CN110777059A - Plastic suction box type culture dish and using method - Google Patents
Plastic suction box type culture dish and using method Download PDFInfo
- Publication number
- CN110777059A CN110777059A CN201911156600.6A CN201911156600A CN110777059A CN 110777059 A CN110777059 A CN 110777059A CN 201911156600 A CN201911156600 A CN 201911156600A CN 110777059 A CN110777059 A CN 110777059A
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- Prior art keywords
- plastic
- dish
- box
- dish box
- cover material
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- 229920003023 plastic Polymers 0.000 title claims abstract description 53
- 239000004033 plastic Substances 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 11
- 239000000463 material Substances 0.000 claims abstract description 28
- 238000007789 sealing Methods 0.000 claims abstract description 24
- 238000000502 dialysis Methods 0.000 claims abstract description 16
- 239000001963 growth medium Substances 0.000 claims abstract description 15
- 230000001954 sterilising effect Effects 0.000 claims abstract description 9
- 238000004806 packaging method and process Methods 0.000 claims abstract description 8
- 238000005507 spraying Methods 0.000 claims abstract description 6
- 238000001816 cooling Methods 0.000 claims abstract description 4
- 238000010438 heat treatment Methods 0.000 claims abstract description 4
- 238000002844 melting Methods 0.000 claims abstract description 4
- 230000008018 melting Effects 0.000 claims abstract description 4
- 239000007787 solid Substances 0.000 claims abstract description 4
- 230000005484 gravity Effects 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 239000004775 Tyvek Substances 0.000 claims description 2
- 229920000690 Tyvek Polymers 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 abstract description 5
- 238000012856 packing Methods 0.000 abstract description 4
- 239000012528 membrane Substances 0.000 abstract description 2
- 239000002609 medium Substances 0.000 description 8
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- 239000011521 glass Substances 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 3
- 239000002775 capsule Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012943 hotmelt Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
Abstract
The invention relates to a plastic suction box type culture dish and a using method, wherein a culture medium is filled at a position 3-5mm from the bottom in a plastic suction dish box, a solid culture medium is formed after cooling, and a transparent observation cover is buckled in the plastic suction dish box and is in clearance fit; placing the plastic uptake dish box for pouring the culture medium in a mould of a heat sealing machine, covering a dialyzing paper cover material on the plastic uptake dish box, starting the heat sealing machine for pressurization, heating to 98-101 ℃ through a heat sealing press plate on the heat sealing machine, and carrying out hot melting on the dialyzing paper cover material and the large-diameter circular surface of the plastic uptake dish box together to realize the sealing of the dialyzing paper cover material on the plastic uptake dish; sterilizing the packaged blister dish box at high temperature; and (4) carrying out code spraying and marking on the sterilized plastic-absorbing dish box, and then packaging to obtain the product. The dialysis membrane cover material is adopted for sealing, so that bacteria can be isolated from entering the dish body. The plastic suction dish box can avoid the requirement that all dish boxes are used up as soon as possible after the traditional dish box is unpacked, and the packing and storage of other plastic suction dish boxes are not influenced after a single plastic suction dish box is used.
Description
Technical Field
The invention relates to an experimental device for microbial testing, in particular to a plastic suction box type culture dish for microbial culture and a using method thereof.
Background
The microorganism culture dish is a common experimental device in microorganism culture experiments, is generally formed by buckling a glass cover body and a dish body, and is placed in an incubator at 37 ℃ for culture and observation after microorganisms are inoculated to a nutrient medium in the culture dish.
Although automated equipment continues to emerge, culture dishes continue to find widespread use in certain environments, such as: sedimentary bacteria inspection, finger-carried bacteria inspection by operators and the like, but the culture dish has a plurality of defects:
if the packing problem, several glass ware dish is a big packing, uses the storage of other products behind a glass ware dish to receive the influence, has increased the risk that the ware dish that fails in time to use receives the pollution, because most use units only use 1-2 ware dishes usually at every turn, can not once use several ware dish, however in case the combined packaging is opened, the original sterile environment of the ware dish that does not use is also broken, receives the pollution very easily.
Because the traditional single dish is not hermetically packaged, the non-sealed dish is very easily polluted by bacteria on an automatic marking production line, so that code spraying and automatic treatment of subsequent marking procedures are inconvenient to carry out after sterilization, and the minimum packaging unit has no important marking and identification problems, such as batch number, sterilization date, product name, effective period and the like.
Disclosure of Invention
In view of the defects in the prior art, the invention provides a plastic uptake box type culture dish and a using method thereof, and an independent sterile disposable plastic uptake box type culture dish product is realized through the combination of a plastic uptake box with a specific structure, an observation cover and medical dialyzing paper.
In order to achieve the purpose, the invention adopts the technical scheme that: the utility model provides a plastic uptake box-type culture dish, includes dialysis paper lid material, its characterized in that: also comprises a plastic suction vessel box and a transparent observation cover;
the plastic-suction utensil box is in a step shape and comprises a small-diameter circular utensil, a middle-diameter circular wall and a large-diameter circular surface, a circle of inverted groove I is formed between the small-diameter circular utensil and the middle-diameter circular wall, two side walls and a bottom wall of the groove I are embodied in the plastic-suction utensil box to form a circle of convex wall, and two side walls and a bottom wall of the middle-diameter circular wall are embodied in the plastic-suction utensil box to form a circle of groove II;
one end of the transparent observation cover is a circular arc surface, the other end of the transparent observation cover is a concave surface, and the periphery of the concave surface is a circle of convex wall;
transparent observation lid lock dress is in the plastic uptake ware box, and the protruding wall of round of transparent observation lid is placed in round recess II of plastic uptake ware box, protruding wall and II clearance fit of recess, dialysis paper capping material hot melt realizes the sealed of dialysis paper capping material to the plastic uptake ware dish on the major diameter round surface of plastic uptake ware dish.
A using method of a plastic suction box type culture dish is characterized by comprising the following steps:
pouring a culture medium at a position 3-5mm from the bottom in the plastic-absorption dish box through an automatic funnel gravity perfusion machine when the culture medium is in a hot liquid state, cooling to form a solid culture medium, buckling a transparent observation cover in the plastic-absorption dish box, arranging a circle of convex walls of the transparent observation cover in a circle of grooves II of the plastic-absorption dish box, and enabling the convex walls to be in clearance fit with the grooves II;
placing the plastic uptake dish box for pouring the culture medium in a mould of a heat sealing machine, covering a dialyzing paper cover material on the plastic uptake dish box, starting the heat sealing machine for pressurization, heating to 98-101 ℃ through a heat sealing press plate on the heat sealing machine, and carrying out hot melting on the dialyzing paper cover material and the large-diameter circular surface of the plastic uptake dish box together to realize the sealing of the dialyzing paper cover material on the plastic uptake dish;
sterilizing the packaged blister dish box at high temperature of 105 ℃ for 30 minutes;
and (4) carrying out code spraying and marking on the sterilized plastic-absorbing dish box, and then packaging to obtain the product.
The invention has the beneficial effects that: the dialysis membrane cover material is adopted for sealing, so that bacteria can be isolated from entering the dish body.
The invention is suitable for various sterilization types such as high temperature, plasma, ethylene oxide and the like, and can meet the sterilization link in production.
The invention is very convenient in the aspect of subsequent working procedure treatment after packaging and sterilization, such as packaging and code spraying working procedures, and can be finished on an independent sterile plastic-sucking box.
The plastic-absorbing dish box is not a glass product, can be directly packaged by a paper box, and does not influence the sealed storage of unused products after being used alone, thereby improving the microorganism culture quality and the convenience of use.
The plastic suction dish box can avoid the requirement that all dish boxes are used up as soon as possible after the traditional dish box is unpacked, and the packing and storage of other plastic suction dish boxes are not influenced after a single plastic suction dish box is used.
Drawings
FIG. 1 is a perspective view of the present invention;
FIG. 2 is an exploded view of the structure of the present invention;
FIG. 3 is a schematic structural view of the plastic-absorbing capsule of the present invention;
FIG. 4 is a cross-sectional view of the blister capsule of the present invention;
FIG. 5 is a cross-sectional view of a transparent viewing cover of the present invention;
fig. 6 is a state diagram of the present invention in use.
Detailed Description
As shown in fig. 1 to 5, a blister-absorbing box type culture dish comprises a dialysis paper cover material 3, a blister-absorbing dish box 1 and a transparent observation cover 2.
The plastic suction dish box 1 is in a step shape and comprises a small-diameter circular dish 1-1, a middle-diameter circular wall 1-2 and a large-diameter circular surface 1-3, a circle of inverted groove I1-4 is formed between the small-diameter circular dish 1-1 and the middle-diameter circular wall 1-2, two side walls and a bottom wall of the groove I1-4 are embodied in the plastic suction dish box 1 to form a circle of convex wall 1-4-1, and two side walls and a bottom wall of the middle-diameter circular wall 1-2 are embodied in the plastic suction dish box 1 to form a circle of groove II 1-2-1.
One end of the transparent observation cover 2 is a circular arc surface, the other end is a concave surface, and the periphery of the concave surface is a circle of convex wall 2-1.
The transparent observation cover 2 is buckled in the plastic suction dish box 1, a circle of convex walls 2-1 of the transparent observation cover 2 are arranged in a circle of grooves II 1-2-1 of the plastic suction dish box 1, the convex walls 2-1 are in clearance fit with the grooves II 1-2-1, and the dialysis paper cover material 3 is hot-melted on a large-diameter circular surface 1-3 of the plastic suction dish 1-1, so that the sealing of the dialysis paper cover material 3 on the plastic suction dish 1-1 is realized.
The plastic suction dish box 1 is made of PEGT or PE plastic suction film; the transparent observation cover 2 is made of light medical plastic; the dialysis paper cover material 3 is Dupont Tyvek cover material.
As shown in FIG. 6, a method for using a culture dish in a plastic suction box comprises the following steps:
pouring a culture medium 4 at a position 3-5mm from the bottom in the plastic-absorbing dish box 1 through an automatic funnel gravity pouring machine when the culture medium is in a hot state liquid, cooling to form a solid culture medium 1, buckling and installing a transparent observation cover 2 in the plastic-absorbing dish box 1, placing a circle of convex wall 2-1 of the transparent observation cover 2 in a circle of groove II 1-2-1 of the plastic-absorbing dish box 1, and enabling the convex wall 2-1 to be in clearance fit with the groove II 1-2-1;
placing the plastic-absorbing dish box 1 poured with the culture medium 4 in a mold 5 of a heat sealing machine, covering the dialysis paper cover material 3 on the plastic-absorbing dish box 1, starting the heat sealing machine for pressurization, heating to 98-101 ℃ through a heat sealing press plate 6 on the heat sealing machine, and carrying out hot melting on the dialysis paper cover material 3 and the large-diameter circular surface 1-3 of the plastic-absorbing dish box 1 to realize the sealing of the dialysis paper cover material 3 on the plastic-absorbing dish 1-1;
sterilizing the packaged blister dish box 1 at high temperature of 105 ℃ for 30 minutes;
and (3) carrying out code spraying and marking on the sterilized plastic-absorbing dish box 1, and then packaging to obtain the product.
The medium 4 base material is determined as a natural medium (complex medium) or a synthetic medium (synthetic medium) type, such as a serum medium or an agar medium, etc., as required.
Claims (3)
1. The utility model provides a plastic uptake box-type culture dish, includes dialysis paper lid material (3), its characterized in that: also comprises a plastic suction vessel box (1) and a transparent observation cover (2);
the plastic-absorbing dish box (1) is in a step shape and consists of a small-diameter circular dish (1-1), a middle-diameter circular wall (1-2) and a large-diameter circular surface (1-3), a circle of inverted groove I (1-4) is arranged between the small-diameter circular dish (1-1) and the middle-diameter circular wall (1-2),
two side walls and a bottom wall of the groove I (1-4) are embodied in the plastic-sucking dish box (1) to form a circle of convex wall (1-4-1), and two side walls and a bottom wall of the middle-diameter circular wall (1-2) are embodied in the plastic-sucking dish box (1) to form a circle of groove II (1-2-1);
one end of the transparent observation cover (2) is a circular arc surface, the other end of the transparent observation cover is a concave surface, and the periphery of the concave surface is a circle of convex walls (2-1);
the transparent observation cover (2) is buckled in the plastic suction dish box (1), a circle of raised wall (2-1) of the transparent observation cover (2) is arranged in a circle of groove II (1-2-1) of the plastic suction dish box (1), the raised wall (2-1) is in clearance fit with the groove II (1-2-1), and the dialysis paper cover material (3) is hot-melted on a large-diameter circular surface (1-3) of the plastic suction dish (1-1) to realize the sealing of the dialysis paper cover material (3) on the plastic suction dish (1-1).
2. The culture dish with a blister box according to claim 1, wherein: the plastic-absorbing dish box (1) is made of a PEGT or PE plastic-absorbing film; the transparent observation cover (2) is made of light medical plastics; the dialysis paper cover material (3) is a Dupont Tyvek cover material.
3. The use method of the plastic uptake box type culture dish of claim 1, is characterized by comprising the following steps:
pouring a culture medium (4) at a position 3-5mm from the bottom in a plastic-suction dish box (1) through an automatic funnel gravity pouring machine when the culture medium is in a hot liquid state, cooling to form a solid culture medium (1), buckling a transparent observation cover (2) in the plastic-suction dish box (1), arranging a circle of convex walls (2-1) of the transparent observation cover (2) in a circle of grooves II (1-2-1) of the plastic-suction dish box (1), and enabling the convex walls (2-1) to be in clearance fit with the grooves II (1-2-1);
placing a plastic suction dish box (1) poured with a culture medium (4) in a mold (5) of a heat sealing machine, covering a dialyzing paper cover material (3) on the plastic suction dish box (1), starting the heat sealing machine to pressurize, heating to 98-101 ℃ through a heat sealing press plate (6) on the heat sealing machine, and carrying out hot melting on the dialyzing paper cover material (3) and a large-diameter circular surface (1-3) of the plastic suction dish box (1) together to realize the sealing of the dialyzing paper cover material (3) on the plastic suction dish (1-1);
sterilizing the packaged plastic-absorbing dish box (1) at high temperature of 105 ℃ for 30 minutes;
and (3) carrying out code spraying and marking on the sterilized plastic-absorbing dish box (1), and then packaging to obtain the product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911156600.6A CN110777059A (en) | 2019-11-22 | 2019-11-22 | Plastic suction box type culture dish and using method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911156600.6A CN110777059A (en) | 2019-11-22 | 2019-11-22 | Plastic suction box type culture dish and using method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110777059A true CN110777059A (en) | 2020-02-11 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911156600.6A Pending CN110777059A (en) | 2019-11-22 | 2019-11-22 | Plastic suction box type culture dish and using method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110777059A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1876804A (en) * | 2005-05-30 | 2006-12-13 | 株式会社日立制作所 | Cell culturel vessel, production process thereof and cultured cell |
| EP2284252A1 (en) * | 2009-08-13 | 2011-02-16 | Sony DADC Austria AG | Surface-structured device for life-science applications |
| CN205528757U (en) * | 2016-01-28 | 2016-08-31 | 北京农学院 | Special culture dish of spore is produced to rice blast fungus |
| CN207889154U (en) * | 2017-12-02 | 2018-09-21 | 深圳光彩生命工程技术有限公司 | A kind of high-frequency heat sealing machine of intelligent control |
| CN211546522U (en) * | 2019-11-22 | 2020-09-22 | 拓普能量（天津）科技有限公司 | Plastic uptake box type culture dish |
-
2019
- 2019-11-22 CN CN201911156600.6A patent/CN110777059A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1876804A (en) * | 2005-05-30 | 2006-12-13 | 株式会社日立制作所 | Cell culturel vessel, production process thereof and cultured cell |
| EP2284252A1 (en) * | 2009-08-13 | 2011-02-16 | Sony DADC Austria AG | Surface-structured device for life-science applications |
| CN205528757U (en) * | 2016-01-28 | 2016-08-31 | 北京农学院 | Special culture dish of spore is produced to rice blast fungus |
| CN207889154U (en) * | 2017-12-02 | 2018-09-21 | 深圳光彩生命工程技术有限公司 | A kind of high-frequency heat sealing machine of intelligent control |
| CN211546522U (en) * | 2019-11-22 | 2020-09-22 | 拓普能量（天津）科技有限公司 | Plastic uptake box type culture dish |
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Application publication date: 20200211 |