CN217568785U

CN217568785U - Micro-fluidic chip

Info

Publication number: CN217568785U
Application number: CN202123351006.8U
Authority: CN
Inventors: 郭永琪; 林宝宝; 邹瑜亮; 杨毅
Original assignee: Beijing Zijing Biotechnology Co ltd
Current assignee: Beijing Zijing Biotechnology Co ltd
Priority date: 2021-12-28
Filing date: 2021-12-28
Publication date: 2022-10-14
Anticipated expiration: 2031-12-28

Abstract

The utility model provides a micro-fluidic chip, including the chip body, the chip body includes chip upper portion and chip bottom, be constructed first cavity on the chip upper portion, the second cavity, be constructed first reaction chamber and second reaction chamber on the chip bottom, the built-in first push rod subassembly that is equipped with of first cavity, be equipped with pressure release subassembly in the second cavity, when exerting the power towards chip bottom one side on first push rod subassembly, fluid in the first cavity can get into first reaction intracavity and get into the second reaction intracavity via the second cavity, pressure release subassembly includes hydrophobic ventilative gasket, in order can be full of the back at the fluid in second reaction chamber, gas and/or liquid in the fluid can get into in the second cavity that pressure release subassembly kept away from chip bottom one side from the pressure release subassembly. The utility model discloses, can make the gas in the transmission fluid discharged to effectively prevent that the bubble in the fluid from producing, thereby effectively improve reaction detection quality, guarantee the accuracy of testing result.

Description

Micro-fluidic chip

Technical Field

The utility model belongs to the technical field of micro-fluidic chip design, concretely relates to micro-fluidic chip.

Background

Microfluidics (microfluidics) is a technology that controls micro-volume fluids to achieve various micro-scale physical, chemical and biological processes. Microfluidics can integrate reactions performed in many laboratories onto microfluidic chips and are therefore referred to as lab-on-a-chip in many scenarios. The volume of fluid processed may also be larger or smaller in some particular cases. Microfluidic chips typically have one or more fluid channels therein. Under the action of different action mechanisms such as external pressure, density, gravity, surface tension, capillary action, mechanical action and the like, fluid can be transmitted in the flow channel of the microfluidic chip.

The micro-fluidic chip can realize biochemical reactions of various different mechanisms and carry out analysis and research on molecules, cells and tissue layers. For example, amplification and detection of a specific nucleic acid sequence can be achieved by Polymerase Chain Reaction (PCR) on a microfluidic chip; for another example, specific proteins can be detected on microfluidic chips by enzyme-linked immunosorbent assay (ELISA). The microfluidic chip may also be used for cell culture to study the response of cells to different biochemical substances. The fluid on the microfluidic chip can be liquid or gas. Most of the existing transmission modes have complex devices, high cost and difficult fluid realization, and the cost and the manufacturing difficulty are also increased for external automatic equipment. In the process of fluid transmission of the microfluidic chip, the liquid fluid may generate bubbles and may not fill the chamber in some cases.

SUMMERY OF THE UTILITY MODEL

Therefore, the to-be-solved technical problem of the present invention is to provide a micro-fluidic chip, which can discharge the gas in the transmission fluid, thereby effectively preventing the bubbles in the fluid from generating, thereby effectively improving the reaction detection quality and ensuring the accuracy of the detection result.

In order to solve the problem, the utility model provides a micro-fluidic chip, which comprises a chip body, the chip body includes chip upper portion and chip bottom, first cavity, second cavity have been constructed on chip upper portion, first reaction chamber and second reaction chamber have been constructed on the chip bottom, the built-in first push rod subassembly that is equipped with of first cavity, be equipped with pressure release subassembly in the second cavity, when applying the orientation the power of chip bottom one side in when on the first push rod subassembly, fluid in the first cavity can get into first reaction intracavity and via the second cavity gets into in the second reaction chamber, pressure release subassembly includes hydrophobic breathable gasket, in order can the fluid in second reaction chamber is full of the back, gas and/or liquid in the fluid can follow pressure release subassembly gets into pressure release subassembly keeps away from chip bottom one side in the second cavity.

In some embodiments, the pressure relief assembly comprises a pressure relief rubber plug, the pressure relief rubber plug is a hollow cylindrical structure, and the hydrophobic air-permeable gasket is cylindrical and is filled in the hollow cylindrical structure.

In some embodiments, an elastic membrane is disposed between the upper chip portion and the bottom chip portion, the elastic membrane has a first check valve, a second check valve, and a first flow through hole, the first flow through hole corresponds to the first chamber, the first check valve and the second check valve correspond to the second chamber, the first check valve only allows one-way fluid flow from the first reaction chamber to the second reaction chamber, and the second check valve only allows one-way fluid flow from the second reaction chamber to the second reaction chamber.

In some embodiments, a hydrophobic membrane is disposed within the first flow-through hole.

In some embodiments, a sample application cavity is further configured on the upper part of the chip, and a third one-way valve is further configured on the elastic membrane, wherein the third one-way valve only allows one-way fluid flow from the sample application cavity to the first reaction cavity.

In some embodiments, a third chamber is further configured on the upper portion of the chip, and a second push rod assembly is disposed in the third chamber, and when a force toward the bottom side of the chip is applied to the second push rod assembly, the fluid in the third chamber can enter the second reaction chamber.

In some embodiments, a fourth chamber is further configured on the upper portion of the chip, a fourth one-way valve is further configured on the elastic membrane, the position of the fourth one-way valve simultaneously corresponds to the fourth chamber, and the fourth one-way valve only allows one-way fluid flow from the second reaction chamber to the fourth chamber; and/or a second flow through hole is formed in the elastic membrane, and a hydrophobic membrane is arranged in the second flow through hole.

In some embodiments, a fifth chamber is further configured on the upper portion of the chip, a fifth one-way valve and a sixth one-way valve are further configured on the elastic film, a communication chamber is further configured on the bottom portion of the chip, the position of the fifth one-way valve corresponds to the fourth chamber, the position of the sixth one-way valve corresponds to the fifth chamber, the fifth one-way valve only allows one-way flow of fluid from the fourth chamber to the communication chamber, and the sixth one-way valve only allows one-way flow of fluid from the communication chamber to the fifth chamber.

In some embodiments, the first push rod assembly and/or the second push rod assembly comprises a push rod and a push rod rubber plug at the tail end of the push rod, and the outer circumferential wall of the push rod rubber plug is in contact sealing with the corresponding chamber.

In some embodiments, the inner side of the push rod rubber plug is embedded with a hard gasket.

The utility model provides a pair of micro-fluidic chip, through first reaction chamber with set up in the second cavity between the second reaction chamber and have the pressure release subassembly of hydrophobic breathable gasket can make the gas in the transmission fluid discharged, thereby effectively prevents bubble in the fluid produces to effectively improve reaction detection quality, guarantee the accuracy of testing result.

Drawings

Fig. 1 is an exploded view of a microfluidic chip according to an embodiment of the present invention;

FIG. 2 is a cross-sectional view of the pressure relief assembly of FIG. 1;

FIG. 3 is a schematic perspective view of the elastic membrane of FIG. 1;

FIG. 4 is a schematic perspective view of the bottom of the chip in FIG. 1;

FIG. 5 is an exploded view of the first pushrod assembly or the second pushrod assembly of FIG. 1;

FIG. 6 is a diagram showing the transfer of fluid (sample and reagent mixture) from the first reaction chamber to the second chamber when the first push-bar assembly is depressed, but the first one-way valve is not yet opened;

FIG. 7 is a diagram showing the state of the fluid (sample and reagent mixed fluid) transferred from the first reaction chamber to the second chamber when the first push rod assembly is pushed down, in which the first check valve is opened and the second check valve is not yet opened, and during this process, the gas in the fluid is exhausted to the side of the second chamber away from the bottom of the chip by the hydrophobic gas-permeable gasket of the pressure relief assembly;

FIG. 8 is a diagram showing the state of fluid (sample and reagent mixed fluid) transfer from the first reaction chamber to the second chamber and the second reaction chamber when the first push rod assembly is pushed down, wherein the first check valve and the second check valve are both opened;

fig. 9 is a diagram illustrating a state that the fluid (the sample and reagent mixed fluid) is transferred from the first reaction chamber to the second chamber and the second reaction chamber when the first push rod assembly is pressed down, at this time, the first check valve and the second check valve are both opened, the second reaction chamber is filled with the fluid, and the fluid pressure is increased and then discharged to a side of the second chamber far away from the bottom of the chip by the hydrophobic air-permeable gasket of the pressure release assembly, so as to realize the quantification of the second reaction chamber;

fig. 10 is a diagram illustrating a state where the fluid (the sample and reagent mixed fluid) is completely transferred from the first reaction chamber to the second reaction chamber when the first push rod assembly is pushed down.

The reference numbers are given as:

11. a chip upper portion; 111. a first chamber; 112. a second chamber; 113. a sample application cavity; 1131. a sealing plug; 114. a third chamber; 115. a fourth chamber; 116. a fifth chamber; 12. the bottom of the chip; 121. a first reaction chamber; 122. a second reaction chamber; 123. a communicating cavity; 21. a first push rod assembly; 22. a second push rod assembly; 23. a push rod; 24. a push rod rubber plug; 25. a hard pad; 3. an elastic film; 31. a first check valve; 32. a second check valve; 33. a first flow through hole; 34. a third check valve; 35. a fourth check valve; 36. a fifth check valve; 37. a sixth check valve; 38. a second flow through hole; 4. a pressure relief assembly; 41. a hydrophobic breathable gasket; 42. and (4) releasing the rubber plug.

Detailed Description

Referring to fig. 1 to 10 in combination, according to an embodiment of the present invention, a microfluidic chip is provided, which includes a chip body, the chip body includes a chip upper portion 11 and a chip bottom portion 12, a first chamber 111 and a second chamber 112 are configured on the chip upper portion 11, a first reaction chamber 121 and a second reaction chamber 122 are configured on the chip bottom portion 12, a first push rod assembly 21 is installed in the first chamber 111, a pressure relief assembly 4 is installed in the second chamber 112, when a force toward one side of the chip bottom portion 12 is applied to the first push rod assembly 21, a fluid in the first chamber 111 can enter the first reaction chamber 121 and enter the second reaction chamber 122 through the second chamber 112, the pressure relief assembly 4 includes a hydrophobic air-permeable gasket 41, so that after the fluid in the second reaction chamber 122 is filled, a gas and/or a liquid in the fluid can enter the second reaction chamber 112 on one side of the pressure relief assembly 4 away from the chip bottom portion 12. In this technical scheme, through set up in the second cavity 112 between first reaction chamber 121 and the second reaction chamber 122 have the pressure release subassembly of hydrophobic gas permeable gasket 41, can make the gas in the transmission fluid be discharged, thereby effectively prevent the bubble in the fluid from producing, thereby effectively improve reaction detection quality, guarantee the accuracy of testing result. It should be noted that, after the pressure of the fluid is increased to a certain value, the hydrophobic effect of the hydrophobic air-permeable gasket 41 is destroyed, and at this time, the fluid (liquid) passes through the hydrophobic air-permeable gasket 41, so as to implement the pressure relief of the liquid fluid, as shown in the states of fig. 9 and 10.

The pressure relief assembly 4 comprises a pressure relief rubber plug 42, the pressure relief rubber plug 42 is of a hollow cylindrical structure, the hydrophobic breathable gasket 41 is filled in the hollow cylindrical structure, the pressure relief rubber plug 42 has large deformability, and can be tightly attached to the inner cavity wall of the second cavity 112 to prevent the fluid below from leaking between the pressure relief rubber plug 42 and the inner cavity wall.

In some embodiments, an elastic film 3 is disposed between the chip upper portion 11 and the chip bottom portion 12, the elastic film 3 is configured with a first one-way valve 31, a second one-way valve 32 and a first through hole 33, the position of the first through hole 33 corresponds to the first chamber 111, the positions of the first one-way valve 31 and the second one-way valve 32 correspond to the second chamber 112, the first one-way valve 31 only allows one-way flow of fluid from the first reaction chamber 121 to the second chamber 112, the second one-way valve 32 only allows one-way flow of fluid from the second chamber 112 to the second reaction chamber 122, and the fluid in the first reaction chamber 121 can be controllably transferred into the second reaction chamber 122 by disposing the first one-way valve 31 and the second one-way valve 32, so that backflow does not occur during the transfer process, and the transfer effect of the fluid is ensured. It should be noted that the water permeation opening pressure of the hydrophobic air-permeable pad 41 should be greater than the opening pressure of the one-way valve on the elastic membrane 3, so as to ensure the hydrophobic air-permeable effect of the hydrophobic air-permeable pad 41.

In some embodiments, the upper portion 11 of the chip is further configured with a sample-adding cavity 113 for detecting addition of a sample, and a cavity opening of the sample-adding cavity is sealed by a sealing plug 1131 after sample addition is completed, and it should be noted that the sealing plug 1131 has a convex sealing column extending into the sample-adding cavity 113, and an extending length of the convex sealing column is large enough to inject the sample therein into the first reaction cavity 121 by using a sealing process, and the elastic membrane 3 is further configured with a third one-way valve 34, where the third one-way valve 34 only allows one-way flow of fluid from the sample-adding cavity 113 to the first reaction cavity 121, and at this time, a sealing push-down action of the sealing plug 1131 can open the third one-way valve 34, so that the sample can enter the first reaction cavity 121 in the sealing process after sample addition, and a corresponding sample-adding or pumping structure does not need to be separately provided, and the structure is simple and compact.

A third chamber 114 is further configured on the chip upper portion 11, a second push rod assembly 22 is installed in the third chamber 114, when a force toward the chip bottom portion 12 side is applied to the second push rod assembly 22, a fluid in the third chamber 114 can enter the second reaction chamber 122, further, a fourth chamber 115 is further configured on the chip upper portion 11, a fourth one-way valve 35 is further configured on the elastic membrane 3, the position of the fourth one-way valve 35 corresponds to the fourth chamber 115 at the same time, the fourth one-way valve 35 allows only one-way flow of the fluid from the second reaction chamber 122 to the fourth chamber 115, further, a fifth chamber 116 is further configured on the chip upper portion 11, a fifth one-way valve 36 and a sixth one-way valve 37 are further configured on the elastic membrane 3, a communication chamber 123 is further configured on the chip bottom portion 12, the position of the fifth one-way valve 36 corresponds to the fourth chamber 115, the position of the sixth one-way valve 37 corresponds to the fifth chamber 116, the position of the fifth one-way valve 37 corresponds to the fifth one-way valve 116, the fifth one-way valve 36 and the sixth one-way valve 123 allows only one-way flow of the fluid from the fourth chamber 115 to the fourth one-way valve 123. Therefore, the chip body is provided with more chambers, so that enough storage space can be provided, sample storage, reaction reagent storage, sample waste liquid storage and recovery after reaction and the like are integrated, and the chambers are matched with the first reaction cavity 121, the second reaction cavity 122 and the communicating cavity 123, so that the whole flow of the whole biological sample detection process is realized. It is worth mentioning that, in the technical scheme, only three steps of pressing-down operations are required to realize 'sample input and result output', specifically, the first step is to adopt the sealing and pressing-down process of the sealing plug 1131 after sample addition is finished to realize that the sample enters the first reaction cavity 121, and the second step is to press down the first push rod assembly 21 to realize that the reagent in the first cavity 111 enters the first reaction cavity 121 and the fluid is transferred from the first reaction cavity 121 to the second reaction cavity 122, so that the effects of transmission without bubbles and quantitative transmission can be realized under the action of the pressure relief assembly 4 in the process; the third step is to depress the second push rod assembly 22 to allow the reagent in the third chamber 114 to enter the second reaction chamber 122, and to be able to enter the fourth chamber 115 and finally the fifth chamber 116.

The elastic membrane 3 is further configured with a second flow hole 38, and hydrophobic membranes are disposed in the first flow hole 33 and the second flow hole 38.

In some embodiments, the first pushing rod assembly 21 and/or the second pushing rod assembly 22 includes a pushing rod 23 and a pushing rod rubber plug 24 at the end (having a clamping groove) of the pushing rod 23, and the outer circumferential wall of the pushing rod rubber plug 24 is in contact with the corresponding chamber for sealing. The inner side of the push rod rubber plug 24 is preferably embedded with a hard gasket 25, so that the push rod rubber plug 24 is stressed uniformly when being stressed, and automatic operation matched with an automatic instrument is realized.

The technical solution of the present invention is further explained with reference to fig. 6 to 10.

The utility model discloses technical scheme's micro-fluidic chip can realize saving and transmission to multiple biological reagent, need add multiple biological substance in proper order in the reaction tube in order to realize different processing and reaction at the biological reaction in-process, give the implementation mode that a common liquid transmitted in proper order here, transmit the reaction liquid of first reaction chamber 121 to second reaction chamber 122, this structure can realize the transmission of more reaction liquids according to the demand, the principle is similar, concrete implementation mode is:

(1) The reaction solution is filled in the first reaction chamber 121, and at this time, the reaction solution is quantitative because the first reaction chamber 121 is filled with the reaction solution. The upper chip part 11 is bonded with the bottom chip part 12, and sealing of the cavity is realized, as shown in fig. 6;

(2) The first push rod assembly 21 is pushed downwards, and the reaction liquid opens the first one-way valve 31 along with the downward pressure, and then comes to the pressure relief assembly 4, as shown in fig. 7;

(3) When the first push rod assembly 21 is pushed downwards, the reaction solution will open the second check valve 32 and be transferred to the second reaction chamber 122, as shown in fig. 8;

(4) Continuing to push the first push rod assembly 21, the reaction solution fills the second reaction chamber 122, at this time, the pressure relief assembly 4 reaches the critical value of the liquid pressure borne by the second reaction chamber, and the excess reaction solution is released to the side of the hydrophobic air-permeable gasket 41 away from the bottom 12 of the chip through the hydrophobic air-permeable gasket 41, as shown in fig. 9;

(5) Continuing to push the first push rod assembly 21 downward, the first push rod assembly 21 pushes to the bottom, and the excess pressure and reaction liquid are completely released to the side of the hydrophobic gas-permeable pad 41 away from the chip bottom 12, as shown in FIG. 10.

It is readily understood by a person skilled in the art that the advantageous ways described above can be freely combined, superimposed without conflict.

The above description is only exemplary of the present invention and should not be construed as limiting the present invention, and any modifications, equivalents and improvements made within the spirit and principles of the present invention are intended to be included within the scope of the present invention. The above is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.

Claims

1. The microfluidic chip is characterized by comprising a chip body, wherein the chip body comprises a chip upper part (11) and a chip bottom part (12), a first cavity (111) and a second cavity (112) are constructed on the chip upper part (11), a first reaction cavity (121) and a second reaction cavity (122) are constructed on the chip bottom part (12), a first push rod assembly (21) is arranged in the first cavity (111), a pressure relief assembly (4) is arranged in the second cavity (112), when a force towards one side of the chip bottom part (12) is exerted on the first push rod assembly (21), fluid in the first cavity (111) can enter the first reaction cavity (121) and enter the second reaction cavity (122) through the second cavity (112), the pressure relief assembly (4) comprises a hydrophobic air-permeable gasket (41), so that after the fluid in the second reaction cavity (122) is filled with the fluid, gas and/or liquid in the fluid can enter the pressure relief assembly (4) from one side of the pressure relief assembly (4), which is far away from the chip bottom part (12) of the chip bottom part (112).

2. The microfluidic chip according to claim 1, wherein the pressure relief assembly (4) comprises a pressure relief rubber plug (42), the pressure relief rubber plug (42) is a hollow cylindrical structure, and the hydrophobic air-permeable gasket (41) is cylindrical and is filled in the hollow cylindrical structure.

3. The microfluidic chip according to claim 1, wherein an elastic membrane (3) is disposed between the chip upper portion (11) and the chip bottom portion (12), the elastic membrane (3) is configured with a first one-way valve (31), a second one-way valve (32) and a first flow through hole (33), the first flow through hole (33) corresponds to the first chamber (111), the first one-way valve (31) and the second one-way valve (32) correspond to the second chamber (112) at the same time, the first one-way valve (31) allows only one-way fluid flow from the first reaction chamber (121) to the second chamber (112), and the second one-way valve (32) allows only one-way fluid flow from the second chamber (112) to the second reaction chamber (122).

4. The microfluidic chip according to claim 3, wherein a hydrophobic membrane is disposed in the first flow through hole (33).

5. The microfluidic chip according to claim 3, wherein a sample application chamber (113) is further configured on the chip upper portion (11), and a third one-way valve (34) is further configured on the elastic membrane (3), wherein the third one-way valve (34) allows only one-way fluid flow from the sample application chamber (113) to the first reaction chamber (121).

6. The microfluidic chip according to claim 5, wherein a third chamber (114) is further formed on the upper chip portion (11), the third chamber (114) houses a second push rod assembly (22), and when a force is applied to the second push rod assembly (22) toward the bottom chip portion (12), a fluid in the third chamber (114) can enter the second reaction chamber (122).

7. The microfluidic chip according to claim 6, wherein a fourth chamber (115) is further configured on the chip upper part (11), a fourth one-way valve (35) is further configured on the elastic membrane (3), the position of the fourth one-way valve (35) simultaneously corresponds to the fourth chamber (115), and the fourth one-way valve (35) only allows one-way fluid flow from the second reaction chamber (122) to the fourth chamber (115); and/or a second flow through hole (38) is formed in the elastic membrane (3), and the second flow through hole (38) is provided with a hydrophobic membrane.

8. The microfluidic chip according to claim 7, wherein a fifth chamber (116) is further configured on the chip upper portion (11), a fifth one-way valve (36) and a sixth one-way valve (37) are further configured on the elastic membrane (3), a communication cavity (123) is further configured on the chip bottom portion (12), the position of the fifth one-way valve (36) corresponds to the fourth chamber (115), the position of the sixth one-way valve (37) corresponds to the fifth chamber (116), the fifth one-way valve (36) allows only one-way flow of fluid from the fourth chamber (115) to the communication cavity (123), and the sixth one-way valve (37) allows only one-way flow of fluid from the communication cavity (123) to the fifth chamber (116).

9. The microfluidic chip according to any of claims 1 to 8, wherein the first push rod assembly (21) and/or the second push rod assembly (22) comprises a push rod (23) and a push rod rubber plug (24) at the end of the push rod (23), and the outer circumferential wall of the push rod rubber plug (24) is in contact sealing with the corresponding chamber.

10. The microfluidic chip according to claim 9, wherein the push rod rubber plug (24) is embedded with a hard gasket (25) on its inner side.