CN110628606A - PCR appearance calibrating device - Google Patents
PCR appearance calibrating device Download PDFInfo
- Publication number
- CN110628606A CN110628606A CN201910895549.4A CN201910895549A CN110628606A CN 110628606 A CN110628606 A CN 110628606A CN 201910895549 A CN201910895549 A CN 201910895549A CN 110628606 A CN110628606 A CN 110628606A
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- Prior art keywords
- temperature
- circuit board
- calibration device
- real
- fixing plate
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
- G01K13/00—Thermometers specially adapted for specific purposes
Abstract
The PCR instrument calibration device provided by the invention comprises a calibration device body and a temperature detection assembly; the calibration device body is electrically connected with the temperature detection assembly; the temperature detection assembly comprises a circuit board, a fixing plate and a temperature measurement probe; one surface of the circuit board is connected with one surface of the fixing plate; the other side of the circuit board is electrically connected with the temperature measuring probe; one end of the temperature measuring probe is positioned between the circuit board and the fixing plate, the other end of the temperature measuring probe is positioned below the fixing plate, and the temperature measuring probe measures a real-time temperature value and sends the real-time temperature value to the calibrating device body; the calibration device body is right according to preset rule the real-time temperature value is revised, obtains the temperature value after the revision, revises real-time temperature value through preset rule in addition, has improved the degree of accuracy of measured temperature, and through wiring at the another side of circuit board, it is more reasonable, optimize the wiring, be convenient for close the lid and measure.
Description
Technical Field
The invention relates to the technical field of medical equipment measurement, in particular to a PCR instrument calibration device.
Background
A Polymerase Chain Reaction Analyzer (PCR Analyzer for short), which is an apparatus for DNA Polymerase to make gene copy under the condition of specified temperature field.
At present, the PCR instrument still has the following problems:
1. the sensor has too large appearance, cannot be measured by closing a cover, and cannot faithfully react the actual working condition of the PCR instrument;
2. the sensor lead is too thin and can not be completely contacted with the heating part at the bottom of the PCR, so that the measured temperature is lower than the actual temperature, or the sensor lead is directly contacted with the upper cover of the instrument, and the measured temperature is far higher than the actual temperature due to the reaction of the hot cover;
3. the measuring points are many, and the wiring is complicated and is not easy to operate.
Disclosure of Invention
Technical problem to be solved
In order to solve the above problems in the prior art, the present invention provides a calibration apparatus for a PCR instrument, which has high accuracy and is convenient for wiring.
(II) technical scheme
In order to achieve the purpose, the invention adopts the main technical scheme that:
a PCR instrument calibration device comprises a calibration device body and a temperature detection assembly;
the calibration device body is electrically connected with the temperature detection assembly;
the temperature detection assembly comprises a circuit board, a fixing plate and a temperature measurement probe;
one surface of the circuit board is connected with one surface of the fixing plate;
the other side of the circuit board is electrically connected with the temperature measuring probe;
one end of the temperature measuring probe is positioned between the circuit board and the fixing plate, and the other end of the temperature measuring probe is positioned below the fixing plate;
the temperature measuring probe measures a real-time temperature value and sends the real-time temperature value to the calibrating device body;
and the calibration device body corrects the real-time temperature value according to a preset rule to obtain a corrected temperature value.
(III) advantageous effects
The invention has the beneficial effects that: the device comprises a calibration device body and a temperature detection assembly; the calibration device body is electrically connected with the temperature detection assembly; the temperature detection assembly comprises a circuit board, a fixing plate and a temperature measurement probe; one surface of the circuit board is connected with one surface of the fixing plate; the other side of the circuit board is electrically connected with the temperature measuring probe; one end of the temperature measuring probe is positioned between the circuit board and the fixing plate, the other end of the temperature measuring probe is positioned below the fixing plate, and the temperature measuring probe measures a real-time temperature value and sends the real-time temperature value to the calibrating device body; the calibration device body corrects the real-time temperature value according to a preset rule to obtain a corrected temperature value, corrects the real-time temperature value through the preset rule, improves the accuracy of temperature measurement, and optimizes wiring through wiring on the other surface of the circuit board to facilitate cover closing measurement.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a calibration apparatus for a PCR instrument according to an embodiment of the present invention;
FIG. 2 is an exploded view of the temperature sensing assembly and the flexible circuit board of the calibration apparatus for PCR instrument according to the embodiment of the present invention;
FIG. 3 is a schematic structural diagram of a temperature measuring probe of the calibrating apparatus for a PCR instrument according to the embodiment of the present invention.
[ description of reference ]
1: a PCR instrument calibration device; 2: calibrating the device body; 3: a temperature detection assembly; 4: a circuit board; 5: a fixing plate; 6: a temperature measuring probe; 7: a flexible circuit board; 8: a through hole; 9: connecting a lead; 10: a connecting member; 11: a temperature sensor; 12: a red copper shell; 13: a heat conducting portion; 14: a heat-resistant portion; 15: plating a gold layer; 16: a counterbore.
Detailed Description
For the purpose of better explaining the present invention and to facilitate understanding, the present invention will be described in detail by way of specific embodiments with reference to the accompanying drawings.
Referring to fig. 1 to 3, a calibration device for a PCR instrument includes a calibration device body and a temperature detection assembly;
the calibration device body is electrically connected with the temperature detection assembly;
the temperature detection assembly comprises a circuit board, a fixing plate and a temperature measurement probe;
one surface of the circuit board is connected with one surface of the fixing plate;
the other side of the circuit board is electrically connected with the temperature measuring probe;
one end of the temperature measuring probe is positioned between the circuit board and the fixing plate, and the other end of the temperature measuring probe is positioned below the fixing plate;
the temperature measuring probe measures a real-time temperature value and sends the real-time temperature value to the calibrating device body;
and the calibration device body corrects the real-time temperature value according to a preset rule to obtain a corrected temperature value.
From the above description, the beneficial effects of the present invention are: the device comprises a calibration device body and a temperature detection assembly; the calibration device body is electrically connected with the temperature detection assembly; the temperature detection assembly comprises a circuit board, a fixing plate and a temperature measurement probe; one surface of the circuit board is connected with one surface of the fixing plate; the other side of the circuit board is electrically connected with the temperature measuring probe; one end of the temperature measuring probe is positioned between the circuit board and the fixing plate, the other end of the temperature measuring probe is positioned below the fixing plate, and the temperature measuring probe measures a real-time temperature value and sends the real-time temperature value to the calibrating device body; the calibration device body corrects the real-time temperature value according to a preset rule to obtain a corrected temperature value, corrects the real-time temperature value through the preset rule, improves the accuracy of temperature measurement, and optimizes wiring through wiring on the other surface of the circuit board to facilitate cover closing measurement.
Further, the calibration device body corrects the real-time temperature value according to a preset rule, and the corrected temperature value is specifically:
the calibration device body receives the real-time temperature value, determines a corresponding correction value according to the real-time temperature value, and corrects the real-time temperature value according to the correction value to obtain a corrected temperature value.
According to the above description, the real-time temperature value is received, the corresponding correction value is determined according to the real-time temperature value, and the real-time temperature value is corrected according to the correction value to obtain the corrected temperature value, so that the measurement accuracy is improved.
Further, the temperature detection assembly further comprises a flexible circuit board;
the calibration device body is electrically connected with the temperature detection assembly through the flexible circuit board.
According to the above description, through setting up the flexible circuit board, the calibrating device body passes through the flexible circuit board with the temperature detection subassembly electricity is connected, is convenient for close the lid and measures to avoid the unreasonable unable closed condition of upper cover that leads to of artifical wiring, thereby improved the degree of accuracy of measuring the temperature.
Furthermore, the temperature measuring probe comprises a connecting wire, a connecting piece and a temperature sensor;
the circuit board is provided with a through hole;
one end of the connecting wire penetrates through the through hole to be electrically connected with the other surface of the circuit board, and the other end of the connecting wire penetrates through the connecting piece to be electrically connected with the temperature sensor.
From the above description, through setting up the through-hole, carry out the wiring at the another side of circuit board, not only can not influence the stability of being connected between circuit board and the fixed plate, optimize the wiring moreover, be convenient for close the lid and measure.
Furthermore, a counter bore is arranged at the position, corresponding to the through hole, on the fixing plate;
one end of the connecting piece is clamped between the circuit board and the fixing plate through the counter bore, and the other end of the connecting piece penetrates through the counter bore.
According to the above description, the fixing plate is provided with the counter bore, one end of the connecting piece is clamped between the circuit board and the fixing plate through the counter bore, and the other end of the connecting piece penetrates through the counter bore, so that the structure is simple and the stability is high.
Furthermore, the temperature measuring probe also comprises a red copper shell;
one end of the red copper shell is positioned in the connecting piece, and the other end of the red copper shell is positioned outside the connecting piece;
the temperature sensor is arranged in the red copper shell and is positioned at the other end of the red copper shell.
Furthermore, the red copper shell comprises a heat conduction part and a heat-resisting part, and one end close to the temperature sensor is the heat conduction part;
the red copper shell is internally filled with a heat conduction part formed by heat conduction silica gel;
the red copper shell is filled with a heat-resistant part formed by high-temperature-resistant glue.
According to the above description, the temperature sensor is arranged in the red copper shell and located at the other end of the red copper shell, the red copper shell is filled with the heat conduction part formed by the heat conduction silica gel, the heat conduction coefficient is large, the performance is stable, the heat conduction efficiency is high, the temperature conduction speed is increased, the red copper shell is filled with the heat-resistant part formed by the high-temperature-resistant gel, and the service life is prolonged.
Furthermore, a gold plating layer is arranged outside the red copper shell.
As can be seen from the above description, the gold plating layer is arranged outside the red copper shell, so that the red copper shell is not easy to corrode, and the service life and the stability of temperature detection are improved.
Example one
A PCR instrument calibration device 1 comprises a calibration device body 2 and a temperature detection component 3;
the calibration device body 2 is electrically connected with the temperature detection assembly 3;
the temperature detection assembly 3 comprises a circuit board 4, a fixing plate 5 and a temperature measuring probe 6;
one surface of the circuit board 4 is connected with one surface of the fixing plate 5;
the other surface of the circuit board 4 is electrically connected with the temperature measuring probe 6;
one end of the temperature measuring probe 6 is positioned between the circuit board 4 and the fixing plate 5, and the other end is positioned below the fixing plate 5;
the temperature measuring probe 6 measures a real-time temperature value and sends the real-time temperature value to the calibrating device body 2;
and the calibration device body 2 corrects the real-time temperature value according to a preset rule to obtain a corrected temperature value.
The calibration device body 2 corrects the real-time temperature value according to a preset rule, and the corrected temperature value is specifically as follows:
the calibration device body 2 receives the real-time temperature value, determines a corresponding correction value according to the real-time temperature value, and corrects the real-time temperature value according to the correction value to obtain a corrected temperature value.
Specifically, the correction values corresponding to 0 ℃, 30 ℃, 60 ℃, 90 ℃ and 120 ℃ were set as b1, b2, b3, b4 and b5, respectively, using standard oil tanks;
after receiving the real-time temperature value tc detected by any temperature probe 6, firstly judging which temperature interval tc belongs to, and then correcting the real-time temperature value according to the corrected value to obtain a corrected real value ts:
when the number of the temperature measuring probes 6 is multiple, the accuracy of temperature measurement is improved by linearly correcting the multiple real-time temperature values.
The fixing plate 5 is made of phenolic aldehyde laminated paper boards, so that the mechanical strength is good, and the fixing plate is not easy to deform after long-term use;
in this embodiment, the number of the temperature probes 6 is 15, and the number of the temperature probes 6 can be adjusted according to actual requirements;
the temperature detection assembly 3 further comprises a flexible circuit board 7;
the calibration device body 2 is electrically connected with the temperature detection assembly 3 through the flexible circuit board 7.
The temperature measuring probe 6 comprises a connecting wire 9, a connecting piece 10 and a temperature sensor 11;
the circuit board 4 is provided with a through hole 8;
one end of the connecting wire 9 passes through the through hole 8 and is electrically connected with the other surface of the circuit board 4, and the other end of the connecting wire passes through the connecting piece 10 and is electrically connected with the temperature sensor 11.
Specifically, the connecting piece is made of elastic plastic;
a counter bore 16 is arranged on the fixing plate 5 at a position corresponding to the through hole 8;
one end of the connector is clamped between the circuit board 4 and the fixing plate 5 through the counter bore 16, and the other end of the connector passes through the counter bore 16.
Specifically, the connecting piece is a double-layer cylindrical piece consisting of a first cylinder and a second cylinder, the diameter of the first cylinder is larger than that of the second cylinder, the shape of the first cylinder is matched with that of the counter bore 16, the first cylinder is clamped between the circuit board 4 and the fixing plate 5 and fixed through gluing, the stability is high, and the second cylinder penetrates through the counter bore 16;
the temperature measuring probe 6 also comprises a red copper shell 12;
one end of the red copper shell 12 is positioned in the connecting piece 10, and the other end is positioned outside the connecting piece 10;
the temperature sensor 11 is arranged in the red copper shell 12 and is positioned at the other end of the red copper shell 12.
The red copper shell 12 comprises a heat conduction part 13 and a heat-resistant part 14, and one end close to the temperature sensor 11 is the heat conduction part 13;
a heat conducting part 13 formed by heat conducting silica gel is filled in the red copper shell 12;
the red copper shell 12 is filled with a heat-resistant part 14 formed by high-temperature-resistant glue.
Specifically, the diameter of the heat conducting part of the red copper shell 12 is 2.3 mm, which facilitates increasing the speed of temperature conduction, thereby increasing the response speed of the temperature sensor.
The outer surface of the red copper shell 12 is provided with a gold plating layer 15.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all equivalent changes made by using the contents of the present specification and the drawings, or applied directly or indirectly to the related technical fields, are included in the scope of the present invention.
Claims (8)
1. A PCR instrument calibration device is characterized by comprising a calibration device body and a temperature detection assembly;
the calibration device body is electrically connected with the temperature detection assembly;
the temperature detection assembly comprises a circuit board, a fixing plate and a temperature measurement probe;
one surface of the circuit board is connected with one surface of the fixing plate;
the other side of the circuit board is electrically connected with the temperature measuring probe;
one end of the temperature measuring probe is positioned between the circuit board and the fixing plate, and the other end of the temperature measuring probe is positioned below the fixing plate;
the temperature measuring probe measures a real-time temperature value and sends the real-time temperature value to the calibrating device body;
and the calibration device body corrects the real-time temperature value according to a preset rule to obtain a corrected temperature value.
2. The calibration device for the PCR instrument according to claim 1, wherein the calibration device body corrects the real-time temperature value according to a preset rule, and the corrected temperature value is specifically:
the calibration device body receives the real-time temperature value, determines a corresponding correction value according to the real-time temperature value, and corrects the real-time temperature value according to the correction value to obtain a corrected temperature value.
3. The PCR instrument calibration device of claim 1, wherein the temperature detection assembly further comprises a flexible circuit board;
the calibration device body is electrically connected with the temperature detection assembly through the flexible circuit board.
4. The calibrating device for the PCR instrument according to claim 1, wherein the temperature measuring probe comprises a connecting wire, a connecting piece and a temperature sensor;
the circuit board is provided with a through hole;
one end of the connecting wire penetrates through the through hole to be electrically connected with the other surface of the circuit board, and the other end of the connecting wire penetrates through the connecting piece to be electrically connected with the temperature sensor.
5. The calibrating device for the PCR instrument according to claim 4, wherein a counter bore is formed in the fixing plate at a position corresponding to the through hole;
one end of the connecting piece is clamped between the circuit board and the fixing plate through the counter bore, and the other end of the connecting piece penetrates through the counter bore.
6. The calibrating device for the PCR instrument according to claim 4, wherein the temperature measuring probe further comprises a red copper shell;
one end of the red copper shell is positioned in the connecting piece, and the other end of the red copper shell is positioned outside the connecting piece;
the temperature sensor is arranged in the red copper shell and is positioned at the other end of the red copper shell.
7. The calibrating apparatus for PCR instrument according to claim 4, wherein the copper case includes a heat conducting portion and a heat-resistant portion, and the heat conducting portion is disposed at an end close to the temperature sensor;
the red copper shell is internally filled with a heat conduction part formed by heat conduction silica gel;
the red copper shell is filled with a heat-resistant part formed by high-temperature-resistant glue.
8. The PCR instrument calibration device according to claim 6, wherein the outer surface of the red copper shell is a gold plating layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910895549.4A CN110628606A (en) | 2019-09-21 | 2019-09-21 | PCR appearance calibrating device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910895549.4A CN110628606A (en) | 2019-09-21 | 2019-09-21 | PCR appearance calibrating device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110628606A true CN110628606A (en) | 2019-12-31 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910895549.4A Pending CN110628606A (en) | 2019-09-21 | 2019-09-21 | PCR appearance calibrating device |
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| Country | Link |
|---|---|
| CN (1) | CN110628606A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115127698A (en) * | 2022-08-25 | 2022-09-30 | 鲲鹏基因（北京）科技有限责任公司 | Waterproof temperature polling instrument for temperature calibration of polymerase chain reaction analyzer |
-
2019
- 2019-09-21 CN CN201910895549.4A patent/CN110628606A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115127698A (en) * | 2022-08-25 | 2022-09-30 | 鲲鹏基因（北京）科技有限责任公司 | Waterproof temperature polling instrument for temperature calibration of polymerase chain reaction analyzer |
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