CN110772628A

CN110772628A - Composite nutrient for promoting blood circulation to remove blood stasis and resisting prostatitis and hyperplasia and preparation method thereof

Info

Publication number: CN110772628A
Application number: CN201911020608.XA
Authority: CN
Inventors: 何静仁; 李玉保
Original assignee: Yunhong Group Co Ltd
Current assignee: Yunhong Group Co Ltd
Priority date: 2019-10-25
Filing date: 2019-10-25
Publication date: 2020-02-11

Abstract

The invention discloses a compound nutrient for promoting blood circulation to remove blood stasis and resisting prostatitis and hyperplasia and a preparation method thereof, wherein the compound nutrient comprises the following components: 10-15 parts of roselle, 5-8 parts of pseudo-ginseng flower, 15-20 parts of black ginger extract, 8-10 parts of cinnamon extract, 10-12 parts of cranberry, 5-8 parts of dandelion, 5-8 parts of honeysuckle, 3-5 parts of houttuynia cordata, 5-8 parts of corn stigma, 8-10 parts of lotus leaf, 10-15 parts of bitter gourd polypeptide powder, 0.2-0.3 part of tricalcium phosphate, 1-2 parts of citric acid, 3-5 parts of bixin, 1-2 parts of maltodextrin, 0.5-1 part of lactose and 5-8 parts of soluble starch. It can reduce pigment content in the black ginger extract, improve appearance, and increase yield and purity of active ingredients in the black ginger extract and the cinnamon extract, so as to achieve the effects of clearing away heat and toxic materials, promoting blood circulation, dredging channels, resisting bacteria, diminishing inflammation, enhancing immunity, etc., and thus can be used for preventing or adjunctively treating prostatitis and hyperplasia.

Description

Composite nutrient for promoting blood circulation to remove blood stasis and resisting prostatitis and hyperplasia and preparation method thereof

Technical Field

The invention relates to the field of health-care food. More specifically, the invention relates to a composite nutrient for promoting blood circulation to remove blood stasis and resisting prostatitis and hyperplasia and a preparation method thereof.

Background

Prostatitis refers to a prostate disease caused by various complex causes and mainly clinically manifested by urethral irritation symptoms and chronic pelvic pain, and the prostatitis accounts for the first place in male patients under 50 years old of urology surgery. Although the incidence of prostatitis is high, the cause of prostatitis is still unclear, especially nonbacterial prostatitis, and thus the treatment is mainly for improving symptoms.

At present, prostatitis patients are mostly treated by antibiotics, and long-term use of the antibiotics can cause drug resistance and reduce the immunity of organisms. Therefore, how to develop a health product for preventing prostatitis has been receiving more and more attention in recent years.

For example, the Chinese invention patent application number 201210171048.X discloses a medicine or health food composition for preventing or treating prostate diseases, which is a preparation prepared from the following raw materials in parts by weight: 5-9 parts of radish and 1-4 parts of honey. The invention also provides a preparation method and application of the composition. However, the radish juice of the raw product in the invention has the braised spicy taste and poor taste.

Therefore, it is important to provide a health product/medicine with significant effect of relieving prostatitis symptoms, so that patients can get rid of taking antibiotics.

Disclosure of Invention

In order to solve the technical problems, the invention provides a composite nutrient for promoting blood circulation to remove blood stasis and resisting prostatitis and hyperplasia and a preparation method thereof, the black ginger extract and the cinnamon extract are prepared by a special preparation process, the pigment content in the black ginger extract is reduced, the appearance and the taste are improved, the yield and the purity of active ingredients in the black ginger extract and the cinnamon extract are improved, and the effects of clearing heat and removing toxicity, promoting blood circulation to restore menstrual flow, resisting bacteria and diminishing inflammation, enhancing immunity and the like are further realized by reasonable compatibility with other components, so that the composite nutrient can be used for preventing or assisting in treating prostatitis and hyperplasia.

To achieve these objects and other advantages in accordance with the present invention, there is provided a compound nutrient for promoting blood circulation to remove blood stasis and resisting prostatitis and hyperplasia, comprising in parts by weight: 10-15 parts of roselle, 5-8 parts of pseudo-ginseng flower, 15-20 parts of black ginger extract, 8-10 parts of cinnamon extract, 10-12 parts of cranberry, 5-8 parts of dandelion, 5-8 parts of honeysuckle, 3-5 parts of houttuynia cordata, 5-8 parts of corn stigma, 8-10 parts of lotus leaf, 10-15 parts of bitter gourd polypeptide powder, 0.2-0.3 part of tricalcium phosphate, 1-2 parts of citric acid, 3-5 parts of bixin, 1-2 parts of maltodextrin, 0.5-1 part of lactose and 5-8 parts of soluble starch.

Preferably, the preparation method of the momordica charantia peptide powder comprises the following steps:

s11, taking one or more of fresh bitter gourds, dried bitter gourds and bitter gourds as bitter gourds, adding deionized water with the weight 5 times of that of the bitter gourds, soaking for 10-12 hours at the water temperature of 25 ℃, taking out and washing for 2-3 times by using the deionized water;

s12, drying the washed bitter gourd raw materials, smashing and grinding the bitter gourd raw materials into pulp to obtain bitter gourd pulp;

s13, taking the balsam pear pulp and the buffer solution to mix so as to obtain a mixed system, wherein the weight ratio of the balsam pear pulp is as follows: 1 (3-5) of buffer solution; recording the total volume value of the mixed system, adjusting the pH value to 6.8-7, and then carrying out temperature treatment on the mixed system to obtain an extract;

the temperature treatment process comprises the following steps:

heating to 45-55 ℃, preserving heat for 45-60min, cooling to 20-25 ℃, preserving heat for 25-30min, and recording the first volume value of the whole mixed system; a first mixed solution containing deionized water and a buffer was supplemented at 60% (total volume-first volume), and the deionized water: buffer 4: 1; after the first mixed solution is added, heating to 60-75 ℃, preserving heat for 60-75min, then cooling to 45-55 ℃, preserving heat for 30-35min, and recording a second volume value of the whole mixed system at the moment; a second mixed solution containing deionized water and a buffer was supplemented by 75% (total volume-second volume), and the deionized water: buffer 3: 1; adding the second mixed solution, heating to 80-90 deg.C, maintaining the temperature for 75-85min, cooling to 60-75 deg.C, and maintaining the temperature for 35-45 min;

s14, reducing the temperature of the extract to 20-25 ℃, and then carrying out enzymolysis on the extract to obtain a momordica charantia peptidase hydrolysis system; wherein, the enzymolysis process comprises the following steps:

carrying out first enzymolysis: adjusting the pH value of the extract to 7.5-8.5, adding trypsin according to 5% of the weight of the extract, stirring at 80-100 r/min, heating to 35-40 ℃ while stirring, and preserving heat for 45-60min to obtain a first enzymolysis system;

and (3) carrying out second enzymolysis: after the temperature of the first enzymolysis system is reduced to 20-25 ℃, adjusting the pH value to 3.0-4.0, adding pectinase according to 3% of the weight of the first enzymolysis system, stirring at 80-100 r/min, heating to 45-55 ℃ while stirring, and preserving heat for 40-60min to obtain a second enzymolysis system;

and (3) carrying out third enzymolysis: after the temperature of the second enzymolysis system is reduced to 20-25 ℃, adjusting the pH value to 4.5-5.0, adding cellulase according to 2% of the weight of the second enzymolysis system, stirring at 80-100 r/min, heating to 55-60 ℃ while stirring, and preserving heat for 30-45min to obtain a third enzymolysis system;

s15, after the enzymolysis process in the step S14 is finished, heating the obtained third enzymolysis system to 90 ℃, and maintaining for 10min to finish the enzyme deactivation process to obtain a bitter gourd peptide crude extraction system; adding activated carbon in the crude extract system of the bitter gourd peptide according to 4-5% of the weight of the bitter gourd peptide, uniformly stirring, keeping the temperature at 65 ℃ for 60-90min, centrifuging, and removing sediments to obtain a crude extract of the bitter gourd peptide;

filtering the crude extract of the bitter gourd peptide by diatomite to obtain a bitter gourd peptide clear solution, wherein the filtering pressure is 0.2-0.3 MPa; adding 4-5% of active carbon into the bitter gourd peptide clear liquid by weight, standing for 45-50min, centrifuging, and removing sediments;

s16, filtering the bitter gourd peptide clear liquid after removing the sediment by a microfiltration ceramic membrane with the filtering aperture of 0.5-0.8 mu m, wherein the filtering temperature is 55-65 ℃ to obtain microfiltration membrane permeate;

filtering the microfiltration membrane permeate through a 200kDa roll-type ultrafiltration membrane with the molecular weight cutoff of 100-;

concentrating the ultrafiltration membrane retentate through a roll-type high-pressure reverse osmosis membrane with the molecular weight cutoff of 150-;

s17, drying the momordica charantia peptide concentrated solution by a vacuum freeze drying method to obtain the momordica charantia polypeptide protein

Fructus Momordicae Charantiae peptide powder with content of not less than 30%.

Preferably, the preparation method of the black ginger extract comprises the following steps:

s21, soaking the black ginger at 25 ℃ for 10-12h, taking out, washing with deionized water for 2-3 times, drying, grinding, and sieving with a 100-mesh sieve to obtain black ginger powder;

s22, performing supercritical carbon dioxide extraction on the black ginger powder, wherein the extraction pressure is 30Mpa, the extraction temperature is 37 ℃, the CO2 flow rate is 25L/h, and the extraction time is 3-5 hours; introducing 95% ethanol with the weight of 3-5% of the weight of the black ginger powder as a carrying agent, and extracting for 1.5 hours; performing secondary pressure reduction separation at 45 deg.C under 8Mpa and 7Mpa to obtain liposoluble active substances of black rhizoma Zingiberis recens powder;

s23, taking the fat-soluble active substance of the black ginger powder, adding deionized water with the weight 5-10 times of that of the fat-soluble active substance of the black ginger powder to obtain an enzymolysis raw material, and carrying out enzymolysis on the enzymolysis raw material to obtain an enzymolysis system; wherein, the enzymolysis process comprises the following steps:

carrying out first enzymolysis: adjusting the pH value of the enzymolysis raw material to 8.5, adding trypsin according to 2-3% of the weight of the enzymolysis raw material, fully stirring, heating to 42 ℃ while stirring, and preserving heat for 35-40min to obtain a first enzymolysis system;

and (3) carrying out second enzymolysis: after the temperature of the first enzymolysis system is reduced to 20-25 ℃, adjusting the pH value to 4.0, adding pectinase according to 4 percent of the weight of the first enzymolysis system, fully stirring, heating to 45 ℃ while stirring, and preserving heat for 30-35min to obtain a second enzymolysis system;

and (3) carrying out third enzymolysis: after the temperature of the second enzymolysis system is reduced to 20-25 ℃, adjusting the pH value to 5.0, adding cellulase according to 3% of the weight of the second enzymolysis system, fully stirring, heating to 60 ℃ while stirring, and preserving heat for 25-35min to obtain a third enzymolysis system;

s24, after the enzymolysis is finished, heating the obtained third enzymolysis system to 85 ℃, and maintaining for 10min to finish the enzyme deactivation process;

s25, loading the resin into a column by using macroporous adsorption resin, washing the resin by using pure water, a sodium hydroxide solution with the mass fraction of 5% and a hydrochloric acid solution with the volume fraction of 5%, and loading a third enzymolysis system which is 8-10 times of the column volume and finishes enzyme deactivation on the column after the washing treatment is finished; washing the column with 5% by volume of 1.5-2 column volumes of hydrochloric acid, and washing with pure water to pH 6.8-7.0; eluting with 60% acetone, and collecting eluate; concentrating the eluent under reduced pressure at 50 deg.C until all acetone is removed; adjusting the pH value of the eluent without the acetone to 5.5-6.5, adding petroleum ether with the volume 1 time that of the eluent for extraction, removing a lower liquid phase containing pigment, and adding water-saturated n-butyl alcohol into an upper liquid phase for extraction for 3 times to remove water-soluble impurities, wherein the water-saturated n-butyl alcohol is calculated according to the volume ratio: the upper liquid phase is 2.5: 1; recovering water saturated n-butanol to obtain rhizoma Zingiberis recens powder extractive solution;

s26, adding activated carbon 4% of the weight of the black ginger powder extracting solution, stirring uniformly, keeping the temperature at 65 ℃ for 65-85min, centrifuging, filtering the liquid phase after removing the sediment through diatomite to obtain a refined black ginger powder extracting solution, wherein the filtering pressure is controlled at 0.25-0.35 MPa;

s27, concentrating the refined black ginger powder extract until the refined black ginger powder extract is dried to obtain a crude crystal; dissolving the obtained crude crystal with anhydrous ethanol, cooling, recrystallizing at 4-5 deg.C, and vacuum filtering after 12 hr to obtain black rhizoma Zingiberis recens extract crystal; repeating the steps of dissolving with absolute ethyl alcohol and recrystallizing for 2-3 times; and finally drying and crushing the crystals to obtain the black ginger extract.

Preferably, the preparation method of the cinnamon extract comprises the following steps:

(1) weighing cinnamon raw materials, soaking for 24-36h, washing with deionized water for 2-3 times, drying, crushing, and sieving with a 100-mesh sieve to obtain cinnamon powder;

(2) performing supercritical carbon dioxide extraction on the cinnamon powder, wherein the extraction pressure is 25Mpa, the extraction temperature is 35 ℃, the CO2 flow rate is 20L/h, and the extraction time is 3-5 hours; introducing 95% ethanol as carrying agent in an amount of 2-3% of the weight of the cinnamon powder, and extracting for 1 hour; performing secondary pressure reduction separation at 45 deg.C under 7Mpa and 6Mpa to obtain cortex Cinnamomi active substance;

(3) mixing the cinnamon active substance, a buffer solution and deionized water, wherein the raw material powder comprises the following components in percentage by weight: buffer solution: deionized water ═ 1 (3-4): (10-15) to obtain a mixed system, recording the total volume value of the mixed system, and adjusting the pH value to 6.8-7; heating the mixed system to 50-60 ℃, and keeping the temperature for 60-75min to obtain an extraction system;

(3) carrying out enzymolysis on the leaching system to obtain an enzymolysis system; wherein, the enzymolysis process comprises the following steps:

carrying out first enzymolysis: adjusting the pH value of the leaching system to 8.0-9.0, adding trypsin according to 4% of the weight of the leaching system, fully stirring, heating to 50-55 ℃ while stirring, and preserving heat for 30-35min to obtain a first enzymolysis system;

and (3) carrying out second enzymolysis: cooling the first enzymolysis system to 20-25 deg.C, adjusting pH to 3.0-4.0, adding pectase 5% of the first enzymolysis system, stirring, heating to 40-45 deg.C while stirring, and maintaining the temperature for 30-35min to obtain a second enzymolysis system;

and (3) carrying out third enzymolysis: after the temperature of the second enzymolysis system is reduced to 20-25 ℃, adjusting the pH value to 4.5-5.0, adding cellulase according to 2% of the weight of the second enzymolysis system, fully stirring, heating to 50-65 ℃ while stirring, and preserving heat for 20-30min to obtain a third enzymolysis system;

(4) loading the column with macroporous adsorption resin, washing the resin with pure water, 5% by mass of sodium hydroxide solution and 5% by volume of hydrochloric acid solution, and loading a third enzymolysis system which is 5-6 times the column volume and completes enzyme deactivation on the column; washing the column with 1.5-2 column volumes of 5% hydrochloric acid, and washing with pure water to pH 6.8-7.0; eluting with 60% acetone, and collecting eluate; concentrating the eluent under reduced pressure at 50 deg.C until all acetone is removed; adjusting the pH value of the eluent without the acetone to 5.5-6.5, adding petroleum ether with the volume 0.8 times of that of the eluent for extraction, removing a lower liquid phase containing pigment, and adding water-saturated n-butyl alcohol into an upper liquid phase for extraction for 3 times to remove water-soluble impurities, wherein the water-saturated n-butyl alcohol is calculated according to the volume ratio: the upper liquid phase is 2: 1; recovering water saturated n-butanol to obtain cortex Cinnamomi extractive solution;

(5) adding activated carbon into the cinnamon extracting solution according to 3% of the weight of the cinnamon extracting solution, uniformly stirring, preserving the heat at 65 ℃ for 60-90min, centrifuging, and removing sediments to obtain clear liquid;

(6) filtering the clear liquid with microfiltration ceramic membrane at 55-65 deg.C to obtain microfiltration membrane permeate

Liquid; filtering the microfiltration membrane permeate through a roll-type ultrafiltration membrane, and controlling the operation temperature to be 55-65 ℃ to obtain ultrafiltration membrane permeate; concentrating the ultrafiltration membrane retentate through a roll-type high-pressure reverse osmosis membrane, and controlling the operation temperature to be 30-40 ℃ to obtain a concentrated solution; the concentrated solution is the cinnamon extract.

Preferably, the buffer is a phosphate buffer.

Preferably, the first enzymolysis and/or the second enzymolysis and/or the third enzymolysis are carried out while ultrasonic treatment is carried out, wherein the ultrasonic power is 200- & lt 400 & gt W, and the ultrasonic treatment time is 10-15 min.

Also provides a preparation method of the compound nutrient for promoting blood circulation to remove blood stasis and resisting prostatitis and hyperplasia, which comprises the following steps:

s100, preparing the bitter gourd polypeptide powder, the black ginger extract and the cinnamon extract;

s200, weighing and mixing the roselle, the notoginseng flower, the black ginger extract, the cinnamon extract, the cranberry, the dandelion, the honeysuckle flower, the houttuynia cordata, the corn stigma, the lotus leaf, the bitter gourd polypeptide powder and the annatto according to the parts by weight in the claim 1 to obtain a mixture, adding deionized water into the mixture while stirring for dissolving, and stirring at the rotating speed of 500-800 rpm; and adding tricalcium phosphate, citric acid, maltodextrin, lactose and soluble starch in the weight portions as defined in claim 1 while stirring to obtain a stock solution;

s400, concentrating the stock solution to a concentrated solution with the relative density of 1.0 +/-1.15 at 60 ℃, and then drying the concentrated solution under reduced pressure to obtain the collagen polypeptide compound nutrient for improving the bone density.

The invention at least comprises the following beneficial effects:

the invention makes the content of the balsam pear polypeptide protein in the balsam pear peptide not less than 30% by the extraction process of staged heating, repeated enzymolysis and multiple filtration, simultaneously removes the pigment content in the black ginger powder extract by the steps of supercritical carbon dioxide extraction, repeated enzymolysis, macroporous adsorption resin adsorption and the like, increases the content and purity of active substances (such as total polymethoxyflavone and the like), similarly, prepares the cinnamon extract by the repeated enzymolysis mode, makes the cell wall structure of the cinnamon destroyed, thereby being beneficial to the full separation of nutrient components in cells, further, the components can play the roles of clearing away heat and toxic materials, activating blood and stimulating the menstrual flow, resisting bacteria and diminishing inflammation, enhancing immunity and the like by the reasonable compatibility with other components, thereby being capable of preventing or assisting in treating prostatitis and hyperplasia.

Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.

Detailed Description

The present invention is further described in detail below with reference to examples to enable those skilled in the art to practice the invention with reference to the description.

It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.

It is to be noted that the test methods described in the following embodiments are conventional methods unless otherwise specified, and the reagents and materials are commercially available without otherwise specified.

< example 1>

The composite nutrient for promoting blood circulation to remove blood stasis and resisting prostatitis and hyperplasia comprises the following components in parts by weight: 10 parts of roselle, 5 parts of pseudo-ginseng flower, 15 parts of black ginger extract, 8 parts of cinnamon extract, 10 parts of cranberry, 5 parts of dandelion, 5 parts of honeysuckle, 3 parts of houttuynia cordata, 5 parts of corn stigma, 8 parts of lotus leaf, 10 parts of bitter gourd polypeptide powder, 0.2 part of tricalcium phosphate, 1 part of citric acid, 3 parts of bixin, 1 part of maltodextrin, 0.5 part of lactose and 5 parts of soluble starch.

Further, the preparation method of the bitter gourd peptide powder comprises the following steps:

s11, taking one or more of fresh bitter gourds, dried bitter gourds and bitter gourds as bitter gourds, adding deionized water with the weight 5 times of that of the bitter gourds, soaking for 10-12 hours (preferably 10.5 hours) at the temperature of 25 ℃, taking out, and washing for 2-3 times by using the deionized water to remove pesticide residues and impurities;

s12, drying the washed bitter gourd raw materials in air, taking out, smashing and grinding to obtain bitter gourd pulp;

s13, mixing the balsam pear pulp with a buffer solution (the buffer solution is a buffer system containing reagents such as acid, alkali, salt and the like, such as a phosphate buffer solution) to obtain a mixed system, wherein the weight ratio of the balsam pear pulp is as follows: buffer 1 (3-5) (preferably 1: 4); recording the total volume value of the mixed system, adjusting the pH value to 6.8-7, and then carrying out temperature treatment on the mixed system to obtain an extract;

wherein the temperature treatment process comprises:

heating to 45-55 ℃ (preferably 50 ℃), keeping the temperature for 45-60min (preferably 55min), cooling to 20-25 ℃ (preferably 22 ℃), keeping the temperature for 25-30min (preferably 28min), and recording the first volume value of the whole mixed system at the moment; since water, acid, etc. in the reaction system may be evaporated during the aforementioned temperature rising and holding process, which may cause the solubility of acid, alkali, and inorganic ions to change, thereby affecting the leaching effect, after the aforementioned temperature rising and holding process, a first mixed solution containing deionized water and the buffer solution is added according to (total volume — first volume) × 60%, and the deionized water is calculated according to the weight ratio: buffer 4: 1, compensating the reaction system after evaporation of water, acid and the like, so that the reaction system is always in a better leaching environment; adding the first mixed solution, heating to 60-75 ℃ (preferably 65 ℃), keeping the temperature for 60-75min (preferably 65min), cooling to 45-55 ℃ (preferably 50 ℃), keeping the temperature for 30-35min (preferably 32 ℃), and recording a second volume value of the whole mixed system at the moment; a second mixed solution containing deionized water and a buffer was supplemented by 75% (total volume-second volume), and the deionized water: buffer 3: 1, the temperature of the temperature rise and the heat preservation is increased compared with the first time, so that the evaporation effect of water, acid and the like in the reaction system is more obvious, the proportion of the second mixed solution supplemented at this time is increased (to 75%), and the proportion of the buffer solution in the second mixed solution is increased; adding the second mixture, heating to 80-90 deg.C (preferably 85 deg.C), maintaining the temperature for 75-85min (preferably 80min), cooling to 60-75 deg.C (preferably 70 deg.C), and maintaining the temperature for 35-45min (preferably 40 min);

in the step, cell structure (such as cell walls and the like) compositions of the components can be repeatedly impacted and destroyed in different temperature change environments through staged temperature rise and heat preservation, and meanwhile, water and buffer solution in corresponding proportion are supplemented after each temperature rise and heat preservation stage is finished, so that a reaction system after water, acid and the like are evaporated is compensated, the reaction system is always in a better leaching environment, and the best leaching effect is achieved;

carrying out first enzymolysis: adjusting pH of the extract to 7.5-8.5 (preferably 7.0), adding trypsin 5% of the extract, stirring at 80-100 rpm, heating to 35-40 deg.C (preferably 37 deg.C) while stirring, and maintaining for 45-60min (preferably 55min) to obtain a first enzymolysis system;

and (3) carrying out second enzymolysis: after the temperature of the first enzymolysis system is reduced to 20-25 ℃, adjusting the pH value to 3.0-4.0 (preferably 3.5), adding pectinase according to 3% of the weight of the first enzymolysis system, stirring at 80-100 r/min, heating to 45-55 ℃ (preferably 50 ℃) while stirring, and keeping the temperature for 40-60min (preferably 50min) to obtain a second enzymolysis system;

and (3) carrying out third enzymolysis: after the temperature of the second enzymolysis system is reduced to 20-25 ℃, adjusting the pH value to 4.5-5.0 (preferably 4.7), adding cellulase according to 2% of the weight of the second enzymolysis system, stirring at 80-100 r/min, heating to 55-60 ℃ while stirring (preferably 58 ℃), and keeping the temperature for 30-45min (preferably 35min) to obtain a third enzymolysis system;

in the invention, the components are plant components, and the cell structure of the plant components contains cell walls, so that in the step, the cell walls are subjected to full enzymolysis by adopting different enzymes and enzymolysis conditions at different stages, so that cellulose, pectin and other components in the cell walls are completely destroyed, and effective components (such as balsam pear polypeptide protein) in the cell walls can be fully released, thereby improving the extraction efficiency;

s15, after the enzymolysis process in the step S14 is finished, heating the obtained third enzymolysis system to 90 ℃, and maintaining for 10min to finish the enzyme deactivation process to obtain a bitter gourd peptide crude extraction system; adding activated carbon in the crude extract system according to 4-5% of the weight of the crude extract system, stirring uniformly, keeping the temperature at 65 ℃ for 60-90min (preferably 75min), centrifuging, and removing residues to obtain a crude extract of the bitter gourd peptide;

filtering the crude extract with diatomaceous earth to obtain fructus Momordicae Charantiae peptide clear solution, with filtering pressure of 0.2-0.3MPa (preferably 0.25 MPa); adding 4-5% of active carbon into the bitter gourd peptide clear liquid by weight, standing for 45-50min, centrifuging, and removing sediments;

through the adsorption treatment of the active carbon and the diatomite, the impurities such as pigment, suspended particles, colloid and the like in the momordica charantia peptidase hydrolyzed liquid ensure that the finally obtained finished product has higher purity;

s16, filtering the bitter gourd peptide clear liquid after removing the sediment by a microfiltration ceramic membrane with the filtering aperture of 0.5-0.8 μm, wherein the filtering temperature is 55-65 ℃ (preferably 60 ℃) to obtain microfiltration membrane permeate; furthermore, the microfiltration ceramic membrane adopts three membranes which are used in parallel;

filtering the microfiltration membrane permeate through a 200kDa roll-type ultrafiltration membrane with the molecular weight cutoff of 100-; wherein the roll-type ultrafiltration membrane is a roll-type ultrafiltration membrane with the molecular weight cutoff of 100-200kDa, and the roll-type ultrafiltration membrane adopts two membranes which are used in parallel;

concentrating the ultrafiltration membrane retentate through a roll-type high-pressure reverse osmosis membrane with the molecular weight cutoff of 150-; the roll-type high-pressure reverse osmosis membrane system is a high-pressure concentration membrane, is specifically made of composite material membranes such as (PS) polysulfone or (PFS) polyethersulfone materials and is used by connecting four membranes in series;

in the step, the bitter gourd polypeptide protein is separated and purified by adopting a multi-layer membrane separation and purification technology, the concentration temperature is low, and the natural activity and high content of the bitter gourd polypeptide are effectively ensured;

s17, drying the bitter gourd peptide concentrated solution by a vacuum freeze drying method to obtain bitter gourd peptide powder with the bitter gourd polypeptide protein content not less than 30%.

Meanwhile, the black ginger has the effects of nourishing and strengthening, enhancing energy, reducing blood sugar, recovering physical strength, improving circulation, improving dyspepsia and the like; the main special component is the multi-methoxyl flavonoid, and the effects of resisting oxidation, dilating blood vessels, resisting allergy, resisting obesity and preventing aging are achieved. Therefore, the present invention also provides a method for preparing a black ginger extract, which comprises the following steps:

s21, soaking the black ginger at 25 ℃ for 10-12h (preferably 11h), taking out, washing with deionized water for 2-3 times, drying, grinding, and sieving with a 100-mesh sieve to obtain black ginger powder;

s22, performing supercritical carbon dioxide extraction on the black ginger powder, wherein the extraction pressure is 30Mpa, the extraction temperature is 37 ℃, and CO is adopted ₂The flow rate is 25L/h, and the extraction time is 3-5 hours; introducing 95% ethanol with the weight of 3-5% of the weight of the black ginger powder as a carrying agent, and extracting for 1.5 hours; performing secondary pressure reduction separation at 45 deg.C under 8Mpa and 7Mpa to obtain liposoluble active substances of black rhizoma Zingiberis recens powder;

s23, taking the fat-soluble active substance of the black ginger powder, adding deionized water with the weight 5-10 times (preferably 8 times) of that of the fat-soluble active substance to obtain an enzymolysis raw material, and carrying out enzymolysis on the enzymolysis raw material to obtain an enzymolysis system; wherein, the enzymolysis process comprises the following steps:

and (3) carrying out third enzymolysis: after the temperature of the second enzymolysis system is reduced to normal temperature, adjusting the pH value to 5.0, adding cellulase according to 3% of the weight of the second enzymolysis system, fully stirring, heating to 60 ℃ while stirring, and preserving heat for 25-35min to obtain a third enzymolysis system;

s25, packing columns (phi 10cm multiplied by 120cm) with HZ816 macroporous adsorption resin, washing the resin with pure water, a sodium hydroxide solution with the mass fraction of 5% and a hydrochloric acid solution with the volume fraction of 5%, and loading a third enzymolysis system which is 8-10 times (preferably 9 times) the column volume and completes enzyme inactivation on the columns after the washing treatment is finished; washing the column with 5% hydrochloric acid of 1.5-2 column volumes, and washing with pure water to pH 6.8-7.0; eluting with 60% acetone, and collecting eluate; concentrating the eluent under reduced pressure at 50 deg.C until all acetone is removed; adjusting the pH value of the eluent after acetone removal to 5.5-6.5 (preferably 6.0), adding petroleum ether with the volume 1 time that of the eluent for extraction, removing a lower liquid phase containing pigments, and adding water-saturated n-butyl alcohol into the upper liquid phase for extraction for 3 times to remove water-soluble impurities, wherein the water-saturated n-butyl alcohol: the upper liquid phase is 2.5: 1; recovering water saturated n-butanol to obtain rhizoma Zingiberis recens powder extractive solution;

s26, adding activated carbon 4% of the weight of the black ginger powder extract, stirring uniformly, keeping the temperature at 65 ℃ for 65-85min (preferably 75min), centrifuging, removing the sediment, and filtering the liquid phase with diatomite to obtain a refined black ginger powder extract, wherein the filtering pressure is controlled at 0.25-0.35MPa (preferably 0.3 MPa);

Because the polymethoxylated flavonoids in the black ginger are fat-soluble substances, and the black ginger contains the polymethoxylated flavonoids, large-polarity substances such as pectin, protein, flavonoid glycoside and the like, and small-polarity substances such as essential oil, fat-soluble pigments and the like, the difficulty of extraction and later separation and purification of the black ginger extract is undoubtedly increased, and the purple fat-soluble pigments influence the appearance of the product. By the preparation method in the embodiment, the content and purity of the polymethoxylated flavonoids in the black ginger can be greatly improved, and the content of the black purple pigment is obviously reduced, so that the extract has an acceptable appearance.

In addition, the cortex Cinnamomi extract can inhibit the expression of cyclooxygenase-2 and carbon monoxide synthase of RAW2647 cell cultured in vitro, has antiinflammatory effect, has certain inhibition effect on acute and chronic inflammatory reaction, has prevention effect on adjuvant arthritis, and can prevent systemic secondary symptoms. Therefore, the present embodiment also provides a method for preparing a cinnamon extract, comprising:

(1) weighing cinnamon raw materials, soaking for 24-36h, washing for 2-3 times by using deionized water, drying, crushing, and sieving by using a 100-mesh sieve to obtain raw material powder;

(2) performing supercritical carbon dioxide extraction on the cinnamon powder, wherein the extraction pressure is 25Mpa, the extraction temperature is 35 ℃, and CO is added ₂The flow rate is 20L/h, and the extraction time is 3-5 hours; introducing 95% ethanol as carrying agent in an amount of 2-3% of the weight of the cinnamon powder, and extracting for 1 hour; performing secondary pressure reduction separation at 45 deg.C under 7Mpa and 6Mpa to obtain cortex Cinnamomi active substance;

(2) mixing the cinnamon active substance, a buffer solution (such as phosphate buffer salt) and deionized water, wherein the raw material powder comprises the following components in percentage by weight: buffer solution: deionized water ═ 1 (3-4): (10-15) (preferably 1:3.5:12) to obtain a mixed system, recording the total volume value of the mixed system, and adjusting the pH value to 6.8-7; heating the mixed system to 50-60 deg.C (preferably 55 deg.C), and maintaining for 60-75min (preferably 65min) to obtain leaching system;

carrying out first enzymolysis: adjusting pH of the leaching system to 8.0-9.0 (preferably 8.5), adding trypsin 4% of the weight of the leaching system, stirring, heating to 50-55 deg.C (preferably 52 deg.C) while stirring, and maintaining the temperature for 30-35min to obtain a first enzymolysis system;

and (3) carrying out second enzymolysis: cooling the first enzymolysis system to 20-25 deg.C, adjusting pH to 3.0-4.0 (preferably 3.5), adding pectase 5% of the first enzymolysis system, stirring, heating to 40-45 deg.C, and keeping the temperature for 30-35min to obtain a second enzymolysis system;

and (3) carrying out third enzymolysis: after the temperature of the second enzymolysis system is reduced to 20-25 ℃, adjusting the pH value to 4.5-5.0 (preferably 4.7), adding cellulase according to 2% of the weight of the second enzymolysis system, fully stirring, heating to 50-65 ℃ (preferably 60 ℃) while stirring, and preserving the heat for 20-30min to obtain a third enzymolysis system;

in addition, in order to further increase the extraction efficiency, the ultrasonic treatment is carried out while the first enzymolysis and/or the second enzymolysis and/or the third enzymolysis is carried out, the ultrasonic power is 200-400W (preferably 300W), and the ultrasonic treatment time is 10-15min (preferably 12min), so that the cell wall/membrane structure is fully destroyed, and the content active ingredients are fully separated out;

(5) adding active carbon in the third enzymolysis system according to 3% of the third enzymolysis system, stirring well, keeping the temperature at 65 deg.C for 60-90min (preferably 75min), centrifuging, and removing the residue to obtain clear solution;

(6) filtering the clear solution with microfiltration ceramic membrane at 55-65 deg.C (preferably 60 deg.C) to obtain microfiltration membrane permeate; filtering the microfiltration membrane permeate through a roll-type ultrafiltration membrane, and controlling the operation temperature to be 55-65 ℃ (preferably 60 ℃) to obtain ultrafiltration membrane permeate; concentrating the ultrafiltration membrane retentate through a roll-type high-pressure reverse osmosis membrane, and controlling the operation temperature to be 30-40 ℃ (preferably 35 ℃) to obtain a concentrated solution; the concentrated solution is radix Puerariae extract/cortex Cinnamomi extract.

< example 2>

Compared with the embodiment 1, the compound nutrient for promoting blood circulation to remove blood stasis and resisting prostatitis and hyperplasia is different only in that the compound nutrient is composed of the following components: 15 parts of roselle, 8 parts of pseudo-ginseng flower, 20 parts of black ginger extract, 10 parts of cinnamon extract, 12 parts of cranberry, 8 parts of dandelion, 8 parts of honeysuckle, 5 parts of houttuynia cordata, 8 parts of corn stigma, 10 parts of lotus leaf, 15 parts of bitter gourd polypeptide powder, 0.3 part of tricalcium phosphate, 2 parts of citric acid, 5 parts of bixin, 2 parts of maltodextrin, 1 part of lactose and 8 parts of soluble starch.

< example 3>

Compared with the embodiment 1, the compound nutrient for promoting blood circulation to remove blood stasis and resisting prostatitis and hyperplasia is different only in that the compound nutrient is composed of the following components: 12 parts of roselle, 7 parts of pseudo-ginseng flower, 18 parts of black ginger extract, 9 parts of cinnamon extract, 11 parts of cranberry, 6 parts of dandelion, 7 parts of honeysuckle, 4 parts of houttuynia cordata, 7 parts of corn stigma, 9 parts of lotus leaf, 12 parts of bitter gourd polypeptide powder, 0.25 part of tricalcium phosphate, 1.5 parts of citric acid, 4 parts of bixin, 1.5 parts of maltodextrin, 0.8 part of lactose and 7 parts of soluble starch.

< measurement of molecular weight of Momordica charantia peptide >

The method of example 1 of the application No. 201710832199.8 ("a new method for producing momordica charantia polypeptide protein extract at low temperature throughout, momordica charantia polypeptide protein extract and its use") was used to extract momordica charantia peptide as comparative example 1, which was subjected to high performance gel filtration chromatography with momordica charantia peptide powder prepared by the method of preparing momordica charantia peptide powder of examples 1-3 of the present invention to obtain the molecular weight and distribution range of momordica charantia peptide, and the results are shown in table 1.

TABLE 1 molecular weight and distribution of bitter gourd peptides

Therefore, in the preparation method of the bitter gourd peptide powder, firstly, the cell structure (such as cell walls and the like) composition of the components can be repeatedly impacted and destroyed under different temperature change environments through staged heating and heat preservation, meanwhile, water and buffer solution in corresponding proportions are supplemented after each heating and heat preservation stage is finished, so that a reaction system after water, acid and the like are evaporated is compensated, the reaction system is always in a better extraction environment, and further, the bitter gourd polypeptide protein is prepared by performing staged heating, repeated enzymolysis and multi-level membrane separation and purification technology extraction processes, so that the content of the bitter gourd polypeptide protein in the obtained bitter gourd polypeptide extract is higher than 30%, and the extract product does not contain polypeptide proteins which are not derived from bitter gourd, such as soybean protein polypeptide and the like. As can be seen from Table 1, the proportion of the Momordica charantia polypeptide fragments in the range of 5000-7000Da prepared by the invention is close to 30%, and the Momordica charantia polypeptide fragments in the range of 5000-7000Da have good anti-inflammatory function besides the function of regulating blood sugar, so that the obtained Momordica charantia polypeptide extract has significant anti-inflammatory efficacy.

< measurement results of Black ginger extract >

Taking black ginger as a raw material, freezing, taking out, crushing and sieving to obtain black ginger powder; weighing black ginger powder according to a material-liquid ratio of 1: adding 100(g/mL) of alkyl pyridine bromide salt aqueous solution with the molar concentration of 1.0mol/L, performing microwave extraction for 10 minutes under the conditions of the microwave power of 300W and the temperature of 45 ℃, naturally cooling to 25 ℃, and collecting microwave extraction liquid; vacuum freeze drying the microwave extract to obtain microwave extract concentrate; performing column chromatography separation on the microwave extraction concentrate, eluting with ethyl acetate, and collecting ethyl acetate eluate; the ethyl acetate was removed and then freeze-dried under vacuum to obtain the anti-aging Heiguan ginger extract as comparative example 2. The results of examination of the extract of Zingiber nigrum L obtained by the preparation methods of examples 1 to 3 of the present invention are shown in Table 2, in order to obtain the yield, purity and appearance evaluation of total polymethoxylated flavones.

TABLE 2 Total polymethoxylated flavones content, purity and appearance of Hei ginger extract

As can be seen from Table 2, the content of total polymethoxylated flavones in the extract of Zingiber officinale Roscoe obtained by the preparation method of the present invention is significantly higher than that of comparative example 2, and the purity is as high as 86%, thus the preparation method of the present invention can effectively remove large polar substances such as pectin, protein, flavonoid glycoside, etc., and small polar substances such as essential oil, liposoluble pigment, etc., and simultaneously significantly reduce the content of black purple pigment, so that the extract has acceptable appearance.

< measurement results of cinnamon extract >

Taking cortex Cinnamomi Japonici fine powder, and adding supercritical CO ₂In a fluid extraction kettle; setting supercritical CO ₂The fluid extraction pressure is 100-350bar, and the extraction temperature is 35 ℃; extracting cortex Cinnamomi Japonici for 10 min; then setting CO ₂The dynamic extraction flow rate is 2.5L/min, and the cassia bark is dynamically extracted for 10 min; collecting the obtained supercritical CO ₂Fluid extract of CO ₂And (3) completely volatilizing to obtain an extract, adding anhydrous sodium sulfate to dehydrate, filtering and drying to obtain the cinnamon extract serving as a comparative example 3. It was examined with cinnamon extract prepared by the preparation method of the present invention in examples 1 to 3 to obtain cinnamaldehyde, cinnamic acid content, and the results are shown in table 3.

TABLE 3 Cinnamaldehyde, cinnamic acid content of cinnamon extract

As can be seen from table 3, the cinnamon extract obtained by the preparation method of the present invention has significantly higher cinnamaldehyde and cinnamic acid content than comparative example 3, and both cinnamaldehyde and cinnamic acid have the functions of inhibiting nitric oxide formation, anti-inflammation, anti-oxidation, dilating vascular smooth muscle, etc., so as to have the effects of activating blood stasis, anti-inflammation, relieving pain, resisting prostatitis and prostatic hyperplasia, etc.

< example 4>

The embodiment also provides the compound nutrient for promoting blood circulation to remove blood stasis and resisting prostatitis and hyperplasia as in any one of embodiments 1 to 3, which comprises the following steps:

s100, preparing the bitter gourd polypeptide powder, the black ginger extract and the cinnamon extract according to the preparation method of any one of the embodiments 1 to 3;

s200, weighing the roselle, the notoginseng flower, the black ginger extract, the cinnamon extract, the cranberry, the dandelion, the honeysuckle flower, the houttuynia cordata, the corn stigma, the lotus leaf, the bitter gourd polypeptide powder and the annatto according to the parts by weight in any one of the embodiments 1 to 3, mixing to obtain a mixture, adding deionized water into the mixture while stirring for dissolving, and stirring at the speed of 500-800rpm (preferably 650 rpm); and adding tricalcium phosphate, citric acid, maltodextrin, lactose and soluble starch in the weight parts described in any one of the embodiments 1 to 3 while stirring to obtain a stock solution;

< efficacy evaluation test >

Selecting a subject: 40 patients diagnosed with prostatic hyperplasia and prostatitis were selected and randomly divided into experimental groups and control groups. All patients were diagnosed with benign prostatic hyperplasia by digital rectal examination and B-ultrasound examination. The diagnosis of prostatitis is conformed; digital rectal exam can palpate the full prostate with mild tooth tenderness. Abnormally increased inflammatory cells were visible in the prostate fluid under reduced pressure, but no bacterial growth. Control group: the levofloxacin capsule is orally taken, 0.4 g/time and 1 time/d; treatment groups: the compound nutrient of the invention (hereinafter, all are 'compound nutrient') is given on the basis of a control group, 0.5g/kg, 1 time/d; both groups of patients were treated continuously for four weeks.

After four weeks, relevant index detection is carried out, and the results are shown in tables 4-6. index selection includes (1) detrusor contraction function, namely bladder residual urine volume, maximum urine flow rate and bladder detrusor energy storage, and (2) inflammatory factors, namely serum IL-1 β, IL-2 and IL-10, and double antibody sandwich ELISA determination is adopted.

Table 4 therapeutic effective rate of complex nutrients

Group of	Number of examples	Show effect	Is effective	Invalidation	Total effective rate
						Treatment group	20	14	4	2	80％
Control group	20	11	5	4	75％

As can be seen from Table 4, the effective rate of the treatment of the compound nutrient of the invention is as high as 80% compared with the control group.

TABLE 5 Effect of Complex Nutrients on detrusor contraction function

As can be seen from Table 5, the residual urine volume in the urinary bladder after treatment was decreased in both groups of patients compared to before treatment, but the residual urine volume in the treated group was significantly less than that in the control group; the maximum uroflow rate and bladder detrusor energy storage of the two groups were increased, and the treatment effect of the treatment group was better than that of the control group. As can be seen from the data in the table, the compound nutrient of the invention has certain relieving effect on the hyperplasia of prostate.

TABLE 6 Effect of Complex Nutrients on inflammatory factors

Compared with the IL-1 β -2 and IL-10 levels of two groups of patients, the three levels are obviously reduced, which shows that the level of inflammatory factors in serum is reduced, but IL-1 β, IL-2 and IL-10 in a treatment group are reduced by 42 percent, 31 percent and 39 percent respectively before and after treatment, which shows that the anti-inflammatory effect of the compound nutrient is obviously better than that of a control group.

In addition, 60 male rats are taken and randomly divided into a normal control group, a model control group and estradiol group, the composite nutrient high, medium and low dose groups of the invention comprise 10 compound nutrient groups, except the normal control group, other 5 groups remove bilateral testicles by operation, after 7d of postoperative feeding, after wound healing, 4mg/kg of testosterone propionate is injected subcutaneously every day for 30d continuously to induce prostatic hyperplasia, and 20ml/kg of distilled water is filled into the normal control group and the model control group. Estradiol injection is carried out on estradiol groups at the subcutaneous level, estradiol formate is injected at 2.5mg/kg, high, medium and low dosage groups are drenched with 5g, 2.5g and 1.5g of the compound nutrient every day for 30 days continuously, the compound nutrient is killed after stopping the injection, prostate compound leaves are taken and weighed, and the gland coefficient is calculated; glandular epithelial cell height and acinar cavity diameter were measured and the results are shown in table 7.

TABLE 7 Effect of Complex Nutrients on glandular coefficient, glandular cell height and glandular Cavity diameter

Group of	Gland coefficient (mg/g)	Glandular cavity diameter (mum)	Height of glandular cell (μm)
				Model control group	2.78±0.81	979.5±118.3	140.2±14.3
Normal control group	0.64±0.12	673.6±87.9	91.8±16.4
				Estradiol group	1.93±0.45	766.8±125.1	105.1±14.2
High dose group	2.03±0.24	835.1±82.8	119.6±9.1
				Middle dose group	2.29±0.43	851.6±144.6	133.6±8.4
Low dose group	2.38±0.47	898.3±161.8	136.6±13.6

The results in table 7 show that compared with the model control group, the hyperplasia of prostate tissues of rats in the normal control group, the estradiol group and the high-dose group is not obvious, and the difference between the groups has significant significance, so that the compound nutrient has an inhibition effect on the hyperplasia of prostate tissues induced by testosterone propionate, and is beneficial to treating prostatitis and hyperplasia.

It should be noted that the technical solutions in the above embodiments 1 to 4 can be arbitrarily combined, and the technical solutions obtained after the combination all belong to the protection scope of the present invention.

In conclusion, the balsam pear polypeptide protein content in the balsam pear peptide is not lower than 30% through the extraction processes of staged heating, repeated enzymolysis and multiple filtration, the pigment content in the black ginger powder extract is removed through the steps of supercritical carbon dioxide extraction, repeated enzymolysis, macroporous adsorption resin adsorption and the like, the content and the purity of active substances (such as total polymethoxyflavone and the like) are increased, similarly, the preparation of the cinnamon extract is carried out through the repeated enzymolysis mode, the cell wall structure of the cinnamon is damaged, the full separation of nutrient components in cells is facilitated, and further, the components can play roles of clearing heat and removing toxicity, activating blood and stimulating the menstrual flow, resisting bacteria and diminishing inflammation, enhancing immunity and the like through reasonable compatibility with other components, so that the balsam pear polypeptide can be used for preventing or assisting in treating prostatitis and hyperplasia.

The number of apparatuses and the scale of the process described herein are intended to simplify the description of the present invention. Applications, modifications and variations of the present invention will be apparent to those skilled in the art.

While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable to various fields of endeavor for which the invention may be embodied with additional modifications as would be readily apparent to those skilled in the art, and the invention is therefore not limited to the details given herein and to the examples shown and described without departing from the generic concept as defined by the claims and their equivalents.

Claims

1. The composite nutrient for promoting blood circulation to remove blood stasis and resisting prostatitis and hyperplasia is characterized by comprising the following components in parts by weight: 10-15 parts of roselle, 5-8 parts of pseudo-ginseng flower, 15-20 parts of black ginger extract, 8-10 parts of cinnamon extract, 10-12 parts of cranberry, 5-8 parts of dandelion, 5-8 parts of honeysuckle, 3-5 parts of houttuynia cordata, 5-8 parts of corn stigma, 8-10 parts of lotus leaf, 10-15 parts of bitter gourd polypeptide powder, 0.2-0.3 part of tricalcium phosphate, 1-2 parts of citric acid, 3-5 parts of bixin, 1-2 parts of maltodextrin, 0.5-1 part of lactose and 5-8 parts of soluble starch.

2. The complex nutrient of claim 1, wherein the preparation method of the momordica charantia peptide powder comprises the following steps:

the temperature treatment process comprises the following steps:

3. The complex nutrient of claim 1, wherein the black ginger extract is prepared by a method comprising the steps of:

4. The complex nutrient of claim 1, wherein the cinnamon extract is prepared by a method comprising:

(6) filtering the clear liquid by a microfiltration ceramic membrane, and controlling the operation temperature to be 55-65 ℃ to obtain microfiltration membrane permeate; filtering the microfiltration membrane permeate through a roll-type ultrafiltration membrane, and controlling the operation temperature to be 55-65 ℃ to obtain ultrafiltration membrane permeate; concentrating the ultrafiltration membrane retentate through a roll-type high-pressure reverse osmosis membrane, and controlling the operation temperature to be 30-40 ℃ to obtain a concentrated solution; the concentrated solution is the cinnamon extract.

5. The complex nutrient of claim 4, wherein the buffer is a phosphate buffer.

6. The compound nutrient of claim 4, wherein the first enzymolysis and/or the second enzymolysis and/or the third enzymolysis are carried out simultaneously with ultrasonic treatment, wherein the ultrasonic power is 200-.

7. The preparation method of the compound nutrient for promoting blood circulation to remove blood stasis and resisting prostatitis and hyperplasia is characterized by comprising the following steps: