CN110655590A - Production method of pachyman - Google Patents
Production method of pachyman Download PDFInfo
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- CN110655590A CN110655590A CN201911004886.6A CN201911004886A CN110655590A CN 110655590 A CN110655590 A CN 110655590A CN 201911004886 A CN201911004886 A CN 201911004886A CN 110655590 A CN110655590 A CN 110655590A
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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Abstract
The invention discloses a production method of pachyman, which comprises the following steps: s1, selecting dried tuckahoe, and crushing the tuckahoe into 175-185-mesh particles; s2, soaking the crushed tuckahoe in water for 3.5 to 4.5 hours to obtain tuckahoe soak solution; s3, adding an NAOH solution into the poria cocos soaking solution, wherein the mass ratio of the poria cocos to the NAOH solution is 1: 7-1: 5, and filtering after dissolution to obtain a poria cocos alkali solution; s4, taking an NAOH solution and a chloroacetic acid solution with the same mass, neutralizing, and adding the neutralized solution and the pachyman alkali solution into a reaction kettle for reaction to obtain a poria cocos comprehensive solution; s5, extracting the poria cocos comprehensive solution with alcohol to obtain a pachyman primary product; performing alcoholization on the pachymaran product to obtain a pachymaran refined product; air drying and pulverizing to obtain pachyman product. The method can effectively produce pachyman finished products, has higher quality, more delicate appearance and higher pachyman content, and is convenient for industrial production.
Description
Technical Field
The invention relates to the field of pachyman production processes, and in particular relates to a production method of pachyman.
Background
Poria has attracted attention because of its high medicinal value, the medicinal part of Poria is Poria sclerotium, and pachyman is the main effective component of Poria. Recent clinical and pharmacological studies show that pachyman has good anti-tumor effect, the saccharide has the effects of enhancing cytotoxicity of macrophages and T lymphocytes, enhancing cellular immune response and activating an immune monitoring system of an organism on tumors, and the mechanism of the saccharide is related to the activation of complement. Poria cocos raw material is Poria cocos monosaccharide, and Poria cocos polysaccharide is obtained through processing.
The pachyman produced in the prior art is low in quality, and is black or brown in appearance, and the finished product of the pachyman is low in quality, contains more impurities and is low in pachyman content. Meanwhile, the production process also causes raw material waste and has low production rate.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a production method of pachyman, which improves the quality of a finished product of the pachyman.
In order to achieve the purpose, the invention provides the following technical scheme:
a production method of pachyman comprises the following steps:
s1, selecting dried tuckahoe, and crushing the tuckahoe into 175-185-mesh particles;
s2, soaking the crushed tuckahoe in water for 3.5 to 4.5 hours to obtain tuckahoe soak solution;
s3, adding an NAOH solution into the poria cocos soaking solution, wherein the mass ratio of the poria cocos to the NAOH solution is 1: 7-1: 5, and filtering after dissolution to obtain a poria cocos alkali solution;
s4, taking an NAOH solution and a chloroacetic acid solution with the same mass, neutralizing, and adding the neutralized solution and the pachyman alkali solution into a reaction kettle for reaction to obtain a poria cocos comprehensive solution;
s5, extracting the poria cocos comprehensive solution with alcohol to obtain a pachyman primary product; performing alcoholization on the pachymaran product to obtain a pachymaran refined product; air drying and pulverizing to obtain pachyman product.
By adopting the scheme, the finished pachymaran product with high quality can be effectively produced.
More preferably: in step S5, the step of extracting with ethanol comprises selecting 95% ethanol, placing in an alcohol tank, slowly adding the Poria complex solution until the ethanol concentration is diluted to 70%, and centrifuging to obtain pachyman primary product.
By adopting the scheme, the pachyman can be extracted from the tuckahoe comprehensive liquid.
More preferably: in step S5, the alcohol refining step includes adding 95% ethanol with the same mass as that of the previous step into an alcohol tank, adding the pachyman product, soaking for 1.5-2.5h, centrifuging, and repeatedly refining with alcohol for two to three times to obtain a pachyman refined product.
By adopting the scheme, impurities in the primary pachymaran can be cleaned, and the crystallinity of pachymaran is enhanced, so that the appearance color of the produced pachymaran product becomes light and white, and the quality of the product is higher.
More preferably: in the step S4, the reaction condition in the reaction kettle is that the reaction kettle is controlled to be continuously heated to 74-76 ℃ for 4 hours, and then the reaction kettle is kept at a constant temperature for 8-10 hours.
By adopting the scheme, the extraction yield of pachyman can be improved.
More preferably: in step S2, the soaking time is 4 hours; in step S3, the mass ratio of the poria cocos to the NAOH solution is 1: 6; the constant temperature of the reaction kettle is 75 ℃.
By adopting the scheme, the quality guarantee of pachyman can be improved.
More preferably: in step S4, the ratio of the neutralized volume of NAOH solution and chloroacetic acid solution to the volume of the pachymatic base solution is 4: 3.
by adopting the scheme, the monosaccharide structure in the poria cocos can be fully destroyed, chains among the monosaccharides are broken, and the pachymaran is recombined.
More preferably: the soaking time in the latter alcohol forming step is longer than that in the former alcohol forming step.
By adopting the scheme, impurities in the primary pachymaran can be better cleaned, and the crystallinity of pachymaran is enhanced.
More preferably: in step S1, the dried poria cocos is in a shape of a dice or a sheet.
By adopting the scheme, the poria cocos is conveniently crushed.
More preferably: in step S5, the pachyman product is 195-205 mesh powder.
Adopt above-mentioned scheme, do benefit to storage and finished product powdered, the outward appearance is more exquisite.
More preferably: in step S4, the concentration of the NAOH solution is 25% and the concentration of the chloroacetic acid solution is 50%.
By adopting the scheme, the monosaccharide structure in the poria cocos is fully and effectively destroyed, chains among the monosaccharides are broken, and the pachymaran is recombined.
In conclusion, the invention has the following beneficial effects: the finished product of pachymaran can be effectively produced, the quality of the finished product is better, the appearance is more delicate, the pachymaran content is higher, and the pachymaran content is about 20 percent higher than that of the finished product of pachymaran produced by the common process; accurate process condition control, guaranteed pachyman production quality, and convenient for batch production.
Drawings
FIG. 1 illustrates steps of a method for producing pachyman in accordance with one embodiment of the present invention;
FIG. 2 shows the steps of a pachyman production process according to one embodiment of the present invention.
Detailed Description
The invention is described in detail below with reference to the figures and examples.
In one embodiment of the present invention, there is provided a method for producing pachyman, referring to fig. 1 to 2, comprising the following steps
S1, selecting 0.5kg of dried tuckahoe, and crushing into 180-mesh particles;
s2, soaking the crushed poria cocos in water for 4 hours to obtain a poria cocos soaking solution;
s3, adding an NAOH solution into the poria cocos soaking solution, wherein the mass of the NAOH solution is 3kg, and filtering after the NAOH solution is dissolved to obtain a poria cocos alkali solution;
s4, taking 2L of NAOH solution and 2L of chloroacetic acid solution, neutralizing, and adding the neutralized solution and the pachyman alkali solution into a reaction kettle for reaction to obtain a poria cocos comprehensive solution;
s5, extracting the poria cocos comprehensive solution with alcohol to obtain a pachyman primary product; performing alcoholization on the pachymaran product to obtain a pachymaran refined product; then air-drying and pulverizing to obtain pachyman finished product A.
In step S1, the poria cocos is pulverized into 175-mesh granules, and 185-mesh granules are satisfactory for the completion of the subsequent soaking. The dried tuckahoe is in a shape of a dice or a sheet, and is convenient to crush.
In step S4, the reaction conditions in the reaction kettle are that the temperature of the reaction kettle is controlled to be continuously raised to 75 ℃ for 4 hours, and then the reaction kettle is kept at a constant temperature for 8 hours, wherein the total volume of the pachymatic alkali solution is about 3L. Thus, the extraction yield of pachyman can be greatly improved, and the quality guarantee of pachyman can be improved. Wherein the concentration of the NAOH solution is 25 percent, and the concentration of the chloroacetic acid solution is 50 percent. Can fully destroy monosaccharide structure in Poria, break chains between monosaccharides, and recombine into pachymaran.
In step S5, the step of extracting with ethanol comprises selecting 95% ethanol, placing in an alcohol tank, slowly adding the Poria complex solution until the ethanol concentration is diluted to 70%, and centrifuging to obtain pachyman primary product. The step of alcohol refining comprises the steps of adding 95% ethanol with the same mass as that of the previous step into an alcohol tank, adding the pachyman product, soaking for 2 hours, centrifuging, and repeatedly carrying out alcohol refining for two to three times to obtain a pachyman fine product. Therefore, impurities in the primary pachymaran product can be cleaned, and the crystallinity of the pachymaran is enhanced, so that the appearance color of the produced pachymaran product becomes light and white, and the quality of the product is higher. Preferably, the soaking time of the second or third alcohol composition is longer than that of the previous alcohol composition, for example, it may be 15min. The storage is facilitated, and the finished product is powdery and has more delicate appearance.
In another embodiment of the present invention, there is provided a method for producing pachyman, comprising the steps of
S1, selecting 0.8kg of dried poria cocos, and crushing into 175-mesh granules;
s2, soaking the crushed poria cocos in water for 4.5 hours to obtain a poria cocos soaking solution;
s3, adding an NAOH solution into the poria cocos soaking solution, wherein the mass of the NAOH solution is 4.8kg, and filtering after the NAOH solution is dissolved to obtain a poria cocos alkali solution;
s4, taking 2.65L of NAOH solution and 2.65L of chloroacetic acid solution, neutralizing, and adding the neutralized solution and the pachyman alkali solution into a reaction kettle for reaction to obtain a poria cocos comprehensive solution; wherein the pachyman solution is about 4L.
S5, extracting the poria cocos comprehensive solution with alcohol to obtain a pachyman primary product; performing alcoholization on the pachymaran product to obtain a pachymaran refined product; then air-drying and pulverizing to obtain pachyman finished product B.
In step S4, the reaction conditions in the reaction kettle are controlled such that the temperature of the reaction kettle is continuously increased to 74 ℃ for 4 hours, and then kept at a constant temperature for 9 hours. In step S5, the soaking time was 2.5h, and the remaining process conditions were the same as in the previous first example.
In another embodiment of the present invention, there is provided a method for producing pachyman, comprising the steps of
S1, selecting 1kg of dried tuckahoe, and crushing the tuckahoe into 185-mesh granules;
s2, soaking the crushed poria cocos in water for 3.5 hours to obtain a poria cocos soaking solution;
s3, adding an NAOH solution into the poria cocos soaking solution, wherein the mass of the NAOH solution is 6kg, and filtering after the NAOH solution is dissolved to obtain a poria cocos alkali solution;
s4, taking 4L of NAOH solution and 4L of chloroacetic acid solution, neutralizing, and adding the neutralized solution and the pachyman alkali solution into a reaction kettle for reaction to obtain a poria cocos comprehensive solution; wherein the pachyman solution is about 6L.
S5, extracting the poria cocos comprehensive solution with alcohol to obtain a pachyman primary product; performing alcoholization on the pachymaran product to obtain a pachymaran refined product; and air-drying and pulverizing to obtain pachyman finished product C.
In step S4, the reaction conditions in the reaction kettle are controlled such that the temperature of the reaction kettle is continuously raised to 76 ℃ for 4 hours, and then kept at a constant temperature for 10 hours. In step S5, the soaking time is 1.5h, and the remaining process conditions are the same as in the previous first embodiment.
Selecting certain mass of commercial pachyman finished products D and E to obtain the average pachyman content.
Comparing pachyman content in pachyman finished product A, B, C:
| poria polysaccharide product | A | B | C | D | E |
| Average pachyman content | 75.1％ | 78.3％ | 73.7％ | 56.2％ | 53.1％ |
Pachyman content refers to the amount of pachyman in the finished product, and the finished product may also contain starch and other substances. The content of common pachyman finished products in the market is about 50-60%, while the content of pachyman in the pachyman finished products obtained by the production method of the invention is about 70-80%, which can be about 20% higher.
The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It should be noted that several improvements and modifications without departing from the principle of the present invention will occur to those skilled in the art, and such improvements and modifications should also be construed as within the scope of the present invention.
Claims (10)
1. A production method of pachyman is characterized by comprising the following steps:
s1, selecting dried tuckahoe, and crushing the tuckahoe into 175-185-mesh particles;
s2, soaking the crushed tuckahoe in water for 3.5 to 4.5 hours to obtain tuckahoe soak solution;
s3, adding an NAOH solution into the poria cocos soaking solution, wherein the mass ratio of the poria cocos to the NAOH solution is 1: 7-1: 5, and filtering after dissolution to obtain a poria cocos alkali solution;
s4, taking an NAOH solution and a chloroacetic acid solution with the same mass, neutralizing, and adding the neutralized solution and the pachyman alkali solution into a reaction kettle for reaction to obtain a poria cocos comprehensive solution;
s5, extracting the poria cocos comprehensive solution with alcohol to obtain a pachyman primary product; performing alcoholization on the pachymaran product to obtain a pachymaran refined product; air drying and pulverizing to obtain pachyman product.
2. The method for producing pachyman according to claim 1, wherein: in step S5, the step of extracting with ethanol comprises selecting 95% ethanol, placing in an alcohol tank, slowly adding the Poria complex solution until the ethanol concentration is diluted to 70%, and centrifuging to obtain pachyman primary product.
3. The method for producing pachyman according to claim 1, wherein: in step S5, the alcohol refining step includes adding 95% ethanol with the same mass as that of the previous step into an alcohol tank, adding the pachyman product, soaking for 1.5-2.5h, centrifuging, and repeatedly refining with alcohol for two to three times to obtain a pachyman refined product.
4. The method for producing pachyman according to claim 1, wherein: in the step S4, the reaction condition in the reaction kettle is that the reaction kettle is controlled to be continuously heated to 74-76 ℃ for 4 hours, and then the reaction kettle is kept at a constant temperature for 8-10 hours.
5. The method for producing pachyman according to claim 4, wherein: in step S2, the soaking time is 4 hours; in step S3, the mass ratio of the poria cocos to the NAOH solution is 1: 6; the constant temperature of the reaction kettle is 75 ℃.
6. The method for producing pachyman according to any one of claims 1 to 5, wherein: in step S4, the ratio of the neutralized volume of NAOH solution and chloroacetic acid solution to the volume of the pachymatic base solution is 4: 3.
7. the method for producing pachyman according to claim 3, wherein: the soaking time in the latter alcohol forming step is longer than that in the former alcohol forming step.
8. The method for producing pachyman according to claim 1, wherein: in step S1, the dried poria cocos is in a shape of a dice or a sheet.
9. The method for producing pachyman according to claim 1, wherein: in step S5, the pachyman product is 195-205 mesh powder.
10. The method for producing pachyman according to claim 1, wherein: in step S4, the concentration of the NAOH solution is 25% and the concentration of the chloroacetic acid solution is 50%.
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Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1554667A (en) * | 2003-12-26 | 2004-12-15 | 三明市真菌研究所 | Process for producing carboxymethyl pachyman |
| CN1762378A (en) * | 2005-10-10 | 2006-04-26 | 戴甲木 | Indian bread polysaccharide formulation and its preparation method |
| CN1970579A (en) * | 2006-11-28 | 2007-05-30 | 戴甲木 | High substitution degree carboxymethyl indianbread polysaccharide and its preparation method and uses |
| CN101343331A (en) * | 2008-08-12 | 2009-01-14 | 王永江 | Synthesis of substituted pachyman |
| CN101392030A (en) * | 2008-07-05 | 2009-03-25 | 陈勇 | Middle substitution degree carboxymethyl pachyman, preparation method thereof and use thereof |
| CN101407555A (en) * | 2007-10-13 | 2009-04-15 | 胡荣希 | Novel industrialized production technique of carboxymethyl pachyman |
| CN101724091A (en) * | 2009-12-15 | 2010-06-09 | 湖北省宏源药业有限公司 | Active carboxymethylpachymaran, production process and application thereof |
| CN102898533A (en) * | 2012-10-08 | 2013-01-30 | 湖北省宏源药业有限公司 | Preparation method of carboxymethyl pachymaran |
| CN105348407A (en) * | 2015-12-04 | 2016-02-24 | 徐晓飞 | Preparation process and application of carboxymethyl pachymaran |
| CN107353355A (en) * | 2017-06-22 | 2017-11-17 | 兰溪市奥而特农业科技有限公司 | The preparation method of high activity carboxymethylpachymaran |
| CN109988247A (en) * | 2017-12-29 | 2019-07-09 | 瑞普（天津）生物药业有限公司 | A kind of new preparation process of carboxymethylpachymaran |
-
2019
- 2019-10-22 CN CN201911004886.6A patent/CN110655590A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1554667A (en) * | 2003-12-26 | 2004-12-15 | 三明市真菌研究所 | Process for producing carboxymethyl pachyman |
| CN1762378A (en) * | 2005-10-10 | 2006-04-26 | 戴甲木 | Indian bread polysaccharide formulation and its preparation method |
| CN1970579A (en) * | 2006-11-28 | 2007-05-30 | 戴甲木 | High substitution degree carboxymethyl indianbread polysaccharide and its preparation method and uses |
| CN101407555A (en) * | 2007-10-13 | 2009-04-15 | 胡荣希 | Novel industrialized production technique of carboxymethyl pachyman |
| CN101392030A (en) * | 2008-07-05 | 2009-03-25 | 陈勇 | Middle substitution degree carboxymethyl pachyman, preparation method thereof and use thereof |
| CN101343331A (en) * | 2008-08-12 | 2009-01-14 | 王永江 | Synthesis of substituted pachyman |
| CN101724091A (en) * | 2009-12-15 | 2010-06-09 | 湖北省宏源药业有限公司 | Active carboxymethylpachymaran, production process and application thereof |
| CN102898533A (en) * | 2012-10-08 | 2013-01-30 | 湖北省宏源药业有限公司 | Preparation method of carboxymethyl pachymaran |
| CN105348407A (en) * | 2015-12-04 | 2016-02-24 | 徐晓飞 | Preparation process and application of carboxymethyl pachymaran |
| CN107353355A (en) * | 2017-06-22 | 2017-11-17 | 兰溪市奥而特农业科技有限公司 | The preparation method of high activity carboxymethylpachymaran |
| CN109988247A (en) * | 2017-12-29 | 2019-07-09 | 瑞普（天津）生物药业有限公司 | A kind of new preparation process of carboxymethylpachymaran |
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