CN1065747C

CN1065747C - Method for making pearl liquid from pearl or nacreous layer powder

Info

Publication number: CN1065747C
Application number: CN94119843A
Authority: CN
Inventors: 卢济台; 罗萍萍; 卢丁
Original assignee: Individual
Current assignee: Individual
Priority date: 1994-12-14
Filing date: 1994-12-14
Publication date: 2001-05-16
Anticipated expiration: 2014-12-14
Also published as: CN1107044A

Abstract

The present invention relates to a preparation method of pearl liquid, which comprises the following steps: aqueous alkali is added into pearl powder according to the proportion of 1:1, and hydrolysis is carried out for 20 to 60 minutes under the temperature of 100 to 130 DEG C; de-ionized water and organic acid with the concentration of 50% are added into the hydrolyzate, and a double decomposition reaction is carried out; pH is from 3 to 6 by control, and a placing process is carried out for 12 to 20 hours under room temperature; temperature is heated to 70 to 90 DEG C, and heat filtration is carried out to obtain a finished product. The preparation method of pearl liquid has the advantages of simple technology and convenient operation and does not need multiple filtration, an acid washing process, concentration, extraction, decolorization and vessel conversion in midway. Therefore, the preparation method of pearl liquid has low production cost and can reserve a plurality of natural components, and albumins recovery rate can reach more than 97%.

Description

Produce the method for full Margarita liquid with Margarita or Margarita layer powder

The present invention relates to a kind of preparation method of Margarita liquid.

Traditional Margarita liquid of producing adopts hydrochloric acid and sulphuric acid hydrolysis more.Because more with hydrochloric acid hydrolysis deacidification difficulty, the Margarita liquid trial-production method of introducing as 1977 7 phases " Chinese herbal medicine ", complex process not only, front and back are altogether through six operations, and deacidification is also not thorough.If with reuse acetone extract aminoacid behind the keratoprotein hydrochloric acid hydrolysis liquid concentrate drying, then deacidification is comparatively thorough, and as CN1078891A, but the cost energy consumption is too many again.And excessive sulphuric acid can add calcium hydroxide and separates with hydrolyzed solution, so adopt comparatively general at present.But also there is following defective in sulphuric acid hydrolysis system Margarita liquid: 1. if remove mineral nitrogen salt with organic acid earlier, complex process, and the Margarita keratoprotein response rate is not high, as CN88105369A, earlier organic acid and Margarita powder are carried out metathesis reaction, from obtaining suspension, separate cotton-shaped Margarita keratoprotein again, because the institute's albumen that obtains containing impurity is a lot of, must be through pickling ability hydrolyzable, hydrolysis post neutralization excess sulfuric acid, use activated carbon decolorizing, front and back also need five steps, and yield only reaches 50%.2. then technology is simpler if directly sulphuric acid and Margarita powder are reacted, as CN1083362A disclose a kind of from the Margarita Bulbus Fritillariae Uninbracteatae method of extraction Margarita liquid, protein yield can reach 80%, but mineral nitrogen salt wherein can't utilize.3. above-mentioned two technologies are all used the sulphuric acid hydrolysis keratin, and neutralize unavoidable residual calcium sulfate, i.e. Gypsum Fibrosum in the solution with calcium hydroxide.Though the gypsum dissolution degree is little, pharmacological action there is no different with effects of Gypsum.Gypsum Fibrosum Great Cold is usually used in the hyperpyrexia that acute calenture causes, is not suitable for old and weak people, women and children and for health care.

Enzyme hydrolysis method is simple than acid-hydrolysis method, and as CN1083363A, but owing to contain a large amount of mineral nitrogen salt in the Margarita powder, reaction is difficult to thoroughly, and protein yield is generally also below 50%, and mineral nitrogen salt still can't utilize.

The objective of the invention is to overcome acid hydrolysis and the existing defective of enzyme hydrolysis legal system Margarita liquid, provide a kind of easy to operate, production cost is low, and it is many that natural component keeps, and what protein recovery was high produces the method for full Margarita liquid with Margarita or Margarita layer powder.

Technical scheme of the present invention is: Margarita or Margarita layer powder are placed hermetic container, ratio in 1: 1 (weight/volume) is added aqueous slkali, the percentage composition ratio of keratoprotein is 0.5～1.5: 1 (w/w) in the percentage concentration of aqueous slkali and the Margarita powder, be heated then, hydrolysis is 20～60 minutes under 100～130 ℃ temperature, is cooled to room temperature; Add deionized water by the aqueous slkali amount (volume) of twice in above-mentioned gained hydrolysate, then, the organic acid that slowly adds concentration and be 50% (weight) carries out metathesis reaction, the limit edged stirs, pH value being controlled at 3～6, at room temperature placing 12～20 hours, is 3～6 with organic acid for adjusting pH value once more, be heated to 70～90 ℃, filtered while hot promptly obtains pearl crude liquid, in order to improve protein recovery, can use the deionized water wash filtering residue, cleaning mixture and above-mentioned filtrate are merged.

In the present invention, used aqueous slkali can be sodium hydroxide or potassium hydroxide solution, and used organic acid can be acetic acid or lactic acid.

The pearl crude liquid that the present invention is obtained suitably dilutes, and adds adjuvant and is full Margarita liquid finished product.To can obtain soluble pearl powder behind the pearl crude liquid vacuum drying.

The present invention compares with existing full Margarita liquid preparation method, and technology is simple, and is easy to operate, do not need multiple times of filtration, pickling midway, concentrate, extract, decolour and the conversion container, thereby production cost is low, the natural component reservation is many, and protein recovery can reach more than 97%.

Below in conjunction with embodiment the present invention is described in more detail.

Embodiment 1:

At first, with 1000 gram Margarita keratoprotein content is that 1.51% Margarita layer powder places in the good reaction pot of airtight performance, add the sodium hydroxide solution that 1000 ml concns are 2% (weight), stir into pasty state and also sweep away the layer powder that sticks to pot wall with 50 ml deionized water, hydrolysis is 40 minutes under 120 ± 2 ℃ temperature, naturally cool to room temperature, in hydrolysate, add 2000 ml deionized water, the edible lactic acid that slowly adds 4400 milliliter 50% (weight) again, the limit edged stirs, and the bubble that its decomposition reaction is produced overflows, regulating pH value with lactic acid is 3.5, place after 20 hours, regulating pH value with lactic acid once more is 3.5, is heated to 85 ± 2 ℃ then, filtered while hot, with 450 ml deionized water washing filtering residue, merging filtrate and cleaning mixture obtain 8431 milliliters of light yellow clear and bright pearl crude liquids.

After measured, the pH value of stock solution is 3.5, and aminoacid, content of peptides are 1.76 mg/ml, and the Margarita keratoprotein response rate is 98.3%, and this stock solution is at room temperature placed more than 2 years and never degenerated.

Embodiment 2:

At first, with 1000 gram Margarita keratoprotein content is that 3.0% Margarita powder places in the good reaction pot of airtight performance, add the potassium hydroxide solution that 1000 ml concns are 3% (weight), the furnishing pasty state, sweep away the Margarita powder that sticks to pot wall with 50 ml deionized water, hydrolysis is 20 minutes under 110 ± 2 ℃ temperature, naturally cools to room temperature; Add 2000 ml deionized water in above-mentioned gained hydrolysate, slowly add the acetic acid that concentration is 50% (weight) again, the limit edged stirs, prevent that bubble from overflowing, no longer take place until bubble, pH value is controlled at 4.5, at room temperature placed 12 hours, regulating pH value with acetic acid once more is 4.5, be heated to 75 ± 2 ℃ then, filtered while hot is with 450 ml deionized water washing filtering residue, merging filtrate and cleaning mixture obtain light yellow clear and bright pearl crude liquid.

Claims

1. produce the method for full Margarita liquid with Margarita or Margarita layer powder for one kind, it is characterized in that:

A, Margarita or Margarita layer powder are placed hermetic container, ratio in 1: 1 (grams per milliliter) is added sodium hydroxide or potassium hydroxide solution, the percentage composition of keratoprotein is than being 0.5-1.5 in the percentage concentration of sodium hydroxide or potassium hydroxide solution and the Margarita powder: 1 (w/w), be heated then, under 100-130 ℃ temperature hydrolysis 20-60 minute, be cooled to room temperature;

B, sodium hydroxide or potassium hydroxide solution amount (volume) by twice in above-mentioned gained hydrolysate add deionized water, the organic acid that then slowly adds concentration and be 50% (weight) carries out metathesis reaction, the limit edged stirs, pH value is controlled at 3-6, at room temperature placing 12-20 hour, is 3-6 with organic acid for adjusting pH value once more, is heated to 70-90 ℃, filtered while hot promptly obtains pearl crude liquid.

2. according to claim 1ly produce the method for full Margarita liquid with Margarita or Margarita layer powder, it is characterized in that: used organic acid is acetic acid or lactic acid.