CN206476989U - A kind of B races streptococcus detection kit - Google Patents

A kind of B races streptococcus detection kit Download PDF

Info

Publication number
CN206476989U
CN206476989U CN201621435072.XU CN201621435072U CN206476989U CN 206476989 U CN206476989 U CN 206476989U CN 201621435072 U CN201621435072 U CN 201621435072U CN 206476989 U CN206476989 U CN 206476989U
Authority
CN
China
Prior art keywords
bottle
reagent bottle
box body
detection
reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201621435072.XU
Other languages
Chinese (zh)
Inventor
何凤屏
刘布
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN201621435072.XU priority Critical patent/CN206476989U/en
Application granted granted Critical
Publication of CN206476989U publication Critical patent/CN206476989U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The utility model discloses a kind of B races streptococcus detection kit, including box body and lid, buffer body is placed with box body, buffer body is provided with multiple shrinkage pools matched with detection reagent bottle diameter, detection reagent bottle is placed with shrinkage pool, detection reagent bottle includes primer kit bottle, probe reagent bottle, t7 rna polymerase reagent bottle, Reverse Transcriptase Reagents bottle, positive control bottle, negative control bottle, Tris HCl reagent bottles, Taq enzyme reagent bottle, mixed reaction solution reagent bottle and cell pyrolysis liquid reagent bottle, box body of the brown color polypropylene material of lucifuge by being made up, polyurethane thermal insulation board is provided with box body, polyurethane thermal insulation board is located at the bottom of box body, on four the week side of boss walls of box body and lid inner side.B races streptococcus detection kit energy described in the utility model is moistureproof, lucifuge, and kit storage occupies little space, convenient transportation;Keep low temperature environment and energy maximum possible is close to permanent low-temperature condition, be conducive to meeting movement requirement, it is ensured that the kit reaches optimum detection effect.

Description

A kind of B races streptococcus detection kit
Technical field
The utility model is related to a kind of detection kit, especially a kind of B races streptococcus RNA detection kits.
Background technology
B races streptococcus (Group B Streptococcus, abbreviation GBS) is a kind of conditioned pathogen, in perinatal infection In ranked first the pathogenic bacteria of position, perinatal infants dead rate can be caused.It is also the important pathogenic bacteria of pregnant and lying-in women's RTI simultaneously, can be with Cause the complication such as urinary system infection contamination, pregnant and lying-in women's septicemia.When bacterium develops into urogenital tract, and often and monilial infection Coexist.Pregnant woman's vagina bacterial bearing rate be 5~30%, pregnant woman's bacterial bearing rate with ethnic group, area, the age and it is different, in some special populations, As bacterial bearing rate in obesity, IGT, multiple pregnancies and the active women of sexual life is higher.Pregnant Women of Perinatal Period B races hammer The positive substantially increase intrauterine infection of bacterium infection has undesirable effect to pregnancy outcome:Premature rupture of fetal membranes (incidence of disease is 35%), amnion Chamber infection (incidence of disease is 21%), late abortion, premature labor, puerperal infection etc..B races streptococcal infection simultaneously can also cause neonate The generation of invasive infection.B races streptococcus can cause sepsis of the newborn, pneumonia, meningitis, or even dead.Deposit after infection Neonate living, it is also possible to have serious nervous system sequelae, including hydrocephalus, dysnoesia, microcephalus, deafness Deng.Meanwhile, B races streptococcus can also cause infection of pregnant women, cause premature labor, development of fetus bad (infant of low-birth weight), premature rupture of fetal membranes and Late abortion.Therefore, detection streptococcic to pregnant woman B races is extremely important and necessary.
At present, the streptococcic detection of B races mainly has following three kinds of methods:(1) traditional Bacteria Culture detection method, this method Testing result it is more accurate, but detection time is longer, typically needs that result could be come out within 24-76 hours, less efficient, it is impossible to meet In clinic;(2) although PCR detection methods, detection B races streptococcus DNA, detection method has higher sensitivity, still, detection When be easily caused intersection amplified reaction or pollution, so as to false positive results occur, so, the application of this method is also by very Big limitation;(3) collaurum detection method, this method is simple to operate there is provided qualitative detection result, but sensitivity is low, particularly If infective dose is few, it is possible that missing inspection, the situation of flase drop.
In biological technical field, kit is the important container for taking up reagent bottle.But, existing kit, example Such as RT-PCR diagnostic kits are usually and are made up of papery, and outside, which is coated, places padded coaming inside one layer of waterproof membrane, kit, And kit has volume greatly, light-shading effect is poor, takes the cumbersome shortcoming of reagent.
Utility model content
Based on the deficiencies in the prior art, the purpose of this utility model is to provide a kind of protection against the tide, lucifuge, keeps low temperature ring Border, maximum possible are close to temperature constant state, the B races streptococcus detection kit for being conducive to meeting movement requirement.
To achieve the above object, the technical scheme that the utility model is taken is:A kind of B races streptococcus detection kit, bag Box body and lid are included, buffer body is placed with the box body, the buffer body is provided with multiple with detection reagent bottle diameter phase Detection reagent bottle is placed with the shrinkage pool of matching, the shrinkage pool, the detection reagent bottle includes primer kit bottle, probe reagent Bottle, t7 rna polymerase reagent bottle, Reverse Transcriptase Reagents bottle, positive control bottle, negative control bottle, Tris-HCl reagent bottles, Taq Enzymatic reagent bottle, mixed reaction solution reagent bottle and cell pyrolysis liquid reagent bottle.
As the improvement of above-mentioned technical proposal, the box body is the box body being made up of the brown color polypropylene material of lucifuge. It is kept in dark place because biological reagent is generally required, but if directly from the reagent bottle storing reagent of brown color, in life The state of interior reagent is observed during production or using preceding inconvenient people by reagent bottle, it is impossible to intuitively sentence by naked eyes Whether with disconnected reagent if for example there are quality problems in muddy or other problemses.And box body of the present invention is used after lucifuge material, reagent Bottle can select transparent vial body, so can be easy to observe the state of reagent again while reagent is kept in dark place.
As the improvement of above-mentioned technical proposal, polyurethane thermal insulation board is provided with the box body, the polyurethane thermal insulation board is set In on the bottom of the box body, four the week side of boss walls of box body and the lid inner side.
It is the placement primer kit bottle, probe reagent bottle, polymerase reagent bottle, inverse as the improvement of above-mentioned technical proposal Transcriptase reagent bottle, the inner side of the shrinkage pool of positive control bottle are provided with polyurethane thermal insulation layer.
As the improvement of above-mentioned technical proposal, ice bag is additionally provided with the box body.
As the improvement of above-mentioned technical proposal, the box body is divided into two rest areas, one of rest area by dividing plate Cell pyrolysis liquid reagent bottle is placed with, another, which places two row's parallel exhausts of differentiation, other detection reagents bottle, wherein a discharge It is equipped with primer kit bottle, probe reagent bottle, polymerase reagent bottle, Reverse Transcriptase Reagents bottle, positive control bottle;Another discharge is equipped with Tris-HCl reagent bottles, Taq enzyme reagent bottle, mixed reaction solution reagent bottle, negative control bottle.
Polyurethane thermal insulation board can play a part of thermal insulation protection, by using cooperatively for polyurethane adiabatic dividing plate and ice bag, Ensure primer kit bottle, probe reagent bottle, polymerase reagent bottle, Reverse Transcriptase Reagents bottle, the storage temperature of positive control bottle be- 20℃;Ensure that Tris-HCl reagent bottles, Taq enzyme reagent bottle, mixed reaction solution reagent bottle, the storage temperature of negative control bottle are 4 DEG C, so as to ensure that each reagent can keep low temperature environment.
As the improvement of above-mentioned technical proposal, the shrinkage pool top is tapered into cylinder, its underpart diameter.It is so recessed Hole is combined closely with reagent bottle, and reagent bottle is difficult to skid off in transit.
As the improvement of above-mentioned technical proposal, sponge is filled with the lid, the top structure of the detection reagent bottle is embedding In sponge, the bottom of the detection reagent bottle is embedded in buffer body.
As the improvement of above-mentioned technical proposal, the buffer body is foam-rubber cushion.
As the improvement of above-mentioned technical proposal, detection reagent bottle is transparence, and the bottle of detection reagent bottle Wall is provided with scale.So as to be visually known the remaining number of reagent bottle, exactly taken amount be used for test.
The beneficial effects of the utility model are:The utility model provides a kind of B races streptococcus detection kit, the examination Agent box has advantages below:
(1) the moistureproof lucifuge of kit energy, and kit small volume, storage occupies little space, convenient transportation;
(2) low temperature environment is kept, and maximum possible is close to temperature constant state, is conducive to meeting the requirements in transit, it is ensured that should Kit reaches optimum detection effect;
(3) B races streptococcus detection kit of the present utility model is by setting thermal insulation layer and ice bag, it is possible to provide primer kit Bottle, probe reagent bottle, t7 rna polymerase reagent bottle, Reverse Transcriptase Reagents bottle, positive control agent bottle are in low temperature environment (- 20 DEG C) storage;Cell pyrolysis liquid reagent bottle, Tris-HCl reagent bottles, mixed reaction solution reagent bottle, Taq enzyme reagent bottle, the moon are provided Property contrast agents bottle wait low temperature environment (4 DEG C) storage, temperature constant state can be kept in transportation, it is ensured that experimental result Accuracy.
Brief description of the drawings
Fig. 1 is the structural representation of B races streptococcus detection kit described in the utility model;
Fig. 2 is the top view of B races streptococcus detection kit box body described in the utility model;
Wherein, 1, box body;2nd, lid;3rd, primer kit bottle;4th, probe reagent bottle;5th, polymerase reagent bottle;6th, reverse transcription Enzymatic reagent bottle;7th, positive control bottle;8th, Tris-HCl reagent bottles;9th, Taq enzyme reagent bottle;10th, mixed reaction solution reagent bottle;11、 Negative control bottle;12nd, cell pyrolysis liquid reagent bottle.
Embodiment
For the purpose of this utility model, technical scheme and advantage is better described, below in conjunction with accompanying drawing and specific implementation Example is further described to the utility model.
In biological technical field, although kit is the important container for taking up reagent bottle.But, existing reagent Box, such as RT-PCR diagnostic kits are usually to be made up of papery, and outside, which is coated, places buffering inside one layer of waterproof membrane, kit Material, and kit has volume greatly, light-shading effect is poor, takes the cumbersome shortcoming of reagent.
For above-mentioned deficiency, as shown in figure 1, the utility model provides a kind of B races streptococcus detection kit, it includes Box body 1 and lid 2, are placed with buffer body in the box body 1, and the buffer body is provided with multiple with detection reagent bottle diameter phase Detection reagent bottle is placed with the shrinkage pool of matching, the shrinkage pool, the detection reagent bottle includes primer kit bottle 3, probe reagent Bottle 4, t7 rna polymerase reagent bottle 5, Reverse Transcriptase Reagents bottle 6, positive control bottle 7, Tris-HCl reagent bottles 8, Taq enzyme reagent Bottle 9, mixed reaction solution reagent bottle 10, negative control bottle 11 and cell pyrolysis liquid reagent bottle 12.
Preferably, the buffer body is foam-rubber cushion.
Preferably, whole kit is rectangular, more facilitates so as to carry and transport.
Preferably, the box body 1 is the box body being made up of the brown color polypropylene material of lucifuge.Because biological reagent is general Need to be kept in dark place, but if directly from the reagent bottle storing reagent of brown color, in process of production or used Preceding inconvenient people pass through reagent bottle observe interior reagent state, it is impossible to by naked eyes intuitively judge reagent whether muddiness or For example there are quality problems in other problemses.And box body of the present invention is used after lucifuge material, reagent bottle can select clear vial Body, so can be easy to observe the state of reagent again while reagent is kept in dark place.Wherein, brown color polypropylene material is existing There is material, be commercially available.
In one embodiment, the box body 1 is divided into two rest areas by dividing plate, and one of rest area is placed with carefully Cellular lysate liquid reagent bottle 12, another, which places two row's parallel exhausts of differentiation, other detection reagents bottle, wherein a discharge is equipped with and drawn Thing reagent bottle 3, probe reagent bottle 4, polymerase reagent bottle 5, Reverse Transcriptase Reagents bottle 6, positive control bottle 7, another discharge is equipped with Tris-HCl reagent bottles 8, Taq enzyme reagent bottle 9, mixed reaction solution reagent bottle 10, negative control bottle 11.Wherein, cell pyrolysis liquid Reagent bottle is provided with 2.
Preferably, polyurethane thermal insulation board is provided with the box body 1, the polyurethane thermal insulation board is located at the bottom of the box body 1 In portion, four the week side of boss walls of box body 1 and the lid 2 inner side.
Preferably, it is described placement primer kit bottle 3, probe reagent bottle 4, polymerase reagent bottle 5, Reverse Transcriptase Reagents bottle 6, 1 inner side of the shrinkage pool of positive control bottle 7 is provided with polyurethane thermal insulation layer.
Preferably, it is additionally provided with ice bag in the box body.
Polyurethane thermal insulation board can play a part of thermal insulation protection, by using cooperatively for polyurethane adiabatic dividing plate and ice bag, Ensure primer kit bottle, probe reagent bottle, polymerase reagent bottle, Reverse Transcriptase Reagents bottle, the storage temperature of positive control bottle be- 20℃;Ensure that Tris-HCl reagent bottles, Taq enzyme reagent bottle, mixed reaction solution reagent bottle, the storage temperature of negative control bottle are 4 DEG C, so as to ensure that each reagent can keep low temperature environment, it is ensured that the accuracy of use result, and the interior storage of traditional kit Reagent, it is difficult to constant temperature is kept in transportation, influences whether experimental result.Preferably, the shrinkage pool top is in cylinder, its Lower diameter is tapered into.
Preferably, sponge is filled with the lid 2, the top structure of the detection reagent bottle is embedded in sponge, the inspection The bottom of test agent bottle is embedded in foam-rubber cushion.While conveniently taking reagent, the reagent in Reagent Tube is not easy to overflow.
Preferably, the detection reagent bottle is transparence, and the bottle wall of detection reagent bottle is provided with scale.So as to Be visually known the remaining number of reagent bottle, exactly taken amount be used for test, do not result in waste reagent phenomenon.
Finally, it should be noted that above example is only to illustrate the technical solution of the utility model rather than to this reality With the limitation of novel protected scope, although being explained in detail with reference to preferred embodiment to the utility model, this area it is common It will be appreciated by the skilled person that can be modified to the technical solution of the utility model or equivalent substitution, without departing from this reality With the spirit and scope of new technique scheme.

Claims (10)

1. a kind of B races streptococcus detection kit, it is characterised in that including box body and lid, be placed with the box body slow Rush in body, the shrinkage pools that the buffer body matches provided with multiple and detection reagent bottle diameter, the shrinkage pool and be placed with detection examination Agent bottle, the detection reagent bottle includes primer kit bottle, probe reagent bottle, t7 rna polymerase reagent bottle, Reverse Transcriptase Reagents Bottle, positive control bottle, negative control bottle, Tris-HCl reagent bottles, Taq enzyme reagent bottle, mixed reaction solution reagent bottle and cell split Solve liquid reagent bottle.
2. B races streptococcus detection kit as claimed in claim 1, it is characterised in that the box body is by the pale brown of lucifuge The box body that color polypropylene material is made.
3. B races streptococcus detection kit as claimed in claim 1, it is characterised in that in the box body provided with polyurethane every Hot plate, the polyurethane thermal insulation board on the bottom of the box body, four the week side of boss walls of box body and the lid inner side.
4. B races streptococcus detection kit as claimed in claim 3, it is characterised in that the placement primer kit bottle, probe Reagent bottle, polymerase reagent bottle, Reverse Transcriptase Reagents bottle, the inner side of the shrinkage pool of positive control bottle are provided with polyurethane thermal insulation layer.
5. B races streptococcus detection kit as claimed in claim 1, it is characterised in that be additionally provided with ice bag in the box body.
6. B races streptococcus detection kit as claimed in claim 1, it is characterised in that the box body is divided into two by dividing plate Individual rest area, one of rest area is placed with cell pyrolysis liquid reagent bottle, and another, which places two row's parallel exhausts of differentiation, it His detection reagent bottle, wherein a discharge be equipped with primer kit bottle, probe reagent bottle, polymerase reagent bottle, Reverse Transcriptase Reagents bottle, Positive control bottle, another discharge is equipped with Tris-HCl reagent bottles, Taq enzyme reagent bottle, mixed reaction solution reagent bottle, negative control Bottle.
7. B races streptococcus detection kit as claimed in claim 1, it is characterised in that the shrinkage pool top is in cylinder, its Lower diameter is tapered into.
8. B races streptococcus detection kit as described in any of claims 1, it is characterised in that filled in the lid There is sponge, the top structure of the detection reagent bottle is embedded in sponge, and the bottom of the detection reagent bottle is embedded in buffer body.
9. such as B races streptococcus detection kit according to any one of claims 1 to 8, it is characterised in that the detection reagent Bottle is transparence, and the bottle wall of detection reagent bottle is provided with scale.
10. such as B races streptococcus detection kit according to any one of claims 1 to 8, it is characterised in that the buffer body For foam-rubber cushion.
CN201621435072.XU 2016-12-26 2016-12-26 A kind of B races streptococcus detection kit Expired - Fee Related CN206476989U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201621435072.XU CN206476989U (en) 2016-12-26 2016-12-26 A kind of B races streptococcus detection kit

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201621435072.XU CN206476989U (en) 2016-12-26 2016-12-26 A kind of B races streptococcus detection kit

Publications (1)

Publication Number Publication Date
CN206476989U true CN206476989U (en) 2017-09-08

Family

ID=59749577

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201621435072.XU Expired - Fee Related CN206476989U (en) 2016-12-26 2016-12-26 A kind of B races streptococcus detection kit

Country Status (1)

Country Link
CN (1) CN206476989U (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107525925A (en) * 2017-10-12 2017-12-29 天津灵卫科技发展有限公司 A kind of clenbuterol hydrochloride detection kit
CN109502183A (en) * 2018-12-11 2019-03-22 安徽同致生物工程股份有限公司 A kind of multi-layer kit for medical treatment detection
CN110646387A (en) * 2019-09-17 2020-01-03 苏州康吉诊断试剂有限公司 Kit for flow-type fluorescence detection method
CN114410450A (en) * 2022-01-10 2022-04-29 肖璐军 Quick detect reagent box of microorganism

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107525925A (en) * 2017-10-12 2017-12-29 天津灵卫科技发展有限公司 A kind of clenbuterol hydrochloride detection kit
CN109502183A (en) * 2018-12-11 2019-03-22 安徽同致生物工程股份有限公司 A kind of multi-layer kit for medical treatment detection
CN110646387A (en) * 2019-09-17 2020-01-03 苏州康吉诊断试剂有限公司 Kit for flow-type fluorescence detection method
CN114410450A (en) * 2022-01-10 2022-04-29 肖璐军 Quick detect reagent box of microorganism
CN114410450B (en) * 2022-01-10 2024-02-09 浙江元勤生物医药科技有限公司 Microorganism rapid detection kit

Similar Documents

Publication Publication Date Title
CN206476989U (en) A kind of B races streptococcus detection kit
Satzke et al. Standard method for detecting upper respiratory carriage of Streptococcus pneumoniae: updated recommendations from the World Health Organization Pneumococcal Carriage Working Group
El Aila et al. Comparison of different sampling techniques and of different culture methods for detection of group B streptococcus carriage in pregnant women
Wang et al. Diagnosis and microecological characteristics of aerobic vaginitis in outpatients based on preformed enzymes
Kubota Relationship between maternal group B streptococcal colonization and pregnancy outcome
CN103409509B (en) Group B streptococcus fluorescence PCR detection kit
CN103773672B (en) Method and device for detecting motility and chemotaxis of human sperms
CN206087669U (en) Pseudomonas fluorescens detect reagent box
Vlaspolder et al. Value of a DNA probe assay (Gen-Probe) compared with that of culture for diagnosis of gonococcal infection
CN107227253A (en) A kind of culture device for anaerobic bacteria and cultural method
CN111455075A (en) L AMP primer group, microfluidic chip and kit for detecting pathogenic microorganisms in genital tract
CN205368340U (en) Microbial cultivation household utensils
Solt et al. The great obstetrical syndromes and the human microbiome—a new frontier
Aleshkin et al. Vaginal microbiota in healthy women and patients with bacterial vaginosis and nonspecific vaginitis
CN102417931B (en) Polymerase chain reaction (PCR) fluorescence detection kit and detection method for candida albicans
de Velasco-Sada et al. Evaluation of two real time PCR assays for the detection of bacterial DNA in amniotic fluid
JPH01252299A (en) Identification of enterobacteria in drinking water, bath water, waste water, bood and beverage
CN101163787A (en) Viral diagnostic method and well for use in same
CN103898191B (en) Detection Methods of Salmonella and detection substratum
CN203630045U (en) Neisseria gonorrhoeae joint examination card
CN213139575U (en) Nucleic acid purification centrifuging tube storage device
Al-Rashedi Evaluation of Candida albicans diagnosis by using conventional PCR
CN107988434A (en) Infectious bovine rhinotrachetis virus detection kit and application based on constant temperature isolation type fluorescent PCR platform
CN203728825U (en) Portable microorganism sampling culturing refrigeration device
Moussa et al. Group B streptococcus colonization of pregnant women: comparative molecular and microbiological diagnosis

Legal Events

Date Code Title Description
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20170908

Termination date: 20211226

CF01 Termination of patent right due to non-payment of annual fee