CN110663694A

CN110663694A - Cyantraniliprole and cyromazine compound insecticide

Info

Publication number: CN110663694A
Application number: CN201911059998.1A
Authority: CN
Inventors: 刘乐; 马碧峰; 李建群; 张建华
Original assignee: Hunan New Changshan Agriculture Development Ltd By Share Ltd
Current assignee: Hunan New Changshan Agriculture Development Ltd By Share Ltd
Priority date: 2019-11-01
Filing date: 2019-11-01
Publication date: 2020-01-10

Abstract

The invention discloses an insecticide composition of cyromazine and cyantraniliprole, wherein the mass ratio of cyromazine to cyromtraniliprole is 1-20: 1, and the balance is auxiliary components acceptable in pesticide preparations. The insecticidal composition of the invention is used for controlling lepidoptera pests, in particular pieridae pests such as cabbage caterpillars. Compared with the prior art, the composition has obvious synergistic effect and improves the control effect on pests; the generation of drug resistance of pests is delayed, and the life cycle of the product is prolonged.

Description

Cyantraniliprole and cyromazine compound insecticide

Technical Field

The invention relates to the field of compound pesticides, in particular to compound of two pesticides.

Background

Cyantraniliprole is a second generation of ryanodine receptor inhibitor insecticides successfully developed by DuPont company following chlorantraniliprole (trade name "kangba"), the common name of which is Cyantraniliprole, the chemical name of which is 3-bromo-1- (3-chloro-2-pyridyl) -N- [ 4-cyano-2-methyl-6- [ (methylamino) carbonyl ] phenyl ] -1H-pyrazole-5-carboxamide, and the molecular structural formula is as follows:

cyantraniliprole controls pests by activating the ryanodine receptor of the target pest. Activation of the ryanodine receptor releases calcium ions stored in smooth muscle and striated muscle cells, which results in impaired muscle motor regulation, paralysis and ultimately death of the pest. The drug shows extremely obvious selectivity difference to mammals and pests, namely the ryanodine receptor, and greatly improves the safety to the mammals, other vertebrates and other natural enemies. Can be used for preventing and treating pests of vegetables, fruit trees and various farmland crops, such as cabbage looper, cabbage aphid, prodenia litura, twenty-eight star ladybug, whitefly, cotton bollworm, red spider, aphid, striped flea beetle, etc., and can be used for preventing and treating underground pests of cutworm, wireworm, mole cricket, grub, ground maggot, nematode, etc.

Cyromazine, also known as Cyromazine, has the chemical name of N-cyclopropyl-1, 3, 5-triazine-2, 4, 6-triamine and the molecular structural formula as follows:

is a triazine insect growth regulator, has special activity on dipteran larva, and can induce dipteran larva and pupa to have morphological aberration, and adult eclosion is incomplete or inhibited. Is used for controlling flies in animal stable houses and controlling agricultural pests such as Liriomyza sativae on cucumbers, eggplants, kidney beans, leaf vegetables and flowers.

A mixture in which two or more kinds of pesticides having different mechanisms of action or control targets are mixed together for a certain purpose is called a built pesticide or a mixed preparation. Development of new agricultural compounds is costly and long-lasting. In contrast, compounding and blending have the advantages of small investment and short development period, and are paid more attention at home and abroad, and development strength is increased at a glance. The scientific and reasonable pesticide compounding or blending has the advantages of improving the control effect, prolonging the pesticide application period, reducing the dosage, reducing the risk of phytotoxicity, reducing residues, delaying the occurrence and development of drug resistance and the like. However, the action of the compounded pesticide on organisms (including control objects, protected objects, human beings and livestock and the like) is not always one plus one or two, and may be enhanced or reduced. (1) The combined effect of the additive pesticide on the same organism is additive effect, i.e. additive effect, when the sum of the toxicity of the additive pesticide on the same organism is equal to the sum of the toxicity of the individual agents of the mixture. (2) Synergistic effect the combined effect of the mixed preparation is synergistic when the toxicity of the mixed preparation to the same organism is larger than the sum of the toxicity of the single preparations of the mixed preparation, and the synergistic effect changes along with the change of the mixture ratio. (3) The toxicity of the antagonism compound mixture to the same organism is obviously reduced than the sum of the toxicity of each medicament which forms the mixture and is used alone, which is called antagonism. The antagonism is shown in that the prevention and control effect is reduced, and the pesticide damage to protected objects is reduced.

The pieris rapae, also called pieris rapae, and larvae are also called pieris rapae, belong to lepidoptera pieris, and are pests which are most commonly distributed in China, have the most serious harm and are frequently damaged. Known host plants are as many as 35 of the 9 families, and are addicted to cruciferous plants, particularly thick leaf cabbages, cauliflowers, bok choy, radishes, and the like. In the absence of cruciferous plants, other host plants may also be consumed, such as plants of Compositae, Capparidaceae, globeflower, Liliaceae, Boraginaceae, Oleaceae, etc.

Disclosure of Invention

The inventor finds that no compound insecticide of cyanogenamide perbromide and cyromazine is disclosed in the prior art. On the basis of the intensive research on the compounded insecticide of cyantraniliprole and cyromazine, the inventor finds that the compounding of the cyantraniliprole and the cyromazine has obvious synergistic effect in a certain proportion range, thereby completing the invention.

The invention provides a pesticide composition of cyromazine and cyantraniliprole, which comprises the following components in percentage by mass: 1-20: 1, preferably 3-15: 1, further preferably 3-6: 1, for example 6:1 or 5:1, most preferably 3.5-5.5: 1. The mass percentage of the active components (cyromazine and cyantraniliprole) in the composition is 3-90%, and the balance is acceptable auxiliary components in pesticide preparations. The auxiliary components comprise known substances which are beneficial to stabilizing and playing the efficacy of effective components in the preparation, such as a solvent, an emulsifier, a wetting agent, a stabilizer, a dispersing agent, a thickening agent, a pH regulator, an antifoaming agent, an antifreezing agent, a filler and the like, and the specific components and the dosage can be determined through experiments according to the formula requirements.

The pesticide composition can be prepared into various conventional formulations suitable for agriculture by a known method, such as suspending agents, water dispersible granules, emulsion in water, missible oil, microemulsion, wettable powder and the like. The production process of various conventional preparations belongs to the prior art, and is not described in detail herein.

The insecticidal composition of the invention is used for controlling lepidoptera pests, in particular pieridae pests such as cabbage caterpillars. Compared with the prior art, the invention has the beneficial effects that: 1. the cyromazine and the cyantraniliprole are compounded to have obvious synergistic effect, the control effect on pests is improved, the using amount of pesticides is reduced, the use cost is reduced, and the pollution to the environment is reduced. 2. Because the dosage is reduced and the action mechanisms of the cyromazine and the cyantraniliprole are different, the generation of drug resistance of pests can be delayed and the life cycle of pesticide products can be prolonged.

Detailed Description

The following description is only a preferred embodiment of the present invention, and is only for the purpose of describing the present invention, and should not be construed as limiting the scope of the present invention. It should be understood that any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Example 1 indoor Combined virulence assay

The cabbage caterpillar (Pierisrapae Linne) as insect source to be tested is collected from the cabbage field of Pinmura village in hibiscus area of Changsha city, Hunan province, and is bred indoors by using water culture cabbage seedlings. All the larvae are raised to 3 rd generation larvae for later use in a larva raising room with the insect state of (28 +/-l) DEG C, the photoperiod of 14h/10h (light/dark) and the environmental humidity of RH 50-75%.

Instrument and apparatus, and reagent

Electronic balance (sensory 0.1 mg); a glass slide; a capillary tube; filtering paper; a writing brush; a petri dish with a diameter of 9 cm; a beaker; a pipette; tweezers, etc.;

98% cyromazine as a technical material;

94% cyantraniliprole (cyantraniliprole) technical;

firstly, acetone is used to dissolve a proper amount of raw medicines and two raw medicines in different proportions and the raw medicines are diluted into 5 series of concentration gradients for standby. Diluting cyromazine to 16, 8, 4, 2 and 1 mg/L; diluting cyantraniliprole to 8, 4, 2, 1 and 0.5 mg/L; the 5 cyromazine and the cyantraniliprole with different proportions (the effective components are respectively 15:1,7.5:1,5:1, 3.75:1 and 3:1) are respectively diluted into 10, 5, 2.5, 1.25 and 0.625mg/L series concentrations for standby.

The drug treatment was measured by the leaf blade method. Beating cabbage butterfly with diameter of 1cm with puncher, placing into culture dish, and keeping moisture. Dripping 1-2 mu L of liquid medicine into each leaf disk by using a capillary dropper from low concentration, folding the leaf disk with another leaf disk coated with starch paste after a solvent is volatilized to prepare a virus-containing leaf disk, and putting the virus-containing leaf disk into holes of a 12-hole tissue culture plate after the preparation is finished. Each treatment was repeated 4 times, each time for not less than 12 toxic leaf discs, and treatment without drug but with the corresponding organic solvent was used as a control. 1 test insect is inoculated into each hole of the tissue culture plate and cultured under normal conditions. After the test insects are inoculated for 2-4 h, after the test insects eat the drug-containing leaf discs, clean feed is added into the holes of the culture plate and continuously raised until investigation, and the test insects which do not eat a complete leaf disc are eliminated. The number of test insects treated by each dose was 60.

And investigating the death condition of the test insects 24 hours after investigation treatment (judging the death standard of the test insects is that the needles are pricked lightly and do not have autonomous reaction). Total and dead worm numbers were recorded.

Data investigation and statistical analysis

Calculation method mortality in percent (%) was calculated for each treatment based on the survey data. Calculated according to the following formula, the calculation results are all reserved to the last two decimal points:

if the control mortality rate is less than 5%, not correcting; correcting according to a formula when the control mortality is between 5 and 20 percent; the control death rate is more than 20 percent, and the test needs to be redone.

Statistical analysis the statistical analysis was carried out using the DPS software to determine the virulence regression line (Y ═ a + bx) and the correlation coefficient (r) values, LC for the test agents₅₀、LC₉₀And the 95% confidence limit value and the like, and the co-toxicity coefficient of each mixing proportion is obtained by adopting a Sun cloud Peter method according to NY/T1154.7-2006, and the optimal proportion is selected.

The test results are according to NY/T1154.7-2006 pesticide combined action division standard: the co-toxicity coefficient (CTC) is more than or equal to 120 and shows a synergistic effect; CTC is less than or equal to 80 and shows antagonism; 80 < CTC < 120 showed additive effects. Toxicity of the cyromazine, the cyantraniliprole and 5 different mixed preparations is determined by the method, and the original data are shown in table 1. Statistical analysis was then performed and the results are shown in table 2. As can be seen from the data in the table, cyromazine: the co-toxicity coefficient of 5 different proportions (15:1,7.5:1,5:1, 3.75:1 and 3:1) of cyantraniliprole to cabbage caterpillar is more than 120, and the result shows that the two medicaments have obvious synergistic effect on preventing and controlling cabbage caterpillar after being compounded. The experiments show that cyromazine: when the mass ratio of the cyantraniliprole is 3.5-5.5: 1, the cotoxicity coefficient reaches a peak value interval and approaches to or even exceeds 300.

TABLE 1 toxicity assay original data table for cyromazine, cyantraniliprole and different ratios thereof to cabbage caterpillar

TABLE 2 toxicity test results of cyromazine, cyantraniliprole and cabbage caterpillar with 5 different matching ratios

EXAMPLE 2 suspending agent

The active ingredient formula comprises the following components: 3 percent of cyromazine, 3 percent of cyantraniliprole, 2 percent of fatty alcohol-polyoxyethylene ether, 1 percent of methylene dinaphthalene sodium sulfonate, 1.3 percent of calcium dodecyl benzene sulfonate, 3 percent of triphenylethylphenol polyoxyethylene ether, 1.5 percent of white carbon black, 0.25 percent of xanthan gum and the balance of water are complemented, and the suspending agent is prepared according to the conventional production mode of suspending agents. The percentages are all mass percentages.

Examples 3 to 9 suspending Agents

A suspension was prepared in a similar manner to example 1, with the same auxiliary ingredients but slightly different formulations of the active ingredients, as follows:

table 3 formula table of the examples

Example 10 field test

The cabbage caterpillar (Pierisrapae line) under the basic test condition is tested at a vegetable test base of Longzicun vegetable in Longmanomi of China, Changsha, Hunan province, spring China, and the town, according to normal conditions, the cabbage is cultivated, after three true leaves of the cabbage grow and the cabbage caterpillar occurs, an insect killing experiment is carried out, the pesticide application date is 2 months and 21 days, and the cabbage caterpillar head number is investigated at 2 months and 26 days, 3 months and 8 days and 3 months and 23 days.

Instrument and apparatus, and reagent

Electronic balance (sensory 0.1 mg); a hand-held sprayer; a measuring cylinder; a beaker; a pipette; tweezers; gloves and the like;

the suspending agents obtained in examples 4, 5 and 6;

the cabbage is treated by adopting a spraying method in the medicament treatment, the application amount of each embodiment is 100 ml/mu, the blank control is not applied, and the water consumption per mu is 35 liters.

Investigating the death condition of the test insects 5 days, 15 days and 30 days after the investigation treatment (judging the death standard of the test insects is that the needles are pricked lightly and do not have autonomous reaction, and recording the number of heads of the insects before application and the amount of residual insects after application.

Data investigation and result analysis

Table 4 comparative control of the examples

The field test data of the example 10 shows that compared with the example 4, the control effect of the cabbage caterpillar is better in the examples 5 and 6, and the relative control effect can reach about 90% in 30 days after the pesticide is applied. In addition, according to the statistical analysis of the test data, the co-toxicity coefficients of the examples 4 to 6 are all more than 200, and are basically consistent with the indoor combined virulence determination data of the example 1.

The invention has been described in detail with respect to a general description and specific embodiments thereof, but it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims

1. The pesticide composition of cyromazine and cyantraniliprole comprises cyromazine and cyantraniliprole in a mass ratio of 1-20: 1, and the balance of auxiliary components acceptable in pesticide preparations.

2. The insecticide composition of claim 1, wherein the mass ratio of cyromazine to cyantraniliprole is 3-15: 1.

3. The insecticide composition of claim 2, wherein the mass ratio of cyromazine to cyantraniliprole is 3-6: 1.

4. The insecticide composition of claim 3, wherein the mass ratio of cyromazine to cyantraniliprole is 3.5-5.5: 1.

5. The insecticide composition of claim 3, wherein the mass ratio of cyromazine to cyantraniliprole is 6:1 or 5: 1.

6. The pesticidal composition according to claim 1, which is a suspension.

7. The insecticide composition of claim 1, wherein the active ingredients (cyromazine and cyantraniliprole) are present in the composition in an amount of 3 to 90% by weight.

8. The pesticidal composition according to claim 1, which is used for controlling lepidopteran pests.

9. The pesticidal composition according to claim 8, which is used for controlling pests of the family pieridae.

10. The insecticide composition of claim 9, wherein said insecticide composition is used to control cabbage caterpillars.