Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments. It is to be understood that the described embodiments are merely a few embodiments of the invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without inventive step, are within the scope of protection of the present invention.
In the description of the present invention, it is to be understood that the terms "central", "longitudinal", "lateral", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations and positional relationships based on those shown in the drawings, merely for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and therefore, are not to be considered limiting on the scope of the present invention.
Fig. 1-15 are schematic views of a reagent storage module according to some embodiments of the present invention. The reagent storage module is used for hermetically storing a reagent, in particular a liquid reagent.
Some embodiments provide a reagent storage module comprising a module body 1, wherein the module body 1 may be square or irregular in shape, etc., and may be arranged as desired.
Some embodiments provide a reagent storage module comprising a reagent cartridge 2 (shown in fig. 1), the reagent cartridge 2 being disposed within a module body 1 for hermetically storing a reagent. Adaptation to different detection systems can be achieved by varying the number of reagent cartridges 2 in the reagent storage module without the need to redesign the reagent storage module. Each reagent storehouse 2 in the reagent storage module is totally separated, can avoid mutual pollution and infiltration between the reagent in the reagent of long time is preserved, simple structure, the adjustment of being convenient for.
Some embodiments provide a reagent storage module comprising a first channel 3 (as shown in fig. 6), the first channel 3 being provided in the module body 1, a first end of the first channel 3 being in communication with the reagent cartridge 2. The second end of the first channel 3 is sealed with the outside of the module body 1 by the seal assembly 5, and the second end of the first channel 3 is communicated with the outside of the module body 1 in a state that the seal assembly 5 is broken and the seal assembly 5 is failed. The first channel 3 may be used to lead out the reagent in the reagent cartridge 2.
Some embodiments provide a reagent storage module comprising a second channel 4, the second channel 4 being disposed within the module body 1, a first end of the second channel 4 being in communication with the reagent cartridge 2. The second end of the second channel 4 is sealed with the outside of the module body 1 by the seal assembly 5, and the second end of the second channel 4 is in gas communication with the outside of the module body 1 in a state where the seal assembly 5 is broken and the seal assembly 5 fails. The second channel 4 can be used for injecting gas into the reagent bin 2 to ensure the air pressure balance in the reagent bin 2 and facilitate the outflow of liquid reagents in the reagent bin 2.
Some embodiments provide a reagent storage module comprising a sealing assembly 5 (as shown in fig. 4 and 6), the sealing assembly 5 being provided at the module body 1, the sealing assembly 5 being configured to close the second end of the first channel 3 to disconnect the second end of the first channel 3 from communication with the outside of the module body 1. The sealing assembly 5 also serves to close the second end of the second channel 4 to disconnect the second end of the second channel 4 from gas communication outside the module body 1.
In the event of failure of the sealing assembly 5: the second end of the first channel 3 communicates with the outside of the module body 1, and the first channel 3 is used for introducing the reagent in the reagent cartridge 2 to the outside of the module body 1.
In the event of failure of the sealing assembly 5: the second end of second passageway 4 and the outside gaseous intercommunication of module main part 1, second passageway 4 is used for leading the outside gas of module main part 1 to reagent storehouse 2 in to guarantee the atmospheric pressure balance in the reagent storehouse 2, do benefit to flowing out through first passageway 3 of the liquid reagent in the reagent storehouse 2.
Some embodiments provide a reagent storage module comprising a third channel 6 (as shown in fig. 6), the third channel 6 being provided in the module body 1, the first end of the first channel 3 being in communication with the reagent cartridge 2 via the third channel 6. I.e. the first end of the first channel 3 communicates with the second end of the third channel 6 and the first end of the third channel 6 communicates with the reagent cartridge 2.
In some embodiments, the flow area of the third channel 6 is smaller than or equal to the flow area of the first channel 3, and the flow area of the first channel 3 is matched with the needle head, so that the reagent in the reagent bin 2 can be completely led out, and the reagent residue is avoided.
In some embodiments, the first end of the third channel 6 is communicated with the reagent chamber 2 via the bottom of the reagent chamber 2, so that the reagent in the reagent chamber 2 can be completely led out, and the reagent residue can be avoided. The second end of the third channel 6 communicates with the first end of the first channel 3.
In some embodiments, the sealing assembly 5 comprises sealing gaskets 51 (shown in fig. 6), the sealing gaskets 51 being provided at the second end of the first channel 3 and the second end of the second channel 4 for sealing the second end of the first channel 3 from the exterior of the reagent cartridge 2 and for sealing the second end of the second channel 4 from the exterior of the reagent cartridge 2, respectively.
In some embodiments, the sealing pad 51 may be made of organic polymer materials such as rubber, ethylene propylene diene monomer, etc., and has a certain brittleness, and can be punctured by applying pressure with a sharp instrument; simultaneously, still possess certain elasticity, thereby the elastic deformation of accessible material self prevents that reagent from revealing with sharp ware inseparable parcel.
In some embodiments, the sealing assembly 5 includes a pressing piece 52 (as shown in fig. 6), the pressing piece 52 is used for pressing and fixing the sealing gasket 51 to the module body 1, and through holes are formed in the pressing piece 52 at positions corresponding to the second ends of the first channel 3 and the second channel 4. A sharp instrument can be inserted through the through-hole in the pressure plate 52 and into the seal 51.
In some embodiments, at least two independent reagent chambers 2 for hermetically storing reagents are arranged in the module main body 1. Each independent reagent cartridge 2 is provided with at least two channels, one channel is used for guiding reagent flow (a first channel 3), one channel is used for connecting external atmospheric equilibrium air pressure (a second channel 4), and the open ends of the channels (the second end of the first channel 3 and the second end of the second channel 4) are arranged on the side surface of the module main body 1.
In some embodiments, a groove structure or a boss structure may be provided at the position of the opening end of the channel, and the sealing assembly 5 cooperates with the groove structure or the boss structure to assist the sealing assembly 5 in sealing the channel. Which can be implemented using the various embodiments described below.
In some embodiments, the module body 1 comprises a first groove 11, the end of the second end of the first channel 3 being located within the first groove 11.
In some embodiments, the module body 1 comprises a second groove 12, the end of the second channel 4 being located within the second groove 12.
In some embodiments, the gasket 51 includes at least a region disposed within the first groove 11.
In some embodiments, the gasket 51 includes at least a region disposed within the second groove 12.
In some embodiments, the pressing piece 52 includes at least a region for compressively fixing the region of the sealing gasket 51 located in the first groove 11.
In some embodiments, the pressing piece 52 includes at least a region for compressively fixing the region of the sealing gasket 51 located in the second groove 12.
It will be appreciated that the gasket 51 may be a one-piece structure sized to match the size of the module body 1, covering the open ends of all of the channels on the module body 1, i.e., the second ends of the channels. Note: the first end of each channel communicates with the reagent tank 2, and the second end of each channel is an open end, and can communicate with the outside of the module main body 1 when the gasket 51 is broken.
Alternatively, the gasket 51 may be a split structure (as shown in fig. 4 and 6), and may include the following embodiments, for example.
In some embodiments, the gasket 51 includes a first gasket processed in a sheet shape. The first sealing gasket can be matched with the first groove 11 in external dimension and is arranged in the first groove 11.
In some embodiments, the gasket 51 includes a second gasket processed in a sheet shape. The second gasket may have a physical dimension matching the second groove and is disposed in the second groove 12.
Similarly, the pressing piece 52 may be a one-piece structure having a size matched to the size of the module body 1 for pressing and fixing the gasket 51 to the open ends of all the channels (i.e., the second ends of the channels) on the module body 1 to close the open ends of the channels from the outside of the module body 1.
Alternatively, the pressing plate 52 may be a split structure (as shown in fig. 4 and 6), and may include the following embodiments, for example.
In some embodiments, the pressing piece 52 includes a first pressing piece having a first portion having a size corresponding to the size of the first groove 11 and disposed in the first groove 11, and a second portion (as shown in fig. 5 and 6) disposed outside the first groove 11, and the first pressing piece is used for pressing the sealing gasket 51.
In some embodiments, the pressing piece 52 comprises a second pressing piece, which comprises a third portion (corresponding to the first portion of the first pressing piece) and a fourth portion (corresponding to the second portion of the first pressing piece), wherein the third portion has a size corresponding to the size of the second groove 12 and is disposed in the second groove 12, and the fourth portion is disposed outside the second groove 12, and the second pressing piece is used for pressing the gasket 51.
Optionally, the first pressing plate and the second pressing plate are provided with a downward-protruding structure at the central portion, and the length of the downward-protruding structure is matched with the depth of the first groove 11 and the second groove 12, so that the downward-protruding structure can be correspondingly sunk into the first groove 11 and the second groove 12.
Alternatively, the pressing piece 52 may be fixedly coupled to the module body 1 using a screw.
In some embodiments, the end of the second end of the first channel 3 is located in the first groove 11 of the module body 1, and the end of the second channel 4 is located in the second groove 12 of the module body 1; alternatively, the module body 1 may be provided with a boss structure instead of the groove structure (the first groove 11 and the second groove 12), and the second end of the first channel 3 and the second end of the second channel 4 may be provided on the boss structure provided on the module body 1. For example:
in some embodiments, the module body 1 includes a first boss 13, and the end of the second end of the first channel 3 is located at the first boss 13.
In some embodiments, the module body 1 includes a second boss 14, and the end of the second channel 4 is located at the second boss 14.
To accommodate the above-described boss structures (the first boss 13 and the second boss 14), the gasket 51 may be a cap-type structure capable of surrounding the above-described boss structures.
Alternatively, the gasket 51 may be an integral structure (as shown in fig. 15) with a cap-shaped structure formed at a position corresponding to the boss structure, or the gasket 51 may be a separate structure (as shown in fig. 12), and each separate gasket 51 is a cap-shaped structure and surrounds a boss. The cap-shaped structure of the gasket 51 may be sized to fit tightly over the boss structure.
The form of the gasket 51 may include the following embodiments.
In some embodiments, gasket 51 includes at least an area for sealingly enclosing first boss 13 therein.
In some embodiments, gasket 51 includes at least an area for sealingly enclosing second boss 14 therein.
In some embodiments, gasket 51 comprises a first gasket that sealingly surrounds first boss 13.
In some embodiments, gasket 51 comprises a second gasket that sealingly surrounds second boss 14.
Similarly, to accommodate the boss configuration described above, the wafer 52 may be a cap-type configuration that surrounds the boss.
Alternatively, the pressing plate 52 may be a one-piece structure (as shown in fig. 12 and 15) with a cap-shaped structure formed at a position corresponding to the boss structure, or the pressing plate 52 may be a split structure, and each split pressing plate 52 is a cap-shaped structure and correspondingly surrounds a boss. The cap-shaped structure of the pressing piece 52 matches the cap-shaped structure of the sealing gasket 51.
The form of the preform 52 may include the following examples.
In some embodiments, the module body 1 includes a press tab 52. The pressing piece 52 includes at least a region for press-fixing the gasket 51 to the first boss 13.
In some embodiments, the pressing piece 52 includes at least a region for press-fixing the gasket 51 to the second boss 14.
In some embodiments, the pressing piece 52 includes a first pressing piece including a first receiving portion recessed upward from the bottom, similar to a cap-type structure, for receiving the first boss 13 and the sealing gasket 51.
In some embodiments, the pressing piece 52 includes a second pressing piece including a second receiving portion recessed upward from the bottom, similar to a cap-type structure, for receiving the second boss 14 and the sealing gasket 51.
During assembly, the sealing gasket 51 may be first combined with the pressing sheet 52 from the bottom of the pressing sheet 52, so that the cap structure on the sealing gasket 51 is pressed into the cap structure on the pressing sheet 52, and then the whole of the sealing gasket 51 and the pressing sheet 52 is fixed to the module body 1 filled with the reagent, so as to seal the second end of each channel on the module body 1 from the outside of the module body 1, thereby sealing the reagent chamber 2.
The boss structure is arranged on the module main body 1, so that the step of placing the sealing gasket 51 into the groove on the module main body 1 is omitted, the sealing gasket 51 is sleeved on the boss structure, and then the pressing sheet 52 is sleeved on the boss structure, or the sealing gasket 51 and the pressing sheet 52 can be combined firstly and then combined and fixed with the module main body 1, the processing technology and operation can be simplified, and convenience is brought to the flow line design.
In the embodiment in which the pressing piece 52 is provided as an integral structure, it is advantageous to reduce the number of screws for connecting the pressing piece 52 to the module body 1.
Alternatively, the pressing plate 52 may be connected to the module body 1 by using a fastening mechanism such as a snap, an expansion screw, a snap spring, etc. to fasten the pressing plate 52 to the module body 1.
In some embodiments, the size of the cap-shaped structure of the pressing sheet 52 may be slightly smaller than that of the boss structure, and the pressing sheet 5 may be surrounded and pressed to the boss structure to be fixed to the boss structure, so that the locking process of screws is omitted, the processing technology and the assembly operation of the chip are greatly simplified, an optimized scheme is provided for the design of the production line, and the clinical feasibility is greatly improved.
In some embodiments, the gasket 51 is shaped to mate with a groove or land structure at the open end of the channel to effect a seal against the open end of the channel.
In some embodiments, the shape of the pressing piece 52 is matched with the groove structure or the boss structure on the module body 1 to apply additional pressure to the periphery of the gasket 51 fixed thereto to enhance the sealing performance thereof. Meanwhile, the center of the pressing piece 52 is a through hole, through which the center part of the sealing pad 51 can be exposed, so as to facilitate the piercing of the sealing pad 51 from the through hole for leading out the reagent.
In some embodiments, the reagent chamber 2 is an elongated shape, and has a first end connected to the first channel 3 and a second end connected to the second channel 4.
In some embodiments, the outer edge of the first end of the reagent chamber 2 is flush and is communicated with the third channel 6, which is beneficial to completely leading out the reagent in the reagent chamber 2 and preventing the reagent from remaining. The outer edge of the second end of the reagent chamber 2 is arc-shaped (as shown in fig. 7, 10 and 13), which is beneficial to matching and processing with the second channel 4.
In some embodiments, the reagent storage module comprises more than two reagent compartments 2, each reagent compartment 2 being provided with a first channel 3, a second channel 4 and a sealing assembly 5. Namely, at least two independent reagent chambers 2 are arranged in the module main body 1. At least two channels are configured for each independent reagent chamber 2, and are used as a flow channel and an air flow channel of the reagent chamber 2.
In some embodiments, only one reagent storage module is needed to realize one-time filling and sealing of different liquid reagents, the structure is simple, the size of the module main body 1 and the size of the reagent bin 2 can be adjusted according to the types and the quantity of the reagents and the volume of the reagents, so that various experimental requirements are met, and the design, processing and manufacturing efficiency of the microfluidic chip is greatly improved.
In some embodiments, the module body 1 comprises a bottom sheet and a substrate, wherein the substrate is provided with a groove for forming the reagent chamber 2, and the substrate is provided with a channel communicated with the reagent chamber 2. The bottom plate is arranged at the bottom of the substrate and is used for sealing the reagent bin 2 and the channel on the substrate.
Alternatively, the substrate of the module body 1 is attached to the bottom plate by bonding means such as adhesive, heat pressing, ultrasonic bonding, etc.
Alternatively, the material of the bottom sheet of the module main body 1 may be a hard or soft organic polymer material.
The reagent storage module provided by some embodiments is reliable and durable, and has a good sealing effect; the bonding mode and the sealing mode are simple and reliable, the selected materials are corrosion-resistant, high-temperature-resistant and wear-resistant, and the leakage and deformation of the test agent can be avoided in long-term storage.
Some embodiments provide a combination of a reagent storage module and a communicator, comprising the reagent storage module described above and a communicator 7.
The reagent storage module is used for realizing sealed storage and long-term preservation of the reagent. The communicating vessel 7 is used for breaking the sealing component 5, opening the reagent bin 2 in the reagent storage module and leading out the reagent, and sealing the reagent in the process of leading out the reagent.
In some embodiments, the communicator 7 includes a needle plate 71 and a needle 72 (shown in fig. 9).
Wherein the needle plate 71 is provided with through holes and passages communicating with the through holes.
The needle 72 is arranged on one side of the needle plate 71 and is communicated with the through hole on the needle plate 71, and the needle 72 is used for puncturing the sealing assembly 5 so as to be communicated with the reagent bin 2.
In some embodiments, the reagent storage module seals the outlet end of the first channel 3 communicated with the reagent chamber 2 through the sealing gasket 51, and the pressing sheet 5 is used to reinforce and fix the sealing effect of the sealing gasket 51. When it is desired to open the seal, directional release of the liquid agent can be achieved by piercing the seal 51 with the needle 72.
In some embodiments, the circumferential dimension within the first channel 3 coincides with the outer circumferential dimension of the needle 72, facilitating complete exit of the agent.
In some embodiments, the communicator 7 comprises a needle plate 71 and a needle 72, the needle 72 at least being used to pierce the sealing assembly 5 of the reagent storage module to draw the reagent in the reagent cartridge 2 in the module body 1 out through a channel on the needle plate 71 to a predetermined position.
Alternatively, the needle 72 may be a hollow sharp needle. The hollow sharp instrument needle head is a hollow inclined needle head, one end of the hollow sharp instrument needle head is of an inclined plane structure with a thinner diameter and puncture capability, and the hollow sharp instrument needle head is the top end; the other end is a fixed structure with a larger diameter and matched with the through hole on the needle plate 71 and is the bottom end.
Alternatively, the needle 72 may employ a plastic needle instead of a metal needle. Further, the needle 72 can be directly formed integrally with the needle plate 71 by injection molding or high-precision machining.
In some embodiments, the needle head 72 is a plastic needle and is integrally formed with the needle plate 71, so that the assembly process of the needle head 72 and the needle plate 71 is omitted, the processing technology and the assembly operation of the communicating vessel 7 are greatly simplified, and meanwhile, the plastic needle is used for replacing a metal needle and mold opening and injection molding are realized, so that the production cost is greatly reduced; an optimization scheme is provided for the design of the production line, so that the scheme is greatly improved according to the clinical feasibility.
In some embodiments, the needle plate 71 of the communicating vessel 7 can also be formed by attaching the base sheet and the base sheet. Alternatively, the needle plate 71 of the communicating vessel 7 may be made of the same material as the module body 1 of the reagent storage module.
In some embodiments, the communicating vessel 7 and the reagent storage module are sealed during the reagent extraction process by using the deformation of materials.
In some embodiments, in order to match a configuration in which the reagent storage module comprises more than two reagent chambers 2, each needle 72 of the communicating vessel 7 may be fixed on the needle plate 71, and the needle plate 71 is provided with a plurality of channels for communicating the needles 72 to be connected to form a fluid flow network.
In an embodiment of the module body 1 with a groove structure, the substrate and the bottom sheet of the module body 1 are attached, different reagents are respectively injected into the reagent chamber 2 from the liquid flow channel, the sealing pad 51 is placed in the groove structure to cover the outlet end of the channel, then the downward protruding structure of the pressing sheet 52 is aligned with the groove structure of the module body 1 and pressed in, and finally the screw is locked to complete the sealing of the reagents.
When the sealing is required to be opened to release the reagent, the needle 72 on the communicating vessel 7 is aligned with the through hole on the pressing sheet 52 of the assembled reagent storage module, and is pressed down forcibly, the sharp surface of the needle is used to pierce the sealing pad 51 into the channel, and the sealing pad 51 and the channel form a seal to prevent the reagent from overflowing. At this time, the reagent can flow out through the pierced hollow needle 72, and the external air pressure can be balanced by the needle 72 of the air pressure balancing passage connected to the reagent chamber 2, so as to ensure the smooth outflow of the liquid reagent.
Some embodiments provide a reagent storage module and a communicating vessel that are easy to combine, and the overall combination and application steps are simple and easy to implement, and can be operated only by a manual or external instrument pressure lever.
Some embodiments provide a microfluidic chip including the reagent storage module described above, or a combination of the reagent storage module described above and a connector.
The reagent storage module provided by some embodiments has a simple structure, can be rapidly molded through mold opening, and meanwhile, the reagent storage module has extremely strong universality, the size and the number of the reagent bins 2 can be adjusted according to needs, the production and processing cost of the microfluidic chip can be greatly reduced, and the clinical application value of the microfluidic chip is improved; the problems of complex design, difficult processing, low yield, high cost and poor applicability of the traditional microfluidic chip reagent storage, sealing and opening mechanism are solved.
Three embodiments of the present invention are further described below with reference to FIGS. 1-15.
Fig. 1 shows the main configuration of the module body 1 with the single reagent cartridge 2 described above. The inside of the module main body 1 is provided with a reagent cabin 2 for storing liquid reagent and is connected with the surface of the module main body 1 through two channels. The open ends of the two channels are located in the groove structure of the module body 1 to seal with the gasket 51 and the pressing piece 52.
Fig. 2 to 9 show a first embodiment. In the first embodiment, the module body 1 is provided with a first groove 11 and a second groove 12, which are respectively located at a liquid flow opening end (second end) of the first channel 3 and an air flow opening end (second end) of the second channel 4 which are communicated with the reagent chamber 2. After reagent is injected through the first channel 3, the first sealing gasket and the second sealing gasket are respectively and correspondingly arranged in the first groove 11 and the second groove 12 to cover the second end of the first channel 3 and the second end of the second channel 4, the first pressing sheet and the second pressing sheet are respectively and correspondingly fastened to the module main body 1, and the first sealing gasket and the second sealing gasket are respectively and correspondingly pressed through the first pressing sheet and the second pressing sheet to realize the sealing of the reagent bin 2. When the reagent chamber 2 is opened, the reagent chamber 2 can be opened to release the liquid reagent by puncturing the first sealing gasket and the second sealing gasket with the hollow needle 72.
Fig. 10 to 12 show a second embodiment. In the second embodiment, the module body 1 is provided with a first boss 13 and a second boss 14, which are respectively located at the liquid flow opening end (second end) of the first channel 3 and the gas flow opening port (second end) of the second channel 4 of the reagent cartridge 2. Injecting a reagent through the first channel 3, correspondingly buckling the first sealing gasket and the second sealing gasket of the cap type on the first boss 13 and the second boss 14 respectively after the reagent is injected, and then enhancing the sealing effect of the first sealing gasket and the second sealing gasket by the pressing sheet 52 with the corresponding cap type structure. When the reagent chamber 2 is opened, the hollow needle 72 is also used to puncture the first sealing gasket and the second sealing gasket, so that the reagent chamber 2 can be opened to release the liquid reagent.
Fig. 13 to 15 show a third embodiment in which a single-piece capped gasket 51 is used instead of the first and second gaskets of the second embodiment, and the sealing of the reagent cartridge 2 is completed by assembling the gasket 51 and the pressing piece 52 and then assembling the gasket and the module body 1.
Further description of the structural dimensions of the three embodiments described above will be made below.
In the module body 1 of the above three embodiments, the substrate is a rectangular structure of 31mm × 10mm × 8mm, a channel with a depth of 0.5mm and a reagent chamber with a volume of about 300uL are dug on one side of the substrate, and through holes are respectively drilled at the ends of the pipeline and the reagent chamber. The surface of the substrate with the reagent chamber is bonded with the bottom plate by bonding modes such as gluing, thermal bonding, ultrasonic bonding and the like to form a sealed reagent chamber 2. The difference between the three embodiments is the structural difference of the open ends of the channels.
In the first embodiment, the module body 1 is provided with a groove structure with a diameter of 8mm and a depth of 2mm at both the liquid flow opening end and the gas flow opening end. The diameter of the two sheet-like sealing gaskets 51 which cooperate with them is 8mm and the thickness is 0.5 mm. The sealing gasket 51 in this embodiment is produced by using nitrile butadiene rubber as a raw material. In this embodiment, the pressing sheet 52 is a square plate with a central convex structure. The whole is made for the PC material, and the bottom size of protruding structure is 10mm x 2mm down, and the through-hole of 4mm aperture is opened at the center, and protruding structure is all around the through-hole, and height 1mm, wall thickness 8 mm. The implementation of this embodiment is, pours into reagent into the reagent storehouse through the air current open end, then puts into sealed pad 51 in groove structure, later imbeds preforming 52 again, fixes preforming 52 through methods such as gluing, screw fastening, buckle, can realize reagent storehouse and deposit the sealed of module. When the connector is opened, the through hole of the pressing piece 52 is aligned with the connector 7 to penetrate.
In the second embodiment, the groove structure on the module body 1 is changed into a boss structure, the wall thickness is 1mm, and the height is 1 mm. Accordingly, both the packing 51 and the pressing piece 52 are structurally changed. The sealing gasket 51 is replaced by a cap-shaped sealing gasket matched with two boss structures, the material is still butadiene-acrylonitrile rubber, the diameters of the two sealing gaskets 51 are respectively 6mm and 10mm, and the thickness of each sealing gasket is 1.5 mm. The depth of the two sealing gaskets in the caps is 0.8mm, and the inner diameters of the two sealing gaskets are 4mm and 8mm respectively. The pressing sheet 52 is a rectangular PC board with the size of 33mm multiplied by 12mm multiplied by 2mm, two bulges which are matched with the boss structure and are upwards from the bottom are arranged on the pressing sheet, the cross section diameters of the bulges are respectively 6mm and 10mm, the height of the bulges is 1mm, and a through hole is arranged in the center of the bulges. The implementation manner of this embodiment is to inject the reagent into the reagent chamber 2 through the opening end of the air flow, then put the cap-shaped sealing gasket 51 into the pressing sheet 52 for compressing, then buckle the pressing sheet 52 onto the module body 1 for fastening, and fix the pressing sheet 52 and the module body 1 by methods such as gluing, screw fastening, and buckling, so as to realize the sealing of the reagent chamber storage module. When the connector is opened, the through hole of the pressing sheet 52 is aligned with the connector 7 to penetrate.
In the third embodiment, the module body 1 has the same outer dimensions as those of the second embodiment. The gasket 51 was replaced with a one-piece sheet of butadiene rubber film having dimensions of 31mm × 10mm × 1mm for ease of assembly. The part of the sealing gasket 51 corresponding to the boss structure of the module body 1 is distributed with two cap-shaped protrusions with the diameters of 8mm and 4mm respectively, and the internal height of the cap-shaped protrusions is 0.8 mm. The diameters of the cross sections of the cap-shaped protrusions are respectively 10mm and 6mm, and the height of the cap-shaped protrusions is 1 mm. Correspondingly, in order to facilitate the assembly and implementation of the chip, the pressing sheet 52 may be modified to be a whole sheet having dimensions of 33mm × 12mm × 2 mm. At the position corresponding to the cap-shaped protrusion of the sealing gasket 51, two protrusions with the cross-sectional diameters of 10mm and 6mm and the depth of 0.8mm are distributed on the pressing sheet 52, and a through hole with the aperture of 2mm is arranged at the center of the protrusion of the pressing sheet 52. The reagent cabin module is realized by injecting reagent into the reagent cabin 2 through the opening end of the airflow, then paving the sealing pad 51 on the module main body 1, positioning and fixing through the cap-shaped structure and the boss structure on the module main body 1, then buckling the pressing sheet 52 on the sealing pad 51 for buckling, and fixing the pressing sheet 52 and the module main body 1 by using methods such as gluing, screw fastening, buckling and the like, so that the sealing of the reagent cabin module can be realized. When the connector is opened, the through hole of the pressing piece 52 is aligned with the connector 7 to penetrate.
In the above three embodiments, the communicating vessel 7 comprises the needle 72 and the needle plate 71, and the needle plate 71 is a square PC plate with a diameter of 10mm × 10mm × 2mm, and a through hole is arranged in the center. A needle 72 with a length of 8mm is arranged on one surface of the needle plate 71, the needle 72 can be selected to be a sharp needle, the bevel angle of the puncturing end of the needle 72 is 45 degrees, and the needle wall thickness is 0.5 mm.
The chip provided by some embodiments is common in material selection, low in price, and millimeter in channel design size, can realize large-scale mold opening injection molding, and is easy to realize mass production and manufacturing.
In the description of the present invention, it should be understood that the terms "first", "second", "third", etc. are used to define the components, and are used only for the convenience of distinguishing the components, and if not otherwise stated, the terms have no special meaning, and thus, should not be construed as limiting the scope of the present invention.
Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present invention and not to limit the same; although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art will understand that: modifications to the specific embodiments of the invention or equivalent substitutions for parts of the technical features may be made; without departing from the spirit of the present invention, it is intended to cover all aspects of the invention as defined by the appended claims.