CN110836805A - Preparation method of staining agent test strip for active staining - Google Patents
Preparation method of staining agent test strip for active staining Download PDFInfo
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- CN110836805A CN110836805A CN201911183211.2A CN201911183211A CN110836805A CN 110836805 A CN110836805 A CN 110836805A CN 201911183211 A CN201911183211 A CN 201911183211A CN 110836805 A CN110836805 A CN 110836805A
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- 238000010186 staining Methods 0.000 title claims abstract description 31
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 238000001035 drying Methods 0.000 claims abstract description 43
- 239000000975 dye Substances 0.000 claims abstract description 28
- 238000002791 soaking Methods 0.000 claims abstract description 23
- 239000003086 colorant Substances 0.000 claims abstract description 21
- 239000002131 composite material Substances 0.000 claims abstract description 21
- 239000002904 solvent Substances 0.000 claims abstract description 15
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 14
- 238000004519 manufacturing process Methods 0.000 claims abstract description 14
- 230000002194 synthesizing effect Effects 0.000 claims abstract description 14
- 238000003825 pressing Methods 0.000 claims abstract description 8
- 238000002156 mixing Methods 0.000 claims abstract description 3
- 238000004043 dyeing Methods 0.000 claims description 40
- 238000000034 method Methods 0.000 claims description 20
- 239000007788 liquid Substances 0.000 claims description 18
- 241000282376 Panthera tigris Species 0.000 claims description 17
- KZMRYBLIGYQPPP-UHFFFAOYSA-M 3-[[4-[(2-chlorophenyl)-[4-[ethyl-[(3-sulfonatophenyl)methyl]azaniumylidene]cyclohexa-2,5-dien-1-ylidene]methyl]-n-ethylanilino]methyl]benzenesulfonate Chemical compound C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C(=CC=CC=2)Cl)C=CC=1N(CC)CC1=CC=CC(S([O-])(=O)=O)=C1 KZMRYBLIGYQPPP-UHFFFAOYSA-M 0.000 claims description 13
- 230000015572 biosynthetic process Effects 0.000 claims description 12
- 238000003786 synthesis reaction Methods 0.000 claims description 12
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 claims description 10
- 229960000907 methylthioninium chloride Drugs 0.000 claims description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
- 239000008213 purified water Substances 0.000 claims description 8
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 claims description 6
- 239000012128 staining reagent Substances 0.000 claims description 6
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims description 5
- 229940020947 fluorescein sodium Drugs 0.000 claims description 5
- 238000001914 filtration Methods 0.000 abstract description 8
- 238000003756 stirring Methods 0.000 abstract description 8
- 239000000243 solution Substances 0.000 description 29
- 210000004027 cell Anatomy 0.000 description 9
- 238000005520 cutting process Methods 0.000 description 5
- 229910001220 stainless steel Inorganic materials 0.000 description 5
- 239000010935 stainless steel Substances 0.000 description 5
- 238000005303 weighing Methods 0.000 description 5
- 239000011259 mixed solution Substances 0.000 description 4
- 230000005540 biological transmission Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 125000001434 methanylylidene group Chemical group [H]C#[*] 0.000 description 3
- SGHZXLIDFTYFHQ-UHFFFAOYSA-L Brilliant Blue Chemical compound [Na+].[Na+].C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C(=CC=CC=2)S([O-])(=O)=O)C=CC=1N(CC)CC1=CC=CC(S([O-])(=O)=O)=C1 SGHZXLIDFTYFHQ-UHFFFAOYSA-L 0.000 description 2
- 238000009455 aseptic packaging Methods 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 239000000981 basic dye Substances 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 210000000795 conjunctiva Anatomy 0.000 description 1
- 210000004087 cornea Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- NJDNXYGOVLYJHP-UHFFFAOYSA-L disodium;2-(3-oxido-6-oxoxanthen-9-yl)benzoate Chemical compound [Na+].[Na+].[O-]C(=O)C1=CC=CC=C1C1=C2C=CC(=O)C=C2OC2=CC([O-])=CC=C21 NJDNXYGOVLYJHP-UHFFFAOYSA-L 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 231100001231 less toxic Toxicity 0.000 description 1
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- 239000000047 product Substances 0.000 description 1
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- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention provides a preparation method of a staining agent test strip for active staining, which comprises the following steps: putting the coloring agent and the solvent into a container according to the mass ratio of 1:100-150, mixing, stirring until the coloring agent and the solvent are completely dissolved mutually, and filtering to obtain a coloring agent solution; soaking with filter paper: soaking the cut filter paper in a coloring agent solution for 5-60 seconds; drying by using filter paper: taking out the soaked filter paper, drying at the temperature of 30 +/-5 ℃ for 2-6 hours, and taking down for later use; synthesizing a test strip: and pressing and synthesizing the dried filter paper and the composite paper on a synthesizer to obtain the dyeing agent test strip. The preparation method has the advantages of simple preparation process, high production efficiency, low production cost and convenience for mass production.
Description
Technical Field
The invention relates to the technical field of biomedical treatment, in particular to a preparation method of a staining agent test strip for active staining.
Background
Vital staining is the use of some non-toxic or less toxic dyes to reveal certain natural structures in the cell without affecting the vital functions of the cell or producing any physical or chemical changes that cause cell death. The principle is that general biological materials cannot penetrate cell membranes, and only when the cell membranes are destroyed after the cell is fixed, the dye can enter the cell. However, some dyes (called "vital dyes") enter living cells and are non-toxic or minimally toxic staining agents that stain certain specific structures in the cell. Vital dyes do not substantially or rarely affect the vital activity of the cell. Most of the vital dyes are basic dyes, such as fluorescein sodium, trypan blue, methine blue, lissamine green, tiger red, etc., which are positively charged after dissociation, and some of the dyes are specifically bonded with some intracellular structures.
When the living body dyeing is carried out in the prior art, a user directly mixes the dyeing agent with the required solvent, the dyeing agent is manually prepared during each use, the concentration and the precision of each preparation are not the same, frequent manual operation easily causes the instability of the concentration of the dyeing agent, the quality of the solution is unstable, and the manual operation cost is high.
Disclosure of Invention
In view of the above problems, the present invention is to provide a method for preparing a test strip of a staining agent for reactive staining, which is manufactured according to the needs of a user, and when in use, the test strip of the staining agent is only required to be dipped in a required solvent to stain a tissue to be stained.
In order to achieve the purpose, the invention provides the following technical scheme:
a preparation method of a staining agent test strip for active staining comprises the following steps:
s1, preparing liquid: putting the coloring agent and the solvent into a container according to the mass ratio of 1:100-150, mixing, stirring until the coloring agent and the solvent are completely dissolved mutually, and filtering to obtain a coloring agent solution;
s2, soaking in filter paper: soaking the cut filter paper in a coloring agent solution for 5-60 seconds;
s3, drying by filter paper: taking out the soaked filter paper, drying at the temperature of 30 +/-5 ℃ for 2-6 hours, and taking down for later use;
s4, synthesizing a test strip: and pressing and synthesizing the dried filter paper and the composite paper on a synthesizer to obtain the dyeing agent test strip, wherein the temperature of the synthesizer is set to be 150-280 ℃.
Further, the staining agent comprises at least one of fluorescein sodium, methylene blue, trypan blue, tiger red and lissamine green.
Further, the solvent includes at least one of purified water, methanol, and ethanol.
Further, the length of the staining reagent strip is 20-100cm, and the width of the staining reagent strip is 3-10 cm.
Further, in the filter paper soaking process of S2, a filter paper dyeing device is adopted, and the cut filter paper is placed into a dyeing agent solution in the filter paper dyeing device for soaking;
the filter paper dyeing device comprises a frame body, a conveying mechanism and a dyeing tank are arranged on the frame body, the conveying mechanism comprises two conveying wheels driven by a stepping motor, a conveying chain moving in the horizontal direction is wound on the conveying wheels, the dyeing tank is located below the conveying chain, and the filter paper to be dyed fixed on the conveying chain can be immersed in the dyeing tank in the dyeing solution.
Furthermore, an overflow hole is formed in the wall of the dyeing tank and connected with the liquid inlet end of the solution circulating pipeline, and the liquid outlet end of the solution circulating pipeline is communicated with the liquid inlet of the dyeing tank.
Further, still include lift control platform, lift control platform includes the lift control motor, the output of lift control motor is connected the operation panel for will wait to dye filter paper and transport to through the operation panel the conveying chain below, make and wait to dye filter paper and fix on the conveying chain.
Further, the filter paper to be dyed is fixedly connected to the conveying chain through a filter paper fixing piece, and the filter paper fixing piece is a hook or a clamp or a magnet.
Further, the conveying wheel is driven by a stepping motor.
Further, in the process of synthesizing the test paper strip of S4, the composite paper is firstly folded in half from the middle, and then the dried filter paper is sandwiched between the composite paper and placed in a synthesizer for press synthesis.
Further comprising S5, cutting the staining reagent strip prepared in S4 to a standard size, and then carrying out aseptic packaging.
According to the preparation method of the staining agent test strip for active staining, the obtained staining agent test strip replaces manual frequent operation to prepare a solution, and is convenient and fast to use; in addition, the preparation production process is simple, the production efficiency is high, the production cost is low, and the large-scale production is convenient.
In addition, the filter paper dyeing device adopted in the filter paper infiltration process is simple in structure, is specially used for dyeing filter paper, effectively improves the controllability of the dye for dyeing the filter paper, improves the uniformity of the fuel content in the filter paper, ensures the stable quality of products, and effectively improves the working efficiency.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a process flow diagram of a preparation method of the present invention;
FIG. 2 is a schematic view of the structure of the filter paper dyeing apparatus of the present invention;
FIG. 3 is a schematic view showing a state in which the filter paper of the present invention is immersed in a staining bath.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it is to be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-3, a method for preparing a test strip of a staining agent for reactive staining according to the present invention is illustrated, which comprises the following steps:
s1, preparing liquid: a certain volume of container is used, and a coloring agent and a solvent are put into the container, wherein the mass ratio of the coloring agent to the solvent is 1:100-150, and the coloring agent can be sodium fluorescein, methylene blue, trypan blue, tiger red, lissamine green and the like. The solvent is purified water or methanol, ethanol or other solvents. Stirring with a stirrer, filtering with a disposable filter to obtain a coloring agent solution when the coloring agent and the solvent are completely mutually soluble. The prepared staining agent solution is poured into a stainless steel container.
Preferably, the mass ratio of the coloring agent to the solvent is 1: 100.
S2, soaking in filter paper: to facilitate the dyeing of the filter paper, the filter paper is cut into pieces of filter paper of a suitable size with a paper cutter. And clamping the cut filter paper by using cleaned clamps respectively, wherein the positions of the clamps for clamping the filter paper are 2-5 mm, and soaking the filter paper in a coloring agent solution for 5-60 seconds.
Wherein, a filter paper dyeing device is adopted, and the cut filter paper is put into a dyeing agent solution in the filter paper dyeing device for soaking, as shown in figures 2 and 3;
the filter paper dyeing device comprises a frame body 6, a conveying mechanism and a dyeing tank 1 are arranged on the frame body 6, the conveying mechanism comprises two conveying wheels 5, conveying chains 2 moving in the horizontal direction are wound on the conveying wheels 5, the dyeing tank 1 is located below the conveying chains 2, and the filter paper to be dyed fixed on the conveying chains 2 can be immersed in dye solution in the dyeing tank 1. Can fix a plurality of filter papers on transfer chain 2, make filter paper soak in the dyestuff of dyeing vessel 1, accomplish the dyeing technology of filter paper along with the transmission of transfer chain 2, at last through the manual work with the filter paper after dyeing take off from transfer chain 2 can, the structure setting of this technology makes filter paper be in the same and stable dip-dyeing degree of depth all the time, keeps fixed and stable dip-dyeing speed, finally obtains the filter paper that the dyestuff content is the same.
In order to keep the dye in the dyeing tank 1 uniform all the time and keep the liquid level of the dye stable, and simultaneously immerse the filter paper into the dye at the same depth, the dye is in a circular flowing state through a solution circulating pipeline, an overflow hole 101 is arranged on the tank wall of the dyeing tank 1, as shown in fig. 3; the overflow hole 101 is connected with the liquid inlet end of the solution circulating pipeline, and the liquid outlet end of the solution circulating pipeline is communicated with the liquid inlet of the dyeing tank. The circulation structure is not limited to the overflow hole type, and the overflow groove type and other modes can be adopted.
Still include lifting control platform, lifting control platform includes lifting control motor 7, lifting control motor's output is connected operation panel 9 for will wait to dye filter paper and transport to through operation panel 9 conveying chain 2 below makes and waits to dye filter paper and fix on conveying chain 2.
Set up lifting control platform, the material loading of dyeing process of being convenient for, in this step, the accessible is artifical to be treated the dyed filter paper and is put on operation panel 9, and operation panel 9 rises under the drive of motor, adopts artifical or other mechanical means again to fix filter paper on transfer chain 2. The lifting control process of the lifting control motor 7 can be realized by adopting a lifting technology in the prior art, and the details are not repeated herein.
The filter paper to be dyed is fixedly connected to the conveying chain 2 through the filter paper fixing piece 3, the filter paper fixing piece 3 is a hook, a clamp or a magnet, the filter paper can be hung on the conveying chain 2 through the hook, or the filter paper is clamped by two magnets in a magnet attracting mode, and then the magnet is attracted to the conveying chain 2.
The transmission wheel 5 is driven by a stepping motor 8, and parameters such as the rotating speed of the transmission wheel 5 can be adjusted according to process requirements.
S3, drying by filter paper: and taking out after soaking, putting the mixture into a drying chamber for drying, controlling the drying temperature to be 30 +/-5 ℃, drying for 2-6 hours, and taking down for later use.
S4, synthesizing a test strip: and pressing the dried filter paper with the coloring agent and the folded composite paper on a synthesizer to be integrated. During synthesis, the length of the filter paper of the coloring agent is consistent with that of the folded composite paper. Setting the temperature of the synthesizer to be 150-280 ℃, clamping the dyed filter paper strip in the middle of the folded flat paper, placing the dyed filter paper strip at the appointed position of the synthesizer, aligning two sides of the combined filter paper strip with the baffles at two ends respectively, and starting a switch to complete synthesis.
S5, cutting the staining reagent strip prepared in the S4 to a standard size, and then carrying out aseptic packaging.
Example 1
Preparing the tiger red test strip:
s1, preparing liquid: weighing 25g of tiger red by an electronic balance, placing the tiger red in a stainless steel barrel, adding 2750g of purified water, stirring and filtering to obtain a tiger red solution.
S2, soaking in filter paper: the filter paper was cut into a size of 165mm by 35mm in length by a cutter. Clamping the cut filter paper (length multiplied by width: 165mm multiplied by 35mm) by a cleaned clamp, wherein the position for clamping the filter paper by the clamp is about 2 mm; taking the prepared tiger red solution out of the charging bucket by using a stainless steel spoon and placing the solution into a stainless steel container.
The clamped cut filter paper is soaked in the prepared tiger red solution for 5 seconds (in a stainless steel container), so that the cut filter paper completely absorbs the tiger red solution.
S3, drying by filter paper: taking out the soaked tiger red filter paper, and hanging the tiger red filter paper on a drying rack in a drying chamber for drying. And connecting a fan power supply, turning on a drying fan switch, pressing a heating button, and selecting the gear as an automatic gear. The temperature is controlled at 25-30 ℃, and the drying time is 4 hours until the drying is complete.
S4, synthesis: folding the composite paper (length multiplied by width: 165mm multiplied by 74mm) into halves to prepare a semi-finished product of 165mm multiplied by 37mm for later use.
A synthesizer: the machine adopts 220V single-phase alternating current power supply and is provided with a numerical control thermometer, and equipment is preheated before use.
According to the working condition of the operation, the height of the pedal of the synthesizer is adjusted according to the proper operation.
Firstly, a power supply is plugged, the synthesis temperature is set to be 185-200 ℃, preheating is carried out for 30 minutes, and the synthesis operation can be started when the temperature reaches the set value.
An operator puts the composite white paper well, then sends the tiger red filter paper to a front baffle plate to be tightly leaned on, steps on a foot switch, can be loosened in about 3-4 seconds, and takes out the synthesized tiger red filter paper.
S5, manufacturing a standard test strip: cutting the tiger red filter paper into test strips with the width of 7-8cm and the length of 60cm by using a paper cutter.
The tiger red test strip obtained by the embodiment can be used for cornea and conjunctiva staining.
Example 2
Manufacturing method of methine blue test strip
S1, preparing liquid: weighing 8g of methylene blue by using an electronic balance, placing the methylene blue into a container, adding 1200g of purified water or a mixed solution of water and ethanol, stirring and filtering to obtain a methylene blue solution.
S2, soaking in filter paper: the filter paper was cut into a size of 100mm by 25mm in length by width with a cutter. Clamping the cut filter paper (length multiplied by width: 100mm multiplied by 25mm) by a cleaned clamp, wherein the position for clamping the filter paper by the clamp is about 3 mm; soaking the clamped cut filter paper in the prepared methylene blue solution for 10-15 seconds, so that the cut filter paper can completely absorb the solution.
S3, drying by filter paper: and taking out the soaked methylene blue filter paper, and hanging the filter paper on a drying rack in a drying chamber for drying. And connecting a fan power supply, turning on a drying fan switch, pressing a heating button, and selecting the gear as an automatic gear. The temperature is controlled at 30 ℃, and the drying time is 2-6 hours until the drying is complete.
S4, synthesis: folding the composite paper for synthesis (length multiplied by width: 100mm multiplied by 54mm) in half, starting a synthesizer, wherein the temperature of the synthesizer is 170-230 ℃; and synthesizing the folded composite paper and methylene blue filter paper.
S5, manufacturing a standard test strip: the above methylene blue filter paper was cut into test strips of 6cm in width and 50cm in length with a paper cutter.
The methine blue test strip obtained in the embodiment can be used for staining live cells of yeast.
Example 3
Production of lissamine green test paper strip
S1, preparing liquid: weighing 1g of lissamine green by using an electronic balance, placing in a container, adding 100g of purified water, stirring and filtering to obtain a lissamine green solution.
S2, soaking in filter paper: the filter paper was cut to a length × width of 156mm × 65 mm. Clamping the cut filter paper by a clamp, wherein the position for clamping the filter paper by the clamp is about 3 mm; and soaking the clamped filter paper in the prepared lissamine green solution for 9-15 seconds.
S3, drying by filter paper: and taking out the soaked lissamine green filter paper, and hanging the filter paper on a drying rack in a drying chamber for drying. And starting a drying fan, controlling the temperature to be 25-30 ℃, and drying for 3-5 hours.
S4, synthesis: folding the paper with composite paper (length multiplied by width: 156mm multiplied by 58mm) in half, starting the synthesizer, and synthesizing the folded composite paper with lissamine green filter paper.
S5, manufacturing a standard test strip: the above hyposerine green filter paper was cut into test strips of 6cm and 80cm in length with a paper cutter.
The lissamine green test strip obtained in the embodiment can be used for living cell staining.
Example 4
Preparation of Mixed test strip 1
S1, preparing liquid: weighing 40g of fluorescein sodium and 80g of lissamine green by an electronic balance, placing the weighed materials in a container, adding 12000g of purified water, stirring and filtering to obtain a solution of fluorescein sodium and lissamine green.
S2, soaking in filter paper: the filter paper was cut to have a length × width of 180mm × 60 mm. Clamping the cut filter paper by a clamp, wherein the position for clamping the filter paper by the clamp is about 3 mm; and soaking the clamped filter paper in the prepared mixed solution for 10-20 seconds. The mixed filter paper strip 1 is manufactured.
S3, drying by filter paper: taking out the soaked mixed filter paper 1, and hanging the mixed filter paper on a drying rack in a drying chamber for drying. And starting a drying fan, controlling the temperature to be 25-30 ℃, and drying for 3-4 hours.
S4, synthesis: folding the paper with composite paper (length multiplied by width: 180mm multiplied by 50mm) in half, starting the synthesizer, and synthesizing the folded composite paper with mixed filter paper.
S5, manufacturing a standard test strip: and cutting the mixed filter paper strip 1 into test paper strips with the length of 70cm and the length of 7cm by using a paper cutter.
The mixed test strip 1 obtained in this example can be used for staining the ocular surface of a living body.
Example 5
Preparation of Mixed test strip 2
S1, preparing liquid: weighing 0.5g of trypan blue and 0.3g of brilliant blue by using an electronic balance, placing the weighed materials in a container, adding 120g of purified water, stirring and filtering to obtain a mixed solution of the trypan blue and the brilliant blue.
S2, soaking in filter paper: the filter paper was cut to a length X width of 150mm X50 mm. Clamping the cut filter paper by a clamp, wherein the position for clamping the filter paper by the clamp is about 3 mm; and soaking the clamped filter paper in the prepared mixed solution for 8-10 seconds. The mixed filter paper strip 2 is manufactured.
S3, drying by filter paper: and taking out the soaked mixed filter paper 2, and hanging the mixed filter paper on a drying rack in a drying chamber for drying. And starting a drying fan, controlling the temperature to be 25-30 ℃, and drying for 2-3 hours.
S4, synthesis: folding the paper with composite paper (length multiplied by width: 150mm multiplied by 50mm) in half, starting the synthesizer, and synthesizing the folded composite paper with mixed filter paper.
S5, manufacturing a standard test strip: and cutting the mixed filter paper strip 2 into test paper strips with the length of 60cm and the length of 5cm by using a paper cutter.
The mixed test strip 2 obtained in the embodiment can be used for fundus staining of a living body.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (10)
1. A preparation method of a staining agent test strip for active staining is characterized by comprising the following steps: the method comprises the following steps:
s1, preparing liquid: mixing a coloring agent and a solvent according to a mass ratio of 1:100-150 to obtain a coloring agent solution;
s2, soaking in filter paper: soaking the filter paper in a coloring agent solution for 5-60 seconds;
s3, drying by filter paper: taking out the soaked filter paper, and drying the filter paper for 2-6 hours at the temperature of 30 +/-5 ℃;
s4, synthesizing a test strip: and pressing and synthesizing the dried filter paper and the composite paper to obtain the dyeing agent test strip.
2. The method of claim 1, wherein: the staining agent comprises at least one of fluorescein sodium, methylene blue, trypan blue, tiger red and lissamine green.
3. The method of claim 1, wherein: the solvent comprises at least one of purified water, methanol and ethanol.
4. The method of claim 1, wherein: the length of the staining reagent strip is 20-100cm, and the width of the staining reagent strip is 3-10 cm.
5. The method of claim 1, wherein: and S4, pressing and synthesizing the dried filter paper and the composite paper on a synthesizer to obtain the test paper strip for the coloring agent, wherein the temperature of the synthesizer is set to be 150-280 ℃.
6. The method of claim 1, wherein: in the filter paper soaking process of S2, a filter paper dyeing device is adopted, and the cut filter paper is placed into a dyeing agent solution in the filter paper dyeing device for soaking;
the filter paper dyeing device comprises a frame body, a conveying mechanism and a dyeing tank are arranged on the frame body, the conveying mechanism comprises two conveying wheels driven by a stepping motor, a conveying chain moving in the horizontal direction is wound on the conveying wheels, the dyeing tank is located below the conveying chain, and the filter paper to be dyed fixed on the conveying chain can be immersed in the dyeing tank in the dyeing solution.
7. The method of claim 6, wherein: and the wall of the dyeing tank is provided with an overflow hole, the overflow hole is connected with the liquid inlet end of the solution circulating pipeline, and the liquid outlet end of the solution circulating pipeline is communicated with the liquid inlet of the dyeing tank.
8. The production method according to claim 6 or 7, characterized in that: the filter paper dyeing device further comprises a lifting control platform, the lifting control platform comprises a lifting control motor, the output end of the lifting control motor is connected with the operation platform and used for conveying the filter paper to be dyed to the lower portion of the conveying chain through the operation platform, and the filter paper to be dyed is fixed on the conveying chain.
9. The method of claim 8, wherein: the filter paper to be dyed is fixedly connected to the conveying chain through a filter paper fixing piece which is a hook, a clamp or a magnet.
10. The method of claim 1, wherein: in the process of synthesizing the test paper strip of S4, the composite paper is firstly folded from the middle, then the dried filter paper is clamped in the middle of the composite paper, and the composite paper strip is placed in a synthesizer for pressing synthesis.
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