CN110819733A - Gene combination for identifying long-grain high-quality indica rice and application thereof - Google Patents

Gene combination for identifying long-grain high-quality indica rice and application thereof Download PDF

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CN110819733A
CN110819733A CN201911156506.0A CN201911156506A CN110819733A CN 110819733 A CN110819733 A CN 110819733A CN 201911156506 A CN201911156506 A CN 201911156506A CN 110819733 A CN110819733 A CN 110819733A
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quality
indica rice
rice
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游艾青
徐华山
周雷
李三和
陈志军
杨国才
刘凯
闸雯俊
李培德
徐得泽
李进波
舒军
李珍莲
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Hubei Academy Of Agricultural Sciences Institute Of Food Crops
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Abstract

The invention provides a gene combination for identifying long-grain high-quality indica rice and application thereof, belonging to the technical field of biological breeding, wherein the gene combination comprises a gs3 gene, a GL7 gene, a SLG7 gene, an ALK gene and a Wx genebA gene. When the combination of the genes provided by the invention can be detected in indica rice, the indica rice is long-grain high-quality indica rice, the selection cost of breeding is greatly reduced, the blindness of parent selection in the breeding process is reduced, and the breeding efficiency is improved.

Description

Gene combination for identifying long-grain high-quality indica rice and application thereof
Technical Field
The invention belongs to the technical field of biological breeding, and particularly relates to a gene combination for identifying long-grain high-quality indica rice and application thereof.
Background
Rice is one of the important food crops in China. With the development of social economy and the improvement of the living standard of people, the demand of consumers on high-quality rice tends to flourish day by day, but the high-end long-grain rice sold in the market is mainly imported Thailand jasmine scented rice, and the domestic long-grain rice is mainly sold in the middle and low-end markets. In order to increase the high-end market share of high-quality long-shaped rice in China and create an internationally known high-end rice brand, the breeding work of new varieties of long-grain high-quality long-shaped rice must be enhanced.
Rice quality includes appearance quality, taste quality, processing quality, etc., wherein appearance quality and taste quality are the focus of consumer attention. Researchers at home and abroad carry out a great deal of research work aiming at the appearance quality and the taste quality, and clone a series of genes influencing the appearance quality and the taste quality of rice. Major genes affecting rice appearance quality include GS3, LGY3, GL7, TGW6, qGL3, GS9, GW5, SLG7 and the like. The main genes influencing the taste quality of rice are Wxa、Wxb、Wxin、Wxmp、WxopALK, AGPL1, SSI, SSIII-2, SSIV-2, SBE3, ISA, AGPS2a, GBSSII, and the like. Because the number and the types of genes influencing the appearance and the taste quality of rice are large, the difference of the functions exerted by different combinations of genes is large, the gene combination for controlling the appearance and the taste quality of long-grain high-quality indica rice is not clear, and the efficiency of screening and breeding long-grain high-quality indica rice varieties by breeders is greatly influenced.
Disclosure of Invention
In view of the above, the present invention aims to provide a gene combination for identifying long-grain high-quality indica rice and applications thereof, and by adopting the gene combination provided by the present invention, the selection cost of breeding is greatly reduced, the blindness of parent selection in the breeding process is reduced, and the breeding efficiency is improved.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides a gene combination for identifying long-grain high-quality indica rice, which comprises a gs3 gene, a GL7 gene, a SLG7 gene, an ALK gene and WxbA gene.
Preferably, the base at position 4211 of exon 8 of the ALK gene is A, the base at position 4342 is G, and the base at position 4343 is C.
Preferably, the base at position 4211 of exon 8 of the ALK gene is G, the base at position 4342 is T, and the base at position 4343 is T.
The invention also provides the application of the gene combination in the technical scheme in the identification of long-grain high-quality indica rice.
Preferably, the application comprises: extracting DNA of indica rice leaf, detecting indica rice gene by using molecular marker, and simultaneously detecting gs3 gene, GL7 gene, SLG7 gene, ALK gene and WxbWhen the gene is expressed, the indica rice is long-grain high-quality indica rice.
Preferably, the base at position 4211 of exon 8 of the ALK gene is A, the base at position 4342 is G, and the base at position 4343 is C.
Preferably, the base at position 4211 of exon 8 of the ALK gene is G, the base at position 4342 is T, and the base at position 4343 is T.
The invention provides a gene combination for identifying long-grain high-quality indica rice and application thereof, wherein the gene combination comprises a gs3 gene, a GL7 gene, a SLG7 gene, an ALK gene and a Wx genebA gene. When the combination of the genes provided by the invention can be detected in indica rice, the indica rice is long-grain high-quality indica rice, the selection cost of breeding is greatly reduced, the blindness of parent selection in the breeding process is reduced, and the selection quality of parents is improvedAnd (4) breeding efficiency.
Detailed Description
The invention provides a gene combination for identifying long-grain high-quality indica rice, which comprises a gs3 gene, a GL7 gene, a SLG7 gene, an ALK gene and WxbA gene. In the present invention, the gs3 gene, GL7 gene and SLG7 gene are major appearance genes of indica rice, and the ALK gene and Wx genebThe gene is the major gene of the taste of indica rice.
In the present invention, the base at position 4211 of exon 8 of ALK gene is preferably A, the base at position 4342 is preferably G, and the base at position 4343 is preferably C, hereinafter referred to as ALK (A-GC).
In the present invention, the base at position 4211 of exon 8 of the ALK gene is preferably G, the base at position 4342 is preferably T, and the base at position 4343 is preferably T, which will be referred to as ALK (G-TT) hereinafter.
The invention also provides the application of the gene combination in the technical scheme in the identification of long-grain high-quality indica rice. In the present invention, the application preferably comprises extracting DNA from leaves of indica rice, detecting genes of indica rice by using molecular markers, and simultaneously detecting gs3 gene, GL7 gene, SLG7 gene, ALK gene and Wx genebWhen the gene is expressed, the indica rice is long-grain high-quality indica rice.
In the present invention, the base at position 4211 of exon 8 of ALK gene is preferably A, the base at position 4342 is preferably G, and the base at position 4343 is preferably C, hereinafter referred to as ALK (A-GC).
In the present invention, the base at position 4211 of exon 8 of the ALK gene is preferably G, the base at position 4342 is preferably T, and the base at position 4343 is preferably T, which will be referred to as ALK (G-TT) hereinafter.
The invention adopts CTAB method to extract plant DNA, and adopts molecular marking method to detect gs3 gene, GL7 gene, SLG7 gene and WxbThe ALK gene is detected by a sequencing method (the molecular markers and primer sequences required by gene detection are shown in Table 1).
TABLE 1 molecular markers and primer sequences for Gene detection
Figure BDA0002284946100000031
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
1. Planting indica rice variety Huzhong No. 5, Yujing 91, Yuzhenxiang, Huangmaoyang, Yangxiao, super Basimadi, 9311, Xiang 5, Huarun No. 2, Jianzhen No. 2, Guichao No. 2 and Teqing.
2. Extracting DNA from leaves in seedling stage, and identifying appearance quality genes (GS3, LGY3, GL7, TGW6, qGL3, GS9, GW5 and SLG7) and taste quality genes (Wx) of the above varieties by molecular marker detection technology and gene sequencing technology respectivelya、Wxb、Wxin、Wxmp、WxopALK, AGPL1, SSI, SSIII-2, SSIV-2, SBE3, ISA, AGPS2a and GBSSII). Among them, the ALK gene 8 exon 4211 base is A, 4342 base is G, 4343 base is C, abbreviated as ALK (A-GC), or ALK gene 8 exon 4211 base is G, 4342 base is T, 4343 base is T, abbreviated as ALK (G-TT), has good taste quality.
3. After the plants are mature, seeds are harvested for analyzing the appearance quality and the taste quality. The appearance quality detector of the rice is used for analyzing the grain length, the grain width and the length-width ratio of different varieties. And (4) grading taste values of rice of different varieties by adopting a rice taste meter.
4. The results of comparing the data on the appearance quality and taste quality of different varieties with the data on the genotype are shown in Table 1, and the common genotypes of long-grain high-quality indica rice, which all contain gs3, GL7, SLG7, ALK and Wx, are foundbA gene.The base at 4211 th site of exon 8 of ALK gene is A, the base at 4342 th site is G, the base at 4343 th site is C, and the ALK is abbreviated as ALK (A-GC), or the base at 4211 th site of exon 8 of ALK gene is G, the base at 4342 th site is T, and the base at 4343 th site is T, and the ALK is abbreviated as ALK (G-TT).
TABLE 2 genotype analysis and appearance and taste quality representation of different varieties
Figure BDA0002284946100000061
Figure BDA0002284946100000071
As can be seen from Table 2, the grains of the cultivars containing gs3, GL7 and SLG7 together were elongated and good in appearance quality, and contained ALK and WxbThe taste quality of the gene variety is better. Contains gs3, GL7, SLG7, ALK and WxbThe genetic variety has good appearance and taste quality.
Example 2
In 2016, parent materials such as Huzhong No. 5, Yujing 91, Yuzhenxiang, Huangmao, Yangxiao, super Basma pedicel, 9311, Xiang 5, Huarun No. 2, Jianzhen No. 2, Gui No. 2, extra green, Hefengzhan, Fudao 99 and the like are planted in the south lake base of Wuhan institute of agricultural science, institute of food crops, Hubei province, and the results of detection on the parent materials by utilizing the gene combination show that only 5 materials such as Huzhong No. 5, Yuzhen, Yujing 91, Yangxiao and Huangmao have long-grain high-quality indica rice gene models, and the selection range of the parent is reduced to 5. Further analyzing the agronomic characters of the plant height, the growth period, the yield and the like of the 5 parent materials, and finally determining and selecting No. 5 and Yuzhenxiang in Hubei as parent materials for breeding long-grain high-quality indica rice due to the short growth period, the weak yield advantage and the poor adhesion and lodging resistance of Yangxi early and Huangmao.
The No. 5 in Huzhong is hybridized with Yuzhenxiang in the Hainan base of agricultural science institute of Hubei province in 2017 in spring, F1 is planted in Wuhan in 2017 in summer, and the young panicles are taken for anther culture to obtain stable flower culture seedlings. 30 bottles of anthers with 3000 anthers are inoculated together to obtain 210 embryoids, the inductivity is 7.0 percent, the seedling rate is 126, and the seedling rate is 60.0 percent. 126 anther culture H0 generation single plants are planted in Hainan in 2018 spring, and 126 anther culture H1 generation strains are planted in Wuhan base of food crop research institute of agricultural academy in Hubei province in summer.
The field agronomic character investigation was carried out on 126 anther culture H1 generation lines, and 6 anther culture lines with excellent agronomic characters were screened according to the plant height, plant type, growth period and indoor seed test results, with the line number and character data (see Table 3). Wherein 18m719 and 18m720 are respectively the No. 5 and Yuzhenxiang of the parent Huzhong, and 18m724, 18m736, 18m750, 18m766, 18m786 and 18m788 are anther culture strains.
Wherein the plant height of 18m766 is shorter than Yuzhenxiang, the growth period is shorter than No. 5 in Ezhong, the yield is high, and the rice quality is good. The method is characterized in that a anther culture strain with the number of 18m766 is determined as a target strain, the anther culture strain is named as Runxiangyu, the anther culture strain is recommended to participate in high-grade high-quality rice district test in Hubei province in 2019, seeds are harvested in summer in 2018 and then sent to a food quality supervision and detection center (Wuhan) in rural areas of agriculture for rice quality analysis, the rice quality reaches the ministerial standard excellent 3 grade, the No. 5 (18m719) rice quality in parent Hubei province reaches the ministerial standard excellent 3 grade, and the Jade needle aroma (18m720) does not reach the ministerial high-quality rice grade due to the fact that rice.
TABLE 3 agronomic performance of the superior anther culture lines
Figure BDA0002284946100000081
Note that SPSS analysis, α ═ 0.05
Rice-quality expressions of tables 418 m719,18m720 and 18m766
Figure BDA0002284946100000082
Figure BDA0002284946100000091
The embodiments can show that the gene combination of the invention can quickly, accurately and efficiently select parent materials and improve the breeding efficiency and accuracy of long-grain high-quality indica rice.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
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Claims (7)

1. A gene combination for identifying long-grain high-quality indica rice is characterized by comprising a gs3 gene, a GL7 gene, a SLG7 gene, an ALK gene and a Wx genebA gene.
2. The combination according to claim 1, wherein the base at position 4211 of exon 8 of ALK gene is A, the base at position 4342 is G, and the base at position 4343 is C.
3. The combination according to claim 1, wherein the base at position 4211 of exon 8 of ALK gene is G, the base at position 4342 is T, and the base at position 4343 is T.
4. Use of the gene combination according to any one of claims 1 to 3 for identifying long-grain high-quality indica rice.
5. The application according to claim 4, wherein the application comprises: extracting DNA of indica rice leaf, detecting indica rice gene by using molecular marker, and simultaneously detecting gs3 gene, GL7 gene, SLG7 gene, ALK gene and WxbWhen the gene is expressed, the indica rice is long-grain high-quality indica rice.
6. The use of claim 5, wherein the base at position 4211 of exon 8 of ALK gene is A, the base at position 4342 is G, and the base at position 4343 is C.
7. The use of claim 5, wherein the base at position 4211 of exon 8 of ALK gene is G, the base at position 4342 is T, and the base at position 4343 is T.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112586343A (en) * 2020-12-04 2021-04-02 国家粳稻工程技术研究中心 Breeding method for improving pollen quantity of japonica rice of restorer line

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112586343A (en) * 2020-12-04 2021-04-02 国家粳稻工程技术研究中心 Breeding method for improving pollen quantity of japonica rice of restorer line

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