CN110658234A - Disposable quick-dismantling type pollutant impedance detection device - Google Patents
Disposable quick-dismantling type pollutant impedance detection device Download PDFInfo
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- CN110658234A CN110658234A CN201910939672.1A CN201910939672A CN110658234A CN 110658234 A CN110658234 A CN 110658234A CN 201910939672 A CN201910939672 A CN 201910939672A CN 110658234 A CN110658234 A CN 110658234A
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- clamping plate
- reaction
- detection
- lower clamping
- reaction chamber
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
- G01N27/04—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance
Abstract
The invention discloses a disposable quick-dismantling type pollutant impedance detection device. The lower clamping plate and the upper clamping plate are vertically butted and installed into an integral shell and are fixedly connected through an elastic pressing assembly, and the reaction block is installed between the lower clamping plate and the upper clamping plate; the elastic pressing component comprises a pre-tightening spring, a pressing sliding block, an eccentric sleeve, a connecting rod and an adjusting nut; a cuboid space and a cylindrical space are arranged in the integral shell, a detection substrate is arranged in the cuboid space, two internal electrodes and two external electrodes are arranged on the detection substrate, and the two internal electrodes are electrically connected with the two external electrodes; the cylindrical space is used as a reaction chamber, the two inner electrodes are positioned in the reaction chamber, the two outer electrodes are positioned outside the reaction chamber, and the cylindrical space is provided with a sample adding hole. The invention realizes the impedance detection in the pollutant reaction liquid and can be widely used for pollutant detection in the fields of food safety, environmental detection and the like.
Description
Technical Field
The invention relates to the field of microbial detection, in particular to a device for detecting pollutants such as bacteria, fungi, viruses and the like based on an impedance method.
Background
Pathogenic bacteria are one of the important factors threatening food safety. The traditional bacteria detection method is a plate counting method. The method has long inspection period and cannot meet the requirement of rapid detection. The impedance method for detecting the total number of bacteria is to convert protein and carbohydrate in a culture medium into substances such as amino acid, lactic acid and the like according to the growth and metabolism of bacteria microorganisms so as to cause weak impedance change in the culture medium. The method has the advantages of rapid detection and accurate and reliable result, and can greatly shorten the detection period.
The existing bacteria detection technology usually adopts a colony counting method, the consumed time is long, the requirement on external detection equipment is higher, the volume of a reagent needing to be added in an experiment is relatively larger, the consumption of the reagent is increased, and the experiment cost is improved.
Disclosure of Invention
The invention aims to provide a portable and disposable device for detecting pollutants, and simultaneously, the device is combined with the characteristics of rapidness, sensitivity, simplicity and convenience of an impedance detection technology, monitors the impedance change in a reaction system in real time and indirectly detects target pollutants. When bacteria, fungi, viruses, etc. are propagated in the medium, the medium impedance changes due to the change in the distribution of the medium, and thus the detection can be performed by detecting the medium impedance.
In order to achieve the above purpose, the present invention mainly comprises:
the invention includes a device base, an internal electrode and an external electrode; the device base body comprises a lower clamping plate, a reaction block, an upper clamping plate and an elastic pressing assembly, the lower clamping plate and the upper clamping plate are vertically butted and installed into an integral shell, and the reaction block is installed between the lower clamping plate and the upper clamping plate; the lower clamping plate and the upper clamping plate are fixedly connected through an elastic pressing assembly, so that the reaction block is tightly clamped between the lower clamping plate and the upper clamping plate, the elastic pressing assembly comprises a pre-tightening spring, a pressing sliding block, an eccentric sleeve, a connecting rod and an adjusting nut, the bottom of the adjusting nut is fixed in a mounting counter bore formed in the lower clamping plate, the bottom end of the connecting rod is screwed into the adjusting nut through threads, the top end of the connecting rod is eccentrically hinged with the eccentric sleeve after penetrating through a through hole formed in the upper clamping plate, the pressing sliding block is sleeved outside the connecting rod below the eccentric sleeve, and the pre-tightening spring is sleeved outside the connecting rod; a horizontal flat cuboid space and a cylindrical space are arranged in the space between the reaction block and the lower clamping plate, and the cylindrical space is positioned above the cuboid space and is communicated with the cuboid space; a detection substrate is arranged in the cuboid space, two internal electrodes and two external electrodes are arranged on the upper surface of the detection substrate, and the two internal electrodes are respectively and electrically connected with the two external electrodes through respective wires arranged on the surface of the detection substrate; the cylindrical space is used as a reaction chamber, the reaction chamber is filled with reaction liquid, the two internal electrodes are positioned in the reaction chamber, the internal electrodes are directly contacted with the reaction liquid, the two external electrodes are positioned outside the reaction chamber, and the two external electrodes are led out and connected to an external detection instrument; the center of the top surface of the reaction block above the columnar space is provided with a sample adding hole for injecting reaction liquid, and the sample adding hole is sealed by sealant.
And a sealing ring is arranged between the bottom surface of the reaction block at the bottom of the reaction chamber and the detection substrate.
The shape and the size of the detection substrate are just matched with those of the cuboid space.
The side wall of the upper clamping plate around the reaction block is provided with a hollow structure, and the hollow structure is a plurality of through hollow grooves.
The detection substrate extends out of a surrounding shell formed between the reaction block and the lower clamping plate, and the two external electrodes are exposed out of the surrounding shell and then are electrically connected with an external detection instrument through respective wires.
The pollutants are liquid pollutants of bacteria, fungi and viruses.
The invention has the beneficial effects that:
the reaction device provided by the invention is used as a reaction device for providing a reaction space for pollutant detection, has small volume, and can realize high-sensitivity detection of a target object by combining an impedance detection mode. The device is small in reagent amount required in the experimental process, combines a front-end sensor, selects impedance detection as a detection means, is relatively less limited by the external environment, and can realize on-site rapid detection by combining a portable impedance detector. The quick-release structure can ensure the close attachment of the reaction device and the detection electrode, and the quick-release structure can quickly replace the reaction device, thereby facilitating the follow-up experiment.
Drawings
FIG. 1 is a block diagram of the apparatus of the present invention;
FIG. 2 is a block diagram of the resilient compression assembly of the present invention;
FIG. 3 is a sectional structural view of a reaction block of the present invention;
FIG. 4 is a view showing the construction of the lower splint of the present invention;
FIG. 5 is a structural view of the upper jaw of the present invention;
FIG. 6 is a schematic view of the structure of the detection substrate and the upper electrode thereof.
In the figure, a lower clamping plate 1, a reaction block 2, an upper clamping plate 3, a pre-tightening spring 4, a pressing sliding block 5, an eccentric sleeve 6, a connecting rod 7, a sealing ring 8, a detection substrate 9, an external electrode 10, an internal electrode 11, a sample adding hole 12, an adjusting nut 13 and a hollow structure 14.
Detailed Description
The present invention will be further described with reference to the drawings attached to the specification, but the present invention is not limited to the following examples.
As shown in fig. 1, the embodiment includes a device base, an inner electrode 11, and an outer electrode 10; the device base body comprises a lower clamping plate 1, a reaction block 2, an upper clamping plate 3 and an elastic pressing assembly. As shown in fig. 1 and 2, a lower clamping plate 1 and an upper clamping plate 3 are respectively assembled into an integral shell in a vertical butt joint mode, and a reaction block 2 is arranged between the lower clamping plate 1 and the upper clamping plate 3; the upper clamping plate 3 and the lower clamping plate 1 are installed, and the upper clamping plate 3 can play a good shading effect to form a darkroom. The lower clamping plate 1 and the upper clamping plate 3 are fixedly connected through an elastic pressing assembly, so that the reaction block 2 is clamped between the lower clamping plate 1 and the upper clamping plate 3.
As shown in fig. 2-4, the elastic pressing component comprises a pre-tightening spring 4, a pressing slider 5, an eccentric sleeve 6, a connecting rod 7 and an adjusting nut 13, the bottom of the adjusting nut 13 is fixed in a mounting counter bore formed in the lower clamp plate 1, the bottom end of the connecting rod 7 is screwed into the adjusting nut 13 through threads, the top end of the connecting rod 7 passes through a through hole formed in the upper clamp plate 3 and then is eccentrically hinged with the eccentric sleeve 6 through a horizontal shaft, a hinged shaft between the connecting rod 7 and the eccentric sleeve 6 deviates from the horizontal center of the eccentric sleeve 6, the pressing slider 5 is sleeved outside the connecting rod 7 below the eccentric sleeve 6, the pre-tightening spring 4 is sleeved outside the connecting rod 7 between the pressing slider 5 and the upper clamp plate 3, and the upper end and the lower end of the pre-tightening spring 4; at first in driving connecting rod 7 bottom screw in adjusting nut 13 around vertical axle horizontal rotation eccentric bushing 6, reduce the height that compresses tightly slider 5, then rotatory eccentric bushing 6 of rethread rotates around the eccentric shaft, and then compress tightly slider 5 through eccentric biggest radial position, compress tightly slider 5 compression pretension spring 4, the pressure that pretension spring 4 produced drives punch holder 3 and compresses tightly lower plate 1 downwards, elasticity compresses tightly the subassembly like this and ensures reaction piece 2 and lower plate 1, zonulae occludens forms sealed reaction chamber between the detection base plate 9, prevent that reaction liquid from revealing.
As shown in fig. 1, a horizontal flat rectangular space and a cylindrical space are arranged between the reaction block 2 and the lower splint 1, and the cylindrical space is positioned above the rectangular space and is communicated with the rectangular space; the detection substrate 9 is installed in the cuboid space, the detection substrate 9 is a substrate of a PCB board, as shown in fig. 6, the shape and size of the detection substrate 9 are exactly matched with the shape and size of the cuboid space.
Two internal electrodes 11 and two external electrodes 10 are arranged on the upper surface of the detection substrate 9, and the two internal electrodes 11 are respectively and electrically connected with the two external electrodes 10 through respective wires arranged on the surface of the detection substrate 9; the cylindrical space is used as a reaction chamber, and the reaction chamber is a closed space and provides a place for detection reaction. The reaction chamber is filled with reaction liquid, the two internal electrodes 11 are positioned in the reaction chamber, the internal electrodes 11 are directly contacted with the reaction liquid, the two external electrodes 10 are positioned outside the reaction chamber, and the two external electrodes 10 are led out and connected to an external detection instrument to realize the impedance detection function of the reaction liquid; the real-time detection of the internal impedance of the reaction liquid is realized by collecting and measuring the electric signal on the external electrode 10 for further processing and analysis, and the fluorescence end point detection is carried out after the reaction is finished. The external detection instrument may be embodied as an impedance meter.
The inner electrode 11 and the outer electrode 10 together constitute a detection electrode, and the detection electrode, the detection substrate, and an external detection device constitute a detection module.
As shown in FIG. 3, a sample hole 12 for injecting a reaction solution is formed in the center of the top surface of the reaction block 2 above the cylindrical space, the sample hole 12 is sealed with a sealant, the sample hole 12 is used for injecting the reaction solution, and the upper plate 3 is formed with a through hole corresponding to the sample hole 12.
When the experiment is carried out, a sample is injected into the chamber through the sample adding hole 12 above the device matrix and is sealed by using a sealant, so that the loss of reaction liquid and the pollution of aerosol during the reaction period are prevented. The device of the invention is disposable and can be discarded after the detection is finished.
A sealing ring 8 is arranged between the bottom surface of the reaction block 2 at the bottom of the reaction chamber and the detection substrate 9, and the sealing of the reaction chamber is kept through the sealing ring 8 and the sealant of the sample adding hole 12.
As shown in fig. 5, hollow out construction 14 has been seted up to punch holder 3 lateral wall around reaction block 2, and hollow out construction 14 specifically is the fretwork groove that the multichannel link up, can arrange water bath heating in with whole device, arranges hollow out construction 14 around reaction chamber, can reduce device material wall thickness and cause thermal-insulated problem, can increase reaction chamber receive water bath heating area, reduce the heat isolation effect that reaction chamber wall thickness brought, improves heating rate.
Or the entire apparatus may be placed in a radiant environment for radiant heating.
When the experiment is carried out, a proper heating mode, such as water bath heating, radiation heating and the like, is selected according to the experiment requirement. The above is only one of many heating means and any means that can be implemented to heat the device can be applied here.
In a specific embodiment, the detection substrate 9 may be extended out of a surrounding case formed between the reaction block 2 and the lower plate 1, and the two external electrodes 10 may be electrically connected to an external detection device through respective wires after being exposed out of the surrounding case.
The experimental implementation working process of the invention is as follows:
before experimental detection, the upper clamping plate 3 is not installed, reaction liquid of pollutants such as bacteria, fungi and viruses is injected into the reaction chamber through the sample adding hole 12 on the device substrate, and then the sample adding hole 12 is sealed by using sealant, so that liquid loss and aerosol pollution in the reaction process are avoided. Through the design of direct pressure type, the reaction chamber and the detection electrode can be effectively compressed, and liquid leakage is avoided.
The test device is placed in an environment suitable for the growth of bacteria, pollutants such as bacteria, fungi, viruses and the like are propagated in the reaction liquid, and the detection of the number of the bacteria can be realized by detecting the impedance change in the test system. The change in the amount changes the impedance of the reaction system. The impedance signal of the reaction system is accessed to external detection equipment, and the function of monitoring the pollutants in the reaction liquid is realized by monitoring the impedance change of the reaction system in real time in the reaction process.
The device is designed into a quick-release structure, and the spring can be loosened or compressed only by rotating the eccentric sleeve 5 by a certain angle, namely, the device is opened and closed to replace and tighten the reaction chamber, so that quick release is realized. The structure of quick detach type can the new reaction chamber of quick replacement come the going on of carrying out the experiment next step, because the reaction chamber sets for once only, need not carry out extra processing to the reaction chamber, avoids the influence of uncontrollable factor to the experiment. The whole device is small and portable, can quickly realize the detection of the target object in an impedance mode, and is favorable for realizing field detection.
The device is abandoned after the detection is finished, so that aerosol pollution caused by subsequent opening of the reaction container is avoided.
After the experiment is completed, the lower clamping plate 1, the reaction block 2 and the upper clamping plate 3 can be discarded, and the elastic pressing assembly can be reused after being sterilized.
Claims (6)
1. A disposable quick detach type pollutant impedance detection device which characterized in that:
comprises a device base body, an internal electrode (11) and an external electrode (10); the device base body comprises a lower clamping plate (1), a reaction block (2), an upper clamping plate (3) and an elastic pressing assembly, wherein the lower clamping plate (1) and the upper clamping plate (3) are vertically butted and installed into an integral shell, and the reaction block (2) is installed between the lower clamping plate (1) and the upper clamping plate (3); the lower clamping plate (1) and the upper clamping plate (3) are fixedly connected through an elastic pressing assembly, so that the reaction block (2) is tightly clamped between the lower clamping plate (1) and the upper clamping plate (3), the elastic pressing assembly comprises a pre-tightening spring (4), a pressing sliding block (5), an eccentric sleeve (6), a connecting rod (7) and an adjusting nut (13), the bottom of the adjusting nut (13) is fixed in a mounting counter bore formed in the lower clamping plate (1), the bottom end of the connecting rod (7) is screwed into the adjusting nut (13) through a thread, the top end of the connecting rod (7) penetrates through a through hole formed in the upper clamping plate (3) and then is eccentrically hinged with the eccentric sleeve (6), the pressing sliding block (5) is sleeved outside the connecting rod (7) below the eccentric sleeve (6), and the pre-tightening spring (4) is sleeved outside the connecting rod (7) between the pressing sliding block (;
a horizontal flat cuboid space and a cylindrical space are arranged between the reaction block (2) and the lower clamping plate (1), and the cylindrical space is positioned above the cuboid space and is communicated with the cuboid space; a detection substrate (9) is arranged in the cuboid space, two internal electrodes (11) and two external electrodes (10) are arranged on the upper surface of the detection substrate (9), and the two internal electrodes (11) are respectively and electrically connected with the two external electrodes (10) through respective wires arranged on the surface of the detection substrate (9); the cylindrical space is used as a reaction chamber, the reaction chamber is filled with reaction liquid, the two internal electrodes (11) are positioned in the reaction chamber, the internal electrodes (11) are directly contacted with the reaction liquid, the two external electrodes (10) are positioned outside the reaction chamber, and the two external electrodes (10) are led out and connected to an external detection instrument; the center of the top surface of the reaction block (2) above the columnar space is provided with a sample adding hole (12) for injecting reaction liquid, and the sample adding hole (12) is sealed by sealant.
2. A disposable quick disconnect type contaminant impedance sensing device in accordance with claim 1, wherein: and a sealing ring (8) is arranged between the bottom surface of the reaction block (2) at the bottom of the reaction chamber and the detection substrate (9).
3. A disposable quick disconnect type contaminant impedance sensing device in accordance with claim 1, wherein: the shape and the size of the detection substrate (9) are just matched with those of the cuboid space.
4. A disposable quick disconnect type contaminant impedance sensing device in accordance with claim 1, wherein: the side wall of the upper clamping plate (3) around the reaction block (2) is provided with a hollow structure (14), and the hollow structure (14) is a plurality of through hollow grooves.
5. A disposable quick disconnect type contaminant impedance sensing device in accordance with claim 1, wherein: the detection substrate (9) extends out of a surrounding shell formed between the reaction block (2) and the lower clamping plate (1), and the two external electrodes (10) are exposed out of the surrounding shell and then are electrically connected with an external detection instrument through respective wires.
6. A disposable quick disconnect type contaminant impedance sensing device in accordance with claim 1, wherein: the pollutants are liquid pollutants of bacteria, fungi and viruses.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910939672.1A CN110658234B (en) | 2019-09-30 | 2019-09-30 | Disposable quick-dismantling type pollutant impedance detection device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910939672.1A CN110658234B (en) | 2019-09-30 | 2019-09-30 | Disposable quick-dismantling type pollutant impedance detection device |
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| CN110658234A true CN110658234A (en) | 2020-01-07 |
| CN110658234B CN110658234B (en) | 2021-02-26 |
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| CN201910939672.1A Active CN110658234B (en) | 2019-09-30 | 2019-09-30 | Disposable quick-dismantling type pollutant impedance detection device |
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| CN110160759A (en) * | 2019-03-21 | 2019-08-23 | 上海思晋智能科技有限公司 | A kind of novel clamping device of knob test |
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2019
- 2019-09-30 CN CN201910939672.1A patent/CN110658234B/en active Active
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| JP2002350347A (en) * | 2001-05-22 | 2002-12-04 | Matsushita Electric Ind Co Ltd | Fluorescence detecting apparatus |
| CN101430326A (en) * | 2007-11-05 | 2009-05-13 | 百美生化科技股份有限公司 | Biochemical sensor and its production method |
| EP2103933A1 (en) * | 2008-02-25 | 2009-09-23 | KeyNeurotek Pharmaceuticals AG | Device and method for measuring impedance in organotypic tissues |
| CN102539486A (en) * | 2010-12-31 | 2012-07-04 | 香港理工大学 | Chip and bacterium analysis instrument for food safety detection |
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| CN110658234B (en) | 2021-02-26 |
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