CN110699465B - Molecular marker for rapidly improving green shell rate of duck group and application - Google Patents
Molecular marker for rapidly improving green shell rate of duck group and application Download PDFInfo
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Abstract
The invention discloses a molecular marker for rapidly improving the green shell rate of a duck group and application thereof. The invention provides application of a substance for detecting whether 47418074 th deoxyribonucleotide on No. 4 chromosome in duck genome is A or G or A and G in identification of color traits of duck eggshells to be detected. The inventor discovers specific SNP by carrying out whole genome sequencing on a large number of duck samples and excavating SNP related to eggshell color. The screening of green shell/white shell characters of the laying duck can be realized based on the genotype of the SNP locus, the genotype of the green shell character is AA type and AG type, and the genotype of the white shell character is GG type. The invention can realize rapid purification of eggshell color, can reach a purification state only through first-generation breeding, and can realize early selection of eggshell color characters. The method can save production cost and accelerate genetic progress, better serves duck breeding, and has great economic application value and scientific research value.
Description
Technical Field
The invention relates to a molecular marker assisted selection technology in the technical field of animal genetic breeding, in particular to a molecular marker for rapidly improving the green shell rate of a duck group and application thereof.
Background
The egg duck market and the breeding scale in China develop rapidly, and the breeding variety, quantity and yield are all in the top of the world. The green-shell duck egg shell has high strength, is not easy to damage in processing and is popular with consumers, and the breeding of the green-shell duck strain is an important requirement of the industry.
Researches show that the color character of the eggshell of the poultry can be stably inherited, and the blue-shell character of a plurality of poultry strains is determined by a dominant single gene. In the breeding of poultry green-shell eggs, recessive genes are difficult to eliminate by selecting the phenotype of female poultry green-shell eggs, the male poultry genotype can be judged only by test crossing experiments and descendant determination, and the green-shell egg rate can be improved and fixed by multi-generation breeding work. If the control gene of the green-shell egg character is known, the expected breeding target can be achieved by first-generation selection by using molecular biotechnology.
The molecular marker assisted breeding technology is a new breeding technology formed in recent years and has been widely applied to breeding new varieties of animals and plants. Molecular markers closely related to the target traits can be obtained through whole genome association analysis, and the molecular markers are used for early selection of the target traits, so that breeding cost can be greatly saved, and genetic progress can be accelerated. However, no relevant molecular marker for screening the color of the duck egg shells exists at present, which greatly slows down the screening progress of the color of the duck egg shells.
Disclosure of Invention
The invention aims to provide a molecular marker for rapidly improving the green shell rate of duck groups and application thereof.
In a first aspect, the present invention claims the use of a substance for detecting whether the 47418074 th deoxyribonucleotide on chromosome 4 (i.e., the 507 th deoxyribonucleotide of SEQ ID No. 3) is A, G, or A and G in the genome of a duck in any of the following:
(A1) identifying the color character of the duck egg shell to be detected;
(A2) preparing a product for identifying the color character of the duck eggshell to be detected.
Further, the substance for detecting whether the 47418074 th deoxyribonucleotide on chromosome 4 (i.e. the 507 th deoxyribonucleotide of SEQ ID No. 3) in the duck genome is A or G or A and G can be a primer pair or a reagent or a kit containing the primer pair.
The primer pair consists of an upstream primer and a downstream primer; the upstream primer is designed according to the upstream sequence of 47418074 th deoxyribonucleotide (namely 507 th deoxyribonucleotide of SEQ ID No. 3) on the No. 4 chromosome in the duck genome; the downstream primer is designed according to the downstream sequence of 47418074 th deoxyribonucleotide (namely 507 th deoxyribonucleotide of SEQ ID No. 3) on the No. 4 chromosome in the duck genome.
Further, the forward primer may be (a1) or (a2) as follows, and the reverse primer may be (a3) or (a4) as follows:
(a1) DNA molecule shown in SEQ ID No. 1;
(a2) DNA molecule which is obtained by substituting SEQ ID No.1 by one or more nucleotides and has the same function as SEQ ID No. 1;
(a3) DNA molecule shown in SEQ ID No. 2;
(a4) a DNA molecule which is obtained by substituting SEQ ID No.2 by one or more nucleotides and has the same function as SEQ ID No. 2.
The kit may also include conventional reagents for PCR amplification. The kit may also include sequencing conventional reagents.
In a second aspect, the invention claims a method for identifying the color character of the eggshell of the duck to be detected.
The method for identifying the color character of the duck eggshell to be detected, which is claimed by the invention, can comprise the following steps:
(B1) detecting whether the 47418074 th deoxyribonucleotide (namely the 507 th deoxyribonucleotide of SEQ ID No. 3) on the No. 4 chromosome in the genome of the duck to be detected is A, G or A and G, and then determining the genotype according to the following steps:
if 47418074 th deoxyribonucleotide (namely 507 th deoxyribonucleotide of SEQ ID No. 3) on the No. 4 chromosome in the genome of the duck to be detected is a homozygote of A, the genotype of the duck to be detected is A: A;
if the 47418074 th deoxyribonucleotide (namely the 507 th deoxyribonucleotide of SEQ ID No. 3) on the No. 4 chromosome in the genome of the duck to be detected is a homozygote of G, the genotype of the duck to be detected is G: G type;
if 47418074 th deoxyribonucleotide (namely 507 th deoxyribonucleotide of SEQ ID No. 3) on the No. 4 chromosome in the genome of the duck to be detected is a heterozygote of A and G, the genotype of the duck to be detected is A: G type;
(B2) determining the color property of the duck eggshell to be detected as follows: if the genotype of the duck to be detected is G: G, the color of the eggshell of the duck to be detected is (or is candidate to be) white; and if the genotype of the duck to be detected is A: A or A: G, the color of the eggshell of the duck to be detected is (or is candidate to be) cyan.
Further, in the step (B1), whether the 47418074 th deoxyribonucleotide (i.e., the 507 th deoxyribonucleotide of SEQ ID No. 3) on chromosome 4 in the genome of the duck to be tested is a, G, a and G can be detected according to a method comprising the following steps: performing PCR amplification by using the genomic DNA of the duck to be detected as a template and adopting the primer pair to obtain an amplification product; and then sequencing the amplification product, and determining whether the 47418074 th deoxyribonucleotide on the chromosome 4 (namely the 507 th deoxyribonucleotide of SEQ ID No. 3) in the genome of the duck to be detected is A, G or A and G according to a sequencing result.
In a third aspect, the invention claims any of the following methods:
the method I comprises the following steps: a method for breeding a duck individual homozygous for green-shell egg production can comprise the following steps:
(C1) detecting the genotype of the duck to be detected according to the step (B1);
(C2) according to the detection result, selecting an individual with genotype A: A, namely (or candidate) a duck individual homozygous for laying green-shell eggs.
Method II: a method for breeding a duck individual homozygous for white-shell egg production can comprise the following steps:
(D1) detecting the genotype of the duck to be detected according to the step (B1);
(D2) and selecting an individual with the genotype G: G according to the detection result, namely (or selecting as a candidate) a duck individual homozygous for egg white.
Method III: a method for improving the green shell rate of a duck group comprises the following steps:
(E1) detecting the genotype of each individual in the duck population according to the previous step (B1);
(E2) according to the detection result, individuals with genotypes A: A are selected from the duck population to be reserved, and individuals with genotypes G: G and A: G are removed, so that the aim of purifying the green shell population can be achieved through first-generation breeding.
Method IV: a method for improving the white shell rate of a duck group comprises the following steps:
(F1) detecting the genotype of each individual in the duck population according to the previous step (B1);
(F2) according to the detection result, selecting individuals with the genotype G: G from the duck population, reserving the individuals, and rejecting the individuals with the genotypes A: A and A: G, so that the aim of purifying the white shell population can be achieved through first-generation breeding.
In a fourth aspect, the invention claims a substance having at least one of the following functions (a) - (E), which is any one of the substances described above for detecting whether the 47418074 th deoxyribonucleotide on chromosome 4 (i.e. the 507 th deoxyribonucleotide of SEQ ID No. 3) in the duck genome is a, G, or a and G (i.e. the primer pair described above in the first aspect or the reagent or kit containing the primer pair);
(A) detecting the color character of the duck eggshell to be detected;
(B) breeding duck individuals homozygous for green-shell eggs;
(C) breeding duck individuals homozygous for white-shell eggs;
(D) the green shell rate of the duck group is improved;
(E) the white shell rate of the duck group is improved.
In a fifth aspect, the invention claims molecular markers.
The molecular marker claimed by the invention takes duck genome DNA as a template, and adopts the primer pair in the first aspect to perform PCR amplification to obtain an amplification product;
further, the molecular marker is molecular marker 1 and/or molecular marker 2;
the molecular marker 1 (corresponding to the situation that the 47418074 th deoxyribonucleotide on the No. 4 chromosome in the duck genome is A) can be any one of the following:
(b1) a DNA molecule shown in SEQ ID No.3, wherein the 507 th deoxyribonucleotide of the SEQ ID No.3 is A;
(b2) DNA molecules which are derived from ducks, have more than 98% of similarity with the sequence defined in (b1), and are unchanged corresponding to the 47418074 th deoxyribonucleotide on the chromosome 4 in the duck genome;
(b3) a DNA molecule derived from a duck and having 5 or less different nucleotides from the sequence defined in (b 1); the 5 or less different nucleotides do not comprise 47418074 th deoxyribonucleotide on chromosome 4 in a duck genome;
the molecular marker 2 (corresponding to the situation that the 47418074 th deoxyribonucleotide on the No. 4 chromosome in the duck genome is G) can be any one of the following:
(c1) a DNA molecule shown in SEQ ID No.3, wherein the 507 th deoxyribonucleotide of the SEQ ID No.3 is G;
(c2) DNA molecules which are derived from ducks, have more than 98% of similarity with the sequence defined in (c1) and are unchanged corresponding to the 47418074 th deoxyribonucleotide on the chromosome 4 in the duck genome;
(c3) a DNA molecule derived from a duck and having 5 or less different nucleotides from the sequence defined in (c 1); the 5 or less different nucleotides do not comprise 47418074 th deoxyribonucleotide on chromosome 4 in the duck genome (namely 507 th deoxyribonucleotide of SEQ ID No. 3).
The 507 th position of SEQ ID No.3 corresponds to the 47418074 th deoxyribonucleotide on the 4 th chromosome in the duck genome.
In a sixth aspect, the invention claims any of the following methods:
the method I': a method for breeding a duck individual homozygous for green-shell egg production can comprise the following steps:
(C1') detecting the molecular marker contained in the genomic DNA of the duck to be detected;
(C2') according to the detection result, selecting the individual only containing the molecular marker 1, namely (or selecting the individual as) the duck individual homozygous for laying green-shell eggs.
Method II': a method for breeding a duck individual homozygous for white-shell egg production can comprise the following steps:
(D1') detecting the foreign molecular marker contained in the genomic DNA of the duck to be detected;
(D2') according to the detection result, selecting the individual only containing the molecular marker 2, namely, the individual is or is selected as the duck individual which is homozygous for egg white.
Method III': a method for improving the green shell rate of a duck group comprises the following steps:
(E1') detecting the type of molecular marker contained in each individual of the duck population;
(E2') according to the detection result, selecting and reserving the individual containing the molecular marker 1 from the duck population, and rejecting other individuals, thereby ensuring that the aim of purifying the green-shell population can be achieved through first-generation breeding.
Method IV': a method for improving the white shell rate of a duck group comprises the following steps:
(F1') detecting the type of molecular marker contained in each individual of the duck population;
(F2') according to the detection result, selecting and reserving the individual only containing the molecular marker 2 from the duck population, and rejecting other individuals, thereby ensuring that the aim of purifying the white shell population can be achieved through first-generation breeding.
In a seventh aspect, the invention claims any of the following applications:
(G1) use of a substance as described in the fourth aspect or a molecular marker as described in the fifth aspect for, or for the preparation of a product for, breeding of a duck individual homozygous for green shell egg laying;
(G2) use of a substance as described in the fourth aspect or a molecular marker as described in the fifth aspect for, or for the preparation of a product for, breeding of a duck individual homozygous for white-shell egg production;
(G3) use of a substance as described in the fourth aspect or a molecular marker as described in the fifth aspect for increasing the rate of blue shells in a duck population, or for the manufacture of a product for increasing the rate of blue shells in a duck population;
(G4) use of a substance as described in the fourth aspect or a molecular marker as described in the fifth aspect for increasing the white shell rate of a duck population, or for the manufacture of a product for increasing the white shell rate of a duck population;
(G5) the application of the molecular marker in the fifth aspect in identifying the color character of the duck eggshell to be detected or preparing a product for identifying the color character of the duck eggshell to be detected;
(G6) use of a substance as described in the fourth aspect or a molecular marker as described in the fifth aspect or a method as described in the second, third or sixth aspect in duck breeding.
In the invention, the 47418074 th deoxyribonucleotide on chromosome 4 in the Duck Genome takes BGI _1.0Duck Genome (http:// www.duckbase.org/Download) as a reference sequence.
In the present invention, the duck may be any duck variety. In a particular embodiment of the invention, the duck is in particular a Beijing duck.
The inventor of the invention discovers SNP (single nucleotide polymorphism) related to the color of an eggshell by whole genome sequencing of a large number of duck samples and discovers that a single nucleotide base mutation of G > A exists at 47418074bp of chromosome 4 of a duck genome through whole genome association (GWAS) analysis, and the mutation affects the green/white character of the color of the eggshell of the duck. Based on the genotype of the SNP locus, the green shell/white shell character of the duck can be screened, the genotype of the green shell character is AA type and AG type, and the genotype of the white shell character is GG type.
The invention has the beneficial effects that:
1. can realize rapid purification of eggshell color and can reach a purification state only through first generation breeding.
2. The early selection of the color character of the eggshell can be realized.
The method can save production cost and accelerate genetic progress, better serves duck breeding, and has great economic application value and scientific research value.
Drawings
FIG. 1 is a graph showing the results of the association analysis of all genes in example 1, in which: the abscissa represents the chromosome number of the duck; the ordinate represents the-logP value.
Fig. 2 shows chr 4: 47418074 partial sequencing results of an exemplary sample. A is a sample of AG genotype; b is a sample of AA genotype; c is a sample of GG genotype.
Detailed Description
The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
Example 1 determination of correlation between SNP molecular markers and Eggshell color traits
Experimental materials: beijing duck M1 group female ducks (400 in total) bred in Changping breeding base of China academy of agricultural sciences, Beijing animal husbandry and veterinary institute.
First, measuring color character of egg shell
The method comprises the steps of feeding female ducks of an M1 population to an egg laying period, transferring each female duck to an individual egg laying cage for feeding before laying, continuously recording the eggshell color of duck eggs in each cage for 5 days in the egg laying period, and finally determining the eggshell color character of each female duck.
Second, genome sequencing
1. Collecting a blood sample: collecting duck wing venous blood to be detected by using a heparin sodium anticoagulant blood collection tube, and storing at-20 ℃ for later use.
2. Whole blood genomic DNA extraction: genomic DNA of the whole blood sample was extracted, and the procedure was a method conventional in the art.
3. Genotyping: taking the genome DNA of each duck to be detected, and carrying out whole genome individual re-sequencing by adopting a Hiseq X-Ten sequencing platform of Illumina, wherein the sequencing depth of each individual is about 5X, and the specific method refers to a standard operation flow provided by Illumina. After the off-line data is subjected to quality control, two bioinformatics software of BWA and GATK are adopted for distribution to carry out sequence comparison and genotype extraction.
Third, whole genome association analysis of eggshell color traits
And performing whole genome correlation analysis on the color traits and genotypes of the eggshells by adopting a compressed mixed linear model of EMMAX software for statistical analysis. The results show that chr 4: the nucleotide at position 47418074 (corresponding to deoxyribonucleotide 507 of SEQ ID No. 3) mutation (G > a) was significantly associated with the eggshell color trait, as shown in fig. 1. Fig. 2 shows chr 4: 47418074 (i.e., position 507 of SEQ ID No. 3) partial sequencing results of an exemplary sample. The eggshell color phenotype is white when the genotype of the locus is G: G, and the eggshell color phenotype is cyan when the genotype is A: G or A: A.
Example 2, for the identification of chr 4: primer design for nucleotide polymorphism at position 47418074
Based on the whole genome sequencing results of example 1, designed to identify chr 4: 47418074, the following primers:
an upstream primer: 5'-CGAGGCAGGGGACCTATCTT-3' (SEQ ID No. 1);
a downstream primer: 5'-CTGTGCAGACTAAGAGGCGT-3' (SEQ ID No. 2).
Aiming at the gene group of the duck, chr 4: 47418074 (i.e., 507 th deoxyribonucleotide of SEQ ID No. 3) is A homozygote (marked as A: A genotype), and the theoretical amplification product has only one nucleotide, i.e., 507 th deoxyribonucleotide is DNA fragment shown in SEQ ID No.3 of A.
Aiming at the gene group of the duck, chr 4: 47418074 (i.e., 507-position deoxyribonucleotide of SEQ ID No. 3) is G homozygote (marked as G: G genotype), and theoretical amplification product has only one, i.e., 507-position deoxyribonucleotide is G DNA fragment shown in SEQ ID No. 3.
Aiming at the gene group of the duck, chr 4: 47418074 (i.e., 507-position deoxyribonucleotide of SEQ ID No. 3) is a heterozygote of A and G (marked as A: G genotype), theoretical amplification products have two DNA fragments, namely, one DNA fragment shown by SEQ ID No.3 with 507-position deoxyribonucleotide being A and the other DNA fragment shown by SEQ ID No.3 with 507-position deoxyribonucleotide being G.
Example 3 isolation of Peking Duck population M1 according to genotype M1 white shell group and M1 Green shell group
Experimental materials: m1 population of Beijing ducks (total 600 ducks) bred in Changping breeding base of the institute of livestock and veterinary, Beijing institute of agricultural sciences, China.
Obtaining the genotype of each individual
1. Collecting a blood sample: collecting all duck wing venous blood of M1 population to be detected by heparin sodium anticoagulation blood collection tube, and storing at-20 ℃ for later use.
2. Whole blood genomic DNA extraction: genomic DNA of the whole blood sample was extracted, and the procedure was a method conventional in the art.
3. And (3) genotype judgment: taking the genome DNA of each duck to be detected as a template, carrying out PCR amplification by using the primers designed in the embodiment 2, sequencing the obtained product by a Sanger method to obtain a molecular sequence, and judging the genotype of each individual.
Establishing M1 white shell population and M1 green shell population
1. Establishing M1 white shell population: individuals with genotype judged as G: G were kept to form white-shell populations (125 ducks total; male-female ratio 1: 4).
2. Establishing M1 green shell population: individuals with genotypes judged as A: A were kept to form a green shell population (125 ducks total; male-female ratio 1: 4).
3. And (3) verifying the genetic stability of the character: in order to ensure that the two groups established in the test can be stably inherited, the test also detects the genotypes and phenotypes of the filial generations of the two groups, and the result shows that the character can be stably inherited without separation.
Sequence listing
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taacagcagg ctgttctcac cttcattggg cgaaggggtg cagcaggttg ggtgaactgt 180
gagcagggct cattctttgt ccccatgcac acttacatga cacttctttg tgtccagttg 240
atgttagtac aacatggaag cttctgagtc aggcttgggg cagggttagc tttgacagac 300
acgtgtcatg cagtatccct gattcctaca ggcagtttgc caacttcaca cccttcaact 360
atcttaaaca ggctgaaatg caggttgtca agctaggaag gttttgtttg tttgtttggt 420
ttgttttgtt ttgtttttcc atattctcaa atgttggctt ggataagaaa tgaccagatg 480
tgttcttgcc agcaatttta tgtaacrttg ttttcttttt ctagtccacc gttctccggg 540
gctgatcaaa tggtgacata taatttgatt ctcaaaggga ttgaaaaact ggactttcct 600
aaaataataa caaggcggcc tgaggatttg atccgcagac tttgcaggta ggaggggcag 660
tttgtgacgg agactgggtg tagatagttt gagctattta agatccagac taaagtcata 720
aattccttac aactccattt aacattgtta tgcctaacat ggtccattct gattatgttc 780
agtacacatg catgcctctg agcatgtcac gcctcttagt ctgcacag 828
Claims (10)
1. The application of the substance for detecting whether the 507 th deoxyribonucleotide of SEQ ID No.3 in a duck genome is G type, A type or A type in any one of the following types:
(A1) identifying the color character of the duck egg shell to be detected;
(A2) preparing a product for identifying the color character of the duck eggshell to be detected.
2. Use according to claim 1, characterized in that: the substance is a primer pair or a reagent or a kit containing the primer pair;
the primer pair consists of an upstream primer and a downstream primer; the upstream primer is designed according to the upstream sequence of the 507 th deoxyribonucleotide of SEQ ID No.3 in the duck genome; the downstream primer is designed according to the downstream sequence of the 507 th deoxyribonucleotide of SEQ ID No.3 in the duck genome.
3. Use according to claim 2, characterized in that:
the upstream primer is as follows (a 1):
(a1) DNA molecule shown in SEQ ID No. 1;
the downstream primer is as follows (a 3):
(a3) DNA molecule shown in SEQ ID No. 2.
4. A method for identifying the color character of a duck eggshell to be detected comprises the following steps:
(B1) detecting whether the 507 th deoxyribonucleotide gene of SEQ ID No.3 in the genome of the duck to be detected is G type, A type or A type G type;
(B2) determining the color property of the duck eggshell to be detected as follows: if the genotype of the duck to be detected is G, the color of the eggshell of the duck to be detected is white or is a candidate; and if the genotype of the duck to be detected is A: A or A: G, the color of the eggshell of the duck to be detected is cyan or is a candidate.
5. The method of claim 4, wherein: in the step (B1), the genotype of the 507 th deoxyribonucleotide of SEQ ID No.3 in the genome of the duck to be detected is G: G, A: A or A: G according to the method comprising the following steps: carrying out PCR amplification by using the genomic DNA of the duck to be detected as a template and the primer pair in claim 2 or 3 to obtain an amplification product; and sequencing the amplification product, and determining that the genotype of the 507 th deoxyribonucleotide of SEQ ID No.3 in the genome of the duck to be detected is G: G type, A: A type or A: G type according to a sequencing result.
6. Any one of the following methods:
the method I comprises the following steps: a method for breeding a duck individual homozygous for green-shell egg production comprises the following steps:
(C1) detecting the genotype of the duck to be tested according to the step (B1) of claim 4 or 5;
(C2) selecting individuals with genotype A: A according to the detection result, namely or selecting the individuals as the duck individuals homozygous for laying green-shell eggs;
method II: a method for breeding a duck individual homozygous for white-shell egg production comprises the following steps:
(D1) detecting the genotype of the duck to be tested according to the step (B1) of claim 4 or 5;
(D2) selecting an individual with G, G type genotype according to the detection result, namely, the individual is or is selected as a duck individual homozygous for egg white;
method III: a method for improving the green shell rate of a duck group comprises the following steps:
(E1) detecting the genotype of each individual in the duck population according to step (B1) of claim 4 or 5;
(E2) selecting individuals with genotype A: A from the duck population according to the detection result, reserving the individuals, and rejecting the individuals with genotypes G: G and A: G;
method IV: a method for improving the white shell rate of a duck group comprises the following steps:
(F1) detecting the genotype of each individual in the duck population according to step (B1) of claim 4 or 5;
(F2) and selecting and reserving the individuals with the genotypes G to G from the duck population according to the detection result, and rejecting the individuals with the genotypes A to A and A to G.
7. A substance having at least one function selected from the following (A) to (E), characterized in that: the substance is the substance which is used for detecting the G: G type, A: A type or A: G type of the 507 th deoxyribonucleotide of SEQ ID No.3 in a duck genome as claimed in any one of claims 1 to 3;
(A) detecting the color character of the duck eggshell to be detected;
(B) breeding duck individuals homozygous for green-shell eggs;
(C) breeding duck individuals homozygous for white-shell eggs;
(D) the green shell rate of the duck group is improved;
(E) the white shell rate of the duck group is improved.
8. A molecular marker characterized by: the molecular marker is a molecular marker 1 and/or a molecular marker 2;
the molecular marker 1 is:
(b1) a DNA molecule shown in SEQ ID No.3, wherein the 507 th deoxyribonucleotide of the SEQ ID No.3 is A;
the molecular marker 2 is:
(c1) the DNA molecule shown in SEQ ID No.3, wherein the 507 th deoxyribonucleotide of the SEQ ID No.3 is G.
9. Any one of the following methods:
the method I': a method for breeding a duck individual homozygous for green-shell egg production comprises the following steps:
(C1') detecting which molecular marker in the claim 8 is contained in the genome DNA of the duck to be detected;
(C2') according to the detection result, selecting an individual containing only the molecular marker 1 of claim 8, namely, the individual is or is selected as a duck individual homozygous for laying green-shell eggs;
method II': a method for breeding a duck individual homozygous for white-shell egg production comprises the following steps:
(D1') detecting which molecular marker in the claim 8 is contained in the genome DNA of the duck to be detected;
(D2') selecting, according to the results of the detection, an individual containing only the molecular marker 2 of claim 8, that is, or is selected as an individual homozygous for a duck producing white-shell egg;
method III': a method for improving the green shell rate of a duck group comprises the following steps:
(E1') determining which molecular marker of claim 8 each individual in a duck population contains;
(E2') selecting and reserving an individual containing only the molecular marker 1 of claim 8 from the duck population according to the detection result, and rejecting other individuals;
method IV': a method for improving the white shell rate of a duck group comprises the following steps:
(F1') determining for each individual in a duck population which molecular marker of claim 8 is contained;
(F2') selecting and reserving an individual containing only the molecular marker 2 of claim 8 from the duck population according to the detection result, and rejecting other individuals.
10. Any of the following applications:
(G1) use of the substance of claim 7 or the molecular marker of claim 8 for breeding a duck individual homozygous for laying green-shell eggs, or for preparing a product for breeding a duck individual homozygous for laying green-shell eggs;
(G2) use of the substance of claim 7 or the molecular marker of claim 8 for breeding, or for preparing a product for breeding, a duck individual homozygous for white-shell egg production;
(G3) use of the substance of claim 7 or the molecular marker of claim 8 for increasing the rate of blue shells in a duck population, or for preparing a product for increasing the rate of blue shells in a duck population;
(G4) use of the substance of claim 7 or the molecular marker of claim 8 for increasing the white shell rate of a duck population, or for the preparation of a product for increasing the white shell rate of a duck population;
(G5) use of the molecular marker of claim 8 for identifying a color trait of duck egg shells to be tested, or for preparing a product for identifying a color trait of duck egg shells to be tested;
(G6) use of the substance of claim 7 or the molecular marker of claim 8 or the method of any one of claims 4-6 and 9 in duck breeding.
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| CN114438224A (en) * | 2022-01-27 | 2022-05-06 | 江苏省家禽科学研究所 | Molecular marker and primer of duck eggshell character related genotype, and detection method and application thereof |
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| CN108165639A (en) * | 2018-03-12 | 2018-06-15 | 中国农业科学院北京畜牧兽医研究所 | A kind of and the relevant molecular labeling of fleshy duck fodder transformation efficiency character and its application |
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| CN108165639A (en) * | 2018-03-12 | 2018-06-15 | 中国农业科学院北京畜牧兽医研究所 | A kind of and the relevant molecular labeling of fleshy duck fodder transformation efficiency character and its application |
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