CN110856814B

CN110856814B - Reaction cavity module and micro-fluidic chip

Info

Publication number: CN110856814B
Application number: CN201810957452.7A
Authority: CN
Inventors: 葛胜祥; 张师音; 苏晓崧; 张东旭; 闵小平; 张建中; 张雅; 郭清顺; 张军; 夏宁邵
Original assignee: Xiamen Innodx Biotechnology Co ltd; Xiamen University
Current assignee: Xiamen Innodx Biotechnology Co ltd; Xiamen University
Priority date: 2018-08-22
Filing date: 2018-08-22
Publication date: 2020-11-03
Anticipated expiration: 2038-08-22
Also published as: CN110856814A

Abstract

The invention relates to a reaction cavity module and a micro-fluidic chip. Wherein, the reaction chamber module includes: a module body; the reaction cavity is arranged in the module main body, and the bottom of the reaction cavity protrudes downwards; the liquid flow channel is arranged in the module main body, the first end of the liquid flow channel is communicated with the reaction cavity through the lowest end of the bottom of the reaction cavity, and the second end of the liquid flow channel is communicated with the outside of the module main body; and the airflow channel is arranged in the module main body, the first end of the airflow channel is in gas communication with the reaction cavity through the top of the reaction cavity, and the second end of the airflow channel is in gas communication with the outside of the module main body. The reaction chamber of the present invention may be formed as a large volume space for holding and providing reaction space support for the reaction reagents.

Description

Reaction cavity module and micro-fluidic chip

Technical Field

The invention relates to the field of microfluidic detection, in particular to a reaction cavity module and a microfluidic chip.

Background

Due to its high integration and strong automation, the microfluidic chip technology is increasingly applied to point-of-care testing (POCT) in clinical testing projects. In order to transplant the existing reagent system to the microfluidic platform, a reaction cavity with a corresponding large volume is required for supporting.

The problems with large volume reaction chambers are: 1) the excessive reaction cavity volume is not beneficial to the uniform input of the reagent, and the bubble blockage is easy to occur, so that the uneven distribution of the reaction system in the reaction cavity is caused; 2) the general large-volume reaction chamber is difficult to completely discharge waste liquid, so that the waste liquid in the reaction chamber is remained, the dosage of washing liquid is increased, and wrong detection results are easily caused.

At present, manufacturers of microfluidic detection systems at home and abroad mainly adopt a flexible reaction chamber or a micro reaction chamber to solve the problem. But the sample cracking efficiency of the flexible reaction cavity is lower, and the instrument control is more complex; the micro reaction cavity needs to be matched with a sample enrichment membrane with a patented technology for use, and the cost is extremely high.

Disclosure of Invention

One of the objectives of the present invention is to provide a reaction chamber module and a microfluidic chip which are beneficial to the waste liquid drainage.

To achieve the above object, the present invention provides a reaction chamber module, comprising: a module body; the reaction cavity is arranged in the module main body, and the bottom of the reaction cavity protrudes downwards; the liquid flow channel is arranged in the module main body, the first end of the liquid flow channel is communicated with the reaction cavity through the lowest end of the bottom of the reaction cavity, and the second end of the liquid flow channel is communicated with the outside of the module main body; and the airflow channel is arranged in the module main body, the first end of the airflow channel is in gas communication with the reaction cavity through the top of the reaction cavity, and the second end of the airflow channel is in gas communication with the outside of the module main body.

Optionally, the reaction cavity module comprises an energy guide structure, which is arranged at the outer side of the module body where the reaction cavity is located, and is used for contacting with a device for providing vibration energy into the reaction cavity so as to transfer the energy into the reaction cavity.

Optionally, the reaction chamber includes a cylindrical reaction chamber, a radial direction of the cylindrical reaction chamber is a direction between a top and a bottom of the reaction chamber, and an axial direction of the cylindrical reaction chamber is a direction between opposite side portions of the reaction chamber.

Optionally, the liquid flow channel comprises a channel section tangential to the bottom of the cylindrical reaction chamber, and/or the gas flow channel comprises a channel section tangential to the top of the cylindrical reaction chamber.

Optionally, the reaction cavity module comprises a buffer part, one end of the buffer part is tangent to the top of the cylindrical reaction cavity, and the other end of the buffer part is communicated with the cylindrical reaction cavity.

Optionally, the reaction chamber module comprises an energy guide structure, which is disposed outside the module body and on the central axis of the cylindrical reaction chamber, and is used for contacting with a device for providing vibration energy into the cylindrical reaction chamber to transfer energy into the cylindrical reaction chamber.

Optionally, the energy guiding structure is cylindrical, conical or hemispherical.

Optionally, the energy guiding structure includes a wall surface protruding outward and disposed on one side of the module body at a position corresponding to the reaction chamber.

Optionally, the reaction chamber module comprises a filter chamber disposed in the gas flow channel, and filled with a material for filtering gas.

Optionally, more than one reaction chamber is arranged in the module main body, and each reaction chamber is provided with the liquid flow channel and the gas flow channel.

In order to achieve the above object, the present invention provides a microfluidic chip, which includes the reaction chamber module.

Optionally, the microfluidic chip comprises a device for providing vibrational energy into a reaction chamber in said reaction chamber module.

Optionally, the device for providing vibrational energy comprises an ultrasonic transducer, an eccentric vibrator or an electromagnetic transducer.

Based on the technical scheme, the invention at least has the following beneficial effects:

in some embodiments, a reaction cavity is arranged in the module main body, the bottom of the reaction cavity protrudes downwards, waste liquid is discharged from the bottom of the reaction cavity, and the reaction cavity can be made into a large-capacity volume space for accommodating reaction reagents and providing reaction space support for the reaction reagents.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without limiting the invention. In the drawings:

fig. 1(a) is a schematic front view of a substrate of a reaction chamber module according to some embodiments of the invention.

Fig. 1(b) is a schematic rear view of a substrate of a reaction chamber module according to some embodiments of the invention.

FIG. 2 is a schematic diagram of an assembly of a reactor module according to some embodiments of the invention.

Fig. 3 is a schematic side view of a substrate according to some embodiments of the invention.

FIG. 4 is a schematic assembly diagram of a reaction chamber module according to another embodiment of the present invention.

Fig. 5(a) is a schematic diagram of a first energy guiding structure of a reaction chamber module according to some embodiments of the invention.

Fig. 5(b) is a schematic cross-sectional view of fig. 5 (a).

Fig. 6(a) is a schematic diagram of a second energy guiding structure of a reaction chamber module according to some embodiments of the invention.

Fig. 6(b) is a schematic cross-sectional view of fig. 6 (a).

Fig. 7(a) is a schematic diagram of a third energy guiding structure of a reaction chamber module according to some embodiments of the invention.

Fig. 7(b) is a schematic cross-sectional view of fig. 7 (a).

Fig. 8(a) is a schematic diagram of a fourth energy guiding structure of a reaction chamber module according to some embodiments of the invention.

Fig. 8(b) is a schematic cross-sectional view of fig. 8 (a).

FIG. 9 is a schematic diagram of a reaction chamber module including a buffer tank according to some embodiments of the invention.

FIG. 10 is a schematic diagram of a reactor module and apparatus for providing vibrational energy in accordance with certain embodiments of the present invention.

Fig. 11 is a partial cross-sectional schematic view of fig. 10.

The reference numbers in the drawings:

1-a module body; 11-a substrate; 12-a back plate;

2-a reaction chamber;

3-a flow channel; 31-a first via;

4-an airflow channel; 41-a second through hole;

5-an energy conducting structure;

6-a buffer part;

7-a filter chamber; 71-filtration of the filling;

8-devices for providing vibrational energy.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments. It is to be understood that the described embodiments are merely a few embodiments of the invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without any inventive step, are within the scope of the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships based on those shown in the drawings, and are used merely for convenience in describing the present invention and for simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be taken as limiting the scope of the present invention.

FIGS. 1-9 are schematic views of a reaction chamber module according to some embodiments.

In some embodiments, as shown in fig. 1(a), 1(b), the reaction chamber module comprises a module body 1.

In some embodiments, the reaction cavity module comprises a reaction cavity 2, the reaction cavity 2 is arranged in the module body 1, and the bottom of the reaction cavity 2 protrudes downwards, so that waste liquid is discharged from the bottom of the reaction cavity 2.

Because the bottom of the reaction chamber 2 protrudes downwards, the waste liquid is discharged from the bottom of the reaction chamber 2, and therefore, the reaction chamber 2 can be made into a large-capacity volume space for accommodating the reaction reagent and providing reaction space support for the reaction reagent.

In some embodiments, as shown in fig. 1(a), the reaction chamber module comprises a flow channel 3, the flow channel 3 being provided within the module body 1. The first end of the flow channel 3 communicates with the reaction chamber 2 via the lowermost end of the bottom of the reaction chamber 2. The second end of the flow channel 3 communicates with the outside of the module body 1. Further, the second end of the flow channel 3 is located above the reaction chamber 2. The liquid flow channel 3 is used for introducing a reaction reagent into the reaction chamber 2 and is used for leading out the reagent in the reaction chamber 2.

In some embodiments, the reaction chamber module includes gas flow channels 4, the gas flow channels 4 being provided within the module body 1. A first end of the gas flow channel 4 is in gas communication with the reaction chamber 2 via the top of the reaction chamber 2, and a second end of the gas flow channel 4 is in gas communication with the outside of the module body 1. Further, the second end of the gas flow channel 4 is located above the reaction chamber 2. The gas flow channel 4 is used to equalize the gas pressure in the reaction chamber 2.

The reaction cavity 2 in the embodiment is combined with the liquid flow channel 3 and the gas flow channel 4, so that the problem that waste liquid in a large-capacity reaction cavity is difficult to discharge can be solved, and large-capacity reagent mixing and reaction can be realized; the device has the functions of accommodating a reaction system, uniformly mixing the reaction system and discharging waste liquid, has a wide application range and is compatible with most biochemical reactions.

In some embodiments, more than one reaction chamber 2 may be disposed in the module body 1 of the reaction chamber module, and each reaction chamber 2 is provided with a liquid flow channel 3 and a gas flow channel 4. The volume of the reaction cavity 2 can be adjusted according to the needs, the structure is simple, the universality is strong, the manufacturing cost of the micro-fluidic chip is greatly reduced, the application range of the micro-fluidic chip is expanded, the industrial batch production of the micro-fluidic chip is favorably realized, and the method is suitable for the field of clinical detection.

In some embodiments, as shown in FIG. 2, the module body 1 includes a base plate 11 for forming a reaction chamber body and a back plate 12 for sealing. Alternatively, the substrate 11 may be bonded to the back plate 12 by gluing, ultrasonic bonding, thermal bonding, or the like.

As shown in fig. 2, a surface of the substrate 11, which is attached to the back plate 12, is recessed into the substrate 11 for forming the reaction chamber 2 in cooperation with the back plate 12, and the depth and size of the recess correspond to the corresponding detection reaction system. Alternatively, the cross-section of the groove may be circular.

The base plate 11 is further provided with a first strip-shaped groove for forming the liquid flow channel 3, which is dug toward the inside of the base plate 11, and a second end of the first strip-shaped groove is provided with a first through hole 31 (as shown in fig. 1 (b)), and the first through hole 31 is used for communicating with an external device. A second strip-shaped groove for forming the airflow channel 4 is further dug towards the inside of the substrate 11 on the substrate 11, a second end of the second strip-shaped groove is provided with a second through hole 41 (as shown in fig. 1 (b)), and the second through hole 41 is used for ventilating the reaction chamber 2 so as to balance the air pressure in the reaction chamber 2.

In some embodiments, as shown in fig. 4, the reaction chamber 2 comprises a cylindrical reaction chamber, the radial direction of the circular cross section of the cylindrical reaction chamber is the direction between the top and the bottom of the reaction chamber 2, and the axial direction of the cylindrical reaction chamber is the direction between the opposite side portions of the reaction chamber 2.

In some embodiments, the back plate 12 is a hard surface, and the substrate 11 bonded to the back plate 12 on the other side is an elastic surface. Alternatively, the substrate 11 is made of plastic material, a cylindrical groove is dug into the substrate 11 from one side to serve as the main body of the reaction chamber, and since the radial direction of the circular cross section of the cylindrical groove is the top and bottom directions of the reaction chamber 2, two pipelines are respectively led out from the bottom (right below) and the top (right above) of the reaction chamber 2 to serve as the liquid flow channel 3 and the gas flow channel 4. Then, as shown in fig. 2, the substrate 11 and the back plate 12 are bonded and assembled.

In some embodiments, the flow channel 3 comprises a channel section tangential to the bottom of the cylindrical reaction chamber.

In some embodiments, the gas flow channel 4 comprises a channel section tangential to the top of the cylindrical reaction chamber. Alternatively, the gas flow channel 4 is directly connected to the uppermost point of the top of the cylindrical reaction chamber.

In some embodiments, as shown in fig. 9, the reaction chamber module includes a buffer part 6, the buffer part 6 is a cavity body disposed at the top of the reaction chamber 2, one end of the buffer part 6 is tangent to the top of the cylindrical reaction chamber, and the other end is communicated with the cylindrical reaction chamber. Further, the depth of the buffer 6 is smaller than the depth of the reaction chamber 2.

Alternatively, in some embodiments, the reaction chamber 2 has a cylindrical lower half and a square upper half. The two sides of the square structure of the upper half of the reaction chamber 2 also correspond to the buffer parts 6, and can play a role of buffering.

In some embodiments, by providing the buffer part 6 at the top of the reaction chamber 2, it is advantageous to suppress the problem of leakage of the reagent from the gas flow channel 4 due to the capillary phenomenon and the edge effect, which may occur. Or, the cylindrical reaction chamber is set to be a structure with a cylindrical lower half part and a square upper half part, so that the reaction system loss caused by the edge effect and the capillary effect is inhibited.

In some embodiments, as shown in fig. 5 to 8, the reaction chamber module includes an energy guiding structure 5, the energy guiding structure 5 is disposed on the outer side of the module body 1 where the reaction chamber 2 is located, and protrudes toward the outer side of the module body 1 for contacting with a device 8 for providing vibration energy into the reaction chamber 2 to transfer the energy into the reaction chamber 2.

Under the condition of external force assistance of equipment 8 for providing vibration energy and the like, the efficient uniform mixing of various reagent components in the reaction cavity 2 is facilitated; the reaction efficiency is high. The energy conduction structure 5 combined with energy conduction can efficiently transfer the vibration energy provided by the external device 8 into the reaction cavity 2 to assist the reaction. The problem of too big reaction chamber volume be unfavorable for the even input of reagent, take place the bubble easily and block up thereby cause the inhomogeneous distribution of reaction system in the reaction chamber is solved.

Further, the energy guiding structure 5 is disposed on the surface of the back plate 12 to enhance the efficiency of external energy introduction.

In some embodiments, the energy conducting structure 5 is provided outside the module body 1 and on the central axis of the cylindrical reaction chamber for contacting the device 8 for providing vibrational energy into the cylindrical reaction chamber for transferring energy into the cylindrical reaction chamber. Energy guide structure 5 cooperates with equipment 8 that provides vibration energy, and energy guide structure 5 is located cylindrical reaction chamber's axis, does benefit to and realizes the high-efficient conduction of outside energy, and the reaction chamber is cylindrical and does benefit to energy conduction even.

In some embodiments, the energy directing structure 5 is cylindrical (as shown in fig. 5(a), 5 (b)). The radius of the cylindrical energy guiding structure 5 is not greater than 1/3 of the radius of the cylindrical reaction chamber 2. A small cylinder (energy guide structure 5) is arranged at the circle center of one side of the hard surface of the reaction cavity 2 and is used as a contact point with a device 8 for providing vibration energy, so that the efficiency of guiding the energy can be enhanced, and the reaction efficiency is improved.

In some embodiments, the energy guiding structure 5 may also be conical (as shown in fig. 6(a), 6 (b)).

In some embodiments, the energy guiding structure 5 may also be hemispherical (as shown in fig. 7(a), 7 (b)).

In some embodiments, the energy guiding structure 5 may also be an outwardly convex wall surface (as shown in fig. 8(a) and 8 (b)) provided at a position corresponding to the reaction chamber 2 of the module body 1 to increase energy conduction efficiency. The energy guiding structure 5 can be realized by processing the hard surface (back plate 12) into a convex wall surface. The radius of the convex wall surface can be less than or equal to the radius of the reaction cavity main body.

It can be understood that the energy guide structure 5 is a structure protruding outward and capable of reducing the contact area with the external device 8, and can guide the auxiliary energy such as ultrasound provided by the external device 8 into the reaction chamber 2 more efficiently to assist the reaction.

As shown in fig. 10 and 11, when the reaction chamber module works, the energy guide structure 5 is in close contact with the probe of the ultrasonic transducer (belonging to the device 8 for providing vibration energy), so that the energy of the ultrasonic transducer or other transducers can be efficiently introduced into the reaction chamber 2 to participate in the reaction. The motion trajectory of the transducer is a two-dimensional horizontal motion, and the trajectory of the motion is perpendicular to the module body 1.

In some embodiments, as shown in fig. 3 and 4, the reaction chamber module comprises a filter chamber 7, the filter chamber 7 is disposed in the gas flow channel 4, and the filter chamber is filled with a filter filler 71 for filtering gas.

In some embodiments, along the flow of the gas flow in the gas flow channel 4, a plurality of enlarged cavities can be provided on the gas flow channel 4 as the filter chambers 7 to be filled with the filter filler 71, so that the air discharged from the reaction chamber 2 or pushed into the reaction chamber 2 can be simply filtered to avoid mutual contamination between the outside air and the reaction system in the reaction chamber 2.

To sum up each embodiment, reaction chamber 2 in this disclosure does benefit to the waste liquid and discharges, and the volume and the number of reaction chamber 2 in module main part 1 all can be adjusted according to actual need, can provide the reaction chamber of large capacity, and for example the volume can reach more than 200uL to its waste liquid of relying on gravity is got rid of the structure and can is avoided the residue of waste liquid. And the energy guide structure 5 on the side surface of the reaction cavity based on the high-efficiency energy conduction can introduce vibration energy into the reaction system so as to improve the uniform mixing efficiency and the sample cracking efficiency.

The specific structure of the reaction chamber module will be described in detail below by way of specific examples of the reaction chamber module.

In this embodiment, the reaction cavity module has the functions of containing and uniformly mixing the reaction system and discharging the reaction waste liquid. The reaction cavity module comprises a substrate 11 and a back plate 12. The substrate 11 is hollowed out from one side to form a cylindrical reaction chamber body, the volume of which is related to the volume of the reaction system. Meanwhile, a flow channel (i.e., a channel for forming the flow channel 3) is led out from a horizontal tangent line at the bottom of the reaction chamber 2, and is opened to the back surface of the substrate 11 (i.e., the first through hole 31) at a position where the flow channel is higher than the apex of the reaction chamber. On the other hand, another gas pressure equilibrium channel (i.e., a channel for forming the gas flow passage 4) is led upward from the apex of the reaction chamber, and the tip of the gas pressure equilibrium channel penetrates the substrate 11 to the back surface to form an opening (i.e., the second through hole 41). The back plate 12 is bonded to the substrate 11 by gluing, ultrasonic bonding, hot pressing, etc. to close the pipeline and the reaction chamber.

In this embodiment, the module body 1 of the chamber module comprises a base plate 11 and a backing plate 12. Wherein, the size of the main body of the substrate 11 is 33mm 23mm 3mm, and the material is PC; the back plate 12 is 33mm 23mm 0.3mm in size and is made of PC.

In this particular embodiment, the reaction chamber 2 is cylindrical and has a dimension of radius R6 mm and an axial dimension h 2 mm. The corresponding flow channel 3 and the flow channel 4 have the width of 0.5mm and the depth of 1 mm.

In this particular embodiment, the first and second through

holes

31 and 41 have dimensions of 0.5mm radius R and 3mm depth.

In the first embodiment, the second through holes 41 are spaced 14mm from the highest point of the top of the reaction chamber 2, and the length of the flow channel section of the flow channel 3 tangent to the bottom of the reaction chamber 2 is 10 mm. The substrate 11 and the back plate 12 are bonded and molded by means of hot pressing, gluing or ultrasonic bonding.

Second embodiment on the basis of the first embodiment, a filter chamber 7 is provided in the middle of the gas flow channel 4. The filter cavity 7 is long-strip-shaped, the two ends of the filter cavity are semi-circles with the radius R being 1.5mm, the middle part of the filter cavity is 5mm long, and the depth of the filter cavity is 2 mm. The size and shape of the filter element matched with the filter element are the same as those of the filter cavity 7, and the filter element can be made of filter paper, sponge, acetate fiber and other fiber braided fabrics with adsorption and filtration functions or a porous structure body consisting of organic high polymers. Optionally, the filter element is made of multi-layer filter paper. And pressing the filter element into the filter cavity 7, and then attaching the substrate 11 and the back plate 12 in a hot pressing mode to complete the assembly of the reaction cavity chip.

And the mixing efficiency of the reagent in the reaction cavity is enhanced by adopting an external energy auxiliary mode. The external energy can be ultrasonic, mechanical vibration, eccentric vibrator, electromagnetic transducer and other mechanical transducers which can complete reciprocating vibration on one plane. An ultrasound probe is preferred.

In the third, fourth and fifth embodiments, in order to enhance the energy introduction of the reaction chamber and reduce the loss in the energy transfer process, the cylindrical, conical and hemispherical energy-conducting structures 5 are respectively introduced on the back surface of the substrate 11. The energy guide structure 5 is positioned at the center of the back surface of the reaction cavity, the radius of the bottom surface is 1.5mm, and the height is 1.0 mm. It acts to increase its energy transfer efficiency by increasing the pressure and simultaneously reducing the contact area of the chip with the transducer.

In the sixth embodiment, the substrate surface of the reaction chamber is set to be a curved surface with a slight curvature as the energy guiding structure 5, the radius of the curved surface is 36.5mm, and the curved surface angle is 9.5 °.

In the seventh embodiment, in order to suppress the reaction system from flowing upward along the edge of the cylindrical reaction chamber during the reaction process by capillary phenomenon and edge effect of the liquid, the upper half of the reaction chamber is modified to be square. The square is tangent with the two sides and the upper edge of the cylindrical reaction cavity, namely the height is 6mm, the length is 12mm, and the depth is 1 mm.

The reaction cavity module comprises an independent large-capacity reaction cavity, can be independently used, and can also be integrated into any microfluidic reaction detection chip to be used as a main or secondary reaction mechanism of the chip.

Some embodiments provide a microfluidic chip including the reaction chamber module described above.

Aiming at the problem that the existing microfluidic detection chip system is difficult to meet the requirement of a large-capacity reaction cavity, the reaction cavity module which is simple in structure and can be quickly formed through die sinking is provided. The reaction cavity in the reaction cavity module can realize seamless butt joint of reaction volumes above 200uL, the efficient reaction system is uniformly mixed, and reaction waste liquid is completely discharged. Meanwhile, the reaction cavity module can be integrated with any reaction chip, and has extremely strong universality; the mold can be opened, and the method has extremely high economy. The application of the reaction cavity module can greatly improve the clinical application value of the microfluidic detection chip.

In some embodiments, the microfluidic chip comprises a device 8 for providing vibrational energy into the reaction chamber 2 in the reaction chamber module. The vibrational energy may be achieved by transducers such as ultrasonic, mechanical vibration, sound waves, and the like.

In some embodiments, the device 8 for providing vibrational energy comprises an ultrasonic transducer, an eccentric vibrator, an electromagnetic transducer.

In the description of the present invention, it should be understood that the terms "first", "second", "third", etc. are used to define the components, and are used only for the convenience of distinguishing the components, and if not otherwise stated, the terms have no special meaning, and thus, should not be construed as limiting the scope of the present invention.

Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present invention and not to limit the same; although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art will understand that: modifications to the specific embodiments of the invention or equivalent substitutions for parts of the technical features may be made; without departing from the spirit of the present invention, it is intended to cover all aspects of the invention as defined by the appended claims.

Claims

1. A reaction chamber module, comprising:

a module body (1);

the reaction cavity (2) is arranged in the module main body (1), and the bottom of the reaction cavity (2) protrudes downwards; the reaction cavity (2) comprises a cylindrical reaction cavity, the radial direction of the cylindrical reaction cavity is the direction between the top and the bottom of the reaction cavity (2), and the axial direction of the cylindrical reaction cavity is the direction between two opposite side parts of the reaction cavity (2);

a liquid flow channel (3) arranged in the module main body (1), wherein a first end of the liquid flow channel is communicated with the reaction cavity (2) through the lowest end of the bottom of the reaction cavity (2), and a second end of the liquid flow channel is communicated with the outside of the module main body (1); the flow channel (3) comprises a channel section tangential to the bottom of the cylindrical reaction chamber; and

and the gas flow channel (4) is arranged in the module main body (1), the first end of the gas flow channel is in gas communication with the reaction cavity (2) through the top of the reaction cavity (2), and the second end of the gas flow channel is in gas communication with the outside of the module main body (1).

2. A reaction chamber module according to claim 1, characterized by comprising an energy-conducting structure (5) arranged outside the module body (1) where the reaction chamber (2) is located for contacting a device (8) for providing vibrational energy into the reaction chamber (2) for transferring energy into the reaction chamber (2).

3. A reactor chamber module according to claim 1, wherein the gas flow channel (4) comprises a channel section tangential to the top of the cylindrical reactor chamber.

4. A reaction chamber module according to claim 1, characterized by comprising a buffer part (6), wherein one end of the buffer part (6) is tangential to the top of the cylindrical reaction chamber, and the other end is communicated with the cylindrical reaction chamber.

5. A reaction chamber module according to claim 1, characterized by comprising an energy conducting structure (5) arranged outside the module body (1) and on the central axis of the cylindrical reaction chamber for contacting a device (8) for providing vibrational energy into the cylindrical reaction chamber for transferring energy into the cylindrical reaction chamber.

6. A reaction chamber module according to claim 2 or 5, characterized in that the energy guiding structure (5) is cylindrical, conical or hemispherical.

7. A reaction chamber module according to claim 2 or 5, wherein the energy guiding structure (5) comprises an outwardly convex wall surface provided at a position corresponding to the reaction chamber (2) on one side of the module body (1).

8. A reaction chamber module according to claim 1, characterized by comprising a filter chamber (7), wherein a plurality of expanded cavities are arranged on the gas flow channel (4) as the filter chamber (7), and the filter chamber (7) is filled with a material for filtering gas.

9. A reaction chamber module according to claim 1, wherein more than one reaction chamber (2) is provided in the module body (1), and each reaction chamber (2) is provided with the flow channel (3) and the gas flow channel (4).

10. A microfluidic chip comprising the reaction chamber module according to any one of claims 1 to 9.

11. Microfluidic chip according to claim 10, comprising a device (8) for providing vibrational energy into the reaction chamber (2) in the reaction chamber module.

12. Microfluidic chip according to claim 11, characterised in that the device (8) for providing vibrational energy comprises an ultrasonic transducer, an eccentric vibrator or an electromagnetic transducer.