CN110862915A - Culture dish sealing method - Google Patents
Culture dish sealing method Download PDFInfo
- Publication number
- CN110862915A CN110862915A CN201910991185.XA CN201910991185A CN110862915A CN 110862915 A CN110862915 A CN 110862915A CN 201910991185 A CN201910991185 A CN 201910991185A CN 110862915 A CN110862915 A CN 110862915A
- Authority
- CN
- China
- Prior art keywords
- culture dish
- sealing
- film
- winding film
- winding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000007789 sealing Methods 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 title claims abstract description 11
- 238000004804 winding Methods 0.000 claims abstract description 31
- 239000011521 glass Substances 0.000 claims abstract description 12
- 230000001954 sterilising effect Effects 0.000 claims abstract description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 5
- 238000003825 pressing Methods 0.000 claims abstract description 4
- 229920006302 stretch film Polymers 0.000 claims description 3
- 239000003755 preservative agent Substances 0.000 abstract description 11
- 230000002335 preservative effect Effects 0.000 abstract description 11
- 241001391944 Commicarpus scandens Species 0.000 abstract description 3
- 238000004140 cleaning Methods 0.000 description 4
- 230000007547 defect Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000003670 easy-to-clean Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
Landscapes
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a culture dish sealing method, which is used for sealing a culture dish of 6-12 cm by using a winding film and comprises the following steps: (1) cutting the length direction of a winding shaft of the winding film by using a modified LZ-manual cutting machine, cutting into an annular ring with the width of 3cm, and placing on a super-clean working table for sterilization; (2) and (3) taking a 6-12 cm glass culture dish, sealing the cover opening by using the sterilized winding film in the step (1), closely pressing the winding film at the joint of the bottom of the culture dish and the cover opening during sealing, rotating the culture dish for 1.5-2 weeks, and then slightly pulling the winding film to break the winding film to finish sealing. The invention uses the 'hand stretching and winding film' to replace the traditional sealing film and preservative film, can reduce the limitation of sealing, can seal glass culture dishes and plastic culture dishes of 6cm, 9cm and 12cm, does not need to be cut, is not easy to break and age, saves time and labor and greatly reduces the cost.
Description
Technical Field
The invention relates to a sealing method of a culture dish.
Background
At present, a microorganism laboratory generally uses a culture dish to culture fungi, bacteria, actinomycetes, and the like. At present, some laboratories do not seal culture dishes, and although time and labor are saved, the culture dishes are easy to pollute. Some laboratories use a sealing film imported from the united states (Parafilm M) for sealing, but have the disadvantages of time consuming, difficult cleaning, high cost, etc. Therefore, a sealing film which is time-saving, labor-saving, excellent in quality and low in cost is urgently needed to replace the sealing film.
Currently, most laboratories use an inlet sealing membrane to seal the petri dish (attachable photos). The sealing film is in a reel shape and has the size of 4 inches × 125 feet (namely 10 cm × 3810 cm), the sealing film is cut into strips of 10 cm × 3cm, and the sealing film is stretched to seal the culture dish interface, so that the sealing film is suitable for sealing culture dishes with the diameter of 9cm and below.
The sealing film has the following defects: 1. it takes time. The sealing film needs to be cut before sealing, which takes a lot of time. 2, not easy to clean. The sealing film is easy to age, the microorganism is cultured for more than 15 days, the sealing film is easy to age and break, the sealing film is not easy to tear off during cleaning, the cleaning difficulty is increased, and the cleaning time is prolonged. 3. Has limitations. A culture dish with a diameter of 120mm cannot be sealed. 4. The cost is high. The sealing film is in a reel shape, the size of the sealing film is 4 inches × 125 feet (namely 10 centimeters × 3810 centimeters), the sealing film needs to be cut into strips of 10 centimeters × 3 centimeters when in use, 1270 strips can be cut, and the price of one reel of the sealing film is 150 yuan, so that each strip is 0.1181 yuan, and the cost of each sealed 100 culture dishes is 11.81 yuan.
Sealing a 6-9 cm glass culture dish by using a preservative film, wherein the operation steps are as follows: (a) cutting the common preservative film roll into annular rings with the width of 2-3 cm by using a scalpel, and placing the annular rings on an ultra-clean working table for disinfection and sterilization; (b) and (3) taking a 6-9 cm glass culture dish, sealing the cover opening by using the sterilized common preservative film in the step (a), tightly attaching the preservative film to the cover opening to be sealed at the bottom of the glass culture dish during sealing, rotating the culture dish for 2 circles, and slightly pulling the preservative film to tear off the preservative film to finish sealing [1 ].
The preservative film has the following defects: 1. thinner, easy to break and high in rework rate in use. 2. Disposable plastic petri dishes cannot be sealed. 4. 3, high cost. Taking a commercial preservative film skilful as an example, a roll of 26 yuan, which is 20cm × 30m in specification, is cut into annular rings with the width of 3cm according to requirements, and 7 rolls are used, the total length is about 30m × 7 to 210m, taking a culture dish with the diameter of 9cm as an example, two circles are needed for wrapping one culture dish, so the length is 3.14 × 9 × 2 to 57cm, the preservative film can wrap 368 culture dishes according to the specification, and the cost for sealing 100 culture dishes is 7 yuan.
Disclosure of Invention
The invention aims to provide a culture dish sealing method aiming at the defects in the prior art.
In order to achieve the purpose, the invention adopts the following technical scheme: a method for sealing a culture dish, which utilizes a winding film to seal the culture dish of 6 cm-12 cm, comprises the following steps:
(1) cutting the length direction of a winding shaft of the winding film by using a modified LZ-manual cutting machine, cutting into an annular ring with the width of 3cm, and placing on a super-clean working table for sterilization;
(2) and (3) taking a 6-12 cm glass culture dish, sealing the cover opening by using the sterilized winding film in the step (1), closely pressing the winding film at the joint of the bottom of the culture dish and the cover opening during sealing, rotating the culture dish for 1.5-2 weeks, and then slightly pulling the winding film to break the winding film to finish sealing.
Further, the culture dish is a glass or plastic culture dish.
Further, the winding film is a PE stretch film.
The invention has the beneficial effects that: the invention uses the 'hand stretching and winding film' to replace the traditional sealing film and preservative film, can reduce the limitation of sealing, can seal glass culture dishes and plastic culture dishes of 6cm, 9cm and 12cm, does not need to be cut, is not easy to break and age, saves time and labor and greatly reduces the cost.
Detailed Description
The invention discloses a culture dish sealing method, which utilizes a winding film to seal a culture dish of 6-12 cm and comprises the following steps: the method comprises the following steps of sealing a 9cm glass culture dish by using a winding film: (1) cutting the winding film reel in length direction with a modified LZ-manual cutting machine into annular ring with width of 3cm, and sterilizing with ultraviolet rays for 30min on an ultraclean workbench; (b) and (3) taking a 9cm glass culture dish, sealing the cover opening by using the sterilized winding film in the step (1), closely pressing the winding film at the joint of the bottom edge of the culture dish and the cover opening during sealing, rotating the culture dish for 1.5-2 circles, and slightly pulling the winding film to break the winding film to finish sealing.
In the invention, the hand stretch wrapping film (also called PE stretch film) has higher tensile strength and tear strength, and good self-adhesion, and the adhesion is divided into single-sided adhesion and double-sided adhesion, so that an object can be wrapped into a whole. Meanwhile, the film has excellent transparency, and does not influence the growth of microorganisms needing illumination culture. Meanwhile, the composite material has good retractive force, the prestretching multiplying power is 400 percent, and the common specifications are as follows: the width is 30cm,35cm,45cm,50cm, and the thickness is 15um-50 um. According to the requirements of the invention, the steel plate needs to be cut into a width of 3cm by self.
The wound film is 50cm multiplied by 500m in price of 58 yuan, cut into annular rings with the width of 3cm according to requirements, and wound into 17 rolls, the total length is about 500m multiplied by 17 to 8500m, taking a culture dish with the diameter of 9cm as an example, 2 circles (or 1.5 circles) are needed for wrapping one culture dish, so the length is 3.14 multiplied by 9 multiplied by 2 to 57cm, the wound film can wrap 14912 culture dishes according to the specification, and the cost of each sealed 100 culture dishes is 0.39 yuan.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (3)
1. A method for sealing a culture dish utilizes a winding film to seal the culture dish of 6 cm-12 cm, and is characterized by comprising the following steps:
(1) cutting the length direction of a winding shaft of the winding film by using a modified LZ-manual cutting machine, cutting into an annular ring with the width of 3cm, and placing on a super-clean working table for sterilization;
(2) and (3) taking a 6-12 cm glass culture dish, sealing the cover opening by using the sterilized winding film in the step (1), closely pressing the winding film at the joint of the bottom of the culture dish and the cover opening during sealing, rotating the culture dish for 1.5-2 weeks, and then slightly pulling the winding film to break the winding film to finish sealing.
2. A method of closing a culture dish according to claim 1, wherein the culture dish is a glass or plastic culture dish.
3. The method of claim 1, wherein the wrapping film is a PE stretch film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910991185.XA CN110862915A (en) | 2019-10-18 | 2019-10-18 | Culture dish sealing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910991185.XA CN110862915A (en) | 2019-10-18 | 2019-10-18 | Culture dish sealing method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110862915A true CN110862915A (en) | 2020-03-06 |
Family
ID=69652361
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910991185.XA Pending CN110862915A (en) | 2019-10-18 | 2019-10-18 | Culture dish sealing method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110862915A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5770318A (en) * | 1995-01-13 | 1998-06-23 | Norton Performance Plastics Corporation | Thermoplastic seal and wrapping film |
| CN102217547A (en) * | 2011-06-01 | 2011-10-19 | 甘肃农业大学 | Opening sealing method of glass culture vessel for callus culture |
| CN205528759U (en) * | 2016-04-07 | 2016-08-31 | 中国水稻研究所 | Culture dish closing device |
-
2019
- 2019-10-18 CN CN201910991185.XA patent/CN110862915A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5770318A (en) * | 1995-01-13 | 1998-06-23 | Norton Performance Plastics Corporation | Thermoplastic seal and wrapping film |
| CN102217547A (en) * | 2011-06-01 | 2011-10-19 | 甘肃农业大学 | Opening sealing method of glass culture vessel for callus culture |
| CN205528759U (en) * | 2016-04-07 | 2016-08-31 | 中国水稻研究所 | Culture dish closing device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110862915A (en) | Culture dish sealing method | |
| CN204815983U (en) | A sterile air preparation system device for microbial fermentation | |
| CN204684261U (en) | A kind of medical apparatus and instruments automatic disinfection device | |
| CN104106464B (en) | The method of explant sterilization in a kind of Plant Tissue Breeding | |
| CN202954046U (en) | Venting culture dish with screw-cap | |
| CN203487140U (en) | Microbial cellulose production device | |
| CN214060478U (en) | A aseptic closing device that breathes freely of portable for laboratory tissue culture container | |
| CN205310358U (en) | Novel label tailor equipment | |
| CN211420121U (en) | Detection device for microorganisms in cosmetics | |
| CN212025330U (en) | Culture dish for microbial detection | |
| CN203538051U (en) | Plant culture and root system exudate collecting device | |
| CN211972310U (en) | High-efficient microbial fermentation culture composite set | |
| CN109488818B (en) | Water delivery pipe with antibacterial inner layer | |
| CN112094733A (en) | A aseptic closing device that breathes freely of portable for laboratory tissue culture container | |
| CN204930334U (en) | A kind of aquatic feeds Rapid Fermentation maker | |
| CN106754237A (en) | A kind of microbe rapid test piece | |
| CN214830304U (en) | Microbial limit inspection device | |
| CN205773936U (en) | A kind of device utilizing microbial metabolism purifying water body | |
| CN206077986U (en) | Take off diaphragm type plastic film residue collector and unload roller device | |
| JPH06239372A (en) | Air-permeable sterile container | |
| CN203017190U (en) | Medical dry film or medical wet film with fluid leaking seam | |
| CN207699591U (en) | A kind of biological cell culture apparatus that multigroup biological cell can be achieved and cultivates simultaneously | |
| CN216674196U (en) | Plant tissue culture box with multifunctional sealing film | |
| CN209507534U (en) | A kind of taper bottle capper with sterilizing function | |
| CN103385174B (en) | Candidum tissue culturing inoculation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200306 |
|
| RJ01 | Rejection of invention patent application after publication |