CN110679795A - Bullfrog feed additive and preparation method and application thereof - Google Patents
Bullfrog feed additive and preparation method and application thereof Download PDFInfo
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- CN110679795A CN110679795A CN201911127897.3A CN201911127897A CN110679795A CN 110679795 A CN110679795 A CN 110679795A CN 201911127897 A CN201911127897 A CN 201911127897A CN 110679795 A CN110679795 A CN 110679795A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a bullfrog feed additive and a preparation method and application thereof, wherein the bullfrog feed additive comprises the following components in parts by weight: 20-40 parts of degreased gardenia dregs, 20-30 parts of microcos paniculata, 15-24 parts of dendrobium officinale, 5-10 parts of wormwood, 5-10 parts of dandelion, 10-20 parts of germinated brown rice, 5-10 parts of compound vitamin and 5-10 parts of salt. The invention adopts degreased gardenia dregs, microcos paniculata, dendrobium officinale, wormwood, dandelion, germinated brown rice, multivitamin and salt as raw materials to prepare the bullfrog feed additive, the formula is easy to obtain, the additive has no toxic action and is cheap, the preparation method of the additive is simple and complex, the operability is strong, the preparation cost is low, and the additive is suitable for wide popularization and use.
Description
Technical Field
The invention relates to the technical field of bullfrog breeding, in particular to a bullfrog feed additive and a preparation method and application thereof.
Background
Bullfrog (Rana catesbeiana Shaw) belongs to the vertebrate subgenus, Amphina, Annelida, Ranidae. The original place of the bullfrog is a swamp area in the mountain range of los angeles in north america, and the bullfrog is a large edible frog, which is second to the forest frog in frogs. The buzz sounds like a bullhorn, so the name of Bullfrog is obtained. The food has rich meat quality, is delicious and tasty, is a wild food with high protein, low fat and low cholesterol, has good commodity, and is introduced into Canada, Mexico, south America countries, Japan and the like for cultivation in sequence. Bullfrog is introduced into China in 1959 for domestication, and is bred in the middle and south of China in 1986 in large quantities.
In recent years, the breeding industry of bullfrogs is rapidly developed in southeast provinces of China, and under the intensive breeding condition, because of large amount of compound feed ingestion and lack of movement, the bullfrogs generally have high body fat, so that the quality and the edible proportion of the bullfrogs are reduced, and the breeding cost is improved; and the incidence of various bullfrog diseases is increased sharply due to the continuous improvement of breeding density, the pollution of breeding environment and other reasons, and antibiotics and disinfectants are mainly used for preventing and treating the bullfrog diseases in production in order to prevent the bullfrog diseases from happening, so the serious consequences are caused by long-term use of the antibiotics and disinfectants: the abuse of antibiotics causes the drug resistance of pathogenic bacteria to be enhanced, so that the bullfrog disease is more difficult to treat; the disinfectant has great toxic and side effects, strong local stimulation and carcinogenesis, and causes severe stress of bullfrogs; the aquaculture water body is destroyed, the water environment is polluted, and the immunity of the fish body is reduced; the medicine residue is increased, the aquatic product is safe and worried, the quality is reduced, and the human health is harmed.
Therefore, there is a need to provide a natural, non-hazardous, nutritional and healthy additive that can improve the eating quality of bullfrogs.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: overcomes the defects of the prior art, and provides the bullfrog feed additive which can reduce the fat content of the bullfrog and improve the eating quality of the bullfrog muscle, the preparation method and the application thereof.
The technical scheme adopted by the invention for solving the technical problems is as follows: the bullfrog feed additive comprises the following components in parts by weight: 20-40 parts of degreased gardenia dregs, 20-30 parts of microcos paniculata, 15-24 parts of dendrobium officinale, 5-10 parts of wormwood, 5-10 parts of dandelion, 10-20 parts of germinated brown rice, 5-10 parts of compound vitamin and 5-10 parts of salt.
Preferably, the degreased gardenia dregs are 25-35 parts, the microcos paniculata is 22-28 parts, the dendrobium officinale is 18-20 parts, the wormwood is 8-10 parts, the dandelion is 8-10 parts, the germinated brown rice is 12-16 parts, the vitamin complex is 6-8 parts, and the salt is 5-8 parts.
Preferably, the degreased gardenia dregs are 30 parts, the microcos paniculata is 25 parts, the dendrobium officinale is 20 parts, the wormwood is 9 parts, the dandelion is 9 parts, the germinated brown rice is 15 parts, the vitamin complex is 8 parts and the salt is 6 parts.
Further, the compound vitamin comprises vitamin A, vitamin C and vitamin E, and the proportion of the components is 1: 1: 1.
the invention also provides a preparation method of the bullfrog feed additive, which comprises the following steps:
1) pretreating the cloth residue leaves and the dendrobium officinale: selecting fresh cloth residue leaves and dendrobium officinale, cutting the cloth residue leaves and the dendrobium officinale into small pieces, putting the small pieces into a pulping machine, adding water with the weight 5-10 times that of the cloth residue leaves for pulping, and filtering to obtain mixed juice for later use;
2) preparing the compound vitamin: the components in proportion are 1: 1: 1, weighing vitamin A, vitamin C and vitamin E, crushing and uniformly mixing to obtain compound vitamin for later use;
3) pretreatment of raw materials: crushing defatted gardenia dregs, wormwood, dandelion, germinated brown rice and compound vitamin to obtain premix for later use;
4) uniformly mixing the mixed juice prepared in the step 1), the compound vitamin prepared in the step 2), the premix prepared in the step 3) and the salt, and drying to obtain the additive.
Further, the preparation steps of the degreased gardenia dregs are as follows: selecting fresh gardenia fruits, shelling, and adding the fresh gardenia fruits into the shelled kernels according to the material-liquid ratio of 1: extracting with butane 20-50, and drying to obtain defatted fructus Gardeniae cake.
Further, the particle size after the pulverization in the step 2) and the step 3) is 100-150 meshes.
The invention also provides an application of the bullfrog feed additive, which is characterized in that: the additive is used in the preparation process of bullfrog feed.
Further, the preparation process of the bullfrog feed comprises the following steps: mixing and stirring evenly fish meal, bean pulp, corn flour, wheat bran, seaweed meal, rice bran, river snail powder and bullfrog feed additive, crushing and sieving with a sieve of 100 meshes and 120 meshes to obtain the bullfrog feed.
Further, 10-20 parts of fish meal, 30-40 parts of soybean meal, 5-10 parts of corn flour, 10-15 parts of wheat bran, 10-15 parts of seaweed meal, 5-10 parts of rice bran, 5-10 parts of viviparidae powder and 20-25 parts of bullfrog feed additive.
The bullfrog feed additive and the preparation method and the application thereof have the beneficial effects that:
(1) the invention adopts degreased gardenia dregs, microcos paniculata, dendrobium officinale, wormwood, dandelion, germinated brown rice, compound vitamin and salt as raw materials to prepare the bullfrog feed additive, the formula is easy to obtain, the additive has no toxic action and is cheap, the preparation method of the additive is simple and complex, the operability is strong, the preparation cost is low, and the additive is suitable for wide popularization and use;
(2) the additive can enhance the oxidation resistance of bullfrog, eliminate the damage of free radicals and active oxygen to the bullfrog in the lipid peroxidation process and improve the immunity of the bullfrog by adding the compound vitamin; the germinated brown rice contains various vitamins, microelements, amino acids and other nutritional ingredients and protease, antioxidant enzyme and other active substances, and can improve the nutritional value of the additive and improve the oxidation resistance of bullfrog.
(3) By adding the degreased gardenia dregs into the additive, the intestinal protease and lipase activity of the bullfrogs can be improved, the digestion and absorption of feed nutrition are promoted, the content of saturated fatty acid in bullfrog muscles is effectively reduced, the digestion and absorption rate of the bullfrogs on the feed and the muscle quality of the bullfrogs are improved, and the weight gain rate and the feed efficiency of the bullfrogs are improved;
(4) the additive of the invention can reduce the activity of peroxidase by adding the microcos paniculata, reduce the damage of peroxide to the liver of bullfrog, maintain the normal physiological metabolic process, improve the disease resistance of bullfrog, and keep the freshness and good color of the feed.
Detailed Description
The invention is further illustrated by the following examples, which are not intended to limit the scope of the invention in any way.
Example 1
A bullfrog feed additive and a preparation method thereof comprise the following steps:
1) pretreating the cloth residue leaves and the dendrobium officinale: selecting fresh cloth residue leaves and dendrobium officinale, cutting 25 parts of the cloth residue leaves and 20 parts of the dendrobium officinale into small pieces, putting the small pieces into a pulping machine, adding 5 times of water by weight for pulping, and filtering to obtain mixed juice for later use;
2) preparing the compound vitamin: the components in proportion are 1: 1: 1, weighing vitamin A, vitamin C and vitamin E, then crushing to 150 meshes, and uniformly mixing to obtain compound vitamin for later use;
3) preparation of degreased gardenia dregs: selecting fresh gardenia fruits, shelling, and adding the fresh gardenia fruits into the shelled kernels according to the material-liquid ratio of 1: 30, adding butane to extract and drying to obtain degreased gardenia dregs.
4) Pretreatment of raw materials: crushing 30 parts of degreased gardenia dregs, 9 parts of wormwood, 9 parts of dandelion, 15 parts of germinated brown rice and 8 parts of compound vitamin to 150 meshes to obtain premix for later use;
5) uniformly mixing the mixed juice prepared in the step 1), the compound vitamin prepared in the step 2), the premix prepared in the step 4) and 6 parts of salt, and drying to obtain the additive.
Example 2
A bullfrog feed additive and a preparation method thereof comprise the following steps:
1) pretreating the cloth residue leaves and the dendrobium officinale: selecting fresh cloth residue leaves and dendrobium officinale, cutting 20 parts of the cloth residue leaves and 15 parts of the dendrobium officinale into small pieces, putting the small pieces into a pulping machine, adding 5 times of water by weight for pulping, and filtering to obtain mixed juice for later use;
2) preparing the compound vitamin: the components in proportion are 1: 1: 1, weighing vitamin A, vitamin C and vitamin E, then crushing to 150 meshes, and uniformly mixing to obtain compound vitamin for later use;
3) preparation of degreased gardenia dregs: selecting fresh gardenia fruits, shelling, and adding the fresh gardenia fruits into the shelled kernels according to the material-liquid ratio of 1: 30, adding butane to extract and drying to obtain degreased gardenia dregs.
4) Pretreatment of raw materials: crushing 20 parts of degreased gardenia dregs, 5 parts of wormwood, 5 parts of dandelion, 10 parts of germinated brown rice and 5 parts of compound vitamin to 150 meshes to obtain premix for later use;
5) uniformly mixing the mixed juice prepared in the step 1), the compound vitamin prepared in the step 2), the premix prepared in the step 4) and 5 parts of salt, and drying to obtain the additive.
Example 3
A bullfrog feed additive and a preparation method thereof comprise the following steps:
1) pretreating the cloth residue leaves and the dendrobium officinale: selecting fresh cloth residue leaves and dendrobium officinale, cutting 30 parts of the cloth residue leaves and 24 parts of the dendrobium officinale into small pieces, putting the small pieces into a pulping machine, adding 5 times of water by weight for pulping, and filtering to obtain mixed juice for later use;
2) preparing the compound vitamin: the components in proportion are 1: 1: 1, weighing vitamin A, vitamin C and vitamin E, then crushing to 150 meshes, and uniformly mixing to obtain compound vitamin for later use;
3) preparation of degreased gardenia dregs: selecting fresh gardenia fruits, shelling, and adding the fresh gardenia fruits into the shelled kernels according to the material-liquid ratio of 1: 30, adding butane to extract and drying to obtain degreased gardenia dregs.
4) Pretreatment of raw materials: crushing 40 parts of degreased gardenia dregs, 10 parts of wormwood, 10 parts of dandelion, 20 parts of germinated brown rice and 10 parts of compound vitamin to 150 meshes to obtain premix for later use;
5) uniformly mixing the mixed juice prepared in the step 1), the compound vitamin prepared in the step 2), the premix prepared in the step 4) and 10 parts of salt, and drying to obtain the additive.
Comparative example 1
This comparative example differs from example 1 in that: defatted fructus Gardeniae cake is not added.
Comparative example 2
This comparative example differs from example 1 in that: the folium Microcoris Paniculatae is not added.
Comparative example 3
This comparative example differs from example 1 in that: defatted fructus Gardeniae cake and folium Microcoris Paniculatae are not added.
Selecting 60 bullfrogs in a large bullfrog farm, randomly distributing into 6 groups, ensuring that the weight, the size and the health condition are basically consistent, respectively adopting the methods of examples 1-3 and comparative examples 1-3 to prepare the bullfrog feed additive, uniformly mixing 20 parts of the bullfrog feed additive with 20 parts of fish meal, 40 parts of bean pulp, 10 parts of corn meal, 15 parts of wheat bran, 15 parts of seaweed meal, 10 parts of rice bran and 10 parts of viviparidae powder, and crushing to obtain the bullfrog feed for feeding the bullfrogs in the test groups, wherein the corresponding composition ratio of the bullfrog feed additive is shown in table 1, and after 8 weeks of cultivation, collecting the bullfrog feces once every other day until enough amount of the feces is collected. And (3) determining the digestibility of the bullfrog to the protein and the lipid in the feed, wherein the protein is determined by adopting a Kjeldahl method, and the lipid is determined by adopting a Soxhlet extraction method. The method for calculating the digestibility of the protein comprises the following steps: (protein content in feed-protein content in feces)/protein content in feed, the method for calculating the digestibility of lipid is as follows: (lipid content in feed-lipid content in feces)/protein content in feed.
TABLE 1 composition Table of Bullfrog feed additives
TABLE 2 protein and lipid digestibility
As can be seen from table 2, the feed additive of the present invention can improve the digestibility of protein and lipid, and the digestibility of protein and lipid in example 1 is significantly higher than that in comparative example 1, indicating that the defatted gardenia seed meal can improve the digestibility of protein and lipid by bullfrog.
After 8 weeks of culture, all treatment groups sample bullfrog and dissect the bullfrog, weigh the liver and internal organs, quickly take out the liver and intestinal tract, and store in a refrigerator at-80 ℃ for testing. Liver superoxide dismutase, peroxidase, intestinal lipase and intestinal protease are all determined by adopting Nanjing built kit.
TABLE 3 enzyme Activity measurement Table
As can be seen from the above table, the bullfrog feed additive of the invention can reduce the activity of peroxidase and improve the oxidation resistance of bullfrogs; the additive can increase activity of intestinal lipase and protease, promote digestion and absorption of protein and lipid, and improve eating quality of bullfrog muscle.
Although the present invention has been described with reference to a preferred embodiment, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (10)
1. A bullfrog feed additive is characterized in that: the paint comprises the following components in parts by weight: 20-40 parts of degreased gardenia dregs, 20-30 parts of microcos paniculata, 15-24 parts of dendrobium officinale, 5-10 parts of wormwood, 5-10 parts of dandelion, 10-20 parts of germinated brown rice, 5-10 parts of compound vitamin and 5-10 parts of salt.
2. The bullfrog feed additive of claim 1 wherein: 25-35 parts of degreased gardenia dregs, 22-28 parts of microcos paniculata, 18-20 parts of dendrobium officinale, 8-10 parts of wormwood, 8-10 parts of dandelion, 12-16 parts of germinated brown rice, 6-8 parts of vitamin complex and 5-8 parts of salt.
3. The bullfrog feed additive of claim 2 wherein: 30 parts of degreased gardenia dregs, 25 parts of microcos paniculata, 20 parts of dendrobium officinale, 9 parts of wormwood, 9 parts of dandelion, 15 parts of germinated brown rice, 8 parts of compound vitamin and 6 parts of salt.
4. The bullfrog feed additive of claim 1 wherein: the compound vitamin comprises vitamin A, vitamin C and vitamin E, and the proportion of the components is 1: 1: 1.
5. a method for preparing a bullfrog feed additive as claimed in claim 1, wherein: the method comprises the following steps:
1) pretreating the cloth residue leaves and the dendrobium officinale: selecting fresh cloth residue leaves and dendrobium officinale, cutting the cloth residue leaves and the dendrobium officinale into small pieces, putting the small pieces into a pulping machine, adding water with the weight 5-10 times that of the cloth residue leaves for pulping, and filtering to obtain mixed juice for later use;
2) preparing the compound vitamin: the components in proportion are 1: 1: 1, weighing vitamin A, vitamin C and vitamin E, crushing and uniformly mixing to obtain compound vitamin for later use;
3) pretreatment of raw materials: crushing defatted gardenia dregs, wormwood, dandelion, germinated brown rice and compound vitamin to obtain premix for later use;
4) uniformly mixing the mixed juice prepared in the step 1), the compound vitamin prepared in the step 2), the premix prepared in the step 3) and the salt, and drying to obtain the additive.
6. The method of claim 5, wherein the method comprises the steps of: the preparation method of the degreased gardenia dregs comprises the following steps: selecting fresh gardenia fruits, shelling, and adding the fresh gardenia fruits into the shelled kernels according to the material-liquid ratio of 1: extracting with butane 20-50, and drying to obtain defatted fructus Gardeniae cake.
7. The method of claim 5, wherein the method comprises the steps of: the particle size after the pulverization in the step 2) and the step 3) is 100-150 meshes.
8. Use of a bullfrog feed additive according to claim 1 wherein: the additive is used in the preparation process of bullfrog feed.
9. The use of a bullfrog feed additive as claimed in claim 8, wherein: the preparation process of the bullfrog feed comprises the following steps: mixing and stirring evenly fish meal, bean pulp, corn flour, wheat bran, seaweed meal, rice bran, river snail powder and bullfrog feed additive, crushing and sieving with a sieve of 100 meshes and 120 meshes to obtain the bullfrog feed.
10. The use of a bullfrog feed additive as claimed in claim 9, wherein: 10-20 parts of fish meal, 30-40 parts of soybean meal, 5-10 parts of corn flour, 10-15 parts of wheat bran, 10-15 parts of seaweed powder, 5-10 parts of rice bran, 5-10 parts of viviparidae powder and 20-25 parts of bullfrog feed additive.
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| CN201911127897.3A CN110679795A (en) | 2019-11-18 | 2019-11-18 | Bullfrog feed additive and preparation method and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911127897.3A CN110679795A (en) | 2019-11-18 | 2019-11-18 | Bullfrog feed additive and preparation method and application thereof |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4341800A (en) * | 1980-12-01 | 1982-07-27 | Dozier Lester | Food for frogs |
| CN105519774A (en) * | 2014-09-28 | 2016-04-27 | 孙丽君 | Special rana catesbeiana matching feed and processing method thereof |
| CN106333073A (en) * | 2016-08-26 | 2017-01-18 | 滁州菲扬农业科技有限公司 | Healthy feed additive for bullfrog and preparation method thereof |
| CN108056233A (en) * | 2017-12-13 | 2018-05-22 | 杨凤霞 | A kind of herbal medicine feed addictive and preparation method for promoting bullfrog growth |
| CN109497295A (en) * | 2018-11-13 | 2019-03-22 | 南宁学院 | A kind of feed addictive and preparation method thereof enhancing bullfrog immunity |
-
2019
- 2019-11-18 CN CN201911127897.3A patent/CN110679795A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4341800A (en) * | 1980-12-01 | 1982-07-27 | Dozier Lester | Food for frogs |
| CN105519774A (en) * | 2014-09-28 | 2016-04-27 | 孙丽君 | Special rana catesbeiana matching feed and processing method thereof |
| CN106333073A (en) * | 2016-08-26 | 2017-01-18 | 滁州菲扬农业科技有限公司 | Healthy feed additive for bullfrog and preparation method thereof |
| CN108056233A (en) * | 2017-12-13 | 2018-05-22 | 杨凤霞 | A kind of herbal medicine feed addictive and preparation method for promoting bullfrog growth |
| CN109497295A (en) * | 2018-11-13 | 2019-03-22 | 南宁学院 | A kind of feed addictive and preparation method thereof enhancing bullfrog immunity |
Non-Patent Citations (2)
| Title |
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| 卫强: "《植物茎叶化学成分的提取分离及活性研究》", 30 September 2018, 安徽大学出版社 * |
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