CN110759783A - Compound microorganism organic water-soluble fertilizer and preparation method thereof - Google Patents

Compound microorganism organic water-soluble fertilizer and preparation method thereof Download PDF

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Publication number
CN110759783A
CN110759783A CN201911268862.1A CN201911268862A CN110759783A CN 110759783 A CN110759783 A CN 110759783A CN 201911268862 A CN201911268862 A CN 201911268862A CN 110759783 A CN110759783 A CN 110759783A
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parts
soluble fertilizer
water
compound microbial
organic water
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Inventor
王跃华
付长征
张学珍
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Shandong Jielile Bioengineering Technology Co Ltd
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Shandong Jielile Bioengineering Technology Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C11/00Other nitrogenous fertilisers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor

Abstract

The invention discloses a compound microbial organic water-soluble fertilizer which comprises the following raw materials: the composite microbial organic water-soluble fertilizer has the advantages of simple process, stable property, quick dissolution, strong mixing property, convenient application, high microbial strain purity, strong activity and long survival period, can supplement soil organic carbon sources, condition soil acidification and hardening, improve soil granular structure, enhance water permeability and air permeability and soil fertilizer, thereby accelerating the moving speed of nutrient elements in the soil, regulating the magnetic environment for the growth of crops, improving the nutrient absorption and running capability of the crops, thereby greatly improving the utilization rate of the fertilizer, and having the obvious advantages of safety, pure green, fertilizer saving, pesticide consumption reduction, crop yield increase, crop quality improvement and crop drug residue reduction.

Description

Compound microorganism organic water-soluble fertilizer and preparation method thereof
Technical Field
The invention relates to a fertilizer, in particular to a compound microorganism organic water-soluble fertilizer and a preparation method thereof.
Background
The water-soluble fertilizer is a compound fertilizer containing nitrogen, phosphorus, potassium, calcium, magnesium, trace elements, amino acid, humic acid, alginic acid and the like, which can be completely dissolved in water. The fertilizer is divided into a solid water-soluble fertilizer and a liquid water-soluble fertilizer in form. The fertilizer comprises macroelement water-soluble fertilizer, secondary element water-soluble fertilizer, microelement water-soluble fertilizer, amino acid-containing water-soluble fertilizer, humic acid-containing water-soluble fertilizer, organic water-soluble fertilizer and the like according to the nutrient content.
The water-soluble fertilizers in the current market are various in types, and a large amount of chemical accelerators are added into some products, so that the yield is improved, soil hardening and premature senility of plants are easily caused, the quality of crops is reduced, and the health of human bodies is harmed.
Disclosure of Invention
The invention aims to provide a compound microbial organic water-soluble fertilizer and a preparation method thereof, which aim to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
the compound microbial organic water-soluble fertilizer comprises the following raw materials in parts by weight: 50-80 parts of composite microbial powder, 70-100 parts of active organic matters, 20-40 parts of mineral nutrient components, 10-20 parts of chitin, 8-16 parts of magnetic carriers and 4-8 parts of ethanol.
As a further scheme of the invention: the feed comprises the following raw materials in parts by weight: 60-70 parts of composite microbial powder, 80-90 parts of active organic matters, 25-35 parts of mineral nutrient components, 12-18 parts of chitin, 10-14 parts of magnetic carriers and 5-7 parts of ethanol.
As a further scheme of the invention: the feed comprises the following raw materials in parts by weight: 65 parts of composite microbial powder, 85 parts of active organic matters, 30 parts of mineral nutrient components, 15 parts of chitin, 12 parts of magnetic carriers and 6 parts of ethanol.
As a further scheme of the invention: the compound microorganism strain is formed by proportionally combining bacillus subtilis, trichoderma harzianum and paenibacillus polymyxa.
As a further scheme of the invention: the specific preparation method of the compound microbial powder comprises the following steps:
1) rejuvenation of strains: activating the slant of a single strain at room temperature to rejuvenate the strain;
2) seed preparation: firstly, subpackaging a culture medium; then steam sterilization is carried out; cooling to 30 ℃; then inoculating strains, and respectively inoculating single strains into a culture medium according to the inoculation amount of 3-5%; finally, putting the mixture into a constant-temperature shake flask for fermentation to obtain seed liquid;
3) and (3) fermentation in a tank: putting the seed liquid into a fermentation tank for fermentation, performing solid-liquid separation through a filter press after the fermentation is finished, performing at least two filtering treatments, putting the treated microbial strain filtrate into a storage barrel, and preparing for subsequent drying and dehydration treatment;
4) and (4) introducing the microbial strain filtrate into a spray dryer for spray drying, and drying to obtain the required compound microbial strain powder.
As a further scheme of the invention: the active organic matter is prepared by uniformly mixing one or more than two of water-soluble organic matter humic acid fertilizer, amino acid or alginic acid with trace elements, and adding a chelating agent for chelation.
As a further scheme of the invention: the mineral nutrient component is one or a combination of more of pyrite mineral ash, silicate mineral and nitrate mineral; wherein the mass content of SiO2 is more than or equal to 40%, the mass content of calcium carbonate is more than or equal to 30%, the mass content of iron element is more than or equal to 20%, the mass content of magnesium element is more than or equal to 3%, and the mass content of potassium element is more than or equal to 5%.
As a further scheme of the invention: the magnetic carrier is a mixture consisting of magnet powder, fly ash and humic acid according to a mass ratio of 1-3: 2-3: 1-2, and is ground to be less than 300 meshes.
A preparation method of the compound microbial organic water-soluble fertilizer comprises the following steps:
(1) weighing the raw materials according to the proportion;
(2) adding mineral nutrient components into 4-8 times of water, stirring, heating, dissolving, evaporating water, and controlling content of mineral nutrient components to obtain mineral nutrient solution;
(3) adding the compound microbial powder, chitin and ethanol into the mineral nutrient solution obtained in the step (2), and stirring for 2-6min to obtain a mixed solution;
(4) and (4) adding the active organic matter and the magnetic carrier into the mixed solution obtained in the step (3), and stirring for 12-18min to obtain the required compound microorganism organic water-soluble fertilizer.
Compared with the prior art, the invention has the beneficial effects that:
the fertilizer has the advantages of simple process, stable property, quick dissolution, quick acting, strong mixing property, convenient application, large microbial strain purity, strong activity and long survival period, can supplement organic carbon sources of soil, condition soil acidification and hardening, improve soil granular structure, enhance water permeability and air permeability and soil fertilizer, further accelerate the moving speed of nutrient elements in the soil, adjust the magnetic environment for crop growth, and improve the nutrient absorption and running capacity of crops, thereby greatly improving the utilization rate of the fertilizer.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to specific embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
In the embodiment of the invention, the composite microbial organic water-soluble fertilizer comprises the following raw materials in parts by weight: 50 parts of composite microbial powder, 70 parts of active organic matters, 20 parts of mineral nutrient components, 10 parts of chitin, 8 parts of magnetic carriers and 4 parts of ethanol.
Wherein the compound microorganism strain is prepared by proportionally combining bacillus subtilis, trichoderma harzianum and paenibacillus polymyxa.
The specific preparation method of the compound microbial powder comprises the following steps:
1) rejuvenation of strains: activating the slant of a single strain at room temperature to rejuvenate the strain;
2) seed preparation: firstly, subpackaging a culture medium; then steam sterilization is carried out; cooling to 30 ℃; then inoculating strains, and respectively inoculating single strains into a culture medium according to the inoculation amount of 3%; finally, putting the mixture into a constant-temperature shake flask for fermentation to obtain seed liquid;
3) and (3) fermentation in a tank: putting the seed liquid into a fermentation tank for fermentation, performing solid-liquid separation through a filter press after the fermentation is finished, performing at least two filtering treatments, putting the treated microbial strain filtrate into a storage barrel, and preparing for subsequent drying and dehydration treatment;
4) and (4) introducing the microbial strain filtrate into a spray dryer for spray drying, and drying to obtain the required compound microbial strain powder.
The active organic matter is prepared by uniformly mixing a water-soluble organic matter humic acid fertilizer, amino acid and trace elements in equal ratio and adding a chelating agent for chelation.
The mineral nutrient component is one or a combination of more of pyrite mineral ash, silicate mineral and nitrate mineral; wherein the mass content of SiO2 is more than or equal to 40%, the mass content of calcium carbonate is more than or equal to 30%, the mass content of iron element is more than or equal to 20%, the mass content of magnesium element is more than or equal to 3%, and the mass content of potassium element is more than or equal to 5%.
The magnetic carrier is a mixture consisting of magnet powder, fly ash and humic acid according to the mass ratio of 1:2:1, and is ground into particles with the particle size of less than 300 meshes.
A preparation method of the compound microbial organic water-soluble fertilizer comprises the following steps:
(1) weighing the raw materials according to the proportion;
(2) adding mineral nutrient components into 4 times of water, stirring, heating, dissolving, evaporating water, and controlling content of mineral nutrient components to obtain mineral nutrient solution;
(3) adding the compound microbial powder, chitin and ethanol into the mineral nutrient solution obtained in the step (2), and stirring for 2min to obtain a mixed solution;
(4) and (4) adding an active organic matter and a magnetic carrier into the mixed solution obtained in the step (3), and stirring for 12min to obtain the required compound microorganism organic water-soluble fertilizer.
Example 2
In the embodiment of the invention, the composite microbial organic water-soluble fertilizer comprises the following raw materials in parts by weight: 60 parts of compound microbial powder, 80 parts of active organic matters, 25 parts of mineral nutrient components, 12 parts of chitin, 10 parts of magnetic carriers and 5 parts of ethanol.
Wherein the compound microorganism strain is prepared by proportionally combining bacillus subtilis, trichoderma harzianum and paenibacillus polymyxa.
The specific preparation method of the compound microbial powder comprises the following steps:
1) rejuvenation of strains: activating the slant of a single strain at room temperature to rejuvenate the strain;
2) seed preparation: firstly, subpackaging a culture medium; then steam sterilization is carried out; cooling to 30 ℃; then inoculating strains, and respectively inoculating single strains into a culture medium according to the inoculation amount of 3%; finally, putting the mixture into a constant-temperature shake flask for fermentation to obtain seed liquid;
3) and (3) fermentation in a tank: putting the seed liquid into a fermentation tank for fermentation, performing solid-liquid separation through a filter press after the fermentation is finished, performing at least two filtering treatments, putting the treated microbial strain filtrate into a storage barrel, and preparing for subsequent drying and dehydration treatment;
4) and (4) introducing the microbial strain filtrate into a spray dryer for spray drying, and drying to obtain the required compound microbial strain powder.
The active organic matter is prepared by uniformly mixing amino acid, alginic acid and trace elements in equal ratio and adding a chelating agent for chelation.
The mineral nutrient component is one or a combination of more of pyrite mineral ash, silicate mineral and nitrate mineral; wherein the mass content of SiO2 is more than or equal to 40%, the mass content of calcium carbonate is more than or equal to 30%, the mass content of iron element is more than or equal to 20%, the mass content of magnesium element is more than or equal to 3%, and the mass content of potassium element is more than or equal to 5%.
The magnetic carrier is a mixture consisting of magnet powder, fly ash and humic acid according to the mass ratio of 2:2:1, and is ground into particles with the particle size of less than 300 meshes.
A preparation method of the compound microbial organic water-soluble fertilizer comprises the following steps:
(1) weighing the raw materials according to the proportion;
(2) adding mineral nutrient components into 5 times of water, stirring, heating, dissolving, evaporating water, and controlling content of mineral nutrient components to obtain mineral nutrient solution;
(3) adding the compound microbial powder, chitin and ethanol into the mineral nutrient solution obtained in the step (2), and stirring for 3min to obtain a mixed solution;
(4) and (4) adding an active organic matter and a magnetic carrier into the mixed solution obtained in the step (3), and stirring for 14min to obtain the required compound microorganism organic water-soluble fertilizer.
Example 3
In the embodiment of the invention, the composite microbial organic water-soluble fertilizer comprises the following raw materials in parts by weight: 65 parts of composite microbial powder, 85 parts of active organic matters, 30 parts of mineral nutrient components, 15 parts of chitin, 12 parts of magnetic carriers and 6 parts of ethanol.
Wherein the compound microorganism strain is prepared by proportionally combining bacillus subtilis, trichoderma harzianum and paenibacillus polymyxa.
The specific preparation method of the compound microbial powder comprises the following steps:
1) rejuvenation of strains: activating the slant of a single strain at room temperature to rejuvenate the strain;
2) seed preparation: firstly, subpackaging a culture medium; then steam sterilization is carried out; cooling to 30 ℃; then inoculating strains, and respectively inoculating single strains into a culture medium according to the inoculation amount of 4%; finally, putting the mixture into a constant-temperature shake flask for fermentation to obtain seed liquid;
3) and (3) fermentation in a tank: putting the seed liquid into a fermentation tank for fermentation, performing solid-liquid separation through a filter press after the fermentation is finished, performing at least two filtering treatments, putting the treated microbial strain filtrate into a storage barrel, and preparing for subsequent drying and dehydration treatment;
4) and (4) introducing the microbial strain filtrate into a spray dryer for spray drying, and drying to obtain the required compound microbial strain powder.
The active organic matter is prepared by uniformly mixing water-soluble organic matter humic acid fertilizer, amino acid, alginic acid and trace elements in equal ratio and then adding a chelating agent for chelation.
The mineral nutrient component is one or a combination of more of pyrite mineral ash, silicate mineral and nitrate mineral; wherein the mass content of SiO2 is more than or equal to 40%, the mass content of calcium carbonate is more than or equal to 30%, the mass content of iron element is more than or equal to 20%, the mass content of magnesium element is more than or equal to 3%, and the mass content of potassium element is more than or equal to 5%.
The magnetic carrier is a mixture consisting of magnet powder, fly ash and humic acid according to the mass ratio of 3:2:2, and is ground into particles with the particle size of less than 300 meshes.
A preparation method of the compound microbial organic water-soluble fertilizer comprises the following steps:
(1) weighing the raw materials according to the proportion;
(2) adding mineral nutrient components into 6 times of water, stirring, heating, dissolving, evaporating water, and controlling content of mineral nutrient components to obtain mineral nutrient solution;
(3) adding the compound microbial powder, chitin and ethanol into the mineral nutrient solution obtained in the step (2), and stirring for 4min to obtain a mixed solution;
(4) and (4) adding an active organic matter and a magnetic carrier into the mixed solution obtained in the step (3), and stirring for 15min to obtain the required compound microorganism organic water-soluble fertilizer.
Example 4
In the embodiment of the invention, the composite microbial organic water-soluble fertilizer comprises the following raw materials in parts by weight: 70 parts of composite microbial powder, 90 parts of active organic matters, 35 parts of mineral nutrient components, 18 parts of chitin, 14 parts of magnetic carriers and 7 parts of ethanol.
Wherein the compound microorganism strain is prepared by proportionally combining bacillus subtilis, trichoderma harzianum and paenibacillus polymyxa.
The specific preparation method of the compound microbial powder comprises the following steps:
1) rejuvenation of strains: activating the slant of a single strain at room temperature to rejuvenate the strain;
2) seed preparation: firstly, subpackaging a culture medium; then steam sterilization is carried out; cooling to 30 ℃; then inoculating strains, and respectively inoculating single strains into a culture medium according to the inoculation amount of 4%; finally, putting the mixture into a constant-temperature shake flask for fermentation to obtain seed liquid;
3) and (3) fermentation in a tank: putting the seed liquid into a fermentation tank for fermentation, performing solid-liquid separation through a filter press after the fermentation is finished, performing at least two filtering treatments, putting the treated microbial strain filtrate into a storage barrel, and preparing for subsequent drying and dehydration treatment;
4) and (4) introducing the microbial strain filtrate into a spray dryer for spray drying, and drying to obtain the required compound microbial strain powder.
The active organic matter is prepared by uniformly mixing one or more than two of water-soluble organic matter humic acid fertilizer, amino acid or alginic acid with trace elements, and adding a chelating agent for chelation.
The mineral nutrient component is one or a combination of more of pyrite mineral ash, silicate mineral and nitrate mineral; wherein the mass content of SiO2 is more than or equal to 40%, the mass content of calcium carbonate is more than or equal to 30%, the mass content of iron element is more than or equal to 20%, the mass content of magnesium element is more than or equal to 3%, and the mass content of potassium element is more than or equal to 5%.
The magnetic carrier is a mixture consisting of magnet powder, fly ash and humic acid according to the mass ratio of 1:3:2, and is ground into particles with the particle size of less than 300 meshes.
A preparation method of the compound microbial organic water-soluble fertilizer comprises the following steps:
(1) weighing the raw materials according to the proportion;
(2) adding mineral nutrient components into water 7 times of the weight of the mineral nutrient components, fully stirring, heating, fully dissolving, evaporating water, and controlling the content of the mineral nutrient components to obtain a mineral nutrient solution;
(3) adding the compound microbial powder, chitin and ethanol into the mineral nutrient solution obtained in the step (2), and stirring for 5min to obtain a mixed solution;
(4) and (4) adding an active organic matter and a magnetic carrier into the mixed solution obtained in the step (3), and stirring for 16min to obtain the required compound microorganism organic water-soluble fertilizer.
Example 5
In the embodiment of the invention, the composite microbial organic water-soluble fertilizer comprises the following raw materials in parts by weight: 80 parts of compound microbial powder, 100 parts of active organic matters, 40 parts of mineral nutrient components, 20 parts of chitin, 16 parts of magnetic carriers and 8 parts of ethanol.
Wherein the compound microorganism strain is prepared by proportionally combining bacillus subtilis, trichoderma harzianum and paenibacillus polymyxa.
The specific preparation method of the compound microbial powder comprises the following steps:
1) rejuvenation of strains: activating the slant of a single strain at room temperature to rejuvenate the strain;
2) seed preparation: firstly, subpackaging a culture medium; then steam sterilization is carried out; cooling to 30 ℃; then inoculating strains, and respectively inoculating single strains into a culture medium according to the inoculation amount of 5%; finally, putting the mixture into a constant-temperature shake flask for fermentation to obtain seed liquid;
3) and (3) fermentation in a tank: putting the seed liquid into a fermentation tank for fermentation, performing solid-liquid separation through a filter press after the fermentation is finished, performing at least two filtering treatments, putting the treated microbial strain filtrate into a storage barrel, and preparing for subsequent drying and dehydration treatment;
4) and (4) introducing the microbial strain filtrate into a spray dryer for spray drying, and drying to obtain the required compound microbial strain powder.
The active organic matter is prepared by uniformly mixing water-soluble organic matter humic acid fertilizer, amino acid, alginic acid and trace elements in equal ratio and then adding a chelating agent for chelation.
The mineral nutrient component is one or a combination of more of pyrite mineral ash, silicate mineral and nitrate mineral; wherein the mass content of SiO2 is more than or equal to 40%, the mass content of calcium carbonate is more than or equal to 30%, the mass content of iron element is more than or equal to 20%, the mass content of magnesium element is more than or equal to 3%, and the mass content of potassium element is more than or equal to 5%.
The magnetic carrier is a mixture consisting of magnet powder, fly ash and humic acid according to the mass ratio of 3:3:2, and is ground into particles with the particle size of less than 300 meshes.
A preparation method of the compound microbial organic water-soluble fertilizer comprises the following steps:
(1) weighing the raw materials according to the proportion;
(2) adding mineral nutrient components into 8 times of water, stirring, heating, dissolving, evaporating water, and controlling content of mineral nutrient components to obtain mineral nutrient solution;
(3) adding the compound microbial powder, chitin and ethanol into the mineral nutrient solution obtained in the step (2), and stirring for 6min to obtain a mixed solution;
(4) and (4) adding an active organic matter and a magnetic carrier into the mixed solution obtained in the step (3), and stirring for 18min to obtain the required compound microorganism organic water-soluble fertilizer.
The fertilizer has the advantages of simple process, stable property, quick dissolution, quick acting, strong mixing property, convenient application, large microbial strain purity, strong activity and long survival period, can supplement organic carbon sources of soil, condition soil acidification and hardening, improve soil granular structure, enhance water permeability and air permeability and soil fertilizer, further accelerate the moving speed of nutrient elements in the soil, adjust the magnetic environment for crop growth, and improve the nutrient absorption and running capacity of crops, thereby greatly improving the utilization rate of the fertilizer.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims (9)

1. The compound microbial organic water-soluble fertilizer is characterized by comprising the following raw materials in parts by weight: 50-80 parts of composite microbial powder, 70-100 parts of active organic matters, 20-40 parts of mineral nutrient components, 10-20 parts of chitin, 8-16 parts of magnetic carriers and 4-8 parts of ethanol.
2. The compound microbial organic water-soluble fertilizer according to claim 1, which comprises the following raw materials in parts by weight: 60-70 parts of composite microbial powder, 80-90 parts of active organic matters, 25-35 parts of mineral nutrient components, 12-18 parts of chitin, 10-14 parts of magnetic carriers and 5-7 parts of ethanol.
3. The compound microbial organic water-soluble fertilizer according to claim 2, which comprises the following raw materials in parts by weight: 65 parts of composite microbial powder, 85 parts of active organic matters, 30 parts of mineral nutrient components, 15 parts of chitin, 12 parts of magnetic carriers and 6 parts of ethanol.
4. The compound microbial organic water-soluble fertilizer according to claim 1, wherein the compound microbial strain is prepared by proportionally combining bacillus subtilis, trichoderma harzianum and paenibacillus polymyxa.
5. The compound microbial organic water-soluble fertilizer according to claim 1, wherein the compound microbial powder is prepared by the following specific method:
rejuvenation of strains: activating the slant of a single strain at room temperature to rejuvenate the strain;
seed preparation: firstly, subpackaging a culture medium; then steam sterilization is carried out; cooling to 30 ℃; then inoculating strains, and respectively inoculating single strains into a culture medium according to the inoculation amount of 3-5%; finally, putting the mixture into a constant-temperature shake flask for fermentation to obtain seed liquid;
and (3) fermentation in a tank: putting the seed liquid into a fermentation tank for fermentation, performing solid-liquid separation through a filter press after the fermentation is finished, performing at least two filtering treatments, putting the treated microbial strain filtrate into a storage barrel, and preparing for subsequent drying and dehydration treatment;
and (4) introducing the microbial strain filtrate into a spray dryer for spray drying, and drying to obtain the required compound microbial strain powder.
6. The compound microorganism organic water-soluble fertilizer as claimed in claim 1, wherein the active organic matter is prepared by mixing one or more of water-soluble organic matter humic acid fertilizer, amino acid or alginic acid with trace elements, and chelating with chelating agent.
7. The compound microbial organic water-soluble fertilizer according to claim 1, wherein the mineral nutrient component is one or a combination of several of pyrite ash, silicate minerals and nitrate minerals; wherein the mass content of SiO2 is more than or equal to 40%, the mass content of calcium carbonate is more than or equal to 30%, the mass content of iron element is more than or equal to 20%, the mass content of magnesium element is more than or equal to 3%, and the mass content of potassium element is more than or equal to 5%.
8. The compound microbial organic water-soluble fertilizer according to claim 1, wherein the magnetic carrier is a mixture of magnet powder, fly ash and humic acid in a mass ratio of 1-3: 2-3: 1-2, and is ground to a particle size of 300 meshes or less.
9. A method for preparing the compound microbial organic water-soluble fertilizer as defined in any one of claims 1 to 8, comprising the following steps:
weighing the raw materials according to the proportion;
adding mineral nutrient components into 4-8 times of water, stirring, heating, dissolving, evaporating water, and controlling content of mineral nutrient components to obtain mineral nutrient solution;
adding the compound microbial powder, chitin and ethanol into the mineral nutrient solution obtained in the step (2), and stirring for 2-6min to obtain a mixed solution;
and (4) adding the active organic matter and the magnetic carrier into the mixed solution obtained in the step (3), and stirring for 12-18min to obtain the required compound microorganism organic water-soluble fertilizer.
CN201911268862.1A 2019-12-11 2019-12-11 Compound microorganism organic water-soluble fertilizer and preparation method thereof Pending CN110759783A (en)

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CN113135803A (en) * 2021-06-08 2021-07-20 深圳市绿色农业生物科技有限公司 Disease-preventing insect-preventing environment-friendly microbial water-soluble fertilizer

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CN111492741A (en) * 2020-03-13 2020-08-07 广西壮族自治区农业科学院 Method for improving soil improvement effect of organic fertilizer by using activated amino acid
CN113135803A (en) * 2021-06-08 2021-07-20 深圳市绿色农业生物科技有限公司 Disease-preventing insect-preventing environment-friendly microbial water-soluble fertilizer

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