CN110554180A - Same-tray detection device for protein blotting method and immunofluorescence method - Google Patents
Same-tray detection device for protein blotting method and immunofluorescence method Download PDFInfo
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- CN110554180A CN110554180A CN201910957196.6A CN201910957196A CN110554180A CN 110554180 A CN110554180 A CN 110554180A CN 201910957196 A CN201910957196 A CN 201910957196A CN 110554180 A CN110554180 A CN 110554180A
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- 238000001514 detection method Methods 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000010166 immunofluorescence Methods 0.000 title claims abstract description 12
- 102000004169 proteins and genes Human genes 0.000 title abstract description 4
- 108090000623 proteins and genes Proteins 0.000 title abstract description 4
- 238000011534 incubation Methods 0.000 claims abstract description 34
- 239000007788 liquid Substances 0.000 claims abstract description 15
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 14
- 239000002699 waste material Substances 0.000 claims abstract description 14
- 238000002347 injection Methods 0.000 claims abstract description 11
- 239000007924 injection Substances 0.000 claims abstract description 11
- 238000001262 western blot Methods 0.000 claims abstract description 11
- 238000001035 drying Methods 0.000 claims abstract description 9
- 238000007789 sealing Methods 0.000 claims abstract description 6
- 239000012528 membrane Substances 0.000 claims description 9
- 238000003556 assay Methods 0.000 claims description 2
- 238000001917 fluorescence detection Methods 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- 238000006243 chemical reaction Methods 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 3
- 238000009739 binding Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 238000007599 discharging Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 230000002998 immunogenetic effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses a protein blotting and immunofluorescence same-plate detection device, which relates to the field of medical machinery, and adopts the following technical scheme: the device for detecting the same tray by the western blotting method and the immunofluorescence method comprises a base, a first detection device, a second detection device, an automatic sample introduction device, a sample adding treatment device and a reagent bottle area; the first detection device comprises a first incubation disc, a first waste discharge device, a first liquid injection device, a first drying device and a first scanning device, wherein the first waste discharge device, the first liquid injection device, the first drying device and the first scanning device are arranged on the side edge of the first incubation disc; the second detection device comprises a second incubation disc, a second waste discharge device, a second liquid injection device, a second sealing device and a second scanning device, wherein the second waste discharge device, the second liquid injection device, the second sealing device and the second scanning device are arranged on the side edge of the second incubation disc. The invention has the advantages of realizing simultaneous western blot detection and fluorescence detection, automatically generating contrast data, greatly improving the working efficiency and saving manpower, material resources and financial resources.
Description
Technical Field
The invention relates to the field of medical machinery, in particular to a protein blotting method and immunofluorescence method one-tray detection device.
background
The immunofluorescence technique, also called fluorescent antibody technique, is the earliest developed one in the labeled immunity technique, and is a technique established on the basis of immunology, biochemistry and microscope techniques. Western blotting (immunoblot assay) is a commonly used experimental procedure in molecular biology, biochemistry and immunogenetics. Both detection methods are widely applied, but the two detection methods are independent detection at present, the respective detection equipment is complex in operation, full-automatic operation cannot be realized, the detection speed is low, and the detection cost is high.
Disclosure of Invention
In order to solve the above mentioned problems, the present invention provides a western blot and indirect immunofluorescence one-plate detection device, and the technical scheme provided by the present invention is as follows:
the device comprises a base, a first detection device, a second detection device, an automatic sample feeding device, a sample adding treatment device and a reagent bottle area, wherein the first detection device is arranged on the base and used for detecting by adopting a western blotting method; the first detection device comprises a first incubation disc, a first waste discharge device arranged on the side edge of the first incubation disc, a first liquid injection device used for placing a sample to be processed, a first drying device used for drying the blotting membrane strip and a first scanning device used for collecting an image signal; the second detection device comprises a second incubation disc, a second waste discharge device arranged on the side edge of the second incubation disc, a second liquid injection device used for placing a sample to be processed, a second sealing device used for sealing the fluorescent sheet and a second scanning device used for collecting signals of the fluorescent sheet.
Preferably, the bottom of the first incubation disc and the bottom of the second incubation disc are respectively connected with a driving component for driving the first incubation disc and the second incubation disc to rotate, and the driving component is driven by a motor to rotate so as to drive the incubation discs to rotate.
preferably, the automatic sample feeding device comprises a sample cabin for placing samples and a sample cabin driving device for driving the sample cabin to run to the detection area, and the sample cabin driving device runs under the driving of a corresponding motor.
The invention has the advantages of realizing simultaneous western blot detection and fluorescence detection, automatically generating contrast data, greatly improving the working efficiency and saving manpower, material resources and financial resources.
Drawings
Fig. 1 is a top view of the overall structure of the present invention.
Detailed Description
The invention will be further explained and explained with reference to the drawings.
The device comprises a base 1, a first detection device 2 which is arranged on the base 1 and is used for detecting by a western blotting method, a second detection device 3 which is used for detecting by an immunofluorescence method, an automatic sample feeding device 4 which is used for providing a sample to be detected for the first detection device 2 and the second detection device 3, a sample adding processing device 5 which is matched with the first detection device 2 and the second detection device 3 and is used for adding the sample to be detected, and a reagent bottle area 6 which is used for loading a reagent bottle; the first detection device 2 comprises a first incubation disc 21, a first waste discharge device 22 arranged on the side edge of the first incubation disc 21, a first liquid injection device 23 used for placing a sample to be processed, a first drying device 24 used for drying the blotting membrane strip and a first scanning device 25 used for collecting an image signal; the second detection device 3 comprises a second incubation disc 31, a second waste discharge device 32 arranged on the side edge of the second incubation disc 31, a second liquid injection device 33 used for placing a sample to be processed, a second mounting device 34 used for mounting a fluorescent sheet and a second scanning device 35 used for collecting signals of the fluorescent sheet.
The bottom of the first incubation disc 21 and the bottom of the second incubation disc 31 are respectively connected with a driving component for driving the first incubation disc and the second incubation disc to rotate, and the driving component is driven by a motor to rotate so as to drive the incubation discs (21 and 31) to rotate.
The automatic sample introduction device comprises a sample bin for placing a sample and a sample bin driving device for driving the sample bin to run to the detection area, and the sample bin driving device runs under the driving of a corresponding motor.
The general procedure used is as follows:
1. A preparation stage: placing the reaction membrane strip or the fluorescent sheet on an incubation disc (21, 31) (the incubation disc can rotate), placing the sample in a sample bin on the automatic sample introduction device 4, and placing the reagent bottle in a reagent bottle area 6;
2. After the test is started, the automatic sample feeding device 4 conveys the sample tube to a sample feeding position, the sample feeding processing device 5 feeds a reagent and a sample to a blotting membrane strip or a fluorescent sheet on a reaction disc for a binding reaction, after the binding reaction is completed, the automatic sample feeding device 4 conveys the sample tube back to the original position, and the sample feeding processing device 5 cleans the needle;
3. After the reaction is finished, the waste discharge devices (32 and 22) suck away the reacted reagent, then the liquid injection devices (23 and 33) inject washing liquid to the blotting membrane strip or the fluorescent sheet, and then the waste discharge devices (32 and 22) suck away the liquid for waste discharge;
4. The sample adding processing device 5 sucks and adds the reagent from the reagent bottle area 6 to the blotting membrane strip or the fluorescent sheet of the reaction disk, and then the liquid is sucked away by the waste discharging devices (32, 22) after the reaction is finished;
5. repeating similar actions of adding washing liquid and adding reagent to complete sample treatment;
6. After all reactions are finished, if the blotting membrane strip method is adopted, the first drying device 24 can dry the blotting membrane strips on the incubation disc one by one (the incubation disc continuously rotates), then the first scanning device 25 collects image signals and sends the image signals to analysis software for analysis processing; if it is the fluorescence piece, second mounting device 34 can carry out the mounting to the fluorescence piece one by one, then gathers image signal by second scanning device 35, sends analysis software to carry out analysis processes, and so, advance the appearance application of sample and detect the analysis and can both accomplish automatically, and two dishes detect simultaneously, labour saving and time saving.
Claims (3)
1. The device for detecting the same tray by the western blotting method and the immunofluorescence method is characterized by comprising a base, a first detection device, a second detection device, an automatic sample feeding device, a sample adding treatment device and a reagent bottle area, wherein the first detection device is arranged on the base and used for detecting by the western blotting method, the second detection device is used for detecting by the immunofluorescence method, the automatic sample feeding device is used for providing a sample to be detected for the first detection device and the second detection device, the sample adding treatment device is matched with the first detection device and the second detection device for sample adding treatment of the sample to be detected, and the reagent bottle area is used for loading a reagent bottle; the first detection device comprises a first incubation disc, a first waste discharge device arranged on the side edge of the first incubation disc, a first liquid injection device used for placing a sample to be processed, a first drying device used for drying the blotting membrane strip and a first scanning device used for collecting an image signal; the second detection device comprises a second incubation disc, a second waste discharge device arranged on the side edge of the second incubation disc, a second liquid injection device used for placing a sample to be processed, a second sealing device used for sealing the fluorescent sheet and a second scanning device used for collecting signals of the fluorescent sheet.
2. the western blot and immunofluorescence same plate detection device according to claim 1, wherein the bottom of the first incubation plate and the bottom of the second incubation plate are respectively connected with a driving component for driving the first incubation plate and the second incubation plate to rotate.
3. The western blot and immunofluorescence same plate assay device according to claim 1, wherein the autosampler device comprises a sample compartment for holding a sample and a sample compartment driver for driving the sample compartment to travel to the detection zone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910957196.6A CN110554180A (en) | 2019-10-10 | 2019-10-10 | Same-tray detection device for protein blotting method and immunofluorescence method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910957196.6A CN110554180A (en) | 2019-10-10 | 2019-10-10 | Same-tray detection device for protein blotting method and immunofluorescence method |
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| Publication Number | Publication Date |
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| CN110554180A true CN110554180A (en) | 2019-12-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910957196.6A Pending CN110554180A (en) | 2019-10-10 | 2019-10-10 | Same-tray detection device for protein blotting method and immunofluorescence method |
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| Country | Link |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010090138A1 (en) * | 2009-02-03 | 2010-08-12 | ベックマン コールター インコーポレイテッド | Autoanalyzer and rack transfer method |
| CN103760375A (en) * | 2014-01-28 | 2014-04-30 | 桂林优利特医疗电子有限公司 | Improved automatic biochemical analyzer and usage method thereof |
| CN107966579A (en) * | 2017-12-26 | 2018-04-27 | 江苏柯伦迪医疗技术有限公司 | One kind has biochemistry, enzyme is exempted from and chemiluminescence rapid detection system and method |
| CN108120840A (en) * | 2018-01-19 | 2018-06-05 | 深圳市亚辉龙生物科技股份有限公司 | Full-automatic immunoblotting assay instrument |
| CN108459168A (en) * | 2018-03-27 | 2018-08-28 | 深圳市科创志达技术有限公司 | A kind of Full-automatic chemiluminescence immunoassay analysis meter |
| WO2018221220A1 (en) * | 2017-06-02 | 2018-12-06 | 株式会社日立ハイテクノロジーズ | Automatic analysis device |
| CN110221089A (en) * | 2019-07-08 | 2019-09-10 | 深圳市亚辉龙生物科技股份有限公司 | A kind of full-automatic Immunofluorescence test equipment |
| CN210775510U (en) * | 2019-10-10 | 2020-06-16 | 深圳市亚辉龙生物科技股份有限公司 | Same-tray detection device for protein blotting method and immunofluorescence method |
-
2019
- 2019-10-10 CN CN201910957196.6A patent/CN110554180A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010090138A1 (en) * | 2009-02-03 | 2010-08-12 | ベックマン コールター インコーポレイテッド | Autoanalyzer and rack transfer method |
| CN103760375A (en) * | 2014-01-28 | 2014-04-30 | 桂林优利特医疗电子有限公司 | Improved automatic biochemical analyzer and usage method thereof |
| WO2018221220A1 (en) * | 2017-06-02 | 2018-12-06 | 株式会社日立ハイテクノロジーズ | Automatic analysis device |
| CN107966579A (en) * | 2017-12-26 | 2018-04-27 | 江苏柯伦迪医疗技术有限公司 | One kind has biochemistry, enzyme is exempted from and chemiluminescence rapid detection system and method |
| CN108120840A (en) * | 2018-01-19 | 2018-06-05 | 深圳市亚辉龙生物科技股份有限公司 | Full-automatic immunoblotting assay instrument |
| CN108459168A (en) * | 2018-03-27 | 2018-08-28 | 深圳市科创志达技术有限公司 | A kind of Full-automatic chemiluminescence immunoassay analysis meter |
| CN110221089A (en) * | 2019-07-08 | 2019-09-10 | 深圳市亚辉龙生物科技股份有限公司 | A kind of full-automatic Immunofluorescence test equipment |
| CN210775510U (en) * | 2019-10-10 | 2020-06-16 | 深圳市亚辉龙生物科技股份有限公司 | Same-tray detection device for protein blotting method and immunofluorescence method |
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