CN110721297A

CN110721297A - Compound plant medicine for treating chronic prostatitis and prostatic hyperplasia and preparation method thereof

Info

Publication number: CN110721297A
Application number: CN201911020565.5A
Authority: CN
Inventors: 何静仁; 李玉保
Original assignee: Yunhong Group Co Ltd; Guozhong Xinghe Biomedical Technology Co Ltd
Current assignee: Yunhong Group Co Ltd; Guozhong Xinghe Biomedical Technology Co Ltd
Priority date: 2019-10-25
Filing date: 2019-10-25
Publication date: 2020-01-24

Abstract

The invention discloses a compound botanical drug for treating chronic prostatitis and prostatic hyperplasia and a preparation method thereof, wherein the compound botanical drug comprises the following components: 12-18 parts of yam powder, 8-10 parts of leek seed powder, 8-10 parts of cranberry powder, 12-15 parts of black ginger extract, 5-8 parts of cinnamon extract, 3-5 parts of morinda officinalis, 3-5 parts of poria cocos, 5-8 parts of liquorice, 5-8 parts of lithospermum, 8-10 parts of rheum officinale, 12-15 parts of pumpkin seed powder, 0.1-0.2 part of calcium lactate, 5-8 parts of selenium-enriched protein powder, 2-3 parts of tartaric acid, 3-5 parts of gardenia jasminoides, 0.8-1.2 parts of xylan and 10-12 parts of soluble starch. Through reasonable compatibility of the components, the traditional Chinese medicine composition has the effects of clearing heat and removing toxicity, activating and dissolving stasis, resisting inflammation and easing pain, is suitable for people suffering from prostatitis and prostatic hyperplasia, and can be used for preventing or assisting in treating prostatitis and hyperplasia.

Description

Compound plant medicine for treating chronic prostatitis and prostatic hyperplasia and preparation method thereof

Technical Field

The present invention relates to the field of drug development. More specifically, the invention relates to a composite nutrient for promoting blood circulation to remove blood stasis and resisting prostatitis and hyperplasia and a preparation method thereof.

Background

The prostatic hyperplasia is one of common diseases of middle-aged and old men, and the onset of the prostatic hyperplasia is gradually increased along with the aging of the global population. The incidence of prostatic hyperplasia increases with age, with the common symptoms: frequent micturition, nocturia, urgency, incontinence, dysuria, incomplete urination, residual urine increase, hematuria, urinary infection, cystolith, renal dysfunction, long-term lower urinary tract obstruction, etc.

Although the incidence of prostatitis is high, the cause of prostatitis is still unclear, especially nonbacterial prostatitis, and thus the treatment is mainly for improving symptoms.

At present, prostatitis patients are mostly treated by antibiotics, and long-term use of the antibiotics can cause drug resistance, and has long treatment course, great side effect and low cure rate. Therefore, how to develop a medicine for preventing/treating prostatitis has been receiving more and more attention in recent years.

For example, the Chinese invention patent application number 201210171048.X discloses a medicine or health food composition for preventing or treating prostate diseases, which is a preparation prepared from the following raw materials in parts by weight: 5-9 parts of radish and 1-4 parts of honey. The invention also provides a preparation method and application of the composition. However, the radish juice of the raw product in the invention has the braised spicy taste and poor taste.

Therefore, it is important to provide a health product/medicine with significant effect of relieving prostatitis symptoms, so that patients can get rid of taking antibiotics.

Disclosure of Invention

In order to solve the technical problems, the invention provides a compound botanical drug for treating chronic prostatitis and prostatic hyperplasia and a preparation method thereof, wherein the cranberry powder, the black ginger extract and the cinnamon extract are prepared through a special preparation process, so that the pigment content in the black ginger extract is reduced, the appearance and the taste are improved, the yield and the purity of active ingredients in the black ginger extract and the cinnamon extract are improved, and the content of procyanidine in the cranberry powder is increased. Further, through reasonable compatibility with other components, the Chinese medicinal composition has the effects of clearing heat and removing toxicity, activating and dissolving stasis, resisting inflammation and easing pain, is suitable for the people suffering from prostatitis and prostatic hyperplasia, and can be used for preventing or assisting in treating the prostatitis and the hyperplasia.

To achieve these objects and other advantages in accordance with the present invention, there is provided a compound botanical drug for treating chronic prostatitis and prostatic hyperplasia, comprising, in parts by weight: 12-18 parts of yam powder, 8-10 parts of leek seed powder, 8-10 parts of cranberry powder, 12-15 parts of black ginger extract, 5-8 parts of cinnamon extract, 3-5 parts of morinda officinalis, 3-5 parts of poria cocos, 5-8 parts of liquorice, 5-8 parts of lithospermum, 8-10 parts of rheum officinale, 12-15 parts of pumpkin seed powder, 0.1-0.2 part of calcium lactate, 5-8 parts of selenium-enriched protein powder, 2-3 parts of tartaric acid, 3-5 parts of gardenia jasminoides, 0.8-1.2 parts of xylan and 10-12 parts of soluble starch.

Preferably, the preparation method of the cranberry powder comprises the following steps:

s11, weighing cranberry fruits, soaking for 24-36h, washing for 2-3 times with deionized water, drying, crushing, and sieving to obtain cranberry powder;

s12, adding an extracting solution which is 1-2 times of the cranberry powder in weight into the cranberry powder for mixing reaction, wherein the extracting solution is composed of acetone with a volume fraction of 70%, ethanol with a volume fraction of 85% and a citric acid solution with a mass fraction of 0.1-0.2%, and the volume ratio of the acetone to the ethanol to the citric acid solution is 1:1: 2; stirring at the rotation speed of 150 plus 200rpm and at the temperature of 25 ℃, mixing and reacting for 0.5-1h, and then centrifuging and filtering to obtain a supernatant and a precipitate; adding petroleum ether with volume 1-2 times of the supernatant to carry out degreasing treatment to obtain a first extract;

s13, mixing the precipitate, a buffer solution and deionized water, wherein the weight ratio of the precipitate: buffer solution: deionized water ═ 1 (2-3): (8-10) to obtain a mixed system, recording the total volume value of the mixed system, and adjusting the pH value to 6.8-7; heating the mixed system to 50-60 ℃, and keeping the temperature for 60-75min to obtain an extraction system;

carrying out enzymolysis on the leaching system to obtain an enzymolysis system; wherein, the enzymolysis process comprises the following steps:

carrying out first enzymolysis: adjusting the pH value of the leaching system to 8.5, adding trypsin according to 3% of the weight of the leaching system, fully stirring, heating to 55 ℃ while stirring, and preserving heat for 35min to obtain a first enzymolysis system;

and (3) carrying out second enzymolysis: cooling the first enzymolysis system to 20-25 deg.C, adjusting pH to 3.5, adding pectase 4% of the first enzymolysis system, stirring, heating to 45 deg.C while stirring, and keeping the temperature for 30min to obtain a second enzymolysis system;

and (3) carrying out third enzymolysis: after the second enzymolysis system is cooled to 20-25 ℃, adjusting the pH value to 4.5, adding cellulase according to 3% of the weight of the second enzymolysis system, fully stirring, heating to 55 ℃ while stirring, and preserving heat for 20min to obtain a third enzymolysis system;

s14, loading the column with macroporous adsorption resin, washing the resin with pure water, 5% sodium hydroxide solution and 5% hydrochloric acid solution respectively, and loading a third enzymolysis system which is 3-5 times the column volume and completes enzyme deactivation on the column after the washing treatment is finished; washing the column with 1.5-2 volume fractions of 50% ethanol solution, and washing with pure water to pH 6.8-7.0; eluting with 3-5 column volumes of 60% acetone, and collecting eluate; concentrating the eluent under reduced pressure at 50 deg.C until all acetone is removed; adding water-saturated n-butyl alcohol into the eluent without acetone for extraction for 3 times to remove water-soluble impurities, wherein the volume ratio of the water-saturated n-butyl alcohol: eluent is 2: 1; recovering water saturated n-butanol to obtain a second extract;

s15, mixing the second extract with the first extract, and filtering with a microfiltration ceramic membrane

Controlling the temperature at 55-65 ℃ to obtain microfiltration membrane permeate; filtering the microfiltration membrane permeate through a roll-type ultrafiltration membrane, and controlling the operation temperature to be 55-65 ℃ to obtain ultrafiltration membrane permeate; concentrating the ultrafiltration membrane retentate through a roll-type high-pressure reverse osmosis membrane, and controlling the operation temperature to be 30-40 ℃ to obtain a concentrated solution; and (4) freeze-drying the concentrated solution, and crushing and sieving to obtain the cranberry powder.

Preferably, the preparation method of the black ginger extract comprises the following steps:

s21, soaking the black ginger at 25 ℃ for 10-12h, taking out, washing with deionized water for 2-3 times, drying, grinding, and sieving with a 100-mesh sieve to obtain black ginger powder;

s22, performing supercritical carbon dioxide extraction on the black ginger powder, wherein the extraction pressure is 30Mpa, the extraction temperature is 37 ℃, the CO2 flow rate is 25L/h, and the extraction time is 3-5 hours; introducing 95% ethanol with the weight of 3-5% of the weight of the black ginger powder as a carrying agent, and extracting for 1.5 hours; performing secondary pressure reduction separation at 45 deg.C under 8Mpa and 7Mpa to obtain liposoluble active substances of black rhizoma Zingiberis recens powder;

s23, taking the fat-soluble active substance of the black ginger powder, adding deionized water with the weight 5-10 times of that of the fat-soluble active substance of the black ginger powder to obtain an enzymolysis raw material, and carrying out enzymolysis on the enzymolysis raw material to obtain an enzymolysis system; wherein, the enzymolysis process comprises the following steps:

carrying out first enzymolysis: adjusting the pH value of the enzymolysis raw material to 8.5, adding trypsin according to 2-3% of the weight of the enzymolysis raw material, fully stirring, heating to 42 ℃ while stirring, and preserving heat for 35-40min to obtain a first enzymolysis system;

and (3) carrying out second enzymolysis: after the temperature of the first enzymolysis system is reduced to 20-25 ℃, adjusting the pH value to 4.0, adding pectinase according to 4 percent of the weight of the first enzymolysis system, fully stirring, heating to 45 ℃ while stirring, and preserving heat for 30-35min to obtain a second enzymolysis system;

and (3) carrying out third enzymolysis: after the temperature of the second enzymolysis system is reduced to 20-25 ℃, adjusting the pH value to 5.0, adding cellulase according to 3% of the weight of the second enzymolysis system, fully stirring, heating to 60 ℃ while stirring, and preserving heat for 25-35min to obtain a third enzymolysis system;

s24, after the enzymolysis is finished, heating the obtained third enzymolysis system to 85 ℃, and maintaining for 10min to finish the enzyme deactivation process;

s25, loading the resin into a column by using macroporous adsorption resin, washing the resin by using pure water, a sodium hydroxide solution with the mass fraction of 5% and a hydrochloric acid solution with the volume fraction of 5%, and loading a third enzymolysis system which is 8-10 times of the column volume and finishes enzyme deactivation on the column after the washing treatment is finished; washing the column with 5% by volume of 1.5-2 column volumes of hydrochloric acid, and washing with pure water to pH 6.8-7.0; eluting with 60% acetone, and collecting eluate; concentrating the eluent under reduced pressure at 50 deg.C until all acetone is removed; adjusting the pH value of the eluent without the acetone to 5.5-6.5, adding petroleum ether with the volume 1 time that of the eluent for extraction, removing a lower liquid phase containing pigment, and adding water-saturated n-butyl alcohol into an upper liquid phase for extraction for 3 times to remove water-soluble impurities, wherein the water-saturated n-butyl alcohol is calculated according to the volume ratio: the upper liquid phase is 2.5: 1; recovering water saturated n-butanol to obtain rhizoma Zingiberis recens powder extractive solution;

s26, adding activated carbon 4% of the weight of the black ginger powder extracting solution, stirring uniformly, keeping the temperature at 65 ℃ for 65-85min, centrifuging, filtering the liquid phase after removing the sediment through diatomite to obtain a refined black ginger powder extracting solution, wherein the filtering pressure is controlled at 0.25-0.35 MPa;

s27, concentrating the refined black ginger powder extract until the refined black ginger powder extract is dried to obtain a crude crystal; dissolving the obtained crude crystal with anhydrous ethanol, cooling, recrystallizing at 4-5 deg.C, and vacuum filtering after 12 hr to obtain black rhizoma Zingiberis recens extract crystal; repeating the steps of dissolving with absolute ethyl alcohol and recrystallizing for 2-3 times; and finally drying and crushing the crystals to obtain the black ginger extract.

Preferably, the preparation method of the cinnamon extract comprises the following steps:

(1) weighing cinnamon raw materials, soaking for 24-36h, washing with deionized water for 2-3 times, drying, crushing, and sieving with a 100-mesh sieve to obtain cinnamon powder;

(2) performing supercritical carbon dioxide extraction on the cinnamon powder, wherein the extraction pressure is 25Mpa, the extraction temperature is 35 ℃, the CO2 flow rate is 20L/h, and the extraction time is 3-5 hours; introducing 95% ethanol as carrying agent in an amount of 2-3% of the weight of the cinnamon powder, and extracting for 1 hour; performing secondary pressure reduction separation at 45 deg.C under 7Mpa and 6Mpa to obtain cortex Cinnamomi active substance;

(3) mixing the cinnamon active substance, a buffer solution and deionized water, wherein the raw material powder comprises the following components in percentage by weight: buffer solution: deionized water ═ 1 (3-4): (10-15) to obtain a mixed system, recording the total volume value of the mixed system, and adjusting the pH value to 6.8-7; heating the mixed system to 50-60 ℃, and keeping the temperature for 60-75min to obtain an extraction system;

(3) carrying out enzymolysis on the leaching system to obtain an enzymolysis system; wherein, the enzymolysis process comprises the following steps:

carrying out first enzymolysis: adjusting the pH value of the leaching system to 8.0-9.0, adding trypsin according to 4% of the weight of the leaching system, fully stirring, heating to 50-55 ℃ while stirring, and preserving heat for 30-35min to obtain a first enzymolysis system;

and (3) carrying out second enzymolysis: cooling the first enzymolysis system to 20-25 deg.C, adjusting pH to 3.0-4.0, adding pectase 5% of the first enzymolysis system, stirring, heating to 40-45 deg.C while stirring, and maintaining the temperature for 30-35min to obtain a second enzymolysis system;

and (3) carrying out third enzymolysis: after the temperature of the second enzymolysis system is reduced to 20-25 ℃, adjusting the pH value to 4.5-5.0, adding cellulase according to 2% of the weight of the second enzymolysis system, fully stirring, heating to 50-65 ℃ while stirring, and preserving heat for 20-30min to obtain a third enzymolysis system;

(4) loading the column with macroporous adsorption resin, washing the resin with pure water, 5% by mass of sodium hydroxide solution and 5% by volume of hydrochloric acid solution, and loading a third enzymolysis system which is 5-6 times the column volume and completes enzyme deactivation on the column; washing the column with 1.5-2 column volumes of 5% hydrochloric acid, and washing with pure water to pH 6.8-7.0; eluting with 60% acetone, and collecting eluate; concentrating the eluent under reduced pressure at 50 deg.C until all acetone is removed; adjusting the pH value of the eluent without the acetone to 5.5-6.5, adding petroleum ether with the volume 0.8 times of that of the eluent for extraction, removing a lower liquid phase containing pigment, and adding water-saturated n-butyl alcohol into an upper liquid phase for extraction for 3 times to remove water-soluble impurities, wherein the water-saturated n-butyl alcohol is calculated according to the volume ratio: the upper liquid phase is 2: 1; recovering water saturated n-butanol to obtain cortex Cinnamomi extractive solution;

(5) adding activated carbon into the cinnamon extracting solution according to 3% of the weight of the cinnamon extracting solution, uniformly stirring, preserving the heat at 65 ℃ for 60-90min, centrifuging, and removing sediments to obtain clear liquid;

(6) filtering the clear liquid with microfiltration ceramic membrane at 55-65 deg.C to obtain microfiltration membrane permeate

Liquid; filtering the microfiltration membrane permeate through a roll-type ultrafiltration membrane, and controlling the operation temperature to be 55-65 ℃ to obtain ultrafiltration membrane permeate; concentrating the ultrafiltration membrane retentate through a roll-type high-pressure reverse osmosis membrane, and controlling the operation temperature to be 30-40 ℃ to obtain a concentrated solution; the concentrated solution is the cinnamon extract.

Preferably, the buffer is a phosphate buffer.

Preferably, the first enzymolysis and/or the second enzymolysis and/or the third enzymolysis are carried out while ultrasonic treatment is carried out, wherein the ultrasonic power is 200- & lt 400 & gt W, and the ultrasonic treatment time is 10-15 min.

Also provides a preparation method of the compound botanical medicine for treating chronic prostatitis and prostatic hyperplasia, which comprises the following steps:

s100, preparing cranberry powder, a black ginger extract and a cinnamon extract;

s200, fully mixing morinda officinalis, poria cocos, liquorice, lithospermum, rheum officinale and gardenia jasminoides in parts by weight to obtain a raw material mixture, putting the raw material mixture into a primary fermentation tank, adding deionized water in an amount which is 3-5 times of the weight of the raw material mixture and cellulase in an amount which is 0.3 time of the weight of the raw material mixture, adjusting the pH value to 6.8-7, adjusting the temperature to 30 ℃ for primary fermentation, and separating a liquid first fermentation solution after fermentation for 36 hours; after the first fermentation liquid is separated out, adding deionized water with the weight 0.8 time of that of the residue in the primary fermentation tank and pectinase with the weight 0.2 time of that of the residue in the primary fermentation tank into the residue in the primary fermentation tank, adjusting the pH to 4.0, adjusting the temperature to 45 ℃, performing primary fermentation again, and separating out liquid second fermentation liquid after fermentation for 24 hours; combining the first fermentation liquor and the second fermentation liquor to obtain combined fermentation liquor, centrifuging the combined fermentation liquor, and removing precipitates to obtain fermentation clear liquid;

s300, weighing the yam flour, the leek seed powder, the cranberry powder, the black ginger extract, the cinnamon extract, the pumpkin seed powder, the calcium lactate, the selenium-enriched protein powder, the tartaric acid, the xylan and the soluble starch according to the parts by weight in the claim 1, mixing to obtain a mixture, adding deionized water 2.5 times of the weight of the mixture, the fermentation clear liquid and methanol 2 times of the weight of the fermentation clear liquid and having a volume fraction of 85% of the mixture at the temperature of 10 ℃, stirring at a rotating speed of 150rpm to obtain a stock solution, and then heating to 70 ℃ for 25 min;

s400, filtering the stock solution treated in the step S300 by an ultrafiltration membrane with the molecular weight cutoff of more than or equal to 10000Da to obtain ultrafiltrate;

s500, enabling the ultrafiltrate to sequentially pass through a cation column and an anion column, collecting effluent discharged by the anion column, transferring the effluent into a climbing film evaporator, adjusting the temperature to 37 ℃ and the vacuum degree to-0.05 Mpa, stopping evaporation when the volume of the ultrafiltrate is reduced to 30% of the original volume to obtain a primary concentrated solution, transferring the primary concentrated solution into a scraper type concentrator for continuous concentration, adjusting the temperature to 40 ℃ and the vacuum degree to-0.08 Mpa, and stopping evaporation when the volume of the primary concentrated solution is reduced to 25% of the original volume to obtain a secondary concentrated solution;

s600, transferring the secondary concentrated solution into a crude crystallization tank, standing for 12h at the temperature of 4 ℃, and stirring for 10min at intervals of 2h at a speed of 150 r/min at intervals in the process to obtain a crude crystallization raw material; according to the mass percentage, dehydrating the raw material of the coarse crystal until the water content is less than or equal to 18 percent to obtain the coarse crystal; re-dissolving the crude crystals, adjusting the temperature to 30-35 ℃, adding activated carbon accounting for 1-2% of the weight of the crude crystals, stirring and decoloring, and filtering to remove the activated carbon when the transmittance of a decolored solution is 100%;

s700, transferring the decolorized solution to a crystallizing pan, adjusting the temperature to 30 ℃, adjusting the vacuum degree to-0.068 Mpa, and stopping evaporation when the volume of the decolorized solution is reduced to 15% of the original volume to obtain a decolorized concentrated solution; transferring the decolorized concentrated solution into a crystal growing pot, stirring for 30 minutes at the temperature of 4 ℃ and at the temperature of 150-; and dehydrating the fully crystallized raw materials in the crystal cultivating pot until the water content is less than or equal to 8 percent according to the mass percentage, and drying the raw materials in a vacuum drier for 45min to obtain the compound botanical medicine for treating chronic prostatitis and prostatic hyperplasia.

The invention at least comprises the following beneficial effects:

the method removes the pigment content in the black ginger powder extract through the steps of supercritical carbon dioxide extraction, repeated enzymolysis, macroporous adsorption resin adsorption and the like, increases the content and purity of active substances (such as total polymethoxyflavone and the like), and similarly, prepares the cinnamon extract through the repeated enzymolysis mode, so that the cell wall structure of the cinnamon is damaged, and the nutrient components in cells are fully separated out; meanwhile, the impurities are effectively removed by adopting the processes of low-temperature extraction by supercritical carbon dioxide, decompression separation, repeated enzymolysis, elution by macroporous adsorption resin and the like, and the content of nutrient elements such as general flavone, anthocyanin, vitamin C, protein and the like is greatly improved. Furthermore, the components are reasonably compatible with the components with the anti-inflammatory and analgesic effects such as morinda officinalis, honeysuckle, liquorice, lithospermum, rheum officinale and the like, so that the effects of clearing away heat and toxic materials, promoting blood circulation and stimulating the menstrual flow, resisting bacteria and diminishing inflammation, enhancing immunity and the like can be achieved, and the traditional Chinese medicine composition is suitable for the people with prostatitis and prostatic hyperplasia and can be used for preventing or assisting in treating the prostatitis and the hyperplasia.

Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.

Detailed Description

The present invention is further described in detail below with reference to examples to enable those skilled in the art to practice the invention with reference to the description.

It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.

It is to be noted that the test methods described in the following embodiments are conventional methods unless otherwise specified, and the reagents and materials are commercially available without otherwise specified.

< example 1>

The compound botanical medicine for treating chronic prostatitis and prostatic hyperplasia comprises the following components in parts by weight: 12 parts of yam powder, 8 parts of leek seed powder, 8 parts of cranberry powder, 12 parts of black ginger extract, 5 parts of cinnamon extract, 3 parts of morinda officinalis, 3 parts of poria cocos, 5 parts of liquorice, 5 parts of lithospermum, 8 parts of rheum officinale, 12 parts of pumpkin seed powder, 0.1 part of calcium lactate, 5 parts of selenium-enriched protein powder, 2 parts of tartaric acid, 3 parts of gardenia jasminoides, 0.8 part of xylan and 10 parts of soluble starch.

Proanthocyanidins (PACs) are a general name of a large group of widely existing polyphenol compounds, have been proved to have various biological functions such as bacterial adhesion resistance, oxidation resistance, tumor resistance and the like, and have wide application prospects. Procyanidins are widely found in natural plants such as grape, sea buckthorn, pine bark and cowberry. The cranberry is native to North America, but is successfully cultivated in China at present, and the industrial planting of the cranberry is on the future; in addition, China also has natural resources of other bilberry plants, so that procyanidine extracted from the bilberry plants, especially cranberries, has wide market prospect. Therefore, the invention also provides a preparation method of the cranberry powder, which comprises the following steps:

s12, adding an extracting solution which is 1-2 times of the cranberry powder in weight into the cranberry powder for mixing reaction, wherein the extracting solution is composed of acetone with a volume fraction of 70%, ethanol with a volume fraction of 85% and a citric acid solution with a mass fraction of 0.1-0.2%, and the volume ratio of the acetone to the ethanol to the citric acid solution is 1:1: 2; stirring at the rotation speed of 150 plus 200rpm, mixing and reacting for 0.5-1h, and then centrifuging and filtering to obtain supernatant and precipitate; adding petroleum ether with volume 1-2 times of the supernatant to carry out degreasing treatment to obtain a first extract;

s13, mixing the precipitate, a buffer solution and deionized water, wherein the weight ratio of the precipitate: buffer solution: deionized water ═ 1 (2-3): (8-10) (preferably 1:2.5:9) to obtain a mixed system, recording the total volume value of the mixed system, and adjusting the pH value to 6.8-7; heating the mixed system to 50-60 deg.C, and maintaining the temperature for 60-75min (preferably 70min) to obtain leaching system;

s14, filling columns with D101 macroporous adsorption resin, washing the resin with pure water, a sodium hydroxide solution with the mass fraction of 5% and a hydrochloric acid solution with the volume fraction of 5%, and putting a third enzymatic hydrolysis system which is 3-5 times (preferably 4 times) the column volume and completes enzyme inactivation on the columns after the washing treatment is finished; washing the column with 1.5-2 volume fractions of 50% ethanol solution, and washing with pure water to pH 6.8-7.0; eluting with 3-5 (preferably 4) column volumes of 60% acetone, and collecting eluate; concentrating the eluent under reduced pressure at 50 deg.C until all acetone is removed; adding water-saturated n-butyl alcohol into the eluent without acetone for extraction for 3 times to remove water-soluble impurities, wherein the volume ratio of the water-saturated n-butyl alcohol: eluent is 2: 1; recovering water saturated n-butanol to obtain a second extract;

s15, mixing the second extract with the first extract, and filtering by a microfiltration ceramic membrane, wherein the operation temperature is controlled to be 55-65 ℃ (preferably 60 ℃) to obtain microfiltration membrane permeate; filtering the microfiltration membrane permeate through a roll-type ultrafiltration membrane, and controlling the operation temperature to be 55-65 ℃ (preferably 60 ℃) to obtain ultrafiltration membrane permeate; concentrating the ultrafiltration membrane retentate through a roll-type high-pressure reverse osmosis membrane, and controlling the operation temperature to be 30-40 ℃ (preferably 35 ℃) to obtain a concentrated solution; and (4) freeze-drying the concentrated solution, and crushing and sieving to obtain the cranberry powder.

Meanwhile, the black ginger has the effects of nourishing and strengthening, enhancing energy, reducing blood sugar, recovering physical strength, improving circulation, improving dyspepsia and the like; the main special component is the multi-methoxyl flavonoid, and the effects of resisting oxidation, dilating blood vessels, resisting allergy, resisting obesity and preventing aging are achieved. Therefore, the present invention also provides a method for preparing a black ginger extract, which comprises the following steps:

s21, soaking the black ginger at 25 ℃ for 10-12h (preferably 11h), taking out, washing with deionized water for 2-3 times, drying, grinding, and sieving with a 100-mesh sieve to obtain black ginger powder;

s22, performing supercritical carbon dioxide extraction on the black ginger powder, wherein the extraction pressure is 30Mpa, the extraction temperature is 37 ℃, and CO is adopted₂The flow rate is 25L/h, and the extraction time is 3-5 hours; introducing 95% ethanol with the weight of 3-5% of the weight of the black ginger powder as a carrying agent, and extracting for 1.5 hours; performing secondary pressure reduction separation at 45 deg.C under 8Mpa and 7Mpa to obtain liposoluble active substances of black rhizoma Zingiberis recens powder;

s23, taking the fat-soluble active substance of the black ginger powder, adding deionized water with the weight 5-10 times (preferably 8 times) of that of the fat-soluble active substance to obtain an enzymolysis raw material, and carrying out enzymolysis on the enzymolysis raw material to obtain an enzymolysis system; wherein, the enzymolysis process comprises the following steps:

s25, packing columns (phi 10cm multiplied by 120cm) with HZ816 macroporous adsorption resin, washing the resin with pure water, a sodium hydroxide solution with the mass fraction of 5% and a hydrochloric acid solution with the volume fraction of 5%, and loading a third enzymolysis system which is 8-10 times (preferably 9 times) the column volume and completes enzyme inactivation on the columns after the washing treatment is finished; washing the column with 5% by volume of 1.5-2 column volumes of hydrochloric acid, and washing with pure water to pH 6.8-7.0; eluting with 60% acetone, and collecting eluate; concentrating the eluent under reduced pressure at 50 deg.C until all acetone is removed; adjusting the pH value of the eluent after acetone removal to 5.5-6.5 (preferably 6.0), adding petroleum ether with the volume 1 time that of the eluent for extraction, removing a lower liquid phase containing pigments, and adding water-saturated n-butyl alcohol into the upper liquid phase for extraction for 3 times to remove water-soluble impurities, wherein the water-saturated n-butyl alcohol: the upper liquid phase is 2.5: 1; recovering water saturated n-butanol to obtain rhizoma Zingiberis recens powder extractive solution;

s26, adding activated carbon 4% of the weight of the black ginger powder extract, stirring uniformly, keeping the temperature at 65 ℃ for 65-85min (preferably 75min), centrifuging, removing the sediment, and filtering the liquid phase with diatomite to obtain a refined black ginger powder extract, wherein the filtering pressure is controlled at 0.25-0.35MPa (preferably 0.3 MPa);

Because the polymethoxylated flavonoids in the black ginger are fat-soluble substances, and the black ginger contains the polymethoxylated flavonoids, large-polarity substances such as pectin, protein, flavonoid glycoside and the like, and small-polarity substances such as essential oil, fat-soluble pigments and the like, the difficulty of extraction and later separation and purification of the black ginger extract is undoubtedly increased, and the purple fat-soluble pigments influence the appearance of the product. By the preparation method in the embodiment, the content and purity of the polymethoxylated flavonoids in the black ginger can be greatly improved, and the content of the black purple pigment is obviously reduced, so that the extract has an acceptable appearance.

In addition, the cortex Cinnamomi extract can inhibit the expression of cyclooxygenase-2 and carbon monoxide synthase of RAW2647 cell cultured in vitro, has antiinflammatory effect, has certain inhibition effect on acute and chronic inflammatory reaction, has prevention effect on adjuvant arthritis, and can prevent systemic secondary symptoms. Therefore, the present embodiment also provides a method for preparing a cinnamon extract, comprising:

(1) weighing cinnamon raw materials, soaking for 24-36h, washing for 2-3 times by using deionized water, drying, crushing, and sieving by using a 100-mesh sieve to obtain raw material powder;

(2) performing supercritical carbon dioxide extraction on the cinnamon powder, wherein the extraction pressure is 25Mpa, the extraction temperature is 35 ℃, and CO is added₂The flow rate is 20L/h, and the extraction time is 3-5 hours; introducing 95% ethanol as carrying agent in an amount of 2-3% of the weight of the cinnamon powder, and extracting for 1 hour; performing secondary pressure reduction separation at 45 deg.C under 7Mpa and 6Mpa to obtain cortex Cinnamomi active substance;

(2) mixing the cinnamon active substance, a buffer solution (such as phosphate buffer salt) and deionized water, wherein the raw material powder comprises the following components in percentage by weight: buffer solution: deionized water ═ 1 (3-4): (10-15) (preferably 1:3.5:12) to obtain a mixed system, recording the total volume value of the mixed system, and adjusting the pH value to 6.8-7; heating the mixed system to 50-60 deg.C (preferably 55 deg.C), and maintaining for 60-75min (preferably 65min) to obtain leaching system;

carrying out first enzymolysis: adjusting pH of the leaching system to 8.0-9.0 (preferably 8.5), adding trypsin 4% of the weight of the leaching system, stirring, heating to 50-55 deg.C (preferably 52 deg.C) while stirring, and maintaining the temperature for 30-35min to obtain a first enzymolysis system;

and (3) carrying out second enzymolysis: cooling the first enzymolysis system to 20-25 deg.C, adjusting pH to 3.0-4.0 (preferably 3.5), adding pectase 5% of the first enzymolysis system, stirring, heating to 40-45 deg.C, and keeping the temperature for 30-35min to obtain a second enzymolysis system;

and (3) carrying out third enzymolysis: after the temperature of the second enzymolysis system is reduced to 20-25 ℃, adjusting the pH value to 4.5-5.0 (preferably 4.7), adding cellulase according to 2% of the weight of the second enzymolysis system, fully stirring, heating to 50-65 ℃ (preferably 60 ℃) while stirring, and preserving the heat for 20-30min to obtain a third enzymolysis system;

in addition, in order to further increase the extraction efficiency, the ultrasonic treatment is carried out while the first enzymolysis and/or the second enzymolysis and/or the third enzymolysis is carried out, the ultrasonic power is 200-400W (preferably 300W), and the ultrasonic treatment time is 10-15min (preferably 12min), so that the cell wall/membrane structure is fully destroyed, and the content active ingredients are fully separated out;

(5) adding active carbon in the third enzymolysis system according to 3% of the third enzymolysis system, stirring well, keeping the temperature at 65 deg.C for 60-90min (preferably 75min), centrifuging, and removing the residue to obtain clear solution;

(6) filtering the clear solution with microfiltration ceramic membrane at 55-65 deg.C (preferably 60 deg.C) to obtain microfiltration membrane permeate; filtering the microfiltration membrane permeate through a roll-type ultrafiltration membrane, and controlling the operation temperature to be 55-65 ℃ (preferably 60 ℃) to obtain ultrafiltration membrane permeate; concentrating the ultrafiltration membrane retentate through a roll-type high-pressure reverse osmosis membrane, and controlling the operation temperature to be 30-40 ℃ (preferably 35 ℃) to obtain a concentrated solution; the concentrated solution is radix Puerariae extract/cortex Cinnamomi extract.

< example 2>

Compared with the embodiment 1, the compound botanical drug for treating chronic prostatitis and prostatic hyperplasia in the embodiment is different only in that the compound botanical drug comprises the following components: 18 parts of yam powder, 10 parts of leek seed powder, 10 parts of cranberry powder, 15 parts of black ginger extract, 8 parts of cinnamon extract, 5 parts of morinda officinalis, 5 parts of poria cocos, 8 parts of liquorice, 8 parts of lithospermum, 10 parts of rheum officinale, 15 parts of pumpkin seed powder, 0.2 part of calcium lactate, 8 parts of selenium-enriched protein powder, 3 parts of tartaric acid, 5 parts of gardenia jasminoides, 1.2 parts of xylan and 12 parts of soluble starch.

< example 3>

Compared with the embodiment 1, the compound botanical drug for treating chronic prostatitis and prostatic hyperplasia in the embodiment is different only in that the compound botanical drug comprises the following components: 15 parts of yam powder, 9 parts of leek seed powder, 9 parts of cranberry powder, 13 parts of black ginger extract, 7 parts of cinnamon extract, 4 parts of morinda officinalis, 4 parts of poria cocos, 6 parts of liquorice, 7 parts of lithospermum, 9 parts of rheum officinale, 14 parts of pumpkin seed powder, 0.15 part of calcium lactate, 7 parts of selenium-enriched protein powder, 2.5 parts of tartaric acid, 4 parts of gardenia jasminoides, 1.0 part of xylan and 11 parts of soluble starch.

< cranberry powder test results >

Squeezing fresh cranberries to obtain cranberry pomace; and drying the cranberry pomace at low temperature; adding water into the pomace, wherein the material-liquid ratio of the pomace to the water is 1:5(g/ml), ultrasonically extracting for 15 minutes by utilizing ultrasonic waves with the power of 1.8KW, and filtering to obtain cranberry extracting solution; carrying out ultrafiltration separation on the cranberry extract to obtain a filtrate, wherein the ultrafiltration conditions are as follows: the pressure is 0.5Mpa, the temperature is 35 ℃, and the membrane flux is 250 liters/hour; and concentrating the filtrate, drying in vacuum, and crushing to obtain the cranberry powder serving as the comparative example 1. The content and yield of procyanidin of cranberry powder prepared by the preparation method of the invention in the examples 1-3 are detected, and the results are shown in the table 1.

TABLE 1 cranberry powder procyanidin content and yield

	Procyanidin content (mg/100g)	Procyanidin yield (%)
			Comparative example 3	52.14±5.15	0.07
Example 1	68.79±3.79	0.12
			Example 2	69.43±8.12	0.13
Example 3	70.22±7.15	0.15

As can be seen from table 1, the content and yield of procyanidins in cranberry powder obtained by the preparation method of the present invention are significantly higher than those in comparative example 1, and are respectively increased by 34.6% and 114% at most. Therefore, in the preparation method, the impurities are effectively removed by performing low-temperature extraction on the high-efficiency extracting solution consisting of the organic solvent and combining the processes of repeated enzymolysis, macroporous adsorption resin elution and the like, so that the content and the yield of the procyanidine are greatly improved, and the effects of sterilization, anti-inflammation and the like are fully exerted in the treatment and recovery processes of gynecological inflammation.

< measurement results of Black ginger extract >

Taking black ginger as a raw material, freezing, taking out, crushing and sieving to obtain black ginger powder; weighing black ginger powder according to a material-liquid ratio of 1: adding 100(g/mL) of alkyl pyridine bromide salt aqueous solution with the molar concentration of 1.0mol/L, performing microwave extraction for 10 minutes under the conditions of the microwave power of 300W and the temperature of 45 ℃, naturally cooling to 25 ℃, and collecting microwave extraction liquid; vacuum freeze drying the microwave extract to obtain microwave extract concentrate; performing column chromatography separation on the microwave extraction concentrate, eluting with ethyl acetate, and collecting ethyl acetate eluate; the ethyl acetate was removed and then freeze-dried under vacuum to obtain the anti-aging Heiguan ginger extract as comparative example 2. The results of examination of the extract of Zingiber nigrum L obtained by the preparation methods of examples 1 to 3 of the present invention are shown in Table 2, in order to obtain the yield, purity and appearance evaluation of total polymethoxylated flavones.

TABLE 2 Total polymethoxylated flavones content, purity and appearance of Hei ginger extract

As can be seen from Table 2, the content of total polymethoxylated flavones in the extract of Zingiber officinale Roscoe obtained by the preparation method of the present invention is significantly higher than that of comparative example 2, and the purity is as high as 86%, thus the preparation method of the present invention can effectively remove large polar substances such as pectin, protein, flavonoid glycoside, etc., and small polar substances such as essential oil, liposoluble pigment, etc., and simultaneously significantly reduce the content of black purple pigment, so that the extract has acceptable appearance.

< measurement results of cinnamon extract >

Taking cortex Cinnamomi Japonici fine powder, and adding supercritical CO₂In a fluid extraction kettle; setting supercritical CO₂The fluid extraction pressure is 100-350bar, and the extraction temperature is 35 ℃; extracting cortex Cinnamomi Japonici for 10 min; then setting CO₂The dynamic extraction flow rate is 2.5L/min, and the cassia bark is dynamically extracted for 10 min; collecting the obtained supercritical CO₂Fluid extract of CO₂And (3) completely volatilizing to obtain an extract, adding anhydrous sodium sulfate to dehydrate, filtering and drying to obtain the cinnamon extract serving as a comparative example 3. It was examined with cinnamon extract prepared by the preparation method of the present invention in examples 1 to 3 to obtain cinnamaldehyde, cinnamic acid content, and the results are shown in table 3.

TABLE 3 Cinnamaldehyde, cinnamic acid content of cinnamon extract

	Cinnamic aldehyde content (mg/g)	Cinnamic acid content (mg/g)
			Comparative example 2	16.58±5.35	5.58±1.22
Example 1	25.62±4.42	8.74±1.14
			Example 2	26.71±3.37	8.69±0.85
Example 3	25.74±3.49	8.77±1.21

As can be seen from table 3, the cinnamon extract obtained by the preparation method of the present invention has significantly higher cinnamaldehyde and cinnamic acid content than comparative example 3, and both cinnamaldehyde and cinnamic acid have the functions of inhibiting nitric oxide formation, anti-inflammation, anti-oxidation, dilating vascular smooth muscle, etc., so as to have the effects of activating blood stasis, anti-inflammation, relieving pain, resisting prostatitis and prostatic hyperplasia, etc.

< example 4>

The embodiment also provides a preparation method of the compound botanical medicine for treating chronic prostatitis and prostatic hyperplasia, which comprises the following steps:

s100, preparing cranberry powder, a black ginger extract and a cinnamon extract according to the preparation method of any one of the embodiments 1-3;

s200, fully mixing morinda officinalis, poria cocos, liquorice, lithospermum, rheum officinale and gardenia jasminoides according to the parts by weight in any one of embodiments 1-3 to obtain a raw material mixture, putting the raw material mixture into a primary fermentation tank, adding deionized water in an amount which is 3-5 times the weight of the raw material mixture and cellulase in an amount which is 0.3 time the weight of the raw material mixture, adjusting the pH to 6.8-7, adjusting the pH to 30 ℃ for primary fermentation, and separating a liquid first fermentation solution after fermentation for 36 hours; after the first fermentation liquid is separated out, adding deionized water with the weight 0.8 time of that of the residue in the primary fermentation tank and pectinase with the weight 0.2 time of that of the residue in the primary fermentation tank into the residue in the primary fermentation tank, adjusting the pH to 4.0, adjusting the temperature to 45 ℃, performing primary fermentation again, and separating out liquid second fermentation liquid after fermentation for 24 hours; combining the first fermentation liquor and the second fermentation liquor to obtain combined fermentation liquor, centrifuging the combined fermentation liquor, and removing precipitates to obtain fermentation clear liquid;

s300, weighing the yam flour, the leek seed powder, the cranberry powder, the black ginger extract, the cinnamon extract, the pumpkin seed powder, the calcium lactate, the selenium-enriched protein powder, the tartaric acid, the xylan and the soluble starch according to the parts by weight in any one of the embodiments 1 to 3, mixing to obtain a mixture, adding deionized water 2.5 times of the weight of the mixture, the fermentation clear liquid and methanol 2 times of the weight of the fermentation clear liquid and having a volume fraction of 85% of the mixture to dissolve the mixture at the temperature of 10 ℃, stirring at the rotating speed of 150rpm to obtain a raw liquid, and then heating to 70 ℃ for maintaining for 25 min;

< efficacy evaluation test >

Taking 60 male rats, randomly dividing the rats into a normal control group, a model control group and estradiol group, wherein 10 composite nutrient high, medium and low dose groups are adopted, other 5 groups except the normal control group are surgically removed from both sides of testes, feeding the rats for 7d after the operation, injecting 4mg/kg of testosterone propionate subcutaneously every day for 30d continuously to induce prostatic hyperplasia, and the normal control group and the model control group are drenched with 20ml/kg of distilled water. Estradiol injection under skin of estradiol group is 2.5mg/kg, high, medium and low dosage groups are drenched with 5g, 2.5g and 1.5g of the compound botanical of the invention (hereinafter all the compound botanical) once a day for 30 days continuously, the compound botanical is killed after stopping the drug administration, blood is taken to measure blood testosterone, prostate compound leaves are taken to weigh, and gland coefficient is calculated; the glandular epithelial cell height and acinar cavity diameter were measured, as well as the glandular tissue dihydrotestosterone content, and the results are shown in tables 4-5.

TABLE 4 Effect of Compound botanical on glandular coefficient, glandular cell height and glandular lumen diameter

5 Effect of compound botanical drug on serum testosterone and glandular tissue dihydrotestosterone

Group of	Serum testosterone (ng/mL)	Glandular tissue dihydrotestosterone (pg/g)
			Model control group	14.5±3.7	16.2±2.9
Normal control group	6.8±1.1	7.5±1.4
			Estradiol group	11.6±2.3	13.3±2.9
High dose group	11.2±2.4	13.3±3.1
			Middle dose group	14.1±2.8	14.2±2.8
Low dose group	13.8±2.1	15.4±2.7

As can be seen from the results in tables 4 to 5, compared with the model control group, the hyperplasia of the prostate tissues of the rats in the normal control group, the estradiol group and the high-dose group is not obvious, and the difference between the groups has significant significance, so that the compound nutrient has an inhibition effect on the hyperplasia of the prostate tissues induced by testosterone propionate, and is beneficial to treating prostatitis and hyperplasia.

Selecting a subject: 40 patients diagnosed with prostatic hyperplasia and prostatitis were selected and randomly divided into experimental groups and control groups. All patients were diagnosed with benign prostatic hyperplasia by digital rectal examination and B-ultrasound examination. The diagnosis of prostatitis is conformed; digital rectal exam can palpate the full prostate with mild tooth tenderness. Abnormally increased inflammatory cells were visible in the prostate fluid under reduced pressure, but no bacterial growth. Control group: the levofloxacin capsule is orally taken, 0.4 g/time and 1 time/d; treatment groups: the compound nutrient is given on the basis of a control group, 0.5g/kg, 1 time/d; both groups of patients were treated continuously for four weeks.

After four weeks, the relevant index detection was performed, and the results are shown in tables 6 to 7. Selecting indexes: (1) detrusor contraction function: residual urine volume of bladder, maximum urine flow rate, bladder detrusor energy storage; (2) inflammatory factors: serum IL-1. beta., IL-2 and IL-10 were measured using a double antibody sandwich ELISA.

TABLE 6 Effect of Complex Nutrients on detrusor contraction function

As can be seen from Table 6, the residual urine volume in the urinary bladder after treatment was decreased in both groups of patients compared to before treatment, but the residual urine volume in the treated group was significantly less than that in the control group; the maximum uroflow rate and bladder detrusor energy storage of the two groups were increased, and the treatment effect of the treatment group was better than that of the control group. As can be seen from the data in the table, the compound nutrient of the invention has certain relieving effect on the hyperplasia of prostate.

TABLE 7 Effect of Complex Nutrients on inflammatory factors

Compared with the IL-1 beta, IL-2 and IL-10 levels of two groups of patients, the three levels are obviously reduced, which shows that the level of inflammatory factors in serum is reduced, but the IL-1 beta, IL-2 and IL-10 in a treatment group are reduced by 42 percent, 31 percent and 39 percent respectively before and after treatment, which shows that the anti-inflammatory effect of the compound nutrient is obviously better than that of a control group.

It should be noted that the technical solutions in the above embodiments 1 to 4 can be arbitrarily combined, and the technical solutions obtained after the combination all belong to the protection scope of the present invention.

In conclusion, the pigment content in the black ginger powder extract is removed through the steps of supercritical carbon dioxide extraction, repeated enzymolysis, macroporous adsorption resin adsorption and the like, the content and the purity of active substances (such as total polymethoxyflavone and the like) of the black ginger powder extract are increased, and similarly, the cinnamon extract is prepared through the repeated enzymolysis mode, so that the cell wall structure of cinnamon is damaged, and the nutrient components in cells are fully separated out; meanwhile, the impurities are effectively removed by adopting the processes of low-temperature extraction by supercritical carbon dioxide, decompression separation, repeated enzymolysis, elution by macroporous adsorption resin and the like, and the content of nutrient elements such as general flavone, anthocyanin, vitamin C, protein and the like is greatly improved. Furthermore, the components are reasonably compatible with the components with the anti-inflammatory and analgesic effects such as morinda officinalis, honeysuckle, liquorice, lithospermum, rheum officinale and the like, so that the effects of clearing away heat and toxic materials, promoting blood circulation and stimulating the menstrual flow, resisting bacteria and diminishing inflammation, enhancing immunity and the like can be achieved, and the traditional Chinese medicine composition is suitable for the people with prostatitis and prostatic hyperplasia and can be used for preventing or assisting in treating the prostatitis and the hyperplasia.

The number of apparatuses and the scale of the process described herein are intended to simplify the description of the present invention. Applications, modifications and variations of the present invention will be apparent to those skilled in the art.

While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable to various fields of endeavor for which the invention may be embodied with additional modifications as would be readily apparent to those skilled in the art, and the invention is therefore not limited to the details given herein and to the examples shown and described without departing from the generic concept as defined by the claims and their equivalents.

Claims

1. The compound botanical medicine for treating chronic prostatitis and prostatic hyperplasia is characterized by comprising the following components in parts by weight: 12-18 parts of yam powder, 8-10 parts of leek seed powder, 8-10 parts of cranberry powder, 12-15 parts of black ginger extract, 5-8 parts of cinnamon extract, 3-5 parts of morinda officinalis, 3-5 parts of poria cocos, 5-8 parts of liquorice, 5-8 parts of lithospermum, 8-10 parts of rheum officinale, 12-15 parts of pumpkin seed powder, 0.1-0.2 part of calcium lactate, 5-8 parts of selenium-enriched protein powder, 2-3 parts of tartaric acid, 3-5 parts of gardenia jasminoides, 0.8-1.2 parts of xylan and 10-12 parts of soluble starch.

2. The compound botanical drug as claimed in claim 1, wherein the preparation method of cranberry powder comprises:

s15, mixing the second extract with the first extract, and filtering by a microfiltration ceramic membrane, wherein the operation temperature is controlled to be 55-65 ℃ to obtain microfiltration membrane permeate; filtering the microfiltration membrane permeate through a roll-type ultrafiltration membrane, and controlling the operation temperature to be 55-65 ℃ to obtain ultrafiltration membrane permeate; concentrating the ultrafiltration membrane retentate through a roll-type high-pressure reverse osmosis membrane, and controlling the operation temperature to be 30-40 ℃ to obtain a concentrated solution; and (4) freeze-drying the concentrated solution, and crushing and sieving to obtain the cranberry powder.

3. The compound botanical drug as claimed in claim 1, wherein the preparation method of the black ginger extract comprises the following steps:

4. The compound botanical drug as claimed in claim 1, wherein the preparation method of the cinnamon extract comprises:

(2) performing supercritical carbon dioxide extraction on the cinnamon powder, wherein the extraction pressure is 25Mpa, the extraction temperature is 35 ℃, and CO is added₂The flow rate is 20L/h, and the extraction time is 3-5 hours; introducing 95% ethanol as carrying agent in an amount of 2-3% of the weight of the cinnamon powder, and extracting for 1 hour; then the mixture is subjected to secondary pressure reductionSeparating under the conditions of primary pressure reduction and separation pressure of 7Mpa and temperature of 45 deg.C, and secondary pressure reduction and separation pressure of 6Mpa and temperature of 40 deg.C to obtain cortex Cinnamomi active substance;

(6) filtering the clear liquid by a microfiltration ceramic membrane, and controlling the operation temperature to be 55-65 ℃ to obtain microfiltration membrane permeate; filtering the microfiltration membrane permeate through a roll-type ultrafiltration membrane, and controlling the operation temperature to be 55-65 ℃ to obtain ultrafiltration membrane permeate; concentrating the ultrafiltration membrane retentate through a roll-type high-pressure reverse osmosis membrane, and controlling the operation temperature to be 30-40 ℃ to obtain a concentrated solution; the concentrated solution is the cinnamon extract.

5. The compound botanical of claim 4, wherein the buffer is a phosphate buffer.

6. The compound botanical drug as claimed in claim 4, wherein the first enzymolysis and/or the second enzymolysis and/or the third enzymolysis is carried out while the ultrasonic treatment is carried out, the ultrasonic power is 200-.

7. The preparation method of the compound botanical medicine for treating chronic prostatitis and prostatic hyperplasia is characterized by comprising the following steps:

s200, fully mixing the morinda officinalis, the poria cocos, the liquorice, the lithospermum, the rheum officinale and the gardenia jasminoides according to the weight portion of the claim 1 to obtain a raw material mixture, putting the raw material mixture into a primary fermentation tank, adding deionized water in an amount which is 3-5 times of the weight of the raw material mixture and cellulase in an amount which is 0.3 time of the weight of the raw material mixture, adjusting the pH value to 6.8-7, adjusting the temperature to 30 ℃ for primary fermentation, and separating a liquid first fermentation liquid after fermentation for 36 hours; after the first fermentation liquid is separated out, adding deionized water with the weight 0.8 time of that of the residue in the primary fermentation tank and pectinase with the weight 0.2 time of that of the residue in the primary fermentation tank into the residue in the primary fermentation tank, adjusting the pH to 4.0, adjusting the temperature to 45 ℃, performing primary fermentation again, and separating out liquid second fermentation liquid after fermentation for 24 hours; combining the first fermentation liquor and the second fermentation liquor to obtain combined fermentation liquor, centrifuging the combined fermentation liquor, and removing precipitates to obtain fermentation clear liquid;