CN110646387B - Kit for flow-type fluorescence detection method - Google Patents
Kit for flow-type fluorescence detection method Download PDFInfo
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- CN110646387B CN110646387B CN201910875954.XA CN201910875954A CN110646387B CN 110646387 B CN110646387 B CN 110646387B CN 201910875954 A CN201910875954 A CN 201910875954A CN 110646387 B CN110646387 B CN 110646387B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/52—Containers specially adapted for storing or dispensing a reagent
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/06—Test-tube stands; Test-tube holders
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
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Abstract
A kit for a flow-type fluorescence detection method comprises a kit body, a detection device and a control device, wherein the kit body consists of a liquid storage part, a pipe frame and an illumination plate, and the illumination plate is arranged between the liquid storage part and the pipe frame part or at the bottom; a plurality of partition plates are vertically arranged in the liquid storage part to divide the liquid storage part into a plurality of liquid storage chambers; the lower part of one side of the liquid storage chamber, which is far away from the pipe frame part, is provided with an L-shaped liquid outlet pipe which comprises a horizontal part and an upward bending part; the top end of the bending part is provided with an opening and a cover; a short pipe is horizontally fixed at the front end of the cover; the connecting rod penetrates through each short pipe to connect all the covers in series; the liquid storage part is internally provided with a plurality of independent liquid storage chambers, so that the material is saved, and the collision is eliminated; the liquid storage part, the pipe frame part and the lighting plate form a stable whole, so that the tube frame part is convenient to take and use, and a test tube and the like can be placed on the pipe frame part; a plurality of lids connected in series by the connecting rod can be opened simultaneously at one time, so that the trouble of opening the lids for multiple times respectively is avoided, the detection efficiency is improved, and the rapid detection operation is facilitated.
Description
Technical Field
The invention belongs to the technical field of kits, and particularly relates to a kit for a flow-type fluorescence detection method.
Background
The flow-type fluorescence detection kit is usually used in hospitals, pharmaceutical enterprises, medical and scientific research units and third-party detection departments, and can enable detection personnel to get rid of the heavy reagent preparation and optimization process, thereby greatly improving the detection efficiency.
The kit generally comprises a plurality of reagents, and in order to achieve the purposes of shock absorption, collision prevention, crushing and the like, a buffering device such as a foam surface and the like is required to be added, but the kit is often single in function; the small bottles for containing the reagent are numerous and complicated, and operators need to open different reagent bottles for multiple times when carrying out pretreatment on the machine, so that the working efficiency is reduced, and the probability of cross contamination, reagent bottle taking by mistake and reagent adding by mistake is increased.
When the pipette is used for operations such as liquid adding, dilution and the like, if the liquid level in the reagent bottle is low, an operator is usually required to pick up the reagent bottle and collect liquid by using the pipette while observing the reagent bottle so as to ensure that the phenomena of empty suction and less reagent suction are avoided and the test accuracy is improved; this requires careful handling and observation by the operator, which reduces the efficiency of the inspection to some extent and increases the workload of the operator.
In operations such as add reagent, filtration, heavy suspension, incubation, the operator generally need observe the circumstances such as the sediment of in vitro liquid, turbidity, and the illumination intensity in the laboratory is insufficient, and direct observation is difficult for playing effectual observation effect, needs to take up the test tube to observe the light source occasionally.
In addition, a plurality of reagent bottles need separately pack, have increased the packaging cost to a certain extent, cause the waste of certain degree, are unfavorable for the environmental protection.
In view of the above, there is a need for a reagent kit that provides illumination, avoids multiple decaps, and facilitates collection of liquid by a pipette.
Disclosure of Invention
The invention aims to provide a kit for a flow-type fluorescence detection method, which can quickly finish pretreatment operations such as liquid preparation, dilution and the like.
It is another object of the present invention to provide a kit that reduces fluid extraction errors, provides supplemental lighting, and reduces operator effort.
In order to solve the technical problem, the invention discloses a kit for a flow-type fluorescence detection method, which comprises a kit body, wherein the kit body comprises: the device comprises a liquid storage part, a pipe frame part and an illumination plate, wherein the liquid storage part is used for storing different reagents, the pipe frame part is used for placing test tubes, the liquid storage part and the pipe frame part are arranged adjacently, and the illumination plate is arranged between the liquid storage part and the pipe frame part or at the bottom;
a plurality of partition plates are vertically arranged in the liquid storage part, and the partition plates divide the inner cavity of the liquid storage part into a plurality of independent liquid storage chambers; the lower part of one side of each liquid storage chamber, which is far away from the pipe frame part, is provided with an L-shaped liquid outlet pipe, the liquid outlet pipe is communicated with the liquid storage chambers, and the liquid outlet pipe comprises a horizontal part and an upward bent part;
an opening is formed in the top end of the bent part, and a cover is arranged on the opening;
a short pipe is horizontally fixed at the front end of each cover;
the connecting rod penetrates through each short pipe to connect all the covers in series, so that all the covers can be opened at one time.
Preferably, one side of the cover, which is far away from the liquid storage chamber, is connected with the top end of the bending part, and the cover is opened in a manner that the cover is turned over from one side, which is near the liquid storage chamber, to one side, which is far away from the liquid storage chamber.
Preferably, the length of the bending part higher than the horizontal part is not less than 1cm, an extension part is arranged at the bottom of the bending part downwards, and the bottom of the inner side of the extension part is lower than the bottom of the liquid storage chamber.
Preferably, the bottom of the inner side of the extension part is in a shape of a cone or a cambered surface which is concave downwards.
Preferably, the top end opening of the bending part is in a horn shape.
Preferably, the connecting rod is a hard rod, and the connecting rod is detachable.
Preferably, the width of each of the reservoirs is set differently according to the volume required for each of the reservoirs.
Preferably, when the lighting plate is arranged at the bottom of the liquid storage part and the pipe frame part, the thickness of the lighting plate is the same as or close to the height of the extension part.
Preferably, the bottom of the extension part and the pipe frame part are provided with anti-skid pads.
Preferably, the lighting panel is a thin light source panel with a storage battery and uniform and soft light emission.
The kit for the flow-type fluorescence detection method has at least the following advantages:
1. establish the illumination board between stock solution portion and pipe support portion or bottom, conveniently observe the interior liquid level of test tube interior liquid and stock solution room to and provide auxiliary lighting to detection operation.
2. Be equipped with a plurality of independent stock solution rooms in the stock solution portion, so not only save material, eliminated the broken problem of different reagent bottle collisions to, the range of stock solution room is fixed in the kit of same product type, and convenient observation is more difficult to make mistakes after the operator is familiar with the reagent range of stock solution room, and efficiency is higher.
3. Stock solution portion, pipe frame portion and illumination board form the firm whole of structure, and convenient the taking up simultaneously or put down, and pipe frame portion can be used to place the test tube etc. need not to dispose the test-tube rack in addition again, and it is little to occupy the place, convenient and fast.
4. The plurality of covers connected in series by the connecting rod can be opened at the same time, so that the trouble of opening the covers for multiple times respectively is avoided, the detection efficiency is improved, and the quick detection operation is facilitated; if all the covers do not need to be opened at one time, the connecting rods are removed, the covers can be opened respectively, and the short pipe can be used as a handle of the covers, so that the opening is very convenient.
The liquid outlet pipe of L shape makes opening and stock solution portion outer wall keep certain distance, touches stock solution portion outer wall when preventing to move liquid rifle to gather liquid, avoids polluting.
6. Adopt the linker principle, the stock solution room keeps the intercommunication with the kink, and liquid passes through in the horizontal part gets into the kink in the stock solution room to keep stable liquid level in the kink, thereby make skilled operator get in the liquid operation need not to observe the liquid level often and can effectively get liquid, reduced and got the liquid error, and, stable liquid level can also prevent to spill when the lid is opened and leak.
7. There is liquid to drip sometimes during the liquid-transfering gun operation, because all lids are the open mode, the liquid that drips can cause cross contamination if get into other openings, make the open mode of lid turn over for keeping away from liquid storage chamber one side by nearly liquid storage chamber side direction, move forward slightly after the liquid-transfering gun gets liquid, add the test tube after the vertical migration in the space of horizontal part top in, so can effectually avoid the liquid of dripping to fall into opening or lid inner wall on, effectively avoid cross contamination.
8. The extension part is arranged to enable liquid in the liquid storage chamber to flow out completely, and the recess at the bottom of the extension part is beneficial to sucking the liquid, so that waste of expensive reagents is avoided.
Drawings
FIG. 1 is a schematic side view of a kit for use in a flow-type fluorescence detection method. Wherein the direction indicated by the arrow is the lid opening direction.
Fig. 2 is a schematic top view of the kit for the flow-type fluorescence detection method in fig. 1.
FIG. 3 is a schematic diagram of another kit for flow-type fluorescence detection.
The reference signs are: 1-liquid storage part, 2-tube frame part, 3-lighting plate, 4-partition plate, 5-liquid storage chamber, 6-liquid outlet tube, 610-horizontal part, 620-bending part, 630-opening, 640-extending part, 650-extending part inner side bottom, 7-cover, 8-short tube, 9-connecting rod, 10-non-slip pad and A-test tube.
Detailed Description
The present invention is further described in detail below with reference to examples so that those skilled in the art can practice the invention with reference to the description.
It will be understood that terms such as "having," "including," and "comprising," when used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
Examples
As shown in fig. 1-2, a kit for a flow-type fluorescence detection method includes a kit body including: the device comprises a liquid storage part 1 for storing different reagents, a pipe frame part 2 for placing test tubes, and an illumination plate 3, wherein the liquid storage part and the pipe frame part are adjacently arranged, and the illumination plate is arranged between the liquid storage part and the pipe frame part; the liquid storage part, the pipe frame part and the lighting plate are mutually connected and fixed, and in addition, if the lighting plate needs to be recycled or reused, the lighting plate can adopt a detachable design structure, and a switch is arranged on the lighting plate.
A plurality of partition plates 4 are vertically arranged in the liquid storage part, and the partition plates divide the inner cavity of the liquid storage part into a plurality of independent liquid storage chambers 5; an L-shaped liquid outlet pipe 6 is arranged at the lower part of one side of each liquid storage chamber, which is far away from the pipe frame part, the liquid outlet pipes are communicated with the liquid storage chambers, and each liquid outlet pipe comprises a horizontal part 610 and an upward bending part 620; the liquid storage body is stored in the bending part and the extending part, when the liquid level in the bending part is lower than the upper edge of the inner wall of the horizontal part, liquid flows into the bending part from the liquid storage chamber, and stops flowing out until the liquid level in the bending part is not lower than the upper edge of the inner wall of the horizontal part, so that when the liquid in the liquid storage chamber is sufficient, the stable liquid level in the bending part can be always kept, which is very convenient for skilled operators and is beneficial to improving the efficiency. Generally, the liquid level in the bent portion should be kept at a height of not less than 1cm from the upper edge of the opening, i.e., the height of the upward portion of the bent portion is not less than 1cm, so as to prevent the leakage of the reagent due to the vibration generated when the cover is opened. In actual production, labels with different colors or shapes can be pasted on the upper portion of the outer side wall of each liquid storage chamber to facilitate identification of operators, and the liquid storage portion can be made of transparent or semitransparent plastics.
An opening 630 is formed in the top end of the bent part, and a cover 7 is arranged on the opening; in this embodiment, the cover is provided with a hemispherical protrusion for sealing.
A short pipe 8 is horizontally fixed at the front end of each cover.
And a connecting rod 9 which penetrates through each short pipe to connect all the covers in series so that all the covers can be opened at one time. A plurality of reagents are usually needed for one-time detection, the cover is opened one by one only very trouble, and the cover is opened at one time, so that the operation is greatly facilitated, and the cross contamination probability caused by the cover opening hand of an operator is reduced.
One side of the cover, which is far away from the liquid storage chamber, is connected with the top end of the bending part, and the cover is opened in a mode that the cover is turned over from one side, which is near the liquid storage chamber, to one side, which is far away from the liquid storage chamber. Liquid sometimes drips when the pipette is operated, so that the dripped liquid can be prevented from falling into the opening or the inner wall of the cover, and cross contamination is effectively avoided.
The length of the bending part higher than the horizontal part is not less than 1cm, the bottom of the bending part is downwards provided with an extension part 640, and the bottom of the inner side of the extension part is lower than the bottom of the liquid storage chamber.
The bottom 650 of the inner side of the extension part is a downward concave cone or arc surface. Is favorable for reducing the residual liquid when the reagent is used up.
The top end opening of the bending part is in a horn shape. The insertion of the pipette head of the pipette is convenient.
The connecting rod is a hard rod, and the connecting rod can be detached. If all the covers do not need to be opened at one time, the connecting rods are removed, the covers can be opened respectively, and the short pipe can be used as a handle of the covers, so that the opening is very convenient.
The width of each liquid storage chamber is set differently according to the required volume. The waste of volume space is avoided.
The bottom of the extension and the pipe frame part is provided with a non-slip mat 10.
The illumination plate is a thin light source plate which emits light uniformly and softly and is provided with a storage battery. So that the operator can observe the liquid crystal display screen without glaring.
Example 2
As shown in fig. 3, similarly to the embodiment 1, the difference is that the illumination plate is provided at the bottom of the liquid storage part and the pipe frame part, and the thickness of the illumination plate is close to the height of the extension part.
The illumination plate set up the position difference, and the illuminating effect who brings is also different, generally speaking, locates and more is favorable to the observation of test tube A interior liquid between stock solution portion and the pipe support portion, and locates stock solution portion and pipe support portion bottom and then more is favorable to the observation operation that the pipetting gun is more accurate.
The kit can also be used for kits of other detection methods and can be matched with paper outer packages for use.
While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable to various fields of endeavor for which the invention may be embodied with additional modifications as would be readily apparent to those skilled in the art, and the invention is therefore not limited to the details given herein and to the embodiments shown and described without departing from the generic concept as defined by the claims and their equivalents.
Claims (8)
1. A kit for use in a flow-through fluorescence detection method, comprising a kit body, wherein the kit body comprises: the device comprises a liquid storage part, a pipe frame part and an illumination plate, wherein the liquid storage part is used for storing different reagents, the pipe frame part is used for placing test tubes, the liquid storage part and the pipe frame part are arranged adjacently, and the illumination plate is arranged between the liquid storage part and the pipe frame part or at the bottom;
a plurality of partition plates are vertically arranged in the liquid storage part, and the partition plates divide the inner cavity of the liquid storage part into a plurality of independent liquid storage chambers; the lower part of one side of each liquid storage chamber, which is far away from the pipe frame part, is provided with an L-shaped liquid outlet pipe, the liquid outlet pipe is communicated with the liquid storage chambers, and the liquid outlet pipe comprises a horizontal part and an upward bent part;
an opening is formed in the top end of the bent part, and a cover is arranged on the opening;
a short pipe is horizontally fixed at the front end of each cover;
the connecting rod penetrates through each short pipe to connect all the covers in series, so that all the covers can be opened at one time; the connecting rod is detachable;
the length of the bent part higher than the horizontal part is not less than 1cm, an extension part is arranged at the bottom of the bent part downwards, and the bottom of the inner side of the extension part is lower than the bottom of the liquid storage chamber;
the bottom of the inner side of the extension part is a downward-concave conical or cambered surface;
the liquid storage body is stored in the bending part and the extending part, when the liquid level in the bending part is lower than the upper edge of the inner wall of the horizontal part, liquid flows into the bending part from the liquid storage chamber, and stops flowing out until the liquid level in the bending part is not lower than the upper edge of the inner wall of the horizontal part, so that when the liquid in the liquid storage chamber is sufficient, the stable liquid level in the bending part can be always kept.
2. The kit for flow-type fluorescence detection according to claim 1, wherein the side of the lid away from the reservoir is connected to the top end of the bending portion, and the lid is opened by being folded from the side near the reservoir to the side away from the reservoir.
3. The kit for flow-type fluorescence detection according to claim 2, wherein the top opening of the bent portion is trumpet-shaped.
4. The kit for flow-type fluorescence detection according to claim 1, wherein the connecting rod is a rigid rod.
5. The kit for flow-type fluorescence detection according to claim 1, wherein the width of each reservoir is set differently according to the volume required for each reservoir.
6. The reagent kit for flow-type fluorescence detection according to claim 1, wherein the thickness of the illumination plate is the same as or close to the height of the extension portion when the illumination plate is provided at the bottom of the reservoir portion and the tube frame portion.
7. The kit for flow-type fluorescence detection according to claim 6, wherein the extension portion and the bottom portion of the tube frame portion are provided with anti-slip pads.
8. The kit for flow-type fluorescence detection according to any one of claims 1 to 7, wherein the illumination plate is a thin light source plate with a storage battery and uniform and soft light emission.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910875954.XA CN110646387B (en) | 2019-09-17 | 2019-09-17 | Kit for flow-type fluorescence detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910875954.XA CN110646387B (en) | 2019-09-17 | 2019-09-17 | Kit for flow-type fluorescence detection method |
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| CN110646387A CN110646387A (en) | 2020-01-03 |
| CN110646387B true CN110646387B (en) | 2022-04-19 |
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