CN110736852A - -type precise rapid sample introduction system without cross contamination - Google Patents
-type precise rapid sample introduction system without cross contamination Download PDFInfo
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- CN110736852A CN110736852A CN201911211304.1A CN201911211304A CN110736852A CN 110736852 A CN110736852 A CN 110736852A CN 201911211304 A CN201911211304 A CN 201911211304A CN 110736852 A CN110736852 A CN 110736852A
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- valve
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1065—Multiple transfer devices
Abstract
The invention discloses a precise rapid sample introduction system without cross contamination, which comprises a precise injector, a multi-way selector valve, a mixing pool, a peristaltic pump, a three-way valve and a one-way valve, wherein the precise injector is provided with two outlets, the outlet 1 is connected with the one-way valve, the outlet 2 is connected with a main hole of the multi-way selector valve, the multi-way selector valve comprises a valve body, a valve moving plate and a valve fixed plate, the valve moving plate is attached to the valve fixed plate, main holes, a plurality of branch holes and grooves communicated with a specific branch hole 1 are arranged on an attaching surface of the valve fixed plate, a groove 1 for reducing the attaching area, a groove 2 capable of connecting the main hole and the branch hole and a groove 3 corresponding to the branch hole groove of the valve fixed plate are arranged on the attaching surface of the valve moving plate, the two specific branch holes (the branch holes 1 and 2) on the valve fixed plate are communicated when the groove 3 on the valve moving plate is intersected and a passage is formed, the branch hole 1 on the valve fixed plate is connected with the mixing pool and can be communicated with the branch hole 2 on the peristaltic pump, and the branch hole is connected with the peristaltic pump.
Description
Technical Field
The invention relates to an automatic precise liquid sample introduction technology, in particular to precise rapid sample introduction systems without cross contamination.
Background
For an analytical instrument, the accurate quantification is key factors determining the performance of the instrument, and the combination of an injector and a multi-way selector valve is used as a classical quantification mode of various precise instruments, which is , so that the accurate pollution-free quantification is ensured, and meanwhile, the accurate pollution-free quantification is introduced to a conventional analytical instrument for use, the working efficiency is improved, the time required by the working of a sample injection system is shortened, and the practical application has fixed difficulty.
problems exist when the injector and the multi-way selector valve are used for sampling and quantifying in the past, such as how to sample and quantify can prevent cross contamination when the multi-way selector valve rotates, and how to quickly clean the sampling and quantifying device so as to improve the working efficiency of an analysis instrument.
Therefore, on the premise of not changing the sampling and sample introduction accuracy of the precision injector, channels are creatively added on the multi-way selector valve, so that certain branch holes of the multi-way selector valve can be communicated, a peristaltic pump with a high liquid inlet speed can be externally connected to mix sample liquid and clean a mixing pool, and meanwhile, the mode of pre-fetching cleaning liquid, air and redundant reagents is added during liquid taking to prevent the problem of cross contamination caused by rotation of the multi-way selector valve.
Disclosure of Invention
In order to achieve the purpose, the invention provides precise rapid sample introduction systems without cross contamination, which comprise a precise injector, a multi-way selector valve, a mixing pool, a peristaltic pump, a three-way valve and a single-way valve, wherein the precise injector is provided with two outlets, the outlet 1 is connected with the single-way valve, the outlet 2 is connected with a main hole of the multi-way selector valve, the multi-way selector valve comprises a valve body, a valve plate and a valve stator plate, the valve plate is attached to the valve stator plate, main holes, a plurality of branch holes and grooves communicated with specific branch holes 1 are arranged on an attaching surface of the valve stator plate, grooves 1 for reducing the attaching area, grooves 2 capable of connecting the main holes and the branch holes and grooves 3 corresponding to the branch hole grooves of the valve stator plate are arranged on the attaching surface of the valve stator plate, when a passage is formed by intersecting the grooves 3 on the valve stator plate, the specific two branch holes (the branch holes 1 and 2) on the valve stator plate can be communicated, the branch holes 1 on the valve stator plate are connected with the mixing pool, the peristaltic holes can be communicated with the branch holes 1 and the peristaltic pump, and the branch holes are connected with the three-way valve.
, the length of the groove on the valve stator and the length of the groove 3 on the valve rotor should both be less than the distance L + D between the branch hole 1 and the branch hole 2, and the sum of the lengths of the two grooves should not be less than L +2D, where D is the diameter of the branch hole.
In step , the intersection of the groove in the valve plate and the groove 3 in the valve plate forms a passageway that does not intersect the groove 2 in the valve plate.
Step , the groove 1 on the valve plate can be any shape that reduces the contact area between the valve plate and the valve plate, and can be selected according to the need.
, the multi-way selector valve comprises a waste liquid branch hole, an air branch hole and a plurality of reagent branch holes except a main hole, a branch hole 1 and a branch hole 2, wherein the main hole is connected with an outlet 2 of the precision injector, the volume of the connecting pipe is not less than the maximum volume of the cavity of the precision injector, the multi-way selector valve also has a reset function to position the groove 2 on the valve moving piece to a position which is not communicated with any branch hole, the two remaining interfaces after the three-way valve is connected with the peristaltic pump are respectively placed in the cleaning liquid and the waste liquid barrel, and the tail end of the connecting pipe placed in the waste liquid barrel is always higher than the liquid level in the barrel.
, the steps of the sample injection system are complete and include the following:
b1: cleaning a pipeline connected with the precision injector: the multi-way selector valve is rotated to a reset position, the single-way valve is opened, the precision injector pumps the cleaning liquid to a set volume, the single-way valve is closed, the multi-way selector valve is rotated to the position of the waste liquid branch hole, and the injector pushes out the cleaned liquid to the waste liquid branch hole;
b2, cleaning the mixing pool, namely, turning a multi-way selector valve to a position where a branch hole 1 is communicated with a branch hole 2, switching a three-way valve to be in a state of being communicated with cleaning liquid, enabling the cleaning liquid to enter the mixing pool by anticlockwise rotating a peristaltic pump, switching the three-way valve to be in a state of being communicated with a waste liquid port by the peristaltic pump until the amount of the cleaning liquid reaches a set value, continuing to rotate the peristaltic pump anticlockwise for times and then rotating the peristaltic pump clockwise to drain the liquid in the mixing pool;
b3, liquid taking, quantifying and liquid entering, wherein a multi-way selection valve is rotated to a reset position, a single-way valve is opened, a precision injector pumps cleaning liquid to a set volume, the single-way valve is closed, the multi-way selection valve is rotated to an air branch hole position, and the injector pumps air with a fixed volume;
b4: air mixing: the multi-way selector valve is rotated to the position where the branch hole 1 is communicated with the branch hole 2, the three-way valve is switched to be communicated with the waste liquid port, and the peristaltic pump rotates anticlockwise to enable air to enter the mixing pool;
and B5, after the mixing pool is mixed, reacted or detected, the actions of emptying the mixing pool and cleaning the mixing pool in the step B2 are repeated.
Further , the steps may be complete steps from B1 to B5, or any single or multiple adjustment or combination of the order of the steps.
, the selection of the hole position of the multi-way selector valve is precisely positioned by an index plate designed integrally with the valve plate .
Compared with the prior art, the invention has the following advantages:
the quantitative part of sample and washing of this system, the function of waste liquid drainage sets up separately, can reduce the contaminated probability of quantitative part of sample in the aspect of , in addition , utilize the peristaltic pump that the feed liquor rate is faster to wash and drain as power, can improve the work efficiency of this system, moreover, sample quantitative mode and take the mode of prefetching partial washing liquid, air, unnecessary reagent before the ration, can prevent the problem of cross contamination that brings when the multiple-way selector valve rotates.
Drawings
FIG. 1 is a flow diagram of a rapid precision sampling system without cross contamination according to the present invention;
FIG. 2 is a schematic view of the abutting surface and the front surface of the valve stator of FIG. 1;
FIG. 3 is a schematic view of the abutting and mounting surfaces of the valve plate of FIG. 1;
FIG. 4 is a schematic view of the multi-way selector valve of FIGS. 1 and 2, shown in the position where the valve plate is rotated to a position where the branch holes 1 and 2 are not communicated and the multi-way selector valve is not in any communication;
FIG. 5 is a schematic view of the multi-way selector valve shown in FIGS. 1 and 2, shown in the position where the valve plate is rotated to a branch hole 1 and a branch hole 2 that are not communicated, and the multi-way selector valve is rotated to a branch position of ;
fig. 6 is a schematic structural view of the valve plate in fig. 1 and 2, which is turned to a state where the branch hole 1 and the branch hole 2 are communicated, and a schematic channel view thereof.
In the figure, 1-precision injector, 2-multi-way selector valve, 3-mixing pool, 4-peristaltic pump, 5-three-way valve, 6-one-way valve, 7-outlet 1, 8-outlet 2, 9-valve stator, 10-valve rotor, 11-main hole, 12-branch hole 2, 13-branch hole 1, 14-groove 1, 15-groove 2, 16-groove 3, 17-groove 4, 18-waste liquid branch hole, 19-air branch hole and 20-graduated disk.
Detailed Description
The precise rapid sample introduction system of the invention is explained in detail below with reference to the accompanying drawings.
As shown in the figure, the invention provides precise rapid sample introduction systems without cross contamination, which comprise a precise injector (1), a multi-way selector valve (2), a mixing pool (3), a peristaltic pump (4), a three-way valve (5) and a single-way valve (6), wherein the precise injector is provided with two outlets, the outlet 1 (7) is connected with the single-way valve (6), and the outlet 2 (8) is connected with a main hole (11) of a stator plate of the multi-way selector valve.
The multi-way selection valve (2) comprises a valve body, a valve moving plate (10) and a valve fixed plate (9), wherein the valve moving plate (10) is attached to the valve fixed plate (9), main holes (11), a plurality of branch holes and grooves 4 (17) communicated with specific branch holes 1 (12) are formed in the attaching surface of the valve fixed plate, grooves 1 (14) for reducing the attaching area, grooves 2 (15) capable of connecting the main holes and the branch holes and grooves 3 (16) corresponding to the branch hole grooves 4 (17) of the valve fixed plate are formed in the attaching surface of the valve moving plate, the grooves 4 (17) in the valve fixed plate are intersected with the grooves 3 (16) in the valve moving plate and can be communicated with the specific two branch holes (the branch holes 1 and 2) in the valve fixed plate when a passage is formed, the branch holes 1 (12) in the valve fixed plate are connected with a mixing pool (3), the branch holes 2 (13) communicated with the branch holes 1 (12) in the valve fixed plate are connected with a peristaltic pump (4), and a plurality of three-way valves (52) are connected with the peristaltic pump (4).
Claims (8)
- The precise rapid sample introduction system without cross contamination comprises a precise injector, a multi-way selector valve, a mixing pool, a peristaltic pump, a three-way valve and a single-way valve, wherein the precise injector is provided with two outlets, the outlet 1 is connected with the single-way valve, the outlet 2 is connected with a main hole of the multi-way selector valve, the multi-way selector valve comprises a valve body, a valve moving plate and a valve fixed plate, the valve moving plate is attached to the valve fixed plate, main holes, a plurality of branch holes and grooves communicated with specific branch holes 1 are arranged on an attaching surface of the valve fixed plate, grooves 1 capable of reducing attaching areas, grooves 2 capable of connecting the main holes and the branch holes and grooves 3 corresponding to the branch hole grooves of the valve fixed plate are arranged on the attaching surface of the valve moving plate, the branch holes 1 and 2 on the valve fixed plate are communicated with the mixing pool and can be communicated with the branch holes 2 on the valve moving plate and the peristaltic pump when a passage is formed, and the branch holes 1 and the branch holes 2 on the valve fixed plate are communicated with the peristaltic pump and the three-way valve.
- 2. The kinds of precise and rapid sample introduction systems without cross contamination according to claim 1, wherein the length of the groove 3 on the valve plate and the length of the groove 3 on the valve plate are both smaller than the distance L + D between the branch hole 1 and the branch hole 2, and the sum of the lengths of the two grooves is not smaller than L +2D, where D is the diameter of the branch hole.
- 3. The kinds of precise and rapid sampling system without cross contamination as claimed in claim 1, wherein the intersection of the groove 3 on the valve plate and the groove on the valve plate forms a channel without intersection with the groove 2 on the valve plate.
- 4. The kinds of precise and rapid sampling systems without cross contamination according to claim 1, wherein the groove 1 in the valve plate can be any shape that reduces the contact area between the valve plate and the valve plate, and can be selectively arranged or not according to the need.
- 5. The kinds of precise rapid sampling systems without cross contamination according to claim 1, wherein the multi-way selector valve comprises a main hole, a branch hole 1, a branch hole 2, and other branch holes including a waste liquid branch hole, an air branch hole, and a plurality of reagent branch holes, wherein the main hole is connected to the outlet 2 of the precision syringe, the volume of the connecting tube should not be less than the maximum volume of the cavity of the precision syringe, the multi-way selector valve further needs a reset function to position the groove 2 on the valve moving plate to a position not communicated with any branch hole, the three-way valve is connected to the peristaltic pump and then the two remaining ports are placed in the cleaning solution and waste liquid tank, wherein the tail end of the connecting tube in the waste liquid tank is always higher than the liquid level in the tank.
- 6. The kinds of precise and rapid sample introduction systems without cross contamination according to claim 1, wherein the steps of the sample introduction system integration include the following:b1: cleaning a pipeline connected with the precision injector: the multi-way selector valve is rotated to a reset position, the single-way valve is opened, the precision injector pumps the cleaning liquid to a set volume, the single-way valve is closed, the multi-way selector valve is rotated to the position of the waste liquid branch hole, and the injector pushes out the cleaned liquid to the waste liquid branch hole;b2, cleaning the mixing pool, namely, turning a multi-way selector valve to a position where a branch hole 1 is communicated with a branch hole 2, switching a three-way valve to be in a state of being communicated with cleaning liquid, enabling the cleaning liquid to enter the mixing pool by anticlockwise rotating a peristaltic pump, switching the three-way valve to be in a state of being communicated with a waste liquid port by the peristaltic pump until the amount of the cleaning liquid reaches a set value, continuing to rotate the peristaltic pump anticlockwise for times and then rotating the peristaltic pump clockwise to drain the liquid in the mixing pool;b3, liquid taking, quantifying and liquid entering, wherein a multi-way selection valve is rotated to a reset position, a single-way valve is opened, a precision injector pumps cleaning liquid to a set volume, the single-way valve is closed, the multi-way selection valve is rotated to an air branch hole position, and the injector pumps air with a fixed volume;b4: air mixing: the multi-way selector valve is rotated to the position where the branch hole 1 is communicated with the branch hole 2, the three-way valve is switched to be communicated with the waste liquid port, and the peristaltic pump rotates anticlockwise to enable air to enter the mixing pool;and B5, after the mixing pool is mixed, reacted or detected, the actions of emptying the mixing pool and cleaning the mixing pool in the step B2 are repeated.
- 7. The method according to claim 5, wherein the steps are selected from the group consisting of a complete step from B1 to B5, and any single or multiple adjustment or combination of the order of the steps.
- 8. The multi-way selector valve of claim 1, wherein the selection of the aperture position of the multi-way selector valve is precisely located by an indexing disk integrally formed with the valve flap .
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911211304.1A CN110736852A (en) | 2019-12-02 | 2019-12-02 | -type precise rapid sample introduction system without cross contamination |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911211304.1A CN110736852A (en) | 2019-12-02 | 2019-12-02 | -type precise rapid sample introduction system without cross contamination |
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| Publication Number | Publication Date |
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| CN110736852A true CN110736852A (en) | 2020-01-31 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201911211304.1A Pending CN110736852A (en) | 2019-12-02 | 2019-12-02 | -type precise rapid sample introduction system without cross contamination |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113713873A (en) * | 2021-08-30 | 2021-11-30 | 广州市锐博生物科技有限公司 | Sample introduction system and control method thereof |
| CN115015567A (en) * | 2022-08-05 | 2022-09-06 | 南京纳摩尔仪器有限公司 | Ceramic 3D tunnel type analysis module |
| CN115266613A (en) * | 2022-08-10 | 2022-11-01 | 江苏德林环保技术有限公司 | Detection system based on ceramic 3D tunnel integrated chip and use method thereof |
-
2019
- 2019-12-02 CN CN201911211304.1A patent/CN110736852A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113713873A (en) * | 2021-08-30 | 2021-11-30 | 广州市锐博生物科技有限公司 | Sample introduction system and control method thereof |
| CN115015567A (en) * | 2022-08-05 | 2022-09-06 | 南京纳摩尔仪器有限公司 | Ceramic 3D tunnel type analysis module |
| CN115266613A (en) * | 2022-08-10 | 2022-11-01 | 江苏德林环保技术有限公司 | Detection system based on ceramic 3D tunnel integrated chip and use method thereof |
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