CN110791429A - Improved cell culture device capable of improving cell survival rate - Google Patents
Improved cell culture device capable of improving cell survival rate Download PDFInfo
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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Abstract
The invention discloses an improved cell culture device capable of improving the cell survival rate, relates to the technical field of cell culture, and solves the problems that the existing cell culture device mainly carries out culture through a culture dish, the shape of the culture dish is mostly a circular disc-shaped structure, the bottom of the culture dish is provided with a dead angle, the culture dish has an obstruction effect when a culture medium is removed, and the culture medium is inconvenient to directly topple over and place or replace an experimental group. An improved cell culture device capable of improving the survival rate of cells comprises a base; an air inlet pipe row and an air outlet pipe row are arranged on two sides of the top of the base; the rear side of the top of the base is fixedly provided with an air filtering box through bolts; the setting of filter box provides optional cultivation function for culture apparatus, can utilize air inlet fan and housing to filter and inhale gas, makes it accomplish multiunit circulation in culture apparatus inside circulation, guarantees the oxygen suppliment, and sealed circulation through inlet tube row and outlet tube row guarantees gaseous clean.
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to an improved cell culture device capable of improving the cell survival rate.
Background
Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain the main structure and function. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology.
Through search, for example, patent No. CN105505739A discloses a cell culture device, which relates to the field of photobiology and is invented to solve the problem of low light utilization efficiency in cell culture. A cell culture apparatus comprising: a support assembly; the adjusting assembly is arranged on the supporting assembly and comprises a top positioning support and a foot positioning support; the positioning assembly can enable the foot positioning support and the top positioning support to be relatively fixed when the positioning assembly is in a locked state; the culture assembly comprises a culture medium, a top suspension piece and a foot suspension piece, wherein the top suspension piece is connected with the top positioning support, the foot suspension piece is connected with the foot positioning support, and the foot positioning support can drive the relative movement of the top positioning support to drive the foot suspension piece to move relative to the top suspension piece, so that the included angle between the surface of the culture medium and the horizontal plane is changed. The cell culture device is used for microalgae culture.
Further, for example, patent No. CN102719360A discloses a cell culture apparatus comprising: an isolator having a sterile space therein, the sterile space accommodating a cell incubator filled with a culture solution containing cells to be cultured; a sampling unit configured to sample a culture solution in the cell incubator; a transport flow path through which an inside of the sterile space and an outside of the sterile space communicate with each other, the transport flow path being configured to restrict a flow therein in a direction directed from the inside of the sterile space to the outside of the sterile space; and a culture liquid conveying portion configured to convey the sampled culture liquid to the outside of the sterile space via the conveyance flow path.
However, the cell culture device that uses now mainly cultivates through the culture dish, and the shape of culture dish is mostly disc-shaped structure, and the bottom has the dead angle, has the hindrance effect when getting rid of the culture medium, and inconvenient directly topples over the culture medium and places or change the experiment group to generally do not possess the function of controlling the air in the culture room, consequently, unsatisfied current demand, to this we have proposed an improved generation can improve cell culture device of cell survival rate.
Disclosure of Invention
Problem (A)
The invention aims to provide an improved cell culture device capable of improving the cell survival rate, and aims to solve the problems that the currently used cell culture device proposed in the background art mainly carries out culture through a culture dish, the shape of the culture dish is mostly a disc-shaped structure, the bottom of the culture dish has a dead angle, the culture dish has an obstruction effect when a culture medium is removed, the culture medium is inconvenient to directly topple over and place or replace an experimental group, and the function of controlling the air in a culture room is generally unavailable.
(II) technical scheme
In order to achieve the purpose, the invention provides the following technical scheme: an improved cell culture device capable of improving the survival rate of cells comprises a base; five groups of middle frame plates are arranged at the top of the base; an air inlet pipe row and an air outlet pipe row are arranged on two sides of the top of the base; the base top rear side is provided with the gas filtering box through bolt fastening.
Preferably, the base further comprises a side frame; two groups of side frames are integrally arranged on two sides of the top of the base, and five groups of middle frame plates are fixedly arranged in the side frames at equal intervals through bolts; an air inlet pipe row and an air outlet pipe row are respectively arranged on two sides of the side frame; the side frames are provided with five groups of through holes, and the through holes are arranged at the same side and are staggered at equal intervals and the same height; the front side of the middle frame plate is fixedly provided with eight groups of pipe sleeves through bolts.
Preferably, one side of the air inlet pipe row is integrally provided with five groups of side rods, and the end parts of the side rods penetrate through the through holes of the side frames and are fixed on the inner sides of the other group of side frames through bolts; eight groups of screw holes are formed in the front side of each air inlet pipe row side lever, and air pipe joints are fixedly arranged in the screw holes through threaded connection; the bottom of the air inlet pipe row penetrates through each group of side rods to be provided with an air passage; and the bottom of the air inlet pipe row is provided with an air pipe which is connected with the air filtering box in a sealing way.
Preferably, one side of the air outlet pipe row is integrally provided with five groups of side rods, and the end parts of the side rods penetrate through holes of the side frames and are fixed on the inner sides of the other group of side frames through bolts; the structure of the exhaust pipe side bar is consistent with that of the intake pipe side bar; and air passages are arranged at two ends of the air outlet pipe row in a way of penetrating through the side rods of each group.
Preferably, the gas filtering box further comprises a drawing gate and an adjusting knob; the top of the air filtering box is provided with a jack, and a drawing gate is movably arranged in the jack; silica gel layers are adhered to the side and the bottom of the drawing gate; an inverted T-shaped groove is formed in the bottom of the inner side of the air filtering box, and a plurality of groups of heaters are arranged in the inverted T-shaped groove; the heater penetrates through the inner wall of the gas filtering box and is provided with a circuit connected with a power supply, and an adjusting knob is arranged on the outer side of the gas filtering box for control; the notch is opened to air filter box one side and is provided with the air inlet fan through the bolt fastening, and the air inlet fan outside is provided with dustproof filter mantle through the bolt fastening.
Preferably, the pipe sleeve further comprises a jack; edge strips are arranged on two sides of the pipe sleeve, and assembly screw holes are formed in the rear ends of the edge strips; the front sides of the pipe sleeve edge strips are provided with circular grooves, and the circular grooves penetrate through the upper side and the lower side and are respectively provided with four groups of jacks; both ends of the jack are provided with screw holes; two groups of cylinders are arranged at the top of each pipe sleeve, and each cylinder is provided with an air pipe which is communicated with the air inlet pipe row and the air outlet pipe row.
Preferably, the pipe sleeve further comprises an inserting block, a connecting block and a screw rod; four groups of arc-shaped buckle structures are integrally arranged at the bottom of the insertion block, and chamfering is carried out on the sides of the buckles; through holes are formed in the two sides of the inserting block; through holes are formed in the two sides of the connecting block, a screw penetrates through the through hole of the connecting block and the through hole of the inserting block and is inserted into screw holes in the two ends of the inserting hole to be fixed on the two sides of the pipe sleeve, and springs are sleeved at the outer ends of the parts, located on the connecting block and the inserting block, of the screws; cylindrical culture dishes are arranged in the pipe sleeves in a sliding mode.
Preferably, the cylindrical culture dish further comprises pagoda insert columns and sliding supporting plates; the whole cylindrical culture dish is of a cylindrical structure, the two sides and the rear part of the top of the cylindrical culture dish are provided with tangent plane openings, and the outer side edge of the rear end face of the cylindrical culture dish is chamfered; pagoda insert columns are uniformly arranged at the rear ends of the two sides of the front end of the cylindrical culture dish, and the outer surfaces of the pagoda insert columns are matched with the bottom structures of the insert blocks; two groups of sliding supporting plates are integrally arranged at the bottoms of the two sides of the cylindrical culture dish; two groups of sliding grooves are formed in the bottom of each pipe sleeve, the rear sides of the sliding grooves are not communicated in a penetrating manner, and the sliding supporting plates can be connected with the sliding grooves in a sliding manner; the rear ends of the sliding supporting plates are all arranged into inclined planes and are subjected to chamfering treatment; the cylindrical culture dish can be provided with a silica gel pad which is connected with the pipe sleeve in a sealing way.
(III) advantageous effects
The invention provides an improved cell culture device capable of improving the cell survival rate, which is provided with a cylindrical culture dish and a pipe sleeve to provide a storage structure convenient to disassemble and assemble for the culture device, wherein the cylindrical culture dish can be used for placing culture medium to culture cells, when the cylindrical culture dish needs to be replaced or combined, a group of cylindrical culture dishes can be rotated in the circumferential direction, and culture solution is poured out from one side to complete replacement; meanwhile, the arrangement of the silica gel pad can ensure the sealing property of the cylindrical culture dish and ensure filtered gas flowing in the cylindrical culture dish; and pipe sleeve and cylinder type culture dish can be fixed through the auto-lock of pagoda inserted column and inserted block, can make pipe sleeve and cylinder type culture dish produce slight dislocation owing to set up the silica gel pad in the time, the pagoda inserted column can provide partial elasticity reset function with the inserted block for the arc, provides certain centre gripping connection function to culture apparatus.
Secondly, the setting of the air filtering box provides a selectable culture function for the culture device, and the air can be filtered and sucked by the air inlet fan and the housing, so that the air circulates in the culture device to complete multiple groups of circulation, oxygen supply is ensured, and the cleanness of the air is ensured through the sealed circulation of the air inlet pipe row and the air outlet pipe row; meanwhile, a heater is arranged in the air filtering box, the power of the heater can be adjusted to increase or decrease the temperature according to the adjusting knob, a heating function is provided for the culture device, and the culture device can be supplemented when the temperature is insufficient.
Drawings
FIG. 1 is a schematic perspective view of an embodiment of the present invention;
FIG. 2 is a schematic perspective rear view structure in an embodiment of the present invention;
FIG. 3 is a schematic perspective side view of an embodiment of the present invention;
FIG. 4 is a disassembled perspective view of a shroud in an embodiment of the present invention;
FIG. 5 is a disassembled bottom perspective view of the shroud in an embodiment of the present invention;
FIG. 6 is a schematic perspective view of a shroud in an embodiment of the present invention;
FIG. 7 is a schematic perspective view of a cylindrical culture dish according to an embodiment of the present invention;
FIG. 8 is an enlarged partial structural view of part A in the embodiment of the present invention;
in fig. 1 to 8, the correspondence between the part names or lines and the reference numbers is:
1. the device comprises a base, 101, side frames, 2, a middle frame plate, 3, an air inlet pipe row, 4, an air outlet pipe row, 5, an air filter box, 501, a drawing gate, 502, an adjusting knob, 6, a heater, 7, an air inlet fan, 8, a pipe sleeve, 801, an inserting hole, 802, an inserting block, 803, a connecting block, 804, a screw, 9, a cylindrical culture dish, 901, a pagoda inserting column, 902, a sliding supporting plate, 10 and a silica gel pad.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to fig. 1 to 8, an embodiment of the present invention includes: an improved cell culture device capable of improving the survival rate of cells comprises a base 1; wherein, the base 1 also comprises a side frame 101; two groups of side frames 101 are integrally arranged on two sides of the top of the base 1, and five groups of middle frame plates 2 are fixedly arranged in the side frames 101 at equal intervals through bolts; an air inlet pipe row 3 and an air outlet pipe row 4 are respectively arranged at two sides of the side frame 101; the side frames 101 are all provided with five groups of through holes, and the through holes are arranged at the same side at equal intervals and staggered in height; eight groups of pipe sleeves 8 are fixedly arranged on the front side of the middle frame plate 2 through bolts; wherein, the pipe sleeve 8 also comprises an insertion hole 801; edge strips are arranged on two sides of the pipe sleeve 8, and assembly screw holes are formed in the rear ends of the edge strips; the front sides of the edge strips of the pipe sleeves 8 are provided with circular grooves, and the circular grooves penetrate through the upper side and the lower side and are respectively provided with four groups of jacks 801; both ends of the jack 801 are provided with screw holes; the top of the pipe sleeve 8 is provided with two groups of cylinders, and the cylinders are respectively provided with air pipes which are communicated with the air inlet pipe row 3 and the air outlet pipe row 4; five groups of middle frame plates 2 are arranged at the top of the base 1; an air inlet pipe row 3 and an air outlet pipe row 4 are arranged on two sides of the top of the base 1; wherein, one side of the air inlet tube row 3 is integrally provided with five groups of side rods, and the end parts of the side rods penetrate through holes of the side frames 101 and are fixed at the inner sides of the other group of side frames 101 through bolts; eight groups of screw holes are formed in the front side of the side lever of the air inlet pipe row 3, and air pipe joints are fixedly arranged in the screw holes through threaded connection; the bottom of the air inlet pipe row 3 penetrates through each group of side rods to be provided with an air passage; the bottom of the air inlet pipe row 3 is provided with an air pipe which is hermetically connected with the air filtering box 5; five groups of side rods are integrally arranged on one side of the air outlet pipe row 4, and the end parts of the side rods penetrate through holes of the side frames 101 and are fixed on the inner sides of the other group of side frames 101 through bolts; the side lever structure of the air outlet pipe row 4 is consistent with the side lever structure of the air inlet pipe row 3; two ends of the air outlet pipe row 4 penetrate through each group of side rods to be provided with air passages; the rear side of the top of the base 1 is fixedly provided with an air filtering box 5 through bolts; wherein, the air filtering box 5 also comprises a drawing gate 501 and an adjusting knob 502; the top of the air filtering box 5 is provided with a jack, and a drawing gate 501 is movably arranged in the jack; silica gel layers are adhered to the side and the bottom of the drawing gate 501; an inverted T-shaped groove is formed in the bottom of the inner side of the air filtering box 5, and a plurality of groups of heaters 6 are arranged in the inverted T-shaped groove; the heater 6 penetrates through the inner wall of the air filtering box 5 to be connected with a power supply through a circuit, and an adjusting knob 502 is arranged on the outer side of the air filtering box 5 for control; the breach is seted up and is provided with air inlet fan 7 through the bolt fastening to air filter box 5 one side, and the air inlet fan 7 outside is provided with dustproof filter mantle through the bolt fastening.
Wherein, the pipe sleeve 8 also comprises an insert block 802, a connecting block 803 and a screw 804; four groups of arc-shaped buckle structures are integrally arranged at the bottom of the inserting block 802, and chamfering is carried out on the sides of the buckles; through holes are formed in the two sides of the insert block 802; through holes are formed in the two sides of the connecting block 803, a screw 804 penetrates through the through hole of the connecting block 803 and the through hole of the inserting block 802 to be inserted into screw holes in the two ends of the inserting hole 801 to be fixed on the two sides of the pipe sleeve 8, and springs are sleeved on the outer ends of the screw 804 located at the connecting block 803 and the inserting block 802; cylindrical culture dishes 9 are arranged in the pipe sleeves 8 in a sliding manner.
Wherein, the cylindrical culture dish 9 also comprises a pagoda insert column 901 and a sliding supporting plate 902; the whole cylindrical culture dish 9 is of a cylindrical structure, the two sides and the rear part of the top of the cylindrical culture dish are provided with section openings, and the outer side edge of the rear end face of the cylindrical culture dish 9 is chamfered; pagoda insert columns 901 are uniformly arranged at the rear ends of the two sides of the front end of the cylindrical culture dish 9, and the outer surfaces of the pagoda insert columns 901 are matched with the bottom structures of the insert blocks 802; two groups of sliding supporting plates 902 are integrally arranged at the bottoms of the two sides of the cylindrical culture dish 9; the bottom of the pipe sleeve 8 is provided with two groups of sliding grooves, the rear sides of the sliding grooves are not communicated in a penetrating way, and the sliding supporting plate 902 can be connected with the sliding grooves in a sliding way; the rear ends of the sliding supporting plates 902 are all arranged into inclined planes and are chamfered; the cylindrical culture dish 9 can be provided with a silica gel pad 10 which is connected with the pipe sleeve 8 in a sealing way.
The working principle is as follows: during assembly, two groups of side frames 101 are integrally arranged on two sides of the top of the base 1, and five groups of middle frame plates 2 are fixedly arranged in the side frames 101 at equal intervals through bolts; an air inlet pipe row 3 and an air outlet pipe row 4 are respectively arranged at two sides of the side frame 101; five groups of through holes are formed in the side frame 101, and the through holes are arranged at the same side and are staggered at equal intervals and at equal heights; eight groups of pipe sleeves 8 are fixedly arranged on the front side of the middle frame plate 2 through bolts; five groups of side rods are integrally arranged on one side of the air inlet pipe row 3, and the end parts of the side rods penetrate through holes of the side frames 101 and are fixed on the inner sides of the other group of side frames 101 through bolts; eight groups of screw holes are formed in the front side of a side lever of the air inlet pipe row 3, and air pipe joints are fixedly arranged in the screw holes through threaded connection; air passages are arranged at the bottom of the air inlet pipe row 3 in a way of penetrating through the side rods of each group; the bottom of the air inlet pipe row 3 is provided with an air pipe which is hermetically connected with the air filtering box 5; five groups of side rods are integrally arranged on one side of the air outlet pipe row 4, and the end parts of the side rods penetrate through holes of the side frames 101 and are fixed on the inner sides of the other group of side frames 101 through bolts; the side lever structure of the air outlet pipe row 4 is consistent with the side lever structure of the air inlet pipe row 3; air passages are arranged at two ends of the air outlet pipe row 4 through each group of side rods; the top of the air filtering box 5 is provided with a jack, and a drawing gate 501 is movably arranged in the jack; a silica gel layer is adhered to the side and the bottom of the drawing gate 501; an inverted T-shaped groove is formed in the bottom of the inner side of the air filtering box 5, and a plurality of groups of heaters 6 are arranged in the inverted T-shaped groove; a circuit is arranged on the heater 6 penetrating through the inner wall of the air filtering box 5 to be connected with a power supply, and an adjusting knob 502 is arranged on the outer side of the air filtering box 5 to control; a gap is formed in one side of the air filtering box 5, an air inlet fan 7 is fixedly arranged on the side of the air filtering box through a bolt, and a dustproof filter cover is fixedly arranged on the outer side of the air inlet fan 7 through a bolt; edge strips are arranged on two sides of the pipe sleeve 8, and an assembly screw hole is formed in the rear end of each edge strip; a circular groove is formed in the front side of the edge strip of the pipe sleeve 8, and four groups of jacks 801 are formed in the upper side and the lower side of the circular groove respectively; screw holes are formed at two ends of the jack 801; two groups of cylinders are arranged at the top of the pipe sleeve 8, and air pipes are respectively arranged on the cylinders and communicated with the air inlet pipe row 3 and the air outlet pipe row 4; four groups of arc-shaped buckle structures are integrally arranged at the bottom of the insertion block 802, and chamfering is carried out on the sides of the buckles; through holes are formed in the two sides of the insert block 802; through holes are formed in two sides of the connecting block 803, a screw 804 penetrates through the through hole of the connecting block 803 and the through hole of the insert block 802 to be inserted into screw holes at two ends of the insert hole 801 and fixed on two sides of the pipe sleeve 8, and a spring is sleeved on the outer end of the part, located on the connecting block 803 and the insert block 802, of the screw 804; a cylindrical culture dish 9 is arranged in the pipe sleeve 8 in a sliding way; the two sides and the rear part of the top part of the culture dish are provided with section openings, and the outer side edge of the rear end surface of the cylindrical culture dish 9 is chamfered; integrally arranging pagoda insert columns 901 at the rear ends of the two sides of the front end of the cylindrical culture dish 9, and fitting the outer surfaces of the pagoda insert columns 901 with the bottom structures of the insert blocks 802; two groups of sliding supporting plates 902 are integrally arranged at the bottoms of two sides of the cylindrical culture dish 9; two groups of sliding grooves are formed in the bottom of the pipe sleeve 8, the rear sides of the sliding grooves are not communicated in a penetrating mode, and the sliding supporting plate 902 is connected with the sliding grooves in a sliding mode; a silica gel pad 10 is arranged on the cylindrical culture dish 9 and is hermetically connected with the pipe sleeve 8; when the cell culture device is used, the cylindrical culture dish 9 can be directly injected into a culture medium for cell implantation, after a sample is implanted, the cylindrical culture dish 9 is directly inserted into the pipe sleeve 8 in a manner of matching with a sliding structure, and is fixed through an elastic combined structure of the pagoda insert column 901 and the insert block 802; the air sucked in can be filtered by the air inlet fan 7, and the hot air generated by the heater 6 is brought into the culture device for circulation, thereby providing the functions of heating and oxygen supply for cells, and meanwhile, some supplementary volatile matters can be arranged in the air filtering box 5 and flow into each culture dish chamber along with the air through evaporation.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (8)
1. The utility model provides an improved generation can improve cell culture device of cell survival rate which characterized in that: comprises a base (1); five groups of middle frame plates (2) are arranged at the top of the base (1); an air inlet pipe row (3) and an air outlet pipe row (4) are arranged on two sides of the top of the base (1); the rear side of the top of the base (1) is fixedly provided with an air filtering box (5) through a bolt.
2. An improved cell culture apparatus as claimed in claim 1, wherein the apparatus comprises: the base (1) also comprises a side frame (101); two groups of side frames (101) are integrally arranged on two sides of the top of the base (1), and five groups of middle frame plates (2) are fixedly arranged in the side frames (101) at equal intervals through bolts; an air inlet pipe row (3) and an air outlet pipe row (4) are respectively arranged on two sides of the side frame (101); five groups of through holes are formed in each side frame (101), and the through holes are arranged at the same side and are staggered at equal intervals; the front side of the middle frame plate (2) is fixedly provided with eight groups of pipe sleeves (8) through bolts.
3. An improved cell culture apparatus as claimed in claim 2, wherein the apparatus comprises: five groups of side rods are integrally arranged on one side of the air inlet pipe row (3), and the end parts of the side rods penetrate through holes of the side frames (101) and are fixed on the inner sides of the other group of side frames (101) through bolts; eight groups of screw holes are formed in the front side of the side lever of the air inlet pipe row (3), and air pipe joints are fixedly arranged in the screw holes through threaded connection; the bottom of the air inlet pipe row (3) penetrates through each group of side rods to be provided with an air passage; the bottom of the air inlet pipe row (3) is provided with an air pipe which is hermetically connected with the air filtering box (5).
4. An improved cell culture apparatus as claimed in claim 2, wherein the apparatus comprises: five groups of side rods are integrally arranged on one side of the air outlet pipe row (4), and the end parts of the side rods penetrate through holes of the side frames (101) and are fixed on the inner sides of the other group of side frames (101) through bolts; the side lever structure of the air outlet pipe row (4) is consistent with that of the air inlet pipe row (3); and air passages are arranged at two ends of the air outlet pipe row (4) by penetrating through the side rods of each group.
5. An improved cell culture apparatus as claimed in claim 1, wherein the apparatus comprises: the gas filtering box (5) also comprises a drawing gate (501) and an adjusting knob (502); the top of the air filtering box (5) is provided with a jack, and a drawing gate (501) is movably arranged in the jack; silica gel layers are adhered to the side and the bottom of the drawing gate (501); an inverted T-shaped groove is formed in the bottom of the inner side of the air filtering box (5), and a plurality of groups of heaters (6) are arranged in the inverted T-shaped groove; the heater (6) penetrates through the inner wall of the air filtering box (5) to be connected with a power supply through a circuit, and an adjusting knob (502) is arranged on the outer side of the air filtering box (5) for control; the dust-proof filter cover is characterized in that one side of the air filtering box (5) is provided with a notch, an air inlet fan (7) is fixedly arranged on one side of the air filtering box through a bolt, and a dust-proof filter cover is fixedly arranged on the outer side of the air inlet fan (7) through a bolt.
6. An improved cell culture apparatus as claimed in claim 2, wherein the apparatus comprises: the pipe sleeve (8) also comprises an insertion hole (801); edge strips are arranged on two sides of the pipe sleeve (8), and assembly screw holes are formed in the rear ends of the edge strips; circular grooves are formed in the front sides of the edge strips of the pipe sleeves (8), and four groups of jacks (801) are formed in the circular grooves in a penetrating mode and penetrate through the upper side and the lower side respectively; screw holes are formed in two ends of the jack (801); two groups of cylinders are arranged at the top of each pipe sleeve (8), and each cylinder is provided with an air pipe which is communicated with the air inlet pipe row (3) and the air outlet pipe row (4).
7. An improved cell culture apparatus as claimed in claim 6, wherein the apparatus comprises: the pipe sleeve (8) further comprises an insert block (802), a connecting block (803) and a screw rod (804); four groups of arc-shaped buckle structures are integrally arranged at the bottom of the insertion block (802), and chamfering is carried out on the sides of the buckles; through holes are formed in both sides of the inserting block (802); through holes are formed in the two sides of the connecting block (803) and penetrate through the through holes of the connecting block (803) and the through holes of the inserting blocks (802) through screws (804) to be inserted into screw holes at the two ends of the inserting holes (801) to be fixed on the two sides of the pipe sleeve (8), and springs are sleeved on the outer ends of the parts, located on the connecting block (803) and the inserting blocks (802), of the screws (804); cylindrical culture dishes (9) are arranged in the pipe sleeves (8) in a sliding manner.
8. An improved cell culture apparatus as claimed in claim 7, wherein the apparatus comprises: the cylindrical culture dish (9) also comprises a pagoda insert column (901) and a sliding supporting plate (902); the whole cylindrical culture dish (9) is of a cylindrical structure, the two sides and the rear part of the top of the cylindrical culture dish are provided with tangent plane openings, and the outer side edge of the rear end face of the cylindrical culture dish (9) is chamfered; pagoda insert columns (901) are uniformly arranged at the rear ends of the two sides of the front end of the cylindrical culture dish (9), and the outer surfaces of the pagoda insert columns (901) are matched with the bottom structures of the insert blocks (802); two groups of sliding supporting plates (902) are uniformly arranged at the bottoms of the two sides of the cylindrical culture dish (9); two groups of sliding grooves are formed in the bottom of each pipe sleeve (8), the rear sides of the sliding grooves are not communicated in a penetrating mode, and the sliding supporting plates (902) can be connected with the sliding grooves in a sliding mode; the rear ends of the sliding supporting plates (902) are all arranged into inclined planes and are subjected to chamfering treatment; the cylindrical culture dish (9) can be provided with a silica gel pad (10) which is in sealing connection with the pipe sleeve (8).
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| CN201911158477.1A CN110791429B (en) | 2019-11-22 | 2019-11-22 | Improved generation cell culture device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112877209A (en) * | 2021-02-24 | 2021-06-01 | 房文彬 | Stem cell preparation device capable of reducing infection |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5763267A (en) * | 1996-04-16 | 1998-06-09 | Advanced Tissue Sciences | Apparatus for the large scale growth and packaging of cell suspensions and three-dimensional tissue cultures |
| CN203807479U (en) * | 2014-04-09 | 2014-09-03 | 四川农业大学 | Culture tank as well as intermittent submerged culture device and system |
| CN206858585U (en) * | 2017-07-03 | 2018-01-09 | 华北理工大学 | A kind of dual-purpose small-sized fermentation device of laboratory solid, liquid |
| CN110029057A (en) * | 2019-04-19 | 2019-07-19 | 烟台职业学院 | A kind of biotechnology cell culture apparatus |
-
2019
- 2019-11-22 CN CN201911158477.1A patent/CN110791429B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5763267A (en) * | 1996-04-16 | 1998-06-09 | Advanced Tissue Sciences | Apparatus for the large scale growth and packaging of cell suspensions and three-dimensional tissue cultures |
| CN203807479U (en) * | 2014-04-09 | 2014-09-03 | 四川农业大学 | Culture tank as well as intermittent submerged culture device and system |
| CN206858585U (en) * | 2017-07-03 | 2018-01-09 | 华北理工大学 | A kind of dual-purpose small-sized fermentation device of laboratory solid, liquid |
| CN110029057A (en) * | 2019-04-19 | 2019-07-19 | 烟台职业学院 | A kind of biotechnology cell culture apparatus |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112877209A (en) * | 2021-02-24 | 2021-06-01 | 房文彬 | Stem cell preparation device capable of reducing infection |
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