CN206476989U - A kind of B races streptococcus detection kit - Google Patents
A kind of B races streptococcus detection kit Download PDFInfo
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- CN206476989U CN206476989U CN201621435072.XU CN201621435072U CN206476989U CN 206476989 U CN206476989 U CN 206476989U CN 201621435072 U CN201621435072 U CN 201621435072U CN 206476989 U CN206476989 U CN 206476989U
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Abstract
The utility model discloses a kind of B races streptococcus detection kit, including box body and lid, buffer body is placed with box body, buffer body is provided with multiple shrinkage pools matched with detection reagent bottle diameter, detection reagent bottle is placed with shrinkage pool, detection reagent bottle includes primer kit bottle, probe reagent bottle, t7 rna polymerase reagent bottle, Reverse Transcriptase Reagents bottle, positive control bottle, negative control bottle, Tris HCl reagent bottles, Taq enzyme reagent bottle, mixed reaction solution reagent bottle and cell pyrolysis liquid reagent bottle, box body of the brown color polypropylene material of lucifuge by being made up, polyurethane thermal insulation board is provided with box body, polyurethane thermal insulation board is located at the bottom of box body, on four the week side of boss walls of box body and lid inner side.B races streptococcus detection kit energy described in the utility model is moistureproof, lucifuge, and kit storage occupies little space, convenient transportation;Keep low temperature environment and energy maximum possible is close to permanent low-temperature condition, be conducive to meeting movement requirement, it is ensured that the kit reaches optimum detection effect.
Description
Technical field
The utility model is related to a kind of detection kit, especially a kind of B races streptococcus RNA detection kits.
Background technology
B races streptococcus (Group B Streptococcus, abbreviation GBS) is a kind of conditioned pathogen, in perinatal infection
In ranked first the pathogenic bacteria of position, perinatal infants dead rate can be caused.It is also the important pathogenic bacteria of pregnant and lying-in women's RTI simultaneously, can be with
Cause the complication such as urinary system infection contamination, pregnant and lying-in women's septicemia.When bacterium develops into urogenital tract, and often and monilial infection
Coexist.Pregnant woman's vagina bacterial bearing rate be 5~30%, pregnant woman's bacterial bearing rate with ethnic group, area, the age and it is different, in some special populations,
As bacterial bearing rate in obesity, IGT, multiple pregnancies and the active women of sexual life is higher.Pregnant Women of Perinatal Period B races hammer
The positive substantially increase intrauterine infection of bacterium infection has undesirable effect to pregnancy outcome:Premature rupture of fetal membranes (incidence of disease is 35%), amnion
Chamber infection (incidence of disease is 21%), late abortion, premature labor, puerperal infection etc..B races streptococcal infection simultaneously can also cause neonate
The generation of invasive infection.B races streptococcus can cause sepsis of the newborn, pneumonia, meningitis, or even dead.Deposit after infection
Neonate living, it is also possible to have serious nervous system sequelae, including hydrocephalus, dysnoesia, microcephalus, deafness
Deng.Meanwhile, B races streptococcus can also cause infection of pregnant women, cause premature labor, development of fetus bad (infant of low-birth weight), premature rupture of fetal membranes and
Late abortion.Therefore, detection streptococcic to pregnant woman B races is extremely important and necessary.
At present, the streptococcic detection of B races mainly has following three kinds of methods:(1) traditional Bacteria Culture detection method, this method
Testing result it is more accurate, but detection time is longer, typically needs that result could be come out within 24-76 hours, less efficient, it is impossible to meet
In clinic;(2) although PCR detection methods, detection B races streptococcus DNA, detection method has higher sensitivity, still, detection
When be easily caused intersection amplified reaction or pollution, so as to false positive results occur, so, the application of this method is also by very
Big limitation;(3) collaurum detection method, this method is simple to operate there is provided qualitative detection result, but sensitivity is low, particularly
If infective dose is few, it is possible that missing inspection, the situation of flase drop.
In biological technical field, kit is the important container for taking up reagent bottle.But, existing kit, example
Such as RT-PCR diagnostic kits are usually and are made up of papery, and outside, which is coated, places padded coaming inside one layer of waterproof membrane, kit,
And kit has volume greatly, light-shading effect is poor, takes the cumbersome shortcoming of reagent.
Utility model content
Based on the deficiencies in the prior art, the purpose of this utility model is to provide a kind of protection against the tide, lucifuge, keeps low temperature ring
Border, maximum possible are close to temperature constant state, the B races streptococcus detection kit for being conducive to meeting movement requirement.
To achieve the above object, the technical scheme that the utility model is taken is:A kind of B races streptococcus detection kit, bag
Box body and lid are included, buffer body is placed with the box body, the buffer body is provided with multiple with detection reagent bottle diameter phase
Detection reagent bottle is placed with the shrinkage pool of matching, the shrinkage pool, the detection reagent bottle includes primer kit bottle, probe reagent
Bottle, t7 rna polymerase reagent bottle, Reverse Transcriptase Reagents bottle, positive control bottle, negative control bottle, Tris-HCl reagent bottles, Taq
Enzymatic reagent bottle, mixed reaction solution reagent bottle and cell pyrolysis liquid reagent bottle.
As the improvement of above-mentioned technical proposal, the box body is the box body being made up of the brown color polypropylene material of lucifuge.
It is kept in dark place because biological reagent is generally required, but if directly from the reagent bottle storing reagent of brown color, in life
The state of interior reagent is observed during production or using preceding inconvenient people by reagent bottle, it is impossible to intuitively sentence by naked eyes
Whether with disconnected reagent if for example there are quality problems in muddy or other problemses.And box body of the present invention is used after lucifuge material, reagent
Bottle can select transparent vial body, so can be easy to observe the state of reagent again while reagent is kept in dark place.
As the improvement of above-mentioned technical proposal, polyurethane thermal insulation board is provided with the box body, the polyurethane thermal insulation board is set
In on the bottom of the box body, four the week side of boss walls of box body and the lid inner side.
It is the placement primer kit bottle, probe reagent bottle, polymerase reagent bottle, inverse as the improvement of above-mentioned technical proposal
Transcriptase reagent bottle, the inner side of the shrinkage pool of positive control bottle are provided with polyurethane thermal insulation layer.
As the improvement of above-mentioned technical proposal, ice bag is additionally provided with the box body.
As the improvement of above-mentioned technical proposal, the box body is divided into two rest areas, one of rest area by dividing plate
Cell pyrolysis liquid reagent bottle is placed with, another, which places two row's parallel exhausts of differentiation, other detection reagents bottle, wherein a discharge
It is equipped with primer kit bottle, probe reagent bottle, polymerase reagent bottle, Reverse Transcriptase Reagents bottle, positive control bottle;Another discharge is equipped with
Tris-HCl reagent bottles, Taq enzyme reagent bottle, mixed reaction solution reagent bottle, negative control bottle.
Polyurethane thermal insulation board can play a part of thermal insulation protection, by using cooperatively for polyurethane adiabatic dividing plate and ice bag,
Ensure primer kit bottle, probe reagent bottle, polymerase reagent bottle, Reverse Transcriptase Reagents bottle, the storage temperature of positive control bottle be-
20℃;Ensure that Tris-HCl reagent bottles, Taq enzyme reagent bottle, mixed reaction solution reagent bottle, the storage temperature of negative control bottle are 4
DEG C, so as to ensure that each reagent can keep low temperature environment.
As the improvement of above-mentioned technical proposal, the shrinkage pool top is tapered into cylinder, its underpart diameter.It is so recessed
Hole is combined closely with reagent bottle, and reagent bottle is difficult to skid off in transit.
As the improvement of above-mentioned technical proposal, sponge is filled with the lid, the top structure of the detection reagent bottle is embedding
In sponge, the bottom of the detection reagent bottle is embedded in buffer body.
As the improvement of above-mentioned technical proposal, the buffer body is foam-rubber cushion.
As the improvement of above-mentioned technical proposal, detection reagent bottle is transparence, and the bottle of detection reagent bottle
Wall is provided with scale.So as to be visually known the remaining number of reagent bottle, exactly taken amount be used for test.
The beneficial effects of the utility model are:The utility model provides a kind of B races streptococcus detection kit, the examination
Agent box has advantages below:
(1) the moistureproof lucifuge of kit energy, and kit small volume, storage occupies little space, convenient transportation;
(2) low temperature environment is kept, and maximum possible is close to temperature constant state, is conducive to meeting the requirements in transit, it is ensured that should
Kit reaches optimum detection effect;
(3) B races streptococcus detection kit of the present utility model is by setting thermal insulation layer and ice bag, it is possible to provide primer kit
Bottle, probe reagent bottle, t7 rna polymerase reagent bottle, Reverse Transcriptase Reagents bottle, positive control agent bottle are in low temperature environment (- 20
DEG C) storage;Cell pyrolysis liquid reagent bottle, Tris-HCl reagent bottles, mixed reaction solution reagent bottle, Taq enzyme reagent bottle, the moon are provided
Property contrast agents bottle wait low temperature environment (4 DEG C) storage, temperature constant state can be kept in transportation, it is ensured that experimental result
Accuracy.
Brief description of the drawings
Fig. 1 is the structural representation of B races streptococcus detection kit described in the utility model;
Fig. 2 is the top view of B races streptococcus detection kit box body described in the utility model;
Wherein, 1, box body;2nd, lid;3rd, primer kit bottle;4th, probe reagent bottle;5th, polymerase reagent bottle;6th, reverse transcription
Enzymatic reagent bottle;7th, positive control bottle;8th, Tris-HCl reagent bottles;9th, Taq enzyme reagent bottle;10th, mixed reaction solution reagent bottle;11、
Negative control bottle;12nd, cell pyrolysis liquid reagent bottle.
Embodiment
For the purpose of this utility model, technical scheme and advantage is better described, below in conjunction with accompanying drawing and specific implementation
Example is further described to the utility model.
In biological technical field, although kit is the important container for taking up reagent bottle.But, existing reagent
Box, such as RT-PCR diagnostic kits are usually to be made up of papery, and outside, which is coated, places buffering inside one layer of waterproof membrane, kit
Material, and kit has volume greatly, light-shading effect is poor, takes the cumbersome shortcoming of reagent.
For above-mentioned deficiency, as shown in figure 1, the utility model provides a kind of B races streptococcus detection kit, it includes
Box body 1 and lid 2, are placed with buffer body in the box body 1, and the buffer body is provided with multiple with detection reagent bottle diameter phase
Detection reagent bottle is placed with the shrinkage pool of matching, the shrinkage pool, the detection reagent bottle includes primer kit bottle 3, probe reagent
Bottle 4, t7 rna polymerase reagent bottle 5, Reverse Transcriptase Reagents bottle 6, positive control bottle 7, Tris-HCl reagent bottles 8, Taq enzyme reagent
Bottle 9, mixed reaction solution reagent bottle 10, negative control bottle 11 and cell pyrolysis liquid reagent bottle 12.
Preferably, the buffer body is foam-rubber cushion.
Preferably, whole kit is rectangular, more facilitates so as to carry and transport.
Preferably, the box body 1 is the box body being made up of the brown color polypropylene material of lucifuge.Because biological reagent is general
Need to be kept in dark place, but if directly from the reagent bottle storing reagent of brown color, in process of production or used
Preceding inconvenient people pass through reagent bottle observe interior reagent state, it is impossible to by naked eyes intuitively judge reagent whether muddiness or
For example there are quality problems in other problemses.And box body of the present invention is used after lucifuge material, reagent bottle can select clear vial
Body, so can be easy to observe the state of reagent again while reagent is kept in dark place.Wherein, brown color polypropylene material is existing
There is material, be commercially available.
In one embodiment, the box body 1 is divided into two rest areas by dividing plate, and one of rest area is placed with carefully
Cellular lysate liquid reagent bottle 12, another, which places two row's parallel exhausts of differentiation, other detection reagents bottle, wherein a discharge is equipped with and drawn
Thing reagent bottle 3, probe reagent bottle 4, polymerase reagent bottle 5, Reverse Transcriptase Reagents bottle 6, positive control bottle 7, another discharge is equipped with
Tris-HCl reagent bottles 8, Taq enzyme reagent bottle 9, mixed reaction solution reagent bottle 10, negative control bottle 11.Wherein, cell pyrolysis liquid
Reagent bottle is provided with 2.
Preferably, polyurethane thermal insulation board is provided with the box body 1, the polyurethane thermal insulation board is located at the bottom of the box body 1
In portion, four the week side of boss walls of box body 1 and the lid 2 inner side.
Preferably, it is described placement primer kit bottle 3, probe reagent bottle 4, polymerase reagent bottle 5, Reverse Transcriptase Reagents bottle 6,
1 inner side of the shrinkage pool of positive control bottle 7 is provided with polyurethane thermal insulation layer.
Preferably, it is additionally provided with ice bag in the box body.
Polyurethane thermal insulation board can play a part of thermal insulation protection, by using cooperatively for polyurethane adiabatic dividing plate and ice bag,
Ensure primer kit bottle, probe reagent bottle, polymerase reagent bottle, Reverse Transcriptase Reagents bottle, the storage temperature of positive control bottle be-
20℃;Ensure that Tris-HCl reagent bottles, Taq enzyme reagent bottle, mixed reaction solution reagent bottle, the storage temperature of negative control bottle are 4
DEG C, so as to ensure that each reagent can keep low temperature environment, it is ensured that the accuracy of use result, and the interior storage of traditional kit
Reagent, it is difficult to constant temperature is kept in transportation, influences whether experimental result.Preferably, the shrinkage pool top is in cylinder, its
Lower diameter is tapered into.
Preferably, sponge is filled with the lid 2, the top structure of the detection reagent bottle is embedded in sponge, the inspection
The bottom of test agent bottle is embedded in foam-rubber cushion.While conveniently taking reagent, the reagent in Reagent Tube is not easy to overflow.
Preferably, the detection reagent bottle is transparence, and the bottle wall of detection reagent bottle is provided with scale.So as to
Be visually known the remaining number of reagent bottle, exactly taken amount be used for test, do not result in waste reagent phenomenon.
Finally, it should be noted that above example is only to illustrate the technical solution of the utility model rather than to this reality
With the limitation of novel protected scope, although being explained in detail with reference to preferred embodiment to the utility model, this area it is common
It will be appreciated by the skilled person that can be modified to the technical solution of the utility model or equivalent substitution, without departing from this reality
With the spirit and scope of new technique scheme.
Claims (10)
1. a kind of B races streptococcus detection kit, it is characterised in that including box body and lid, be placed with the box body slow
Rush in body, the shrinkage pools that the buffer body matches provided with multiple and detection reagent bottle diameter, the shrinkage pool and be placed with detection examination
Agent bottle, the detection reagent bottle includes primer kit bottle, probe reagent bottle, t7 rna polymerase reagent bottle, Reverse Transcriptase Reagents
Bottle, positive control bottle, negative control bottle, Tris-HCl reagent bottles, Taq enzyme reagent bottle, mixed reaction solution reagent bottle and cell split
Solve liquid reagent bottle.
2. B races streptococcus detection kit as claimed in claim 1, it is characterised in that the box body is by the pale brown of lucifuge
The box body that color polypropylene material is made.
3. B races streptococcus detection kit as claimed in claim 1, it is characterised in that in the box body provided with polyurethane every
Hot plate, the polyurethane thermal insulation board on the bottom of the box body, four the week side of boss walls of box body and the lid inner side.
4. B races streptococcus detection kit as claimed in claim 3, it is characterised in that the placement primer kit bottle, probe
Reagent bottle, polymerase reagent bottle, Reverse Transcriptase Reagents bottle, the inner side of the shrinkage pool of positive control bottle are provided with polyurethane thermal insulation layer.
5. B races streptococcus detection kit as claimed in claim 1, it is characterised in that be additionally provided with ice bag in the box body.
6. B races streptococcus detection kit as claimed in claim 1, it is characterised in that the box body is divided into two by dividing plate
Individual rest area, one of rest area is placed with cell pyrolysis liquid reagent bottle, and another, which places two row's parallel exhausts of differentiation, it
His detection reagent bottle, wherein a discharge be equipped with primer kit bottle, probe reagent bottle, polymerase reagent bottle, Reverse Transcriptase Reagents bottle,
Positive control bottle, another discharge is equipped with Tris-HCl reagent bottles, Taq enzyme reagent bottle, mixed reaction solution reagent bottle, negative control
Bottle.
7. B races streptococcus detection kit as claimed in claim 1, it is characterised in that the shrinkage pool top is in cylinder, its
Lower diameter is tapered into.
8. B races streptococcus detection kit as described in any of claims 1, it is characterised in that filled in the lid
There is sponge, the top structure of the detection reagent bottle is embedded in sponge, and the bottom of the detection reagent bottle is embedded in buffer body.
9. such as B races streptococcus detection kit according to any one of claims 1 to 8, it is characterised in that the detection reagent
Bottle is transparence, and the bottle wall of detection reagent bottle is provided with scale.
10. such as B races streptococcus detection kit according to any one of claims 1 to 8, it is characterised in that the buffer body
For foam-rubber cushion.
Priority Applications (1)
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CN201621435072.XU CN206476989U (en) | 2016-12-26 | 2016-12-26 | A kind of B races streptococcus detection kit |
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CN201621435072.XU CN206476989U (en) | 2016-12-26 | 2016-12-26 | A kind of B races streptococcus detection kit |
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CN206476989U true CN206476989U (en) | 2017-09-08 |
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CN201621435072.XU Expired - Fee Related CN206476989U (en) | 2016-12-26 | 2016-12-26 | A kind of B races streptococcus detection kit |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN107525925A (en) * | 2017-10-12 | 2017-12-29 | 天津灵卫科技发展有限公司 | A kind of clenbuterol hydrochloride detection kit |
CN109502183A (en) * | 2018-12-11 | 2019-03-22 | 安徽同致生物工程股份有限公司 | A kind of multi-layer kit for medical treatment detection |
CN110646387A (en) * | 2019-09-17 | 2020-01-03 | 苏州康吉诊断试剂有限公司 | Kit for flow-type fluorescence detection method |
CN114410450A (en) * | 2022-01-10 | 2022-04-29 | 肖璐军 | Quick detect reagent box of microorganism |
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2016
- 2016-12-26 CN CN201621435072.XU patent/CN206476989U/en not_active Expired - Fee Related
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107525925A (en) * | 2017-10-12 | 2017-12-29 | 天津灵卫科技发展有限公司 | A kind of clenbuterol hydrochloride detection kit |
CN109502183A (en) * | 2018-12-11 | 2019-03-22 | 安徽同致生物工程股份有限公司 | A kind of multi-layer kit for medical treatment detection |
CN110646387A (en) * | 2019-09-17 | 2020-01-03 | 苏州康吉诊断试剂有限公司 | Kit for flow-type fluorescence detection method |
CN114410450A (en) * | 2022-01-10 | 2022-04-29 | 肖璐军 | Quick detect reagent box of microorganism |
CN114410450B (en) * | 2022-01-10 | 2024-02-09 | 浙江元勤生物医药科技有限公司 | Microorganism rapid detection kit |
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Granted publication date: 20170908 Termination date: 20211226 |
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