CN110624271A - Chinese herbal medicine micro-molecule fermentation extraction process - Google Patents
Chinese herbal medicine micro-molecule fermentation extraction process Download PDFInfo
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Abstract
The invention discloses a Chinese herbal medicine micro-molecule fermentation extraction process, which relates to the technical field of Chinese herbal medicine extraction and comprises the following steps of, step three, conveying cleaned Chinese herbal medicine raw materials into a microwave drying sterilizer for drying and sterilization; conveying the dried and sterilized Chinese medicinal raw materials to a pulverizer for pulverizing; conveying the extracting solution obtained in the step five into a tubular high-speed centrifuge for primary solid-liquid separation to obtain primary extracting solution; seventhly, injecting the primary extracting solution into an ultrafiltration filter for filtering to prepare a secondary extracting solution; and step eight, inoculating the secondary extract into a culture medium according to the inoculation amount of 8-15%, and inoculating the secondary extract into a liquid fermentation culture medium taking intestinal probiotics as a matrix to prepare the mycoplasm of the traditional Chinese medicine extract.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicine extraction, in particular to a Chinese herbal medicine micro-molecule fermentation extraction process.
Background
Chinese herbal medicines are unique medicines used for preventing and treating diseases in the traditional Chinese medicine and are also important marks which are distinguished from other medicines in the traditional Chinese medicine. Chinese people have been exploring Chinese herbal medicines for thousands of years. Handed over, Shennong tasting hundreds of herbs, pioneering medicine, Shennong is honored as "Yaohuang". The Chinese medicine is mainly composed of plant medicine (root, stem, leaf and fruit), animal medicine (internal organs, skin, bone and organ, etc.) and mineral medicine. Since the plant herbs account for the majority of the Chinese herbs, the Chinese herbs are also called Chinese herbs. At present, the number of Chinese medicines used in various places is about 5000, and the prescription formed by combining various medicinal materials is even more than countless. Thousands of years of researches form an independent science, benedict, the traditional Chinese medicine application theory is unique, the traditional Chinese medicines can be generally prepared into granules, decoction pieces or extracted into Chinese herbal medicine molecules, the extraction period of the traditional Chinese medicines in the extraction process is long, meanwhile, the waste rate of the medicinal materials is high, the extraction purity is not high, the extraction efficiency is not high, and the extraction cost is increased.
Disclosure of Invention
The invention aims to solve the problems of long extraction period, high waste rate of medicinal materials, low extraction purity, low extraction efficiency and increased extraction cost in the prior art, and provides a micro-molecular fermentation extraction process for Chinese herbal medicines.
In order to achieve the purpose, the invention adopts the following technical scheme: a fermentation and extraction process of Chinese herbal medicine micro molecule comprises the following steps,
firstly, selecting traditional Chinese medicine raw materials to be processed;
conveying the selected traditional Chinese medicine raw materials to a drum-type medicine washing machine, and washing the traditional Chinese medicine raw materials through the drum-type medicine washing machine;
conveying the cleaned traditional Chinese medicine raw materials into a microwave drying and sterilizing machine for drying and sterilizing;
conveying the dried and sterilized Chinese medicinal raw materials to a pulverizer for pulverizing;
step five, adding 40-70% of solvent into the crushed raw materials, and then conveying the mixed solution into a microwave extractor for extraction;
conveying the extracting solution obtained in the step five into a tubular high-speed centrifuge for primary solid-liquid separation to obtain primary extracting solution;
seventhly, injecting the primary extracting solution into an ultrafiltration filter for filtering to prepare a secondary extracting solution;
step eight, inoculating the secondary liquid extract into a culture medium according to the inoculation amount of 8-15%, and inoculating the secondary liquid extract into a liquid fermentation culture medium taking intestinal probiotics as a matrix to prepare the mycoplasm of the traditional Chinese medicine extract;
and step nine, injecting the mycoplasm of the traditional Chinese medicine extracting solution into a concentration tank, and performing concentration, rectification and extraction.
Preferably, the specific process parameters of the second step are as follows: the cleaning time is 45-90 minutes, and the rotating speed of the drum-type medicine washing machine is 500-.
Preferably, the specific process parameters of the third step are as follows: the drying temperature is 70-110 ℃, and the drying time is 25-60 minutes.
Preferably, the specific process parameters of the fourth step are as follows: crushing by a TF-400 type firewood field type crusher at the rotating speed of 2000-.
Preferably, the specific process parameters of the fifth step are as follows: the solvent is 20-50% ethanol, the power of the microwave extraction instrument is 200-700W, the temperature is 120-200 ℃, and the time is 10-40 min.
Preferably, the specific process parameters in the sixth step are as follows: the rotation speed of the tubular centrifuge is 2000-4500r/min, the time is 27-50 minutes, and the temperature is 30-60 ℃.
Preferably, the specific process of the seventh step is as follows: injecting the first-stage extractive solution into ultrafiltration filter, allowing the first-stage extractive solution to pass through ultrafiltration membrane with aperture of 1-20nm, and filtering with ultrafiltration membrane to obtain second-stage extractive solution with 5-100nm Chinese medicinal particles.
Preferably, the specific process of the step eight is as follows: inoculating the traditional Chinese medicine extracting solution into a culture medium according to the inoculation amount of 8-15%, storing the traditional Chinese medicine extracting solution in the culture medium according to the size of 6 x 6mm, inoculating the traditional Chinese medicine extracting solution into a liquid fermentation culture medium taking intestinal probiotics as a matrix, and culturing for 20-27 days under the conditions that the temperature is 28-50 ℃ and the rotating speed is 180-280r/min to obtain the mycoplasm of the traditional Chinese medicine extracting solution.
Preferably, the intestinal probiotic bacteria are one of bifidobacterium lactis, bifidobacterium longum, bifidobacterium breve, lactobacillus rhamnosus LGG, lactobacillus acidophilus NCFM or lactobacillus reuteri.
The invention has the beneficial effects that:
according to the invention, the procedures of screening, cleaning, drying and sterilizing the herbal medicine raw materials are carried out, so that mould and large-particle impurities in the herbal medicine raw materials can be separated from the herbal medicine, the later-stage processing of the herbal medicine raw materials is improved, the secondary filtering procedure is adopted, the particles in the traditional Chinese medicine extracting solution can be separated from the extracting solution, the later-stage extraction purity of herbal medicine molecules is improved, meanwhile, the waste rate of the traditional Chinese medicine raw materials is reduced, and further, the production cost is reduced.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments.
Example 1, a process for extracting Chinese herbal medicine micro-molecule by fermentation comprises the following steps,
firstly, selecting traditional Chinese medicine raw materials to be processed;
conveying the selected traditional Chinese medicine raw materials to a drum-type medicine washing machine, and washing the traditional Chinese medicine raw materials through the drum-type medicine washing machine, wherein the washing time is 45 minutes, and the rotating speed of the drum-type medicine washing machine is 500 r/min;
conveying the cleaned traditional Chinese medicine raw materials into a microwave drying and sterilizing machine for drying and sterilizing, wherein the drying temperature is 70 ℃, and the drying time is 25 minutes;
conveying the dried and sterilized Chinese medicinal raw materials to a TF-400 type firewood type pulverizer for pulverizing at the rotating speed of 2000r/min for 30 minutes;
adding 20% ethanol into the crushed raw materials, and conveying the mixed solution to a microwave extractor for extraction, wherein the power of the microwave extractor is 200W, the temperature is 120 ℃, and the time is 10 min;
step six, conveying the extracting solution obtained in the step five into a tubular high-speed centrifuge for primary solid-liquid separation to obtain primary extracting solution, wherein the rotating speed is 2000r/min, the time is 27 minutes, and the temperature is 30 ℃;
seventhly, injecting the primary extracting solution into an ultrafiltration filter, and filtering the primary extracting solution through an ultrafiltration membrane with the aperture of 1nm and 5nm of traditional Chinese medicine particles by using the ultrafiltration membrane to obtain a secondary extracting solution;
step eight, inoculating the traditional Chinese medicine extracting solution into a culture medium according to the inoculation amount of 8%, storing the traditional Chinese medicine extracting solution in the culture medium according to the size of 6 multiplied by 6mm, inoculating the traditional Chinese medicine extracting solution into a liquid fermentation culture medium taking intestinal probiotics as a matrix, and culturing for 20 days at the temperature of 28 ℃ and the rotating speed of 180r/min to obtain the mycoplasm of the traditional Chinese medicine extracting solution;
and step nine, injecting the mycoplasm of the traditional Chinese medicine extracting solution into a concentration tank, and performing concentration, rectification and extraction.
Specifically, the intestinal probiotic is one of Bifidobacterium lactis, Bifidobacterium longum, Bifidobacterium breve, Lactobacillus rhamnosus LGG, Lactobacillus acidophilus NCFM or Lactobacillus reuteri.
The following data were obtained by orthogonal experiments:
| name of item | Bifidobacterium lactis | Bifidobacterium longum | Bifidobacterium breve | Lactobacillus rhamnosus LGG | Lactobacillus acidophilus NCFM | Lactobacillus reuteri |
| Ratio of material to liquid | 1:5 | 1:7 | 1:6 | 1:9 | 1:10 | 1:12 |
Example 2, a process for extracting Chinese herbal medicine micro-molecule by fermentation comprises the following steps,
firstly, selecting traditional Chinese medicine raw materials to be processed;
conveying the selected traditional Chinese medicine raw materials to a drum-type medicine washing machine, and washing the traditional Chinese medicine raw materials through the drum-type medicine washing machine, wherein the washing time is 50 minutes, and the rotating speed of the drum-type medicine washing machine is 700 r/min;
conveying the cleaned traditional Chinese medicine raw materials into a microwave drying and sterilizing machine for drying and sterilizing, wherein the drying temperature is 75 ℃, and the drying time is 30 minutes;
conveying the dried and sterilized Chinese medicinal material raw materials to a TF-400 type firewood type grinder for grinding at the rotating speed of 2800r/min for 33 minutes;
adding 23% of ethanol into the crushed raw materials, and conveying the mixed solution to a microwave extractor for extraction, wherein the power of the microwave extractor is 250W, the temperature is 125 ℃, and the time is 15 min;
step six, conveying the extracting solution obtained in the step five into a tubular high-speed centrifuge for primary solid-liquid separation to obtain primary extracting solution, wherein the rotating speed is 2500r/min, the time is 30 minutes, and the temperature is 35 ℃;
seventhly, injecting the primary extracting solution into an ultrafiltration filter, and filtering the primary extracting solution through an ultrafiltration membrane with the aperture of 5nm and traditional Chinese medicine particles with the aperture of 15nm by using the ultrafiltration membrane to obtain a secondary extracting solution;
step eight, inoculating the traditional Chinese medicine extracting solution into a culture medium according to the inoculation amount of 9%, storing the traditional Chinese medicine extracting solution in the culture medium according to the size of 6 x 6mm, inoculating the traditional Chinese medicine extracting solution into a liquid fermentation culture medium taking intestinal probiotics as a matrix, and culturing for 21 days at the temperature of 30 ℃ and the rotating speed of 190r/min to obtain the mycoplasm of the traditional Chinese medicine extracting solution;
and step nine, injecting the mycoplasm of the traditional Chinese medicine extracting solution into a concentration tank, and performing concentration, rectification and extraction.
Specifically, the intestinal probiotic is one of Bifidobacterium lactis, Bifidobacterium longum, Bifidobacterium breve, Lactobacillus rhamnosus LGG, Lactobacillus acidophilus NCFM or Lactobacillus reuteri.
The following data were obtained by orthogonal experiments:
| name of item | Bifidobacterium lactis | Bifidobacterium longum | Bifidobacterium breve | Lactobacillus rhamnosus LGG | Lactobacillus acidophilus NCFM | Lactobacillus reuteri |
| Ratio of material to liquid | 1:4 | 1:6 | 1:5 | 1:8 | 1:9 | 1:11 |
Example 3, a process for extracting Chinese herbal medicine micro-molecule by fermentation comprises the following steps,
firstly, selecting traditional Chinese medicine raw materials to be processed;
conveying the selected traditional Chinese medicine raw materials to a drum-type medicine washing machine, and washing the traditional Chinese medicine raw materials through the drum-type medicine washing machine, wherein the washing time is 55 minutes, and the rotating speed of the drum-type medicine washing machine is 1000 r/min;
conveying the cleaned traditional Chinese medicine raw materials into a microwave drying and sterilizing machine for drying and sterilizing, wherein the drying temperature is 80 ℃, and the drying time is 38 minutes;
conveying the dried and sterilized Chinese medicinal raw materials to a TF-400 type firewood type pulverizer for pulverizing at a rotating speed of 3000r/min for 38 minutes;
adding 30% ethanol into the crushed raw materials, and conveying the mixed solution to a microwave extractor for extraction, wherein the power of the microwave extractor is 300W, the temperature is 140 ℃, and the time is 20 min;
step six, conveying the extracting solution obtained in the step five into a tubular high-speed centrifuge for primary solid-liquid separation to obtain primary extracting solution, wherein the rotating speed is 2800r/min, the time is 35 minutes, and the temperature is 38 ℃;
seventhly, injecting the primary extracting solution into an ultrafiltration filter, and filtering the primary extracting solution through an ultrafiltration membrane with the aperture of 8nm and 20nm of traditional Chinese medicine particles by using the ultrafiltration membrane to obtain a secondary extracting solution;
step eight, inoculating the traditional Chinese medicine extracting solution into a culture medium according to the inoculation amount of 10%, storing the traditional Chinese medicine extracting solution in the culture medium according to the size of 6 x 6mm, inoculating the traditional Chinese medicine extracting solution into a liquid fermentation culture medium taking intestinal probiotics as a matrix, and culturing for 22 days under the conditions that the temperature is 33 ℃ and the rotating speed is 210r/min to obtain the mycoplasm of the traditional Chinese medicine extracting solution;
and step nine, injecting the mycoplasm of the traditional Chinese medicine extracting solution into a concentration tank, and performing concentration, rectification and extraction.
Specifically, the intestinal probiotic is one of Bifidobacterium lactis, Bifidobacterium longum, Bifidobacterium breve, Lactobacillus rhamnosus LGG, Lactobacillus acidophilus NCFM or Lactobacillus reuteri.
The following data were obtained by orthogonal experiments:
| name of item | Bifidobacterium lactis | Bifidobacterium longum | Bifidobacterium breve | Lactobacillus rhamnosus LGG | Lactobacillus acidophilus NCFM | Lactobacillus reuteri |
| Ratio of material to liquid | 1:3 | 1:5 | 1:4 | 1:7 | 1:8 | 1:10 |
Example 4, a process for extracting Chinese herbal medicine micro-molecule by fermentation comprises the following steps,
firstly, selecting traditional Chinese medicine raw materials to be processed;
conveying the selected traditional Chinese medicine raw materials to a drum-type medicine washing machine, and washing the traditional Chinese medicine raw materials through the drum-type medicine washing machine, wherein the washing time is 60 minutes, and the rotating speed of the drum-type medicine washing machine is 1200 r/min;
conveying the cleaned traditional Chinese medicine raw materials into a microwave drying and sterilizing machine for drying and sterilizing, wherein the drying temperature is 85 ℃, and the drying time is 40 minutes;
conveying the dried and sterilized Chinese medicinal material raw materials to a TF-400 type firewood type grinder for grinding at the rotating speed of 3300r/min for 40 minutes;
adding 35% ethanol into the crushed raw materials, and conveying the mixed solution to a microwave extractor for extraction, wherein the power of the microwave extractor is 350W, the temperature is 150 ℃, and the time is 22 min;
step six, conveying the extracting solution obtained in the step five into a tubular high-speed centrifuge for primary solid-liquid separation to obtain primary extracting solution, wherein the rotating speed is 3000r/min, the time is 38 minutes, and the temperature is 40 ℃;
seventhly, injecting the primary extracting solution into an ultrafiltration filter, and filtering the primary extracting solution through an ultrafiltration membrane with the aperture of 12nm and the ultrafiltration membrane to obtain a secondary extracting solution, wherein the traditional Chinese medicine particles with the diameter of 30nm are filtered by the ultrafiltration membrane;
step eight, inoculating the traditional Chinese medicine extracting solution into a culture medium according to the inoculation amount of 11%, storing the traditional Chinese medicine extracting solution in the culture medium according to the size of 6 x 6mm, inoculating the traditional Chinese medicine extracting solution into a liquid fermentation culture medium taking intestinal probiotics as a matrix, and culturing for 23 days under the conditions that the temperature is 36 ℃ and the rotating speed is 220r/min to obtain the mycoplasm of the traditional Chinese medicine extracting solution;
and step nine, injecting the mycoplasm of the traditional Chinese medicine extracting solution into a concentration tank, and performing concentration, rectification and extraction.
The following data were obtained by orthogonal experiments:
| name of item | Bifidobacterium lactis | Bifidobacterium longum | Bifidobacterium breve | Lactobacillus rhamnosus LGG | Lactobacillus acidophilus NCFM | Lactobacillus reuteri |
| Ratio of material to liquid | 1:2 | 1:4 | 1:3 | 1:6 | 1:7 | 1:9 |
Example 5, a process for extracting Chinese herbal medicine micro-molecule by fermentation comprises the following steps,
firstly, selecting traditional Chinese medicine raw materials to be processed;
conveying the selected traditional Chinese medicine raw materials to a drum-type medicine washing machine, and washing the traditional Chinese medicine raw materials through the drum-type medicine washing machine, wherein the washing time is 70 minutes, and the rotating speed of the drum-type medicine washing machine is 1400 r/min;
conveying the cleaned traditional Chinese medicine raw materials into a microwave drying and sterilizing machine for drying and sterilizing, wherein the drying temperature is 90 ℃, and the drying time is 45 minutes;
conveying the dried and sterilized Chinese medicinal material raw materials to a TF-400 type firewood type pulverizer for pulverizing at a rotation speed of 3500r/min for 45 minutes;
step five, adding 40% ethanol into the crushed raw materials, and then conveying the mixed solution into a microwave extractor for extraction, wherein the power of the microwave extractor is 400W, the temperature is 170 ℃, and the time is 33 min;
step six, conveying the extracting solution obtained in the step five into a tubular high-speed centrifuge for primary solid-liquid separation to obtain primary extracting solution, wherein the rotating speed is 3500r/min, the time is 40 minutes, and the temperature is 45 ℃;
seventhly, injecting the primary extracting solution into an ultrafiltration filter, and filtering the primary extracting solution through an ultrafiltration membrane with the aperture of 15nm and the ultrafiltration membrane to obtain a secondary extracting solution, wherein the traditional Chinese medicine particles with the diameter of 50nm are filtered by the ultrafiltration membrane;
step eight, inoculating the traditional Chinese medicine extracting solution into a culture medium according to the inoculation amount of 12%, storing the traditional Chinese medicine extracting solution in the culture medium according to the size of 6 x 6mm, inoculating the traditional Chinese medicine extracting solution into a liquid fermentation culture medium taking intestinal probiotics as a matrix, and culturing for 25 days at the temperature of 40 ℃ and the rotating speed of 250r/min to obtain the mycoplasm of the traditional Chinese medicine extracting solution;
and step nine, injecting the mycoplasm of the traditional Chinese medicine extracting solution into a concentration tank, and performing concentration, rectification and extraction.
Specifically, the intestinal probiotic is one of Bifidobacterium lactis, Bifidobacterium longum, Bifidobacterium breve, Lactobacillus rhamnosus LGG, Lactobacillus acidophilus NCFM or Lactobacillus reuteri.
The following data were obtained by orthogonal experiments:
| name of item | Bifidobacterium lactis | Bifidobacterium longum | Bifidobacterium breve | Lactobacillus rhamnosus LGG | Lactobacillus acidophilus NCFM | Lactobacillus reuteri |
| Ratio of material to liquid | 1:2.5 | 1:4.5 | 1:3.5 | 1:6.5 | 1:7.5 | 1:9.5 |
Example 6, a process for extracting Chinese herbal medicine micro-molecule by fermentation comprises the following steps,
firstly, selecting traditional Chinese medicine raw materials to be processed;
conveying the selected traditional Chinese medicine raw materials to a drum-type medicine washing machine, and washing the traditional Chinese medicine raw materials through the drum-type medicine washing machine, wherein the washing time is 80 minutes, and the rotating speed of the drum-type medicine washing machine is 1800 r/min;
conveying the cleaned traditional Chinese medicine raw materials into a microwave drying and sterilizing machine for drying and sterilizing, wherein the drying temperature is 100 ℃, and the drying time is 50 minutes;
conveying the dried and sterilized Chinese medicinal raw materials to a TF-400 type firewood type pulverizer for pulverizing at the rotating speed of 4500r/min for 50 minutes;
step five, adding 45% ethanol into the crushed raw materials, and then conveying the mixed solution into a microwave extractor for extraction, wherein the power of the microwave extractor is 500W, the temperature is 180 ℃, and the time is 36 min;
step six, conveying the extracting solution obtained in the step five into a tubular high-speed centrifuge for primary solid-liquid separation to obtain primary extracting solution, wherein the rotating speed is 4000r/min, the time is 45 minutes, and the temperature is 50 ℃;
seventhly, injecting the primary extracting solution into an ultrafiltration filter, and filtering the primary extracting solution through an ultrafiltration membrane with the aperture of 18nm and traditional Chinese medicine particles with the aperture of 80nm by using the ultrafiltration membrane to obtain a secondary extracting solution;
step eight, inoculating the traditional Chinese medicine extracting solution into a culture medium according to the inoculation amount of 13%, storing the traditional Chinese medicine extracting solution in the culture medium according to the size of 6 x 6mm, inoculating the traditional Chinese medicine extracting solution into a liquid fermentation culture medium taking intestinal probiotics as a matrix, and culturing for 26 days at the temperature of 45 ℃ and the rotating speed of 260r/min to obtain the mycoplasm of the traditional Chinese medicine extracting solution;
and step nine, injecting the mycoplasm of the traditional Chinese medicine extracting solution into a concentration tank, and performing concentration, rectification and extraction.
Specifically, the intestinal probiotic is one of Bifidobacterium lactis, Bifidobacterium longum, Bifidobacterium breve, Lactobacillus rhamnosus LGG, Lactobacillus acidophilus NCFM or Lactobacillus reuteri.
The following data were obtained by orthogonal experiments:
| name of item | Bifidobacterium lactis | Bifidobacterium longum | Bifidobacterium breve | Lactobacillus rhamnosus LGG | Lactobacillus acidophilus NCFM | Lactobacillus reuteri |
| Ratio of material to liquid | 1:2.3 | 1:4.3 | 1:3.2 | 1:6.4 | 1:7.6 | 1:9.8 |
Example 7, a process for extracting Chinese herbal medicine micro-molecule by fermentation, comprising the following steps,
firstly, selecting traditional Chinese medicine raw materials to be processed;
conveying the selected traditional Chinese medicine raw materials to a drum-type medicine washing machine, and washing the traditional Chinese medicine raw materials through the drum-type medicine washing machine, wherein the washing time is 90 minutes, and the rotating speed of the drum-type medicine washing machine is 2000 r/min;
conveying the cleaned traditional Chinese medicine raw materials into a microwave drying and sterilizing machine for drying and sterilizing, wherein the drying temperature is 110 ℃, and the drying time is 60 minutes;
conveying the dried and sterilized Chinese medicinal raw materials to a TF-400 type firewood type pulverizer for pulverizing at the rotating speed of 5000r/min for 55 minutes;
adding 50% ethanol into the crushed raw materials, and conveying the mixed solution to a microwave extractor for extraction, wherein the power of the microwave extractor is 700W, the temperature is 200 ℃, and the time is 40 min;
step six, conveying the extracting solution obtained in the step five into a tubular high-speed centrifuge for primary solid-liquid separation to obtain primary extracting solution, wherein the rotating speed is 4500r/min, the time is 50 minutes, and the temperature is 60 ℃;
seventhly, injecting the primary extracting solution into an ultrafiltration filter, and filtering the primary extracting solution through an ultrafiltration membrane with the aperture of 20nm and the ultrafiltration membrane to obtain a secondary extracting solution, wherein the traditional Chinese medicine particles with the aperture of 100nm are filtered by the ultrafiltration membrane;
step eight, inoculating the traditional Chinese medicine extracting solution into a culture medium according to the inoculation amount of 15%, storing the traditional Chinese medicine extracting solution in the culture medium according to the size of 6 x 6mm, inoculating the traditional Chinese medicine extracting solution into a liquid fermentation culture medium taking intestinal probiotics as a matrix, and culturing for 27 days under the conditions that the temperature is 50 ℃ and the rotating speed is 280r/min to obtain the mycoplasm of the traditional Chinese medicine extracting solution;
and step nine, injecting the mycoplasm of the traditional Chinese medicine extracting solution into a concentration tank, and performing concentration, rectification and extraction.
Specifically, the intestinal probiotic is one of Bifidobacterium lactis, Bifidobacterium longum, Bifidobacterium breve, Lactobacillus rhamnosus LGG, Lactobacillus acidophilus NCFM or Lactobacillus reuteri.
The following data were obtained by orthogonal experiments:
| name of item | Bifidobacterium lactis | Bifidobacterium longum | Bifidobacterium breve | Lactobacillus rhamnosus LGG | Lactobacillus acidophilus NCFM | Lactobacillus reuteri |
| Ratio of material to liquid | 1:2.4 | 1:4.4 | 1:3.3 | 1:6.3 | 1:7.2 | 1:9.9 |
In conclusion, example 4 achieves the best extraction effect and the best extraction purity by comparing example 1, example 2, example 3, example 4, example 5, example 6 with example 7.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (9)
1. A fermentation and extraction process of Chinese herbal medicine micro-molecules is characterized by comprising the following steps,
firstly, selecting traditional Chinese medicine raw materials to be processed;
conveying the selected traditional Chinese medicine raw materials to a drum-type medicine washing machine, and washing the traditional Chinese medicine raw materials through the drum-type medicine washing machine;
conveying the cleaned traditional Chinese medicine raw materials into a microwave drying and sterilizing machine for drying and sterilizing;
conveying the dried and sterilized Chinese medicinal raw materials to a pulverizer for pulverizing;
step five, adding 40-70% of solvent into the crushed raw materials, and then conveying the mixed solution into a microwave extractor for extraction;
conveying the extracting solution obtained in the step five into a tubular high-speed centrifuge for primary solid-liquid separation to obtain primary extracting solution;
seventhly, injecting the primary extracting solution into an ultrafiltration filter for filtering to prepare a secondary extracting solution;
step eight, inoculating the secondary liquid extract into a culture medium according to the inoculation amount of 8-15%, and inoculating the secondary liquid extract into a liquid fermentation culture medium taking intestinal probiotics as a matrix to prepare the mycoplasm of the traditional Chinese medicine extract;
and step nine, injecting the mycoplasm of the traditional Chinese medicine extracting solution into a concentration tank, and performing concentration, rectification and extraction.
2. The fermentation and extraction process of Chinese herbal medicine micro molecules as claimed in claim 1, wherein the specific process parameters of the second step are as follows: the cleaning time is 45-90 minutes, and the rotating speed of the drum-type medicine washing machine is 500-.
3. The fermentation and extraction process of Chinese herbal medicine micro molecules as claimed in claim 1, wherein the specific process parameters of the third step are as follows: the drying temperature is 70-110 ℃, and the drying time is 25-60 minutes.
4. The fermentation and extraction process of Chinese herbal medicine micro molecules as claimed in claim 1, wherein the specific process parameters of the fourth step are as follows: crushing by a TF-400 type firewood field type crusher at the rotating speed of 2000-.
5. The fermentation and extraction process of Chinese herbal medicine micro molecules as claimed in claim 1, wherein the specific process parameters of the fifth step are as follows: the solvent is 20-50% ethanol, the power of the microwave extraction instrument is 200-700W, the temperature is 120-200 ℃, and the time is 10-40 min.
6. The fermentation and extraction process of Chinese herbal medicine micro molecules as claimed in claim 1, wherein the specific process parameters of the sixth step are as follows: the rotation speed of the tubular centrifuge is 2000-4500r/min, the time is 27-50 minutes, and the temperature is 30-60 ℃.
7. The fermentation and extraction process of Chinese herbal medicine micro molecules as claimed in claim 1, wherein the specific process of the seventh step is as follows: injecting the first-stage extractive solution into ultrafiltration filter, allowing the first-stage extractive solution to pass through ultrafiltration membrane with aperture of 1-20nm, and filtering with ultrafiltration membrane to obtain second-stage extractive solution with 5-100nm Chinese medicinal particles.
8. The fermentation and extraction process of Chinese herbal medicine micro molecules as claimed in claim 1, wherein the specific process of step eight is as follows: inoculating the traditional Chinese medicine extracting solution into a culture medium according to the inoculation amount of 8-15%, storing the traditional Chinese medicine extracting solution in the culture medium according to the size of 6 x 6mm, inoculating the traditional Chinese medicine extracting solution into a liquid fermentation culture medium taking intestinal probiotics as a matrix, and culturing for 20-27 days under the conditions that the temperature is 28-50 ℃ and the rotating speed is 180-280r/min to obtain the mycoplasm of the traditional Chinese medicine extracting solution.
9. The process of claim 1, wherein the intestinal probiotic is one of Bifidobacterium lactis, Bifidobacterium longum, Bifidobacterium breve, Lactobacillus rhamnosus LGG, Lactobacillus acidophilus NCFM or Lactobacillus reuteri.
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