CN110567860A - Novel particle counter and particle counting method - Google Patents
Novel particle counter and particle counting method Download PDFInfo
- Publication number
- CN110567860A CN110567860A CN201910799116.9A CN201910799116A CN110567860A CN 110567860 A CN110567860 A CN 110567860A CN 201910799116 A CN201910799116 A CN 201910799116A CN 110567860 A CN110567860 A CN 110567860A
- Authority
- CN
- China
- Prior art keywords
- particles
- impedance
- signal
- impedance signal
- counting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000002245 particle Substances 0.000 title claims abstract description 133
- 238000000034 method Methods 0.000 title claims abstract description 28
- 230000005540 biological transmission Effects 0.000 claims abstract description 94
- 230000008859 change Effects 0.000 claims abstract description 34
- 238000001514 detection method Methods 0.000 claims abstract description 29
- 230000001939 inductive effect Effects 0.000 claims abstract description 8
- 238000012546 transfer Methods 0.000 claims description 9
- 238000004804 winding Methods 0.000 claims description 8
- 239000002609 medium Substances 0.000 description 37
- 238000004519 manufacturing process Methods 0.000 description 8
- 239000011859 microparticle Substances 0.000 description 8
- 238000013461 design Methods 0.000 description 7
- 230000006872 improvement Effects 0.000 description 7
- 238000004820 blood count Methods 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical group [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 238000000149 argon plasma sintering Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000003792 electrolyte Substances 0.000 description 3
- 230000004907 flux Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000006163 transport media Substances 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 238000013480 data collection Methods 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
Landscapes
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Materials By The Use Of Magnetic Means (AREA)
Abstract
The invention discloses a novel particle counter, which comprises a transmission tube, an inductance coil, a power supply circuit, an impedance detection circuit and a counting controller, wherein the transmission tube is connected with the inductance coil; the inductance coil is wound on the transmission tube to form a hollow inductance element; the power supply circuit is used for supplying power to the inductive element; the impedance detection circuit is used for detecting the impedance signal of the inductive element in real time, wherein when the transmission medium is introduced into the transmission pipe, the impedance signal acquired by the impedance detection circuit in real time is a first impedance signal, and when the particles and the transmission medium are mixed so that the particles are introduced into the transmission pipe along with the transmission medium, the impedance signal acquired by the impedance detection circuit in real time is a second impedance signal; the counting controller is used for comparing the first impedance signal with the second impedance signal to generate a pulse change signal, and calculating the number of particles and identifying the type of the particles according to the pulse change signal. The invention also discloses a particle counting method. The invention can realize the automatic counting and identification of particles, and has accurate counting and simple and convenient operation.
Description
Technical Field
The invention relates to the technical field of inductance counting, in particular to a novel particle counter and a particle counting method based on the novel particle counter.
Background
The requirements of modern high-precision machinery on a dust-free environment, the detection of PM2.5 in air and the research of biomedicine on cells all make the research on the quantity and the state of particles in the microscopic field more urgent, and the counting technology of microscopic particles is very important. The conventional microscopic particle counting methods include a blood cell counting method, a light scattering particle counter, a coulter principle, and the like.
First, a blood cell counting method. The hemacytometer was performed on a hemacytometer plate constructed as shown in fig. 1. The blood counting plate is used for microscopic counting of red blood cells and white blood cells in human bodies, is also commonly used for counting of some microorganisms such as bacteria, fungi, yeasts and the like, and is a common biological tool.
The method comprises the following operation steps:
S1, preparing a suspension of bacteria to be detected;
S2, taking a clean blood counting chamber, and covering a cover glass on the counting area;
S3, shaking the bacterial liquid evenly, and sucking a little by a dropper to drip from the edge of the side edge of the counting plate platform;
S4, resting to allow the cells to settle on the counting plate until they no longer drift with the liquid, and then placing under a microscope for counting.
However, the counting process of the blood cell counting method is completed by manual operation, the counting operation process is complicated, and repeated operation is needed for many times for improving the counting precision on one hand, and professional personnel are also needed on the other hand, so that the accuracy of final counting can be ensured. In addition, this method takes a long time.
And secondly, a light scattering particle counter. The working principle of the light scattering particle counter is as follows: the light from the light source is focused in the measuring cavity of the sensor, a vacuum device is arranged at the outlet of the sensor, air is pumped out through the sensor, and when each particle in the air rapidly passes through the measuring cavity, the incident light is scattered once to form an optical pulse signal; the scattered light is focused on a photoelectric detector by a rear condenser lens, and then the light is converted into a voltage signal; the microprocessor can connect the counter to the control data collection system through the interface, and finally the counter is displayed through the counting system, so that the counting work of a large number of electric pulses is completed.
However, the counting result of the light scattering particle counter is relatively low in accuracy, the detection result of the method is more favorable for detection of an order of magnitude, and the method requires the design and manufacture of a separate external device, so that the use cost is relatively high generally.
and thirdly, a Coulter principle. As shown in fig. 2, when the particles suspended in the electrolyte pass through the small-hole tube along with the electrolyte, the same volume of electrolyte is replaced, and the resistance between the two electrodes inside and outside the small-hole tube is instantaneously changed in the constant-current designed circuit, so that potential pulses are generated. The magnitude and number of pulse signals is proportional to the size and number of particles and can be used for blood cell counting.
Because the method belongs to the measurement of individual particles and the three-dimensional measurement, the method not only can accurately measure the particle size distribution of the material, but also can measure the absolute number and concentration of particles. The measured particle size is closer to the real particle size and is not influenced by the color and the concentration of the material like the laser diffraction scattering principle.
However, the coulter principle requires a separate design of the electrodes, and the overall design accuracy is required to be high, thereby realizing detection. During the process of cell detection, the micro flow channel needs to be specially designed and manufactured, which increases the manufacturing difficulty and cost and needs to deposit electrodes by a deposition method.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a novel particle counter and a particle counting method based on the novel particle counter, which can realize automatic counting and identification of particles, and have accurate counting and simple and convenient operation.
In order to solve the above technical problems, the present invention provides a novel particle counter, including: the device comprises a transmission pipe, an inductance coil, a power supply circuit, an impedance detection circuit and a counting controller; the inductance coil is wound on the transmission tube to form a hollow inductance element; the power supply circuit is used for supplying power to the inductance element; the impedance detection circuit is used for acquiring an impedance signal of the inductance element in real time, wherein the impedance signal acquired by the impedance detection circuit in real time is a first impedance signal when a transmission medium is introduced into the transmission pipe, and the impedance signal acquired by the impedance detection circuit in real time is a second impedance signal when particles and the transmission medium are mixed so that the particles are introduced into the transmission pipe along with the transmission medium; the counting controller is used for comparing the first impedance signal with the second impedance signal to generate a pulse change signal, calculating the number of particles according to the pulse number of the pulse change signal, and identifying the type of the particles according to the pulse amplitude of the pulse change signal.
As an improvement of the above scheme, a signal amplifier for amplifying the impedance signal is arranged in the impedance detection circuit.
as an improvement of the scheme, the inductance coil is wound on the transmission tube by adopting a honeycomb winding method.
As an improvement of the scheme, the transmission pipe is a capillary pipe.
as an improvement of the above, the flow cross-sectional area of the transfer tube is at least 1.5 times the maximum cross-sectional area of the microparticles.
In an improvement of the above aspect, the wire diameter of the inductor is 0.1 to 0.5 times the particle diameter of the fine particles.
As an improvement of the scheme, the number of turns of the inductance coil is 3-9 turns.
As a modification of the above, the transport medium is air or a constant solution.
As an improvement of the scheme, plug-in connectors are arranged at two ends of the transmission pipe.
Correspondingly, the invention also provides a particle counting method based on the novel particle counter, which comprises the following steps: introducing a transmission medium into a transmission pipe, and acquiring a first impedance signal of an inductive element in real time; mixing the particles with a transmission medium to enable the particles to be introduced into the transmission pipe along with the transmission medium, and acquiring a second impedance signal of the inductance element in real time; comparing the first impedance signal with a second impedance signal to generate a pulse change signal; calculating the number of particles according to the number of pulses of the pulse change signal; and identifying the type of the particles according to the pulse amplitude of the pulse change signal.
The implementation of the invention has the following beneficial effects:
The invention firstly proposes that the inductive coil is used for counting and identifying the microparticles, complex external equipment is not needed, and the automatic counting and identification of the microparticles can be realized without redesigning a micro-channel in the original micro-fluidic chip. Specifically, the pure transmission medium and the transmission medium mixed with particles are sequentially input into the transmission pipe to respectively acquire the impedance signals of the inductance element in the two states, and the number and the type of the particles are identified according to the pulse change generated by the impedance signals twice, so that the flexibility is strong, and the accuracy is high.
furthermore, the invention can use honeycomb winding method to wind the inductance coil, so that the inductance of the inductance element can be ensured in a smaller volume, the counting is accurate, and the operation is simple and convenient;
In addition, the invention can design the inductance coil and the transmission pipe as independent plug-in units, thereby simplifying the manufacture of the inductance coil and the transmission pipe, ensuring the counting precision and reducing the counting cost of particles.
Drawings
FIG. 1 is a schematic diagram of a conventional blood cell counting plate, wherein A is a front view, B is a side view, C is an enlarged grid, and D is an enlarged counting chamber;
FIG. 2 is a prior art Kort diagram;
FIG. 3 is a schematic diagram of the structure of the novel particle counter of the present invention;
FIG. 4 is a schematic view of the present invention with a transfer medium being fed into the transfer tube;
FIG. 5 is a schematic view of the present invention when a transport medium mixed with particles is fed into a transport pipe;
FIG. 6 is a schematic diagram of the structure of the honeycomb winding method in the novel particle counter of the present invention;
Fig. 7 is a flow chart of the particle counting method based on the novel particle counter.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the accompanying drawings.
Referring to fig. 3, fig. 3 shows a specific structure of the novel particle counter of the present invention, which includes a transmission tube 1, an inductance coil 2, a power supply circuit 3, an impedance detection circuit 4 and a counting controller 5. Specifically, the method comprises the following steps:
the inductance coil 2 is wound on the transmission tube 1 to form a hollow inductance element L.
the power supply circuit 3 is configured to supply power to the inductance element L. Preferably, the power supply circuit is a variable frequency constant voltage circuit, but not limited thereto.
The impedance detection circuit 4 is configured to acquire an impedance signal of the inductance element L in real time, where the impedance signal acquired by the impedance detection circuit 4 in real time is a first impedance signal when the transmission medium 6 is introduced into the transmission tube 1, and the impedance signal acquired by the impedance detection circuit 4 in real time is a second impedance signal when the particles 7 are mixed with the transmission medium 6 so that the particles 7 are introduced into the transmission tube 1 together with the transmission medium 6.
The counting controller 5 is configured to compare the first impedance signal with the second impedance signal to generate a pulse variation signal, calculate the number of particles according to the number of pulses of the pulse variation signal, and identify the type of the particles according to the pulse amplitude of the pulse variation signal.
As can be seen from fig. 4 and 5, a small hollow inductor L can be formed by winding the inductor coil 2 around the transmission pipe 1. When the transmission medium 6 is introduced into the transmission tube 1 (see fig. 4), the transmission medium 6 acts as an "iron core" inside the hollow inductance element L, and the dielectric constant is constant because the transmission medium 6 is constant, so that the inductance value of the whole inductance element L is constant, and a stable impedance signal is formed; however, when the particles 7 are mixed with the transmission medium 6 and the particles 7 suspended in the transmission medium 6 are introduced into the transmission pipe 1 together with the transmission medium 6 (see fig. 5), the particles 7 replace the transmission medium 6 with the same volume, so that the dielectric constant of the transmission medium 6 is changed, that is, the "iron core" inside the inductance element L is changed instantaneously, and thus the inductance value of the inductance element L is changed, and the impedance signal of the inductance element L is changed.
From the formula XLAs can be seen from 2 pi fL, the inductance is proportional to the inductance, and the inductance is an inherent characteristic of the inductor 2, and is related only to the number of turns, size, and shape of the inductor 2, and the transmission medium 6. Accordingly, when the particles 7 enter the inductance coil 2, the transmission medium of the inductance coil 2 is changed, so that the inductance is changed, the change of the inductance coil 2 caused by the different sizes of the particles 7 is naturally different, and similarly, the change of the transmission medium 6 caused by different particles 7 is also different, so that the counting and the identification can be realized. Therefore, the size and the number of the pulse change signals formed by the difference of the two impedance signals are in direct proportion to the size and the number of the particles 7, specifically, the pulse number of the pulse change signals is the particle number, and the particle type can be identified through the pulse amplitude of the pulse change signals.
Further, a signal amplifier for amplifying the impedance signal is arranged in the impedance detection circuit 4, so that the impedance signal can be compared and calculated conveniently by the counting controller 5.
In addition, the conveying pipe 1 is preferably a capillary, which can effectively ensure that only a single particle passes through the conveying pipe 1 each time, thereby ensuring accurate collection of the number of the particles 7. Meanwhile, the transmission medium 6 may be air or a constant solution, but is not limited thereto as long as the stability of the transmission medium 6 can be ensured.
Therefore, the invention firstly proposes that the inductance coil 2 is used for counting and identifying the microparticles, complex external equipment is not needed, and the automatic counting and identification of the microparticles can be realized without redesigning a micro-channel in the original micro-fluidic chip.
As shown in fig. 6, the inductor 2 is wound around the transfer tube 1 by honeycomb winding.
It should be noted that, because the invention needs to realize particle detection, the inductance coil 2 used in the invention is necessarily required to be wound in a very small space, and a certain inductance value needs to be generated so as to ensure the strength of the impedance signal. Therefore, the invention adopts the honeycomb winding method to manufacture the inductance coil 2, so that the manufactured inductance coil 2 has small volume, small distributed capacitance and large inductance.
Further, the flow cross-sectional area of the transfer tube 1 is at least 1.5 times the maximum cross-sectional area of the particles 7, thereby ensuring smooth passage of the individual particles 7.
Meanwhile, the wire diameter of the inductance coil 2 is 0.1-0.5 times of the particle diameter of the particles 7; the number of turns of the inductance coil 2 is 3-9 turns.
it should be noted that the inductance value of the inductor coil is changed to affect the inductance of the whole circuit, and the inductance value formula is: l is N2μAe/leThe equation shows the equivalent magnetic circuit sectional area A of the square sum of the inductance L and the number N of turns of the winding seteIs proportional to the equivalent magnetic path length leInversely proportional, in order to make the coil normally work the coil turns at least more than 3 turns, and the coil should not be too long or the same has the adverse effect (in addition, in order to ensure that only one particle exists in the inductance coil when the magnetic flux changes each time, the coil turns too much should be avoided as much as possible), in order to make the particle pass and have sufficient magnetic flux change, the particle size is at least 1/3 of the coil length; similarly, in order to ensure smooth circulation of particles in the channel, the particle size should be less than 1/3 of the cross-sectional area of the channel, but in order to ensure that the magnetic flux of the coil is sufficiently influenced when the particles pass through the inside of the inductance coil, the particle size/the cross-sectional area of the coil should be set as large as possible.
In the invention, the inductance coil 2 is closely attached to the pipe wall of the transmission pipe 1, the inductance coil 2 is tightly wound, and the wire diameter can be selected according to the size of the measured micro-particles 7. For example, if particles 7 having a diameter of 100 μm are detected, an inductor 2 having a wire diameter of 10 to 50 μm may be used, the number of turns of the inductor 2 is 5, and the flow rate of the transmission medium 6 is controlled to ensure that a single particle 7 is passed through each time.
furthermore, plug-in connectors are arranged at two ends of the transmission pipe 1. Preferably, the card interface may be a slot, a flange, or the like, but is not limited thereto, as long as the inductance element L can be fixed to the cell sorting outlet.
it should be noted that, in the present invention, the inductance coil 2 is wound on the transmission tube 1 to form the hollow inductance element L, so that the inductance element L can be designed as an independent plug-in, which, on one hand, reduces the manufacturing difficulty of the inductance element L, simplifies the equipment, does not need the design and manufacture of an independent counting chip, and on the other hand, makes the detection more convenient. Specifically, by arranging the plug-in interfaces at the two ends of the transmission pipe 1, when counting the particles 7, the particles can be counted only by fixing the plug-in (the inductance element L) at a specific position (such as a cell sorting outlet) through the plug-in interfaces, so that the particles 7 enter the transmission pipe 1 of the plug-in (the inductance element L) through the plug-in interfaces, and automatic counting can be realized, and the counting is convenient and fast.
therefore, the honeycomb type inductance coil is used, so that the inductance value of the honeycomb type inductance coil is ensured in a certain smaller volume, the counting is accurate and convenient, and the operation is simple and convenient; in addition, the invention can design independent plug-ins for the inductance coil 2 and the transmission pipe 1, so that the manufacture of the invention is simple and convenient, and the counting cost of the particles is reduced while the counting precision is ensured.
Referring to fig. 7, fig. 7 shows a flow chart of an embodiment of the particle counting method based on the novel particle counter, and it should be noted that, in the present invention, the novel particle counter includes a transmission tube 1, an inductance coil 2, a power supply circuit 3, an impedance detection circuit 4 and a counting controller 5, and the inductance coil 2 is wound on the transmission tube 1 to form a hollow inductance element L (see fig. 3).
Specifically, the particle counting method based on the novel particle counter comprises the following steps:
S101, introducing a transmission medium into a transmission pipe, and acquiring a first impedance signal of the inductance element in real time (see figure 4).
And S102, mixing the particles with a transmission medium to enable the particles to be introduced into the transmission pipe along with the transmission medium, and acquiring a second impedance signal of the inductance element in real time (see figure 5).
It should be noted that there is no inevitable sequence between the steps S101 and S102, and the step S101 may be performed first and then the step S102 may be performed, or the step S102 may be performed first and then the step S101 may be performed.
S103, comparing the first impedance signal with the second impedance signal to generate a pulse change signal.
from the formula XLAs can be seen from 2 pi fL, the inductive reactance is proportional to the inductance, and the inductive reactance is an inherent characteristic of the inductor coil, and is related only to the number of turns, size, and shape of the inductor coil and the transmission medium. Correspondingly, when the particles enter the inductance coil, the transmission medium of the inductance coil is changed, so that the inductance is changed, the change of the inductance coil caused by the different sizes of the particles is naturally different, and similarly, the change of the transmission medium caused by different particles is also different, so that the counting and the identification can be realized. Therefore, the magnitude and the number of pulse change signals formed by the difference of the two impedance signals are related to the magnitude and the number of particles.
And S104, calculating the number of particles according to the pulse number of the pulse change signal.
It should be noted that, in the present invention, the pipe diameter of the transmission pipe is set according to the size of the particles, and the flow rate of the transmission medium is controlled, so that only a single particle passes through the transmission pipe every time, therefore, every time a particle passes through the transmission pipe, a pulse change is generated, and the pulse number in the obtained pulse change signal is the particle number.
and S105, identifying the type of the particles according to the pulse amplitude of the pulse change signal.
Since the change of the transmission medium caused by different types of particles is also different, the type of particles can be identified from the pulse amplitude of the pulse change signal.
it should be noted that there is no inevitable order between step S104 and step S105, and step S105 may be performed at the same time as step S104.
when the transmission device works, a transmission medium is firstly introduced into the transmission pipe, and the impedance detection circuit acquires a first impedance signal of the inductance element in real time; then, mixing the particles with a transmission medium to enable the particles to be introduced into the transmission pipe along with the transmission medium, and acquiring a second impedance signal of the inductance element by an impedance detection circuit in real time; the counting controller compares the first impedance signal with the second impedance signal to generate a pulse change signal, calculates the number of particles according to the pulse number of the pulse change signal, and identifies the type of the particles according to the pulse amplitude of the pulse change signal.
Therefore, the invention firstly proposes that the inductance coil is used for counting and identifying the microparticles, no complex external equipment is needed, and no redesign is needed for a micro-channel in the original micro-fluidic chip, so that the automatic counting and identification of the microparticles can be realized; meanwhile, the honeycomb type inductance coil is used, so that the inductance value of the honeycomb type inductance coil is ensured in a certain smaller volume, the counting is accurate, and the operation is simple and convenient; in addition, the invention can design the inductance coil and the transmission pipe as independent plug-in units, thereby simplifying the manufacture of the inductance coil and the transmission pipe, ensuring the counting precision and reducing the counting cost of particles.
While the foregoing is directed to the preferred embodiment of the present invention, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention.
Claims (10)
1. a novel particle counter is characterized by comprising a transmission tube, an inductance coil, a power supply circuit, an impedance detection circuit and a counting controller;
The inductance coil is wound on the transmission tube to form a hollow inductance element;
The power supply circuit is used for supplying power to the inductance element;
The impedance detection circuit is used for acquiring an impedance signal of the inductance element in real time, wherein the impedance signal acquired by the impedance detection circuit in real time is a first impedance signal when a transmission medium is introduced into the transmission pipe, and the impedance signal acquired by the impedance detection circuit in real time is a second impedance signal when particles and the transmission medium are mixed so that the particles are introduced into the transmission pipe along with the transmission medium;
The counting controller is used for comparing the first impedance signal with the second impedance signal to generate a pulse change signal, calculating the number of particles according to the pulse number of the pulse change signal, and identifying the type of the particles according to the pulse amplitude of the pulse change signal.
2. The novel particle counter of claim 1, wherein a signal amplifier for amplifying the impedance signal is provided in said impedance detection circuit.
3. the novel particle counter of claim 1, wherein said inductive coil is wound on said transfer tube using honeycomb winding.
4. The novel particle counter of claim 1 wherein said transfer tube is a capillary tube.
5. A novel particle counter as claimed in claim 1 wherein said transfer tube has a cross-sectional flow area of at least 1.5 times the maximum cross-sectional area of said particles.
6. The novel particle counter according to claim 1, wherein the wire diameter of the inductance coil is 0.1 to 0.5 times the particle diameter of the particles.
7. a novel particle counter as claimed in claim 1, wherein said inductor coil has 3 to 9 turns.
8. the novel particle counter of claim 1, wherein said transmission medium is air or a constant solution.
9. The novel particle counter of claim 1 wherein said transfer tube is provided with plug-in ports at both ends.
10. A particle counting method based on the novel particle counter according to any one of claims 1 to 9, comprising:
Introducing a transmission medium into a transmission pipe, and acquiring a first impedance signal of an inductive element in real time;
Mixing the particles with a transmission medium to enable the particles to be introduced into the transmission pipe along with the transmission medium, and acquiring a second impedance signal of the inductance element in real time;
Comparing the first impedance signal with a second impedance signal to generate a pulse change signal;
Calculating the number of particles according to the number of pulses of the pulse change signal;
And identifying the type of the particles according to the pulse amplitude of the pulse change signal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910799116.9A CN110567860A (en) | 2019-08-28 | 2019-08-28 | Novel particle counter and particle counting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910799116.9A CN110567860A (en) | 2019-08-28 | 2019-08-28 | Novel particle counter and particle counting method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110567860A true CN110567860A (en) | 2019-12-13 |
Family
ID=68776363
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910799116.9A Pending CN110567860A (en) | 2019-08-28 | 2019-08-28 | Novel particle counter and particle counting method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110567860A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113193737A (en) * | 2021-04-15 | 2021-07-30 | 常州易控汽车电子股份有限公司 | IGBT module |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0157496A2 (en) * | 1984-03-28 | 1985-10-09 | Northern Telecom Limited | Monitoring of magnetically permeable particles in a carrier material |
| WO2006075049A1 (en) * | 2005-01-14 | 2006-07-20 | Valtion Teknillinen Tutkimuskeskus | Device for determining metallic contaminant particles in lubricating grease |
| CN201852771U (en) * | 2010-10-29 | 2011-06-01 | 大连海事大学 | Oil grain counter |
| CN202002870U (en) * | 2010-12-01 | 2011-10-05 | 张洪朋 | Oil liquid particle distinguished counting device |
| CN102486448A (en) * | 2010-12-01 | 2012-06-06 | 张洪朋 | Oil particle distinguishing counter |
| CN202994624U (en) * | 2012-12-06 | 2013-06-12 | 江苏苏净集团有限公司 | Counting system of particle counter |
| CN206479431U (en) * | 2016-10-13 | 2017-09-08 | 赤峰锐蓝智控科技有限公司 | Oil liquid monitoring sensor and equipment |
| CN107340226A (en) * | 2017-08-03 | 2017-11-10 | 江苏大学 | Suspended particulates counting and detecting device and its application in a kind of fluid |
| CN109470606A (en) * | 2018-11-02 | 2019-03-15 | 大连海事大学 | A kind of microfluid inductance type oil liquid detection device |
| CN109813762A (en) * | 2019-03-14 | 2019-05-28 | 大连海事大学 | A kind of inductance type magnetic nano material sensor and production method |
-
2019
- 2019-08-28 CN CN201910799116.9A patent/CN110567860A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0157496A2 (en) * | 1984-03-28 | 1985-10-09 | Northern Telecom Limited | Monitoring of magnetically permeable particles in a carrier material |
| WO2006075049A1 (en) * | 2005-01-14 | 2006-07-20 | Valtion Teknillinen Tutkimuskeskus | Device for determining metallic contaminant particles in lubricating grease |
| CN201852771U (en) * | 2010-10-29 | 2011-06-01 | 大连海事大学 | Oil grain counter |
| CN202002870U (en) * | 2010-12-01 | 2011-10-05 | 张洪朋 | Oil liquid particle distinguished counting device |
| CN102486448A (en) * | 2010-12-01 | 2012-06-06 | 张洪朋 | Oil particle distinguishing counter |
| CN202994624U (en) * | 2012-12-06 | 2013-06-12 | 江苏苏净集团有限公司 | Counting system of particle counter |
| CN206479431U (en) * | 2016-10-13 | 2017-09-08 | 赤峰锐蓝智控科技有限公司 | Oil liquid monitoring sensor and equipment |
| CN107340226A (en) * | 2017-08-03 | 2017-11-10 | 江苏大学 | Suspended particulates counting and detecting device and its application in a kind of fluid |
| CN109470606A (en) * | 2018-11-02 | 2019-03-15 | 大连海事大学 | A kind of microfluid inductance type oil liquid detection device |
| CN109813762A (en) * | 2019-03-14 | 2019-05-28 | 大连海事大学 | A kind of inductance type magnetic nano material sensor and production method |
Non-Patent Citations (1)
| Title |
|---|
| 张洪明等: "基于空间微螺线管电感的微米级磨粒检测研究", 《机床与液压》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113193737A (en) * | 2021-04-15 | 2021-07-30 | 常州易控汽车电子股份有限公司 | IGBT module |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2017276244B2 (en) | Automated set-up for cell sorting | |
| Zimmermann et al. | Transcellular ion flow in Escherichia coli B and electrical sizing of bacterias | |
| US4612614A (en) | Method of analyzing particles in a fluid sample | |
| CN107202903A (en) | Sample analyser and its method of sample analysis | |
| CN105388099B (en) | Urine analyzer and method for analyzing abnormal cells in urine or body fluid | |
| EP2913657B1 (en) | Sample analyzer and sample analyzing method | |
| CN104487820A (en) | Automated and accurate drop delay for flow cytometry | |
| WO2014133160A1 (en) | Urine sample analysis device and urine sample analysis method | |
| CN106772134A (en) | A kind of apparatus and method of automatic field compensation | |
| CN110567860A (en) | Novel particle counter and particle counting method | |
| EP2843409B1 (en) | Urine sample analyzing method and sample analyzer | |
| CN113866074B (en) | Position compensation-based electrical impedance method for classifying and counting white blood cells | |
| CN207964570U (en) | A kind of adjustable grain count device of accuracy of detection | |
| CN210269556U (en) | Flow cytometer scattered light and fluorescence detection device | |
| CN208443697U (en) | A kind of nano particle diameter distribution detector | |
| CN205941574U (en) | Full -automatic unimolecule electricity nature measurement system | |
| CN104181084A (en) | Aerosol sensor | |
| Byerly et al. | Machine for rapidly counting and measuring the size of small nematodes | |
| CN108226013B (en) | Device and method for improving particle detection precision and flux by electric field focusing | |
| CN208155808U (en) | A kind of device improving particle detections precision and flux using static focusing | |
| WO2019102038A1 (en) | Flow cytometer arrangement | |
| CN108279202A (en) | A kind of adjustable grain count device and method of accuracy of detection | |
| Leif et al. | Electronic cell-volume analysis by use of the AMAC I transducer | |
| CN108254296A (en) | Wheaten starch grain Particle size analysis methods | |
| CN106483101A (en) | Urine sample analytical equipment and urine sample analysis method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191213 |
|
| RJ01 | Rejection of invention patent application after publication |