CN103301478A - Novel mixed type cornea and conjuctiva intravital staining agent - Google Patents
Novel mixed type cornea and conjuctiva intravital staining agent Download PDFInfo
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- CN103301478A CN103301478A CN2012100661908A CN201210066190A CN103301478A CN 103301478 A CN103301478 A CN 103301478A CN 2012100661908 A CN2012100661908 A CN 2012100661908A CN 201210066190 A CN201210066190 A CN 201210066190A CN 103301478 A CN103301478 A CN 103301478A
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- aqueous solution
- fluorescein sodium
- lissamine green
- stain
- preparation
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- 210000004087 cornea Anatomy 0.000 title claims abstract description 11
- 238000010186 staining Methods 0.000 title abstract description 9
- 239000007864 aqueous solution Substances 0.000 claims abstract description 40
- KZMRYBLIGYQPPP-UHFFFAOYSA-M 3-[[4-[(2-chlorophenyl)-[4-[ethyl-[(3-sulfonatophenyl)methyl]azaniumylidene]cyclohexa-2,5-dien-1-ylidene]methyl]-n-ethylanilino]methyl]benzenesulfonate Chemical compound C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C(=CC=CC=2)Cl)C=CC=1N(CC)CC1=CC=CC(S([O-])(=O)=O)=C1 KZMRYBLIGYQPPP-UHFFFAOYSA-M 0.000 claims abstract description 39
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims abstract description 35
- 229940020947 fluorescein sodium Drugs 0.000 claims abstract description 35
- 239000011259 mixed solution Substances 0.000 claims abstract description 21
- 238000002360 preparation method Methods 0.000 claims description 17
- 239000000872 buffer Substances 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000008215 water for injection Substances 0.000 claims description 12
- 239000000975 dye Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 10
- 238000005303 weighing Methods 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 5
- 238000011010 flushing procedure Methods 0.000 claims description 4
- 238000003973 irrigation Methods 0.000 claims description 4
- 230000002262 irrigation Effects 0.000 claims description 4
- 239000008363 phosphate buffer Substances 0.000 claims description 4
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 claims description 2
- 150000001642 boronic acid derivatives Chemical group 0.000 claims description 2
- 239000003153 chemical reaction reagent Substances 0.000 claims description 2
- 210000000795 conjunctiva Anatomy 0.000 abstract description 18
- 206010023332 keratitis Diseases 0.000 abstract description 3
- 239000003795 chemical substances by application Substances 0.000 abstract 5
- 201000010666 keratoconjunctivitis Diseases 0.000 abstract 2
- 208000003556 Dry Eye Syndromes Diseases 0.000 abstract 1
- 206010013774 Dry eye Diseases 0.000 abstract 1
- XQFRJNBWHJMXHO-RRKCRQDMSA-N IDUR Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(I)=C1 XQFRJNBWHJMXHO-RRKCRQDMSA-N 0.000 abstract 1
- 208000021386 Sjogren Syndrome Diseases 0.000 abstract 1
- 210000003560 epithelium corneal Anatomy 0.000 abstract 1
- 210000001519 tissue Anatomy 0.000 abstract 1
- 238000004043 dyeing Methods 0.000 description 18
- 210000002919 epithelial cell Anatomy 0.000 description 6
- 241000282376 Panthera tigris Species 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- 230000006378 damage Effects 0.000 description 3
- 238000012856 packing Methods 0.000 description 3
- 206010011409 Cross infection Diseases 0.000 description 2
- 206010029803 Nosocomial infection Diseases 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000005779 cell damage Effects 0.000 description 2
- 208000037887 cell injury Diseases 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 206010010984 Corneal abrasion Diseases 0.000 description 1
- 208000028006 Corneal injury Diseases 0.000 description 1
- OTMSDBZUPAUEDD-UHFFFAOYSA-N Ethane Chemical compound CC OTMSDBZUPAUEDD-UHFFFAOYSA-N 0.000 description 1
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- 206010064996 Ulcerative keratitis Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 201000007717 corneal ulcer Diseases 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 210000004692 intercellular junction Anatomy 0.000 description 1
- 231100001083 no cytotoxicity Toxicity 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 208000005494 xerophthalmia Diseases 0.000 description 1
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- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
The invention relates to a novel mixed type cornea and conjuctiva intravital staining agent. A staining agent comprises a mixed solution of a fluorescein sodium aqueous solution and a lissamine green aqueous solution. The mixed type cornea and conjuctiva intravital staining agent has the advantages that the staining agent can stain cornea and bulbar conjunctiva, stains at one time, has small stimulus to eye tissues and is easily accepted by patients; the staining agent is mainly used for early diagnosing and evaluating dry eye syndrome, keratohelcosis, keratoconjunctivitis (KCS), arborization corneal epithelium herpes, Sjogren syndrome and the like.
Description
Technical field
The present invention relates to angle conjunctiva vital stain, be specifically related to a kind of new mixed type angle conjunctiva vital stain.Relate in particular to and a kind ofly can directly carry out the dyeing of cornea, conjunctiva, be used for clinically diagnosis cornea, the damage of conjunctiva or the stain of pathological changes, stain can infiltrate the form that be prepared into test strips by filter paper.
Background technology
Eye table dyeing is the convenient and effective method of diagnosis eye surface diseases (especially xerophthalmia cause the damage of eye table).The dyeing of eye table is for assessment of the integrity of cornea, conjunctival epithelial cell, because dyeing can show that epithelial cell and cell junction sustain damage.Fluorescein sodium, red, the lissamine green of tiger are eye table vital stains commonly used.Fluorescein sodium is primary corneal dyeing agent, is mainly used in checking the corneal epithelial cell damage, such as corneal ulcer, corneal abrasion, keratitis etc.Before the discovery lissamine green, Hu Hongze is primary conjunctiva stain, and the cell of the death of main dyeing eye table epithelial cell or decay also can dye when tear film rete malpighii is destroyed.The tiger red colouring has zest, makes patient's tingle, and research also shows the brave red cytotoxicity that has.Norn in 1973 report lissamine green have tiger reddish tint like Color, and the lissamine green zest is little, no cytotoxicity.Lissamine green dyeing tissue is aeruginous, and more brave red tissue staining takes on a red color and is easy to differentiate, and since nineteen ninety-four lissamine green is widely used in clinically gradually, is considered at present desirable conjunctiva stain.The red in recent years then less use of tiger.
Present eye table stain lissamine green and fluorescein sodium are independent use, carry out respectively the dyeing of corneal dyeing and conjunctiva, can't check simultaneously the cell of corneal epithelial cell damage and the death of eye table epithelial cell or decay, twice detection will have certain time interval.Be closely to link to each other at human eye table cornea tissue and conjunctival tissue, dyeing has interference unavoidably respectively, therefore, has the needs that simultaneously above-mentioned symptom checked.
Summary of the invention
The object of the present invention is to provide a kind of new mixed type angle conjunctiva vital stain.
Realize that technical scheme of the present invention is: a kind of new mixed type angle conjunctiva vital stain, described stain comprises the mixed solution of fluorescein sodium aqueous solution and lissamine green aqueous solution.
Preferably, in described fluorescein sodium aqueous solution, the concentration of fluorescein sodium is 0.5%-4.0%w/v.
Preferably, in described lissamine green aqueous solution, the concentration of lissamine green is 0.5%-4.0%w/v.
More preferably, in described fluorescein sodium aqueous solution, the concentration of fluorescein sodium is 0.5%-2.0%w/v.
More preferably, in described lissamine green aqueous solution, the concentration of lissamine green is 0.5%-2.0%w/v.
Preferably, in described mixed solution, the volume ratio of described fluorescein sodium aqueous solution and described lissamine green aqueous solution is 1: 0.25-1: 3.
Preferably, wherein said aqueous solution is physiological buffer.
More preferably, described physiological buffer is borate buffer, phosphate buffer or intraocular irrigation flushing liquor.
A kind of method for preparing described stain said method comprising the steps of:
(1) takes by weighing fluorescein sodium and water for injection, preparation fluorescein sodium aqueous solution;
(2) take by weighing lissamine green and water for injection, preparation lissamine green aqueous solution;
(3) prepare in proportion the mixed solution of fluorescein sodium aqueous solution and lissamine green aqueous solution;
(4) with the prepared mixed solution of filter filtration step (3).
Preferably, the aperture of filter is 0.22u described in the step (4).
Preferably, described method also comprises the water for injection in step (1) and the step (2) is mixed with phosphate buffer, borate buffer or intraocular irrigation flushing liquor in advance.
Preferably, described method comprises that also infiltrating reagent paper with described mixed solution makes the dyestuff test paper bar.
Preferably, described dyestuff test paper bar infiltrates with physiological buffer before use, is attached to gently the eye table and carries out eye table cornea, conjunctiva dyeing.
Compared with prior art, the present invention has following beneficial effect: mixed type of the present invention angle conjunctiva vital stain can disposablely be finished corneal dyeing and conjunctiva dyeing, has shortened Diagnostic Time, and little to ocular tissue's zest, and the patient is easy to accept; Mixed type of the present invention angle conjunctiva vital stain can infiltrate through filter paper and be prepared into the mixed-type ocular surface staining test paper bar, after sterilization, adopts disposable sterilized packing, disposable use, avoid cross infection, improved clinical diagnosis efficient, saved simultaneously cost.
The specific embodiment
Further specify by the following examples and explain the present invention, but not as restriction of the present invention.The included embodiment of this paper more completely understands invention as herein described in order to help.These embodiment limit as herein described or this paper scope required for protection never in any form.
Embodiment 1
(1) take by weighing the physiological buffer that fluorescein sodium and water for injection are prepared, preparation concentration is 0.5% fluorescein sodium aqueous solution;
(2) take by weighing the physiological buffer that lissamine green and water for injection are prepared, preparation concentration is 0.5% lissamine green aqueous solution;
(3) press volume ratio preparation in 1: the 0.25 fluorescein sodium aqueous solution of fluorescein sodium and lissamine green and the mixed solution of lissamine green aqueous solution;
(4) be the prepared mixed solution of 0.22u filter filtration step (3) with the aperture.
(5) infiltrate filter paper with the prepared mixed solution of step (4), make the dyestuff test paper bar, packing, sterilization is for disposable.
Embodiment 2
(1) take by weighing fluorescein sodium and water for injection, preparation concentration is 2.0% fluorescein sodium aqueous solution;
(2) take by weighing lissamine green and water for injection, preparation concentration is 2.0% lissamine green aqueous solution;
(3) press volume ratio preparation in 1: the 1.5 fluorescein sodium aqueous solution of fluorescein sodium and lissamine green and the mixed solution of lissamine green aqueous solution;
(4) be the prepared mixed solution of 0.22u filter filtration step (3) with the aperture.
(5) infiltrate filter paper with the prepared mixed solution of step (4), make the dyestuff test paper bar, packing, sterilization is for disposable.
Embodiment 3
(1) take by weighing fluorescein sodium and water for injection, preparation concentration is 4.0% fluorescein sodium aqueous solution;
(2) take by weighing lissamine green and water for injection, preparation concentration is 4.0% lissamine green aqueous solution;
(3) press volume ratio preparation in 1: the 3 fluorescein sodium aqueous solution of fluorescein sodium and lissamine green and the mixed solution of lissamine green aqueous solution;
(4) be the prepared mixed solution of 0.22u filter filtration step (3) with the aperture, be distributed in the disposable container, can use.
Embodiment 4
Get the prepared dyestuff test paper bar of embodiment 1, infiltrate with physiological buffer, be attached to gently the eye table and carry out eye table cornea, conjunctiva dyeing, after the dyeing patient's symptom is judged, the result shows, judges consistent with the judged result of using fluorescein sodium/lissamine green to dye separately with mixed solution dyeing of the present invention.And, use mixed type of the present invention angle conjunctiva vital stain bar can disposablely finish corneal dyeing and conjunctiva dyeing, not only shortened Diagnostic Time, and also little to ocular tissue's zest, the patient is easy to accept.Find simultaneously to use mixed solution dyeing of the present invention to infiltrate through filter paper and be prepared into the mixed-type ocular surface staining test paper bar, adopt Dispoable medical dialyzing paper bag hermetic package, after ethane via epoxyethane or the co-60 radiation sterilization, be convenient to transportation and storage, clinical easy to use, cross infection has been avoided in disposable use.
Claims (12)
1. a new mixed type cornea vital stain is characterized in that, described stain comprises the mixed solution of fluorescein sodium aqueous solution and lissamine green aqueous solution.
2. stain according to claim 1 is characterized in that, in described fluorescein sodium aqueous solution, the concentration of fluorescein sodium is 0.5%-4.0%w/v.
3. stain according to claim 1 is characterized in that, in described lissamine green aqueous solution, the concentration of lissamine green is 0.5%-4.0%w/v.
4. stain according to claim 1 is characterized in that, in described fluorescein sodium aqueous solution, the concentration of fluorescein sodium is 0.5%-2.0%w/v.
5. stain according to claim 1 is characterized in that, in described lissamine green aqueous solution, the concentration of lissamine green is 0.5%-2.0%w/v.
6. stain according to claim 1 is characterized in that, in described mixed solution, the volume ratio of described fluorescein sodium aqueous solution and described lissamine green aqueous solution is 1: 0.25-1: 3.
7. each described stain according to claim 1-6, wherein said aqueous solution is physiological buffer.
8. stain according to claim 7, wherein said physiological buffer is borate buffer, phosphate buffer or intraocular irrigation flushing liquor.
9. a method for preparing each described stain of claim 1-8 is characterized in that, said method comprising the steps of:
(1) takes by weighing fluorescein sodium and water for injection, preparation fluorescein sodium aqueous solution;
(2) take by weighing lissamine green and water for injection, preparation lissamine green aqueous solution;
(3) in ratio claimed in claim 6, the mixed solution of preparation fluorescein sodium aqueous solution and lissamine green aqueous solution;
(4) with the prepared mixed solution of filter filtration step (3).
10. preparation method according to claim 9 is characterized in that, described method also comprises the described water for injection in step (1) and the step (2) is mixed with phosphate buffer, borate buffer or intraocular irrigation flushing liquor in advance.
11. preparation method according to claim 9 is characterized in that, described method comprises that also infiltrating reagent paper with described mixed solution makes the dyestuff test paper bar.
12. preparation method according to claim 11 is characterized in that, described method also comprises with physiological buffer and infiltrates described dyestuff test paper bar.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100661908A CN103301478A (en) | 2012-03-14 | 2012-03-14 | Novel mixed type cornea and conjuctiva intravital staining agent |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100661908A CN103301478A (en) | 2012-03-14 | 2012-03-14 | Novel mixed type cornea and conjuctiva intravital staining agent |
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| Publication Number | Publication Date |
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| CN103301478A true CN103301478A (en) | 2013-09-18 |
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|---|---|---|---|
| CN2012100661908A Pending CN103301478A (en) | 2012-03-14 | 2012-03-14 | Novel mixed type cornea and conjuctiva intravital staining agent |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT201600127037A1 (en) * | 2016-12-15 | 2018-06-15 | Oftalab S R L | OPHTHALMIC SOLUTION OF LISSAMINA GREEN AND ITS USE IN OPHTHALMOLOGY |
| CN109431451A (en) * | 2018-08-22 | 2019-03-08 | 天津博锐医疗器械有限公司 | A kind of corneal dyeing device |
| CN110836805A (en) * | 2019-11-27 | 2020-02-25 | 天津晶明新技术开发有限公司 | Preparation method of staining agent test strip for active staining |
| CN112336877A (en) * | 2020-11-09 | 2021-02-09 | 天津晶明新技术开发有限公司 | Staining solution for eye surface staining and preparation method thereof |
| CN114642743A (en) * | 2020-12-21 | 2022-06-21 | 天津晶明新技术开发有限公司 | Eye drops of staining agent for staining ocular surface tissue |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201641967U (en) * | 2009-12-30 | 2010-11-24 | 天津晶明新技术开发有限公司 | Mixed-type ocular surface staining test paper |
-
2012
- 2012-03-14 CN CN2012100661908A patent/CN103301478A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201641967U (en) * | 2009-12-30 | 2010-11-24 | 天津晶明新技术开发有限公司 | Mixed-type ocular surface staining test paper |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT201600127037A1 (en) * | 2016-12-15 | 2018-06-15 | Oftalab S R L | OPHTHALMIC SOLUTION OF LISSAMINA GREEN AND ITS USE IN OPHTHALMOLOGY |
| WO2018109704A1 (en) * | 2016-12-15 | 2018-06-21 | Oftalab Srl | Ophthalmic solution of lissamine green and its use in ophthalmology |
| JP2020503290A (en) * | 2016-12-15 | 2020-01-30 | オフタラボ エス アール エル | Lissamine green ophthalmic solution and its use in ophthalmology |
| JP7296316B2 (en) | 2016-12-15 | 2023-06-22 | オフタラボ エス アール エル | Lissamine green ophthalmic solution and its use in ophthalmology |
| CN109431451A (en) * | 2018-08-22 | 2019-03-08 | 天津博锐医疗器械有限公司 | A kind of corneal dyeing device |
| CN110836805A (en) * | 2019-11-27 | 2020-02-25 | 天津晶明新技术开发有限公司 | Preparation method of staining agent test strip for active staining |
| CN112336877A (en) * | 2020-11-09 | 2021-02-09 | 天津晶明新技术开发有限公司 | Staining solution for eye surface staining and preparation method thereof |
| CN114642743A (en) * | 2020-12-21 | 2022-06-21 | 天津晶明新技术开发有限公司 | Eye drops of staining agent for staining ocular surface tissue |
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Application publication date: 20130918 |