8±0.67)×10 ⁴
			Tube 9	(2.2±0.14)×10 ²	(2.2±0.14)×10 ⁴
Tube 10	(3.4±0.24)×10 ²	(3.4±0.24)×10 ⁴
			Tube 11	(7.4±0.48)×10 ²	(7.4±0.48)×10 ⁴
Tube 12	(6.2±0.52)×10 ²	(6.2±0.52)×10 ⁴
			Tube 13	(5.0±0.44)×10 ²	(5.0±0.44)×10 ⁴
Tube 14	(3.8±0.37)×10 ²	(3.8±0.37)×10 ⁴
			Tube 15	(8.2±0.34)×10 ²	(8.2±0.34)×10 ⁴
Tube 16	(3.0±0.18)×10 ²	(3.0±0.18)×10 ⁴
			Tube 17	(4.1±0.26)×10 ²	(4.1±0.26)×10 ⁴
Tube 18	(1.1±0.16)×10 ³	(1.1±0.16)×10 ⁵
			Tube 19	(9.3±0.46)×10 ²	(9.3±0.46)×10 ⁴
Tube 20	(7.0±0.38)×10 ²	(7.0±0.38)×10 ⁴
			Tube 21	(3.6±0.24)×10 ²	(3.8±0.24)×10 ⁴

From the above experimental results, it was found that the conversion efficiency was improved to some extent compared with the unmethylated blank tube 1 after the methylation treatment of the DNA fragment with different methyltransferases. Among them, it was unexpectedly found that when CpGI methyltransferase, gpC I methyltransferase, taq I methyltransferase and DpnM methyltransferase were combined in a ratio of 4:3:1:2, the conversion efficiency was significantly higher than other experimental groups, and the conversion rate was improved by a factor of ten times as compared to the blank group.

Example 3A method for improving the conversion efficiency of DNA Large fragment Yeast

Methylation of A, B, C, D different large fragments (4 fragments with sequences shown in SEQ ID NO.8-11, 5 'ends of sequences 8, 9, 10, 11 connected to homology arm 1, 3' ends of sequences 8, 9, 10, 11 connected to homology arm 2, and then synthesized) was performed using the optimal combination (18 tubes) in example 2:

homology arm 2:ttcctgtgtgaaattgttatccgctcgccggcggctgctaacaaagcccgaaaggaagct gagttggctgctgcca；

the above fragments were subjected to methylation modification after chemical synthesis, the modification method and system were the same as in tube 18 of example 2, while unmethylated fragments were set as controls, the remaining Saccharomyces cerevisiae transformation conditions were the same as in example 2, and the number of transformants was as shown in Table 3 below:

TABLE 3 Table 3

A	A-control	B	B-control
				Number of transformants	(2.2±0.32)×10 ³	(3.7±0.30)×10 ²	(4.0±0.27)×10 ³	(6.2±0.62)×10 ²
C	C-control	D	D-control
				Number of transformants	(4.4±0.28)×10 ³	(8.1±0.53)×10 ²	(2.4±0.45)×10 ³	(5.7±0.37)×10 ²

From the above experimental results, it was found that when CpG I methyltransferase, gpC I methyltransferase, taq I methyltransferase and DpnM methyltransferase were combined in a ratio of 4:3:1:2, the conversion efficiency was improved 3-7 times as compared with the unmethylated blank.

The above examples merely represent a few embodiments of the present invention, which are described in more detail and are not to be construed as limiting the scope of the patent. It should be noted that, for a person skilled in the art, the above embodiments may also make several variations, combinations and improvements, without departing from the scope of the present patent. Therefore, the protection scope of the patent is subject to the claims.

SEQUENCE LISTING

<110> Beijing Engine biotechnology Co., ltd

Biological disaster prevention and control center for national forestry and grassland bureau

<120> a method for improving the conversion efficiency of Saccharomyces cerevisiae DNA fragment

<130> 1

<160> 11

<170> PatentIn version 3.5

<210> 1

<211> 6433

<212> DNA

<213> artificial sequence

<400> 1

tagctgggtt ttccttgttg ctattttaaa aggtgattca tggagaactg gagatatgga 60

gtgtgaatgg acatgagtga gataagcagt ggatgtgtgt ggcagtttct gaccagggtg 120

tctctgtgtt tgcaggtgtc cagtgtgagg tgcagctggt ggagtccggg ggaggcttag 180

ttcagcctgg ggggtccctg agactctcct gtgcagcctc tggattcacc ttcagtagct 240

actggatgca ctgggtccgc caagctccag ggaaggggct ggtgtgggtc tcacgtatta 300

atagtgatga ctccgtgaag ggccgattca ccatctccag agacaacgcc aagaacacgc 360

tgtatctgca aatgaacagt ctgagagccg aggacacggc tgtgtattac tgtgcaagag 420

acacagtgag gggaagtcaa tgtgagccca gacacaaacc tcgctgcagg ggcatctgag 480

accacgaggg ggtgtcctgg gccctgtgaa ctgggctgct ctccgtggca gcggctggtg 540

gtgctaaagg ctgattttct ctcagcatct ggggctgatt catcaagttt cctcagagaa 600

cctttcagat ttacaattct gtacttacgt ttaatgtctc tgaatgtgac actttccttc 660

cctggtgtgt ctttgttttt gtgacaagag gacacattct cacctccaca gaagcccgag 720

tgtcactttg gggacagaaa tgaccctgcc ctggtcacca gaatcagagt cccgaggaag 780

cccaggagga cctgggaagt gtttttcaat cagactcagg gcaggcgtct ccgtgggaat 840

ctctgattgg aacaggcttt gggattcaga ttgggaccaa gagggaggct cacccagggc 900

cagggtcctt agaatcctga cagttttcac agtaacccca tcgtccttta aaactgaaca 960

tctaactcag aactgaccca tttggtcctt tctctgtaat ccattttcct tttctctagg 1020

cttcattctt acacttccct tttcaccttc attctgaaaa tggagggtgt gcttcctgtg 1080

gtctaaacca caggcctcag atgcattacc tggaactcag gtgtggctct ggctatggct 1140

cctgtggacc tggcaggctg agggatcttt ctcattccct ggtgcctgca tgcccctgct 1200

gtcttctatg cgtggatgca tttgggaaat gcaagtggac actcatagtc gtttcctcaa 1260

atgggatact gttgtagagc tgatcttgtg cttctcaccc tgtcacagag cccccactct 1320

cacttgtgga tttttgggag agctgaggat ggacacttta ttgggctgtg agctctgcat 1380

gatggcaatc gtgaggtctg ggtgggcaca gccagagcca atggagctgg ccaaaaggaa 1440

agacaggacg gaattcctgg gaagtcctac agctgctgtc taccatagag ttccattgtc 1500

ttctcctctg ctaggattaa accagaccga ccaagttcat ctaggacaat cttcctgacc 1560

tagagaagtg atgacaggct ttaataacac ctgtattata catcagagca acacctagat 1620

tagtgtttga ttgaataatt gagactatgt cctagtcaag gtgacacaca aaatcaatta 1680

ttaccatgat taatatttta tattgattaa tattttaata ctaatattaa tcaatgcaat 1740

attgatttaa tattaattaa tattttatat ttgacaatag aatcagactc atgttataaa 1800

taattttgca aatatatgta tattattatt ggtcttctga gcataaatct ccattagccg 1860

attaagtgtg catgcattgg tcgaaggagg gcatccaccc ttttgaggga aaatgcatgg 1920

agggtaaatg aggctgggaa gctgatggca tatgatggaa gcctgtccct gagtgaagga 1980

gagagggagg caggattggg tggaactttc ctacatttct gtgctgtgca aagaaactcc 2040

agattatcac tgagtctcct gcaagtcaaa gttgcccctc aggaaacccc catcactccc 2100

agcaatgact ctgccccaga tgagtgcaag gctcactcta ttcctgagaa aggagcacag 2160

gatatgggat ttagcatgag ccacgtcatg gacgtcagag agcaggagct gggtgcatga 2220

tccaagtgca ctgttctgcc tgtaggtgga gagaggaagg tgcattccca gagctaacac 2280

actgtggatt tttatacaga atacacgttt tcacctgatt tattggaagg catatgaaaa 2340

aatgtgcagt ccacaaacaa ttgtaatttc caaatttacc acaattgcat tatttcttca 2400

ttctgcatga tgtcctggaa cagagttaca tttttcatgg gtattgtttc atggattgag 2460

gacatgaatt tcacacctgc ctttttagcc atgtgcagag accttgagta gagcatgtct 2520

ggacctcatc tacactatct ctcatgcccc aagggaaaga gtggagactt gactgactgc 2580

aaactttggg gtgggagctg ctcctgcaca cctcagaggc tgcagagacc ccaagtgcag 2640

ttttattgag ttggggtgtc tttctatgtg ggggaattat gggctgcccc actcctggtg 2700

attgctggtc agctctaaag acgggttcag aatgaggtca cctggtaacc tttctcactg 2760

cagctccatt atgtaaatta ccactaccag attccaggta ataatgcaag ctctattttt 2820

gcgacctcat tttcttatct tttttgttca taagtttggt tatagaaaat aagtcccttc 2880

cccttatgag cctgtaaatt cagaagcaag ttacaagcat tgggtaaaca cacccattac 2940

aaatgacaga aattgcccat aacaaaaagg ccccatgcaa gtccaaaatc cagcagggca 3000

gtcaaatcat agagctccac aatgatcacc tgtaactccc cttctcacat ccgggacatg 3060

ttgatgcaat aggtgagttc cgatggtctt gggcagctcc acccctgtgg ctttgcaggg 3120

tacagcctcc ctcctggctg ctttcacagg ctggtgttca gtgtctgtgg attttccagg 3180

cacaaagtgc aaactgtcag tggatctacc attccagggt ctggaggata gcagccctct 3240

tctcacagct ccactaggca gtgccccagt aaggactctg tgtgggagct ctggccctat 3300

atttcctttc ctccttgccc tagcagaggt tctccatgaa tgatccacca ctgcagccaa 3360

cttatgactg gacatccagg tgttttcata catcttctga aatctaggca gaggttctca 3420

aacaccaatt cttagcactc tgtcgataat ggttcatttt tttggcctgt tcattactgg 3480

tatttttcaa aggaatctca cttgaatctt tactcttttg cattttgtct ccatgacaat 3540

gttgggaagt tttacctcca ccatcataac atgatctagt gatctcacac atttgtggca 3600

aacaatacct acaaattcag aagctctttg cttttctttc catgaaatat aattctttct 3660

gttctgtgta taagcatatc ttagcaactc tgtgcacacc cacatagatg tccacaagcc 3720

tatgaattat tctctgtaaa taaaaattta tatcaatttc cctcaatgtt cataattctc 3780

ctgagggtga ggaagctcct tctcgatctg ttcaaacaaa atgcccagaa accatctggt 3840

aggtaaggag ttcacctggc tctggtgtgg ggtctgtctc tttccctctg ttgtcacaca 3900

ggtcagccca gttgttcagg tcctaagaag aaagcccagg tttgtcctga ttttaaaaca 3960

catcaaactt ctgatgactc tcctgttacc cacatccatg gagatagatt atttattata 4020

taattcacca aactaatgtc aaatgcccaa gttgcaatac cacacatcct agggtatgtt 4080

catgcaattc aatggaggag aaagtctttc agagacagat ggatctgaaa tgataaatat 4140

gtgggtaagg actctgggct tgagtatcat tgtccagcca tgtttcacaa gtgtgtcctg 4200

tcagggaagg acaagagttc cttgtgttct cagagggaag gggtcacaga gttcctctct 4260

ggttcccagg aaagataatc gcactaatct tcatgatctt catgagacta tcctccagtg 4320

ctgacctgtt atagagtttt tgtctgaagt tctcactgca atccccaatc tacatatttt 4380

caatcagaag tgtttagagg ccaggacata tcttcacggt cacacattga gaaggatgta 4440

gatatgtccc actaccttct cctgagatct cagacagaat cccagatttc aaaaggacac 4500

agaaggacag ctctcaggtg cttttaaaaa atgacccact tccagggaca gggagcttcc 4560

ctataaccat ggtggatgtt ctgaactaca ataaacattg gatggatcca ggattgtttg 4620

aagtcactgt cattattaca ttcagctgct gtttcaatgt gtctgaagta gtaaatgaca 4680

atttagatga caatttatat gaatcttcaa gggtagaaca atattgacca tattccaaaa 4740

tctgtccttg atccatgatc acactcatct cccagaccag gtccttcagc acgtctcttt 4800

acctgaaaga agaggactct gggcttggag aggggagacc ccaagaagac aactgagttc 4860

tcaaagggca cagccagcat cctactccca gggcgagccc aaaagactgg ggcctccctc 4920

ctcctttttc acctctccgt acaaaggcac cacccacatg caaatcctta cttaagcacc 4980

cacaggaaac caccacacat ttccttaaat tcaggttcca gctcacatgg gaaatacttt 5040

ctgagagtcc tggacctcct gtgcaagaac atgaaacacc tgtggttctt cctcctcctg 5100

gtggcagctc ccagatgtga gtgtctcagg gatccagaca tgggggtatg ggaggtgcct 5160

ctgatcccag ggctcactgt gggtctctct gttcacaggg gtcctgtccc aggtgcagct 5220

gcaggagtcg ggcccaggac tggtgaagcc ttcggagacc ctgtccctca cctgcactgt 5280

ctctggtggc tccgtcagca gtggtagtta ctactggagc tggatccggc agcccccagg 5340

gaagggactg gagtggattg ggtatatcta ttacagtggg agcaccaact acaacccctc 5400

cctcaagagt cgagtcacca tatcagtaga cacgtccaag aaccagttct ccctgaagct 5460

gagctctgtg accgctgcgg acacggccgt gtattactgt gcgagagaca cagtgagggg 5520

aggtgagtgt gagcccagac acaaacctcc ctgcatggac gcggagggga ccggcgcagg 5580

tgctgctcag gaccagcagg tggcgcgcgg ggcccccaga gcatgaggcc gggtcaggag 5640

caggtgcagg gagggcgggg cttcctcatc tgctcagtgg tctccgtcct cgccagcacc 5700

tcgctgtcac cagggctcct ctttctttat tatctgtggt tctgcttcct cacattcttg 5760

tgccaggaaa gaaacgagga agacaaattt tcgtctatag ttgaagcttc accaattact 5820

aggaacttgc ctacaagttc ctgcatgacc cattataact tatcgattaa aaaatatata 5880

ttctaatgct tctcaccatc tcttgatttg tatcatcaac tgaattgtac cctctttgaa 5940

attcatatga tgaaacctta aattcaatgg atctatattg gaattttaat gaaataatta 6000

aggttaaatg tggtcataat tgtaagaccc taatgcaata gacgtgttgt ctttataaga 6060

agaggaagag acaccagaga cctctcactt ttcacgtgca ggcagagaag aggccatgtg 6120

gagacatagt gcactagaag gtggcccagt gcaagccagg aagaagccgc gccaagaacc 6180

agccctgcca gcacactaat cttcaacatt cagactgcag aattttaaga aaatcaatat 6240

ttgttgttta agccacccac tcctgttgtc ttcttatgaa gatccagaca gactaatacc 6300

acataactct gttagtgctg tcccctggat ggagaattag cctcctgagg ctgggcacat 6360

ctctgattga gtctctaggt ttcttggatg accatttgga gttgatgcct gaaggtgaga 6420

agagacaaac cgg 6433

<210> 2

<211> 8048

<212> DNA

<213> artificial sequence

<400> 2

tctgattgag tctctaggtt tcttggatga ccatttggag ttgatgcctg aaggtgagaa 60

gagacaaacc ggcccagttg ttcacgtcct aacaagaaag cccaggtttg ttctgatttt 120

aaaacacttc aaacttctga tgactctcct gttacccaca tccatggaga tagattattt 180

attatataat tcaccaaact aatgtcaaat gtccaagttg caataccaca catcctaggg 240

tatgttcatg caattcaatg gaggagaaag tctttcagag acagatggat ctgaaatgat 300

aaatatgtgg gtaaggactc tggacttgag tgtcattgtc cagccatgtt tcacaagtgt 360

gtcctgtcag ggaaggatca gagttccttg tgctctcaga gggaaggggt cacagagttc 420

ctctctggtt cccaggaaag gtaatcgcac taatcttcat gatcttcatg agactatcct 480

ccagtgctga cctgttatag agtttttgtc tgaagttctc actgcaatcc ccaatctaca 540

tattttcaat cagaagtgtt tagaggccag gacacatctt caaggtcaca cattgagaag 600

gatgtagata tgtcccacta ccttctcctg agatctcaga cagaatccca gatttcaaaa 660

ggacacagaa ggacagctct caggtgcttt taaaaaatga cccacttcca gggacaggga 720

gcttccctat aaccatggtg gatgttctga actacaataa acattggatg gatccaggat 780

tgtttgaagt cactgtcatt attacattca gctgctgttt caatgtgtct gaagtagtaa 840

atgacaattt agatgacaat ttatatgaat cttcaagggt agaacaatat tgaccatatt 900

ccaaaatctg tccttgatcc atgatcacac tcatctccca gaccaggtcc ttcagcacgt 960

ctctttacct gaaagaagag gactctgggc ttggagaggg gagaccccaa gaagacaact 1020

gagttctcaa agggcacagc cagcatccta ctcccagggc gagcccaaaa gactggggcc 1080

tccctcctcc tttttcacct ctccatacaa aggcaccacc cacatgcaaa tcctcactta 1140

agcacccaca ggaaaccacc acacatttcc ttaaattcag gttccagctc acatgggaaa 1200

tactttctga gagtcctgga cctcctgtgc aagaacatga aacatctgtg gttcttcctt 1260

ctcctggtgg cagctcccag atgtgagtat ctcagggatc cagacatggg gatatgggag 1320

gtgcctctga tcccagggct cactgtgggt ctctctgttc acaggggtcc tgtcccaggt 1380

gcagctgcag gagtcgggcc caggactggt gaagccttcg gagaccctgt ccctcacctg 1440

cactgtctct ggtggctcca tcagtagtta ctactggagc tggatccggc agcccccagg 1500

gaagggactg gagtggattg ggtatatcta ttacagtggg agcaccaact acaacccctc 1560

cctcaagagt cgagtcacca tatcagtaga cacgtccaag aaccagttct ccctgaagct 1620

gagctctgtg accgctgcgg acacggccgt gtattactgt gcgagagaca cagtgagggg 1680

aggtgagtgt gagcccagac aaaaacctcc gtgcagggag gcggagggga ccggcgcagg 1740

tgctgctcag cgccagcagg gggcgcgcgg ggcccacaga gcaggaggcc cggtcaggag 1800

caggtgcagg gagggcgggg cttcctcatc tgctcagtgg tctccctcct cgccagcacc 1860

tcagctgtcc ccaggggtcc tctttcttta ttatctgtgg ttctgcttcc tcacattctt 1920

gtgccaagaa agaaatgagg aagacaaatt ttcgtctgta gttgaagttt caccaattac 1980

taggaacttt cctagaagtt cctgcatggc ccattatagc ttactgatta aaaaatatat 2040

attctaacgc ttctcagcat ctcttgattt gtgtcatcaa ctgaattgtg ccctctttga 2100

aattcatatg cagaaacctt aaattcaatt gatgtatatt ggaattttaa tgaaataatt 2160

aaggttaaat gtggtcataa gtgtaagact ctaattcaac agacgtgtcg tctttataag 2220

aagaggaaga gacaccagag acctctcact tttcacgtgc aggcagagaa gaggccatgt 2280

ggagacgtaa tgcactagaa ggtggcccag tgcaagccag gaagaagcct caccaagaac 2340

caaccctgcc agaacattga tcttcaacat tcagactgca gaattttaag aaaatcaata 2400

tttgttgttt aagccaccca ctcctgttgt cttcttatga agatccagac agactaatac 2460

cacataactc tgttagcgct gtcccctgga tgcagaatca gcccgctggg gctgggcaca 2520

tctctcagat ttccacataa agtaggcaaa aaatagtagt tctgatataa aaatttgtca 2580

tgtccctgtt ggccaatttc tgggcaaggt cttttaaaga agccctgggg gctttgtcac 2640

aaaagttgcc ttttatcatt tattaggaca taactgatga acaatgagta ccagttggat 2700

ggagactgac cactgaccat cttctgctgt ctcctaagta tgccacagaa aaccacacca 2760

acattactct atgtcttcaa ctttctaaat ttgcactgat tggtatttaa ggcaggccca 2820

gcgttgaata actcctttag tttttgcttc tctgggaaag gtcttatcta tcctggcctt 2880

ggtcttcaag tttcagcaat tctgggaagc caaggacgcc tctatctcct cctccatgct 2940

ctgcaactca cctgagaaca gctttctcat tggaatgtct tctgtttaag gaataagagt 3000

ccctgtttca ggcttgggtg cctgagtaca cctactggat ccagcccagg attggagaaa 3060

ctttccagaa cacatcacct gagaaatgac cagtcacact gttacacttt cacaatttcc 3120

gcttcctcat gagaaaatta aaattgcaga gactttttca taagcgttgt gccatgtcct 3180

ttcttgtttt cttgcctgtt catttatgtc agaccaggtg ccacatctat gtaatcaggt 3240

tagaatcctg cctccagtaa cacatgaaaa ggacctatgg ttgtactttt ggtctttgct 3300

ccaaagtgta aagattacaa aagtcatcac cctcattctt atgccaagag tcatctgcac 3360

aatctgatct tcaatacatt ttagaatcca tcaaatgaat gaaattccat tttttaaatt 3420

accaccccaa aaactagaga gatgggcatg tccagaatag cagttgatgg ttgcttaact 3480

ggaagagaag tttcagaagc cacaagctgt tgaaggcact tacgtggtta gcactataga 3540

cgtctgcaag acagatgtgg actagggtga aatgacagtt ccagagggcc gcactctcct 3600

cagtcttctg gaatttccct ctagaaatct ccagaatcta aaaaatacaa tccaaatatg 3660

tttcctatgg gtcataactg gggaagttta attactgaaa aatatatcag gagccttctc 3720

caaaagatcc tacagggaag aacttttcca gaacctcata ctatgtgaag ggaagaaaaa 3780

tctgcccatt ccagatccct ccccacttcc tccattatta tacaaatgag taagtttagc 3840

caatagggta agatgtaagc aaatagtcca gggaaactga agccacaaaa aggagtaaag 3900

atgaaaattc agcttttccc ctggagatgc ctggtcaagg tcacagccca gaaaaggaat 3960

ctgattgagt ctctaggttt cttggatgac catttggagt tgatgcctga aggtgagaag 4020

agacaaaccg gttattagaa gacatgtatc aaaaccaaac aaggtggtaa ggacagtttg 4080

aaaaaaaatt ccaaggctgc tgacacaccc agataactgg tggctgtagt tatgcctgct 4140

aagatttggg tgcatggggc ttggctttcg ttacctccct tggacttatt ttcccaaaca 4200

aagaaacctc cgggttaggg ggaccctatt tattccagtc acctggcatg atttgcagga 4260

taattgctca gaattaaaat attcgtccag atgtttatat agcccatgcc tgtgtttctt 4320

ctgagctgca gccagagatc attggttggt tcacagcgat aagcagagtt agtctaaaat 4380

ggaggcaaat acttaaaact tatttcttct ctcagttaat ggattctata gagaaaagta 4440

gctactcggc atgggaatgt aaaaaaatga gtaaactatg atcttattct gaactcatta 4500

acaacaaacc tgaaaaacca attgaagaga ctgtaattta aagacaagtg tatgatatgt 4560

tttgaaacat aatttttctc tctccagttc tgatttttgt cagaaactaa tcattatagg 4620

actgagtgat ttgcaaaata aactttagtc ttatggttgg tctgatcatt tgcataaagc 4680

gaagccataa taattaataa taattctgta ggaaaagcct gcaagcacga ggagcttcac 4740

agtctaacac tatgagcaca tgcatcctcc agcaactcac tgaatatttt caagtcagct 4800

ggttcttagc ttaaataaca tccagttggt atctgtccca ggaacactaa tatatggttc 4860

tctctgcagg cccctttctc cacagattaa gggttttttt ttttctctgt aatatcaact 4920

cagatatgtt gaatgctttt tccttattag tggtttttca ggtttgttgt taatgatttc 4980

agaataagat cattgtttac tcattttttt taaattcccg tgccgagtag ctacttttct 5040

ctatagaatc cattaactgg gagaaaaaat aacattttct tatgggtgaa caattaaata 5100

gtttgacata tatttatgta ctggtatata atgcagcttg aaatcaaggc atgcctcaat 5160

cataaaaatc atggctaaat tctcaaagaa ttgtgctgag tgaaagaagc taaggaatta 5220

agagtaaatt ttatataatt cattgtagaa atattagaag atgccactac cataaattaa 5280

aatgaagaag acttaaattt ttctgagaaa atggtgttgg gaatgatgcg gatgtgattt 5340

aagtttcaga ggaataagaa aaagatttag ggattaattt aattattcaa aagttgattg 5400

aagtgccgag tgaatggctg caaacatagc tctacatttt tcaaatcatt ccctataaat 5460

ttgaattaat tatttatttt tatacttgaa taaagcaata acaaagaaat aaatgaatat 5520

ttttgctaaa atggagcaat aaaaagactg atattgacag aagaaatatg actgacttct 5580

gaaaatacac acacatgagc cgtggttctc tctacatatt tagataaatt acagaaagtt 5640

gtcataactg atggggaatc ctgcagactt cactaggcat agtccacact gccctggagt 5700

tgtctcaggg gagctgcctc ctccagtggt tagagcacag gcccaggtaa taggactcat 5760

ttttttagat gtgtaatttt agacacactg cacaactgct gtgttctctg tgcaaattat 5820

ctcctgtaaa atgtaacatt gaaacctgcc ttaaatatat tgtgtaaata tgtaaaaata 5880

aaatcagatt gtgagagcta aatgctaatc aaggcgcaat cacgtaatat acaattatat 5940

tttcctgaat gatggaatta ataccaatct cccccaggac acttcatctg cacggagccc 6000

ggcctctcct cagatgtccc accccagagc ttgctatata gtcggggaca tgcaaatagg 6060

gccctccctc tgctgatgaa aaccagccca gctgaccctg cagctctggg agaggagccc 6120

agcactggga ttccgaggtg tttccattcg gtgatcagca ctgaacacag aggactcacc 6180

atggagtttt ggctgagctg ggttttcctt gttgctattt caaaaggtga ttcatggaga 6240

actagagata tcgagtgtga gtgaacacga gtgagagaaa cagtggatat gtgtggcagt 6300

ttctaaccaa tgtctctgtg tttgcaggtg tccagtgtga ggtgcagctg gtggagactg 6360

gaggaggctt gatccagcct ggggggtccc tgagactctc ctgtgcagcc tctgggttca 6420

ccgtcagtag caactacatg agctgggtcc gccaggctcc agggaagggg ctggagtggg 6480

tctcagttat ttatagcggt ggtagcacat actacgcaga ctccgtgaag ggccgattca 6540

ccatctccag agacaattcc aagaacacgc tgtatcttca aatgaacagc ctgagagccg 6600

aggacacggc cgtgtattac tgtgcgagag acacagtgag gggaggccat tgtgcgccca 6660

gacacaaacc tccctgcagg aacgctgggg aaatcagcgg cagggggcgc tcaggagcca 6720

ctgatcagag tcagccccgg aggcaggtgc agatggaggc tgatttcctg tcaggatgtg 6780

ggactctgtc ttcttctgac ggttccccag ggaacctctc taagtttagc attctgtgcc 6840

tatgaacgtc ttctctaagt atttgaaaga gattatttta atatgaagag cagttctcac 6900

tcgcacaaaa tgtggattga tgcttactgg gatgaaaagt cctcaaacat ggtcaccacg 6960

atcagagtct gagtgagctc agggcttcct gctgagtctc ctcctatcag accaaggaca 7020

gggacctcag tgaggttccc cgtctagaac agtctttatg gatactgatt gtgggcggca 7080

agccacccag gtgccgacgc aagagaccga ggacacgagc tgttccagta caataaaata 7140

taaaacaaga atagttatac cagatataga tcttagatat gattatatat gaatatcatt 7200

aatcattagt tggtagcaat tactctttat tccaatatta taataatcct cactctacaa 7260

tcataaccta ggaaaagcca ggccatacag agataggagc tgaggggaca tagtgagaag 7320

tgaccagaag acaagagtgc gagccttctg ttatgcctgg acagggcgac cagagggctc 7380

cttggtctag cagtaatgcc agcatctggg aagacgcctg ttgccaagcg gaccatggtc 7440

tagtggtaga ctcagtgtca aggaaaaaca cctgctactt agcagaccag gaaagggagt 7500

ctccctttcc ccggggagtt tagagaagac tctgctcctc cacctcctgt ggagggcctg 7560

atatcagtca gacccgcccg cacttatccg gaggcctaac agtctccctg tgatgctgtg 7620

cttcagtggc cacactccta gtcctccttc gtgttccatc ctgtacacct ggctctgcct 7680

tctagatagc agtagcaaat cagtgaaagt actaacagtc tctgataagc agaaataata 7740

ttgtaagctg tttctctcct tctcctctct ctctctgcct cagctgccag gcaggaaagg 7800

gtcccctgtc cagtggacac gtgacccatg tgaccttacc tatcattgga gatggctcac 7860

actccttacc ctgtcccttt gtcttatatc caattaatat cagcgcagcc tggcattcag 7920

ggccactact agtctccgca tcttggtggt agtggtcccc cgggcccagc tgtcttttct 7980

tttatctctt tgtcttgtgt ctttatttct atgctctctc gtctccgcac acggggagaa 8040

acccactg 8048

<210> 3

<211> 6000

<212> DNA

<213> artificial sequence

<400> 3

gtcttttctt ttatctcttt gtcttgtgtc tttatttcta tgctctctcg tctccgcaca 60

cggggagaaa cccactgacc ctgtggggct ggtccctaca ctgatcacag acaatagagg 120

gtaggccagg atcagtgtca tgtaggacat cacaggtttc acctctgaac cttttcctga 180

cactaaatat gcaaatcagc atcagcactg atctggtgat tcttttgttc ctaatccatt 240

taattccttt ttcagtcgtt gttttcattt ttccgtttgc ttttcctgct ttctgcaaaa 300

ggaagatttt tccctgtggt caaaattccg gacctcaagc cctttcctga cgctcaggtg 360

ggtctcaggc tgtggctgct gcagtcacgc gggagaggct ggtgggactt tcttcactcc 420

tcgtcactca gggccctcca ctgtgttgca tggagactta tctggaaatg caagttgcga 480

ctgagaactg aaggggacaa gcttgtttgg ttaacatggg atgtggatgt gtttctaatt 540

ttgttctgat aaactttcac agagtaactt tctgcactag tcatgtgagg aagaggatgt 600

gaacgttgtc agaataaaaa tagaacaact tgtgttataa tctttacagg tgaagctgga 660

gaaggtcatg aatagagggt tctcatgcac acatccctga taacaagaac taccataaaa 720

ttactctgca caaccacaac tttcaacaaa ggctaccaca acaataagag aattaatatt 780

gtgaggatat ctgccctgca actcccagta caatcttaaa ctgattccac ccttgttatt 840

aattcttcta cccccaggat aattgcctca gaacagctca tgtaagtcct ctcatttatc 900

ctttaaaaca acctttacca acctttacta acctgacttc ctttacctac ctaaatatgc 960

agaaatcact tccaaaacat tcctcttatc tctcttaata ttgtgattat tttattagtt 1020

aaaatgtatt tttgtttgtg tttgtctgtg tctctgtgtg tgtagcctgg gtgcacgcta 1080

tactagctgt tttcataatg acttatggtg aatgcctatt agtgctcaag aggccggcgt 1140

ctgagcactg gaggtcaatt gtgcaggtgt cacacacatc tgtgatgggc ccaataaaca 1200

gccctgggca caagcactca ggtgagcttc cctggtggac aatgcttcac acatgttgtc 1260

atgcatcact gctgggagaa ctggggacaa gagactcctc agggaaagaa cacctgggag 1320

ctcatgcctg gattctcagg gccttctccc tggtgccttt gctcatttta attcatattc 1380

attcgctata ataaagctgc acccatgaga ataacagctt ttcttgtgtc ctttatttgt 1440

actgattaag gggcctgagg atggtcttgg gacactcaac acaattacat cagagttgga 1500

gaatgctaga aagttcctga ttgctgacgc atggctagag gattttttat aacatcaaag 1560

gatgagaaag tgcgggataa aagacattca atgcccagat ggctacagaa tcatatggca 1620

tgagatggca tgtacactcc aataaggagc aggaagtgaa agtaatccat ggaatttaga 1680

aacaacagat acagcttcct aggagttgtt ccctgaaaaa ggaaaagtaa aaataatgag 1740

gaacaacccg aatatgcaat tcatatctgt gatagctaaa ataatagtaa aaagatgcac 1800

atccagccct gcagtgtgcc ctgtgagccg aggctgctag actcccattc accaccaaag 1860

ggtacagttg tccagaaaca acacacaaca cataagagtg acacagaagg tagcccagag 1920

tttgggctgg ggagaggaat ccactagaca actataacaa ggaggacagc atggaaagtg 1980

gcatcatttt gtgatatgat tgatatcatt aatttatatc atcggattgt gaacaaagtt 2040

agccaatgga ctatgagagt gactgacgta gcagcttctc tggcttcgtg tgctgcagat 2100

gagaggagaa tgtttgggag gatgctggac ccacagctca caacagacat atgacagacg 2160

tagctgatcc caacctcaag taggatgttc ttgatgagac agcttccctg gtgcactgga 2220

tatcagatcc tgtatagttt cagaacttgt gtagtttgtg ttgctttggc tttttctctg 2280

gatcttttag gtgctaatga aatgagagtg cttcacaaaa aaatggggca gacaaagggg 2340

atagttaagg gcctcctcca agcaatgtgg atatcataaa ataaatgtta aaaaatgaaa 2400

gaactaaaca agaaggtgct ggggttgaca caaaggactt aaaacagcac taccaggagt 2460

tgggtgcagc aatgggcatc ccttcaggtc catcagcaag tcagagacct aaacaaatat 2520

tccctattca tgttgaattg gcagagtgta aaaatctaga agacaaaatg gccaggaaaa 2580

atgtgcgatt ttgcatgggt gaaggtctgg caagttaatc atcttgagga ctgttgaggt 2640

cctgaggtgg tcctgccctt gcttgatgcc caggccgtag tccaactcac acgaaattgc 2700

cagggaatag acagtagttt ttttagtagt tcttgttatc aggcataagt gcatttgaat 2760

tttctcttca tggcctttcc tggcactatt tctcattttt tttaacacac atagtttcaa 2820

ctagatttat caccttcaca gggtcacaga gaagggtgga agaagggagg ccctgtatgg 2880

gtctcgaaga aacatggaaa agagtggaga gggacaatag cagggtgtaa ggaattattg 2940

agaccttact ctgcccctcc caggaggctc aggccagcct ttttctgcat ttgaggttct 3000

gggttataaa cgctgtagac tcctcccttc agggcagggt gacaactatg caaatgcaag 3060

tgggggcctc cccacttaaa cccagggctc ccctccacag tgagtctccc tcactgccca 3120

gctgggatct cagggcttca ttttctgtcc tccaccatca tggggtcaac cgccatcctc 3180

gccctcctcc tggctgttct ccaaggtcag tcctgccgag ggcttgaggt cacagaggag 3240

aacgggtgga aaggagcccc tgattcaaat tttgtgtctc ccccacagga gtctgtgccg 3300

aggtgcagct ggtgcagtct ggagcagagg tgaaaaagcc cggggagtct ctgaagatct 3360

cctgtaaggg ttctggatac agctttacca gctactggat cggctgggtg cgccagatgc 3420

ccgggaaagg cctggagtgg atggggatca tctatcctgg tgactctgat accagataca 3480

gcccgtcctt ccaaggccag gtcaccatct cagccgacaa gtccatcagc accgcctacc 3540

tgcagtggag cagcctgaag gcctcggaca ccgccatgta ttactgtgcg agacacacag 3600

tgagagaaac cagccccgag cccgtctaaa accctccaca ccgcaggtgc agaatgagct 3660

gctagagact cactccccag gggcctctct attcatccgg ggaggaaaca ctggctgttt 3720

gtgtcctcag gagcaagaac cagagaacaa tgtgggaggg ttcccagccc ctaaggcaac 3780

tgtatagggg acctgaccat gggaggtgga ttctctgacg gggctcttgt gtgttctaca 3840

aggttgttca tggtgtatat tagatggtta acatcaaaag gctgcctaac aggcacctct 3900

ccaatatgac agtattttaa ttagtgaaaa ttttacacag ttcatcattg cttgcttgcc 3960

ttcctccctc ctgtccactc tcactcactc cttcttttat tttctactta attttacaaa 4020

atcatttaac ccctttttga actattaata ggttatcttt gtttggtgat tgttttcctt 4080

tcaataatat gtactgaata attcatcttt gtgccaattc ataagtattc tggtgtaata 4140

aagacttctt tcataaaaat tggataaatt aaaataaaga taaattttta aaaacatacg 4200

atctatcaaa actgaaccat aaagaaataa aaactctggg ttgggtgtgt ttgctcattc 4260

ctgtaatccc agcactttgg gaggccatgg ccggtggatc acctgaggtc aggagttcga 4320

tatcggtctg gcaaacgtgg agaaacgctg tctccactaa aaatacaaaa attagctgga 4380

catggtggtg ctcgcctgta gtcccagata cttgggagcc taaggccaga gaagagattg 4440

aacccgggag gcagaggttg aaataagccg aaatctagcc actgcattcc agactgggca 4500

acagagtgag actccatccc gaaaaaaaaa actgaacaga cctatgagta aagagattga 4560

gtcagtgatt tttcaaacat ctcaaatcaa agaaaagtca agaacttcat ggcttcacta 4620

ctgaatttta tcaaaaattt aaaaaaaaac tagaatctct atacaaattt ccaacaaaat 4680

aaagaggaaa aaatacatgc aagcttattt tggaaggtcc tatttccaaa gcaaggaaaa 4740

gacactccaa ataaataaaa ttacaggcta atatccctga ttatctagtt tcaaaaactc 4800

tcaagggtgg tgagaaacca aattcaacag cacattaaca acagaattca ccatgatcag 4860

gtgtggttta tctctaggaa gcaatgaagt ttcaacctgc agaaataaat gtgatatatc 4920

aaatgaaaat attgaaggac taaaaccata tgcaccatat gtccatgtca atagatgcag 4980

aaagagcctc tgtcgaaatc cactacactc taatttttaa aaatctgaac atattatgca 5040

taaaacatat atacctcaac ataataaaga ccacatatca caagcccaca tctaacatca 5100

tacacagtga tgaaaatttt attttcctct aagactagaa actagacaag atgcttcact 5160

atcaccaata ttattaaaca cagcactggg tggtctagac aaaacaggcc agaagaaaaa 5220

aatagaagtc atccatatag taatgaataa atataaaata tatttttaca tattacatgc 5280

tcttatttat acaaagcctt aaacactcca ccaaaaaaga ttgaaactaa tgaagaaatt 5340

caataaagtt gcagaatcca aaatcaaact tacatttcaa gatggcaggt tagaggcatt 5400

gctagtatac ctcttccact tggaaggaca aactgatgtg gagagatgaa catcgcggac 5460

ttattttcaa gaagcaatgc aggaactgaa cagaaacact gaaataatct acaaactttc 5520

tgaaaaagca ggaagctgca gcctacactg tgagtcaggt gaagggctgg gagtccccag 5580

catttgaggg agaacagcca aaaatttcag ccagtggtcc caagttgaaa gtagctctca 5640

acaggggtgt gtaatataac ctagggttgg gacgaactcc cttggccagg gcctgggtag 5700

ggaagtgtta aaagtgggct ctgcaagttt aggagccatg ggtgcaggag ctggtgccct 5760

gctttgcagc agacaggaag gggcatggca tgaaacccat ggctgcagtc tccatgggga 5820

cagcctatga ctcctggcat ttgcaggtat tgatcacaga ttgactgaaa ctcatctcac 5880

tgctgccagt ggaacaccac gggagtggat caccaagtat gtgggaactg tgtggggcct 5940

accaccacct gccatactgt tgttgtaaaa cataagtaaa atatattatt catctatgta 6000

<210> 4

<211> 9783

<212> DNA

<213> artificial sequence

<400> 4

tcaccaagta tgtgggaact gtgtggggcc taccaccacc tgccatactg ttgttgtaaa 60

acataagtaa aatatattat tcatctatgt acatttccaa gctgtgtaga agaattttta 120

ataagaccca gagtaaaaaa agaatgcaaa tatgtagggg ccagccctac agggtctgtg 180

gatctttctc cccatgtgca gagatgagag atcgtagaaa taaaggcaca agacaaagag 240

atagaagaaa aaacagccgg acccagggga ccactaccac caagacacag actagaagtg 300

gccccgaatg cctggctccg ctgttattta ttggatacaa ggcaaaaggg gaagggtaag 360

gagtgtgagt catctgcaat gattgataag gtcatgtggg tcacgtgtcc accagacaga 420

gggcacttcc ctgtttggca gccgaggcgg agagagagag aggacagctt aggtcattat 480

ttattccatt ctcttctcag aaagatcaaa gactttaata ctttcactaa ttctgctact 540

gctatctaga gggcggagcc aggtgtacag agtggaacat gaaagtgaaa caggagtgtg 600

accgctgaag cacagcatca cagagagacg tttaggcctc tggagggctg cgggcaggtt 660

tgactgatgt caggccttcc acaagaggtg gtggagcaga gtcttctcta actcccccgg 720

ggaaagggag actccctttc caggtcttct aagtaatggg taatgggtgc cttcccaggc 780

actggcgcta ccgctagacc gaggagccct ctagtggccc tgtccgggcg tgacagaggc 840

tcacactcct gtcttctggt cacttctcac tgtgtccctt cagctcctat tgctgtatgg 900

cctggttttt cctaggttat aattgtagag caaggattat tataatgttg gaataaagag 960

taatgctaca gactgatgat taatgatatt catatataat catatctata acctatttct 1020

agtacaacta ttcttatttt acatattctc ttcattacac tggaacagct tgtgccctca 1080

gtctcttgcc tcagcacctg ggtggcttgc cgcccagaca aatattgtta agcttcttaa 1140

tagaaaaaca aattatggta aatgtgttca ctggaatact acccgtcatt tataataaat 1200

taatgcctga tacacagagc aacaaggtaa aatatctaag tatttatgtt gagtaaaata 1260

agctaaacaa ataagaatat atactatgta atttcatttt tataaattct gataaataaa 1320

aatgcatctg aagtaaaata atgaagataa gtagttgcct ggggtaatgg tagaagaagg 1380

gagggggaga ggaggaggaa tacagcagaa caaggggaaa tgttgagaag aattcacttg 1440

tccactttct tggtaatgat agcagttaca tcatttttat tagttgtaca ttttaaatat 1500

gtgaagttta ttatctttca attaagcctc ataaaatgtc ttacaagcaa acaaatggaa 1560

acttagacaa ggaaagagta atagaaagac agaaaaaata agttcaatgt cagaagtacc 1620

tgaaaattaa tgtgcctgga tcctagttct ctccatattt tcagaagagt gctggagggc 1680

agcaaaacca cacatgctct tattacggaa tgtgggttct gataaaaaca ctagacacat 1740

ccagctttgt cctggagttg gtttaggggg atgtcagaga cagtgatgaa gagcacaggg 1800

ccagataccg gggttcactc atcccagaca tgagctccga gacgcataca gagccccccc 1860

atgtgtgggt ttacttccac ttctgtaaat ggagaaaata ttgtctccta cagaacatag 1920

tttacatgaa tatttaaaat gaaatagggt gattagtgca aagtgtttat cacagcacaa 1980

tttcataata agacagcata ttttccaaat gcaatcattg ccagcaaact tctacagggc 2040

accgtcgtct tatctgggta cagcctactc ctcaagggtc ccaccctaga gcttgctata 2100

tagtaggaga tatgcaaata gggccctccc tctactgatg aaaaccaacc caaccctgac 2160

cctgcagctc tcagagaggt gccttagccc tggattccaa ggcatttcca cttggtgatc 2220

agcactgaac acagaggact caccatggag ttggggctgt gctgggtttt ccttgttgct 2280

attttagaag gtgattcatg gaaaactaga gagatttagt gtgtgtggat atgagtgaga 2340

gaaacagtgg atatgtgtgg cagtttctga ccttggtgtc tctttgtttg caggtgtcca 2400

gtgtgaggtg cagctggtgg agtctggggg aggcttggta cagcctggag ggtccctgag 2460

actctcctgt gcagcctctg gattcacctt cagtagttat gaaatgaact gggtccgcca 2520

ggctccaggg aaggggctgg agtgggtttc atacattagt agtagtggta gtaccatata 2580

ctacgcagac tctgtgaagg gccgattcac catctccaga gacaacgcca agaactcact 2640

gtatctgcaa atgaacagcc tgagagccga ggacacggct gtttattact gtgcgagaga 2700

cacagtgagg ggaagtcagt gtgagcccag acacaaacct ccctgcaggg gtcccttggg 2760

accaccaggg ggcgacagca cactgagcac ggggctgtct ccagggcagg tgcaggtgct 2820

gctgagggct ggcttcctgt gatggcctgg ggcggcctca ttgtcaaatt tccccgggga 2880

acttctccag atttacaatc ctgtactaat atttgatgtc tctaaatgca accttttttt 2940

tcctttttgt gtctgttttt tttttttaaa acaggaggac acatcctcac ctccacagaa 3000

gccacagtgt cactttgggg gcagaaataa tcctttcgtg gtcaacaggg tgagagtttt 3060

gaggaatccc agggaaacct ggggaatgtt ttccaattag actcagggca gagacctcca 3120

tgggaatctc tgattagaac aggctttgag ttctgatggg agccaagaga gaggctcacc 3180

cagggtcagg gttcttaaaa cctgatggtt ttcacagcaa tcccccttca tcttgtgaaa 3240

ctgggcacat ctgactcaga ctgattcagt tgaccctctt tctgctaatc cattttcctt 3300

cccagtagac ttgattctca cagatccctt tcttcttctc tttcctgaaa acagaggatg 3360

tgttttctgt agtctaaatt ccaaggctca ggtccgcagg agctgggtag gctgaggggt 3420

cttcctcact cactattgcc tggaaaatcc tgctgtcttc tgtgcatgga ggcatttgga 3480

aaatgaagca ggcattagtc atgaagggaa taatactagt tttctccaat gggatgttga 3540

tgtagagctg atcttatgct tctcacactg tcacaaagtt tggactctca cctgtgactt 3600

tgaggagagc ttgtgatacc ttatcttgtt ttaatatgaa tagactctcc cttagctcag 3660

gaagctggaa cagactccat ttggctcctt catttgtaag acatcaaggg ctcctcaccc 3720

acccccttcc tcaaggactt aacttgttta agctgactcc cagcatctca aagagtgcga 3780

ttaactgata aggtactgtg gcaagctgtg tccgcagttc ccaggaattt ggccaggtga 3840

tggtacccta aagcccctgc atttgtgtct ggcagataac acccagagcc cccacaccta 3900

tcatcttgtg atgaatttaa agcccctgca cctggaactg tttgcctgta accatttgtc 3960

ctttcaactt ttttgcctgt tttactcctg ttagaatgct acagttaggc tccccctccc 4020

ctctctaaac caaagtataa aagaaaatct agcaccttct tcggggctga gagaatttcc 4080

agcgttagcc atctctcagt tgccagctaa taaaggactc ctgaattcat ctcaaagtgc 4140

ggcgtttctc tctaactcgc tcggttacaa caagctgagg atggacactc cattgtgcag 4200

tgagctctgg gtgacagtaa tcgtagggtc tccccgggca gcctaaggtc aatactgctg 4260

gccgtcggga aagacaggct ggaattcctg ggaagacctg catctgccat ctaccacgga 4320

gtcctatggt cttctgttac actctctttg aatcagcacc acctagatta tctaaaacac 4380

tctttgtgac ttcatgactg ggaaaaaata atggcagtct ctactaacac ctgtgttaag 4440

ccatgggagc aacacctagg ctagtgtgtg attgagtagt tgagactgtg gtctagtcaa 4500

ggtgacacat aaaattgatt gttgccatta tgatatttta ttttatattt gacaatataa 4560

tcatgctcat attataaata tttctgttac attatttgtg tcagaggctt tggaaccaga 4620

acaacttcat cttgaataag gggtaggaaa aataagactg agacctgctg ggctacattc 4680

ccagtaagct aaggcgttct tagtcacagg atgagatagg aggtctgcac aagatccagg 4740

tcataaagac cttgctaata aagtttacag taaagaaact gggctgaagc ccaccaaaac 4800

caagatggtg acaaaagtga cctctgtttg tcctcattgc tcattatatg ctaagtatta 4860

tgctttaaca ttctaaaaga cactccccac agagccatga cagtttacaa atgccatagc 4920

aacatcagga agtttcccta gaaactaaag agtggaggaa acctcagctt tgggaattgc 4980

ccggggaatt catgaataat ttataaggaa aatgggtcaa attagccaat agggtaagaa 5040

tcctataatg ttccagccag aaaagggaaa gcatcaattt gacttctgga gactctgtat 5100

ataggtcaca ttccatagaa agaagagcac taaattatta agattcaatt gtaactacat 5160

atgatacttc cgggatgcac aaattaaaga tgaaagtgtg acaatgcaca ggctctgtca 5220

gagaaggaga acgtagggaa actgaaagag aatggcagag agtaagacaa ggacagaaga 5280

gcaatatgaa gcctctgaca tcaactctgt aaggacaagg tcttggcaac tcccgcattg 5340

accacagatt ctgttaccat gggccatttg cagggttcct agctaaggaa aaagaaaaaa 5400

aaaaaaactg catgcacttc ccaagtctcc acttgtatcc tgtttttctt agatctctag 5460

gacaaaaaaa atggcataaa cctagaccta gtgtcagtgt aggaggtact ttctttatag 5520

gcaagacact aggaagaagg gaaaatgtgt gttattggac taggagatac agagatggct 5580

ttaatctgaa tagatctaca cccgcaggta ttctcgaatg caacattcaa ctacaagagc 5640

ctaatgaaga aacacgaccc tccccaaacc cctgcaagct cttgtattca ctgtgtctcc 5700

actgatttag tgcacctgga gcttcagagc actggctccc tcctgtgtcc tagaatcttt 5760

tctttgggtc tttctgcaga attcaatagg attagttagg ctaatcaatt gtttcaagag 5820

atggtgttgg cagcatatat tgtcgctgga agagtattct aagccaggac acaggcactt 5880

tatgccggac taaagactct ggagaaaatg ttttgtgagc cctgacagaa acctccttga 5940

aaggtaaggg ctgggcagga ggggcactca ggagccacgc agcacacggt ccagccctag 6000

aacaggaggc tggggaggag gtttcctctc agggcctcgg ttttccttcg tcagaaaaaa 6060

aaaaatctaa aataaccgtt caacaagttg ctgatatgcc ttcaaatatc ctgctacaat 6120

ggaacaattc atataacttc aggcaatgag aactattttt taaatcgggc ttctaggatt 6180

ataagtatct taatagtgaa aatgtgaaga ataggtatga ttttactatt tcaatggata 6240

cagaattgtg ggagtcacta tattcctatg aacaaaaaat tcagatttca gtgttaagta 6300

atgttgccta cattgtgtga gtgacggggc agtggtggat ctgagagtgt ggtgggtgca 6360

cggacataat gattcagaaa gcaatatgga aagatgagta tctatggata cgaactgaaa 6420

gtatgtaaat acttcacaaa atactaataa acggagttga atataaaacc cataattatc 6480

caaaacacaa atttcttgga agttattttg ggaacatgat ttcataaaga actccaaact 6540

cttgtttcaa cttctgactc ctcgtttctg tgatataaga aaaccatttc caattatgca 6600

tctcagggca attctgtaaa cccagagcat ttctgctgaa gatcctgggg aatcaagaca 6660

ccgggcaggt gatggagaca ctgtctcagg tgcgcccaac gaatctcaga ggaacctgct 6720

ggagagtcac gtggaacatc tacagtcagt ttctcagagt caacagtgag ctgtgttggt 6780

gcctgagggg accatgatgg ggccaaggca cgtgctcagt gtcgtggaca gtgatggtcc 6840

agaaatgatc tagatggtct tgacgctaat gaaatatggg ttcagagtga ggagcataat 6900

ctgtggggac ttgttcttca gtgaaaggat cctgtccgca aacagaaatg gagcaggaca 6960

tgcatttctt caagcaggat tagggcttgg accatcagca tcccactcct gtgtggcaga 7020

tgggacatct atcttctttc tcaacctcga tcaggctttg aggtatgaaa taatctgtct 7080

catgaatatg caaataacct tagatctact gaggtaaata tggatacatc tgggccctga 7140

aagcatcatc caacaaccac atcccttctc tacagaagcc tctgagagga aagttcttca 7200

ccatggactg gacctggagg gtcttctgct tgctggctgt agctccaggt aaagggccaa 7260

ctggttccag ggctgaggaa gggatttttt ccagtttaga ggactgtcat tctctactgt 7320

gtcctctccg caggtgctca ctcccaggtg cagctggtgc agtctggggc tgaggtgaag 7380

aagcctgggg cctcagtgaa ggtttcctgc aaggcatctg gatacacctt caccagctac 7440

tatatgcact gggtgcgaca ggcccctgga caagggcttg agtggatggg aataatcaac 7500

cctagtggtg gtagcacaag ctacgcacag aagttccagg gcagagtcac catgaccagg 7560

gacacgtcca cgagcacagt ctacatggag ctgagcagcc tgagatctga ggacacggcc 7620

gtgtattact gtgcgagaga cacagtgtga gaaaccacat cctcagagtg tcagaaaccc 7680

tgagggagga gtcagctgtg ctgagctgag aaaatgacag gggttattca gtttaagact 7740

gtttagaaaa cgggttatat atttgagaac aaagaacaat agaaacacaa tcgaattgta 7800

agagaaatat tccattcaag agccaccaca taagccaaac tgacagagtg ggaaaggcca 7860

cactcagtaa agttgataca aacataccat aaaggtgcta ctatgaacaa gtttttgaat 7920

tagatgaata aatcatttgg agcaaggtta tttggtcata tgttaagagt aagcatgatt 7980

cttacaaagt gggaaaattg tctttcaaat gtttctgtca cttcttacca taaagttcat 8040

tttagaggtt ttaggattac agtgaaattg cacagaaggt gtgagaattc ccatgaatcc 8100

ctgccccgca cggacaccgc ctcctccact acagccatcc tgccccacag tcacaaataa 8160

gtcacaatgg atgaatctac aagaactctt ggttctttct ttttctggtg atcccctaat 8220

ataacaagcc taaattatct tggaacaccc aggtattttc aatggctttc tagaagtgat 8280

attagtcaga gggaaagtga gtgaggctat tactatttga gcactttctt ccaaaatcca 8340

caaaatatat gttaatttgg agtttttctt acttctggtt tacaatgtcc cttcccagag 8400

agtaagattt ttaagctttt agtgaggctg gaaaaaaaaa tttttaaaaa agagaaataa 8460

gctttcctgt attaggctga cttatcccag cggcagcaac aagcacagcc cagacccagg 8520

aaaagtctta ataatattat ctaatgtgct ctggagactc tttcagcact ccctcaacat 8580

agggagaaga aaaacaaatt ttcctttgtc ttatgatatg agtttataga ttcttgttct 8640

ctgtaactag taacttcaag tattctgttt tatctaagaa gcacaacgaa ggtcatgaga 8700

agcctgagca ggccagaact acagctgtct aggtaccgga gtgagtgtta tgagatcaac 8760

cagtgcaagg ctctttagaa caaaacctag ataacagaca tctgggttgc atagcaatgg 8820

tcatgtgtaa tcctgagtta tgaacctgtt acaatttgat taactgtctc tgtcctgcct 8880

ccgtatccct gcttttgtgc actctaagct tgcttcaagc tagcccaccc cattttggga 8940

agtgtgtata aaagtcaagc gctctctttg ttctgtgccc agtctttggt cattgagtct 9000

gctgggtctg ggtgtactca gtaataaaaa tatcctcctg tatacacccc aagatctctc 9060

tctggtcctc cagattctgc aacatttcag gcagattcac atctctaaaa ggccagcaag 9120

ttctggtcaa tcccataatg aaaatccttt aatgagactt ggcaaacgtg acaataagag 9180

actccttgta taatgcccta gagttggatt agacacactg tgagctcttg ggtggtggtt 9240

ctgaataagg cagtttgtgc agcaaatgca aacacatgca tgggatccag gcaggaacaa 9300

aagcttccct ttacaaagtg ggtgggcatc tggagggagc cctcagaggt gggcagtggt 9360

cgtccttgct gactgcacat tagccagagg cgtgaccata attggtcttg cagggaaaga 9420

gcaccactga ggtcataggt tatgaaaatg tttgtcatcc tccagtgagc aagtccatct 9480

gcttgcttgt gggtgtcaac tccatggtgg atacactctg ggagatgaca agatgcacac 9540

aaacctcctc tcactaatta tccactacca cacactcaag accaaccttt gctccagaaa 9600

ggaatacgtg tctgtggaaa tagacagagc ttaagtattt tgtaacctgg tgaacatact 9660

gtgcaaaacc aaacgtttca ggaagattag aatacaactc tgagtaatgc atgggttttt 9720

gttcatgaaa actctctctc cctaaatcct tgatggatat aacatgacta cataaattgg 9780

gct 9783

<210> 5

<211> 15885

<212> DNA

<213> artificial sequence

<400> 5

aatacaactc tgagtaatgc atgggttttt gttcatgaaa actctctctc cctaaatcct 60

tgatggatat aacatgacta cataaattgg gctgatcatt tttatgctat aaaattaata 120

gatgacactg cactccagcc tgcacaacca agcaagtctt catctgtaaa atctaaaaaa 180

gaaaaattag taggtactga cttcgaaatt tttgataata atattttcac cacccaaatt 240

taatcacacc cacatgttac ctgcatcttc actgaaaagt tcccagtcac gatgagttcc 300

ttcaatgctc catgtgttca aatctggaca tcaagagagt ccagagaata aaacacaatg 360

acggcagtga aactgatata tattcagcac ctcttaactc aggaggactc catacaccct 420

ggcacacagc tgcttttcta aatggctcac aatgactcca gctcactcac agagctcaga 480

cagaaacctc ccttcagggt gggagctggg tggcaggggg cactcagtac ccgcagaggt 540

gaaaatgagc tttcagatgg aacttccctg tcacctcaac atggaattta ttgtttcatt 600

tcattacctc tctttccata atggttcatt tcttttggcc tgttcattac tgatattttt 660

cagagcaatc tcacttgaat ctttactctt ttgcattttg tctccttgac aatgttggga 720

agttttacct ccagcatcat aacatgatct agtgatctga cacattgtgc aaacaatacc 780

tacaaattca gaacctcttt gtttttcttt ccacaaaata taattctttc tgttctgtgt 840

atgagcatgt cttagcaacc ctgtacacac ccacatagat gtctacaagc ctatgaattg 900

ttctctgtaa ataaaaattt atctcaaatt ccttcaatgt tcataattct gagagtgagg 960

aaggtccttc tcaatctgtt caaacaaaat gcccagagac catccagtag gtaaggagtt 1020

cacctggctc tggtgtgggg tctgtctctt tccctctgtt gtcccacagg tcagcccagt 1080

tgttcacgtc ctaacaagaa agcccaggtt tgtcctgatt ttaaaacaca tcaaacttct 1140

gatgactctc ctgttaccca catccatgga gatagattat ttattatata attcagcaaa 1200

ctaatgtcaa atgcccaagt tgcaataccg cacatcctag ggtatgttca tgcaattcaa 1260

tggaggagaa aatctttcag agacagatgg atctgaaatg ataaatatgt gggtaaggac 1320

tctgggcttg agtatcattg tccagccatg tttcacaagt gtgtcctgtc agggaaggat 1380

cagagttcct tgtgctgtca gagggaaggg gtcacagagt tcctctctgg ttcccaggaa 1440

aggtaatcgc actaatcttc atgatcttca tgagactatc ctccagtgct gacctgttac 1500

ggagtttttg tctgaagttc tcactgcaat ccccaatcta catattttca atcagaagtg 1560

tttagaggcc aggacacatc ttcaaggtca cacattgaga aggatggaga tatgtcccac 1620

taccttctcc tacgatctca gacagaatcc caaatttcaa aaggacacag aaggacagct 1680

ctcaggtgct tttaaaaaat gacccacttc cagggacagt gagcttccct gtaaccatgg 1740

tggatgttct gaactacaat aaacattgga tggatccagt attgtttgaa gtcactgtca 1800

ttattacatt cagctgttgt ttcaatgtgt ctgaaagggt aaatgactat ttagatggcc 1860

tgggtgtgtg gttggtttta tatgaatctt taagggttga acagtactga ccctattcca 1920

aaatctgtcc ttgatccagg atcacactca tctctcagac cagctccttc agcacatctc 1980

tttacctgga agaagaggac tctgggcttg gagaggggag gccccaagaa gagaactgag 2040

ttctcaaagg gcacagccag cattctcctc ccagggtgag ctcaaaagac tggcgcctct 2100

ctcatccctt ttcactgctc cgtacaaacg caccaccccc atgcaaatcc tcacttaggc 2160

gcccacagga agccaccaca catttcctta aattcaggtc caactcataa gggaaatgct 2220

ttctgagagt catggatctc atgtgcaaga aaatgaagca cctgtggttc ttcctcctgc 2280

tggtggcggc tcccagatgt gagtgtttct aggatgcaga catggagata tgggaggctg 2340

cctctgatcc cagggctcac tgtgggtttt tctgttcaca ggggtcctgt cccagctgca 2400

gctgcaggag tcgggcccag gactggtgaa gccttcggag accctgtccc tcacctgcac 2460

tgtctctggt ggctccatca gcagtagtag ttactactgg ggctggatcc gccagccccc 2520

agggaagggg ctggagtgga ttgggagtat ctattatagt gggagcacct actacaaccc 2580

gtccctcaag agtcgagtca ccatatccgt agacacgtcc aagaaccagt tctccctgaa 2640

gctgagctct gtgaccgccg cagacacggc tgtgtattac tgtgcgagac acacagtgag 2700

gggaggtgag tgtgagccca gacaaaaacc tccctgcagg gaggctgagg gggcgggcgc 2760

aggtgcagct cagggccagc agggggcgtg cggagctcac ggaatacaag gccgggtcag 2820

gagcaggtgc agggtgagcg gggcttgctc atcttctcag agatcatcat ctccctcctc 2880

gccagcacct cagctttccg tagaggtcct ctttctttat tgtgtgtggt tctacttcct 2940

cacatccttg tgccaggaaa gaaaggagta aggcaaattt tcctgttaca attgaagttt 3000

caccaattac taagaacttt cctgcaagta cctgcacagc ccattatacc ttatttatat 3060

atgtatatat tctaatgctt ctcaccatct cttgatttgt gtcatcaatt taattgtgcc 3120

ctttttgaaa ttcatatgct gaaactttaa atccaatgga tctatatcgg aattttaatg 3180

gtataattaa tgttaaatgt ggtcataaat gagaccctaa tgcaatagag ctgttgtctt 3240

tataagaaga ggaagagaca ccagatacct ctcacttctc acatgcactc agagaagagg 3300

ccacgtggag acatagtgca ctagaaggtg ggcctctgca agccaggaag aagccgcacc 3360

aagaaccaac cctgccagca ccttgatctt ctacattcag actgcagaat tgtaagaaaa 3420

tcaatatttg ttgtttaagc cacccactcc ttttgtcttc ttacgaagac ccagacaggc 3480

taataccaca caactctgtt agctccatct cctggaggga gaagcagccc cctgaagctg 3540

ggcacatcgc tcagattttc acatgaatta ggcaaaaaca gtagctctca tataaaaact 3600

gtcacgtccc tgttgggaca aggtctttta aacaagccct ggggctttgt cacaaatgtt 3660

gcattttatc ctttattagg acttaattaa ttgacaatga gtaccagctg gatggaaact 3720

gaccactgac catcttctgc tgtctcctta ttatatcaca gaaaaccaca gcaacattac 3780

tctatgtctt caactttcta aatttgtact gaatctattg ctaaatgagg agctacatgg 3840

ggtctgagtt ttgttacctt cttcccagtc ttccccaatt accaagcata gaagatactt 3900

tcagtgaaat ttagctgtca atgcccccaa caccacatca tgttttaagg tccaaggact 3960

ttctttgggg ggctattgaa aaacactttt gaatggaaaa tcctaaagca tacaacagct 4020

gaaagaatgg cccctgtgca catgaaggct gaaggggtgg atgatagggt acgttcctcc 4080

aaggtgttcc tgggcatgtg atggttggat acctcatgca tacgaaaaca aggactgaac 4140

tgagatagaa acaagggcca tatcctatag gaaaacaaga aaaaaagggg catccctgaa 4200

gagagttaat tagacttggt gggtttaaga tgaaattttt tccaaagttt tttaatgttc 4260

taagctaaat atgaatattt tcaataaact gcaaattaaa aggagaatga atttagaagt 4320

ttagacagaa aaaagccatg agggtgtaga aagcaaatcc acaaaaaact gtcacatggc 4380

agaggccaga atggagctga tgcagctaca tcattattct gcagacctag ttgagaccct 4440

ttggtgttta aggcaggtcc agcagtgaag aactccttta gtttttgtct gggaaagttc 4500

taatctaatc ttcatttctg aaaaggattt acatggtaaa gattcgtctt ggcagggttt 4560

tatttatttt tagtcccccc caacccacgt caatttgagt aaagtcatgt gttacgtaac 4620

aatgctgcag tcaacagtag accacatata tgtttgtggc cacataatgt tgtaatacca 4680

tattttcact aaactttttc catgttttga tgtgtttaga tagagagata cttaccatgg 4740

tgtttccatt tcctgcagtg ttcagtgcag taacacgctg tacaggttag ttgtctggga 4800

accctgcgct aatccacata ccctgtgtgt agtagggtat tccttgtagg tttgtgaaaa 4860

ttcacccttg atgcttgcac aatgatgaaa ttgcccaggg acacatttct caaaaagaac 4920

ctctgtcaat aaaccacaca ccattgaata caatccctgt gccttctgaa ctgaaaacct 4980

tctgctgaga aatctgttcc ttttctttct gttttctaaa gcctctttta tttccagcaa 5040

tgtggtttta aactgtgtca catcctgcat ttgcaacagc aggcatgact ctagaggacc 5100

atatgtgaag ttccacaggc aggcgcagaa gacaagtaat tcgcgatttc actgtagttt 5160

gtttaaatgg tagactcaca gaagtagagt ggagtgttgg ttaccagggg ctggaggggt 5220

ggactggaaa aggcgagatt ttggtcaaag ggtgaaatgt tccagttaga cagaaggaga 5280

aagttacagt gttctaatgc acaagatgga aaatatggct aataatgcat tatatatttc 5340

aaaatggcta aaaatgcaaa tgttaaaaat tttctcacta agaaatgata cagcctgggc 5400

gcggtggctc acacctgtaa tcccggcact ttgggaggct gaggcgggcg aatcacaagg 5460

tttggagttc aagaccagcc tggccagatg gcaaaacccc gtctccacta aaaacacaaa 5520

aaatagccag gtgtggtggc acgcggttgt aatgccagct actcgggagg ctgatgcagg 5580

agaattgctt gaacccggga gacagaggtt gcagtgagct gagatcatgc cactgcactc 5640

cagcctgggt gacagtgaga ctccaactca aaaaaaaaaa aaagaatgaa atgatagctg 5700

tgtgaggtga tggatatgtc aaatagcttg attaacaatt ccataatata tacatgtatc 5760

atagcatgtg tgaggtgacc tgtatgaagg tgaagtgacc tgggggctgt gtaagattac 5820

ctgggggtat gtgtgaggtg aacacggcac atatgaggtg accagagaca catgtgaggg 5880

gacatgggag atatgtcggg taagccaggg ggatttgtga ggtgacacag agcaagtgtg 5940

aggtgacgtg gatgcatgta aggtgatttg gggcacatgt gaggtgacgt gggggatatg 6000

tgtgagctga cctcgggtga gtgtgaggtg atccaggatg gatgtgaggt gacctggggg 6060

tgtgtgtgag gtgacttggg cacatgtcag gtgacatcgg ggattggtgg tgatgtgggt 6120

gtgtgtgagg tgacctaagg aggtatgagg tcacctgggg catctgtggg gacctggaaa 6180

tgtgtgaggt gggtacaggt gaagtcacct agggcacatg tgaggtgacc tgtggaatgt 6240

gtatggtgac ctgggaccgt ttgaggtgac atggggaatg cgtgaggtga cctggggcat 6300

gtgtgaggtg acaaagggca tgtgtgcagt gacctgggtg catgtgaggt gacctggtgc 6360

atgtgttagg ttacctgggg gatgtgtgag gtgaacaggg gcaggtgtga agtgcctgag 6420

gaatgtgtga ggtgaactgg tgcatgtatg tggtgacctg ggggatttac gaggtgacct 6480

gcgggatgtg tgaggtgacc ttgggtgagc gtgaggtgac ctgacgtatg tatgaggtga 6540

ccagggtgtg tctgaggtga tgtggggtat atgtgcggtg acctgggaaa tgtttgaggt 6600

gacctggtgg atgtgtgagg tgacctgcag gatgtgtaag gtgacactgg ggatgtgtga 6660

ggtgactcag ggcacatgtg aggtgaaatt ggggatgcgt gaggtgattc agggcatatg 6720

tgaggtgacc ttggggatgt gtgaggtcac cttgggtgag tgtgtggtca tctgggatat 6780

ttcccaactt cgtaaggaaa gcctgcaaca acacatggca ttagaagctt cacactctag 6840

caccattaat gcgtacatcc tcaaggaact ctaaatgttt tgaggttagc ttaaatgcca 6900

tggagcggca ccttccccag gcacactcat atatgcatcc tgggtctccc tgccaagcta 6960

tatctcctca aatttacagt ttatgtttgc tctgtgacat caactctgat atgttcaagt 7020

gtgttttttt ttctttattt gtagttgttc aggcttgttg tttcactctc attactctgg 7080

gctcagtcct ctcctcaggt gtcccacttc agagctcgct gtgaaatagg agacatgcaa 7140

atagggcccc ccctttcctg ataaaaagca gcccagtcct gaccctgcag ccctgggaga 7200

gaagcaccag ccctgggatt ctcaggtgtt tccactttgt catcagcaac aaacaaatta 7260

ccatggaatt tgggctgagc tgggtttttc ttgctgctat tttaaaaggt gattcatgaa 7320

gaactaagga tattgagtga gtggacatga gtgagagaaa cagtggattt gtgtggcagt 7380

ttctgaccag ggtgtctctg tgtttgcagg tgtccagtgt gaggtgcagc tggtggagtc 7440

tgggggaggc ttggtacagc ctgggggatc cctgagactc tcctgtgcag cctctggatt 7500

caccttcagt aacagtgaca tgaactgggt ccatcaggct ccaggaaagg ggctggagtg 7560

ggtatcgggt gttagttgga atggcagtag gacgcactat gcagactctg tgaagggccg 7620

attcatcatc tccagagaca attccaggaa caccctgtat ctgcaaacga atagcctgag 7680

ggccgaggac acggctgtgt attactgtgt gagaaacact gtgagaggtc ggaagtgtga 7740

gcccagacac aaacctcctg caggaacgtt gggggaaatc agctgcaggg ggcgctcagg 7800

acccactcat cagagtcaac cccagagcag gtgcacatgg aggctggggt ttgtttcctg 7860

tcaggatttg ggacttcctc tgcttctgac agtttctcta gggaaactct ttaattttag 7920

atttctgtgc ccaccaatgt catctctaca tttttttaat cattgtatat gaggactcgt 7980

tctcacatgc acaatatgta tattgccacc tatgggaatg aaaggtcctc aaccatggtc 8040

accagcatca gagtcgtgag gaagctcagg ggtgcctggt gagtcttctc cagtcagact 8100

caggacagta acctcaaggg gattcccttg tgagaactca cacattttca tgagaacagc 8160

accaggagtc agttctaaac cattcatgaa agacccactc catgacccag tcacctccca 8220

ccaggtcacc ccttcacaac tggggattat aatacaacat gagatttggg gcaggacaca 8280

aatccaaacc atatcagata cacattgtga aatacgcatg gtggtcaggt aatttgtatt 8340

tctatcacct cacagcgcta ccattttatt atttttttta atttactgca tgagtgagtg 8400

tctgatgaga acacctaaga tctacccttt cagcaacaat catttttaca atacagtatt 8460

aactatagga ccattgctgt acgttagatc tccagaactc atccaacctg cacaactgaa 8520

actctgtaca atttaacaca catcacccaa tttccctcac ctcccaggtc ctgggaccca 8580

ctattctact ctctgctttc aagagcttga atattttaga tcccacatgt aaatgagatc 8640

atgcagcatt tgtctttctg catctggctt attcaactca gcatcatgtc ctccaggccc 8700

atccgtgttg ttgcaaatgt cagaatttcc ctcttttcaa agccaaacaa aatacagatg 8760

tatgtataca cattttcttt atacattcat ccatttacag tcattaaatt attttacaaa 8820

tctacactat tattaataat cttgcaatga acatgtcttt ggcaaagtaa ttttatttcc 8880

tttgcatata taacaagaag tgggatcacc agattatatg atagctttat ttttaactta 8940

tcaagtaacc aatcctacca caggatattt ccctttcccc cacattcttg ccaacatttg 9000

tcatctgtta catttctgat aatagccata ttaactagtg tgagttgata ttgcattgtg 9060

cttctcattt gaattcctct gataattagg aatgttgaga actttttcgt tttctgtttg 9120

ccatgcatgt atcttctgaa aaaaattatc caggtttttg ccctttttta tcagttcatt 9180

tgttatttgc tgttgaggtg tatgggttat ttatacattt ggacagaact tcttgtcaga 9240

tccataattg cacatagttt ttcctgtgct ttgttattaa attcaaagaa atcagttccg 9300

aattaatggc aggaattttt tttgctccat gtcatttatg agtttatggc ttcaggtatt 9360

atgtccattt ttagttgatt tttttttttt ttgggagacg gagtcttgct ctgtagccca 9420

ggctggagtg cagtggcgcg atctcggctc actgcaagct ccgcctcccg agttcacgcc 9480

attctcctgc ctcagcctcc gagtagctgg gactacaggc gctggccacc gcgcctagct 9540

aatttttttt tgtattttta gtagagacgg ggtttcactg tggtctccat ctcctgacct 9600

cgtgatccgc ccgcctcggc ctcccaaagt gctgggatta caggcgtgag ccaccgcgcg 9660

cggcctatta gttgattttt ttatatggag ttagagaagg cctaatttta tttcttttgc 9720

atatgaatgc cagttttaca ccattattga aaagactgtc ctttctctac tgtgtgctct 9780

tggcacacaa aatcaggaga gacataatga caaaaaaaat atgagatcaa tagtcctgat 9840

gaacatagac ctgaaagtcc tcaacaaaat accatcaaat tgaatccaga agcactttaa 9900

aatgtgatac atcatggtca ggtgggcttt acccctggga tgcaaggctc gttcaatatc 9960

cacagtaact ctgattcaca atgtaaacag aataaaagca aaaagcatat gattatggac 10020

tacgtaaatt ggactgatcg tttttatgct gttaaattaa taggtgagtc tgcactccag 10080

cctgggcaac agaataatct tgtctgtaaa atacaaaaga aagataaatt aatagatact 10140

gactttgaca tttcggataa taatattttc ataaaccgaa tttaattata cccacattgt 10200

tacctacacc ttcactgaaa agttcctagt tatgttgagt tccatcaaca ctccacatgt 10260

tcaaatctgg acatccaaga gagtctagag aataaaacgc aatgagggca gtgaaacttg 10320

cgtatattca gcacctctta actcaggagg actcaataca ccctggaaca ctctgctttt 10380

ctgaatggct cacaatgact ccagctcact ctccaacctc ctcaaacatc tggcctctgt 10440

ttgccctaag ttcacgctct gctcttagtc tatgttctga agtctttgta gaggtgaaaa 10500

tgagctgtca gatggatctt ccttctcact gcaacatgga atttgctatt tcacttaatg 10560

accactcttt ccacaatggt tgatttcttt tggcctgttc attactggtg attttcaagg 10620

gaatctcagt tgaatcttta ctgttttgca ttttgtctcc atgacaatgt tgggaagttt 10680

ttcttctagc agcataacat gatctagtga cctgacacat ttgcagcaaa caatacctac 10740

aaattcagaa gctctttggt tttctttcca cgaaatataa ttcttgctct tctgtgtatg 10800

agcacatcct agcatccctg tacacaccca cgtagatgtc tacacgccga tgaaatattc 10860

cctgtaaata aaaaaagtat ctcagtttct ctcaatgttc ataattctcc tgagggtgag 10920

gaaggtactt ctgggtctgc tcaaacaaat ggcccagaga ccacctggta ggtaggtaag 10980

gagctcacct cgctctggat attgagtctg tctctttccc tctgtcgtct catagaaggc 11040

cagcccactt gttcagctcc taagaagaga gcccaggttt atccagatta tacaacacaa 11100

ccagcttctg atgactctcc tgttacaaca tccatggaga tattttgtgt attatataat 11160

tcaccaaact aatgtgaaat gcccaagttg caatactgca caccctaggg tatgttcttg 11220

caattcagcg gaggagaaat tctttcagag acagatggat ctgaattggt aaatatgtgg 11280

gtacgaattc tgggcttgag tgtcattgtc cagccatgtt tcacaggtgt gacctgtcag 11340

ggaagaacca gagttccttg ttctctcaga gggtagagct cacagaggtc ctctctggtt 11400

cccaggaaag gtaatttcac taatcttggt gatgagacta tcctccagtg ctgatgtact 11460

atagagtttt catctgaagc tgtcactgct atccccaatg tacatctttt cacacagaaa 11520

tgtttagagg tcaggccata ttctcagggt tacacattga gaaggatgga gatatattct 11580

actaccttct cctgagatct cacacacaat ctcaaatttc aaaaggtctc agaagggcag 11640

ctctcaggta ctatttaaaa ataacccact tcctgggaca ggtagcatcc ttctaaccat 11700

gatggatgtt ctgaactaca gtacacattg catggatcca ggtttgtctc aattcactgt 11760

gattattaca ctcagcagct gtttcaatat gtctgaaggg gtaaatgaca atttaggtga 11820

cctgggtgta tggttggtgt tatatgaatc tttaaatgta gaacagtatt aactgtattc 11880

caaaatctgt ctttgatcca tgatcacact tgtctcccag accagctcct tcagcacatt 11940

tcctacctgg aagaagagga ctctgggttt ggtgagggga ggccacagga agagaactga 12000

gttctcagag ggcacagcca gcatacacct cccagggtga gcccaaaaga ctggggcctc 12060

cctcatccct ttttacctat ccatacaaag gcaccaccca catgcaaatc ctcacttagg 12120

cacccacagg aaatgactac acatttcctt aaattcaggg tccagctcac atgggaagtg 12180

ctttctgaga gtcatggacc tcctgcacaa gaacatgaaa cacctgtggt tcttcctcct 12240

cctggtggca gctcccagat gtgagtgtct caggaatgcg gatatgaaga tatgagatgc 12300

tgcctctgat cccagggctc actgtgggtt tctctgttca caggggtcct gtcccaggtg 12360

cagctacagc agtggggcgc aggactgttg aagccttcgg agaccctgtc cctcacctgc 12420

gctgtctatg gtgggtcctt cagtggttac tactggagct ggatccgcca gcccccaggg 12480

aaggggctgg agtggattgg ggaaatcaat catagtggaa gcaccaacta caacccgtcc 12540

ctcaagagtc gagtcaccat atcagtagac acgtccaaga accagttctc cctgaagctg 12600

agctctgtga ccgccgcgga cacggctgtg tattactgtg cgagaggcac agtgagggga 12660

ggtgagtgtg agcccagaca aaaacctccc tgcaggtagg cagagggggc gggcgcaggt 12720

actgctcaag accagcaggt ggcgcgcggc gcccacagat cccgaggccg ggtccggagc 12780

aggtgcaagg agggcggggc ttcctcaaca gctcagtggt ctgtctcctc gccagcacct 12840

cagatgtccc caggactctc tttctttatt atctgtggtt ctgcttcctc acatccttgt 12900

ggcagggaag aaaggaggaa gacaattttt ctgtttactg ttgaggtttc accaattact 12960

agaaactttc ctacaagttc ctgcatgact cattttgcca tatatggatt ctcaccatct 13020

cttgatttgt ttcatcaatc gaattgtgcc ctatttgaaa ttaacttact gaaaccttaa 13080

atccaatgga tctatactgg aattttaatg atgtaattga ggttaaatgt ggtcaaagtg 13140

tgagacccta atgcaataaa ccgttgtctt tataagaaga ggaagagaca ccagagacct 13200

ctcacttttc acgtgcacac agagaagagg ccatgtggag acgtagtgca ctagaaggtg 13260

gccctgtgca agccaggaag aagccatact aagaaccaat tttgccagct ccttgatctt 13320

cgacattcag actgtagaat tgtaagaaaa tcaatatttg ttgtttaagc cacccaatcc 13380

tgttgtcttc ttacgaagac ccaaactgac taataccacc taactctgtt agctctgtct 13440

cctggaggga gaagcagccc cctgaggctg ggcactgcat ctctcagatt tccacgtgaa 13500

gtaggcaaaa atagtagttc tcatataaaa atgtgtcatg gctctgttgg ccatttttga 13560

gcatggtctc tgaaaccagc cctggtgtgt gtgtgtaaca aatgtccctt atcttttatt 13620

tggacataac aaatagacga taggtaccag ctggatggag attggccact gatcatcttc 13680

tgttctcctt agtatgtcac agaaaaccac accaacatca ccagcatcac tgtttttctt 13740

ctaccacctc aaacggacta cagaaatgat ccctgcagta tggtctattt cttcggcttt 13800

cgaaatttgc actgaatctc ttcctaaatg gggagctaca tggggtctga gttttgttcc 13860

tttcttccca gtcttcccca agtaccaagg acagaataga cttaaaattt ggccatcaat 13920

gcccccaaac accacatcac tttctaaaat ccacatcctg catccatcct tctctggaca 13980

cccctcatca ggctacccag gaatggccag aatctggtat cagcttatgg gctgaggcca 14040

cgagttatac acatgtgtga tttcagtcac acacactcta cttcaggacc acacctgtgt 14100

tctgaggagc tcaggcacct gctgatctca gtaattctct aataaatcac acacctctta 14160

ttaataaagg tccagatggc cccatcagct gcagagcagt ggagtaaagc tcatgggtgg 14220

gtccatcagg cagaagtcag acaatggaag ggatggctgg tcacttccat tttcactgat 14280

gttcgcaatg aattttaatg gaataaaaca atattaacta caaagagaca gaaaagaaga 14340

ctggctcgtc aagatattta aggacaagga actttatttg gggggaaagt gaaagacact 14400

tctaaatgga aatccctaaa gcacatacag cagctgacag agtggccact gtgcacatga 14460

gggctgagga gatggatggt agagtccatt cctgcaacat gtctctgggt gtgtgatggt 14520

tagactcctc atgcatatga atataagagc tggactcatg gagaaaaaaa gggccatatc 14580

ccataggaaa ggaagaaaaa aaaacagatg ggcacccctg gagagagctc attagatttg 14640

gtgtgtttaa gatgaaaatt tcttccaaaa attgtaatgt tctaagctaa atatgaatct 14700

cttcaataaa ctgagaatag gagaatggag ctaggacttg agagaggaaa caaatcttga 14760

gagagcagaa agcaaatcca caaaaactgt cacatgacag aggtcagaat ggagctgatg 14820

tagctacttc actattctgc agacttattg accatgtgga gaaggggctt gaacaaatgg 14880

agacgttctc caaccttctg aatcagcctc cttcttatgc atgagtagaa atcatagttc 14940

tgggggtgac cttcccaatt ttcctgtcca tacctcttcc cccaggggta gagtgtcttc 15000

ccaaccacaa tggttccttc cacctcagcc tcccaaagtg ctggaattat aggcgtgagg 15060

caccgaattc aggtccaact cataagggaa atgctttctg agagtcatgg acctcctgtg 15120

caagaacatg aagcacctgt ggtttttcct cctgctggtg gcagctccca gatgtgagtg 15180

tctcagggat ccagacgtga agatatggga agtgcctctg atcccagggc tcaccgtggg 15240

tttttctgtt cacaggggtc ctgtcccagg tgcagctgca ggagtcgggc ccaggactgg 15300

tgaagccttc ggagaccctg tccctcacct gcactgtctc tggttactcc atcagcagtg 15360

gttactactg gggctggatc cggcagcccc cagggaaggg gctggagtgg attgggagta 15420

tctatcatag tgggagcacc tactacaacc cgtccctcaa gagtcgagtc accatatcag 15480

tagacacgtc caagaaccag ttctccctga agctgagctc tgtgaccgcc gcagacacgg 15540

ccgtgtatta ctgtgcgaga gacacagtga ggggaggtga gtgtgagccc agacacaaac 15600

ctccctgcag ggaggctgag gggaccggcg caggtgcagc tcacggccag cagggggcgc 15660

gcggagctca cggaatacaa ggccgggtca ggagcaggtg cagggtgagc ggggcttgct 15720

catcttctca gagatcatca tctccctcct cgccagcacc tcagctttcc gtagaggtcc 15780

tctttcttta ttgtctgtgg ttctacttcc tcacatcctt gtgccaggaa agaaaggagt 15840

aaggcaaatt ttcctgtaat cctgagtaag gtggccactt tgaca 15885

<210> 6

<211> 12329

<212> DNA

<213> artificial sequence

<400> 6

tgtggttcta cttcctcaca tccttgtgcc aggaaagaaa ggagtaaggc aaattttcct 60

gtaatcctga gtaaggtggc cactttgaca gtcttctcat gctgcctctg ccaccttctc 120

tgccagaaga taccatttca actttaacac agcatgatcg aaacatacaa ccaaacttct 180

ccccgatctg cggccactgg actgcccatc agcatgaaaa tttttatgta tttacttact 240

gtttttctta tcacccagat gattgggtca gcactttttg ctgtgtatct tcatagaagg 300

ttggacaagg taagatgaac cacaagcctt tattaactaa atttggggtc cttactaatt 360

cataggttgg ttctacccaa atgatggatg atggtagaaa ccaaatagaa gaatggtctt 420

gtggcataat gtttgttgcc tagtcaatga agtctcatat tcttgtctct ggttaggatc 480

ttgggatctg gagtcagact gcctgggttc aaatcttggc tctgcccata ccatctctgt 540

tatcctgggg caagtgcctc agtttccaca tctgagaaat ggggatggta ttggtgtcca 600

tttcatagat taagtgagtt tagccttgta aaaagcttag gagggggtct gatacatagt 660

aagcactatg tacgcactag ctataattat ttgctaaagt tctgctttaa aagtaagcta 720

tttttttatg gagacagctt ttttctttta aatttccagc taggcaagaa gagcgtcaat 780

ttgatctaaa atttcataat gcttcagatt aacatagaca tggataagtc ccagaatttg 840

cagtctttta gtaaaagtag cattttctgt gtaattcttc acaagcactg attgtagttg 900

caggatgctc agtctccctc tgagatgttt tacattttta aatggttaga cttgcaggaa 960

caaaagagca gagtaactta gtaggctgtt ttgcattctt aggaaaagaa aaccatcagg 1020

acttattttg ttttcatgta ttttttcact tccactgagg agtataattg gctggtgttg 1080

acaaaatacc aatcatagat gtaaaggaga aagttgatta gttttctggc tgttcctaaa 1140

attctggatg caggaactgt ggctagaaag catctggatg attgcacttt atcagggata 1200

cttgagtgtc ctctcttagg atctggacct agaattaatg tcatgagatt tttctaacag 1260

gataaggtga ggtagtgagg gctgaagtca tccactgggt tatccaaata ttaggtttca 1320

ctgctgacaa aagagggggc ttctggtctg gttggttatt tgtgtttggc ctgatgtgct 1380

ctgtcaatca aatgtatgga cataggccta gcttctaaag gggcaatagt gacctcagtg 1440

gactgatatt taccgtacta tttacatgtg ctcttaatta cagcagaagc tgccagctaa 1500

ctgaatcttg ttttgaatct aaaaaatcta ctcttaaagc aagaaaatgg tataaaatta 1560

gttgataatg caagtgaatt ctgtacattt aattattcta agacattgga aaataaaata 1620

tcttgttact ttgaggataa aagatgattt ctttaaaaat gcaaatgttt tctacaaata 1680

ctaaagttaa aagggagaga gatgtaatta gaactcgtta actgacacat tgcaaattaa 1740

cttcttttta taaagcactg catcacaaac actaaaatga agtgggcaaa ttagctctgc 1800

agaaaactat tttctaggct gatgtttata atgaccaatc attactgaag caatgagaaa 1860

tgtgacaatt acagaatatt gctgctatag tatgttgaaa aaatatgcat tttgtagtga 1920

acatttagta gaatagctct gatttctacc tggagtttct gataacatga catcttaatt 1980

gctgtctttt atagattttt aaactgcaaa tacaaaatag caatcagcca atataataac 2040

ttattattct ccatttatgc ctgaaagtcc tcctcttgtt gatgccgtgg aaatgaatgt 2100

agaggcagat atcattagct gtattctcct tccgaatgac atttatcata tccttgttat 2160

tccaaaatag atagaagatg aaaggaatct tcatgaagat tttgtattca tgaaaacgat 2220

acagagatgc aacacaggag aaagatcctt atccttactg aactgtgagg agattaaaag 2280

ccagtttgaa ggctttgtga aggtaagcag cttaattact ggtaaaagtg tcattgaaat 2340

attttactac atttgctaga tcgggaaact gacaatgcca atgtttaaag attggttata 2400

gacacagaca cacagacaca cacacacata tatatgcatg cagatataca cacatacatg 2460

ggtgtgtgtg tgggggttaa aaaaaaaaaa cacaaagaca ctctctgggg aaaatacacc 2520

cttaggggca cagtcacaca tatttgtcag cttacatatg cagctaccac taggcaaaat 2580

gatgaagtcc accaagcttg gtttttgcat tgctgtgtct ccccatccaa accttgatgc 2640

tctcgcactg gggacccaga gtctgatccc catttcccag ggaagcaata gccgtcaaca 2700

gctgccgtgg cagcaggcca caagtgaagg gacacctgaa gactggtaac agtctctggt 2760

gcttctctga tgatggaatt ttaggtgtcc tgacagtgag atctttccct tttactgggg 2820

agagaggtgc agggaataag taatagacat tctcagtgtc gctcaaacca gactccatat 2880

aatatcactt gctcatgaag cccgcccact ctatggccgg tcatgaccag aggcacagag 2940

ggttcaaagc cttttagccc accaggctgg tagctagcat gaagtcactg cagtgactgt 3000

ggcttataac agatacctaa aacaagaatt tttagaacct ttacattaat tccatcatca 3060

cagacatagg gtctaggggc tctttctcct gaggcagaac atcaagagtt ctttctgcct 3120

atgtcccttt cagaacactg agtcaaatac ccttgggcct cggctcactt aggggtcatt 3180

tctaggaggc agcactccac attgaggaca gttctgggcc aggtgggtgg gtatctgggt 3240

aaaccaacag gaattagttc tcacatatag atgatgtgta atttaatgca ggcgtaaaag 3300

ggttaagatc ttatttctga tcttatttct gccctcctgt actgtcaccg aggtgccatt 3360

taattcatta gtgaagactc taacagctta ttcctgagtc acctacggag aacagaatgt 3420

ggctcaaatc cgctgcttgc tttcaggttc tttacactaa tctaggcttt agatgaaact 3480

cctaaaccct ttctttgcaa gactggccag ctaggaaaat gatttgagtt tcttcggttc 3540

ttcgaggatt tgggccagta ttacagagta ttggaagatg ttaccagttt aaatgtgaat 3600

aaaggcactt tcaaaacaat ggctaataat ccaaataaca gactgaatgt gcttggctat 3660

gtgactttgg gtaaataact tcacctttct gggcctcagt tttgtcatct ataacatgag 3720

aagacagatt atctgtaagg gcactatcag ctctgacatt ctacaattat gtgataagcc 3780

ttcagttccc tccaatggca gtgagagtgg cttgtcagtc cccctcgttt cttacggaga 3840

cttttacggt tgaattgtca attcctcacg tcattatttc aggttggcta tgtatgtaaa 3900

gctcccaaaa tcagctaccg aggataggag taaagaaaac agtcagtttg gcctccctgc 3960

ttatgcttgt atgaaaaaag tgacagctcc aaagtttcat attcttaaaa ggcagatctt 4020

ctcaggcatg tcagccaggg ccccagggat ctcctcctta catgcaacta aggaggctcc 4080

ttgtctctac tgcagcaggt gtggaaccct agtcaacacc acctatacct aggattacgt 4140

acaatgagta gatacaaagt cctccagcta cccaatcctc ccccaatgac ggatcccctt 4200

tccaatacgc tttcccccaa atttctcacc ctaaaacaaa attcgagact ttgaaaaaac 4260

tcaataggac aattatagaa tagctccaga ttagattcat attttcttag ctaatgttag 4320

taggctttct ttccgggcca cagtctggct gcacctaagc aacctcaagt ttgaatttgg 4380

agtctttgaa tcaggtcttg atggggtctt agaagtcatc agatccaatt ctcaatccac 4440

aacttcagtc ttctctccac ctcctgacta agtggtcatc caatctctgt ttgaacatct 4500

ctagtgacaa ggaactcatt atctctggag gcaggtagca ctaatctgtc attttggggg 4560

aaagatggta ttcagggctc aagtgagggt aagcagaggt attattttga atagtataat 4620

ttcatattaa aacttacaac ccaccacacc tctgctagat gttcagttcc atgattattt 4680

gcccaccaat gcctgcgatg cctttgagag agccaaagca tttctatttc aagttaaagg 4740

gcaacctgtc catacctgcc acatggaact cccactaaga gagaaataac ccattctgga 4800

ttttctgaaa gtccacttta aaaagtattt cagttgaggt ggggagtgaa gcaagaaaaa 4860

aaaaaggctc tggggagtgt ggttgggcga aagttcacgg aaaggctagg ctgggctcat 4920

gaaacacgag ctttgctgac ttcatgtttt catcttggcc aggcctcaac accaatgcaa 4980

caacttagcc taaaagtatc tcaaccttga tcaccacact ctactttttg aaaagacact 5040

aaatagtcat ttgtttactt gtgatctcac aaacattttc ctgtcaccac atcttcatag 5100

tgccgcgctt cagctcaaat ggaaagttga agctctgggg cccatgtgag tgttctgagg 5160

ctcaggttcc cctggaggct ctatgaacta cgcccttaaa tctggcaact gagctgggcc 5220

tacagccagc actcaacagt gacagcacaa attccttctg gaggaggaaa taaaaggaag 5280

ggtcctatag acaactgatt ccaggagtgg gaaggagcac aggactttga ttatcataag 5340

atgtgaaaat actactgtct tcttcccttg tgtgcagagg atagacagat ggaattagct 5400

aagcccagcc tatgaatgcc atctcacagt ttccactctt ggtttaaacc tcagcttctt 5460

tgggtgacct cataatgacc agttaagccc tccaggcctt ttgttcagtc tctttaaaat 5520

ggcagcaaca gcctttatca tcttccaacc tgtgttgatg gaagttcctg ttagcttctt 5580

taaatacctc tagacttcct tcagtttata agtgaaaaga aaccttttaa gaagtgtcgc 5640

acttgccttt gaacatcaac accattggga gatggcctgt gtttccgaaa tgctgattat 5700

tctaagtaaa tacagtgcaa ctatcaataa gagaatctct tcagcccatt gaaagggata 5760

gcaaaattaa aaatgtctga gggtcttttc atagtctggc atttctcccc aaggtcaaac 5820

ttactattat cttttcctac aggatttcag accaaattta ttctaataga tacacaccat 5880

gctttatgtt taataatatt ccatatacca gttcccaggg tagaatcatc tccccattcg 5940

gcattatttg tcaatatctg tcaaagccaa ggaggttgag gtcataggaa gggtcaggat 6000

cacagcctct ggtctggaga gagcactgga atggagataa taaggcctgg attttacttc 6060

cagattctcc cctgggcttt ctgggttgtt ggctcatctg tcagatccat ggactcccaa 6120

ttggcatgat ggaattaatg acaggatctg agtctatatg ataatcctca ccagaaacag 6180

acaacagagt aatgacagat gcaaaacgaa tgataatttt aaaaccccac agcagagccc 6240

ctgtcaaaat gacctcttgc aatgcttctt attttaggat ataatgttaa acaaagagga 6300

gacgaagaaa gaaaacagct ttgaaatgca aaaaggtagg tttgctattt gctaatttct 6360

atgaatgcct aaaaactaaa aggaagcttt aggctgatca tattgaacaa cccagtgttg 6420

ttgcatcagg gaacttttag ccctggaaat aaaacaggaa cacaattgtc aaattgacac 6480

cttctctggt ccctgtgatt tggaaagact ttgtacatat atatttatga aaaaaggatg 6540

tgttccttta atgccgatga taccaaatct gaagaaatcc cattatgttc aataccttaa 6600

tagaagcaac catacagcct gataccacct acagtggaat aagaagacag gaaagtcatc 6660

atttggtaac agtggcattc atcactcatt gataacagtt tttcatgggg cacagtggcc 6720

ggtggagcct ctgggatcaa ggagtgacaa tgtcacagtg ttctattatt tgcccggttc 6780

ttaaagtgag agcatcctga acatctcagg gttggaagag aacttgagag ttctcaaatc 6840

cagcaccatc cccacaacaa aaatctcctt cacaataaca ctgaccgtcc agcctctgat 6900

caaacatgtc gagggatgag gcaccttcca cctcataagg cagcctgatc cgtctttgaa 6960

tggctctaat aataccaaga ttactatact actccagaga agtctttcct cctcaagtca 7020

aactttgttc ctataatctc cactcattgg tcccagttct gctctttgag gccctagtaa 7080

acaaagtata attgctctcc tacccagcag ctgtccagat atggaagaca gcaatcatgg 7140

tggccaagcc ttgactgagc tttttcttct ccaggctaaa gatccctgat gtcttccact 7200

gtttctccta tgaccctttc caggaccttt cttctgccac tcacctcctt ttcttggaca 7260

cactaacgtt ttcctgttct tttagaatgt ggcatcgcaa accaatacaa taatgcgtga 7320

agtgacttca gcagcagatt atgggaaaga cggggtgttg ttagagagaa ttttatatca 7380

caaagttggt gaacatgatg ttatggcttc tgcaaattta atacacacaa aaacatacat 7440

acatacaggg atagagatac tattttctga ggcaaagaga gtactcagac cttgccttaa 7500

ctgttgttct ggatactaaa tggtcatccg acttccatga aggttttatc ttcagaatga 7560

ctgcaagata tgttgagtaa tagtaccacg ctgtctgtta attacagaga aatctgagga 7620

aacagtttat gtagatgctg cctagaagtc ttcagggaaa tgataatatt aaccaaactg 7680

gtcatttagg tcatgcaatt taactcaaca tttatagggc acttacaaag tgcccaatat 7740

caggctcata actggacaaa aagaaacttc cacacagtct ctgcccttag aagattgaca 7800

catctcatta gggagcaggg ctttaacaca agaaataatt aaagacagat acaatagttc 7860

agccagttgc ttgaccaatt cagaaaccat aagaatctta ctaagtgtgc agactttgga 7920

gcccactaaa atccccagtg tatggagttg ttcctaaaag caagattcac ggtatgttta 7980

atgaagacca gtgtttttag cctgtgtcaa tctatgcaaa atggaatcga gtattgatca 8040

actgttagga gaatgagacc gatggaaaca gccaattcaa ttactcagat attagaaacc 8100

aacttttcct tcagtgggag agatgtcaga ccattttatc tttcctttta tataatctat 8160

ttttgcacag tctctattac acagttgtag aactggacca gatagttttg tgggcagttt 8220

ttgcattatt ttagcctgac agtttttggt tccatttcag gtgatcagaa tcctcaaatt 8280

gcggcacatg tcataagtga ggccagcagt aaaacaacat ctggtaagtc acacagcatc 8340

tgagcggtag ccacccaagg ggaaaggctg ggatgccgaa gtcatgttac ctaatggtta 8400

aactcctctt ttcccctggg acccaattta caaacctacc cctacacttc tcctattccc 8460

ttctttgtct tcaaagtgag ttcaaatgca cagatgggac ttagagggac aaaaggaggt 8520

ggaatgcaat ctggatgttc tcattatgtt cttgctcaat ggctgattct aaatgatgaa 8580

ttactgggtg gagggaccat tgttctgaca acatagaaga aatggcatgt agtgacctcc 8640

tgactgggag catccctcct cctaacccat cttcactgtg tggaaatggg cctcatgggg 8700

tatttcctgc catctgtcaa tccctgtatg attaagctca gcctcactga ggccaacctc 8760

agggaaagta aaggtaaaat cattctgtaa agatcaatag gtcccaagac gttacatttt 8820

ccaatgaagt aacaacagac gacatattgt gatcttttca actctgaacg attttatttc 8880

catatacgtt ctgccaccat tctagccttt agatattttt tcccaaatgt gcatcttgcg 8940

ataactggtg ccaaagaata tgtcgtatct gataaatgga tggaaacatg cacgctaaca 9000

taaagtctcc catcaacata aaggcaagag cgtcagagga gtctttgaaa aattctacag 9060

agtgctccgg aatggagttc taagcagtgc atgtgtgtgt gcatatgtgt atgtgtgtga 9120

cagggagaga aagagagatg gacagagaga gaaaaaagac actgcttcat ctctgaagtg 9180

gcttgggctt ctcagtaggc gtaacacatg gacagttatc attatcatgg atcatggtac 9240

caaagtaaga gcactgaata gggagttttt gaacactggg attcaaggac catgaccact 9300

gcttgctggg tgaccttgag caagaccctt tacctatgca gcagttttct acttcaccta 9360

ctttacaggg tggctttgag catcaaatca gctaatgtgg ccgaaagtga tgctgtcgag 9420

tgctgtacaa ccgtaaggtg acactactta gtttacttca ccatggctta gatgtcaaaa 9480

gggtgacata aagcccctca ctaataccag ttagttacac aatatttaat aattttgtca 9540

agtacccctt ctctcttctg gatcagatga caacaacaga gaaatctcct agaagaatag 9600

cttcccactg gtcttttttt gcctgtatct aaacccttga tcttggatat atttcataga 9660

gctcagattc tcccaaaagg cttgtaatgg atatcagtcc tacaatatct tacagtctgc 9720

atcacaatag gtttccaggg gatcagatgg gaagacagta acattccacc cccaccccag 9780

tcccaaacct cttcttccta cctagccatg ctgctaaaat cttgccctac atcccacagc 9840

aagtactaaa attaggtaag gacgtaccaa agtaaactta ctgaactaaa agattgagaa 9900

cctgcccttt ttttctcaat aaaatggttc aaaagggcaa acattctaat gaagcattgt 9960

ttctggagtg gtctggaggg cccggatctg tcaggcattt caggatgcct ccctattagt 10020

aaagggcgag tcttaccagg tgggatcttg tgccctgata gacctaagac tatcgaatag 10080

gaattatttt ttaaaaagct caaggaagca aacacatcag tactttcact tttcctcaac 10140

cctcaccccc atcagtcagt ctagctttct gtgggagctg agatttcaag tcgggtgcac 10200

acactacttt gaacccactc aacatctcag ccgagaaaat ggcacactgt tggtgggtac 10260

tctggcttag ccacaagaat actggtactt tcaagttggt ggcgcccact acaatgggag 10320

atcaaaacat accgtgaaat gagcacacag tttattttca tacttccttg cctaatttta 10380

gtccttgctg ggggaggcag atcaggtttg caacagcatg atcaggtagg aagaaatggg 10440

gtcttttctc tgtgctgagg ctgagctagg tagactgaca actctctgac tttgtaaaat 10500

tcaaggcaag caaggtattc atggtaatat tagcaaaaat ttggtccgag taatttggta 10560

tgtataattt atgatgtcaa attttgaaat catttgtgcc ttcttaagtt caaggcaaat 10620

tggctataag aactctaacg agagaaagaa actcactgtg atctcttact ttatttaatc 10680

ttcacaagtc tctgaaatat gctccaatat gagccccgtg ttgcagatga ggaactgaag 10740

ctcatggaga tttagagact tgcccaagct taaatagagc ctagattgga acatggctct 10800

gtctgactct gaagcccatg gaaggggcct tgagaatcca tccctataca aagccaatat 10860

ccaacattaa actatatttt ttgtcagaat gtgaaccatg ctctgcttca cctcaccaca 10920

aactttccct ttctttgtaa cagtgttaca gtgggctgaa aaaggatact acaccatgag 10980

caacaacttg gtaaccctgg aaaatgggaa acagctgacc gttaaaagac aaggactcta 11040

ttatatctat gcccaagtca ccttctgttc caatcgggaa gcttcgagtc aagctccatt 11100

tatagccagc ctctgcctaa agtcccccgg tagattcgag agaatcttac tcagagctgc 11160

aaatacccac agttccgcca aaccttgcgg gcaacaatcc attcacttgg gaggagtatt 11220

tgaattgcaa ccaggtgctt cggtgtttgt caatgtgact gatccaagcc aagtgagcca 11280

tggcactggc ttcacgtcct ttggcttact caaactctga acagtgtcac cttgcaggct 11340

gtggtggagc tgacgctggg agtcttcata atacagcaca gcggttaagc ccaccccctg 11400

ttaactgcct atttataacc ctaggatcct ccttatggag aactatttat tatacactcc 11460

aaggcatgta gaactgtaat aagtgaatta caggtcacat gaaaccaaaa cgggccctgc 11520

tccataagag cttatatatc tgaagcagca accccactga tgcagacatc cagagagtcc 11580

tatgaaaaga caaggccatt atgcacaggt tgaattctga gtaaacagca gataacttgc 11640

caagttcagt tttgtttctt tgcgtgcagt gtctttccat ggataatgca tttgatttat 11700

cagtgaagat gcagaaggga aatggggagc ctcagctcac attcagttat ggttgactct 11760

gggttcctat ggccttgttg gagggggcca ggctctagaa cgtctaacac agtggagaac 11820

cgaaaccccc cccccccccc cgccaccctc tcggacagtt attcattctc tttcaatctc 11880

tctctctcca tctctctctt tcagtctctc tctctcaacc tctttcttcc aatctctctt 11940

tctcaatctc tctgtttccc tttgtcagtc tcttccctcc cccagtctct cttctcaatc 12000

cccctttcta acacacacac acacacacac acacacacac acacacacac acacacacac 12060

acagagtcag gccgttgcta gtcagttctc ttctttccac cctgtcccta tctctaccac 12120

tatagatgag ggtgaggagt agggagtgca gccctgagcc tgcccactcc tcattacgaa 12180

atgactgtat ttaaaggaaa tctattgtat ctacctgcag tctccattgt ttccagagtg 12240

aacttgtaat tatcttgtta tttatttttt gaataataaa gacctcttaa cattagtcca 12300

tacctcttcc cccaggggta gagtgtctt 12329

<210> 7

<211> 14861

<212> DNA

<213> artificial sequence

<400> 7

ttgtttccag agtgacttgt aattatcttg ttatttattt tttgaataat aaagacctct 60

taacattagt ccatacctct tcccccaggg gtagagtgtc ttcccaacca caatggttcc 120

ttccacctca gcctcccaaa gtgctggaat tataggcgtg aggcaccgca actggcctct 180

tcatttttat tcatatgttc cttcagcagc cactatgtct tcccactgat ttcttcagtt 240

tctgcctttt ccttttgaat aaggctgtta ctcctgaggg aagatgggag gtgggcctgg 300

acagggactt ggtgcattcc tctctcctgt cccagttctt attggtttct ccagtgtctg 360

tagaacagtg gttttggtgg ctttacctct gcagataatt tctcttgcaa tgtagtggtg 420

atggggaggt gtgtctggat gcatttcagc tatagttgct gttttgcttt cccagacagc 480

accatcccaa agggtagagg ctggagcatt ttgtgatgta tccccagtac tgaagaaaaa 540

ggcttcaata gcaggaggaa ttcctcaact gtatacactc tgagaattta aacaataact 600

tctctatcac actcaaattg aaaccatcca atgaatatgt ctactttaat cgtgtgctaa 660

cttaaatggc atttggcagc ctctgtccca gaaaagatta tcatctgctc ctgtttattt 720

ccctgcacgt ccttatctct cttcagattt cagatatatt gtttgtccta taacatcaaa 780

aatttgatgt atatgtgcta atttgcagat cagtaagttt agtagctgtt gtaagaataa 840

taacatattt ttatcgggtg cttacatctc caagctgaga agcacctcta tgtgtaatac 900

taagaaacta gaaatgatac aaatatcaag aagatacata gataaaaagt aatggcatgc 960

taatttactg taataacatc catcatgaga atcaatacat tgttgatgct caacatgttt 1020

gcatcataag tagttacgtg cgagaagcca cacaaataaa acacatacta tataattcct 1080

gtataataaa ttcttgaaac tcaaaactaa ggtattaaat gtgaaggact gactcagaat 1140

atggtggggg aagaaaataa ttgggaagga ggaattgtag agcaacacaa ggaaactttt 1200

aagtgtaatt tgttcattat ttggatggct tttggggatg cacaggtgag cacgagtgga 1260

attacattgt gttttgtttg tttctttttt ttttctgaag agatgtggtc cttctctgcg 1320

acccaggctg gagtgtagtg gtgggatcat agttcaatgt agcctctaac ttctggtctc 1380

caacaatact cctgcatctg ccatctaagt agctggaact accgttgtgt gccaggaggt 1440

ttggcttggc ttgagtactt attaaaccac acactttttg caatatttaa tgtatagtaa 1500

taatgtctca ataagactac tacaaatcaa tgaatgaata atttgttcag tacagattca 1560

tggaaaaata gacactaaca tgatgaatgt ctgacattta tgaaaataca actgcatgaa 1620

atgtgcttct ctttacattc attaggtaaa cacaatagtg catacacatc acaccgtgct 1680

ttcattacag gaagaaagat ctgaaaatgt cactggggtg aaccacattg tgctgggctt 1740

ggttcaggga gcagtcaggc ccagtgttgt gaccttcaac cacagaatcc ttaaaaaata 1800

ataaaagagg ctcccccaaa gtccccatca gttcccggac tcgctatgtt tctggatcgt 1860

atcagtgcat ccggagctcc ctggtggctt tagtgattcc ttgcttgcca tgctgaggtc 1920

tccctgtaga ttatgttggg ttttctgagg ccgttttgct atttaagacc cactccctgg 1980

cacagagcga ttccctctaa acctgataga ggttctgaac taaaaataac attaagtgaa 2040

tcctggtgtg tctgaactca agtgattgtt acattaagct gctgttgcaa tctgtttcct 2100

cacctgggaa aagaggagcc aggacatagt gagttgaggc cccaggaaga taactgaatt 2160

ctcagagggc acagccagca tcctcctccc agggagagtc taaaagactg gggcctccct 2220

catccctttt cacttctcca tacagaggca ccacccccat gcaaatctca cttaggcacc 2280

cacaggaaac caccacacat ttccttaaat tcagggtcca gctcacatgg gaaatacttt 2340

ctgagagtcc tggacctcct gtgcaagaac atgaaacacc tgtggttctt cctcctgctg 2400

gtggcagctc ccagatgtga gtgtctcaag gctgcagaca tggagatatg ggaggtgcct 2460

ctgagcccag ggctcactgt gggtctctct gttcacaggg gtcctgtccc agctgcagct 2520

gcaggagtcc ggctcaggac tggtgaagcc ttcacagacc ctgtccctca cctgcgctgt 2580

ctctggtggc tccatcagca gtggtggtta ctcctggagc tggatccggc agccaccagg 2640

gaagggcctg gagtggattg ggtacatcta tcatagtggg agcacctact acaacccgtc 2700

cctcaagagt cgagtcacca tatcagtaga caggtccaag aaccagttct ccctgaagct 2760

gagctctgtg accgccgcgg acacggccgt gtattactgt gccagagaca caatgagggg 2820

aggtgagtgt gagcccagac acaaacctcc ctgcagggag gcggaggggg cgggcgcagg 2880

tgctgctcag gaccagcagg gggcgcgcgg ggcccacaga gcatgaggcc gggtcaggag 2940

caggtgcagg gagggcgggg cttcctcatc agctcagtgc tctccctcct cgccagcacc 3000

tcagctgtcc ccaggactcc tctttcttta ttatctgtgg ttctgcttcc tcacatcctt 3060

gtggtaggaa aggaaggagg aaggcaaatt ttcctcttag agtcaaagtg tcactaatta 3120

ctaggaactt tcctacaagt tcctgaatgt cccatttttc cttcttaatt aaaaaaaata 3180

tatattctaa tacttctcac catctcttga tttgtgtcat cagttgaatt gtgctgtctt 3240

tgaaattcaa atgctgaaac cttaaatcca attgatctat attggaattt taaggatgga 3300

attaaggtta aatgtgatca taagtctgag attctaatgc aatagatctg ttgtctttat 3360

aagaagtgga agagtcacca gagacctctc acttttcccg tgcacgcaga gaagaggcca 3420

tgtggagaca tagtggacta gaaggtgcaa gccaagaaga agccgcacca agaaccaacc 3480

ctgccagcac ctggaccttg gacattcaga cttcagaatg gtgagaaaat caatgtttgt 3540

tgtttaagcc acccactcct gttgtcttct tatgaagacc cagacagact aataccacat 3600

aactctgtta gctcctggag ggagaagcag ctccctgagg ctgggcacat ctctcagata 3660

tccatatgaa gtaggcagaa atagtagttc tcatataaaa atgtgtcatg gccctgttgg 3720

ccattttttt ttgacggagt ctcgctctgt ggccaggctg gagtgcagtg gtgcaatctc 3780

agctgactac aaaccccgtc tcctgggttc aggcaattct ccttcctcaa cctcctgagc 3840

agctgggact acaggtgtcc accaccatgc cagcctattt tttttttgta tttttagcag 3900

agacagggtt tcactgtgtt agggtggtct caaatctcct cactgcatga tctgcctgcc 3960

tcggtttccc aaagtgttgg gattacaagt gtgagccacc gcacctggcc cctgttggcc 4020

attcttgggg caatgtctct gaaaccagcc ctggtgcctg taccacaaaa ttttctttta 4080

tctttcatgt ggatataaca aatggacaat gaggaccagc tgcatggaga ctgaccactg 4140

aacatcttct gctgtctcct aagtaagtca caggaaaaca caccaacatc accaacataa 4200

ctgttttcct tcaacttcct cgaacgaact atagaaatga tcccttaaag tatagtctat 4260

tccttcaact ttctcaattt gcactgaatc ccttcctaaa ttaggagcta catagggtct 4320

gagttttgtt ccctttctcc cagtcttccc caagtatcaa ggacagaata gatttaaatt 4380

aaatttggcc gtccatgccc ccaacaccac atcggtttct aacatccttg tcatgtaccc 4440

atccttctgt gggcacccca catcaggttg cccaggaatg gccagaaggt gtcatcacct 4500

tatgggctga ggctacaagt tatacacacg tgtgatttca gtcacacaca ctctactgca 4560

ggacacacct gtgttctgag gaactcaggc gcctgctgat ctcaggtctt ctctaataaa 4620

ttacacacct cttatgaatg aaggtccaga tggccccatc agctgcagag cagtggatta 4680

aagctcatgg gtgggtcagt caggcagaag tcagacaatg ggataataga agtcagacaa 4740

tagtcactga ggttcccaat gtattttaat ggaataaaac aatattagca acaaaggggc 4800

agtaaagaag actggctctt gaaggtattt aaggaccagg aactttattt ggggggaaag 4860

tgagagacac ttttacatgg aaagccctaa agcacataca gcagctgaca gagtggccac 4920

tgtgcacatg agggctgagg agacggatga taggttacgt tgctccaaga tgtccctggg 4980

tgtgtgatgg ttggactcct catgcatatg aaaataagag ctggactcag ggagaaacaa 5040

gggccatacc ccatagaaaa agacaagtct taactgcttc cagctgagga gggatgctgt 5100

ttggggaaga tctctcttgg aggtctaagg gaccccagga aaagggagcc attatcccag 5160

gcttcagttg catgaccatt tggagtttga tggtctgaaa atgagaagag gcaaatctgg 5220

ttattagaag acatgtatga aaaccaaaca aggtggcaag gacagcttga aagaaaattc 5280

caaggctgct gacattccta gataactgca gctgtagtta tgcctgctaa ggtttgggcg 5340

catggggctt ggcttttgtc agctccctgg gatttatttt cccaaacaaa gaaacctcca 5400

ggttaggggc accctattca ttcccatcac ctggcatgat ttaaaggata attgcttaga 5460

attaaaatat tgatccagat tttttatatt ccccatcgct ttttgtttct tctgggctgt 5520

agccagagat cattgattgg cgctcaggaa taagcagagt tagtctaaaa tgcaggcaaa 5580

tacttaaaca actgaagaga ttagaattta aagacaagtg tatgatatgt tttgaaatac 5640

aatgtttctc tttccagttt tggtttttgt cggcagcaaa taatgataag actgagttgt 5700

ttgcaaaata aactttagtc ttaaacttgg cctgattatt tgcataaagt gcagcaagaa 5760

tattaataat aattctgtag gaaaagcctg caagcaccag gagcttcaca gtctaacact 5820

atgagcacgt gcatcctcac gcaactcact gaatatttcc aagccagcct gttccgatct 5880

taaatgccat ccagtggcat ctgccccagg tacactaata catgggtcct gcttctctct 5940

gcagcctcct ctctcctcag atttcaggtt ttgtttattg tttgttttct ctctgacatc 6000

aacacagata tgttgaaggt tttctttttt ttatttgtag ttgttcagct ttgttgttaa 6060

tgaggtcaga ataagctcat agtttacaca tttttacatt cccatgccga gtagctgctt 6120

ttctctatca aatccattaa ctgagagaac aatcacattt cgttacaggt gaacagttaa 6180

atagtttggc atatatttct gtgctggaat ctaatgcagc ttgaaatcaa gtcatgcctc 6240

actcattgaa aaaaacatgg ctaaattctc aaagaattgt gctgagtgaa agaaactaag 6300

gaattaagag taaattttac atgatacatt tgtagaaatt ttagaagatg ccactattat 6360

aaattaacat ggagaagatt taaatgtttc tgagaatatg ctattgggag taatggggat 6420

gtgagttaaa tttcagagga ataagagaaa gatttaggga ttaatttttt caaaccttga 6480

ttgaagtgct gagtaaatgg ttgcaaacat aggtctacat ttttcaaatc attcaccata 6540

aatttgaatt atttattaat tacactcgaa taaagcaata aagaaactga tgagataata 6600

tttgactgaa ttgcatcaat aaatagatcg atattaacac aaggaatata actgatttcc 6660

aaaaacatac acatgaacca tggttcactc tgcgtattta gataaattac agaaagttgt 6720

cataacagat ggggaatcct gcagacttca ctaggcatgg gccatgctgc cctggagttg 6780

tctcagggga gctgcctcct ccagaggtta gagcacaggc ccaggtaata ggactaaatt 6840

tttagatgtg ttatcttaga cacactgcac aactgctgtg ttctctatgt aaattatctc 6900

ctgtaaaata taacattgaa gcctgcatta aatatattgt gtaaatatgt aagaataaaa 6960

gaaagttatg agagctaagt gttaatcaag gcacaagcat ataagatata actatatttt 7020

cctgaatgat ggaattacta ccagtctccc ccaggacact tcatctgccc tgagcccagc 7080

ctctcctcag atgtcccacc cagagcttgc tatatagtgg gggacatgca aatagggccc 7140

tccctctact gatgaaaacc agcccagccc tgaccctgca gctctgggag aggagcccag 7200

cactagaagt cggcggtgtt tccattcggt gatcagcact gaacacagag gactcaccat 7260

ggagtttggg ctgagctggg ttttcctcgt tgctctttta agaggtgatt catggagaaa 7320

tagagagact gagtgtgagt gaacatgagt gagaaaaact ggatttgtgt ggcattttct 7380

gataacggtg tccttctgtt tgcaggtgtc cagtgtcagg tgcagctggt ggagtctggg 7440

ggaggcgtgg tccagcctgg gaggtccctg agactctcct gtgcagcgtc tggattcacc 7500

ttcagtagct atggcatgca ctgggtccgc caggctccag gcaaggggct ggagtgggtg 7560

gcagttatat ggtatgatgg aagtaataaa tactatgcag actccgtgaa gggccgattc 7620

accatctcca gagacaattc caagaacacg ctgtatctgc aaatgaacag cctgagagcc 7680

gaggacacgg ctgtgtatta ctgtgcgaga gacacagtga ggggaggtca ttgtgcgccc 7740

agacacaaac ctccctgcag gaacgctggg gggaaatcag ctgcaggggg cgctcaggag 7800

ccactgatca gagtcagccc tggaggcagg tgcagatgga ggctgtttcc tgtcaggatg 7860

tgggactttg tcttcttctg acagttcccc agggaacctc ttaaatttag aaaactgtgc 7920

ctaacaatgt cttctctatg catatgagga ccttttctcc ctggcacaaa atgcagattg 7980

acgctgacac ggatgaaaat tcctcaacca tggtcacaag gatcagagtc ctgagtaacc 8040

tcagggcttc ctggtgattc ttctccaatc agacccagga cagggacctc cgtgagattc 8100

cctgactgga acagtcttta tggatcctgg tcacagacaa tagagaggct gaaccagggt 8160

cagcgtcatg tagaacgtca cagatttcac gtctgatcct tctcctgaca cgaaagtatg 8220

caaatcagta tcagcaccga tctggtgctt cttttgttcc taatccattt actttctttt 8280

ttcgtcgttt ttctcctttt tccatttgtt tttcctgctt tttgcaaaag gaagatgttt 8340

tccctgtgag atgcagggga tgacaatttt gggagatggc tggaacatcc aatatcctca 8400

gggccgacca tcagtaagtg caggctagaa gtctcagaaa gagctgaagc tgcttaatca 8460

ccgtggagtt ttaccttctc cagttctgct ctgatggaat cagggccaag caggttatca 8520

atgataatct acctaacata gagtcaactg attccagttt caataacgtc tgttaaaaat 8580

tcacaccacc acctggatta ctgttttgtc aaatcaatac acagtattgt ccagctaagt 8640

agacccaaag acggaccgtt gcccatggag aaaaacatta acctgagttc taggttctta 8700

cagtgttaaa ggtgtaaaac tgattattaa aaatgaggct atttttcttt ttgctgttga 8760

gttgtagaag tttcttttcc attttgacat gaaaactttt tcagatatat ggcatattat 8820

ccaattctgt aagttgtagt tattttgttg ctttgcagaa tctttttcat aatctattcc 8880

cacttgttca attctgcttt tttttgtagg tgatttgaat gtaaaatcct gaaaaagatt 8940

gctaattttt tgagtgttga gagttttaca attagaggta ttacagttag atatttgagg 9000

catttggagt gaatttttgt gtttattcta acctaaaatt cttaattctt ttcatgggaa 9060

aatccagttt tcataccacc ctctttggaa gacactacaa tttagccatg ttatattgat 9120

ggttctcatg ctaaaaatca gcttgtcatc aatatgtggg tttatatcta agctctatat 9180

aggtatttaa gccaaaactt tctatgtttt taataaatgt tgaggcctgg aagtgaaatg 9240

cctaaagctt tcttcttgcc ttgttacaga tattgtacca aaatattcta accttttact 9300

attgagtgta ataatagctg tggcctttct taacggcttt tattatgttc aagttgtttt 9360

cttgtcttcc tactttgttc atagttttaa taatgaaact gatttttttc aaagtctttt 9420

tctgtgtctg atgaaatgtt actgagatat tttttcttta gttttttaat gtgtaccaaa 9480

ctgattgatt tgagaatgtt gaatcaagta tgcatctcag gaagaaattt gagttggtca 9540

tggtgtatgt cttctaaaac actttggagc ttagtttact attgttgggg attaattcat 9600

gtctactaat gatattggtc tgtagtttcc tttattgtgg tgcctttgtc tattactggt 9660

aatactatca tggtagtctc atagaaagag tttagaaggt gtatggcaga ctacctttaa 9720

aatagatttt atcagtggag aaatggtgat agttttttct tcacttttct gttgggaaga 9780

attttatgtt gtttaaaaga tattcagaat gacttaacct ggtttatgag ctttcattct 9840

attcctttct tccattcttt ttgaagaact gcttaccttt cctatttatt tttaagtttg 9900

tttttagaca tatgcaatac attttgaggt gaaacctggt ggaatttttt ccaataaatt 9960

agtaaaagac aagtcttaac tgcttccagc tgaggaggga tgctgtttgg ggaagatctc 10020

tcttggaggt ctaagggacc ccaggaaaag ggagccatta tcccaggctt cagttgcatg 10080

accatttgga gtttgatggt ctgaaaatga gaagaggcaa atctggttat tagaagacat 10140

gtatgaaaac caaacaaggt ggcaaggaca gcttgaaaga aaattccaag gctgctgaca 10200

ttcctagata actgcagctg tagttatgcc tgctaaggtt tgggcgcatg gggcttggct 10260

tttgtcagct ccctgggatt tattttccca aacaaagaaa cctccaggtt aggggcaccc 10320

tattcattcc catcacctgg catgatttaa aggataattg cttagaatta aaatattgat 10380

ccagattttt tatattcccc atcgcttttt gtttcttctg ggctgtagcc agagatcatt 10440

gattggcgct caggaataag cagagttagt ctaaaatgca ggcaaatact taaacaactg 10500

aagagattag aatttaaaga caagtgtatg atatgttttg aaatacaatg tttctctttc 10560

cagttttggt ttttgtcagc agcaaataat gataagactg agttgtttgc aaaataaact 10620

ttagtcttaa acttggcctg attatttgca taaagtgcag caagaatatt aataataatt 10680

ctgtaggaaa agcctgcaag caccaggagc ttcacagtct aacactatga gcacgtgcat 10740

cctcacgcaa ctcactgaat atgtccaagt cagcctgttc cgatcttaaa tgccatccag 10800

tggcatctgc cccaggtaca ctaatacatg ggtcctgctt ctctctgcag ccgcctctct 10860

cctcagattt caggttttgt gtattgtttg ttttctctct gacatcaaca cagatatgtt 10920

gaaggttttc ttttttttat ttgtagttgt tcagctttgt tgttaatgag gtcagaataa 10980

gctcatagtt tacacatttt tacattccca tgccgagtag ctgcttttct ctatcaaatc 11040

cattaactga gagaacaatc acatttcgtt acaggtgaac agttaaatag tttggcatat 11100

atttctgtgc tggaatctaa tgcagcttga aatcaagtca tgcctcactc attgaaaaaa 11160

acatggctaa attctcaaag aattgtgctg agtgaaagaa actaaggaat gaagagtaaa 11220

ttttatatga tacatttgta gaaattttag aagatgccac tattataaat taacatggag 11280

aagatttaaa tgtttctgag aatatgctat tgggagtaat ggggatgtga gttaaatttc 11340

agaggaataa gagaaagatt tagggattaa tttattcaaa ccttgattga agtgctgagt 11400

aaatggttgc aaacataggt ctacattttt caaatcattc accataaatt tgaattattt 11460

attaattaca ctcgaataaa gcaataaaga aactgatgag ataatatttg actgaattgc 11520

agcaataaat agatcgatat taacacaagg aatataactg acttccaaaa acatacacat 11580

gaaccgtggt tcactctgcg tatttaggta aataacagaa agttgtcata acagatgggg 11640

aatcctgcag acttcactag gcatgggcca tgctgccctg gagttgtctc aggggagctg 11700

cctcctccag aggttagagc acaggcccag gtaataggac taaattttta gatgtgttat 11760

cttagacaca ctgcacaact gctgtgttct ctatgtaaat tatctcctgt aaaatataac 11820

attgaagcct gcattaaata tattgtgtaa atatgtaaga ataaaagaaa gttatgagag 11880

ctaagtgtta atcaaggcac aagcatataa gatataacta tattttcctg aatgatggaa 11940

ttactaccag tctcccccag gacacttcat ctgccctgag cccagcctct cctcagatgt 12000

cccacccaga gcttgctata tagtggggga catgcaaata gggccctccc tctactgatg 12060

aaaaccagcc cagccctgac cctgcagctc tgggagagga gcccagcact agaagtcggc 12120

ggtgtttcca ttcggtgatc agcactgaac acagaggact caccatggag tttgggctga 12180

gctgggtttt cctcgttgct cttttaagag gtgattcatg gagaaataga gagactgagt 12240

gtgagtgaac atgagtgaga aaaactggat ttgtgtggca ttttctgata acggtgtcct 12300

tctgtttgca ggtgtccagt gtcaggtgca gctggtggag tctgggggag gcgtggtcca 12360

gcctgggagg tccctgagac tctcctgtgc agcctctgga ttcaccttca gtagctatgg 12420

catgcactgg gtccgccagg ctccaggcaa ggggctggag tgggtggcag ttatatcata 12480

tgatggaagt aataaatact atgcagactc cgtgaagggc cgattcacca tctccagaga 12540

caattccaag aacacgctgt atctgcaaat gaacagcctg agagctgagg acacggctgt 12600

gtattactgt gcgaaagaca cagtgagggg aagtcattgt gcgcccagac acaaacctcc 12660

ctgcaggaac gctggcggga aatcagcggc agggggcgct caggagccac tgatcagagt 12720

cagccctgga ggcaggtgca gatggaggct gtttcctgtc aggatgtggg actttgtctt 12780

cttctgacag ttccccaggg aacctcttaa atttagaaaa ctgtgcctaa caatgtcttc 12840

tctatgcata tgaggacctt ttctccctgg cacaaaatgc agattgacgc tgacacggat 12900

gaaaattcct caaccatggt cacaaggatc agagtcctga gtaacctcag ggcttcctgg 12960

tgagtcttct ccaatcagac ccaggacagg gacctccgtg agattccctg actggaacag 13020

tctttatgga tcctggtcac agacaataga gaggctgaac cagggtcagc gtcatgtaga 13080

acctcacaga tttcacgtct gatccttctc ctgacacgaa agtatgcaaa tcagtatcag 13140

caccgatctg gtgcttcttt tgttcctaat ccatttactt tattttttcg tcgtttttct 13200

cctttttcca tttgtttttc ctgctttttg caaaaggaag atgttttccc tgtgagatgc 13260

aggggatgac aattttggga gatggctgga acatccaata tcctcagggc cggccatcag 13320

taagtgcagg ctggaagtct cagaaagagc tgaagctgct taatcaccgt ggagttttac 13380

cttctccagt tctgctctga tggaatcagg gccaagcagg ttatcaatga taatctacct 13440

aacatagagt caaccgattc cagtttcaat aacgtctgtt aaaaattcac accaccacct 13500

ggattagtgt tttgtcaaat caatacacag tattgtccag ctaagtagac ccaaagacgg 13560

accgttgccc atggagaaaa acattaacct gagttctagg ttcttacagt gttaaaggtg 13620

taaaactgat tattaaaaat gaggctattt ttctttttgc tgttgagttg tagaagtttc 13680

ttttccattt ggacattaaa actttttgag atatatggca tattatccaa ttctgtaagt 13740

tgtagttact tggttgcttt gcagaatctt tttcataatc tattcccact tgttcaattc 13800

tgcttttttt ttgtaggtga tttgaatgta aaatccagaa aaagattgct aattttttga 13860

gggttgagag ttttacaatt acaggtatta aagttagata tttgaggcat ttggagtgaa 13920

tttttgtgtt tattctaacc taaaattctt aattcttttc atgggaaaat ccagttttca 13980

taccaccctc tttggaagac actacaattt agccatgtta tattgatggt tctcatgcta 14040

aaaatcagct cgtcatcaat atgtgggttt atatctaagc tctgtatagg tatttatgcc 14100

aaaactttct atgtttttaa caaatgttaa ggcctggaag tgaaatgcct aaagctttct 14160

tcttgccttg ttacagatat tggaccaaaa tattctaacc ttttactatt gagtgtaata 14220

atagctgtgg cctttcttaa cggcttttat tatgttcaag ttgttttctt gtcttcctac 14280

tttgttcata gtttttataa tgaaactgat ttttttcaaa gtctttttct gtgtctgatg 14340

aaatgttact gaggtatttt ttctttagtt ttttaatgtg gtgtaccaaa ttggttgatt 14400

tgagaatgtt gaatcaagta tgcatctcag gaagaaattt gagttggtca tggtgtatgt 14460

cttctaaaac actttggagc ttagtttact attgttgggg attaattcat gtctactaat 14520

gatattggtc tgtagttttc ttttattgtg gtgcctttgt ctattactgg taatactatc 14580

atggtagcct catagaaaga gtttagaaga tatatggcag actaccttta aaatagattt 14640

tatcagtgga gaaatggtga tagttttttc ttcacttttc tgttgggaag aattttatgt 14700

tgtttaaaag atattcagaa tgacttaacc tggtttatga gctttcattc tattcctttc 14760

ttccattctt tttacacctg gcctcttcgt ttttattcat atattccttc agcagccact 14820

atgaagaagt gaagtcaaga tgaagaacca tttgcttttc t 14861

<210> 8

<211> 11077

<212> DNA

<213> artificial sequence

<400> 8

acacctggcc tcttcgtttt tattcatata ttccttcagc agccactatg aagaagtgaa 60

gtcaagatga agaaccattt gcttttctgg ggagtcctgg cggtttttat taaggctgtt 120

catgtgaaag gtatgtgata tttagaaaat gatcccagat caaaggaaaa atataggaca 180

gatctgtttt tcagttttaa acatttatgc ctatgctttg gttggccagt tagccagcta 240

ctttttccta acatgctttc atctttctat ggcagttgtc tttacataga taagactctt 300

tttattcttt tttctcttat cttacttact tgtttgccta ctacaaacta gagaataaga 360

aattagctaa ctacagatag tcctgcttat atgaatgtcc atattgaatc ttaaaaatct 420

ataatccttc ccttgagtct cttaaattaa tattctggag aaatgaaata ataattactg 480

taagtacaac atggaatgca ttgggatttt atttccccaa agaggctggt attggtttta 540

acagctttcc tggatctgat cccacagatt tattatgacc ctttaacaat gtttaatgtt 600

atacaggttg cctataaatt gatgactgaa cttttgttat tttgtttact gatctttcaa 660

acaacttcag aaaatggaat acattctgaa tttagaggta cagtattaaa taattttcat 720

ttagaaggac acactaaaat ttgtctggat tgttcctatc actgagtctg tggttcttat 780

ttcaagatga ataatgacag aaatgtgatt aagaataggc cttatatata tattttatac 840

taatttagca ttaagaataa ctttctaatg atttaaaata taaaatatta gtaaagcatg 900

tacaatgtaa gtatatatac atatctaaaa atatatgata tatataatgg ggctacttcc 960

ctataaaccc tataaagttg aaagttgaaa atatcatact ttcagcttac agtgacttta 1020

tagggaggta gccccattgt agctgaggag catactgaat acatatcact ttcacagcat 1080

cataaagtag aaaaatcata agtagaacca tggtaagttg gggaccatct gtatataaaa 1140

cagctatcac ttactgaata tgatattcta ggtatcatgc taattccttt acatataata 1200

ttccatttaa ttctctcagt aaccatatga gaataaatac tattattact tctgacatac 1260

agttgagcca gttgagactt acagaggtga agtaacttac caaagtcata cagcaagcaa 1320

gagtgtaagc agaacccagg caatctgacc ctatagcccg cactcctact atcctattct 1380

gccttgtgta aggcaatcac tttttagtaa actcattatg gtcatatcta gcatatgttg 1440

ttaaatctat tttcttattt gtttgaagtg aattaaattt aacaaatatt tattgaacac 1500

ctactatgtg tcagatgctc ttggaataaa gtaaggcaca gtccctaata acaggtttgt 1560

taggaaaaat actgcctgtt ggtgagagca aatgctagac tctaagtctt ggtgaattga 1620

aagtatttgt aacttttctg acagctatgt atttctgata gatattaggc tgcctaatat 1680

ttttctatat taacttccca ggtttctggt aaggaagtaa tcatatgccg taatagaata 1740

tagacatttt ctatttaaga cttaatagtc aagtcactcc tcaacaatct atatttttgg 1800

tcaggcatta tgtgctcatt tatatttcat tgagaaaaaa cttttaagag tcatggatac 1860

acaagtagga aagaggacat catttaagtc attttacctc tttcagatgt taatgagtta 1920

tttcattacc ttgaatttac tccataatgt ttctttccaa tattacacag tgtcagaaga 1980

ttcattagga acttatgaat aatggtataa taatgcatta taaagtactt caaacaatta 2040

caagtacaat aacaatggtc ttaaaatgtt tttgtagtgg ctcttatcat taagactgaa 2100

atactgcatt aggtcctctt tgctctggga atcgtctcag gaatagactt ccagttaaaa 2160

ttataactat tctgatcatt taaactttat aacttgaatt cgtatactaa atgttttttt 2220

tttagttgag gcttaatgag aggaattgtt aaaggtcact tagctaaaat tatatgaata 2280

aagcatattt aacatcattg ttgtaattat tattattatt attatttttt gagatggagt 2340

ctcgctctgt tgcccaagct ggagtggagt ggtgcagtct tggctcactg caacctccac 2400

ctcccaggtt caagagattc ttctgcctca gcctcccgag tagctgggat tacaggtgtg 2460

tgccaccatg cctggctaat ttttgtattg ttagtagaga tggagtttca ccatattggc 2520

caggctggtc tggaactcct gaactcatga tccacccgcc tcggcctccc aaagtgctgg 2580

gattacaggc gtgagccact gcacccggcc aacatcattg ttattattaa ttagcactct 2640

tatttaatag cattacttat gtaaagctat tattgaattt ttaaaatctg gtaatttgcc 2700

aggcgtggtg gctcacgccc ataatcccag cactttggga ggccgaggag ggtggatcac 2760

ctgaggtcag gagttcgaga ccagcctaac caacatggtg aaactcggtc tctactaaaa 2820

atacaaaaat tagctgggca tggtggcaca tgcctgtaat cccagctact tgggaggttg 2880

aggcaggaga atcacttgaa cccgggaggt ggaggttgca gtgagctgag atcacgccat 2940

tgcactccag cctgggcaag agtgaaactc tgtctcaaaa aataaaatta aaataaaatc 3000

tgttaattga tccaaaccca gatgatacaa aatttccttt gtttattcct tgcaatactt 3060

atcaaggtca ggtgcggtgg ctctgtaatc ccagcacttt gggaggctcg aggcaggtgg 3120

atcacttgag gtcaggagtt taagaccagc ctgggcaaca tggtgaaact ccatctctgc 3180

taaaaaaaaa tacaaagatc tagccaggtg tgatggtgca cacctgtagt cccagctact 3240

tgggaggctg agggaggaaa atcacttgaa cccaggaggc agaggttgca gtgagctgag 3300

atcgtgccac tgcactccag cctggatgac agagtgagac attctggcaa ataaacaaac 3360

aacttatcag acctctttgt ctaatattca gattagacaa taaatgttga cattcaaagt 3420

taaggcttta atggtcatat gtgtatttat tcatttattc attttttttt ctgtatacag 3480

gcatattcaa ttgagattgt acctcataat atttctaaat tgcttttgca aatttacagc 3540

actttatatc tatatatact ctatctttta aaataattct ttttttcctt ttttttttga 3600

gatgaagtct cactctgttg cccaagctgg agtgcagtgg cacgatcttg gctcactgca 3660

acctccacct cccaggttca agtgattctc ctgcctcaga ctcttgagta gctgggacta 3720

caggcatgca ccaccatgcc cggctaattt ttgtgttttt agtagagatg gggtttcact 3780

atgttggcca ggccggtctt gaactcctga ccctgtgatc tgcccgcctc ggcctcccaa 3840

agtcctggga ttacaggcat gagccactgc gccctgctta aaataattct tataagaaat 3900

atagcctttt tttaaattga atcatgtact atgataatct gattgttatt atgaaaaggc 3960

agataattgt ggtagagaat atttaaaagt cttacaaaat aaggttatct tggatgaaaa 4020

catgcctgtt cttaactata atacaattta tacaaaaaaa atttaagtac aaatgagagt 4080

ttaggaagga tgcgaaaatc agaattcttt caacccagat tcccatagac tggttcaaga 4140

aaaaagtgaa aatccctttt agagtacctt tggatttcct ttactgtgat gtaagattct 4200

gcttagagaa caaatggaga aagctgagac aaaccaacca taaaactatt tttctgcagg 4260

caagaaagat aaaataactc aatacaaaat atataaattg acctttgttt tcctttcttt 4320

ttaatacgtt tacctttcaa gcccaagaag atgaaaggat tgttcttgtt gacaacaaat 4380

gtaagtgtgc ccggattact tccaggatca tccgttcttc cgaagatcct aatgaggaca 4440

ttgtggagag aaacatccga attatgtatg tggcattcca tattcctttt tttcctgttc 4500

taagcagaga tactttctga cactgagcat tgtttgaatg taacacctgc ctctttactc 4560

ccctttttgc tgtgtagcac tttacatgag taaagaattg agtcttatga atgagcttga 4620

ggcaatttta ttgaaattag gggtaagaat gaaagattac agccaatcta aaatcagtgg 4680

gctacatcaa attataaaca aaggttgtaa tctagtcatc aacatgcact gtttatatgg 4740

agttatttct attcatttat ttttgtttgc ttgtttttga gacagggtct cactctgtca 4800

cccaggctag agtgcagtgg caagattact gcttactgca gcctcgaact cccaggctca 4860

agtgatcctc ctcagtagat gggactacag gcatgcacca ccccacacct ggataatttt 4920

ttatttttct ttagagacag ggtctcacta tgttgcccag gctgttctcg aacttctgag 4980

ctcaagcgat cctcctgcct tggcctccca gagtgctagg attacaggcc tgaaccacca 5040

cgcctggcca agttatttca atttgaatca gaactttcag ttgcctttga tcagttcaaa 5100

ctgaaacttc agataacact aaagccttat tttgtaccat atatagataa ctgagtcttt 5160

tatactgtaa ccagatgtct gcatcgagat aaggcagact gatcacatta atttgatttg 5220

atctcacttt tagattggat ttcagatatt catttcattt cacacacagt ccagagttat 5280

atgctataca gctctttttc aatagcaaga atgctaatac tatttgagaa attagatttc 5340

tattggatcc aatcagttat aagttttcct tgtaaaccac tcctacctta atagtttttc 5400

cccaatcaaa tctcctatag aaagagaaca gagcattcca ttccattcaa tcaatggaaa 5460

aagtataatg tgtttaagaa ttttgtttta agttctgtct gggacactta atagttgtgt 5520

gtttttgtat gcaagttact aatctacttt gaacttcaac tttttcatct ttaaatgagg 5580

aaaaaaatca tgcttaactc ataggattat tgtaaaaata aatagagaat attcctttta 5640

gagcttatat atttctggga catattaaaa aatatttact attatttatt cacctaatct 5700

atgtgccaat tattgtcacc atcctgctta aaagtcttgt gatggtttcc ccaacattga 5760

ccttaggata tattacaaat gccttggcct gatctacaaa gcttagcata actctcaaga 5820

tcatccattg ccacttttct tttacttcat gtttaaaaat tcagcactac tggggctggg 5880

cacggtggct catgcctgta atgccagcac tttgggaggc cgaggcgggt ggatcacctg 5940

aggttaggag tttgagacga gcctggccaa catggtgaaa ccccgtctct actaaaaata 6000

caaaaattat ccgggcgtgg tggcaggtgc ctgtaatccc agctactcgg gaggctgaag 6060

caggagaatc acttgaaccc ggaaggcgga ggttgcagtc agccaagatc atgccattgc 6120

tgcactccag cctgggggac aagagcgaga ctttctcaaa aaaaaaaaaa aaaaaaaatt 6180

cagcactgct gtagtgtctc tatttcccca cattcacagt gcttttacat ctgctagttc 6240

ctctgtttga agaactctgt cccacccctc ttagcctagt taactccttc tcaatgtgca 6300

tgtcttagtt cagccatcac ttcctctagg aggttttctt tgaccactca gatcctgccc 6360

acatagcttc gtgttaggga tagaatagct ggagtagtgg gtaattggaa agaatggcat 6420

acatgacact ggcacttaag ccatgttgaa gagtgtctat cttacgttta aacctccagg 6480

agatttttaa aatctcctcc atgatcttct aattcatctt tttattttat gaaatgattc 6540

tttttatatt acattgttta ctgaaatcag ataattcaga tatttcatga cactttgcct 6600

gatgtgttga tcttctgttc tgatttctga aagtatattg ccattgctgt gccattatgg 6660

tattattatt attattctta tcattatttt gagatggagt ctccctctgt cacccgggtt 6720

ggaatgcagt ggtgcgatct tggcttactg caacctctgc ctcctgggtt caaacaattc 6780

tcctgcctca gcctccccga gtagctggga ctacaggcac gcaccaccat gcccagctag 6840

tttttttttg tatttttagt agagatgagg tttcaccatg ttggccaggc tagtctcgaa 6900

cacttgacct caggtgatct gcccaccttg gcctcccaaa gtgctgagac tgcaggcatg 6960

agccaccgtg cctggctttt tttttttttt tttttttttt tttttttgag acagagtctc 7020

actctgttgc ccaggctgga gtgcagtagc atgatcttgg ctcactgcaa cctctgcctc 7080

ccaggttcaa gcaattcttc tgcctcagtc tccctagtag ctggaactac atatgcactc 7140

ctcatcgcct ggctaatttt tgtattttta gtagagacaa ggtttcatca tgttggccaa 7200

gctgggcttg aactcctgac ctcaagcaat ccacctgcct cagcctccca aagtgctggg 7260

attacaggcg tgagccacca tgccgagcct gccattatgg tattattccc aactccatta 7320

cttactaaaa tgatgtgact ttgggcaagt tacttaacta gcctttttcc taaactttaa 7380

aaaaagggat aattatgcat gtatgaattt atacacatat actttatagg gttgtgagga 7440

ttaaatacaa taattcataa aagaacagtg tttggcttat agtagcctct cctttttctg 7500

atattactag agtctttaca gtcttattaa gaatagtttc attcattcat tcaacgaatg 7560

tttatttagt aactgccaaa tattaagggt tggtggtggg gataaaacag tttatttaaa 7620

atatagtcca tttcttaaaa gaatttatag tctaacagga aaccgtaaac aattaaaacg 7680

gcaattacaa ttctgtattg taagtgctat gataggaatg ggcatgggtt gttataggag 7740

tactgaggag gggcacccag tccaatcctg ggggagagga gaagggtggc agtgggaggc 7800

aggaaagggt gcttggaata agtggtggga tggatgatgg ctgttagctc aataggagtt 7860

tgctggctaa atgtgtgtgg ggagtgagag aattaatagg tggggcaggt cattccagga 7920

aaaggaaaca ggattgctgt gacgattata tgagatcatc acgtaatatg cttagcatag 7980

taccaggcac actgtaacca gtattatatg ttagaactgt tattgttaag atgggagatg 8040

agaaaagcat ggtacgtttg tggaattgca agtgattgtt tacagctggc atgcattgta 8100

gatggtgaca ttgtgagaga tgaagctgga gagttagggg catattagga tacgtgcctt 8160

ctatgctatt tgaggatacc ctgttatctg tggagagaca ctagaggttt taagcagaga 8220

aggaaattaa gcttgcattt ttagaaagga tgtttggcaa taatgtggcc agtggtttga 8280

aggggtcaag acaaaaggaa gacattgtta caatctagga gaggatagtg gtgtcctgaa 8340

ttaaggcagt aataatgaga agagaggcaa gtggatggat ttcacacatg aaaataatta 8400

gaatcaactg gactgtgaga ttgattagat atgtgacagg ggaggattca ggtatgatag 8460

ccaggttttt ggcttggatg gtatttataa atactgagtt aggaaacaca aggtttttgt 8520

tgttcctttt ggtagggagg gctgcacatg agaaggaatg atgacttaga tgaaaaaaat 8580

gttaaagtta atgaaatcac ctgaactcgt tgacaatcat ctaatttttc atgggacatc 8640

acgatgaaaa agttccagct ttgctactta attgttgtgt gaacttgggt ggatcactaa 8700

cctctcctat ttatgatttc ctctcctcta aaatctggat aataatatct gtctgtccat 8760

ttcacatata ttgaacatat tccatatgcc acatacctag ctaggcactg ggaatacata 8820

aatgtttttg cctcaaggaa cttacatagc ccagggaagc aaccactctg tagacaagca 8880

actatgatac ggcatttgat ataataaata tatatgagca ttaatgagac tgcagaggaa 8940

gaagtttgtt gaagggtggg gataacatca atataattat tacagaggta aaacgtgagt 9000

tgagttttaa aggaaaagca aaaatttgcc aagtcagcaa agatagttga cagcggagtt 9060

gttgtgaggt ttattttgaa tgagaaatat ttaaaatata gcaagttact ataaaaatct 9120

gagtaacggt attactctag agtgcttata gagtactaaa tacaataagt actgttctga 9180

tgaaatagtg gccgtatact cattaccaaa aataacaata tctgcatttc attgttttaa 9240

ctttgttttc tttcttttct tttagtgttc ctctgaacaa cagggagaat atctctgatc 9300

ccacctcacc attgagaacc agatttgtgt accatttgtc tgacctgtaa gatatatttt 9360

tttccatagt aatatagatg tggaagttaa tagcttttaa ttttaacctt gttagtaaga 9420

atgtttttaa aaatatgttg gagtataaac atttacaaac ataatctgaa cttttgaata 9480

cattaattcc tatgttaatt attaggtatc ataaattcat aaaactttgt cacagataaa 9540

atttagctat acattttttc taaagaaaaa atcattggca ttcatagaaa ggccaatttc 9600

tcttaatagt tcaataagtg gatttgatct tataaaaagg caggtgtttc tttggaaatg 9660

acagactcca acatcaattt ttttaaaaat tctccctttc ttgtcactat aaataacttg 9720

tttagacaga tatacagttg ggaataagcc taacacagta gaaattgctg tatggtgtag 9780

ataaaacaat catattatca tatcattaat tatattgctt actttcaact aatatatatt 9840

aaagattgga aaatcccata agctattctg tattgtagag ctgcttatgt ctgaaaggag 9900

tcatcccttg ctgtcatgtc agagctgcaa gaactaattg attttggatt gaaatgtgta 9960

gtcacatttt gagacagcat ttgaggggat tgtctaatac atatatttgc ttttcagctg 10020

taaaaaatgt gatcctacag aagtggagct ggataatcag atagttactg ctacccagag 10080

caatatctgt gatgaagaca gtgctacaga gacctgctac acttatgaca gaaacaagtg 10140

ctacacagct gtggtcccac tcgtatatgg tggtgagacc aaaatggtgg aaacagcctt 10200

aaccccagat gcctgctatc ctgactaatt taagtcattg ctgactgcat agctcttttt 10260

cttgagaggc tctccatttt gattcagaaa gttagcatat ttattaccaa tgaatttgaa 10320

accagggctt tttttttttt ttgggtgatg taaaaccaac tccctgccac caaaataatt 10380

aaaatagtca cattgttatc tttattaggt aatcacttct taattatatg ttcatactct 10440

aagtatcaaa atcttccaat tatcatgctc acctgaaaga ggtatgctct cttaggaata 10500

cagtttctag cattaaacaa ataaacaagg ggagaaaata aaactcaagg actgaaaatc 10560

aggaggtgta ataaaatgtt cctcgcattc ccccccgctt tttttttttt ttttgacttt 10620

gccttggaga gccagagctt ccgcattttc tttactattc tttttaaaaa aagtttcact 10680

gtgtagagaa catatatgca taaacatagg tcaattatat gtctccatta gaaaaataat 10740

aattggaaaa catgttctag aactagttac aaaaataatt taaggtgaaa tctctaatat 10800

ttataaaagt agcaaaataa atgcataatt aaaatatatt tggacataac agacttggaa 10860

gcagatgata cagacttctt tttttcataa tcaggttagt gtaagaaatt gccatttgaa 10920

acaatccatt ttgtaactga accttatgaa atatatgtat ttcatggtac gtattctcta 10980

gcacagtctg agcaattaaa tagattcata agcacaccca ccaaggctcc ggatgtgttc 11040

cccatcatat cagggtgcag acacccaaag gataaca 11077

<210> 9

<211> 7426

<212> DNA

<213> artificial sequence

<400> 9

gtattctcta gcacagtctg agcaattaaa tagattcata agcacaccca ccaaggctcc 60

ggatgtgttc cccatcatat cagggtgcag acacccaaag gataacagcc ctgtggtcct 120

ggcatgcttg ataactgggt accacccaac gtccgtgact gtcacctggt acatggggac 180

acagagccag ccccagagaa ccttccctga gatacaaaga cgggacagct actacatgac 240

aagcagccag ctctccaccc ccctccagca gtggcgccaa ggcgagtaca aatgcgtggt 300

ccagcacacc gccagcaaga gtaagaagga gatcttccgc tggccaggta ggtcgcaccg 360

gagatcaccc agaagggccc cccaggaccc ccagcacctt ccactcaggg cctgaccaca 420

aagacagaag caagggctgg gctgtgaggc aacccccacc tccccctcag agcacgttcc 480

tcccccttca ccctgtatcc acccctccgg accctcccca tctcagtccc tccgctccct 540

ctctctgagg cccatctccc aatacccaga tcactttcct tccagaccct tccctcagtg 600

tgcacggagg cagcttgccc agcaaaggtg actgtctagt gggcttccca cagccaagct 660

cccaccccat gctgcggccc ctcccttctt cctgcttggc tgcctgtgcc ccccacctgc 720

ctgtccacaa cccagcctct ggtacatcca tgccctctgc cctcagcctc acctgcactt 780

ttccttggat ttcagagtct ccaaaggcac aggcctcctc agtgcccact gcacaacccc 840

aagcagaggg cagcctcgcc aaggcaacca cagccccagc caccacccgt aacacaggtg 900

agaagcccct tccctgcaca ctccaccccc acccacctgc tcattcctca gccgcctcct 960

ccaggcagcc cttcataact ccttgtctga gtctccaagt cacactttgg taaggagagg 1020

gacactgaac ggacctctaa caaacaccta ctgccagcca gccccagtct gggggccagc 1080

agatgccaaa caaccagcag actcccagag cagacctggg ccggctccct ggcccatgga 1140

cccagctctg cctcgctgag ctgaggcatg ggctctcagc gcagcctcac atagagccac 1200

cctgccgagg cagtccggct tgcagactca caggtcactt gggccgcagc agcccctccc 1260

cgtgaccctc gcctcccgcc cgccccagcc tggctctctc caagtgttgg atcttggtgg 1320

ccagcctgct tctcaccctc accctgcctg ccacctcaga atggcagggg aaagagggcc 1380

ctcaccaaga actttatctg agaagtctga ggcttgtgac tctgacctgc ctgagatgtc 1440

catgtggccg gggggacggg ttcagtgttc gggagaactc gggtacgtgc ctgactttct 1500

ctgagtaggg caggaagctg ttaggagaag cagcagtgag gtgggctgga ccaacaggca 1560

gaatgactgt ccctcagcca ccctctggga tgtgggtcaa gctctgacaa aggcatggca 1620

cagccatggt ggcccctgct tggatgagtg gccacggtgc cctcaccctg ggccagaatc 1680

tgcctccact ctgcaggtgc agaaacacga cattcccgtc tctaaacaca cctagctcct 1740

aggcttgggg tgggcctatc aaatgcaggg agatggacac agcacaaggg ccagagcttc 1800

ccatgagaaa ggtgagggca gctgctccct gacccgggca tctgcacttg tccctctcca 1860

ccctcctcat gggcagtgga gactcagcaa caaaacaagt tgagtgcatt agcagccagc 1920

tctggagcca agtcactcac cccacggcct tggctgctgg tggaggggcc ttcccctggg 1980

cagcctccaa gaagacagcc aagtgctctt actcagacca cggcgctgct tcctggcacc 2040

tcgatttccc acaacaacat ggggtgcaga caggctaggg ccccctgccc tggggcctgg 2100

acggcatcca gttaaagatg acccttcacg ggcggtgcct gaggtgtgct gacctcagca 2160

gctaagccct caggtctggt ctgcactgcc ccacctggag gacccaactg acccagacac 2220

agccagggtt atggcatgac cccgtggacg gtgacccaca ggccagatgc agccgggggc 2280

tgttttgtgt ggcctagaaa tgtctttaca gttgtagtgg gatggaggag gaagaggaag 2340

agaggagggg agaggaaagc agggaagggg aaaaagagga gttcaatgca accccaaaag 2400

ccagaacagt tttgagctga aagaacaagg caggaaacat cccagtacct gacttcaaaa 2460

catactataa agcagttgta atcaaaacag gatcataaaa acagacacac agacccatgg 2520

aacagaaaag cgagcccaga aataaatcta catgcttgca gtccattgat tttcaacaaa 2580

ggcaccagga aaacacaatg gggagaggac agtttcctca ataaatagtg ctggggaaac 2640

tggatatcca tgtgcagact aatgaaacta cacaaaaatc aattgaaaac agtctaggcc 2700

aggcgcggtg gctcatgccg gtaatcccag cactttggga ggccgagaca ggcggatcac 2760

ctgaggtcag gagttcgaga ccagcttggc caacatggcg aaacccggtc tccactaaaa 2820

atacaaaaat tagcacatgg tggcctacgt ctgttatccc agcttttcag gaggctgagg 2880

caggagaatc gcttgaatcc gggaggtgaa ggttgcaggg agccaagatt gcgccactgc 2940

attccagcct gggcaatgga gcgagactgt ctcaaaaaaa aaaaaaaaaa aaagaaaaga 3000

aaacagtcta aaggtttaac tgaacagata aagctactag aagaaaacat agggggaaaa 3060

ctccatgaca ttagtctgag caacgatttt tggatatgat cccaaaagct caggcagcac 3120

tagtcacaaa agccaagata cagaaccaac ctaagcaccc ctcagcagat gcacaggtaa 3180

agaaaatgtg gtacgtatgg ggcacaatgg aatacgattc agcctttaaa aacagtgaaa 3240

ttctgtcatt ggcaacaatg tagatgaacc tgaaggacac ttatgctaag tgaaataagc 3300

caggcacaga aggagcaata ctgcatgatt gcacttacat ctggcaggtt aaaaaggcaa 3360

actcttagag gcagacagta gagaggtggt gccagggagc gggcactggt ggctggggag 3420

atgttggtca aagggcacaa aactgcagtt gggaggaatt agttcaggac atcccttgta 3480

catggggaca gtggttagta acaacggatt gtatccttga aaaccgctaa gaaaatagtt 3540

tttaagtgtt cttgacacaa aaagtgacac gtatgtgaga tactgcatgg tcattagctg 3600

gatttagcca ttccacaatg tacacatatt tcaaacattg tgttgtatat gataaacatg 3660

tataattttt gtcaattaaa aatttttagg aagaggagga gaagagaaga agaaggagaa 3720

ggagaaagag gaacaagaag agagagagac aaagacacca ggttttttct gacccctggg 3780

ctatcaaaac acctattgcc caataactag ttggccgttg gtgccctaaa ctattgaagc 3840

gattgctgtt atgtggatgg gccccggaca cttagaaact cgtgacccct gaggaccccc 3900

acgaggacag tcagggtccc cccgaactca gggagcactg aggaaggagc tcttagaggc 3960

gtggggcccc tcaggcccct cagagggctc tgccacatgg gtcaggggca ggctgagggg 4020

gagtcccagg ctccatgccc agcctctgtg cctctgacca gggtgtcccc cacaccgcct 4080

cctccccagt gccctccact ggccacacct ggccagaagc tggggagagg agagcacagt 4140

ggttaagtca gtccctgcag ggagacggca ccagaaaaac ctggcctgtg gatgagtccc 4200

ggcctggcag ccacagagca gagagctctg gaagcaacga aggcccgagt ctgctcaggg 4260

aagagcgggc agcagcccca gggccggaca gtgaccaaga gtggcaccgc ccatggctca 4320

acgggtcttt gcccacagat cccccagccc ctggagacag ggtctgtgtg cctggccgtg 4380

caggcaggca ccacactcag ggggaggcca ctgtggagct ctgtgcagag ccccgggcgg 4440

gagcctactg ctcccgaagg tccggccaca gctgctctcg tttgctctcc cctgcagagt 4500

gtccgagcca cacccagcct cttggcgtct acctgctaac ccctgcagtg caggacctgt 4560

ggctccggga caaagccacc ttcacctgct tcgtggtggg cagtgacctg aaggatgctc 4620

acctgacctg ggaggtggct gggaaggtcc ccacaggggg cgtggaggaa gggctgctgg 4680

agcggcacag caacggctcc cagagccagc acagccgtct gaccctgccc aggtccttgt 4740

ggaacgcggg gacctccgtc acctgcacac tgaaccatcc cagcctccca ccccagaggt 4800

tgatggcgct gagagaaccc ggtgagcctg gctcccaggt ggggagacga gggtgcccac 4860

agcctgctga cccctacgcc tgccccaggg ccatgacccc agctgggccc cagcagcacc 4920

ggtcatcctc cacaggaaag gagaagggag gcaccagcac cctggccggc cccacttctc 4980

tcccagtgcc cccgtggcca gagcctgaca gcctccccca cctccccgca gctgcgcagg 5040

cacccgtcaa gctttccctg aacctgctgg cctcgtctga ccctcccgag gcggcctcgt 5100

ggctcctgtg tgaggtgtct ggcttctcgc cccccaacat cctcctgatg tggctggagg 5160

accagcgtga ggtgaacact tctgggtttg cccccgcacg cccccctcca cagcccagga 5220

gcaccacgtt ctgggcctgg agtgtgctgc gtgtcccagc cccgcccagc cctcagccag 5280

ccacctacac gtgtgtggtc agccacgagg actcccggac tctgctcaac gccagccgga 5340

gcctagaagt cagctgtgag tcacccccag gcccagggtt gggacgggga ctctgagggg 5400

ggccataagg agctggaatc catactaggc aggggtgggc actgggcagg ggcggggcta 5460

ggctgtcctg ggcacacagg ccccttctcg gtgtccggca ggagcacaga cttcccagta 5520

ctcctgggcc atggatgtcc cagcgtccat ccttgctgtc cacaccacgt gctggcccag 5580

gctggctggc acagtgtaag aggtggatac aacccctcgc cgtgccctga ggagtggcgg 5640

tttcctccca agacattccc cacggctggg tgctgggcac aggccttccc tggtgtgacc 5700

gtgaatgtgg tcaccctgaa cagctgccct ctctggggac atctgactgt ccaagaccac 5760

agtcagcacc tctgggagcc agaggggtct ccagagaccc ccagatgtca ggcttgggct 5820

cagtgcccag cgaaaggtca gccccacaca tgcccataat gggcgcccac ccagagtgac 5880

agcccccagc ctcctgccag gcccaccctt ttccgccccc ttgaggcatg gcacacagac 5940

cagtgcgccc actgcccgag catggcccca gtgggatgtg gtggccacga ggggctgtac 6000

acacagcagg aggctgtccg ccctgctcag ggcctgctgc ctatgcccca gctgtccagc 6060

caagggaggc atggaagggc ccctggtgta agctggagcc aggcacccag gcccccggcc 6120

accctgcaga gccaaggaaa ggaagacacc caagtcaaca aggggcaggg ctgagggctg 6180

tcccaggctc ttttggcccg aggggctgcc agcagccctg acccggcatg ggccttcccc 6240

agaagcgacc ctgtgaggtg gcctcacaga gaaccccctc tgaggacagt gtctgaccct 6300

gcctgcctca cacagatggg ccccacagca gtgggcaacc tggggggcag cagcccaacc 6360

tgaccctgca gggactgccc cctgcagcag cagctgcttc tcagtccccc aacctccctg 6420

tccccgccag agggtcttcc ccgaagctgc agccccaacc catggctgcc cacctggaac 6480

cgggactccc tgtccactgc cccctcccct tcggggcccc atctgtgctg gggcccaggt 6540

tcggcctaca gattcccatc attgccatgg cctcctgacc ttgcctatcc acccccaacc 6600

accggctcca tgctgaccct cccccaggct cccacgccca gctggccggc catccccagg 6660

cacagacagt ctgggatctc acaggttagc ctggaccatc cacctggcca gacctgggag 6720

aggctggaag ctgccctgcc accatgctcc agggccccag gttgcagtac tatggggtga 6780

gggtgtgtgt gcacacccgt gtgtacctag gatatccgag tgtacccttg tgcccccaag 6840

cacaagtctc cctcccaggc agtgaggccc agatggtgca gtggttagag ctgaggctta 6900

tcccacagag aaccctggcg ccttggtcaa ggaagcccct atgcctttct tgcctcgatt 6960

tcccctcttg tctgctgagc cagcaggggc cacgtcctgg gctgctgtga ggaggaagtg 7020

agttggtgct aggaggggct cctgtgtgtg catgggcggg aggggtgcag gtatctgagc 7080

accccggtct ccacttgaga gagcagggca ggagctccct gacccaccca gactacacac 7140

gctgtgtcca cgtgtctcac attatctgtg gcagaggatc cggcttcttt ctcaatttcc 7200

agttcttcac aaagcaatgc ctttgtaaaa tgcaataaga aatactagaa aaatgatatg 7260

aacagaaaga cacgccgatt ttttgttatt agatgtaaca gaccatggcc ccatgaaatg 7320

aagcagctgc aggagtcagc cctggacctg aagagcacac acttaccctc ttcacacatt 7380

acattctgct gggcctttat taaccacatt tctatgaacc tagtca 7426

<210> 10

<211> 15081

<212> DNA

<213> artificial sequence

<400> 10

agcagctgca ggagtcagcc ctggacctga agagcacaca cttaccctct tcacacatta 60

cattctgctg ggcctttatt aaccacattt ctatgaacct agtcagggtt gggtttcaag 120

tgtatggttg ccacggctat cagagttgaa gtcagcttct cctgttcaca aaaagttcag 180

gttcctccag tgatacccac ttttgtgtcc cggtttggct cttcccattt ctttctcccc 240

agagagagcc tgtctctttc atctgtggca ggtgcatcct gctgacactt ttacttggtg 300

attgtttgtg gggtgaaggg gcttggacac agggggatgt tctccaacct tctgaccaag 360

catccttctt agctatgggt ggtgagagtg gctctgaagc atggtcttcc aagcgttcct 420

gttccttccc ttccccaagt cagagtgtct ctttctagcc acagtggtct tccatcagtg 480

tcctcagctt ctgacccact gtccttactc cacagactca gggtttcatt cctcaggaaa 540

gagacgggag gtgtttctgg gtagagtctc cttggtgtcc tctgtttcct tctgttctag 600

ttgattctac cagtgcctga aggacacaag atttaataaa tgtctcccac atatcgtgaa 660

gagggattca gcattgaacg gagctactgt tcttcctccc cagtcaacac cacaggacag 720

caggtggctg agttgtctgg ggatttcccc aattctgtag gaaaagcccg caagtgccag 780

tagtttcaca ctctcacagc gttagcacac acatcctcaa caactcatga aacatttcca 840

ggttagcttt ttcctatctt caataccata caatgagtgg cacctgcccc aggtactcta 900

ataaatggac ctagttttct ctgcaggctc cccattttct cagatttcag ggtttttttc 960

tctgcaacat caactcagat atgttgaagg gttcattttt tgtagttctt caaatttctt 1020

gttaatgagg tcagaagaag atcattttct catttttttt tttacattcc cgtgcttagt 1080

atctgctttc taaataaaat tcggaaacta agacaaaata tcaaatatcc actatttggt 1140

gcattgttaa actatttgag aaatattcat gtactgaaat acaatgaaca attgaaatca 1200

aggcatgcct cagtcacata agaatgtgag gacattatca aataattgtg ctgagtgaag 1260

gaagctaaag aattcacagt aaatctcctg tgatttcttt tgtataaatt gtagaaaatg 1320

caactattct aaattaacat ggagaagatc tgaatttttc tgagaaaagt gtggtgaggt 1380

aacaagatgg tgaaataaaa ttacagagaa gtgagagaaa aaaatttgga ggttaattta 1440

attgctaata gcacgattga agtgctgatt caaaggctgc acacatatac caacattttc 1500

catatcgtac actataaatt tgaattcact attgattgaa tttttgaata aagcagtaac 1560

aaaaatgagt atattggctg aggaagagca agaaagagat gaatattgac acttgaataa 1620

tcacggactc ctgaaaatac acacatgtga acactgattg catatttcag gtaaatacta 1680

gaaaaagcaa agtcacataa agtcgttatg acaggtggga taccctgcaa atcacactag 1740

gcatgtccca cactgccctg gagctgtctc aggggagcag tctcctccag tgtttagagg 1800

cacaggcacg gataataggg ctgacgctgt ccagatgtgt gatattggac acattgcaca 1860

actgctctgt tatgtatgta attcatcttc tctaaaaatg taacattgac acttgcactg 1920

aatatattct gcaaatatgt aaacattaaa taagatgatg actgctaatt gatcatcaag 1980

tcacaatcac ataatctgaa gttatatttt cctgagagat aggattacct ccagtgtttt 2040

ccgggaccct ctcatctgct ctgggcactg ccctctcctc cagcgtccca ctagagcttg 2100

ctatatagta ggagacatgc aaatagggcc ctccctctgc tgataaaaac cagccgagcc 2160

cagaccctgc agctctggga gaagagcccc agccccagaa ttcccaggag tttccattcg 2220

gtgatcagca ctgaacacag aggactcacc atggagtttg ggctgagctg ggttttcctt 2280

gttgctatta taaaaggtga tttatggaga actagagaca ttgagtggac gtgagtgaga 2340

taagcagtga atatatgtgg cagtttctga ccaggttgtc tctgtgtttg caggtgtcca 2400

gtgtcaggtg cagctggtgg agtctggggg aggcttggtc aagcctggag ggtccctgag 2460

actctcctgt gcagcctctg gattcacctt cagtgactac tacatgagct ggatccgcca 2520

ggctccaggg aaggggctgg agtgggtttc atacattagt agtagtagta gttacacaaa 2580

ctacgcagac tctgtgaagg gccgattcac catctccaga gacaacgcca agaactcact 2640

gtatctgcaa atgaacagcc tgagagccga ggacacggct gtgtattact gtgcgagaga 2700

cacagtgagg ggaggtcagt gtgagcccag acacaaacct ccctgcaggg gtccccagga 2760

ccaccagggg gcgcccggga cactgtgcac ggggctgtct ccagggcagg tgcaggtgct 2820

gctgagggct ggcttcctgt catggcctgg ggctgcctca ttgtcaaatt tccccaggaa 2880

cttctccaga tttacaattc tgtactaaca tttgatgtct ctaaatgcaa tacttttttt 2940

gtcctttttg tttctttgtt tttttgcaac aggagtacat atcctcagct ccacagaagc 3000

cagggtgtca ctttgggggc agaaataatc ctttcatggt taccaggata agagtcctga 3060

ggaatcccag ggaaacctgg agagtgtttt ccagttagac tcagggcaga gacctccatg 3120

ggaatctctg attagaacag gccttgagct ctgacgggag ccaagagaga ggctcgccca 3180

gggtcagagt ccttaaaacc tgatggtttt cacagctatc ccccctcgtc ttgtaaactc 3240

agactgattc agttgaccct ctttctgcta atccatttcc ttctctgtag gtttgattct 3300

cacagttcgc tttcttcttc tcttccctga aaacagacga tgtgttttct gtagtcaaaa 3360

tcccagggct caggtctgca ggacctgggt aggctacggg gactttctca ctcaccattg 3420

tccggacact cttgtcttct gtgcatggag gcatttggaa aatgaagtgg acattagcca 3480

tgaagggaat aatactagtt ttctccaata ggatattgat gtagagctga tcttgtgctt 3540

ctcacactgt ctcagagttt ggactctcac ctgtgacttt gagaagagct ggggatgggc 3600

actccattgt gctgtgagct ctgggtaacg ataattgtag aatctgccta ggcagtctaa 3660

ggtcaatact actcgtcatc aggaaagaca gctggaattc ctgggaagat ctgcatctgc 3720

cgtccaccat ggagtcccat cgtcttctgt tatgctctct ttgaatcagt cccacctaga 3780

ttatctagaa cactcttcct gacttaggaa aaataatggc aggctccact aacacctgtg 3840

ttatgccatg ggagcaacac ctaagctagt gtgtgaatga gtagatgaga ctgtggtcta 3900

gtcaaggtga caggtaaaat tgattgttgc cattatgata ttttatttta tatttggcaa 3960

tatagtcatg ctcctattat aaatattctt tgagacggag tcttgctctg ttgccagcct 4020

ggagagtgca gcagcacgat ctcggctcac tgcaacctct tccacctccc aggttcaagc 4080

agttctcctg cctcagcctc ccgagaaact gggattacag gtgcacgcca ccatgcctgg 4140

ctaatttttg tatttttagt atcgacaggg tttcaccatg tgggccagtg tggtctcgat 4200

ttcctgacct cgtgatctgc acacctcggc ctcccaaagt gctgggatta caggcatgag 4260

ccaccacacc tggcctagtt ttattaatgt ctgcccatac cagcaactac atacctatgg 4320

ggacattaat ttacatctgc agacatatgt gtaaatacac aagcctatac atacatgtgt 4380

agccatttat atttaacatt ataataaaat aattctaaaa tattttctaa agaattaaac 4440

ttaatgatga gctaaatata aattagagta atctataatt gatttcgatc attctctata 4500

gtttgcataa atggatgtct atttctaagc cttaacatag tgtattcgtc attttaaaat 4560

agtcaagaaa aaattacaaa tgttcttgtc acaaaaaaag ataaggattt gatgatttga 4620

ggtaatatat atgtcaatta actcgattca attattccgt attgcattca caaaccataa 4680

catacctttg tgccccataa atatatacaa ccaaaatttc tcaattttca atgaaatttt 4740

aattatatat ttttaaatct gatgcctctc cttggattaa gccacctcct caggcttaca 4800

gggctcttcc attttctcaa catgttgtta taccagatga gcacaaacac attaatttca 4860

ttatgcttag ctttaatttt tcaaaacaac ataaaggtga taattttacc aatagacgta 4920

ttacaaccta ctgtgcatga gaccctttct gtgcttcaag gtttcttctc aggactttat 4980

atgtattgca aattttcatt gactcaacaa caataacatt taggtttaaa ttctggattc 5040

cccaacagaa ccagtgcttc ctgctggagc tggatccatc agcccccagg gaagggactg 5100

gagtgggtca ggtgcacagg tcatgaaggg agcacaaatt ctaacccact cctcaagagt 5160

ccagtcacca cctccagatc tatgtccaaa aacagctctt cgtatggctg agtgacatta 5220

gcaacaagca cacaaccatg tttgtttttt tgttttgttt tggtgtgtgt gtgtgtgttt 5280

ttgatagagt cttgtgtcac ccaggctgga gtgcagtggg gcaatcaatc ttggctcact 5340

acgagctcca cctcctgggt tcaagtgatt ctccagcctc agcctccaag tagcttggac 5400

tgcaggcact caccaccaca cctggctaat ttttgtattt ttagtagaga caaggtttca 5460

ctatgttggc caggctggtc ttgaactcct gaccttctga tccacccgac tcggcctcca 5520

aaagtgctgg gattacaagt ataagccact gtacccagcc acaaacaagt atttttaagc 5580

aaaagacaca gtgaagagac ttcagtatga gcccacacac aaaccttcct gtgggagttt 5640

acagaacagc agtgggtgct gaggacagaa gccagcaccc aggaaccagc agggaaaccc 5700

agggggcatt tggcaccgcc tgaaggctca ggaccgttgt ggggctcagt ggtcaggcag 5760

gctcaaggtt cagcctcaga gcaggtgtag caggcgggga aaggctctga agacggagtt 5820

tagtgtcacc ttctcatttc caccactaaa caccctccag cacatctagc aggcggggaa 5880

aggctctgaa gacggagttt agtgtcacct tctcatttcc accactaaac accctccagc 5940

acatctaatt ctaatctaat gtatgggtgt tcatgtgttt agagaatatt atttatgtta 6000

tgaatctata gccatctgtg ggtgcatcaa gttaacctct tcaacctatg tggaccctgt 6060

tcattaggaa taagtccttg tatttgagga cctcacaaat taataattat gtagaatcac 6120

tttctttttc agtctccttt ccttcctctt tctttctttc tcactcacac actgacaaac 6180

acacggggtg ccataacatt aattacctga tgtattaaag aatattgatt catttgcaac 6240

ttgaccagtt tagctgttgt tcatgttgtt gtaagatcag gaacatgttt ctcagctgtg 6300

tactcctcta agctgagcag cagctttatt tgaaatacac agaaactgaa aacaatccaa 6360

atattaatca gcaccttcat aagtaaacaa attgtggaaa agtcatttat tgggatagta 6420

cccactacta caatcagtaa atgttggata cgatcaacag catggttcaa ttcacaagta 6480

catatgatga gtatagtgag ccaaagcaca tacatatgat tccgttttat aaactgtaca 6540

aagtgaatac tcattggaaa ttacataaca aagatcacta actgacttct ccatagtaag 6600

agaagcgaag gtataggagg agaaattgcg agagacaaag agaaaattga gaggcgaatt 6660

catttgtttt ctctgtgtat ggtcattagg tcaatgtttg tcaaatggtg aacatattgt 6720

gtgaagatta tatgtcagta ttacctcatt aaagttatta taaataaaag tctaatgtgg 6780

tagaaaaaga tgaagagaga aataaaaata atacaagaaa agtcatgaac tcctgaatga 6840

attaaccctt agtttttctc tattacttac aaaaacacca agatacagcc aaataatatc 6900

acgatatcat tataagaaga gtgttttgta aacctcactg ggaatttcta gctctttcct 6960

agagttaatt ttgggaacag ttggatccaa ttgtgagaaa cgcaggccgg acactgagac 7020

tggctcttat gagatgtgag ctcttgtcta tgtcacatgg tccctccata cttgggggtt 7080

tacattcaca tctgtaaatg aaggaaacat tgactctcaa agaacatatt tcatgtgcat 7140

gtaaaagtat gaatgctaat gagaattaat tacttatgaa gtataatcac ccacatccac 7200

tcttggacac agcccactct gaggcatccg ttacagaact cattatatag taggagacat 7260

gcaaataggg tcctccctct gctgatgaaa accagcccag ccctgaccct gcagctctgg 7320

gaaaggagcc ccagccctga gattcccagg tgtttccatt cggtgatcag cactgaacac 7380

agaactcacc atggagtttg gactgagctg ggttttcctt gttgctattt taaaaggtga 7440

ttcatggata aatagagatg ttgagtgtga gtgaacatga gtgagagaaa cagtggatat 7500

gtgtggcagt gtctgaccag ggtgtctctg tgtttgcagg tgtccagtgt gaagtgcagc 7560

tggtggagtc tgggggagtc gtggtacagc ctggggggtc cctgagactc tcctgtgcag 7620

cctctggatt cacctttgat gattatgcca tgcactgggt ccgtcaagct ccggggaagg 7680

gtctggagtg ggtctctctt attagttggg atggtggtag cacctactat gcagactctg 7740

tgaagggtcg attcaccatc tccagagaca acagcaaaaa ctccctgtat ctgcaaatga 7800

acagtctgag agctgaggac accgccttgt attactgtgc aaaagataca cagtgaggag 7860

aagtcagtga gagcccagac aaaaacctcc ctgcaggaag acaggagggg cctgggctgc 7920

agaggccgct caagacacac tgagcatagg gttaactctg ggacaagttg ctcaggaagg 7980

ttaagagctg gtttcctttc agagtcttca caatttctcc atctaacagt ttcctcagga 8040

accctgtcta gatctgtgat ctggatctgc tgaaactgcc tgtgtcacct tcctcacctg 8100

tgattttggg ggagctgatt gtggacactc cagtgtgtgg gatttcttga tgacagcaat 8160

tgtgtcttct gtctaggcat gtctagggct ggccatcagg aagggcaggc tggaattttt 8220

ggaaagaggc gcacctgcca tccaccagga aattttgttg tcttttgttc tgctagaatt 8280

aaatcagaca caccaaggtt aactagcact atcttcctag ctcgagaaac ttgatagcaa 8340

gcttgaataa cacttgtatg aaaccatcag agcaacacct agaatagtgt ctgatttaat 8400

aattgagact atggtctagc caaggagaca cataaaacgt gatttccaag gttggttcat 8460

attttatatt tatcaataga atctggctgg tattataaac agctttgcca aaatatgtgt 8520

gttagtgttg gtcttcggag aagcaaactc tgtgactgga ttagcttcgt gtacagtttc 8580

cttcgtgtac agtttccctg tccactcttc tgagaggaaa tgcagaggtg gtggaagggg 8640

tctggggtag ctgaatgcat gtgagggaag tcggtccctg agtgaaggag aaagggagga 8700

ggactgggtg gaactttcct aaacttctgt gttgttctat gaaggtccag caaggtcact 8760

gaatcagagt cgtgtcacag tttcccatcg gggacccagt gactcccagc agtggctctg 8820

ctcagatcag cgcagagctt gttcatctcc taagagtgga gcacaggacg tggctccagc 8880

accagccatg gcatggaaga tagagagcag ccctgggtgc ctgggtcagg tgcattgtgc 8940

tccctgcagg tggagggagg gaagtgctga ctcagggccc agagactgtg ggttctatac 9000

agaacataca cttttacttc atttctgtgg atgacataga aacaaacatg cagtctgtaa 9060

acaatggtga ttcctacatt tgccccagtt gctttattcc ttgattctgc agaatgtcct 9120

ggcacagaat tgcctttctc atgtgaaatt gtgatgtgtg ttcaggatgt gtgatcccta 9180

ctttcattgt tttcctccat gtacagagct cctaaataga gtacagctga ccctccttct 9240

acactgttcc tctcctcccc acaggaagag cagataatta cctgaggctg aatctgaggt 9300

gggatctgtc ctctgaacct cagagcctgc agagaccccc agctgcagat tcatggagtc 9360

aggtgtttgt acatgtggga accttgagct gttcttttgt cagtgaacac tccttagaac 9420

taattgtggg ttcagaatta ggacacccat tgatctatca cacctcagct cattctgcca 9480

ctcagaatct ccagaaattc aggaaatggt tgaatgtaca tttttgtgac aaatttttct 9540

catttcactt agttgtgagt tttgttagca gaaagtgcta ccatttatgg gtcccaaact 9600

gatgaagctc atttgcttca acagaagtta ggaggctcct aaaacttctc atcatcccct 9660

ctctctgcac ttcatgtgaa attcagtttt acctggaatt ctggtgtgtt ggtttagcca 9720

gatttcccca tcctccatgt ctgattttcc tgggtatctg atcagtttcc ttgcccttca 9780

ccatacctcg gtgacgtcta atcaccctgg cccaacatca agaattctgg caggttggtt 9840

taagcaggat ttgctgtgcc cctgatgttt cctctcagta attttccatc ttccggaccc 9900

caccctgctg cttggcttta aatccccaca tttcctttct gcatttggag ttcagctgaa 9960

tctctctcct cactgcaaaa caccattgcc ctgatcccaa cacctaccat gaggaccctg 10020

gataaagtct tccttactgt gctggaacaa gtgtctttac ttgatatttt ttataatgct 10080

gcctgaaagt ctctcgaaca taacttttta ttatgtcgag atgtgttctt tctataccca 10140

ttttttacat tttttttcct gaaaggatgt tgagtttcat caaatgattt ttcagcatca 10200

attgaaaaaa actatatgtc gattaaagat ctaaatgtaa aatctaaaac tataaaaact 10260

ctggataata acctaggaaa tacaatttag gacattagaa ctggcaaaga cattatgaag 10320

aaaatgcaat tttaacaaaa tcaaaaaatg gcaaatgagc cctaagtaaa ctaaagagct 10380

tctgcatagc aaaagatact atcaacagag caaacaggca acctaaagaa tggaaaataa 10440

aatatttgca atctatacat ctgacaaggt ctaatattta tagcatacat gcaattaaac 10500

aaacatacaa gaaaaaaagt gcccaaagga catgaaaaga cacttataaa aagacctaca 10560

tgtggccaac aagcatagga aaaaatgctg actatcacta tcattagaga aatacatatt 10620

aaaaccacaa tgaggtacca tctcacatca gtcagaatgg ctaatcttaa aaaaataata 10680

acagatgctg ctaaggttgc aggaaaaagg gaacatttat acacttctag tgcaagtata 10740

aattagttca accattgtgg aaagcagtgc catgatccct ccaataacct aaaacagaag 10800

tttcatttga cccaacaatc ctacaactgg acatatacct aaaggagtat aaacgtatag 10860

gttcattcca gcactattca caatagcata gacatggaat ttacctaaat gcctgtcact 10920

ggcataatgg atagagaaaa atgtgataca tataaccatg gaatactatg cagctaagaa 10980

aagaatgaaa tcatgttctt tgtaggaata tgatggaact ggtagtcatt actcttagaa 11040

aactaattca ggaacagaaa accagatact atatgttctc acttattttt tggagataaa 11100

taatgagaaa tattctctca gggcctgagt cttccatatt caaaaaaaaa ttctaaaata 11160

agtgttcaac aagttgccga catgccttta aatatcctgt ttcatctgga ccccttatat 11220

cactcaataa agccatgaga actatattta aaagtgggat tctatgataa taaataactt 11280

aacagtaaga ataggaagga tatggatggt tttactaatt taatgggtac ataactgtgg 11340

tatacactat attcctatga ataagatatt ctgatttcag agctaagtat tgtttcctac 11400

gttgtgtgtg tgacttggtt gtgttggatt tgagagtgta gcacgtgcac agaccatgtg 11460

gatcagaaat ccacatgtaa agatgacaat ctatggatgt gaactacaag tatgtaagta 11520

cttcaagtaa ttctatttaa tggagtttga aataaaactc aaacttattc aaaacactaa 11580

ttacttggta tttattttga gatctatgag tttatcaaga aattcaaatt cctatttcta 11640

tttaaactcc cgattcctac tctcagtggg agggaaaact cacagccaat cacacatcac 11700

aggacaaatc tgtaaacgaa gagtcattcc tctgaaggtc ctgggtgttc aggactctca 11760

ggcaggtgct gaggaccctg tcttgggagt gcccagcaga tctcagaacc ctacatgggg 11820

cctgctggac actcatgtgg gataactagt cgccacttat tcagagttac cagtgagctt 11880

tgactgttcc gaatgggacc agcatggagt caaggtgcct gctcaatgtc agagacagcg 11940

atggtctcag aaacaatcca ggtaatctct aggccaataa aatgtggatt cacagtgaga 12000

agtacatcct ggaggtggag cttgttcttc agtgggaaga gtgctgtgca cagaaagctt 12060

agaaatgggg aagggggtgc gtttcctcag gcaggattag ggcttcgtcc ctcagcgtcc 12120

cactcttgta tggctgatgt ggcatctgtg ttttctttct catactagat aaggctttga 12180

gctgtgaaat accctgcctc atgaatatgc aaataacctg agctcttctg aggtaaatat 12240

aggtatattg gtgccctgag agcatcactc aacaaccaca tctgtcctct agagaaaacc 12300

ctgtgagcac agctcctcac catggactgg acctggagga tcctcttctt ggtggcagca 12360

gctacaagta aggggcttcc tagtctcaaa gctgaggaac ggatcctggt tcagtcaaag 12420

aggattttat tctctcctgt gttctctcca caggtgccca ctcccaggtg cagctggtgc 12480

agtctggggc tgaggtgaag aagcctgggg cctcagtgaa ggtctcctgc aaggcttctg 12540

gatacacctt caccagttat gatatcaact gggtgcgaca ggccactgga caagggcttg 12600

agtggatggg atggatgaac cctaacagtg gtaacacagg ctatgcacag aagttccagg 12660

gcagagtcac catgaccagg aacacctcca taagcacagc ctacatggag ctgagcagcc 12720

tgagatctga ggacacggcc gtgtattact gtgcgagagg cacagtgtga aaaaccacat 12780

cctcagagag tcagaaaccc taggggagaa ggcagctgtg ctgggctgag gagatgacag 12840

gggttatcag gtttaaggct tttttgaaaa tgggttatat atttgagaaa aaaataacaa 12900

tagaaacaag tacacactct aattttaaga gatatattca attcaagaat tgtagaagcc 12960

gaattcacag tgggaaaggc cacactcaat aaagttgata aaaacattcc aggaaggtgc 13020

tactgtgaac aagttttcaa attagatgaa taaataattt ggagcaatgt tatttgatca 13080

tgaggtgaga cagtatgatt cttaaaaagt ggaaaaaatt ctttcaaatg tttctgccac 13140

tctttatcat aaaattaatt ttagagcagt tttaggatta caatgaaatt gcacagaagg 13200

catgagaatt cccatgactc cctgccctac acaggcagag cctcctccac tatgaccatc 13260

cagcaccaca gtcacaaatc agttacaatg gaggaatctc caaggacatt tgattctttc 13320

ttttttggtg atgccctaat ataacaagcc taatttatct cggaatacca caagttttgt 13380

tcagtggatt tctaggaatc atattagtta gagggaaagt tggtgaggct cttactattt 13440

gaactcattc ttccaaaatc cacaaaacat atattaattt aaagcttatc ttacttctgg 13500

tttacaaaga tccttcccag agagtaagat ttttaagatt ttagaggcag gttcatgtct 13560

ctaaaaggcc agcaagttct ggggaatccc ataatgaaca tcctttcatg ggaattggag 13620

accctggcaa tgagagactc catgtataat gccctagagt tggattagat gcactgtgag 13680

ctcttgggtg gtggttctga aaaaggtggt ctgtgcagta aatgcaaaca catgcacggg 13740

acacaggaag gagcaaaagc ttccctttac aaagtgtgtg ggcacctgga agaaaccctc 13800

acaagggcac gtctcaccct taatgactgc acattagcca ggggcatgat catgattggt 13860

cttacaagga aagagcacca ctgaggtcat aggttatgaa aatgtcatcc tccagctgag 13920

aaattccatc tgctggcatg tgcgtgtcaa ccccgtggag ggtgcactct gggagttgac 13980

gagatgcata gaaacctcct gtcatgaatt atcgtctacc acacagtcag aatcaacctg 14040

tgctccagaa agagatacat gcctgtagaa ataaacagag cttaaggatt ttcttacctg 14100

ctgaatatag tgcccaaaac cacactttca ggaagattag ctcagaaatg tttacaaagt 14160

gactgaaggg cagtggcggg tgaggtgatg ggacagcctc agggctgcac ttgaggaggg 14220

ctccctctcc catgcaggct tttcctccag gagctgcatc aggaactcac agaggataag 14280

ggagaattct gagaacatgc tactgtggta ctgcctagag aggaaggata aatgatgaca 14340

aatacatctc tgagtaacat atgggcattt gttcatgaaa actgtttttt ctgaaagctt 14400

atgaaggtct tgaaatgccc ctgattagct gaggacaaac atttaaaccc tccttccaca 14460

gggagttcaa gcaggctgga tgtgtcctgc tatggatgat cttccccagc cccttcctct 14520

tcccagctca tccctggctc tctgtgtaaa aagttttcat cagtggaagg tggttgatga 14580

agtgaggtct tcaatttcct catcttctct gtggtcatgt tattttcctc atctgaggtt 14640

taaaaactca cctgcatgca gcacatgaca ggctaaaatc tcttgtggac aaaacagtaa 14700

caaaggcacc caccatggtt gagcacccgt gttgctgaca gtgaccacca agggtcaacg 14760

tcctcttcac aatcctgtgt cagagcagca cttgagtgat ttcactagca acttcccagg 14820

agaatcggct taaaactact tgtcgcattt tccatacaga tataacacat ctattttcct 14880

gaagaaatag aaagagctga atgctgaata cactgtatgt ctgctggttc tgcaagtttg 14940

tgactatcac tttctaattt ctgatctgtg tggaccaccc tcactggtgt gaccagccgg 15000

gtgtgcccaa agcttactca atgtttatac tgcattgctt ttgatttctt tatatatttg 15060

tcccctccaa atctcatgtt g 15081

<210> 11

<211> 14191

<212> DNA

<213> artificial sequence

<400> 11

tcactggtgt gaccagccgg gtgtgcccaa agcttactca atgtttatac tgcattgctt 60

ttgatttctt tatatatttg tcccctccaa atctcatgtt gtaatatgat tcccagtgtt 120

ggaagtgggg ccactaggag gtgattagat caagggggca gatccctcat gaatgattga 180

gcatcttccc cttggtgatg agtgaattct cccccagtca gctcacacac agtctagttg 240

tttaagtctg ggccccccct cagcctcttg ctcccattct tgccatgtgg catacctgct 300

ctcccttcac cttctgctat gattgtaagt ttcctgaggt cctcgccaga agcagatgct 360

gatgccatgc ttcctataaa gcctgcagaa ctgtgagcta attcaatctc tttcctttat 420

aagttaccca gcctcatgta tttctttaca gtaatgcaaa gggacaaaca caggtggctc 480

ctgacacacc cctcatcttc tcctttgtca tcatctggct acttccatgt gtgtctgtgt 540

cctctcctct catgaggaca ccagtctttg gatttgggac ccatcgaaaa tttagtatga 600

tatcatctca agtccttaaa ctaatttgta aagaccctat ttctaaatat ggtcacattc 660

tgaggttcca ggtgggcata catttggcca gggagatgcc atcaacccag cacaccagcc 720

tacgcaggta catttgcata tagcttaggg ttgacctttc ccatggaagt attacatccc 780

cctctggact ccagtttcac atgttttaga atgcttcgcc ttgtccagca ggatgagtct 840

cttttcattt ttagtatttt tctctctctt ctttgcatta gattaaaaat aattgacata 900

gtctattgat gcatctttaa gttttcagtt ccttctcatg tctgctccat tctgttatga 960

aacttgagta atgtttttct atttcttttc ttttttaata aaatagagat gggatttctc 1020

catgttgccc aggctggtct caaactcctg ggatcaagtg atccttcctt cttggcctcc 1080

caaagtgcta ggataacagg catgggccac tgtgcctggc caaatgatgt ttttcatttc 1140

agaatcatac ttttagttct agaattttca tttggttctt gttaatattt tccctttctt 1200

acagagattc cccatctagt cactcattat aaccatattg tcctttaagt ctttgaacat 1260

atttaacatg acttctttaa agtccttgtg tgaaaactgg gtctgcatct aggtcatctt 1320

ggagttgatc tccattgatc cctttttctt ctgactatgg atcacatttt catgtttctt 1380

tgcatatatg gtaattttgg atgtgcacct gatgttgttg ataacatgtg gtacaggcta 1440

tgggttttgc gttcttcctt ggcagggcat tgattgtttt tttcaatgtt aggaaacagc 1500

ttgagttgac tcaaactccc aagtctgtct gcctggcagt tggcagtagc tggaatctca 1560

gttctctcga ccttacaggt gctgctttct gctgggccct ttggagtttt ccctacccat 1620

gcacacctaa gggatcggcc agaggtttca gtggagttta cttgcagatt gtggggtttc 1680

cctttggtga ccttctcctt tatggacatc ttctcttcat ttccagctgc tctgaaaatg 1740

cagccctgca tcccactcct caccaggagg gctgcagttt cctgcttgaa ctctagctgc 1800

acccattaca tgccctgggg tgtgacttca gaccaatatt tccgggaata atccttacta 1860

gtgtttgcct acttctggtc atgttccagt gcctgcaatt ggtgtgtgtg ggtgttgtgt 1920

gtgcttacag cttttccagt gtttataatt gccatctgcc aaagggctag tctgatatta 1980

gctgctccag cattactgga atcagaacta ctttctctca tgtggttttt cattttcatt 2040

tccctgttga ctagtgtggt tcaacacctt ttcatatgtt tagcggctat ttggatatct 2100

tctgtaaaac atctgttcaa ttcttttgcc tattccttgt tgaattattt gatttttttt 2160

ctcgttggtt tacaggggtc ttctttatat tatggatctg tttgtgtcag tcagttatat 2220

atgtttatag gaaacattga gaaaaacaaa agattagtag gtgccttcca tggaaagcaa 2280

ggcatcgtct tgtaccctct tccaggttat ttcattctat tgagctctgc atcttttatt 2340

tcctttgacc tattttacac ctctataagc caggggttcc cttcatcaca ccatatcaca 2400

ctatgtcaca ccagggatgt cacaccacat cacactatgt aactcctcat cacactgggg 2460

atgtcgtacc aggtcacacc ccatcatgcc acatcacact gtgtcacacc gcatcacatc 2520

acaccaagta tgtgacaccc tgacacagca catcagacat cacatcatgt cacaccacac 2580

ttcatcacac cccaccccac cagagttgtc acaccccatc acaccacatc acattacgtc 2640

acgccatgtc aaactacatc ataccccatt accccaggga tgttatgcca catcacatta 2700

tgtcacacca caatacacca catcaaattg tgtcacacca catcacaccc catcacacca 2760

gggatgttat gccacatcac cttatgtcat gccacatcac accacatcac accccatcac 2820

accgtggatg tcacccgtca tcacaccact tcacataatg ccacacaaca tcacacccca 2880

tcacaccagg ggcgtcacgc cccatcagaa cacgtcacat tatgccaaac cacatcacat 2940

catgtcacac cacaccacac cacactatgt cacagcctgt tacacttcat cacaccacat 3000

cacactaggg atgtcacacc ttgtcccatt acatcacatc atgtcacacc attccacatc 3060

aaaccatatt atgccccatc ataccaggga tgtcatacca tataatacta tgtcacactg 3120

catcacacca gggatgtaac accacatcac accatgtcca accttgtagc ccaacaccac 3180

atcacagaag ggacattaca ccatgtcata ccacatcaca ccatgcaact cctcatcaca 3240

tgagggaggt cgaaccctgt cacaccccat catgccacat cacaccatgt cacactgcat 3300

tacatcacac caacgatgtg acactctgtc acaccacatc acacatgtca caccagagat 3360

gtcacactcc atcacatcac atcacatcac acccgttaaa tgatatcata ccccatcaca 3420

ccagggatgt cacaccgcat cacatcaggt catatcacat cacactacgt cacaccacat 3480

cacaccaggg gtgtcacact ctgtcacagg atgtcccacc atgtcacacc ataccacatc 3540

acaccatatc atatcctatc atactaggga tgtcatgccc catcccacta tgtcacacca 3600

catcacacca tgtccaacca tgtcacatca catcacacca catcacacca tgtcacacca 3660

gggatgtcac actctgtcat accacatcac accatgtcct gtcacatcac acattacacc 3720

ctggcacacc atggatatca caccacatca caccatgtca tgttacatca cagcatggac 3780

tgctaggggg tgcgtggagg cagccttgct ggagagttga gggagggtcc tggggctggg 3840

cgtggtgttc ctgcaggagg actggccctc tggaggatgc tcggttccag gtggaaaggg 3900

ggaggtgggg cctgggtgct tcagggaggg gccatccttg ggcttgggat agagccggtg 3960

atgcagctgc tgcccgccct gcaccccagg tgctctctcc ctcacccccc gcggggctgc 4020

agcagcgtgt cctgagagtt aaagggctgg gcttcagcac ccagttcagg ccaggcaccc 4080

tgaagcccac cctccagcgg cgagccctcc cacggcacag cagggtccgg gctctgggga 4140

tttcaccccc aactctggtg cagctccagc tgctcgatgc cacacaaacg aatccaacca 4200

ctcctccttc ctgggtgaga tggtctctct cctgccacag gcaactccga cggcattttc 4260

cagccaccgc agccaccgca gccactgcag taacaagacc ctgtccttga ctgagttcca 4320

gccaggctcc tcggagcctc tccactcggc ctcaaccttg gcttgtaaag acttgagcag 4380

acactaacag tttctaacag cttctggccg tacccctagg ccgacccctg ccccgtcaac 4440

acctgcctga gaaagctccg tgcaccagaa ctcaccgttt ggaccaaccc cgacctccct 4500

ttctcagggt atctgctgag agggccgcaa ccacacgacc ttctatccgt tcccgatgtc 4560

tgtgcatttc ctgtgaccca ggagggtctt tctcaagact tgagagccgc tccctgaagt 4620

gtccccattg ggaaggatgg ggcctgtgtc tccaggctct gggaggacag aatcctgacc 4680

tcaacagtgg ccggcacgga cacagcgggt cccatcccgg ggacgctgac cagcgctggg 4740

caacttttcc cttccccgaa gactgagccc cgagcaccct ccctgctccc cctaccacct 4800

ccctttacaa ggctgtggcc tctgcacaga tgaaggtgag tccaggtcat gccggactct 4860

ttcttctgtt gcaatagtta tttctgttga aaatctgtcc ttgctacatg acctagtgcc 4920

cagggggatg ctgagacagg atgaatgtat tctgcatgtg agaagaacat gaattttggg 4980

gcccagagtc tggactgtga tgggttaaat tgtggcccct acaaattcat atattcaagt 5040

cttaatccct ggcctcacaa tgtgactatt tggagatggg gtctttacag aggtcattaa 5100

gttcataggg ggtcactaat ctaatccgat gtgtgttcta agaagagaag cttaggacac 5160

gggcacacag agggatggcc acgtgaggac cagggaggag acggtgtcta caagccaagg 5220

agagagggct tgagagaaac cagccctgcc tgcatcctga tctcagattc ctggtctcta 5280

ggcctgggag gatccatgtc tgccgtggga gccgccccgc tgtggtcctg agctgactca 5340

cacagatctg acacccacct ctcgcttcgg accatggttg gttctggaag gccctccctg 5400

tggctctgcc tggccagcct gagccagctc ccagcctcga cccagctttc cctggaggcc 5460

ctgtcccccg cagagtgacc agggcaggca gcaccgtgcc cagcaggagg agaaactgca 5520

tccatgtaga aaagaggaga agccccgggg gtccatgtag cgacaggggc cagggagggt 5580

cgctcgggca atgcgtgtgg ctgcaggagg cggggggcgt gtgcagggag cccccgaggt 5640

gcagctggac cagcctcctc ctgaccgtgt ttcccaccgg gggcaggagg cacgtggaca 5700

caggaaggcg gctcccatca cgaagtacaa gacttaaaaa ggatatttta ttgtcatcac 5760

aaaagaaaca tcaaagacaa ttaatgaact ttagaaaatt taaaagaaga aaagctacca 5820

aagctgaaat ggtggcacct ccttcgagtg agcccgggag tcctccctga cggctgaggc 5880

aggcgctggc cgcactcccg ctcgagtctc ccttcctgtc tgcggattct gcgtgacagt 5940

cacggaacgg cgtgatgggg gcagcagagc gtgggggcct ctgtccagca ctcgtggcca 6000

gcagccctgc tttcgcaaga acacgggcac cctctttggc gtcttgcctc tccacctggt 6060

gcccccagag tggctgcttg ttcctgctgc acgtgacccg ggactggacg ccagcctctg 6120

tgatgagttc tggctgtgtc cacgctcctg gctctcccgg tgtccctcca cctctctccc 6180

cgatgctcct gggcctcctc tgtcctcagg ccccaccaag gctgagtctt gcccgcctgg 6240

gacctggtca ccagccttct ctgggaggcc tgtctgggca gatgcccagc ccttccttgg 6300

gctatcctca cccttgcact gtggggctcc tgcagcggcc acatggccca ggctcttctc 6360

tgagtgatct cggtggactg gagtgggtgg gaggtggcag tgtcctgggc ctggcccctt 6420

ctctccccag tgcggactct ggggctggct gtccctgcgg gtcgagttcc acccgagaat 6480

ccagcagtgt gggcaggcag ccaaggggtg gtgctggcac cgagactgtt ctcaggagcc 6540

agagagcagc gttctttgct tgaaatcaga acaacctcat tcctcatgtc aggagttcac 6600

gggagtgccc ggaatggagg ctggctggct gcgggctggg aggaaggccg tctgagtgag 6660

ccttcgcagc tcttggaagc ctccccaaca gggcctgatg gtgctgtggc ttccctacct 6720

tggcggctga ttttcccact caccaactgg aaaccacgcc tgtgttcagg aggctggtgt 6780

ggacggggtt ggctccaggg cgaggtcctg cctgggtggg ggcctgggat gccggtcact 6840

gactcctttt gtgtgagcac ctggtggtct ggagggcagg gacgtcctgc tgaggggaca 6900

cctggccccc agcgccctgc atgcatcaag cagcggaggt ctggggtaga cctgctatgc 6960

acagggtctg gaaggggggc gtgtcagggg tcagaaggtg acttcgaggc cagagagcca 7020

tggggttcag ggcggtgagg tcgggggcag gtgtggcctg ggtggtggct gagcatggcc 7080

cacggctcgt gtgtggggtc tgggcggccc tggacacccc gcagagggtg gccctaggcc 7140

ccctgcccga tcatgttcct gtagtcgggg acgatggtct gcttcaggtc caccaccgag 7200

gagaagatcc acttcacctg taggcaaggc acagcacagg ggtgagcgag gccacagccc 7260

tgtccccgag ccccacccac ccctcagggc actgagggcc acatctcttc ccccaaggtc 7320

cacccacccc tcatgccacc caggccacag ccctgcccct gaggcccatc cgcccttcag 7380

tccacccagg tgccagggcc tcaccactgc ctgctctgag gcctggacat ggagagcaga 7440

gccagggcac caacagcatg tgggcagtac agaagacagc gtcagggaca ggtggagaca 7500

gtgtggggga tagtgttggg gacaggtggg gacagagtgg gggacagtgt cagggacagg 7560

aggagacaga gtggtggaca gtgttgggga caggaggaaa cagtgtgggg gacatttttg 7620

gggacaggag gggacagtgt gggggacagt gttggggaca ggtggggaaa gcatggtgta 7680

cagtgttggg gactggtggg gacagcgtgg gggaaagtgt tggggacagg aggggacagc 7740

gtgggggaca gtgttaggga gaggtgggaa cagtgtaggg gacagtgtca gggggaggtg 7800

gggacagcgt gggggacagt gtcagggaaa ggtggggaca gtgtggggga cagtgtcagg 7860

gagaggggac agtgtggggg acagcgtcag ggagaggtgg ggacagcatg ggggacagtg 7920

tcagggagag gtgcggacag catggacagt gtcgggacag gtggggacag tgtgaggaca 7980

ttgttgggac aggtggggac agtgtggggg acagtgtcgg ggacaggtgg gaacagcgtg 8040

ggggacagtg tcagggacag gtggggacag cattggggag agtgtcaggg acagatgggg 8100

acagtgtggg ggacagcatc agggacaggt ggggacagca tgggggacag tgtcaaggac 8160

aggtggggac agcatggggt acagtgtcgg agatgggtgg ggacagcatg agagacagtg 8220

tcggggacag tgtcagggac aggtggggac agcatgtggg acagtgggac aggtggggac 8280

agcatggggg acagtgtcag ggacaggagg agacagcatg ggggacagtg ttgcatacag 8340

gaggggaaag catggggaca gtgtcaggga ctgtagggga cagagtgggg gacagtgtca 8400

gagacaggag gagacagcat ggggacagtg ttggggacag gaggggacag cgtgggggac 8460

agtgtcgggg acaggtgggg acagtatggg ggacagtgtc ggggacaggt ggtgacagcg 8520

tgggggacag tgtcagggac aggaggagac aggagaagac agcatggggg acagtgtgag 8580

gtacaggagg aaactgtggg ggacattgtt ggggacagga ggggacagcg tgggggacag 8640

tgtcagggat aggaggagac gagaagacag tgtaggggac agtgtcggga caggagggga 8700

caggaggaaa cagcatgggg gacattcagg gacaggaggg gactgtgggg gacagtgttg 8760

gggacaggtg gggatagcat ggggtacagt gttggggact ggtggggaca gtgtggggaa 8820

tagtgtccgg gacaggtggg gatagtgtgg gggacagcgt cagggacagg tggggatagt 8880

gtgggggaca gcgtcaggga caggtgggga tagtgtgggg gacactgtca gtgacagttt 8940

gtgacagcac aggggacagt gtcagggaca gggaacgtgt gggggacagt gtcagggaca 9000

gtttgtgaca gtgtggggga cagtgtcagg gacaggtggg tgcagcattg gggatagtgt 9060

caggcacatg tggagacagt ctgggggaca ctgttggaca ggtgggtaca gcgttgggga 9120

gtgtcaggga caggtggcga cagcgttggg gatagtgtca gggacatgtg gagacagtct 9180

gggggacact gttggacagg tgggtacaga gtgagggaca gtttgtgaga gcgtggggga 9240

cagcgtcagg gacaggtggg gacagcctgg ggacagtgtc agggacagtg tgtgacagca 9300

tgggggcaat gtcaaggaca gctggggaca acgtgcggcc gaccttgaag aaggtgacgg 9360

tggcactgta gcacacgctt agcaggaaga gtgtgatgaa gatggtgatg gtcgtccaca 9420

gcccgtccag ctccccgtcc tgcgcctccg cacagctctc ctccagttgc agctctggac 9480

aggaaggggg tggtcagtgc tgtgtccccc tgggcttggg cctctggggg tgattccctc 9540

tgtggcgggg cctaggatgt agggcccggc ctcgatggcc caacagtgtc ctgaggtcag 9600

ctcccggaag ctgtccatcc tgggcaccgg ctttggccct ggggctcagc cagacacccg 9660

gccctaaata gcgacctggc cctcagcagg acccgctccc cgtctcccgt gtccctccct 9720

gagccccaga gggcaggaga tatgaagccc acccctcatg tgaccccagg agcagggaag 9780

ggctgtattg ggaagtgggc cagggccagg gacgtgacgt ggtgtgtgat cccctgtgtg 9840

tgtgtggcgg ctgcaggggc actttgtgag aggaggactg ggtttgtctg agctggtcag 9900

caagtggaga agctgccgag aggctcgtgg gccttgaggt gccgcatggg gcttgtaggg 9960

gcctgtgtcc gaggagtgtt cacgtgtgcg aggaccttgc tctggtctgg gtgctgtgca 10020

gttcgcccgg gtgaggctcc gtgtgtgagg cgtgcacgtg tgtgtgtggt ggccgtgtgg 10080

ccggccaacc tcagtgcggg gtttgttgaa cgggtctggg ctgagtgtgt gtgtgggcat 10140

ctggaccagt ccctccatag ggcccgagag tgcatgtccc cggagtcggt tgtgtcccca 10200

tgcgggtgcg aggctgggca gggctgccag gggttagtgc cgtgggggta gatgggtgag 10260

ggagggcctg tccctacgca catggactag gcatgccccc gagtgggcat ggggggtcgg 10320

aggacagggc gctcacagaa caggacagtc tcctacagag gcaggggctg tgtgtctgtc 10380

cccaggggct cctagggctt ctcgtggctc agcccagggc aggtgctgct ggagggaggg 10440

ccacgctggc aaatccccca ccctgccgag ggcagcccct ggctgagccc caccctaggc 10500

ggcccaggca cacctgcaca gcctgggcca gtgtggggac agtgggaccc gctctgcctc 10560

cctcatgcca ctcaggcctc agactcggcc tgacccgtgg aaagaaccat cacagtctcg 10620

caggggccca gggcagtggt gggtgcttta tttccatgct gggtgcctgg gaagtatgta 10680

gacggggtac gtgccaagca tcctcgtgcg accgcgagag cccggggagc gggggcttgc 10740

cggccgtcgc actcatttac cccggggaca gggagaggct cttctgcgtg tagtggttct 10800

gcagaccctc atgcatcacg gagcatgaga agacgttccc ctgctgccac ctgctcttgt 10860

ccacggtgag cttgctatag aggaagaagg agccgttgga gtccagcatg ggaggcgtgg 10920

tcttgtagtt gttctccggc tgcccattgc tctcccactc cacggcgatg tcgctggggt 10980

agaagccttt gaccaggcag gtcagggtga cctggttctt ggtcatcttc tgggatgggg 11040

gcagggtgta cacctgtggt tctcggggct gccctgtagg gacagaggtt ggtacagcgg 11100

tcactcccag ggcagagggt gggccaagcc ggcctctgtc cacgtggcct tcgcgctccg 11160

tgggtcccac ctttggtttt ggagatggtt ttctcgatgg gggctgggag gcctttgttg 11220

gagaccttgc acttgtactc cttgccgttc agccagttct ggtgcacgac ggtgaggacg 11280

ctgaccacat ggtacgtgct gttgtactgc tcctcccacg gctttgtctt ggcattatgc 11340

acctccacgc cgtccacgta ccagttgaac ttgacctcag ggtcttcgtg gctcacgtcc 11400

accaccacgc acgtgacctc aggggtccgg gagatcatga gggtatcctt gggttttggg 11460

gggaagagga agactgacgg tccccccagg ggttcagttg ctgaggaaga gatggaggcg 11520

gacgtgtcag cacccggttg gggcctgtcc ctggacgcag gctactctag ggcacctgtc 11580

ccgccttgag ctggagggcg aggcctgggc tggcttactt gcacatggtg ggcatgtgtg 11640

agttgtgtca caacatgggg ttttgggctc tgcagagaga agattgggag ttactcagat 11700

ctgggaggag aggtgtctga gctgagggag tggagatctt ggcctttggg gtgggcttag 11760

gtcaggggca gggtcttccc ggatatggct cttggccagt ctaagtgcag cacctgcccc 11820

tttgtgcgca gggcctgggg taggggcttc cagcctgtgg ctgcctggag cctggtggaa 11880

aaagccagaa gaccctctcc ctgagcatga gtggggcggg cagaggcctc cgggtgagga 11940

gacagatggg gcctgccttg ctgccctgga ctggggctgc acagccgggg tacgtccagg 12000

caagagggct gagcctggct tccagcagac accctccctc cctgtgctgg cctctcacca 12060

actgtcttgt ccaccttggt gttgctgggc ttgtgatcta cgttgcaggt gtaggtctgg 12120

gtgcccaagc tgctggaggg cacggtcacc acgctgctga gggagtagag tcctgaggac 12180

tgtaggacag ccgggaaggt gtgcacgctt ctggtcaggg cccctgagtt ccacgacacc 12240

gtcaccggtt cggggaagta gtccttgacc aggcagccca gggccgctgt gccctcagag 12300

acgctcctgg aggagggcac cagggggaag accgatgggc ccttggtgga ggctgcaaga 12360

gaggtggtgc catgtgaccg cggtgtggga cagagctggg cccagggtgc agaggcccct 12420

cggttcttgt ctatccgcga gggtccaggc agggtccagt gtctgggctc acgggcattg 12480

agtgtgcacc tggctggtgc cacctgcctc accttagccc cctccctgcc ccaaagccaa 12540

agtcaggccc ggcctgcccc agaaagcttg caggactggt ggccctgtgg tgcccttctg 12600

caggcacccc tgcagcctag ggggcggggc tcggcagcca ggtcagtgct ttgtctcaaa 12660

aaaaacaaaa acaaaaacaa aaaacaaaac aaaacaaaaa acaaacaaat aaaaagttgt 12720

aaaaggattg tggaaaaggg atcttatgtg gtcaaaggcg gctgtgattg gatttattta 12780

tttatttttt tgagacagag tttcactctt gttgcctaag ctggagtgca atggtgtgat 12840

ctcggttcac tgcaacctct gcctcttggg ttcaagcgat tctcctgcct cagcctccag 12900

agtagctggg attacaggtg cccaccacca cgcccagcta atttttatag ttttagtaga 12960

gacaggggtt tcaccacgtt ggccaggctg gtctcgaact cctgacctca tgatccaccc 13020

gcctaggcct cccaaagtgt tgggattaca ggcatgagcc actgcacctg gccggattta 13080

tttatttatt tatttttgag acagggtccc actgtgttgc ctaggcagca gtgcagtggc 13140

actcagcact gaggctgagg gaggttgagg tgggagggct caagcaatcc tcccacctca 13200

gcctcccaag tagctgggac tacgggcacg tgccaccatg cctggccaat tttttttttg 13260

tattttttgt agagacaggg cttccccatg ttgcccaggc tgatctcaaa ctcctgggct 13320

taagtgatcc accctcctgg aatgctggga ctacaggtgt aagccacctt gcccagcctg 13380

gatggaatta tttataaggt ttaattaaaa ttagctttaa tattaacagt tcatgtgaaa 13440

ctagaatttg gtcttctctg ttaaagtgac agttttctgg aatattggtc tgctcttcat 13500

tatggcaggt ttttcttttt tttttttcac cttgaaaaat atatatttac aggaacaatt 13560

ccccattctc tgggactctt tagaaaaaaa aaaggtccat tttggggaag caaaacagtg 13620

gagacgagtg tagcaccgtc ccccaaatca ccaaccccca ggtcccaagg cctgggctgg 13680

gccagggctg acagggaagc ccaggagtct tttgaaccca ctcttcctgc ctagaataga 13740

gacaggacag gctttatgtc ccccattcct ccctcccacc tccagggaca ttgaaagtgt 13800

cctttgtacc tacctgtagg aaattggggg gctgggaggg agggaactga aaatacacat 13860

ttgttatcaa aaataaacat ctggggggga ggcggcagga agattccctc ccaaatccct 13920

ttcttcacac ccaccccacc aaatatagga agagatgact ccctctcccc tattgaaaag 13980

ccccatttaa aaatagatta tactatcaaa atggcagcac gggagagaca gggagacctg 14040

gagtactggc tggaggggcc cccccagaca ggaaccaccc ccacaaaacc cctccatggg 14100

aggaaacagg caggacccca gggagtttgg cagacaaagg aatggcttct cagggggaag 14160

aagaacaaag ggtaatcatg gtcatagctg t 14191

Claims

1. A method for improving the conversion efficiency of saccharomyces cerevisiae DNA fragments is characterized by comprising the following steps:

(1) Synthesizing target DNA fragment: synthesizing the DNA fragment of 5-20 kb;

(2) Performing methylation modification on the DNA fragment in the step (1);

(3) The DNA fragment subjected to methylation modification and the linearized plasmid are transformed into saccharomyces cerevisiae by using a PEG-LiAc method;

the methylation modification is obtained by methyltransferase treatment, and the methyltransferase is formed by combining CpG methyltransferase, gpC methyltransferase, taq I methyltransferase and DpnM methyltransferase according to the enzyme activity ratio of 4:3:1:2;

the 50. Mu.L reaction system for methylation modification is as follows: adding 2-5 mug DNA fragment and 1-10U methyltransferase, adding S-adenosylmethionine with final concentration of 80-160 mu M, 5 mu L10 Xreaction buffer, ddH ₂ O is added to 50 mu L, and the mixture is reacted in a water bath at 37-65 ℃ for 1-5h;

the 10 x reaction buffer composition is: 200mM Tris-Ac, pH7.9, 500mM KAC,100mM MgAc2,100mM DTT, 1000. Mu.g/ml albumin, the balance being water;

the DNA fragment is designed with a homology arm for connecting plasmids;

the plasmid is pRS415.

2. A method of improving the conversion efficiency of a saccharomyces cerevisiae DNA fragment according to claim 1 wherein said DNA fragment is a linearized DNA, or a circularised plasmid DNA.