CN110646599A - Device for rapidly extracting hydrogen sulfide in cerebrospinal fluid and serum sample and using method thereof - Google Patents
Device for rapidly extracting hydrogen sulfide in cerebrospinal fluid and serum sample and using method thereof Download PDFInfo
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- CN110646599A CN110646599A CN201910912623.9A CN201910912623A CN110646599A CN 110646599 A CN110646599 A CN 110646599A CN 201910912623 A CN201910912623 A CN 201910912623A CN 110646599 A CN110646599 A CN 110646599A
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- hydrogen sulfide
- cerebrospinal fluid
- sample
- rapidly extracting
- phosphoric acid
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
Abstract
The invention relates to a device for rapidly extracting hydrogen sulfide from cerebrospinal fluid and serum samples and a using method thereof, wherein the device for rapidly extracting hydrogen sulfide from cerebrospinal fluid and serum samples comprises a20 mL closed injector containing a phosphoric acid solution, a centrifugal tube arranged at the needle head of the injector and a micro-sampling needle inserted into a rubber plug of the injector, wherein the centrifugal tube contains a sodium hydroxide solution, and the micro-sampling needle is used for absorbing a sample to be extracted; the method has the characteristics of simple operation, quick treatment, low cost and the like, and can quickly extract the hydrogen sulfide in the cerebrospinal fluid and the serum sample.
Description
Technical Field
The invention relates to a device for rapidly extracting hydrogen sulfide from cerebrospinal fluid and serum samples and a using method thereof.
Background
Hydrogen sulfide (H)2S) is colorless, inflammable and extremely toxic gas with the odor of rotten eggs, and mainly comes from factories, mining industry and petrochemical industry. If the waste water containing hydrogen sulfide is not treated or is discharged beyond the standard, serious damage can be caused to the ecological environment, and even the health of human beings is seriously threatened. Recent studies have demonstrated that hydrogen sulfide is the third gas signaling molecule found following Nitric Oxide (NO) and carbon monoxide (CO), playing an important role in physiological processes such as nerve conduction, inflammation, vasodilation, apoptosis, cell growth regulation, and cardiovascular protection. The physiological level of hydrogen sulfide in mammalian serum is in the range of about 30-100. mu.M, while the level of hydrogen sulfide in brain can be as high as 160. mu.M. In addition, abnormal levels of hydrogen sulfide in vivo have been associated with a number of diseases such as down syndrome, alzheimer's disease, diabetes and cirrhosis of the liver. However, the cerebrospinal fluid and the serum contain a large amount of interferents which can affect the detection of the hydrogen sulfide, so that the selective extraction of the hydrogen sulfide in the serum and the brain tissue can avoid the influence of interfering species during detection, and the method has important significance for deeply understanding the physiological function of the serum and even for early diagnosis of diseases and cancers.
Disclosure of Invention
Aiming at a series of problems of complicated pretreatment, long time consumption, complex equipment operation, high cost and the like in the prior detection of hydrogen sulfide in complex environments of serum and cerebrospinal fluid, the invention provides a device for rapidly extracting the content of hydrogen sulfide in cerebrospinal fluid and serum samples and a using method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention relates to a device for rapidly extracting hydrogen sulfide from cerebrospinal fluid and serum samples, which comprises a closed syringe containing 20 mL, a centrifuge tube arranged at the needle head of the syringe and a micro sample injection needle inserted into the rubber plug of the syringe; the closed injector consists of a syringe A, a rubber plug A and a push rod A; the micro sample injection needle consists of a needle cylinder B, a rubber plug B and a push rod B.
Further, the above closed syringe was filled with 5 mL of a 1 mol/L phosphoric acid solution.
Furthermore, the micro sample injection needle sucks 100 mu L of sample to be extracted, and a rubber plug is inserted into the lower end of the injector; the sample to be extracted is cerebrospinal fluid or serum sample.
Further, 1 mL of 0.1M NaOH solution was added to the tube.
The use method of the device comprises the following steps:
1) adding 5 mL of 1 mol/L phosphoric acid solution into a20 mL closed syringe;
2) a micro sample injection needle sucks 100 mu L of sample to be extracted, and a rubber plug is inserted into the lower end of the injector;
3) inserting the needle head of the syringe needle into a centrifugal tube;
4) 1 mL of 0.1M NaOH solution is added into the centrifugal tube;
5) and slowly pushing the sample in the micro sample injection needle into the injector to enable the sample to fully react with the phosphoric acid, slowly pushing the piston of the injector upwards until the upper liquid level of the phosphoric acid solution is contacted with the top in the injector without air when the phosphoric acid solution does not generate bubbles any more, and collecting the reacted sodium hydroxide solution in the centrifugal tube by using a pipette.
The device for rapidly extracting the hydrogen sulfide content in the cerebrospinal fluid and the serum sample is constructed by utilizing the simple injector and the sample injection needle, has low cost and convenient operation, and is suitable for rapidly extracting the hydrogen sulfide in the cerebrospinal fluid and the serum sample on site so as to be used for next detection.
Description of the drawings:
FIG. 1 is a schematic diagram of the apparatus of the present invention;
FIG. 2 is a schematic view of a closed syringe structure of the apparatus of the present invention;
FIG. 3 is a schematic view of a micro-injection needle structure in the device of the present invention;
FIG. 4 is a top view of the plunger A of the encapsulated syringe of the device of the present invention;
FIG. 5 is a front view of the plunger A of the obturator injector of the device of the present invention;
in the figure: 1-centrifuge tube, 2-closed injector, 201-syringe A, 202-rubber plug A, 203-push rod A, 3-micro sample injection needle, 301-syringe B, 302-rubber plug B, 303-push rod B.
The specific implementation mode is as follows:
in order to make the present invention more comprehensible, the technical solutions of the present invention are further described below with reference to specific embodiments, but the present invention is not limited thereto.
Examples
As shown in figure 1, the device for rapidly extracting hydrogen sulfide content in cerebrospinal fluid and serum samples comprises a centrifuge tube 1 for containing sodium hydroxide solution, a20 mL closed syringe 2 for containing phosphoric acid solution and a micro-sampling needle 3 for sucking samples to be extracted, wherein the closed syringe consists of a needle cylinder A, a rubber plug A and a push rod A; the micro sample injection needle consists of a needle cylinder B, a rubber plug B and a push rod B. .
In the embodiment, for reasonable and good test, the water used in the test is ultrapure water; ultrapure water, also known as UP water, has a resistivity of 18M Ω cm (25 ℃).
In this example, the concentration of the phosphoric acid solution was 1 mol/L, and the volume was 5 mL; the concentration of the sodium hydroxide solution is 0.1M, and the volume is 1 mL; the sample to be extracted was a serum sample with a volume of 100. mu.L.
The using method comprises the following steps: 5 mL of 1 mol/L phosphoric acid solution is added into the closed injector 2; the micro sample injection needle 3 sucks 100 mu L of serum sample, and then a rubber plug A202 is inserted from the lower end of the closed injector 2; 1 mL of 0.1M NaOH solution is added into a centrifuge tube 1, then the centrifuge tube 1 is inserted into a needle head of a needle cylinder A201 in a closed syringe 2, then a serum sample in a micro-injection needle 3 is slowly pushed into the closed syringe 2 to be fully reacted with phosphoric acid, when the phosphoric acid solution does not generate bubbles any more, a piston of the closed syringe 2 is slowly pushed upwards to the upper liquid level of the phosphoric acid solution to be in contact with the top of the closed syringe 2 without air, a pipette is used for collecting the reacted sodium hydroxide solution in the centrifuge tube 1, and then the extraction of hydrogen sulfide in the serum sample is quickly completed.
The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.
Claims (9)
1. The utility model provides a draw device of hydrogen sulfide in cerebrospinal fluid and serum sample fast which characterized in that: the device comprises a closed syringe, a centrifugal tube inserted at the needle head of the closed syringe and a micro-sampling needle inserted at the rubber plug of the closed syringe, wherein the centrifugal tube is filled with a sodium hydroxide solution, and a sample to be extracted is absorbed in the micro-sampling needle.
2. The device for rapidly extracting hydrogen sulfide from cerebrospinal fluid and serum samples as claimed in claim 1, wherein: the closed injector consists of a syringe A, a rubber plug A and a push rod A.
3. The device for rapidly extracting hydrogen sulfide from cerebrospinal fluid and serum samples as claimed in claim 1, wherein: the micro sample injection needle consists of a needle cylinder B, a rubber plug B and a push rod B.
4. The device for rapidly extracting hydrogen sulfide from cerebrospinal fluid and serum samples as claimed in claim 1, wherein: the closed syringe is filled with phosphoric acid solution, and the volume of the closed syringe is 20 mL.
5. The device for rapidly extracting hydrogen sulfide from cerebrospinal fluid and serum samples as claimed in claim 4, wherein: the concentration of the phosphoric acid solution is 1 mol/L, and the volume is 5 mL.
6. The device for rapidly extracting hydrogen sulfide from cerebrospinal fluid and serum samples as claimed in claim 1, wherein: the concentration of the sodium hydroxide solution is 0.1M, and the volume is 1 mL.
7. The device for rapidly extracting hydrogen sulfide from cerebrospinal fluid and serum samples as claimed in claim 1, wherein: the sample to be extracted is cerebrospinal fluid or serum sample, and the volume is 100 mu L.
8. A method for using the device for rapidly extracting hydrogen sulfide from cerebrospinal fluid and serum samples as claimed in any one of claims 1 to 7, characterized in that: the method comprises the following steps:
1) adding 5 mL of 1 mol/L phosphoric acid solution into a20 mL closed syringe;
2) a micro sample injection needle sucks 100 mu L of sample to be extracted, and a rubber plug is inserted into the lower end of the injector;
3) inserting the needle head of the syringe needle into a centrifugal tube;
4) 1 mL of 0.1M NaOH solution is added into the centrifugal tube;
5) and slowly pushing the sample to be extracted in the micro sample injection needle into the injector to enable the sample to be fully reacted with the phosphoric acid, slowly pushing the piston of the injector upwards until the upper liquid level of the phosphoric acid solution is contacted with the top in the injector without air when the phosphoric acid solution does not generate bubbles any more, and collecting the reacted sodium hydroxide solution in the centrifugal tube by using a pipette.
9. The use method of the device for rapidly extracting hydrogen sulfide from cerebrospinal fluid and serum samples as claimed in claim 8, wherein: the time for the sample to be extracted to fully react with the phosphoric acid is 5-8 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201910912623.9A CN110646599B (en) | 2019-09-25 | 2019-09-25 | Device for rapidly extracting hydrogen sulfide in cerebrospinal fluid and serum sample and using method thereof |
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| CN201910912623.9A CN110646599B (en) | 2019-09-25 | 2019-09-25 | Device for rapidly extracting hydrogen sulfide in cerebrospinal fluid and serum sample and using method thereof |
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| CN110646599B CN110646599B (en) | 2021-03-30 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115541861A (en) * | 2022-12-05 | 2022-12-30 | 山东第一医科大学附属省立医院(山东省立医院) | Body fluid sampling and detecting device for neurology department |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115541861A (en) * | 2022-12-05 | 2022-12-30 | 山东第一医科大学附属省立医院(山东省立医院) | Body fluid sampling and detecting device for neurology department |
| CN115541861B (en) * | 2022-12-05 | 2023-03-10 | 山东第一医科大学附属省立医院(山东省立医院) | Body fluid sampling and detecting device for neurology department |
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| CN110646599B (en) | 2021-03-30 |
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