CN110664863A - Three-phase distribution method based on integration of extraction and separation of active substances of eucommia ulmoides leaves - Google Patents

Three-phase distribution method based on integration of extraction and separation of active substances of eucommia ulmoides leaves Download PDF

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CN110664863A
CN110664863A CN201911103170.1A CN201911103170A CN110664863A CN 110664863 A CN110664863 A CN 110664863A CN 201911103170 A CN201911103170 A CN 201911103170A CN 110664863 A CN110664863 A CN 110664863A
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王润平
杨再波
吕文志
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Qiannan Normal University for Nationalities
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
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    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization

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Abstract

The invention discloses a three-phase distribution method based on the integration of extraction and separation of active substances from eucommia leaves, which comprises the following steps: 1) adding the eucommia ulmoides leaf powder into a choline tryptophan ionic liquid aqueous solution, leaching, and centrifuging to remove insoluble substances; 2) will K3PO4Adding the aqueous solution into the solution obtained in the step 1), uniformly stirring, and standing until three phases are formed; 3) carrying out layered separation on the solution subjected to phase separation in the step 2), wherein the lower layer is inorganic salt K3PO4Enriching the phase, precipitating polysaccharide by adding ethanol, and recovering ethanol by rotary evaporation to obtain polysaccharide; the middle layer is a protein enrichment phase and is directly separated and recovered; the upper layer is choline tryptophan ionic liquid enrichment phase, chlorogenic acid and brass in the choline tryptophan ionic liquid enrichment phase are precipitated after the anti-solvent is added, and then the anti-solvent is recovered through rotary evaporation, so that the chlorogenic acid and the brass are obtained. The invention can treat protein, brass and chlorogenic acid in eucommia leavesThe method has the advantages of integrated extraction and separation, simple process, mild conditions, short separation time and high efficiency.

Description

Three-phase distribution method based on integration of extraction and separation of active substances of eucommia ulmoides leaves
Technical Field
The invention relates to an extraction and separation method of eucommia ulmoides leaf active substances, in particular to a three-phase distribution method based on the integration of extraction and separation of eucommia ulmoides leaf active substances.
Background
Eucommia bark is a traditional Chinese medicinal material, is used as a medicine from old times, has the effects of tonifying liver and kidney, strengthening bones and muscles, reducing blood pressure, preventing miscarriage and the like, has unique curative effect on hypertension, and is considered to be one of the most ideal natural blood pressure reducing medicines. Modern researches also show that eucommia bark has the effects of reducing blood pressure, regulating immunity, resisting viruses, resisting tumors and the like. The eucommia ulmoides leaves have high yield, and the collection of the eucommia ulmoides leaves does not influence the growth of eucommia ulmoides trees, so the research and application of the eucommia ulmoides leaves have great potential value. Meanwhile, the modern analysis means researches prove that the medicinal effective components of the eucommia ulmoides leaves are basically the same as the bark, and the medicinal functions are basically consistent. At present, more than 70 organic substances are separated and identified in folium cortex eucommiae, besides, various mineral substances also exist, and the folium cortex eucommiae can be roughly divided into lignans, iridoids, phenylpropanoids, flavonoids, polysaccharides, gutta percha, amino acids, volatile oil, trace elements, vitamins and the like according to the structural characteristics of the folium cortex eucommiae. Therefore, the method has important application significance in the research of the extraction and separation of the active substances of the eucommia ulmoides leaves.
The ionic liquid aqueous two-phase (ILATPS) has the advantages of low energy consumption, short process time, reliable scalability, and biocompatible environment, and is therefore considered as an economical and efficient downstream processing method. Moreover, ILATPS exhibits lower viscosity, does not form opaque aqueous solutions, and solutions have a wider range of polarity than other aqueous two-phase systems (based on polymer-polymer, polymer-salt, or salt-salt systems); this novel technique has therefore been applied to the extraction of different types of biomolecules, such as antibiotics, amino acids, proteins, hormones and alkaloids. Nevertheless, few studies have been directed to the stripping step that may be required for the isolation of target biomolecules, which may be a major challenge that must be overcome later.
In response to the limitations presented by ionic liquid aqueous two-phase (ILATPS), ionic liquid-based three-phase partitioning (ILTPP) began to emerge as a promising technology for protein recovery. In the ILTPP technique, protein in the solid phase accumulates between the liquid-liquid interfaces of the ionic liquid rich phase and the salt rich phase. The technology combines the advantages of ionic liquid aqueous two-phase systems (ILATPS) and three-phase distribution (TPP), and can obtain the target compound at the two-phase interface of the corresponding ionic liquid/salt aqueous two-phase systems, which is the characteristic of TPP. ILTPP has been successfully applied in the recovery of bovine whey proteins with important nutritional properties, such as lactoferrin, where an ionic liquid/salt system consisting of 1-butyl-3-methylimidazolium trifluoromethanesulfonic acid and phosphate is considered to be the most suitable for three-phase partitioning.
The economic and environmental aspects involved in ionic liquid based three-phase dispensing are highly dependent on the consumption of the reagents used, especially ionic liquids, which may sometimes be more effective than some volatile organic solvents due to their relatively high price and environmental impact. From previous studies it can be concluded that 0.8-5% of the ionic liquid cannot be reused during the use of the ILTPP, depending on the experimental conditions at the time. Although these lost ionic liquids are of little value, they can result in significant economic and environmental impact, since ionic liquids are one of the major constituents, accounting for over 20% of the ILTPP system; and the biodegradability and the biocompatibility of the commonly used traditional imidazole ionic liquid are poor. Therefore, corresponding measures should be taken in the process to enhance the recovery of the ionic liquid.
The related documents of ionic liquid three-phase partitioning (ILTPP) reported at present are all based on traditional imidazole ionic liquids, and generally relate to the separation of some model protein molecules, and the separation of choline amino acid ionic liquids with better biocompatibility and biodegradability and proteins in complex plant matrixes is not related at present.
Disclosure of Invention
The invention aims to provide a three-phase distribution method based on the integration of extraction and separation of active substances of eucommia ulmoides leaves. The method can be used for integrally extracting and separating the protein, the brass and the chlorogenic acid in the eucommia leaves, and has the advantages of simple process, mild condition, short separation time and high efficiency.
The technical scheme of the invention is as follows: a three-phase distribution method based on the integration of extraction and separation of active substances of eucommia ulmoides leaves comprises the following steps:
1) taking choline tryptophan ionic liquid ([ Ch)][Trp]) And inorganic salt K3PO4And is ready for use;
2) determination of choline tryptophan ionic liquid and inorganic salt K by cloud point method and weight method respectively3PO4The phase diagram and the line of the formed ionic liquid aqueous two-phase at 25 ℃;
3) adding the eucommia ulmoides leaf powder into a choline tryptophan ionic liquid aqueous solution, leaching for 40-80min, and then centrifuging to remove insoluble substances;
4) according to the phase diagram and the series line data of the step 2), adding the inorganic salt K3PO4Adding the aqueous solution into the solution obtained in the step 3), uniformly stirring, and standing until the solution phase is separated and balanced to form three phases;
5) carrying out layered separation on the solution subjected to phase separation in the step 4), wherein the lower layer is inorganic salt K3PO4Enriching the phase, precipitating polysaccharide by adding ethanol, and recovering ethanol by rotary evaporation to obtain polysaccharide; the middle layer is a protein enrichment phase and is directly separated and recovered; the upper layer is choline tryptophan ionic liquid enrichment phase, chlorogenic acid and brass in the choline tryptophan ionic liquid enrichment phase are precipitated after the anti-solvent is added, and then the anti-solvent is recovered through rotary evaporation, so that the chlorogenic acid and the brass are obtained.
In the above three-phase distribution method based on the integration of extraction and separation of active ingredients from folium Eucommiae, the folium Eucommiae powder is prepared by washing folium Eucommiae with tap water, air drying, and pulverizing into 0.1-1cm2Drying the small blocks in an oven at the temperature of 30-50 ℃ for 1-3h to obtain the food.
In the three-phase distribution method based on the integration of extraction and separation of active substances from eucommia ulmoides leaves, the concentration of the aqueous solution of the choline tryptophan ionic liquid in the step 3) is 35-50%.
In the aforementioned three-phase distribution method based on the integration of extraction and separation of active substances from folium cortex eucommiae, in step 3), the solid-to-liquid ratio of the folium cortex eucommiae powder to the choline tryptophan ionic liquid aqueous solution is 1: 5-25.
In the aforementioned three-phase distribution method based on the integration of extraction and separation of active ingredients from folium cortex eucommiae, in the step 3), the temperature for extraction is 20-70 ℃.
In the aforementioned three-phase distribution method based on the integration of extraction and separation of active ingredients from folium cortex eucommiae, in step 4), inorganic salt K is added3PO4The mass ratio of the water solution to the folium Eucommiae powder is 10-32:1-3, and inorganic salt K3PO4The concentration of the aqueous solution was 45%.
The invention has the advantages of
The invention can carry out integrated extraction and separation on the protein, the brass and the chlorogenic acid in the eucommia leaves by the process, and the whole process has the advantages of simple process, mild condition, short separation time and high efficiency.
Drawings
FIG. 1 is [ Ch][Trp]+K3PO4+ H2Phase diagram and line of O ionic liquid aqueous two-phase system;
fig. 2 is a flow chart of the present invention.
Detailed Description
The present invention is further illustrated by the following examples, which are not to be construed as limiting the invention.
Examples of the invention
Example 1:
(1) 1.3g of a sample of eucommia ulmoides leaf powder (particle size 0.5 cm) was accurately weighed2About) the sample is put into a 10ml centrifuge tube, 8g of choline tryptophan ionic liquid aqueous solution with the concentration of 35 percent is added, the extraction is carried out for 80min at the temperature of 20 ℃, and then the insoluble sample tissue is removed by centrifugation;
(2) 8g of 45% strength inorganic salt K3PO4Adding the aqueous solution into the centrifuged supernatant, uniformly stirring and standing for 2 hours; after the phase separation of the mixed solution reaches balance, the whole system is divided into three phases;
(3) the three phases, i.e., the upper phase (ionic liquid-enriched phase), the middle phase (leaf protein-precipitated phase) and the lower phase (K) were recorded separately3PO4Enriched phase), the volume of the upper phase was 2.7ml, the volume of the middle phase was 0.3ml and the volume of the lower phase was 6.3 ml; respectively sucking out the lower phase and the upper phase by using an injector to separate the three phases;
(4) directly separating and recovering the intermediate solid phase, namely the protein-enriched phase, wherein the yield of the protein extract is 0.25ml/g of folium cortex eucommiae, and the protein content is 1.6 mg/ml;
(5) separating chlorogenic acid, flavone, etc. in the upper phase by anti-solvent precipitation, wherein the contents of chlorogenic acid and total flavone are respectively 5mg/ml and 20mg/ml, recovering anti-solvent by rotary evaporation, and simultaneously recovering choline tryptophan ion liquid solution for reuse;
(6) separating polysaccharide in lower phase (inorganic salt enriched phase) by ethanol precipitation to obtain polysaccharide yield of 20mg/ml, recovering ethanol by rotary evaporation, and recovering K3PO4The solution can be recovered for reuse.
Example 2:
(1) a sample of eucommia ulmoides leaf powder (particle size 0.1 cm) was accurately weighed at 0.3g2About) the sample is put into a 10ml centrifuge tube, 8g of choline tryptophan ionic liquid aqueous solution with the concentration of 38 percent is added, and 50 g of the choline tryptophan ionic liquid aqueous solutionoC leaching for 60min, and then centrifuging to remove insoluble sample tissues;
(2) 8g of 45% strength inorganic salt K3PO4Adding the aqueous solution into the centrifuged supernatant, uniformly stirring and standing for 2 hours; after the phase separation of the mixed solution reaches balance, the whole system is divided into three phases;
(3) the three phases, i.e., the upper phase (ionic liquid-enriched phase), the middle phase (leaf protein-precipitated phase) and the lower phase (K) were recorded separately3PO4Enriched phase), the volume of the upper phase was 2.8ml, the volume of the middle phase was 0.6ml and the volume of the lower phase was 7.8 ml; respectively sucking out the lower phase and the upper phase by using an injector to separate the three phases;
(4) directly separating and recovering the intermediate solid phase, namely the protein-enriched phase, wherein the yield of the protein extract is 2ml/g of folium cortex eucommiae, and the protein content is 1.5 mg/ml;
(5) separating chlorogenic acid, flavone, etc. in the upper phase by anti-solvent precipitation, wherein the contents of chlorogenic acid and total flavone are respectively 2mg/ml and 10mg/ml, recovering anti-solvent by rotary evaporation, and simultaneously recovering choline tryptophan ion liquid solution for reuse;
(6) the polysaccharide in the lower phase, i.e. the inorganic salt-enriched phase, is separated by ethanol precipitation, the yield of the polysaccharide is 12mg/ml, and the method is implementedRotary evaporation to recover ethanol while K3PO4The solution can be recovered for reuse.
Example 3:
(1) a0.3 g sample of powdered eucommia ulmoides leaf powder (particle size 1 cm) was accurately weighed2About) the sample is put into a 10ml centrifuge tube, 8g of choline tryptophan ionic liquid aqueous solution with the concentration of 50 percent is added, 60 g of the choline tryptophan ionic liquid aqueous solution is addedoC leaching for 40min, and then centrifuging to remove insoluble sample tissues;
(2) 8g of 45% strength inorganic salt K3PO4Adding the aqueous solution into the centrifuged supernatant, uniformly stirring and standing for 2 hours; after the phase separation of the mixed solution reaches balance, the whole system is divided into three phases;
(3) the three phases, i.e., the upper phase (ionic liquid-enriched phase), the middle phase (leaf protein-precipitated phase) and the lower phase (K) were recorded separately3PO4Enriched phase), the volume of the upper phase was 3.0ml, the volume of the middle phase was 1.4ml and the volume of the lower phase was 6.6 ml; respectively sucking out the lower phase and the upper phase by using an injector to separate the three phases;
(4) directly separating and recovering the intermediate solid phase, namely the protein-enriched phase, wherein the yield of the protein extract is 4.5ml/g of folium cortex eucommiae, and the protein content is 1.5 mg/ml;
(5) separating chlorogenic acid, flavone, etc. in the upper phase by anti-solvent precipitation, wherein the contents of chlorogenic acid and total flavone are respectively 2.5mg/ml and 12mg/ml, recovering anti-solvent by rotary evaporation, and simultaneously recovering choline tryptophan ionic liquid solution for reuse;
(6) separating polysaccharide in lower phase, i.e. inorganic salt-enriched phase, by ethanol precipitation to obtain polysaccharide yield of 15mg/ml, recovering ethanol by rotary evaporation, and recovering K3PO4The solution can be recovered for reuse.
Example 4: a three-phase distribution method based on the integration of extraction and separation of active substances of eucommia leaves comprises the following steps:
1) taking choline tryptophan ionic liquid and inorganic salt K3PO4And is ready for use;
2) determination of choline tryptophan ionic liquid and inorganic salt K by cloud point method and weight method respectively3PO4The phase diagram and the line of the formed ionic liquid aqueous two-phase at 25 ℃;
3) cleaning folium Eucommiae with tap water, air drying, and pulverizing into 0.1-1cm2Drying the small blocks in an oven at 30-50 deg.C for 1-3h to obtain folium Eucommiae powder, adding 35-50% choline tryptophan ionic liquid water solution (solid-to-liquid ratio of folium Eucommiae powder to choline tryptophan ionic liquid water solution is 1: 5-25), leaching for 40-80min at 20-70 deg.C, and centrifuging to remove insoluble substances;
4) according to the phase diagram and the series line data of the step 2), adding the inorganic salt K3PO4Adding the aqueous solution into the solution obtained in step 3), and adding inorganic salt K3PO4The mass ratio of the water solution to the folium Eucommiae powder is 10-32:1-3, and inorganic salt K3PO4The concentration of the aqueous solution is 45 percent, and after the aqueous solution is uniformly stirred, the aqueous solution is kept stand until the solution phase is separated and balanced to form three phases;
5) carrying out layered separation on the solution subjected to phase separation in the step 4), wherein the lower layer is inorganic salt K3PO4Enriching the phase, precipitating polysaccharide by adding ethanol, and recovering ethanol by rotary evaporation to obtain polysaccharide; the middle layer is a protein enrichment phase and is directly separated and recovered; the upper layer is choline tryptophan ionic liquid enrichment phase, chlorogenic acid and brass in the choline tryptophan ionic liquid enrichment phase are precipitated after the anti-solvent is added, and then the anti-solvent is recovered through rotary evaporation, so that the chlorogenic acid and the brass are obtained.
Example 4: a three-phase distribution method based on the integration of extraction and separation of active substances of eucommia ulmoides leaves comprises the following steps:
1) taking choline tryptophan ionic liquid and inorganic salt K3PO4And is ready for use;
2) determination of choline tryptophan ionic liquid and inorganic salt K by cloud point method and weight method respectively3PO4The phase diagram and the line of the formed ionic liquid aqueous two-phase at 25 ℃;
3) cleaning folium Eucommiae with tap water, air drying, and pulverizing into 0.1cm2Drying the small pieces in 30 deg.C oven for 1 hr to obtain folium Eucommiae powder, adding 35% choline tryptophan ionic liquid water solution (the folium Eucommiae powder and choline tryptophan ionic liquid are dissolved in water)The solid-liquid ratio of the liquid is 1: 5), leaching for 40min at the temperature of 20 ℃, and then centrifuging to remove insoluble substances;
4) according to the phase diagram and the series line data of the step 2), adding the inorganic salt K3PO4Adding the aqueous solution into the solution obtained in step 3), and adding inorganic salt K3PO4The mass ratio of the aqueous solution to the folium Eucommiae powder is 10:1, and inorganic salt K3PO4The concentration of the aqueous solution is 45 percent, and after the aqueous solution is uniformly stirred, the aqueous solution is kept stand until the solution phase is separated and balanced to form three phases;
5) carrying out layered separation on the solution subjected to phase separation in the step 4), wherein the lower layer is inorganic salt K3PO4Enriching the phase, precipitating polysaccharide by adding ethanol, and recovering ethanol by rotary evaporation to obtain polysaccharide; the middle layer is a protein enrichment phase and is directly separated and recovered; the upper layer is choline tryptophan ionic liquid enrichment phase, chlorogenic acid and brass in the choline tryptophan ionic liquid enrichment phase are precipitated after the anti-solvent is added, and then the anti-solvent is recovered through rotary evaporation, so that the chlorogenic acid and the brass are obtained.
Example 5: a three-phase distribution method based on the integration of extraction and separation of active substances of eucommia leaves comprises the following steps:
1) taking choline tryptophan ionic liquid and inorganic salt K3PO4And is ready for use;
2) determination of choline tryptophan ionic liquid and inorganic salt K by cloud point method and weight method respectively3PO4The phase diagram and the line of the formed ionic liquid aqueous two-phase at 25 ℃;
3) cleaning folium Eucommiae with tap water, air drying, and pulverizing into 1cm2Drying the small blocks in a 50 ℃ oven for 3h to obtain eucommia leaf powder, adding 50% choline tryptophan ionic liquid aqueous solution (the solid-to-liquid ratio of the eucommia leaf powder to the choline tryptophan ionic liquid aqueous solution is 1: 25), leaching for 80min at 70 ℃, and centrifuging to remove insoluble substances;
4) according to the phase diagram and the series line data of the step 2), adding the inorganic salt K3PO4Adding the aqueous solution into the solution obtained in step 3), and adding inorganic salt K3PO4The mass ratio of the aqueous solution to the eucommia ulmoides leaf powder is 32: 3, and the inorganic salt K3PO4The concentration of the aqueous solution is 45 percent, and after the aqueous solution is uniformly stirred, the aqueous solution is kept stand until the solution phase is separated and balanced to form three phases;
5) carrying out layered separation on the solution subjected to phase separation in the step 4), wherein the lower layer is inorganic salt K3PO4Enriching the phase, precipitating polysaccharide by adding ethanol, and recovering ethanol by rotary evaporation to obtain polysaccharide; the middle layer is a protein enrichment phase and is directly separated and recovered; the upper layer is choline tryptophan ionic liquid enrichment phase, chlorogenic acid and brass in the choline tryptophan ionic liquid enrichment phase are precipitated after the anti-solvent is added, and then the anti-solvent is recovered through rotary evaporation, so that the chlorogenic acid and the brass are obtained.
The above description is only for the purpose of illustrating the present invention and the appended claims, and the scope of the present invention is not limited thereto, and any person skilled in the art can substitute or change the technical solution and the inventive concept of the present invention within the technical scope of the present invention.

Claims (6)

1. A three-phase distribution method based on the integration of extraction and separation of active substances of eucommia ulmoides leaves is characterized by comprising the following steps of:
1) taking choline tryptophan ionic liquid and inorganic salt K3PO4And is ready for use;
2) determination of choline tryptophan ionic liquid and inorganic salt K by cloud point method and weight method respectively3PO4The phase diagram and the line of the formed ionic liquid aqueous two-phase at 25 ℃;
3) adding the eucommia ulmoides leaf powder into a choline tryptophan ionic liquid aqueous solution, leaching for 40-80min, and then centrifuging to remove insoluble substances;
4) according to the phase diagram and the series line data of the step 2), adding the inorganic salt K3PO4Adding the aqueous solution into the solution obtained in the step 3), uniformly stirring, and standing until the solution phase is separated and balanced to form three phases;
5) carrying out layered separation on the solution subjected to phase separation in the step 4), wherein the lower layer is inorganic salt K3PO4Enriching the phase by adding ethanol to itPrecipitating polysaccharide, and recovering ethanol by rotary evaporation to obtain polysaccharide; the middle layer is a protein enrichment phase and is directly separated and recovered; the upper layer is choline tryptophan ionic liquid enrichment phase, chlorogenic acid and brass in the choline tryptophan ionic liquid enrichment phase are precipitated after the anti-solvent is added, and then the anti-solvent is recovered through rotary evaporation, so that the chlorogenic acid and the brass are obtained.
2. The eucommia ulmoides leaf active matter extraction and separation integrated three-phase distribution method according to claim 1, wherein: the folium Eucommiae powder is prepared by cleaning with tap water, air drying, and pulverizing into 0.1-1cm powder2Drying the small blocks in an oven at the temperature of 30-50 ℃ for 1-3h to obtain the food.
3. The eucommia ulmoides leaf active matter extraction and separation integrated three-phase distribution method according to claim 1, wherein: and 3) the concentration of the choline tryptophan ionic liquid aqueous solution in the step 3) is 35-50%.
4. The eucommia ulmoides leaf active matter extraction and separation integrated three-phase distribution method according to claim 1, wherein: in the step 3), the solid-to-liquid ratio of the eucommia ulmoides leaf powder to the choline tryptophan ionic liquid aqueous solution is 1: 5-25.
5. The eucommia ulmoides leaf active matter extraction and separation integrated three-phase distribution method according to claim 1, wherein: in the step 3), the temperature for leaching is 20-70 ℃.
6. The eucommia ulmoides leaf active matter extraction and separation integrated three-phase distribution method according to claim 1, wherein: in the step 4), the inorganic salt K is added3PO4The mass ratio of the water solution to the folium Eucommiae powder is 10-32:1-3, and inorganic salt K3PO4The concentration of the aqueous solution was 45%.
CN201911103170.1A 2019-11-12 2019-11-12 Three-phase distribution method based on integration of extraction and separation of active substances of eucommia ulmoides leaves Withdrawn CN110664863A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115594773A (en) * 2022-09-22 2023-01-13 黔南民族师范学院（Cn） Eucommia ulmoides leaf extraction method with high polysaccharide yield and application thereof
CN116284465A (en) * 2022-08-05 2023-06-23 西北农林科技大学 Preparation method of eucommia ulmoides polysaccharide and application of eucommia ulmoides polysaccharide prepared by preparation method in anti-depression
CN116422002A (en) * 2023-01-13 2023-07-14 完美（广东）日用品有限公司 Aloe extract and preparation method and application thereof

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116284465A (en) * 2022-08-05 2023-06-23 西北农林科技大学 Preparation method of eucommia ulmoides polysaccharide and application of eucommia ulmoides polysaccharide prepared by preparation method in anti-depression
CN115594773A (en) * 2022-09-22 2023-01-13 黔南民族师范学院（Cn） Eucommia ulmoides leaf extraction method with high polysaccharide yield and application thereof
CN116422002A (en) * 2023-01-13 2023-07-14 完美（广东）日用品有限公司 Aloe extract and preparation method and application thereof
CN116422002B (en) * 2023-01-13 2023-10-20 完美（广东）日用品有限公司 Aloe extract and preparation method and application thereof

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