CN1107044A - Method for making pearl liquid from pearl or nacreous layer powder - Google Patents
Method for making pearl liquid from pearl or nacreous layer powder Download PDFInfo
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- CN1107044A CN1107044A CN94119843A CN94119843A CN1107044A CN 1107044 A CN1107044 A CN 1107044A CN 94119843 A CN94119843 A CN 94119843A CN 94119843 A CN94119843 A CN 94119843A CN 1107044 A CN1107044 A CN 1107044A
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- margarita
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- layer powder
- pearl
- aqueous slkali
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Abstract
A pearl liquid preparation method is that an alkali solution is added to the pearl powder according to a 1:1 proportion, the mixing solution is hydrolyzed at the temperature of 100DEG C-130DEG C for 20-60 minutes, the deionized water and the organic acid with a concentration of 50 percent are added into the hydrolysate to carry out a decomposition reaction, the pH value of the solution is controlled in the range of 3-6, after 12-20 hours at a room temperature, the solution is heated to 70-90DEG C and filtered immediately to get a finished product. The method has the advantages of a simple process, an easy operation and no need of multiple filtering, pickling, concentrating, extracting, decoloring and container switching, which lead to a low production cost, the high natural component retention and more than 97 percent protein recovery rate.
Description
The present invention relates to a kind of preparation method of Margarita liquid.
Traditional Margarita liquid of producing adopts hydrochloric acid and sulphuric acid hydrolysis more.Because more with hydrochloric acid hydrolysis deacidification difficulty, the Margarita liquid trial-production method of introducing as 1977 7 phases " Chinese herbal medicine ", complex process not only, front and back are altogether through six operations, and deacidification is also not thorough.If with reuse acetone extract aminoacid behind the keratoprotein hydrochloric acid hydrolysis liquid concentrate drying, then deacidification is comparatively thorough, and as CN 1078891A, but the cost energy consumption is too many again.And excessive sulphuric acid can add calcium hydroxide and separates with hydrolyzed solution, so adopt comparatively general at present.But also there is following defective in sulphuric acid hydrolysis system Margarita liquid: 1, if remove mineral nitrogen salt with organic acid earlier, complex process, and the Margarita keratoprotein response rate is not high, as CN 88105369A, earlier organic acid and Margarita powder are carried out metathesis reaction, from obtaining suspension, separate cotton-shaped Margarita keratoprotein again, because the institute's albumen that obtains containing impurity is a lot of, must be through pickling ability hydrolyzable, hydrolysis post neutralization excess sulfuric acid, use activated carbon decolorizing, front and back also need five steps, and yield only reaches 50%.2, then technology is simpler if directly sulphuric acid and Margarita powder are reacted, as CN 1083362A disclose a kind of from the Margarita Bulbus Fritillariae Uninbracteatae method of extraction Margarita liquid, protein yield can reach 80%, but mineral nitrogen salt wherein can't utilize.3, above-mentioned two technologies are all used the sulphuric acid hydrolysis keratin, and neutralize unavoidable residual calcium sulfate, i.e. Gypsum Fibrosum in the solution with calcium hydroxide.Though the gypsum dissolution degree is little, it is different that pharmacological action and effects of Gypsum there is no, and Gypsum Fibrosum Great Cold is usually used in the hyperpyrexia that acute calenture causes, is not suitable for old and weak people, women and children and for health care.
Enzyme hydrolysis method is simple than acid-hydrolysis method, and as CN 1083363A, but owing to contain a large amount of mineral nitrogen salt in the Margarita powder, reaction is difficult to thoroughly, and protein yield is generally also below 50%, and mineral nitrogen salt still can't utilize.
The objective of the invention is to overcome acid hydrolysis and the existing defective of enzyme hydrolysis legal system Margarita liquid, provide a kind of easy to operate, production cost is low, and it is many that natural component keeps, and what protein recovery was high produces the method for full Margarita liquid with Margarita or Margarita layer powder.
Technical scheme of the present invention is: Margarita or Margarita layer powder are placed hermetic container, in 1: the ratio 1(weight/volume) is added aqueous slkali, the percentage composition ratio of keratinous protein is 0.5~1.5 in the percentage concentration of aqueous slkali and the Margarita powder: the 1(w/w), be heated then, hydrolysis is 20~60 minutes under 100~130 ℃ temperature, is cooled to room temperature; Add deionized water by the aqueous slkali amount (volume) of twice in above-mentioned gained hydrolysate, then, slowly adding concentration is 50%(weight) organic acid carry out metathesis reaction, the limit edged stirs, pH value is controlled at 3~6, at room temperature placed 12~20 hours, regulating pH value with organic acid once more is 3~6, be heated to 70~90 ℃, filtered while hot promptly obtains pearl crude liquid, in order to improve protein recovery, can use the deionized water wash filtering residue, cleaning mixture and above-mentioned filtrate are merged.
In the present invention, used aqueous slkali can be sodium hydroxide or potassium hydroxide solution, and used organic acid can be acetic acid or lactic acid.
The pearl crude liquid that the present invention is obtained suitably dilutes, and adds adjuvant and is full Margarita liquid finished product.To can obtain soluble pearl powder behind the pearl crude liquid vacuum drying.
The present invention compares with existing full Margarita liquid preparation method, and technology is simple, and is easy to operate, do not need multiple times of filtration, pickling midway, concentrate, extract, decolour and the conversion container, thereby production cost is low, and natural component keeps many, and protein recovery can reach more than 97%.
Below in conjunction with embodiment the present invention is described in more detail.
Embodiment 1:
At first, with 1000 gram Margarita keratoprotein content is that 1.51% Margarita layer powder places in the good reaction pot of airtight performance, adding 1000 ml concns is 2%(weight) sodium hydroxide solution, stir into pasty state and also sweep away the layer powder that sticks to pot wall with 50 ml deionized water, hydrolysis is 40 minutes under 120 ± 2 ℃ temperature, naturally cool to room temperature, in hydrolysate, add 2000 ml deionized water, slowly add 4400 milliliters of 50%(weight again) edible lactic acid, the limit edged stirs, the bubble that metathesis reaction is produced overflows, regulating pH value with lactic acid is 3.5, place after 20 hours, regulating pH value with lactic acid once more is 3.5, is heated to 85 ± 2 ℃ then, filtered while hot, with 450 ml deionized water washing filtering residue, merging filtrate and cleaning mixture obtain 8431 milliliters of light yellow clear and bright pearl crude liquids.
After measured, the pH value of stock solution is 3.5, and aminoacid, content of peptides are 1.76 mg/ml, and the Margarita keratoprotein response rate is 98.3%, and this stock solution is at room temperature placed more than 2 years and never degenerated.
Embodiment 2:
At first, with 1000 gram Margarita keratoprotein content is that 3.0% Margarita powder places in the good reaction pot of airtight performance, adding 1000 ml concns is 3%(weight) potassium hydroxide solution, the furnishing pasty state, sweep away the Margarita powder that sticks to pot wall with 50 ml deionized water, hydrolysis is 20 minutes under 110 ± 2 ℃ temperature, naturally cools to room temperature; Add 2000 ml deionized water in above-mentioned gained hydrolysate, slowly adding concentration again is 50%(weight) acetic acid, the limit edged stirs, prevent that bubble from overflowing, no longer take place until bubble, pH value is controlled at 4.5, at room temperature placed 12 hours, regulating pH value with acetic acid once more is 4.5, be heated to 75 ± 2 ℃ then, filtered while hot is with 450 ml deionized water washing filtering residue, merging filtrate and cleaning mixture obtain light yellow clear and bright pearl crude liquid.
Claims (3)
1, a kind ofly produce the method for full Margarita liquid, it is characterized in that with Margarita or Margarita layer powder:
A, Margarita or Margarita layer powder are placed hermetic container, ratio in 1: 1 (weight/volume) is added aqueous slkali, the percentage composition ratio of keratoprotein is 0.5~1.5: 1 (w/w) in the percentage concentration of aqueous slkali and the Margarita powder, be heated then, hydrolysis is 20~60 minutes under 100~130 ℃ temperature, be cooled to room temperature
B, the aqueous slkali amount (volume) by twice in above-mentioned gained hydrolysate add deionized water, the organic acid that then slowly adds concentration and be 50% (weight) carries out metathesis reaction, the limit edged stirs, pH value is controlled at 3~6, at room temperature placed 12~20 hours, regulating pH value with organic acid once more is 3~6, is heated to 70~90 ℃, filtered while hot promptly obtains pearl crude liquid.
2, according to claim 1ly produce the method for full Margarita liquid with Margarita or Margarita layer powder, it is characterized in that: used aqueous slkali can be sodium hydroxide or potassium hydroxide solution.
3, according to claim 1 and 2ly produce the method for full Margarita liquid with Margarita or Margarita layer powder, it is characterized in that: used organic acid is acetic acid or lactic acid.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN94119843A CN1065747C (en) | 1994-12-14 | 1994-12-14 | Method for making pearl liquid from pearl or nacreous layer powder |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN94119843A CN1065747C (en) | 1994-12-14 | 1994-12-14 | Method for making pearl liquid from pearl or nacreous layer powder |
Publications (2)
Publication Number | Publication Date |
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CN1107044A true CN1107044A (en) | 1995-08-23 |
CN1065747C CN1065747C (en) | 2001-05-16 |
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CN94119843A Expired - Fee Related CN1065747C (en) | 1994-12-14 | 1994-12-14 | Method for making pearl liquid from pearl or nacreous layer powder |
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CN (1) | CN1065747C (en) |
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1994
- 1994-12-14 CN CN94119843A patent/CN1065747C/en not_active Expired - Fee Related
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CN1065747C (en) | 2001-05-16 |
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