CN110615151B - Pasteurization filling equipment for filling bacillus agent and application thereof - Google Patents
Pasteurization filling equipment for filling bacillus agent and application thereof Download PDFInfo
- Publication number
- CN110615151B CN110615151B CN201911031543.9A CN201911031543A CN110615151B CN 110615151 B CN110615151 B CN 110615151B CN 201911031543 A CN201911031543 A CN 201911031543A CN 110615151 B CN110615151 B CN 110615151B
- Authority
- CN
- China
- Prior art keywords
- filling
- pipeline
- temperature
- bacillus agent
- pasteurization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65B—MACHINES, APPARATUS OR DEVICES FOR, OR METHODS OF, PACKAGING ARTICLES OR MATERIALS; UNPACKING
- B65B3/00—Packaging plastic material, semiliquids, liquids or mixed solids and liquids, in individual containers or receptacles, e.g. bags, sacks, boxes, cartons, cans, or jars
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65B—MACHINES, APPARATUS OR DEVICES FOR, OR METHODS OF, PACKAGING ARTICLES OR MATERIALS; UNPACKING
- B65B55/00—Preserving, protecting or purifying packages or package contents in association with packaging
- B65B55/02—Sterilising, e.g. of complete packages
- B65B55/12—Sterilising contents prior to, or during, packaging
- B65B55/14—Sterilising contents prior to, or during, packaging by heat
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65B—MACHINES, APPARATUS OR DEVICES FOR, OR METHODS OF, PACKAGING ARTICLES OR MATERIALS; UNPACKING
- B65B63/00—Auxiliary devices, not otherwise provided for, for operating on articles or materials to be packaged
- B65B63/08—Auxiliary devices, not otherwise provided for, for operating on articles or materials to be packaged for heating or cooling articles or materials to facilitate packaging
Abstract
The invention belongs to preparation of a microbial agent, and particularly relates to pasteurization filling equipment for filling a bacillus agent and application thereof. The pasteurization filling equipment for filling the bacillus agent comprises a pipeline and a valve which are communicated with a material output end at the bottom of a reaction kettle, and a distribution station arranged on the pipeline; the pipeline outside cladding that links to each other with the output of distribution station has the heating jacket that can carry out the heat exchange with the interior transported substance material of pipeline, sets up the heating jacket entry and the heating jacket export rather than the inner chamber intercommunication on the heating jacket, and the pipeline output end of heating jacket one side connects the liquid filling machine, and the material output of liquid filling machine connects the cold water pond. The invention effectively solves the problems of bottle expansion and bacterial agent blackening and stink generation caused by improper treatment in the production, storage and transportation processes of the existing microbial agent products, and has the advantages of small loss of viable bacteria in the production, packaging, storage and transportation processes, reduction of defective rate of products, production cost saving, improvement of product quality and grade and the like.
Description
Technical Field
The invention belongs to preparation of a microbial agent, and particularly relates to pasteurization filling equipment for filling a bacillus agent and application thereof.
Background
The bacillus is the main functional strain of microbial agent products. In the currently used strains ranked in the top ten of microbial agent products registered by the national ministry of agriculture, bacillus occupies 6 seats, and bacillus subtilis, paenibacillus mucilaginosus, bacillus megaterium, bacillus licheniformis and bacillus amyloliquefaciens occupy the vast majority.
The microbial fertilizer of microbial inoculum class is produced by fermentation, and mainly realizes the multiplication and expansion culture of functional strains. The process flow is as follows: slant preservation of tube strain → seed of Kirschner flask → seeding tank → fermentation product → liquid can → finished product. The selection of Bacillus as a functional strain of microbial agents is not separated from the excellent performance of Bacillus. Microbial agent products in which bacillus is a functional strain are generally preserved in a spore state. The main characteristics of spores are their strong resistance to high temperature, ultraviolet light, dryness, ionizing radiation and many toxic chemicals. The bacillus like bacteria agent product is less influenced by the external environment in the processes of packaging, storage, transportation and use. The product has long shelf life.
If the microbial inoculum product is improperly treated in the production, storage and transportation processes, the problems of bottle expansion and blackening and smelliness of the microbial inoculum are easily caused, and the commodity and the use effect of the microbial inoculum are influenced. In order to stabilize the product quality, production enterprises mostly adopt a method of adding preservatives such as sodium benzoate and sodium nitrite into a liquid microbial agent. The method can play a role in pollution prevention to a certain extent. However, these protective agents are all exogenous substances, the safety of the functional microbial strains and the applied crops and the environment for long-term application is to be examined, and the addition of any exogenous substance causes the increase of the cost.
The applicant considers that the problems of bottle rising, blackening and stink change of the microbial inoculum are caused by tracking sampling research for many years. Firstly, the fermentation of general bacillus often uses spore formation rate as fermentation index to carry out fermentation control, but the fermentation condition has a great influence on spore formation rate, and no 100% of thalli can form spores in actual production, so the thalli with weak activity is remained in the fermentation liquor after the fermentation is finished, and the life activity of the part of thalli in later period can influence product quality such as bottle expansion. Secondly, the vacuum packaging can not be realized by the existing filling process, partial air always remains in the bottle body of the packaged product, and strict aseptic operation can not be realized by most of the existing production enterprises due to the limitation of space and equipment during filling. During the filling process, microorganisms can easily enter the filling bottle body along with the air, mixed bacteria are easy to breed due to residual nutrient substances in microbial inoculum fermentation liquor, and a small amount of products can turn black and smelly within the quality guarantee period of the products, so that the production and the sale of the microbial inoculum are seriously influenced.
The pasteurization technology is a mature technology, is a low-temperature sterilization method invented by French microbiology pasteur, is a sterilization method which can kill germs and keep the flavor of nutrient substances in goods unchanged by utilizing lower temperature, and has various pasteurization procedures used at present. "Low temperature long time" and "high temperature short time" are two basic modes of operation. The low temperature is 62-65 ℃ in the ordinary operation for a long time, and the temperature is kept for 30 minutes, so that the method is an intermittent operation method. Compared with the method with low temperature for a long time, the operation method with high temperature and short time which is developed in recent years is more beneficial to industrial production and more popular in application.
The applicant does not find in the patent literature that there are similar patent literature reports to the present application.
Disclosure of Invention
The invention aims to provide pasteurization filling equipment for filling a bacillus agent and application thereof, which eliminate interference of mixed bacteria so as to achieve the purposes of prolonging the quality guarantee period of products and improving the use effect.
The overall technical concept of the invention is as follows:
the pasteurization filling equipment for filling the bacillus agent comprises a pipeline and a valve which are communicated with a material output end at the bottom of a reaction kettle, and a distribution station arranged on the pipeline; the pipeline outside cladding that links to each other with the output of distribution station has the heating jacket that can carry out the heat exchange with the interior transported substance material of pipeline, sets up the heating jacket entry and the heating jacket export rather than the inner chamber intercommunication on the heating jacket, and the pipeline output end of heating jacket one side connects the liquid filling machine, and the material output of liquid filling machine connects the cold water pond.
The application of pasteurization filling equipment for filling the bacillus agent in filling the bacillus agent.
The specific technical concept of the invention is as follows:
in order to detect the temperature of the materials in the pipeline in real time and meet the effect of killing the mixed bacteria, the preferred technical scheme is that a temperature detector capable of detecting the temperature of the materials conveyed in the pipeline is arranged at the output end of the pipeline on one side of the heating sleeve.
More preferably, the temperature detector is a thermometer or a temperature sensor. The applicant needs to say that the opening and closing and opening amount of the electromagnetic valve can be controlled by adopting a temperature sensor, a control device including but not limited to a single chip microcomputer, a PC and the like and an electromagnetic valve electric connection mode, and further the flow rate of the material and the working medium is controlled to achieve the purpose of automatic and accurate control.
In order to ensure the heat exchange effect between the working medium in the heating jacket and the bacillus agent in the pipeline and further effectively kill the mixed bacteria, the preferred technical scheme is that the length of the heating jacket is 2-4 meters.
In order to ensure the low-temperature treatment effect of the bacillus agent encapsulated in the closed container, the preferable technical scheme is that an inlet and an outlet which are communicated with the inner cavity of the cold water tank are arranged on the outer side of the cold water tank.
The application of the pasteurization filling equipment for filling the bacillus agent in filling the bacillus agent comprises the following process steps:
A. starting: injecting a working medium into the heating sleeve from an inlet of the heating sleeve, wherein the working medium is hot water or hot steam, opening a pipeline and a valve which are communicated with a material output end at the bottom of the reaction kettle, and adjusting the valve of the distribution station;
B. temperature adjustment: according to the temperature displayed by the temperature detector, adjusting the working medium and the flow rate of the bacillus agent in the pipeline to keep the temperature of the bacillus agent in the pipeline detected by the temperature detector at 75-80 ℃;
C. filling: filling the bacillus agent with the pipeline temperature of 75-80 ℃ into the container and sealing the opening of the container;
D. cooling: and (3) placing the container which is internally provided with the bacillus agent and has a sealed container opening in a cold water pool for keeping for 5-10 minutes, wherein the cold water pool is filled with cooling water.
The applicant needs to explain that the working medium is added according to the sequence of 'steam is fed in and discharged out from top to bottom, and water is fed in and discharged out from top to bottom'; the applicant is not further described herein because it belongs to the conventional art.
More preferably, in the step D, the cooling water circulates through the water cooling tower through an inlet and an outlet on the outer side of the cold water pool.
The applicant needs to state that:
in the description of the present invention, the terms "output", "bottom", "inlet", "outlet", "end", "side", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of simplifying the description of the present invention, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
To verify the technical effect of the present invention, the applicant conducted the following tests:
the bacterial content of the functional strains is an important index for measuring the quality of the microbial agent, and the standard of the viable count of various types of microbial agents is clearly specified in agricultural microbial agents (GB 20287-2006). Therefore, the number of viable bacteria in the bacteria liquid is the important factor in the work in the processes of fermentation, dumping and packaging of the microbial agent.
First, test method
The tests were carried out on two packaging lines in the same packaging plant. One is the original packaging production line; one is a packaging production line which is transformed by a new process, and other conditions are the same. The test simply compares the same batch of fermentation liquor filled by the process and the original process, and randomly extracts 5 batches of products on different production lines for storage test. And (4) inspecting the influence of different processes on the viable count of the product and the bacterial contamination rate of the product.
Second, test results
1. Influence of new and old packaging processes on viable count of microbial agent (plate coating method)
TABLE 1 Effect of different processes on the viable count of the product (unit: hundred million/mL)
| Time (d) | 0 | 7 | 14 | 30 | 60 | 90 | 180 | 360 |
| Control group | 5.68 | 5.65 | 5.65 | 5.36 | 5.28 | 5.20 | 5.18 | 5.09 |
| Test group | 5.63 | 5.63 | 5.61 | 5.38 | 5.30 | 5.21 | 5.17 | 5.18 |
Note: the test results are the average of 5 batches.
As can be seen from Table 1, the lower-line bacteria content of the control group and the test group is 5.63 hundred million/ml and 5.68 hundred million/ml respectively, which is within the error range of the test without difference, and the viable count of the fermentation liquor of the product of the test group after being heated and cooled by the process of the invention is basically not influenced. The loss rate of viable bacteria of the product of the control group after being stored for one year is 11.6 percent, and the loss rate of viable bacteria of the product of the test group after being stored for one year is 8.0 percent and is 3.6 percent lower than that of the control group. Although the microbial inoculum is allowed to lose about 10 percent in the whole packaging, storing and transporting process according to a great deal of previous related tracking detection and industry experience of the applicant, the loss of viable bacteria is required to be reduced as much as possible in the processes of production, packaging, storing and transporting, and the process has obvious advancement.
2. Influence of new and old packaging processes on product contamination rate
TABLE 2 influence of different processes on the contamination rate of the product
| Time (d) | 7 | 14 | 30 | 60 | 90 | 180 | 360 |
| Control group (%) | 0 | 2.45 | 3.95 | 4.40 | 4.25 | 4.25 | 4.28 |
| Test group (%) | 0 | 1.05 | 1.15 | 1.22 | 1.22 | 1.22 | 1.21 |
Note: the test results are the average of 5 batches.
The results in table 2 show that the annual rate of contamination of the products preserved by the process of the invention is 1.21%, while the rate of contamination of the products filled by the old process reaches 4.28%, and the rate of contamination is reduced by 3.07% by the filling process of the invention. The method has practical significance in actual production, can reduce the defective rate of products, saves the production cost and improves the quality and the grade of the products.
The invention has the substantive characteristics and the obvious technical progress that:
the invention introduces pasteurization technology on the liquid filling and packaging process of the microbial inoculum, eliminates a part of thalli with weaker activity; the vacuum degree in the packing material is ensured, and the interference of mixed bacteria is eliminated, so that the aims of prolonging the quality guarantee period of the product and improving the use effect are fulfilled. The applicant tests prove that the process has small viable bacteria loss in the processes of production, packaging, storage and transportation, can reduce the defective rate of products, save the production cost and improve the quality and the grade of the products.
Drawings
FIG. 1 is a process flow diagram of the present invention.
The reference numbers in the drawings are as follows:
1. a reaction kettle; 2. a distribution station; 3. an outlet of the heating jacket; 4. heating a jacket; 5. an inlet of the heating jacket; 6. a thermometer or temperature sensor; 7. filling machine; 8. a cold water pool.
Detailed Description
The present invention is further described with reference to the following examples, which should not be construed as limiting the scope of the present invention, but the scope of the present invention is defined by the appended claims, and any equivalent technical means may be substituted according to the present specification without departing from the scope of the present invention.
The overall structure in the embodiment is shown in the figure, and comprises a pipeline and a valve which are communicated with a material output end at the bottom of the reaction kettle 1, and a distribution station 2 arranged on the pipeline; the pipeline outside that links to each other with the output of distribution station 2 cladding has the heating jacket 4 that can carry out the heat exchange with the interior transported substance material of pipeline, sets up heating jacket entry 5 and heating jacket export 3 rather than the inner chamber intercommunication on the heating jacket 4, and the pipeline output end-to-end connection liquid filling machine 7 of heating jacket 4 one side, the material output of liquid filling machine 7 connects cold water pool 8.
The output end of the pipeline at one side of the heating jacket 4 is provided with a temperature detector which can detect the temperature of the materials conveyed in the pipeline.
The temperature detector is a thermometer or a temperature sensor 6.
The length of the heating jacket 4 is 2-4 meters.
The outer side of the cold water pool 8 is provided with an inlet and an outlet which are communicated with the inner cavity of the cold water pool.
The application of the pasteurization filling equipment for filling the bacillus agent in filling the bacillus agent comprises the following process steps:
A. starting: injecting a working medium into the heating jacket 4 from the heating jacket inlet 5, wherein the working medium is hot water or hot steam; opening a pipeline and a valve communicated with a material output end at the bottom of the reaction kettle 1, and adjusting the valve of the distribution station 2;
B. temperature adjustment: according to the temperature displayed by the temperature detector, adjusting the working medium and the flow rate of the bacillus agent in the pipeline to keep the temperature of the bacillus agent in the pipeline detected by the temperature detector at 75-80 ℃;
C. filling: filling the bacillus agent with the pipeline temperature of 75-80 ℃ into the container and sealing the opening of the container;
D. cooling: and (3) placing the container which is internally provided with the bacillus agent and has a sealed container opening in a cold water pool 8 for 5-10 minutes, wherein the cold water pool 8 is filled with cooling water.
And D, circulating the cooling water in the step D through the water cooling tower through an inlet and an outlet on the outer side of the cold water pool 8.
Claims (5)
1. The pasteurization filling equipment for filling the bacillus agent comprises a pipeline and a valve which are communicated with a material output end at the bottom of a reaction kettle (1), and a distribution station (2) arranged on the pipeline; the automatic temperature control device is characterized in that the outer side of a pipeline connected with the output end of the distribution station (2) is coated with a heating sleeve (4) capable of exchanging heat with conveyed materials in the pipeline, the heating sleeve (4) is provided with a heating sleeve inlet (5) and a heating sleeve outlet (3) communicated with the inner cavity of the heating sleeve, the pipeline output end of one side of the heating sleeve (4) is connected with a filling machine (7), the pipeline output end of one side of the heating sleeve (4) is provided with a temperature detector capable of detecting the temperature of the conveyed materials in the pipeline, the material output of the filling machine (7) is connected with a cold water tank (8), and the outer side of the cold water tank (8) is provided with an inlet and an outlet communicated with the inner cavity of the cold water tank.
2. The pasteurization filling apparatus for filling with a bacillus agent as claimed in claim 1, wherein the temperature detector is selected from a thermometer or a temperature sensor (6).
3. The pasteurization filling plant for filling with a bacillus agent as claimed in claim 1, characterized in that the heating jacket (4) has a length of 2-4 m.
4. The application of the pasteurization filling equipment for filling the bacillus agent as claimed in claims 1-3 in filling the bacillus agent is characterized by comprising the following process steps:
A. starting: injecting a working medium into the heating sleeve (4) from the heating sleeve inlet (5), wherein the working medium is hot water, opening a pipeline and a valve which are communicated with a material output end at the bottom of the reaction kettle (1), and adjusting the valve of the distribution station (2);
B. temperature adjustment: according to the temperature displayed by the temperature detector, adjusting the working medium and the flow rate of the bacillus agent in the pipeline to keep the temperature of the bacillus agent in the pipeline detected by the temperature detector at 75-80 ℃;
C. filling: filling the bacillus agent with the pipeline temperature of 75-80 ℃ into the container and sealing the opening of the container;
D. cooling: the container with the bacillus agent inside and the sealed container opening is placed in a cold water pool (8) for 5-10 minutes, and cooling water is filled in the cold water pool (8).
5. The use according to claim 4, characterized in that in step D the cooling water is circulated through the water cooling tower via the inlet and outlet outside the cold water basin (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911031543.9A CN110615151B (en) | 2019-10-28 | 2019-10-28 | Pasteurization filling equipment for filling bacillus agent and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911031543.9A CN110615151B (en) | 2019-10-28 | 2019-10-28 | Pasteurization filling equipment for filling bacillus agent and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110615151A CN110615151A (en) | 2019-12-27 |
| CN110615151B true CN110615151B (en) | 2021-10-08 |
Family
ID=68926773
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911031543.9A Active CN110615151B (en) | 2019-10-28 | 2019-10-28 | Pasteurization filling equipment for filling bacillus agent and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110615151B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3899862A (en) * | 1971-04-06 | 1975-08-19 | Lever Brothers Ltd | Sterilization of containers |
| CN1148936A (en) * | 1996-09-02 | 1997-05-07 | 中国科学院生态环境研究中心 | Concentration of Thuricide |
| CN104478600A (en) * | 2014-12-18 | 2015-04-01 | 山东大地生物科技有限公司 | Functional microbial agent and preparation method thereof |
| CN104522163A (en) * | 2009-03-27 | 2015-04-22 | Gea农场技术股份有限公司 | Apparatus and method for pasteurizing milk for feeding to calves |
| CN204377840U (en) * | 2014-12-31 | 2015-06-10 | 海南达川食品有限公司 | Pasteurisation apparatus |
| CN209286249U (en) * | 2018-06-23 | 2019-08-23 | 山东住商红福农业发展股份有限公司 | Bactericidal unit is used in a kind of production of microbial bacterial agent |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204433178U (en) * | 2014-12-30 | 2015-07-01 | 上海沃迪自动化装备股份有限公司 | A kind of dairy beverage miniature sterilizing homogeneous bulking system |
-
2019
- 2019-10-28 CN CN201911031543.9A patent/CN110615151B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3899862A (en) * | 1971-04-06 | 1975-08-19 | Lever Brothers Ltd | Sterilization of containers |
| CN1148936A (en) * | 1996-09-02 | 1997-05-07 | 中国科学院生态环境研究中心 | Concentration of Thuricide |
| CN104522163A (en) * | 2009-03-27 | 2015-04-22 | Gea农场技术股份有限公司 | Apparatus and method for pasteurizing milk for feeding to calves |
| CN104478600A (en) * | 2014-12-18 | 2015-04-01 | 山东大地生物科技有限公司 | Functional microbial agent and preparation method thereof |
| CN204377840U (en) * | 2014-12-31 | 2015-06-10 | 海南达川食品有限公司 | Pasteurisation apparatus |
| CN209286249U (en) * | 2018-06-23 | 2019-08-23 | 山东住商红福农业发展股份有限公司 | Bactericidal unit is used in a kind of production of microbial bacterial agent |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110615151A (en) | 2019-12-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2174361C2 (en) | Method of sterilizing nonmilk low-acidity product | |
| Somavat et al. | Accelerated inactivation of Geobacillus stearothermophilus spores by ohmic heating | |
| Goverd et al. | The occurrence and survival of coliforms and salmonellas in apple juice and cider | |
| Garcia-Gonzalez et al. | Inactivation of naturally occurring microorganisms in liquid whole egg using high pressure carbon dioxide processing as an alternative to heat pasteurization | |
| CN105087361A (en) | Suspension type biological indicator and preparation method thereof | |
| CN108476867A (en) | The sterilizing cooling inoculation Integration Equipment of culture medium of edible fungus | |
| CN204092635U (en) | Culturing edible fungus solid medium microwave sterilizer | |
| CN110615151B (en) | Pasteurization filling equipment for filling bacillus agent and application thereof | |
| CN113460403B (en) | System and method for aseptic canning sterilization of solid food products | |
| CN111808787A (en) | Method for improving germination rate of spores of clostridium sporogenes | |
| Dziugan et al. | Ozonation as an effective way to stabilize new kinds of fermentation media used in biotechnological production of liquid fuel additives | |
| CN101121919A (en) | Method for long conserving microorganism bacterial | |
| CN101518356B (en) | Method for sterilizing pollen | |
| CN207276611U (en) | A kind of methanol effective sterilizing system of high density fermentation | |
| Thornley | Microbiological aspects of the use of radiation for the elimination of Salmonellae from foods and feeding stuffs | |
| CN110438060A (en) | A kind of preparation of Geobacillus stearothermophilus gemma, enrichment method | |
| CN108359706A (en) | A kind of culture medium and preparation method thereof for quickly detecting the lactobacillus of resistance to acetic acid | |
| CN107125298A (en) | A kind of controlled atmospheric packing section boiled salted duck low-temp antistaling method | |
| US20110030567A1 (en) | System for transporting and/or washing and/or pasteurisation thermal treatment of foodstuffs, particularly leaf products | |
| CN102838416A (en) | Liquid biological potassium fertilizer protecting agent | |
| CN101496626A (en) | Method of sterilizing liquid and liquid food | |
| CN101824380A (en) | Production method of fungus test bottle | |
| Gabriel et al. | Thermal death times of Escherichia coli in young coconut endosperm beverage | |
| JP3805600B2 (en) | Complete sterilization method and food sterilization method using the same | |
| US20060053850A1 (en) | Package for storing a formulate liquid inoculating solution containing rhizobia and process for growing rhizobia in it |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: No. 2089, Wusi Middle Road, Baoding City, Hebei Province 071051 Patentee after: Hebei Institute of Microbiology Co.,Ltd. Address before: No. 2089, Wusi Middle Road, Baoding City, Hebei Province 071051 Patentee before: HEBEI Research Institute OF MICROBIOLOGY |