CN110615719A - Preparation method and application of biofertilizer taking kelp residues and burkholderia pyrrocinia as raw materials - Google Patents

Preparation method and application of biofertilizer taking kelp residues and burkholderia pyrrocinia as raw materials Download PDF

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Publication number
CN110615719A
CN110615719A CN201911074554.5A CN201911074554A CN110615719A CN 110615719 A CN110615719 A CN 110615719A CN 201911074554 A CN201911074554 A CN 201911074554A CN 110615719 A CN110615719 A CN 110615719A
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China
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burkholderia pyrrocinia
residue
biofertilizer
kelp
kelp residue
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CN201911074554.5A
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Chinese (zh)
Inventor
王晓强
袁源
张成省
邹平
李义强
耿玉亭
周金辉
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Tobacco Research Institute of CAAS
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Tobacco Research Institute of CAAS
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A01C21/005—Following a specific plan, e.g. pattern
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Soil Sciences (AREA)
  • Toxicology (AREA)
  • Botany (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental & Geological Engineering (AREA)
  • Pest Control & Pesticides (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Fertilizers (AREA)

Abstract

The invention provides a preparation method of a biofertilizer taking kelp residues and burkholderia pyrrocinia as raw materials, belonging to the technical field of biofertilizer preparation and comprising the following steps: 1) mixing the kelp residue with water, performing microwave treatment, centrifuging the obtained microwave treatment product to obtain a first filter residue and a first clear liquid, centrifuging the first clear liquid to obtain a second clear liquid and a second filter residue, and freeze-drying the second clear liquid to obtain a kelp residue degradation product; 2) dissolving the degraded kelp residue obtained in the step 1) in water, inoculating Burkholderia pyrrocinia, and fermenting to obtain the biofertilizer. The invention takes kelp residue and Burkholderia pyrrocinia as raw materials to prepare the biological fertilizer, and the biological fertilizer can promote the growth of plants and can prevent and treat plant diseases.

Description

Preparation method and application of biofertilizer taking kelp residues and burkholderia pyrrocinia as raw materials
Technical Field
The invention belongs to the technical field of biological fertilizer preparation, and particularly relates to a preparation method and application of a biological fertilizer prepared from kelp residues and Burkholderia pyrrocinia.
Background
China is a traditional big agricultural country, and agriculture occupies a very important position in national production. The modern agricultural technology promotes the rapid development of agricultural production, and the popularization of chemical fertilizers enables the agricultural production to obtain great economic benefits. In recent years, the problems of environmental pollution, soil hardening, soil fertility decline, ecological deterioration and the like caused by using a large amount of fertilizers are increasingly serious, the environment is damaged, the soil fertility is influenced, and the quality of agricultural products is reduced. In order to realize sustainable development of agriculture and achieve the aims of high yield, high quality, ecology and safety, the development of economic and efficient natural fertilizers is of great significance.
Disclosure of Invention
In view of the above, the present invention aims to provide a preparation method and an application of a biofertilizer prepared from kelp residues and burkholderia pyrrocinia.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides a preparation method of a biofertilizer taking kelp residue and burkholderia pyrrocinia as raw materials, which comprises the following steps:
1) mixing the kelp residue with water, performing microwave treatment, centrifuging the obtained microwave treatment product to obtain a first filter residue and a first clear liquid, centrifuging the first clear liquid to obtain a second clear liquid and a second filter residue, and freeze-drying the second clear liquid to obtain a kelp residue degradation product;
2) dissolving the degraded kelp residue obtained in the step 1) in water, inoculating Burkholderia pyrrocinia, and fermenting to obtain the biofertilizer.
Preferably, the mass ratio of the kelp residue degradation product in the step 2) to water is (5-10) g to (0.1-2) L.
Preferably, the volume ratio of the mass of the kelp residue in the step 1) to the volume of water is (1-5) g to (10-50) ml.
Preferably, the microwave treatment conditions in step 1) include: the temperature of the microwave treatment is 150-200 ℃, the power of the microwave treatment is 800-1200W, and the time of the microwave treatment is 10-30 min.
Preferably, the centrifugation conditions of step 1) include: the rotating speed of the centrifugation is 9000-11000 rpm, and the time of the centrifugation is 15-25 min.
Preferably, the Burkholderia is inoculated with a Burkholderia pyrrocinia solution, and OD of the Burkholderia pyrrocinia solution600The value is 0.1 to 0.5.
Preferably, the volume ratio of the mass of the kelp residue degradation product to the Burkholderia pyrrocinia bacterial liquid is (5-10) g to (1-2) ml.
Preferably, the fermentation conditions of step 2) include: the fermentation time is 100-140 h, the fermentation temperature is 25-30 ℃, and the fermentation rotating speed is 150-200 rpm.
The invention also provides application of the biological fertilizer prepared by the preparation method in the technical scheme in promoting plant growth.
The invention also provides application of the biological fertilizer prepared by the preparation method in the technical scheme in plant disease control.
The invention provides a preparation method of a biofertilizer taking kelp residue and burkholderia pyrrocinia as raw materials, which comprises the following steps: 1) mixing the kelp residue with water, performing microwave treatment, centrifuging the obtained microwave treatment product to obtain a first filter residue and a first clear liquid, centrifuging the first clear liquid to obtain a second clear liquid and a second filter residue, and freeze-drying the second clear liquid to obtain a kelp residue degradation product; 2) dissolving the degraded kelp residue obtained in the step 1) in water, inoculating Burkholderia pyrrocinia, and fermenting to obtain the biofertilizer. The invention takes kelp residue and Burkholderia pyrrocinia as raw materials to prepare the biological fertilizer, and the biological fertilizer can promote the growth of plants and can prevent and treat plant diseases.
Drawings
FIG. 1 is the growth promotion result of the bio-fertilizer of example 1, wherein LJW + L is the bio-fertilizer prepared from kelp residue and Burkholderia pyrrocinia as raw materials, and NTC is the control;
FIG. 2 shows the results of preventing plant diseases in the biofertilizer of example 1, in which LJW + L is a biofertilizer prepared from kelp residue and Burkholderia pyrrocinia as raw materials, and NTC is a control.
Detailed Description
The invention provides a preparation method of a biofertilizer taking kelp residue and burkholderia pyrrocinia as raw materials, which comprises the following steps:
1) mixing the kelp residue with water, performing microwave treatment, centrifuging the obtained microwave treatment product to obtain a first filter residue and a first clear liquid, centrifuging the first clear liquid to obtain a second clear liquid and a second filter residue, and freeze-drying the second clear liquid to obtain a kelp residue degradation product;
2) dissolving the degraded kelp residue obtained in the step 1) in water, inoculating Burkholderia pyrrocinia, and fermenting to obtain the biofertilizer.
Mixing kelp residue with water, performing microwave treatment, centrifuging the obtained microwave treatment product to obtain a first filter residue and a first clear liquid, centrifuging the first clear liquid to obtain a second clear liquid and a second filter residue, and freeze-drying the second clear liquid to obtain a kelp residue degradation product.
In the invention, the kelp residue is preferably residue left after iodine and sodium alginate are extracted from kelp.
In the present invention, the ratio of the mass of the kelp residue to the volume of water is preferably (1 to 5) g to (10 to 50) ml, and more preferably (2 to 4) g to (20 to 40) ml.
In the present invention, the conditions of the microwave treatment preferably include: the temperature of the microwave treatment is preferably 150-200 ℃, and more preferably 160-180 ℃; the power of the microwave treatment is preferably 800-1200W, and more preferably 900-1100W; the time of the microwave treatment is preferably 10-30 min, and more preferably 15-25 min. The invention degrades the kelp residue through microwave treatment.
In the invention, the centrifugation is preferably carried out when the temperature of the microwave treatment object is 50-70 ℃, and the centrifugation conditions preferably comprise: the rotation speed of the centrifugation is preferably 9000-11000 rpm, and more preferably 10000 rpm; the time for centrifugation is preferably 15-25 min, and more preferably 20 min. In the present invention, the centrifugation removes solid matter of the kelp residue.
The time for freeze-drying the second clear liquid is not particularly limited, and the conventional freeze-drying condition is adopted. In the present invention, the obtained degraded kelp residue is preferably stored at-20 ℃.
The invention dissolves the degraded kelp residue in water, inoculates with Burkholderia pyrrocinia, and then ferments to obtain the biological fertilizer.
In the present invention, the Strain of Burkholderia pyrrocinia is preferably a Strain of Burkholderia pyrrocinia Lyc2 disclosed in the literature of Cloning and Analysis of Genes Controlling Antibacterial Activities of Burkholderia pyrrocinia Strain Lyc2. Xiaooqiang Wang et al Current Microbiology. In the present invention, the burkholderia pyrrocinia ferments the degradation product of the kelp residue.
In the present invention, the volume ratio of the mass of the degraded product of kelp residue to water is preferably (5 to 10) g to (0.1 to 2) L, more preferably (6 to 9) g to (0.8 to 1.5) L, and most preferably (7 to 8) g to (1 to 1.2) L.
In the present invention, the Burkholderia pyrrocinia is preferably inoculated with a Burkholderia pyrrocinia solution, and OD of the Burkholderia pyrrocinia solution600The value is preferably 0.1 to 0.5, more preferably 0.3. In the invention, the preparation of the burkholderia pyrrocinia bacterial liquid preferably comprises the following steps: inoculating Burkholderia pyrrocinia to LB liquid culture medium for culture, diluting the obtained culture solution, and measuring OD600The value is 0.1 to 0.5. The LB liquid medium is not particularly limited, and the present invention employsAnd (4) carrying out conventional treatment. In the invention, the culture temperature is preferably 25-30 ℃, and the culture time is preferably 20-30 h.
In the present invention, the conditions of the fermentation preferably include: the fermentation time is preferably 100-140 h, and more preferably 110-130 h; the fermentation temperature is preferably 25-30 ℃; the rotation speed of the fermentation is preferably 150-200 rpm, and more preferably 160-180 rpm.
In the invention, the biological fertilizer is a liquid fertilizer, the content of laminarin in the liquid fertilizer is 1-3 g/L, and the number of Burkholderia pyrrocinia is 1 multiplied by 1010～1011And (2) per liter.
The invention also provides application of the biological fertilizer prepared by the preparation method in the technical scheme in promoting plant growth.
The invention also provides application of the biological fertilizer prepared by the preparation method in the technical scheme in plant disease control.
In the present invention, the plant preferably includes pepper, tobacco, eggplant or cucumber.
In the present invention, the plant disease preferably includes a pepper bacterial wilt.
In the invention, the use method of the biological fertilizer is preferably 15-25 ml, preferably 20ml, of the biological fertilizer used for each plant, and the use method is root irrigation.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
1) Degradation of kelp residues: taking 2g of kelp residue, adding 20mL of water, degrading for 20min by using a microwave reactor (CEM Mars 6) under the conditions of 180 ℃ and 1000W of microwave power, centrifugally separating the extract when the reaction system is cooled to 60 ℃, and centrifugally treating the obtained extracting solution again to obtain the final extracting solution. Vacuum freeze drying the final extractive solution to obtain degraded product of herba Zosterae Marinae residue, and storing in refrigerator at-20 deg.C. Wherein the residue of herba Zosterae Marinae is residue obtained by extracting iodine and sodium alginate from herba Zosterae Marinae.
2) And (3) microbial fermentation: culturing Burkholderia pyrrocinia Lyc2 in LB culture medium for 24h, and diluting to OD600The value was 0.3, and a bacterial solution of Burkholderia pyrrocinia Lyc2 was obtained. Dissolving 5g of kelp residue degradation product in 1L of water, inoculating 1mL of Burkholderia pyrrocinia Lyc2 bacterial solution, and shake-culturing at 28 ℃ and 175rpm for 120h to prepare the kelp residue Burkholderia pyrrocinia Lyc2 functional liquid fertilizer.
Example 2
Transplanting pepper seedlings with consistent growth vigor into a sterilized matrix, and slowly filling the liquid fertilizer prepared in the example 1 into pepper rhizosphere 20ml per plant when the seedlings grow to two true leaves. The control group was watered with an equal volume of water. Irrigate every 5 days three times in succession. After the third watering for 7d, the growth promoting effect of the liquid fertilizer on the pepper was measured, and the results are shown in table 1 and fig. 1.
TABLE 1 growth promoting effect on Capsici fructus
Treatment of Plant height mm Number of blades mm Leaf length mm Leaf width mm
NTC 88.80±11.67 8.40±1.06 70.13±8.44 37.30±4.81
LJW+L 106.13±22.02 9.67±0.82 77.67±9.88 39.67±2.87
Note that: NTC-water blank; LJW-degradation products of kelp residue; L-Burkholderia pyrrocinia
As can be seen from Table 1 and FIG. 1, the functional fertilizer of Burkholderia pyrrocinia Lye2, which is a kelp residue, has a significant growth promoting effect on capsicum.
Example 3
After the pepper was transplanted, the liquid fertilizer prepared in example 1 was slowly poured around the roots of the pepper, 20ml per plant, and the control group was poured with an equal volume of water. Irrigate every 5 days three times in succession. Inoculating Laurella solanacearum around the rhizosphere of Capsici fructus 7 days after the third irrigation (the bacterium is added for allowing Capsici fructus to have bacterial wilt), and inoculating (OD) in the form of bacterial liquid6000.3), 20ml per strain, and the results of statistics of disease onset starting 5d after inoculation are shown in Table 2 and FIG. 2. The classification into 5 grades according to the classification standard of bacterial wilt disease proposed by Kempe (1983) and 0, 1, 2, 3 and 4.
Level 0: no symptoms of bacterial wilt;
level 1: 1-25% of leaves show wilting symptoms;
and 2, stage: the leaf withering symptom appears in 26-50% of leaves;
and 3, level: 51-75% of leaves have a wilting symptom;
4, level: 76-100% of leaves show wilting symptoms.
The disease index and the biocontrol effect are calculated according to the following formulas:
disease index is 100 × Σ (number of diseased plants × number of disease steps)/(total number of plants × number of highest disease steps);
relative prevention and treatment effect (%) (control disease index-treatment disease index)/control disease index × 100.
TABLE 2 statistics of the onset of pepper bacterial wilt
Note that: NTC-water blank; LJW-degradation products of kelp residue; L-Burkholderia pyrrocinia
As can be seen from the table 2 and the figure 2, the kelp residue Burkholderia pyrrocinia Lyc2 functional fertilizer has a remarkable prevention and treatment effect on the pepper bacterial wilt.
As can be seen from the above examples, the present invention prepares a biofertilizer using kelp residue and Burkholderia pyrrocinia as raw materials, the biofertilizer can promote the growth of plants and can control plant diseases.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (10)

1. A preparation method of a biofertilizer taking kelp residue and Burkholderia pyrrocinia as raw materials is characterized by comprising the following steps:
1) mixing the kelp residue with water, performing microwave treatment, centrifuging the obtained microwave treatment product to obtain a first filter residue and a first clear liquid, centrifuging the first clear liquid to obtain a second clear liquid and a second filter residue, and freeze-drying the second clear liquid to obtain a kelp residue degradation product;
2) dissolving the degraded kelp residue obtained in the step 1) in water, inoculating Burkholderia pyrrocinia, and fermenting to obtain the biofertilizer.
2. The method according to claim 1, wherein the mass-to-water volume ratio of the degraded kelp residue of step 2) is (5 to 10) g to (0.1 to 2) L.
3. The method according to claim 1, wherein the ratio of the mass of the kelp residue in the step 1) to the volume of water is (1 to 5) g to (10 to 50) ml.
4. The method according to claim 1, wherein the microwave treatment conditions of step 1) include: the temperature of the microwave treatment is 150-200 ℃, the power of the microwave treatment is 800-1200W, and the time of the microwave treatment is 10-30 min.
5. The method of claim 1, wherein the conditions for centrifugation in step 1) comprise: the rotating speed of the centrifugation is 9000-11000 rpm, and the time of the centrifugation is 15-25 min.
6. The method according to claim 1, wherein the Burkholderia pyrrocinia is inoculated with a Burkholderia pyrrocinia solution, and the OD of the Burkholderia pyrrocinia solution600The value is 0.1 to 0.5.
7. The method according to claim 1 or 6, wherein the ratio of the mass of the degraded product of kelp residue to the volume of the bacterial solution of Burkholderia pyrrocinia is (5-10) g to (1-2) ml.
8. The method according to claim 1, wherein the conditions for the fermentation in step 2) include: the fermentation time is 100-140 h, the fermentation temperature is 25-30 ℃, and the fermentation rotating speed is 150-200 rpm.
9. Use of the biofertilizer prepared by the preparation method according to any one of claims 1 to 8 for promoting plant growth.
10. Use of the biofertilizer prepared by the preparation method according to any one of claims 1 to 8 for controlling plant diseases.
CN201911074554.5A 2019-11-06 2019-11-06 Preparation method and application of biofertilizer taking kelp residues and burkholderia pyrrocinia as raw materials Pending CN110615719A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115350691A (en) * 2022-09-23 2022-11-18 浙江树人学院 Micro-plastic adsorbing material prepared from kelp fermentation residues, method and application
CN115992068A (en) * 2022-10-25 2023-04-21 上海市农业科学院 Burkholderia polyophaga and application thereof in promoting growth of edible fungi

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103304278A (en) * 2012-11-06 2013-09-18 青岛明月海藻集团有限公司 Seaweed fertilizer comprising seaweed leach liquor and preparation method thereof
KR101579485B1 (en) * 2015-06-12 2015-12-22 농업회사법인 이수농산 주식회사 Organic fertilizers and the preparation thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103304278A (en) * 2012-11-06 2013-09-18 青岛明月海藻集团有限公司 Seaweed fertilizer comprising seaweed leach liquor and preparation method thereof
KR101579485B1 (en) * 2015-06-12 2015-12-22 농업회사법인 이수농산 주식회사 Organic fertilizers and the preparation thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
马安民 等: "《园林植物杀虫剂应用技术》", 31 October 2017, 河南科学技术出版社 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115350691A (en) * 2022-09-23 2022-11-18 浙江树人学院 Micro-plastic adsorbing material prepared from kelp fermentation residues, method and application
CN115350691B (en) * 2022-09-23 2023-10-31 浙江树人学院 Micro-plastic adsorption material prepared from kelp fermentation residues, method and application
CN115992068A (en) * 2022-10-25 2023-04-21 上海市农业科学院 Burkholderia polyophaga and application thereof in promoting growth of edible fungi
CN115992068B (en) * 2022-10-25 2024-04-16 上海市农业科学院 Burkholderia polyophaga and application thereof in promoting growth of edible fungi

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