CN110607295A

CN110607295A - Preparation process and preparation method for extracting feed-grade pancreatin from pancreatin residues

Info

Publication number: CN110607295A
Application number: CN201910599846.4A
Authority: CN
Inventors: 蔡青和; 朱琳娜
Original assignee: SHANGHAI HONEST BIOLOGICAL TECHNOLOGY Co Ltd
Current assignee: SHANGHAI HONEST BIOLOGICAL TECHNOLOGY Co Ltd
Priority date: 2019-07-04
Filing date: 2019-07-04
Publication date: 2019-12-24

Abstract

The invention belongs to the technical field of pancreatin extraction, and particularly relates to a preparation process for extracting feed-grade pancreatin from pancreatin residues, which comprises the following steps: the method comprises the following steps: raw material treatment: the raw material is frozen pancreas residue obtained after extracting angiotensin from crude enzyme powder, the raw material is dissolved by distilled water, the raw material is crushed by a flat plate crusher or a flaker, and then is stirred by a stirrer for 15-30 minutes to be dissolved, or is ground and dissolved by a pulping machine; step two: centrifuging: centrifuging with a centrifuge or filtering with a separator to remove residue to obtain supernatant filtrate; step three: and (3) precipitation and concentration: the invention has the characteristics of small pollution, simple equipment, short production period, no need of adding an activating agent, stable property and convenient storage after adding 60-100% ethanol into the supernatant filtrate and uniformly stirring and then precipitating, and can obviously improve the production performance of animals or improve the utilization rate of feed raw materials when being added into feed for feeding livestock and poultry.

Description

Preparation process and preparation method for extracting feed-grade pancreatin from pancreatin residues

Technical Field

The invention relates to the technical field of pancreatin extraction, in particular to a preparation process for extracting feed-grade pancreatin from the residual pancreatin slag after extracting angiotensin from crude enzyme powder and a preparation method thereof.

Background

The digestive tract of young animals such as piglets, chicks, ducklings, dairy cows and the like is underdeveloped, an endogenous digestive enzyme system is not established, and the young animals are easy to have nutritional diarrhea and diarrhea caused by other reasons due to early weaning and other feeding systems. By adding suitable enzyme preparation, the occurrence of nutritional diarrhea in young animals can be prevented, thereby reducing the use of antibiotics.

Pancreatin, a protein with special functions. Pancreatin in the pharmaceutical industry is mainly used for dyspepsia, inappetence and digestive disorder caused by liver and pancreas diseases, and is also suitable for congenital pancreatic insufficiency, and pancreatic insufficiency caused by abdominal operation and traumatic pancreatic resection. Or pancreatic insufficiency caused by non-surgical excision and chronic pancreatitis caused by alcoholism. Also, pancreatin has a wide range of applications in the feed industry, not only providing a protein source to animals, but also because it contains a large number of biologically active components, such as proteases, amylases and lipases and active peptides.

At present, the traditional preparation process of industrial pancreatin mostly takes pig or ox pancreas as a raw material, and comprises a plurality of procedures of raw material treatment (flaking, grinding into thick liquid), activation, extraction, separation (filtration, precipitation, squeezing), degreasing, granulation, drying, crushing and the like. The raw material for extracting angiotensin for medicine is derived from crude enzyme powder (derived from pig pancreas), and the crude enzyme powder contains angiotensin, trypsin, pancreatic amylase and pancreatic lipase. The process for extracting the angiotensin mainly adopts water washing and specific molecular filter membrane filtration, and the enzyme in the angiotensin is slightly damaged. The pancreatic residues left after the crude enzyme powder extracts the angiotensin for medicine are used as raw materials for preparing the pancreatin, so that the economic value and the feasibility are certain, on one hand, the use of a large amount of organic solvents and the addition of protective agents are avoided, on the other hand, the pancreatic resources are fully and comprehensively utilized, and the method has great significance in the aspects of environmental protection and resource saving. At present, the process for preparing pancreatin by using the pancreatic residues left after the extraction of angiotensin for medicine is not reported yet. Taking the rest pancreas residue after extracting insulin from pancreas as raw material, directly precipitating with PEG-6000 and passing through CaCl₂Activated pancreatic residues and supernatant are subjected to the procedures of centrifugation, acetone precipitation, ether degreasing and the like to prepare pancreatin, but acetone, ether and other dangerous chemicals which are easy to prepare poison and explode are still used in the method, the control difficulty under the production environment of a factory is increased, and PEG-6000 viscosity is highThe operation difficulty of centrifugation, filtration and the like is increased, and the product also carries more PEG, which needs further treatment, so that the development of a preparation process for extracting the feed-grade pancreatin from the pancreatin residue is urgently needed.

Disclosure of Invention

This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. In this section, as well as in the abstract and the title of the invention of this application, simplifications or omissions may be made to avoid obscuring the purpose of the section, the abstract and the title, and such simplifications or omissions are not intended to limit the scope of the invention.

The present invention has been made in view of the above and/or problems occurring in the existing process for preparing feed-grade pancreatin extracted from pancreatic residues.

Therefore, the invention aims to provide a preparation process for extracting feed-grade pancreatin from pancreatin residues, which has the advantages of small pollution, simple equipment, short production period, no need of adding an activating agent, stable property and convenient storage, and can obviously improve the production performance of animals or improve the utilization rate of feed raw materials when added into feed for feeding livestock and poultry.

To solve the above technical problem, according to an aspect of the present invention, the present invention provides the following technical solutions:

a preparation process for extracting feed-grade pancreatin from pancreatin residues comprises the following steps:

the method comprises the following steps: raw material treatment: the raw material is frozen pancreas residue obtained after extracting angiotensin from crude enzyme powder, the raw material is crushed by a flat plate crusher or a flaker, dissolved by distilled water and stirred by a stirrer for 15-30 minutes, or ground and dissolved by a pulping machine;

step two: centrifuging: centrifuging with a centrifuge or filtering with a separator to remove residue to obtain supernatant filtrate;

step three: and (3) precipitation and concentration: adding 60% -100% ethanol into the supernatant filtrate, stirring, precipitating at 10-20 deg.C for 0.5-2.5 hr, removing supernatant, and collecting precipitate;

step four: drying and granulating: mixing the precipitate obtained in the third step with a carrier for feed, and drying by using a drying device or drying after granulating to obtain pancreatin;

step five: detecting and packaging: the pancreatin comprises trypsin, pancrelipase and pancreatin, the trypsin is more than or equal to 1000u/g, the pancreatin is more than or equal to 500u/g, the activity of the pancrelipase is more than or equal to 800u/g, the water content is less than or equal to 6 percent, the storage period is more than or equal to 12 months, and the pancreatin is packaged after being detected to be qualified.

As a preferable scheme of the preparation process for extracting the feed-grade pancreatin from the pancreatin residue, the invention comprises the following steps: the carrier for the feed is any one of corn starch, silicon dioxide, gelatin and rice hull powder.

As a preferable scheme of the preparation process for extracting the feed-grade pancreatin from the pancreatin residue, the invention comprises the following steps: the drying device is air-blast drying, vacuum drying or fluidized bed drying.

As a preferable scheme of the preparation process for extracting the feed-grade pancreatin from the pancreatin residue, the invention comprises the following steps: the dissolving temperature is controlled at 10-20 ℃, and the ratio of distilled water to raw materials is controlled at 3-1: 1.

Compared with the prior art: the preparation process for extracting the feed-grade pancreatin from the pancreatic residues has the advantages of small pollution, simple equipment, short production period, no need of adding an activating agent, stable property and convenient storage, and can obviously improve the production performance of animals or improve the utilization rate of feed raw materials and increase the apparent digestibility of nutrient substances without influencing the content of blood metabolites and bacteria in excrement when being added into the feed for feeding livestock and poultry.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the present invention will be described in detail below with reference to the accompanying drawings and detailed embodiments, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive exercise. Wherein:

FIG. 1 is a flow chart of the production process for extracting feed-grade pancreatin from pancreatin dregs.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described herein, and it will be apparent to those of ordinary skill in the art that the present invention may be practiced without departing from the spirit and scope of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.

Embodiment mode 1

1. Preparation of

The preparation process for extracting the feed-grade pancreatin from the pancreatin residue comprises the following steps:

the method comprises the following steps: raw material treatment: the raw material is frozen pancreas residue obtained after extracting angiotensin from crude enzyme powder, and is pulverized by a flat plate pulverizer or a flaker, dissolved by distilled water, the dissolution temperature is controlled at 10-20 ℃, the ratio of distilled water to the raw material is controlled at 3-1:1, and then stirred by a stirrer for 15-30 minutes for dissolution, or ground and dissolved by a pulping machine;

step two: centrifuging: centrifuging by a centrifugal machine or filtering and removing slag by the centrifugal machine to obtain supernatant filtrate;

step three: and (3) precipitation and concentration: adding 60-100% ethanol into the supernatant, stirring, precipitating at 10-20 deg.C for 0.5-2.5 hr, removing supernatant, and collecting precipitate;

step four: drying and granulating: mixing the precipitate with appropriate amount of carrier for feed such as corn starch, silicon dioxide, rice hull powder, etc., drying or granulating and drying; the drying can be selected from forced air drying, vacuum drying or fluidized bed drying;

2. And (3) detection: refer to the measurement method in the second part of the 2010 version of Chinese pharmacopoeia.

3. The product quality indexes are as follows:

(1) the water content is 5.37%

(2) Trypsin activity 1326u/g

(3) Pancreatic amylase activity 612u/g

(4) Pancreatic lipase activity 873u/g

Embodiment mode 2

1. Preparation of

the method comprises the following steps: raw material treatment: the raw material is frozen pancreatic residue obtained after extracting angiotensin from crude enzyme powder, pulverizing with a plate pulverizer, dissolving with distilled water at 10 deg.C, and controlling the ratio of distilled water to raw material at 2: 1;

step two: centrifuging: centrifuging at 2500rpm for 5 min to obtain supernatant;

step three: and (3) precipitation and concentration: adding 75% ethanol into the supernatant filtrate, stirring, precipitating at 10 deg.C for 1 hr, removing supernatant, and collecting precipitate;

step four: drying and granulating: mixing the precipitate with appropriate amount of corn starch, gelatin, etc., stirring, and fluidized bed drying to obtain powder;

3. The product quality indexes are as follows:

(1) the water content is 5.20%

(2) Trypsin activity 1137u/g

(3) Activity of amylopsin 635u/g

(4) Pancreatic lipase activity 963u/g

First, pancreatin animal test effect verification

Purpose of the test

Different doses of pancreatin are added into corn-soybean meal type daily ration of 1-38-day-old broiler chickens, the influence of the pancreatin on the growth performance of the broiler chickens is measured, and the application effect of the pancreatin on the broiler chickens is comprehensively evaluated.

2 materials and methods

2.1 test materials

144 healthy and consistent weight AA broiler chickens of 1 day of age were used, and the pancreatin product was provided by Shanghai Europe and resistant Biotechnology Limited.

2.2 test design

Healthy 1-day-old AA broiler chickens 144 with consistent weight (the weight variation is 12%, the target weight is 42 +/-5 g, and the actual average weight depends on the hatching mass) are selected and randomly divided into 4 treatments, wherein each treatment is 6 times, and each treatment is 6 times. The specific test design is shown in table 1.

TABLE 1 design of the experiments

Note: no antibiotics are added into the daily ration, and the addition amount of the phytase is 200g/T (10000IU/g)

2.3 test daily ration

The experimental basic daily ration is prepared into corn-soybean meal type powder by referring to the Anweijie AA commercial broiler chicken feeding and the content standard of the nutritional ingredients of the commercial material, and the nutritional index setting refers to the following table 2.

TABLE 2 Nutrition index set points

2.4 Breeding management and detection indexes

2.4.1 Breeding management

Before raising, the chicken coop is fumigated and disinfected by potassium permanganate, and a stockline waterline is cleaned. The patients can eat and drink water freely in the whole test period. Keeping the chicken house ventilated and clean, and cleaning and disinfecting manure every week. The immunization process is carried out according to the conventional immunization. The natural illumination of the henhouse is supplemented with artificial light, the henhouse temperature is controlled at 33-35 ℃ in the 1 st week, and then is reduced by 2-3 ℃ every week, and is gradually reduced to 25 ℃ in the 4 th week. The test period is 1-14d, 15-28d and 29-38 d.

2.4.2 index determination

The weights were weighed at 1, 14, 24, and 42 days of age, respectively, and the average body weight and daily gain were calculated. 14. 24, 42 daily ration are nodulated, and Average Daily Gain (ADG), Average Daily Feed Intake (ADFI) and feed-weight ratio (F/G) are calculated. In order to accurately calculate the feed intake, the thrown materials need to be collected and weighed regularly. The number of dead chickens was recorded and the mortality was calculated.

2.4.3 data processing and statistical analysis method

The data are summarized by an Excel table, the data are subjected to one-factor variance analysis by PASW18.0 software, and multiple comparison is performed by a Duncan method, wherein P <0.05 indicates that the difference is obvious, and P <0.05 indicates that the difference is obvious.

3 results and analysis

The influence of adding pancreatin to the daily ration on the growth performance of the broiler chickens within 1-14d, 15-28d, 29-38d and the whole AA is shown in table 3.

The addition of pancreatin in the daily ration of the broiler chickens of 1-14 days has no obvious influence on daily weight gain, but the average daily feed intake and feed conversion ratio of the broiler chickens are reduced.

The addition of 150-500g/t of pancreatin to the daily ration of the broilers in 15-28 days can improve the average daily gain of the broilers and obviously reduce the feed-meat ratio (P < 0.05).

The average daily gain of the broiler chickens can be improved by adding 500g/t of pancreatin 150-t in the daily ration of the broiler chickens within 29-38d, and the feed-meat ratio of the broiler chickens is obviously reduced when the addition amount is 500g/t (P is less than 0.05).

Through comprehensive growth whole-process analysis, the average daily gain of the broilers can be improved by adding 500g/t of pancreatin 150-.

TABLE 3 influence of pancreatin addition to the diet on the productivity of white feather broilers at various growth stages

Second, pancreatin animal test effect verification

Purpose of the test

Feeding tests are carried out for 42 days under actual production conditions, and the influence of the pancreatin product on the production performance and economic benefit of broiler chickens is comprehensively evaluated by collecting broiler chicken production performance data.

2 materials and methods

2.1 test materials

Pancreatin, supplied by Shanghai Europe Stationery Biotechnology Ltd. The main effective components are as follows: trypsin, pancreatic lipase, pancreatic amylase and enzyme activity detection method refer to Chinese pharmacopoeia.

Test animals: 3000 healthy broiler chicks of 0 day old and similar weight.

2.2 test design and handling

3000 healthy broiler chicks of 0 day old and with consistent weight are selected and randomly divided into 2 treatments, each treatment is 10 times repeated, and each treatment is 150 times repeated. The specific test design is shown in table 4.

Table 4 experimental design

2.3 test daily ration

The test adopts corn and soybean meal type basic ration, wherein the material type of the ration is granular material in the early stage, and the material type of the ration is powder in the middle and later stages. The test refers to the group standard of daily ration protein and amino acid level and the actual preparation of combined production, and refers to Table 5 for the sectional daily ration and partial nutrition index level.

TABLE 5 control of the segmental and partial nutritional indicators of the diets

2.4 Breeding management

The experiment unifies the feeding management and the immunization program. During the test, the feed was manually fed, and the food and water were taken freely.

2.5 data Collection

During the test, the cumulative feed intake at 7 days, 14 days, 21 days, 35 days and 42 days was recorded in the unit of repetition and used to calculate the average daily feed intake at each stage. The total weight of the sample is weighed in a repetition unit at 7 days of age and 42 days of age, and the sample is weighed at 15 percent of 21 days of age for calculating the average weight and the feed-meat ratio.

During the test, the health of the chicken flocks was observed every day, and the number of dead chickens was recorded in duplicate and weighed for calculating the dead panning rate and corrected feed-meat ratio of dead chickens.

And (4) after the test is finished, calculating the Ouyi index and the gross profit by taking the repetition as a unit for evaluating the economic benefit.

2.6 statistical analysis

The data were summarized using Excel tables and counted using SPSS Statistics.

3. Test results

As is clear from Table 6, the average body weight differences at 7-day, 21-day and 42-day-old of each test group were not significant (P >0.05), and the feed-meat ratio at 0-21-day-old (P <0.05) was significantly decreased in the group to which 250 g/t of pancreatin was added, and the feed-meat ratio at 0-42-day-old tended to decrease, as compared with the control group. The group with 250 g/ton of pancreatin has the tendency of improving the Ouyi index and only the Maoli.

TABLE 6 influence of pancreatin on broiler productivity and economic benefits

Remarking: 1. the feed-meat ratio (without dead panning) was calculated as feed consumption/(end weight-0 day old chick weight).

2. The data in the same row are marked with different lower case letters to indicate that the difference is significant (P <0.05), and the same letter or no letter to indicate that the difference is not significant (P > 0.05).

Third, pancreatin animal test effect verification

Purpose of the test

The test evaluates the influence of the pancreatin added into sorghum bean pulp type daily ration (SSBM) on the growth performance, the apparent digestibility of nutrient substances and the content of fecal flora of growing-finishing pigs.

2 materials and methods

2.1 test animals and rearing management

80 healthy (Du X long X big) 67-day-old growing pigs were selected for the trial period of 70 days. Pancreatin is supplied by Shanghai Europe Stadium Biotechnology Limited. Sorghum is imported from Tianjin harbor, and corn is common yellow corn used as feed in Fengning test base.

The test was carried out in 2016 at the experimental research base of Hebei Fengning pigs, university of agriculture, China, from 4 to 8 months. The equipment and conditions of each pigsty were identical. The ground is cement ground, the average temperature and humidity of the colony house are respectively 28 ℃ and 50%, and other management is carried out according to a conventional method. Two-stage breeding, namely 25-50kg and 50-75 kg.

2.2 test design and daily ration

80 healthy growing pigs of similar initial body weight (23.4 + -3.5 kg) were divided into 4 treatments by a fully randomized block of body weight and gender, 5 replicates (pens) per treatment, 4 pigs per replicate, and half of the sire and the mother.

The control diet is corn soybean meal type diet (CSBM) with corn content of about 70%. Sorghum soybean meal type ration (SSBM): during the pre-trial period (d 0-35), 50% of the corn was replaced by 50% sorghum; in the later period of the test (d 36-70), the sorghum is substituted for the corn completely. Setting a control ration (CTR) without added enzyme preparation in SSBM ration; 250ppm of a commercial Protease Ration (PRO) and 150ppm of pancreatin (HT308) were added. The feed is prepared according to NRC (2012), Chinese pig feeding standard (2004), Chinese feed ingredient and nutrition value table (2015), feed raw material database of Ministry of Agriculture Feed Industry Center (MAFIC) and the like, and the daily ration formula and the nutrition level are shown in Table 7.

Table 7 test diet composition and nutritional levels (%, feeding base)

Note:¹providing per kg piglet premix: vitamin a, 12,000 IU; vitamin D₃2,500 IU; vitamin E, 30 IU; vitamin K₃3 mg; vitamin B₁₂0.012 mg; riboflavin, 4 mg; nicotinic acid, 40 mg; pantothenic acid, 15 mg; choline chloride, 400 mg; folic acid, 0.7 mg; vitamin B₁1.5 mg; vitamin B₆3mg biotin, 0.1 mg; 80mg of zinc; manganese, 30 mg; iron, 90 mg; copper, 10 mg; iodine, 0.35 mg; selenium, 0.3 mg.

²The addition of the exogenous indicator of chromium oxide began 2 weeks before the end of each phase and the proportion of corn decreased accordingly.

³SID: standard ileal digestibility; lys, lysine; met, methionine; cys, cysteine; thr, threonine; trp, tryptophan.

2.3 detection index and determination method

2.3.1 growth Performance

At the 0 th, 35 th and 70 th days of the experiment, the individual weight of the pigs and the weight of the feed were weighed, and the Average Daily Gain (ADG), Average Daily Feed Intake (ADFI) and feed conversion ratio (feed efficiency) were calculated.

2.3.2 apparent digestibility of nutrient substances in Total intestinal tract

Approximately 150g of fresh manure sample was collected from each cycle daily for test periods 31-33d and 66-68d, and stored at-20 ℃. And finally, mixing the three-day feces samples, drying for 72 hours in a 65-DEG C drying oven, crushing the dried feces samples, sieving the crushed feces samples with 40 meshes, and bagging the crushed feces samples to be tested.

The determination of Dry Matter (Dry Matter, DM), Crude Protein (CP), Crude Ash (Ash), Neutral Detergent Fiber (NDF) and Acid Detergent Fiber (ADF) in the material sample and the feces sample is carried out by methods recommended by national standards GB/T6435-. Total Energy (Energy) was measured using an oxygen-nitrogen calorimeter (model 1281, Parr corporation, USA). The chromium content in the sample and feces was measured using an atomic absorption spectrometer (Hitachi Z-5000, Japan) (Yang Sheng, 1993). The apparent digestibility of each nutrient was calculated according to the indicator method.

The apparent digestibility of the nutrient total nutrient digestive system (ATTD) was determined by an indicator method. The formula is as follows: ATTD (%) 1- (DC × FN)/(FC × DN) × 100%, where DC is the concentration (%) of dichromium trioxide in feed, FN is the concentration (%) of nutrients in feces, FC is the concentration (%) of dichromium trioxide in feces, and DN is the concentration (%) of nutrients in feed. 2.3.3 determination of blood metabolites

At 34 th and 69d of the experiment, each pig was collected via the anterior vena cava using a vacuum blood collection tube (Greiner Bio-One GmbH, Austria). The anterior vena cava blood was then centrifuged at 3,000rpm for 10min to obtain serum, which was stored at-80 ℃ for serum index determination.

Plasma urea nitrogen (BUN), Total Cholesterol (TC), Total Triglyceride (TG), Total Protein (TP), and Blood Glucose (Glucose) were measured using a kit (tokyo chemie ltd., tokyo, china).

2.3.4 determination of colonies in feces

The contents of total bacteria, Escherichia coli and Lactobacillus in feces were quantified by RT-QPCR (dye method). The primer sequences are as follows:

2.4 statistical methods

All data take repetition (circle) as a statistical unit, a GLM model in SAS 9.2 statistical software is adopted for variance analysis, multiple comparisons are carried out by an SNK method, and the statistical result is obvious when P is less than 0.05.

3 results and analysis

3.1 growth Performance

No significant difference in the growth performance (ADG and ADFI) of growing pigs fed corn soybean meal type diets versus sorghum soybean meal type diets was observed at both early and late stages of growth (table 8); pancreatin and common protease in the market are added into the sorghum bean pulp type daily ration, so that the feed intake and daily gain of the growing pigs are not influenced; however, pancreatin and market protease can obviously improve the feed utilization efficiency of sorghum bean pulp type daily ration at the early stage (P is less than 0.05), and tend to improve the feed utilization rate at the later stage and the whole stage; the addition of the protease can improve the utilization rate of the sorghum soybean meal type daily ration, and the effect is equivalent to that of the corn soybean meal type daily ration.

TABLE 8 influence of pancreatin and protease addition to sorghum soybean meal type diets on growth performance of growing pigs

Note: CSBM, corn and soybean meal type ration; SSBM, sorghum bean meal type ration; CTR, no added enzyme preparation; PRO, commercial protease preparation; HT300, aureobasin protease

3.2 digestibility

In the early stage, dry matters, organic matters and total apparent digestibility of the corn bean pulp type daily ration tend to be higher than those of the sorghum bean pulp type daily ration; and pancreatin and protease commonly used in the market are added, so that the digestion rate of sorghum bean pulp type daily ration tends to be increased. The apparent digestibility of the crude protein of the corn bean pulp type daily ration is higher than that of the sorghum bean pulp type daily ration (P <0.05), and the digestibility of the crude protein of the sorghum bean pulp type daily ration is also improved by adding HT308 pancreatin and common protease in the market (P < 0.05).

In the later period, the apparent digestibility of dry matters, organic matters, total energy and crude protein of the corn bean pulp type daily ration is higher than that of the sorghum bean pulp type daily ration (P is less than 0.05), and the digestibility of nutrient substances of the sorghum bean pulp type daily ration (P is less than 0.05) is also increased by adding pancreatin and common protease in the market.

Influence of pancreatin and protease added into sorghum bean pulp type daily ration on nutrient digestibility

Note: CSBM, corn and soybean meal type ration; SSBM, sorghum bean meal type ration; CTR, no added enzyme preparation; PRO, commercial protease preparation; HT308, pancreatin.

3.3 serum metabolites

No matter in the early stage and the later stage of growth, the blood metabolic indexes of the growing pigs fed with the corn bean pulp type daily ration and the sorghum bean pulp type daily ration are not obviously different (Table 9); the addition of pancreatin and common protease in the market in sorghum soybean meal type daily ration does not affect blood metabolites of growing pigs.

TABLE 9 influence of pancreatin and protease on blood index of growing pig added to sorghum soybean meal type diet

Note: CSBM, corn and soybean meal type ration; SSBM, sorghum bean meal type ration; CTR, no added enzyme preparation; PRO, commercial protease preparation; HT308, pancreatin

3.4 fecal microorganisms

No matter in the early stage and the later stage of growth, the total bacteria, escherichia coli and lactobacillus in the pig manure for growth fed with the corn bean pulp type daily ration and the sorghum bean pulp type daily ration have no significant difference (table 10); the addition of HT308 pancreatin and the common protease in the market in the sorghum soybean meal type daily ration does not affect the flora content in the growing pig manure.

TABLE 10 Effect of pancreatin and protease addition to sorghum Bean meal type diets on growing fecal microorganisms (log CFU/g of wet digesta)

By combining the above embodiment, the preparation process for extracting the feed-grade pancreatin from the pancreatic residues can improve the feed utilization rate and increase the apparent digestibility of nutrient substances by adding the pancreatin into the sorghum soybean meal type daily ration (150ppm), without affecting the content of blood metabolites and fecal bacteria.

While the invention has been described above with reference to an embodiment, various modifications may be made and equivalents may be substituted for elements thereof without departing from the scope of the invention. In particular, the various features of the disclosed embodiments of the invention may be used in any combination, provided that no structural conflict exists, and the combinations are not exhaustively described in this specification merely for the sake of brevity and resource conservation. Therefore, it is intended that the invention not be limited to the particular embodiments disclosed, but that the invention will include all embodiments falling within the scope of the appended claims.

Claims

1. A preparation process for extracting feed-grade pancreatin from pancreatin residues is characterized in that: the preparation process for extracting the feed-grade pancreatin from the pancreatin residue comprises the following steps:

the method comprises the following steps: raw material treatment: the raw material is frozen pancreas residue obtained after extracting angiotensin from crude enzyme powder, the raw material is crushed by a flat plate crusher or a flaker, dissolved by distilled water and stirred by a stirrer for 15-30 minutes, or dissolved by pulping by a pulping machine;

2. The process of claim 1 for the extraction of feed-grade pancreatin from pancreatin pomace, wherein: the carrier for the feed is any one of corn starch, silicon dioxide, gelatin and rice hull powder.

3. The process of claim 1 for the extraction of feed-grade pancreatin from pancreatin pomace, wherein: the drying device is air-blast drying, vacuum drying or fluidized bed drying.

4. The process of claim 1 for the extraction of feed-grade pancreatin from pancreatin pomace, wherein: the dissolving temperature is controlled at 10-20 ℃, and the ratio of distilled water to raw materials is controlled at 3-1: 1.