CN110594126A - Plant non-isolated state vacuumizing device and using method thereof - Google Patents
Plant non-isolated state vacuumizing device and using method thereof Download PDFInfo
- Publication number
- CN110594126A CN110594126A CN201910874255.3A CN201910874255A CN110594126A CN 110594126 A CN110594126 A CN 110594126A CN 201910874255 A CN201910874255 A CN 201910874255A CN 110594126 A CN110594126 A CN 110594126A
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- China
- Prior art keywords
- plant
- barrel body
- bayonet
- upper cover
- isolated state
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B37/00—Pumps having pertinent characteristics not provided for in, or of interest apart from, groups F04B25/00 - F04B35/00
- F04B37/10—Pumps having pertinent characteristics not provided for in, or of interest apart from, groups F04B25/00 - F04B35/00 for special use
- F04B37/14—Pumps having pertinent characteristics not provided for in, or of interest apart from, groups F04B25/00 - F04B35/00 for special use to obtain high vacuum
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B39/00—Component parts, details, or accessories, of pumps or pumping systems specially adapted for elastic fluids, not otherwise provided for in, or of interest apart from, groups F04B25/00 - F04B37/00
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B39/00—Component parts, details, or accessories, of pumps or pumping systems specially adapted for elastic fluids, not otherwise provided for in, or of interest apart from, groups F04B25/00 - F04B37/00
- F04B39/10—Adaptations or arrangements of distribution members
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- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- General Engineering & Computer Science (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
The invention discloses a plant vacuum-pumping device in a non-isolated state, which relates to the technical field of transgenosis and comprises a barrel body, wherein the upper end of the barrel body is connected with an upper cover matched with the barrel body through a sealing mechanism, an upper bayonet is arranged on the upper edge of the barrel body, and a lower bayonet vertically corresponding to the upper bayonet is arranged on the lower edge of the upper cover. The method comprises the following steps: placing the tissue to be transgenic of the non-isolated plant in the barrel body; step two: an upper cover and the barrel body are covered to form a closed space, and an upper bayonet and a lower bayonet are used for the tissues to be transgenic to pass through; the method overcomes the defect that the woody plant can not be vacuumized when being infected by agrobacterium, the branches or flower stems of the woody plant are placed in an open vacuum container, the gaps between the upper bayonet and the lower bayonet and the plant are sealed by the semi-fluid soft rubber, and the vacuum pump is used for vacuumizing, so that the vacuumizing of the plant tissue in a non-isolated state is realized, and the agrobacterium-mediated transgenic efficiency can be obviously improved.
Description
Technical Field
The invention relates to the technical field of transgenosis, in particular to a plant vacuum-pumping device in a non-isolated state and a using method thereof.
Background
Plant transgenic technology is already applied to scientific research and breeding on a large scale, the most widely applied technology is agrobacterium-mediated transgenic technology at present, most plant tissues are difficult to permeate agrobacterium and need to be vacuumized and assisted in the infection process, herbaceous plants are small in size and tender in tissue and can be potted, the woody plants cannot be vacuumized in a non-isolated state due to the fact that the woody plants are tall and large in size, for example, the plants such as pears, loquats and blueberries cannot be vacuumized in a vacuum box of a laboratory due to large size, the plants need to be vacuumized in the non-isolated state, and no corresponding device exists at present.
The application publication No. CN202460248U discloses a vacuum filtration device, which comprises a container bottle, a funnel arranged on the container bottle, a vacuum filtration tube with one end arranged on a filtration nozzle of the container bottle, and a vacuum compression pump, wherein a filter layer is arranged in the funnel, a communicating tube device is arranged at the other end of the vacuum filtration tube, the communicating tube device is communicated with the vacuum compression pump through a vacuum filtration steel tube, a pressure control device is arranged between the communicating tube device and the vacuum compression pump, and a cooling fan is arranged on one side of the vacuum compression pump; although the device can carry out the vacuum-pumping operation, the vacuum-pumping operation of the plant in the non-isolated state cannot be realized, so that the non-isolated state vacuum-pumping device for the plant and the using method thereof are provided to solve the technical problems.
Disclosure of Invention
The invention aims to provide metal detection equipment for packaging bamboo chopsticks, which aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
the utility model provides a non-isolated state evacuating device of plant, includes the staving, the staving upper end be connected with through sealing mechanism with staving complex upper cover, the bayonet socket has been seted up on the staving upper edge, the lower bayonet socket that corresponds with last bayonet socket is vertical is seted up to the upper cover lower edge.
As a modified scheme of the invention: the upper bayonet and the lower bayonet are both in a semicircular structure.
As a modified scheme of the invention: the sealing mechanism comprises an annular spoke plate I which is sleeved and fixed on the lower edge of the upper cover and an annular spoke plate II which is sleeved and fixed on the upper edge of the barrel body and is arranged opposite to the annular spoke plate I.
As a modified scheme of the invention: the sealing mechanism further comprises a sealing ring which is attached to the barrel body in a sleeved mode, and the sealing ring is located between the annular spoke plate II and the annular spoke plate I.
As a modified scheme of the invention: an air inlet pipe is installed on the barrel body, and a valve II is installed on the air inlet pipe.
As a modified scheme of the invention: an air release pipe is installed on the upper cover, a valve I is installed at the top of the air release pipe, and a pressure gauge is installed at the top of the upper cover.
The use method of the vacuum-pumping device for the non-isolated state of the plant comprises the following steps:
the method comprises the following steps: placing a tissue part to be transgenic of a non-isolated plant inside a barrel body; adding an agrobacterium solution into the barrel body to be infected;
step two: covering the upper cover and the barrel body to form a closed space, wherein the upper bayonet and the lower bayonet are used for the tissues to be transgenic to pass through, and the tissues to be transgenic are immersed in the agrobacterium solution in the barrel body;
step three: filling and plugging the upper bayonet and the lower bayonet by using semi-flowable soft rubber;
step four: and closing the valve I, opening the valve II, and connecting the air inlet pipe with an external vacuum pump to vacuumize the interior of the barrel body.
Compared with the prior art, the invention has the beneficial effects that:
the invention overcomes the defect that the woody plant can not be vacuumized when being infected by agrobacterium, and provides a plant vacuum-pumping device and a plant vacuum-pumping method in a non-isolated state.
Drawings
FIG. 1 is a schematic structural diagram of a vacuum-pumping device for plants in a non-isolated state according to the present invention.
In the figure: 1-pressure gauge, 2-air release pipe, 3-upper cover, 4-upper bayonet, 5-lower bayonet, 6-sealing ring, 7-air inlet pipe, 8-barrel, 9-valve I, 10-annular spoke plate I, 11-annular spoke plate II and 12-valve II.
Detailed Description
The technical scheme of the patent is further described in detail by combining the following specific embodiments:
example 1
Referring to fig. 1, a plant vacuum pumping device in a non-in-vitro state comprises a barrel body 8, wherein the upper end of the barrel body 8 is connected with an upper cover 3 matched with the barrel body 8 through a sealing mechanism, an upper bayonet 4 is arranged on the upper edge of the barrel body 8, and a lower bayonet 5 vertically corresponding to the upper bayonet 4 is arranged on the lower edge of the upper cover 3.
Install intake pipe 7 on the staving 8, install valve II12 on the intake pipe 7, intake pipe 7 is used for carrying out the inside evacuation of device with outside vacuum pump connection, installs gas release pipe 2 on the upper cover 3, and valve I9 is installed at gas release pipe 2 top, and manometer 1 is installed at the 3 tops of upper cover. The pressure gauge 1 is used for observing the air pressure value inside the device in time so as to achieve a vacuum environment.
Specifically, when carrying out the non-isolated state evacuation operation of plant, the upper cover 3 and the 8 cooperation connections of staving that set up realize wrapping up the transgenic tissue of treating of non-isolated plant, seal through sealing mechanism.
The specific use method of the plant non-isolated state vacuumizing device comprises the following steps:
the method comprises the following steps: placing the tissue part to be transgenic of the non-isolated plant in the barrel body 8; an agrobacterium solution is added into the barrel body 8 to be infected;
step two: the upper cover 3 and the barrel body 8 are covered to form a closed space, the upper bayonet 4 and the lower bayonet 5 are used for the tissues to be transgenic to pass through, and the tissues to be transgenic are soaked in the agrobacterium solution in the barrel body 8; the upper bayonet 4 and the lower bayonet 5 can be used for clamping branches or stems of woody plants up and down to fix the branches or stems, the diameters of the upper bayonet 4 and the lower bayonet 5 are selected to be between 2 millimeters and 5 centimeters, and the optimal diameter is 1 centimeter, so that gene tissues can be conveniently placed.
Step three: the upper bayonet 4 and the lower bayonet 5 are filled and plugged by using semi-flowable soft rubber, so that gaps between the upper bayonet 4 and the lower bayonet 5 and a gene tissue contact gap are filled and plugged, and the sealing property inside the device is ensured.
Step four: and (3) closing the valve I9, opening the valve II12, and connecting the air inlet pipe 7 with an external vacuum pump to vacuumize the interior of the barrel body 8.
Example 2
On embodiment 1's basis, in addition, go up bayonet socket 4 and lower bayonet socket 5 and be half round structure, adopt half round structure to make go up bayonet socket 4 and lower bayonet socket 5 can realize fine laminating effect with the branch or the scape of woody plant, can be better hold and joint woody plant, convenient operation.
Further, the sealing mechanism in the device comprises an annular spoke plate I10 fixed on the lower edge of the upper cover 3 in a sleeved mode and an annular spoke plate II11 fixed on the upper edge of the barrel body 8 and opposite to the annular spoke plate I10 in a sleeved mode, and the barrel body 8 is provided with a sealing ring 6 located on the annular spoke plate II11 in a sleeved mode in a fitted mode.
When upper cover 3 and staving 8 cooperation are connected, annular radials I10 and annular radials II11 are close to relatively to be located sealing washer 6 between the two and compress tightly, the edge can closely laminate with annular radials I10 and annular radials II11 respectively about sealing washer 6, has realized the good sealed effect of this device, and evacuation efficiency obtains obviously promoting.
In conclusion, the invention overcomes the defect that the woody plant can not be vacuumized when being infected by agrobacterium, and provides the plant vacuum-pumping device and method in the non-in-vitro state, branches or flower stems of the woody plant are placed in an open vacuum container, gaps between the upper bayonet 4 and the lower bayonet 5 and the plant are sealed by using semi-fluid soft rubber, and then the vacuum-pumping is carried out through a vacuum pump, so that the vacuum-pumping of plant tissues in the non-in-vitro state is realized, and the agrobacterium-mediated transgenic efficiency can be obviously improved.
It should be noted that, although the present specification describes embodiments, each embodiment does not include only a single technical solution, and such description of the specification is only for clarity, and those skilled in the art should take the specification as a whole, and the technical solutions in the embodiments may be appropriately combined to form other embodiments understood by those skilled in the art, and the above-mentioned embodiments only express the preferred embodiments of the technical solutions, and the description thereof is more specific and detailed, but should not be construed as limiting the scope of the claims of the technical solutions. It should be noted that, for those skilled in the art, various modifications, improvements and substitutions can be made without departing from the spirit of the invention, and all of them belong to the protection scope of the technical solution. The protection scope of this technical solution patent should be subject to the appended claims.
Claims (7)
1. The utility model provides a non-separation state evacuating device of plant, includes staving (8), its characterized in that, staving (8) upper end be connected with through sealing mechanism with staving (8) complex upper cover (3), upper bayonet socket (4) have been seted up on staving (8), upper cover (3) lower edge seted up with the vertical lower bayonet socket (5) that correspond of upper bayonet socket (4).
2. The plant vacuum pumping device in the non-isolated state according to claim 1, wherein the upper bayonet (4) and the lower bayonet (5) are both semicircular structures.
3. The plant vacuum pumping device in the non-isolated state according to claim 1, wherein the sealing mechanism comprises an annular web I (10) sleeved and fixed on the lower edge of the upper cover (3) and an annular web II (11) sleeved and fixed on the upper edge of the barrel body (8) and arranged opposite to the annular web I (10).
4. The plant vacuum pumping device in the non-separated state according to claim 1, wherein the sealing mechanism further comprises a sealing ring (6) fitted on the barrel body (8), and the sealing ring (6) is located between the annular web II (11) and the annular web I (10).
5. The plant vacuum extractor in the non-isolated state as claimed in claim 1, wherein the barrel (8) is provided with an air inlet pipe (7), and the air inlet pipe (7) is provided with a valve II (12).
6. The plant vacuum extractor in the non-isolated state according to claim 1, wherein the upper cover (3) is provided with an air discharging pipe (2), the top of the air discharging pipe (2) is provided with a valve I (9), and the top of the upper cover (3) is provided with a pressure gauge (1).
7. The use method of the plant non-isolated state vacuum pumping device according to any one of claims 1-6, characterized by comprising the following steps:
the method comprises the following steps: placing a tissue part to be transgenic of a non-isolated plant into a barrel body (8), and adding an agrobacterium solution into the barrel body (8) to be infected;
step two: the upper cover (3) and the barrel body (8) are covered to form a closed space, the upper bayonet (4) and the lower bayonet (5) are used for the tissues to be transgenic to pass through, and the tissues to be transgenic are soaked in the agrobacterium solution in the barrel body (8);
step three: filling and plugging the upper bayonet (4) and the lower bayonet (5) by using semi-flowable soft rubber;
step four: and (3) closing the valve I (9), opening the valve II (12), and connecting the air inlet pipe (7) with an external vacuum pump to vacuumize the interior of the barrel body (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910874255.3A CN110594126A (en) | 2019-09-17 | 2019-09-17 | Plant non-isolated state vacuumizing device and using method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910874255.3A CN110594126A (en) | 2019-09-17 | 2019-09-17 | Plant non-isolated state vacuumizing device and using method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110594126A true CN110594126A (en) | 2019-12-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910874255.3A Pending CN110594126A (en) | 2019-09-17 | 2019-09-17 | Plant non-isolated state vacuumizing device and using method thereof |
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| Country | Link |
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| CN (1) | CN110594126A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101216025A (en) * | 2008-01-10 | 2008-07-09 | 河南农业大学 | Simple vacuum pumping device, vacuum sampling bottle and vacuum pumping method |
| CN101457235A (en) * | 2008-08-05 | 2009-06-17 | 山东省林业科学研究院 | Method for converting lucerne by vacuum penetrating auxiliary agrobacterium-mediated |
| CN104071463A (en) * | 2013-03-30 | 2014-10-01 | 赵百华 | Bi-functional vacuum tank |
| CN104195170A (en) * | 2014-09-16 | 2014-12-10 | 云南省农业科学院生物技术与种质资源研究所 | Rice vacuum penetration genetic transformation method |
| CN204357659U (en) * | 2014-12-31 | 2015-05-27 | 长兴添辰模具有限公司 | A kind of vacuum pumping device |
| CN204443315U (en) * | 2015-01-06 | 2015-07-08 | 中国农业科学院北京畜牧兽医研究所 | A kind of alfalfa Non in vitro Rapid Rooting sticking device |
-
2019
- 2019-09-17 CN CN201910874255.3A patent/CN110594126A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101216025A (en) * | 2008-01-10 | 2008-07-09 | 河南农业大学 | Simple vacuum pumping device, vacuum sampling bottle and vacuum pumping method |
| CN101457235A (en) * | 2008-08-05 | 2009-06-17 | 山东省林业科学研究院 | Method for converting lucerne by vacuum penetrating auxiliary agrobacterium-mediated |
| CN104071463A (en) * | 2013-03-30 | 2014-10-01 | 赵百华 | Bi-functional vacuum tank |
| CN104195170A (en) * | 2014-09-16 | 2014-12-10 | 云南省农业科学院生物技术与种质资源研究所 | Rice vacuum penetration genetic transformation method |
| CN204357659U (en) * | 2014-12-31 | 2015-05-27 | 长兴添辰模具有限公司 | A kind of vacuum pumping device |
| CN204443315U (en) * | 2015-01-06 | 2015-07-08 | 中国农业科学院北京畜牧兽医研究所 | A kind of alfalfa Non in vitro Rapid Rooting sticking device |
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Application publication date: 20191220 |
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| RJ01 | Rejection of invention patent application after publication |